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Conserved domains on  [gi|1063728862|ref|NP_198712|]
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RmlC-like cupins superfamily protein [Arabidopsis thaliana]

Protein Classification

germin family protein( domain architecture ID 14388826)

germin family protein is a cupin domain-containing protein similar to oxalate oxidase (also called germin) that catalyzes the manganese-dependent oxidative decarboxylation of oxalate to carbon dioxide and hydrogen peroxide

CATH:  2.60.120.10
Gene Ontology:  GO:0046872
PubMed:  14697267|19478949
SCOP:  3001825

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
cupin_OxOx cd02241
Oxalate oxidase (germin), cupin domain; Oxalate oxidase (OxOx, also known as germin; EC 1.2.3. ...
 26-222 2.86e-87

Oxalate oxidase (germin), cupin domain; Oxalate oxidase (OxOx, also known as germin; EC 1.2.3.4) catalyzes the manganese-dependent oxidative decarboxylation of oxalate to carbon dioxide and hydrogen peroxide (H2O2). It is widespread in fungi and various plant tissues and may play a role in plant signaling and defense. This enzyme has been employed in a widely used assay for detecting urinary oxalate levels. Also, the gene encoding OxOx from barley roots has been expressed in oilseed rape in order to provide a defense against externally supplied oxalic acid. In germin, the predominant protein produced during the early phase of wheat germination, it is believed that H2O2 production is employed as a defense mechanism in response to infection by pathogens. Germin is also a marker of growth onset in cell walls in germinating cereals. The H2O2 produced by OxOx, together with the Ca2+ released by degradation of calcium oxalate, are thought to mediate cell wall cross-linking at high concentrations. Proteins in this family belong to the cupin superfamily with a conserved "jelly roll-like" beta-barrel fold capable of homodimerization.


:

Pssm-ID: 380368  Cd Length: 191  Bit Score: 255.98  E-value: 2.86e-87
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063728862  26 DPSPLQDFCIGVNtpANALFVNGKFCKDPKLVTADDFYFSGLDKARTTeSSPVGSNVTTVNVNQIPGLNTLGISLVRIDY 105
Cdd:cd02241     1 DPDPLQDFCVADL--SSPRTPNGYPCKDPALVTADDFVFDFLNPPGNT-SNPLGGSVTLANVANFPALNGLGISMARGDL 77

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063728862 106 GINGQNPPHTHPRATEILLVQEGTLFVGFFSSfPENRLFNKTLNKGDVFVFPEGLIHFQVNIGKQPAVAFASLSSQNPGV 185
Cdd:cd02241    78 APCGVNPPHTHPRATELLYVVEGTLYVGFVDE-NGNRLFTKTLNPGDVFVFPQGLIHFQFNPGCEPAVFVAAFNSEDPGT 156

                         170       180       190
                  ....*....|....*....|....*....|....*..
gi 1063728862 186 IIIGNTLFGSKPPidPNVLAKAFQLDPKVIIQLQKKF 222
Cdd:cd02241   157 QQIAQALFGLPPP--DDVLAAAFGLDGAQVEKLKSKF 191
 
Name Accession Description Interval E-value
cupin_OxOx cd02241
Oxalate oxidase (germin), cupin domain; Oxalate oxidase (OxOx, also known as germin; EC 1.2.3. ...
 26-222 2.86e-87

Oxalate oxidase (germin), cupin domain; Oxalate oxidase (OxOx, also known as germin; EC 1.2.3.4) catalyzes the manganese-dependent oxidative decarboxylation of oxalate to carbon dioxide and hydrogen peroxide (H2O2). It is widespread in fungi and various plant tissues and may play a role in plant signaling and defense. This enzyme has been employed in a widely used assay for detecting urinary oxalate levels. Also, the gene encoding OxOx from barley roots has been expressed in oilseed rape in order to provide a defense against externally supplied oxalic acid. In germin, the predominant protein produced during the early phase of wheat germination, it is believed that H2O2 production is employed as a defense mechanism in response to infection by pathogens. Germin is also a marker of growth onset in cell walls in germinating cereals. The H2O2 produced by OxOx, together with the Ca2+ released by degradation of calcium oxalate, are thought to mediate cell wall cross-linking at high concentrations. Proteins in this family belong to the cupin superfamily with a conserved "jelly roll-like" beta-barrel fold capable of homodimerization.


Pssm-ID: 380368  Cd Length: 191  Bit Score: 255.98  E-value: 2.86e-87
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063728862  26 DPSPLQDFCIGVNtpANALFVNGKFCKDPKLVTADDFYFSGLDKARTTeSSPVGSNVTTVNVNQIPGLNTLGISLVRIDY 105
Cdd:cd02241     1 DPDPLQDFCVADL--SSPRTPNGYPCKDPALVTADDFVFDFLNPPGNT-SNPLGGSVTLANVANFPALNGLGISMARGDL 77

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063728862 106 GINGQNPPHTHPRATEILLVQEGTLFVGFFSSfPENRLFNKTLNKGDVFVFPEGLIHFQVNIGKQPAVAFASLSSQNPGV 185
Cdd:cd02241    78 APCGVNPPHTHPRATELLYVVEGTLYVGFVDE-NGNRLFTKTLNPGDVFVFPQGLIHFQFNPGCEPAVFVAAFNSEDPGT 156

                         170       180       190
                  ....*....|....*....|....*....|....*..
gi 1063728862 186 IIIGNTLFGSKPPidPNVLAKAFQLDPKVIIQLQKKF 222
Cdd:cd02241   157 QQIAQALFGLPPP--DDVLAAAFGLDGAQVEKLKSKF 191
Cupin_1 pfam00190
Cupin; This family represents the conserved barrel domain of the 'cupin' superfamily ('cupa' ...
 65-215 2.45e-59

Cupin; This family represents the conserved barrel domain of the 'cupin' superfamily ('cupa' is the Latin term for a small barrel). This family contains 11S and 7S plant seed storage proteins, and germins. Plant seed storage proteins provide the major nitrogen source for the developing plant.


Pssm-ID: 395138  Cd Length: 151  Bit Score: 183.69  E-value: 2.45e-59
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063728862  65 SGLDKARTTeSSPVGSNVTTVNVNQIPGLNTLGISLVRIDYGINGQNPPHTHPRATEILLVQEGTLFVGFFSSFPENRLF 144
Cdd:pfam00190   1 LNLLEPGPT-YNPEGGRVTTVNSKNLPGLNTLGISAARVDLAPGGMNPPHWHPNATEILYVLQGRGRVGFVVPGNGNRVF 79

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 1063728862 145 NKTLNKGDVFVFPEGLIHFQVNIGKQPAVAFASLSSQNPGVIIIGNTLFGSKPPIDPNVLAKAFQLDPKVI 215
Cdd:pfam00190  80 HKVLREGDVFVVPQGLPHFQYNIGDEPAVAFVAFDTNNPGNQSILAGGFSSLPALPPEVLAKAFQLAGEEV 150
Cupin_1 smart00835
Cupin; This family represents the conserved barrel domain of the 'cupin' superfamily ('cupa' ...
 82-215 4.16e-33

Cupin; This family represents the conserved barrel domain of the 'cupin' superfamily ('cupa' is the Latin term for a small barrel). This family contains 11S and 7S plant seed storage proteins, and germins. Plant seed storage proteins provide the major nitrogen source for the developing plant.


Pssm-ID: 214845 [Multi-domain]  Cd Length: 146  Bit Score: 116.61  E-value: 4.16e-33
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063728862   82 VTTVNVNQIPGLNTLGISLVRIDYGINGQNPPHTHPRATEILLVQEGTLFVGFFSSFPeNRLFNKTLNKGDVFVFPEGLI 161
Cdd:smart00835  14 LREADPTNFPALNGLGISAARVNLEPGGMLPPHYHPRATELLYVVRGEGRVGVVDPNG-NKVYDARLREGDVFVVPQGHP 92

                           90       100       110       120       130
                   ....*....|....*....|....*....|....*....|....*....|....
gi 1063728862  162 HFQVNIGKQPAVaFASLSSQNPGVIIIGNTLFGSKPPIDPNVLAKAFQLDPKVI 215
Cdd:smart00835  93 HFQVNSGDENLE-FVAFNTNDPNRRFFLAGRNSVLRGLPPEVLAAAFGVSAEEV 145
OxdD COG2140
Oxalate decarboxylase/archaeal phosphoglucose isomerase, cupin superfamily [Carbohydrate ...
 113-176 7.00e-09

Oxalate decarboxylase/archaeal phosphoglucose isomerase, cupin superfamily [Carbohydrate transport and metabolism]; Oxalate decarboxylase/archaeal phosphoglucose isomerase, cupin superfamily is part of the Pathway/BioSystem: Glycolysis


Pssm-ID: 441743 [Multi-domain]  Cd Length: 115  Bit Score: 51.89  E-value: 7.00e-09
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 1063728862 113 PHTHPRATEILLVQEGTLFVGFFSSFPENRLFNktLNKGDVFVFPEGLIHFQVNIGKQPAVAFA 176
Cdd:COG2140    18 EHWHPNAAEWYYVLSGEARMTVQDPPGRARTVD--VGPGDVVYVPPGYGHYIINTGDEPLVFLA 79
 
Name Accession Description Interval E-value
cupin_OxOx cd02241
Oxalate oxidase (germin), cupin domain; Oxalate oxidase (OxOx, also known as germin; EC 1.2.3. ...
 26-222 2.86e-87

Oxalate oxidase (germin), cupin domain; Oxalate oxidase (OxOx, also known as germin; EC 1.2.3.4) catalyzes the manganese-dependent oxidative decarboxylation of oxalate to carbon dioxide and hydrogen peroxide (H2O2). It is widespread in fungi and various plant tissues and may play a role in plant signaling and defense. This enzyme has been employed in a widely used assay for detecting urinary oxalate levels. Also, the gene encoding OxOx from barley roots has been expressed in oilseed rape in order to provide a defense against externally supplied oxalic acid. In germin, the predominant protein produced during the early phase of wheat germination, it is believed that H2O2 production is employed as a defense mechanism in response to infection by pathogens. Germin is also a marker of growth onset in cell walls in germinating cereals. The H2O2 produced by OxOx, together with the Ca2+ released by degradation of calcium oxalate, are thought to mediate cell wall cross-linking at high concentrations. Proteins in this family belong to the cupin superfamily with a conserved "jelly roll-like" beta-barrel fold capable of homodimerization.


Pssm-ID: 380368  Cd Length: 191  Bit Score: 255.98  E-value: 2.86e-87
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063728862  26 DPSPLQDFCIGVNtpANALFVNGKFCKDPKLVTADDFYFSGLDKARTTeSSPVGSNVTTVNVNQIPGLNTLGISLVRIDY 105
Cdd:cd02241     1 DPDPLQDFCVADL--SSPRTPNGYPCKDPALVTADDFVFDFLNPPGNT-SNPLGGSVTLANVANFPALNGLGISMARGDL 77

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063728862 106 GINGQNPPHTHPRATEILLVQEGTLFVGFFSSfPENRLFNKTLNKGDVFVFPEGLIHFQVNIGKQPAVAFASLSSQNPGV 185
Cdd:cd02241    78 APCGVNPPHTHPRATELLYVVEGTLYVGFVDE-NGNRLFTKTLNPGDVFVFPQGLIHFQFNPGCEPAVFVAAFNSEDPGT 156

                         170       180       190
                  ....*....|....*....|....*....|....*..
gi 1063728862 186 IIIGNTLFGSKPPidPNVLAKAFQLDPKVIIQLQKKF 222
Cdd:cd02241   157 QQIAQALFGLPPP--DDVLAAAFGLDGAQVEKLKSKF 191
Cupin_1 pfam00190
Cupin; This family represents the conserved barrel domain of the 'cupin' superfamily ('cupa' ...
 65-215 2.45e-59

Cupin; This family represents the conserved barrel domain of the 'cupin' superfamily ('cupa' is the Latin term for a small barrel). This family contains 11S and 7S plant seed storage proteins, and germins. Plant seed storage proteins provide the major nitrogen source for the developing plant.


Pssm-ID: 395138  Cd Length: 151  Bit Score: 183.69  E-value: 2.45e-59
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063728862  65 SGLDKARTTeSSPVGSNVTTVNVNQIPGLNTLGISLVRIDYGINGQNPPHTHPRATEILLVQEGTLFVGFFSSFPENRLF 144
Cdd:pfam00190   1 LNLLEPGPT-YNPEGGRVTTVNSKNLPGLNTLGISAARVDLAPGGMNPPHWHPNATEILYVLQGRGRVGFVVPGNGNRVF 79

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 1063728862 145 NKTLNKGDVFVFPEGLIHFQVNIGKQPAVAFASLSSQNPGVIIIGNTLFGSKPPIDPNVLAKAFQLDPKVI 215
Cdd:pfam00190  80 HKVLREGDVFVVPQGLPHFQYNIGDEPAVAFVAFDTNNPGNQSILAGGFSSLPALPPEVLAKAFQLAGEEV 150
Cupin_1 smart00835
Cupin; This family represents the conserved barrel domain of the 'cupin' superfamily ('cupa' ...
 82-215 4.16e-33

Cupin; This family represents the conserved barrel domain of the 'cupin' superfamily ('cupa' is the Latin term for a small barrel). This family contains 11S and 7S plant seed storage proteins, and germins. Plant seed storage proteins provide the major nitrogen source for the developing plant.


Pssm-ID: 214845 [Multi-domain]  Cd Length: 146  Bit Score: 116.61  E-value: 4.16e-33
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063728862   82 VTTVNVNQIPGLNTLGISLVRIDYGINGQNPPHTHPRATEILLVQEGTLFVGFFSSFPeNRLFNKTLNKGDVFVFPEGLI 161
Cdd:smart00835  14 LREADPTNFPALNGLGISAARVNLEPGGMLPPHYHPRATELLYVVRGEGRVGVVDPNG-NKVYDARLREGDVFVVPQGHP 92

                           90       100       110       120       130
                   ....*....|....*....|....*....|....*....|....*....|....
gi 1063728862  162 HFQVNIGKQPAVaFASLSSQNPGVIIIGNTLFGSKPPIDPNVLAKAFQLDPKVI 215
Cdd:smart00835  93 HFQVNSGDENLE-FVAFNTNDPNRRFFLAGRNSVLRGLPPEVLAAAFGVSAEEV 145
cupin_OxDC-like cd20306
Oxalate decarboxylase (OxDC)-like cupin domain; This subfamily contains bacterial and ...
 67-214 5.68e-13

Oxalate decarboxylase (OxDC)-like cupin domain; This subfamily contains bacterial and eukaryotic cupin domains of proteins homologous to oxalate decarboxylase (OxDC; EC 4.1.1.2) such as MSMEG_2254, a putative OxDC from Mycobacterium smegmatis. OxDC is a manganese-dependent bicupin that catalyzes the conversion of oxalate to formate and carbon dioxide, utilizing dioxygen as a cofactor. It is evolutionarily related to oxalate oxidase (OxOx or germin; EC 1.2.3.4) which, in contrast, converts oxalate and dioxygen to carbon dioxide and hydrogen peroxide. OxDC is classified as a bicupin because it contains two cupin folds with each domain containing one manganese binding site, with four manganese binding residues (three histidines and one glutamate) conserved as well as a number of hydrophobic residues.


Pssm-ID: 380440 [Multi-domain]  Cd Length: 151  Bit Score: 64.15  E-value: 5.68e-13
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063728862  67 LDKARTTESSPVGSnVTTVNVNQIPGLNtlGISLVRIDYGINGQNPPHTHPRATEILLVQEGTLFVGFFSsfPENRLFNK 146
Cdd:cd20306     6 LEDSNPFFESEGGS-IRQATADQLPVLK--GLSIYRLRLSPGGIREPHWHPNANELGYVISGEARVSILD--PTGSLDTF 80

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 1063728862 147 TLNKGDVFVFPEGLIHFQVNIGKQPAVAFASLSSQNPGVIIIGNTLfgSKPPidPNVLAKAFQLDPKV 214
Cdd:cd20306    81 TVKPGQVVFIPQGWLHWIENVGDEEAHLLIFFNHETPEDIGLSDSL--RATP--PEVLGNTYGVDAFF 144
cupin_OxDC cd02240
Oxalate decarboxylase (OxDC), cupin domain; Oxalate decarboxylase (OxDC; EC 4.1.1.2) is a ...
 74-173 2.51e-10

Oxalate decarboxylase (OxDC), cupin domain; Oxalate decarboxylase (OxDC; EC 4.1.1.2) is a manganese-dependent bicupin that catalyzes the conversion of oxalate to formate and carbon dioxide, utilizing dioxygen as a cofactor. It is evolutionarily related to oxalate oxidase (OxOx or germin; EC 1.2.3.4) which, in contrast, converts oxalate and dioxygen to carbon dioxide and hydrogen peroxide. OxDC is classified as a bicupin because it contains two cupin folds and both domains are included in this alignment. Each OxDC cupin domain contains one manganese binding site, with four manganese binding residues (three histidines and one glutamate) conserved as well as a number of hydrophobic residues. Members of this family belong to the cupin superfamily with a conserved "jelly roll-like" beta-barrel fold.


Pssm-ID: 380367 [Multi-domain]  Cd Length: 145  Bit Score: 56.72  E-value: 2.51e-10
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063728862  74 ESSPVGSnVTTVNVNQIPGLNTLGISLVRIDYGinGQNPPHTHPRATEILLVQEGTLFVGFFSsfPENRLFNKTLNKGDV 153
Cdd:cd02240     6 EENAGGS-VRIATVTNFPISKDLSSALVRVAPG--AMRELHWHPNTAEWQYVISGSARVTVFD--EDGRFETFNLGAGDV 80

                          90       100
                  ....*....|....*....|
gi 1063728862 154 FVFPEGLIHFQVNIGKQPAV 173
Cdd:cd02240    81 GYVPSGSGHHIENIGDEDAE 100
OxdD COG2140
Oxalate decarboxylase/archaeal phosphoglucose isomerase, cupin superfamily [Carbohydrate ...
 113-176 7.00e-09

Oxalate decarboxylase/archaeal phosphoglucose isomerase, cupin superfamily [Carbohydrate transport and metabolism]; Oxalate decarboxylase/archaeal phosphoglucose isomerase, cupin superfamily is part of the Pathway/BioSystem: Glycolysis


Pssm-ID: 441743 [Multi-domain]  Cd Length: 115  Bit Score: 51.89  E-value: 7.00e-09
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 1063728862 113 PHTHPRATEILLVQEGTLFVGFFSSFPENRLFNktLNKGDVFVFPEGLIHFQVNIGKQPAVAFA 176
Cdd:COG2140    18 EHWHPNAAEWYYVLSGEARMTVQDPPGRARTVD--VGPGDVVYVPPGYGHYIINTGDEPLVFLA 79
cupin_RmlC-like cd02208
RmlC-like cupin superfamily; This superfamily contains proteins similar to the RmlC (dTDP ...
 98-175 1.89e-08

RmlC-like cupin superfamily; This superfamily contains proteins similar to the RmlC (dTDP (deoxythymidine diphosphates)-4-dehydrorhamnose 3,5-epimerase)-like cupins. RmlC is a dTDP-sugar isomerase involved in the synthesis of L-rhamnose, a saccharide required for the virulence of some pathogenic bacteria. Cupins are a functionally diverse superfamily originally discovered based on the highly conserved motif found in germin and germin-like proteins. This conserved motif forms a beta-barrel fold found in all of the cupins, giving rise to the name cupin ('cupa' is the Latin term for small barrel). The active site of members of this superfamily is generally located at the center of a conserved barrel and usually includes a metal ion. The different functional classes in this superfamily include single domain bacterial isomerases and epimerases involved in the modification of cell wall carbohydrates, two domain bicupins such as the desiccation-tolerant seed storage globulins, and multidomain nuclear transcription factors involved in legume root nodulation.


Pssm-ID: 380338 [Multi-domain]  Cd Length: 73  Bit Score: 49.79  E-value: 1.89e-08
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 1063728862  98 ISLVRIDYGinGQNPPHTHPRATEILLVQEGTLFVGFFSSFPENrlfnktLNKGDVFVFPEGLIHFQVNIGKQPAVAF 175
Cdd:cd02208     1 ISVVTLPPG--TSSPPHWHPEQDEIFYVLSGEGELTLDDGETVE------LKAGDIVLIPPGVPHSFVNTSDEPAVFL 70
ManC COG0662
Mannose-6-phosphate isomerase, cupin superfamily [Carbohydrate transport and metabolism];
97-173 6.36e-07

Mannose-6-phosphate isomerase, cupin superfamily [Carbohydrate transport and metabolism];


Pssm-ID: 440426 [Multi-domain]  Cd Length: 114  Bit Score: 46.67  E-value: 6.36e-07
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 1063728862  97 GISLVRIDYGINGQNPPHTHPRATEILLVQEGTLFVGFfssfpENRLFnkTLNKGDVFVFPEGLIHFQVNIGKQPAV 173
Cdd:COG0662    26 RLSVKRITVPPGAELSLHVHPHRDEFFYVLEGTGEVTI-----GDEEV--ELKAGDSVYIPAGVPHRLRNPGDEPLE 95
QdoI COG1917
Cupin domain protein related to quercetin dioxygenase [General function prediction only];
98-176 1.40e-06

Cupin domain protein related to quercetin dioxygenase [General function prediction only];


Pssm-ID: 441521 [Multi-domain]  Cd Length: 99  Bit Score: 45.22  E-value: 1.40e-06
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 1063728862  98 ISLVRIDYGINGQNPPHTHPrATEILLVQEGTLFVGFfssfpENRLFnkTLNKGDVFVFPEGLIHFQVNIGKQPAVAFA 176
Cdd:COG1917    23 LEVVRVTFEPGARTPWHSHP-GEELIYVLEGEGEVEV-----GGEEY--ELKPGDVVFIPPGVPHAFRNLGDEPAVLLV 93
Cupin_2 pfam07883
Cupin domain; This family represents the conserved barrel domain of the 'cupin' superfamily ( ...
 100-176 2.42e-06

Cupin domain; This family represents the conserved barrel domain of the 'cupin' superfamily ('cupa' is the Latin term for a small barrel).


Pssm-ID: 462300 [Multi-domain]  Cd Length: 71  Bit Score: 43.79  E-value: 2.42e-06
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 1063728862 100 LVRIDYGinGQNPPHTHPRATEILLVQEGTLFVgffsSFPENRLfnkTLNKGDVFVFPEGLIHFQVNIGKQPAVAFA 176
Cdd:pfam07883   2 LVTLPPG--ESSPPHRHPGEDEFFYVLEGEGEL----TVDGEEV---VLKAGDSVYFPAGVPHRFRNTGDEPARLLD 69
COG3837 COG3837
Uncharacterized conserved protein, cupin superfamily [Function unknown];
92-176 9.02e-06

Uncharacterized conserved protein, cupin superfamily [Function unknown];


Pssm-ID: 443048 [Multi-domain]  Cd Length: 115  Bit Score: 43.47  E-value: 9.02e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063728862  92 GLNTLGISLVRIDYGiNGQNPPHTHPRATEILLVQEGTLFVgffsSFPENRLfnkTLNKGDVFVFPEGLIHFQVNIGKQP 171
Cdd:COG3837    24 GLTRLGVNLITLPPG-ASSSPYHAHSAEEEFVYVLEGELTL----RIGGEEY---VLEPGDSVGFPAGVPHRLRNRGDEP 95


                  ....*
gi 1063728862 172 AVAFA 176
Cdd:COG3837    96 ARYLV 100
cupin_OxDC_C cd20305
Oxalate decarboxylase (OxDC), C-terminal cupin domain; This model represents the C-terminal ...
 67-221 2.80e-05

Oxalate decarboxylase (OxDC), C-terminal cupin domain; This model represents the C-terminal cupin domain of oxalate decarboxylase (OxDC; EC 4.1.1.2), a manganese-dependent bicupin that catalyzes the conversion of oxalate to formate and carbon dioxide, utilizing dioxygen as a cofactor. It is evolutionarily related to oxalate oxidase (OxOx or germin; EC 1.2.3.4) which, in contrast, converts oxalate and dioxygen to carbon dioxide and hydrogen peroxide. OxDC is classified as a bicupin because it contains two cupin folds with each domain containing one manganese binding site, with four manganese binding residues (three histidines and one glutamate) conserved as well as a number of hydrophobic residues. Members of this family belong to the cupin superfamily with a conserved "jelly roll-like" beta-barrel fold.


Pssm-ID: 380439 [Multi-domain]  Cd Length: 153  Bit Score: 42.96  E-value: 2.80e-05
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063728862  67 LDKARTTESSPVGSnVTTVNVNQIPGLNTLGISLVRIDYGinGQNPPHTHPRATEILLVQEGTLFVGFFSSFPENRLFNk 146
Cdd:cd20305     6 LLAQPPIKVPAGGS-VRIVDSKNFPISTTIAAALVTLEPG--ALRELHWHPNADEWQYYISGKARMTVFASGGRARTFD- 81

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063728862 147 tLNKGDVFVFPEGLIHFQVNIGKQPAV--------AFASLS-SQ----NPgviiigntlfgskppidPNVLAKAFQLDPK 213
Cdd:cd20305    82 -FQAGDVGYVPRGYGHYIENTGDEPLEflevfnsgRYQDISlSQwlalTP-----------------PDLVAAHLGLPDD 143


                  ....*...
gi 1063728862 214 VIIQLQKK 221
Cdd:cd20305   144 TIAKLPKK 151
cupin_yp_001338853-like cd07008
Klebsiella pneumoniae yp_001338853.1 and related proteins, cupin domain; This family includes ...
 109-178 3.71e-05

Klebsiella pneumoniae yp_001338853.1 and related proteins, cupin domain; This family includes bacterial proteins homologous to Klebsiella pneumoniae yp_001338853.1, an uncharacterized conserved protein with double-stranded beta-helix domain. Proteins in this family belong to the cupin superfamily with a conserved "jelly roll-like" beta-barrel fold capable of homodimerization.


Pssm-ID: 380411 [Multi-domain]  Cd Length: 101  Bit Score: 41.47  E-value: 3.71e-05
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 1063728862 109 GQN-PPHTHPRATEILLVQEGTlfvGFFSSFPENRlfnKTLNKGDVFVFPEGLIHFQVNIGKQPAVaFASL 178
Cdd:cd07008    37 GQEiAAHIHPHGQDTWIVLSGE---GEYLLGDGQT---VPIKAGDIVIAPAGQVHGARNTGDEPLV-FVSV 100
cupin_DRT102 cd06989
Arabidopsis thaliana DRT102 and related proteins, cupin domain; This family includes bacterial ...
 97-163 4.88e-05

Arabidopsis thaliana DRT102 and related proteins, cupin domain; This family includes bacterial and eukaryotic proteins homologous to DNA-damage-repair/toleration protein DRT102 found in Arabidopsis thaliana. DRT102 may be involved in DNA repair from UV damage. Proteins in this family belong to the cupin superfamily with a conserved "jelly roll-like" beta-barrel fold capable of homodimerization.


Pssm-ID: 380394  Cd Length: 97  Bit Score: 40.98  E-value: 4.88e-05
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 1063728862  97 GISLVRIDYGINGQNPPHTHPrATEILLVQEGTLFVGFFSSFPENRLfnKTLNKGDVFVFPEGLIHF 163
Cdd:cd06989    17 GPFVVRLKFPAGYKIPPHTHP-DDERVTVISGTFYLGMGDKFDEAKA--KALPAGSFFTLPAGTPHF 80
cupin_YdbB-like cd02226
Bacillus subtilis YdbB and related proteins, cupin domain; This family includes bacterial ...
 114-162 6.84e-05

Bacillus subtilis YdbB and related proteins, cupin domain; This family includes bacterial proteins homologous to YdbB, a Bacillus subtilis protein of unknown function. It also includes protein Nmb1881 From Neisseria meningitidis, also of unknown function. Proteins in this family belong to the cupin superfamily with a conserved "jelly roll-like" beta-barrel fold capable of homodimerization.


Pssm-ID: 380355 [Multi-domain]  Cd Length: 94  Bit Score: 40.50  E-value: 6.84e-05
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|....*....
gi 1063728862 114 HTHPRATEILLVQEGTLFVGFfssfpENRlfNKTLNKGDVFVFPEGLIH 162
Cdd:cd02226    38 HKHDDEDELFLVLEGELTIDF-----RDR--DVTLGPGEFFVVPKGVEH 79
cupin_MAE_RS03005 cd06987
Microcystis aeruginosa MAE_RS03005 and related proteins, cupin domain; This family includes ...
 109-170 7.72e-05

Microcystis aeruginosa MAE_RS03005 and related proteins, cupin domain; This family includes bacterial and some eukaryotic proteins homologous to MAE_RS03005, a Microcystis aeruginosa protein of unknown function. Proteins in this family belong to the cupin superfamily with a conserved "jelly roll-like" beta-barrel fold capable of homodimerization.


Pssm-ID: 380392 [Multi-domain]  Cd Length: 122  Bit Score: 41.10  E-value: 7.72e-05
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 1063728862 109 GQNPPHTHPRATEILLV--QEGTLFVGffssfpENRLfnkTLNKGDVFVFPEGLIHFQVNIGKQ 170
Cdd:cd06987    39 GRTPPNTHPAAHEMFFVlaGEGRAYCD------GQRV---PLRPGDALVVPPGSEHVIENTGSG 93
cupin_BLL4011-like cd02235
Bradyrhizobium diazoefficiens BLL4011 and related proteins, cupin domain; This family includes ...
 100-176 9.08e-05

Bradyrhizobium diazoefficiens BLL4011 and related proteins, cupin domain; This family includes bacterial and fungal proteins homologous to BLL4011, a Bradyrhizobium diazoefficiens protein of unknown function. Proteins in this family belong to the cupin superfamily with a conserved "jelly roll-like" beta-barrel fold capable of homodimerization.


Pssm-ID: 380363 [Multi-domain]  Cd Length: 100  Bit Score: 40.25  E-value: 9.08e-05
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 1063728862 100 LVRIDYGINGQNPPHTHPrATEILLVQEGTLfVGFFSSFPEnrlfnKTLNKGDVFVFPEGLIHFQVNIGKQPAVAFA 176
Cdd:cd02235    21 QVRVEIPPGAVAGRHTHP-GEESGYVLEGSL-ELEVDGQPP-----VTLKAGDSFFIPAGTVHNAKNVGSGPAKLLA 90
cupin_PA3510-like cd02225
Pseudomonas aeruginosa PA3510 and related proteins, cupin domain; This family includes ...
 111-187 1.95e-04

Pseudomonas aeruginosa PA3510 and related proteins, cupin domain; This family includes bacterial proteins homologous to PA3510, a Pseudomonas aeruginosa protein of unknown function with a beta-barrel fold that belongs to the cupin superfamily.


Pssm-ID: 380354  Cd Length: 150  Bit Score: 40.34  E-value: 1.95e-04
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 1063728862 111 NPPHTHPrATEILLVQEG--TLFVGffssfPENRLFNKTLNKGDVFVFPEGLIHFQVNIGKQPAVAFASLSSQNPGVII 187
Cdd:cd02225    66 GALHTHE-VEEVFFVLQGrlTVFWE-----DEGEEHERELGPRDMISVPAGVYRGFKNIGEEDALMQVMLGTGKPGRPT 138
cupin_QDO_N_C cd02215
quercetinase, N- and C-terminal cupin domains; This family contains quercetinase (also known ...
 78-165 1.50e-03

quercetinase, N- and C-terminal cupin domains; This family contains quercetinase (also known as quercetin 2,3-dioxygenase, 2,3QD, QDO and YxaG; EC 1.13.11.24), a mononuclear copper-dependent dioxygenase that catalyzes the cleavage of the flavonol quercetin (5,7,3',4'-tetrahydroxyflavonol) heterocyclic ring to produce 2-protocatechuoyl-phloroglucinol carboxylic acid and carbon monoxide. Bacillus subtilis quercetin 2,3-dioxygenase (QDO) is a homodimer that shows oxygenase activity with several divalent metals such as Mn2+, Co2+, Fe2+, and Cu2+, although the preferred one appears to be Mn2+. The dioxygen binds to the metal ion of the Cu-QDO-quercetin complex, yielding a Cu2+-superoxo quercetin radical intermediate, which then forms a Cu2+-alkylperoxo complex which then evolves into endoperoxide intermediate that decomposes to the product. Quercetinase is a bicupin with two tandem cupin beta-barrel domains, both of which are included in this alignment model. The pirins, which also belong to the cupin domain family, have been shown to catalyze a reaction involving quercetin and may have a function similar to that of quercetinase.


Pssm-ID: 380345 [Multi-domain]  Cd Length: 122  Bit Score: 37.13  E-value: 1.50e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063728862  78 VGSNVTTVNVNqipGLNTLG-ISLVRIDYGINGQNPPHTHPRATEILLVQEG--TLFVGffssfPENRLfnktLNKGDvF 154
Cdd:cd02215    14 VGGQLFTVLAT---GESTGGaFTLVTTEGPKGDAIPPHYHKRHHETFYVLEGrlQLWLD-----GESRL----LTPGD-F 80

                          90
                  ....*....|...
gi 1063728862 155 VF-PEGLIH-FQV 165
Cdd:cd02215    81 ASvPPGTIHaYRM 93
cupin_7S_11S_C cd20285
7S and 11S seed storage globulin, C-terminal cupin domain; This family contains the C-terminal ...
 81-157 3.65e-03

7S and 11S seed storage globulin, C-terminal cupin domain; This family contains the C-terminal cupin domains of 7S and 11S seed storage proteins. The 7S globulins include soybean allergen beta-conglycinin, peanut allergen conarachin (Ara h 1), walnut allergen Jug r 2, and lentil allergen Len c 1. Proteins in this family perform various functions, including a role in sucrose binding, desiccation, defense against microbes and oxidative stress. The 11S globulins include many common food allergens such as the peanut major allergen Ara h 3, almond allergen Pru du 6, pecan allergen Car i 4, hazelnut nut allergen Cor a 9, Brazil nut allergen Ber e 2, cashew allergen Ana o 2, pistachio allergen Pis v 2/5, and walnut allergen Jug n/r 4. These plant seed storage globulins have tandem cupin-like beta-barrel folds (referred to as a bicupin). Storage proteins are the cause of well-known allergic reactions to peanuts and cereals. Proteins in this family belong to the cupin superfamily with a conserved "jelly roll-like" beta-barrel fold.


Pssm-ID: 380420  Cd Length: 109  Bit Score: 36.05  E-value: 3.65e-03
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 1063728862  81 NVTTVNVNQIPGLNTLGISLVRIDYGINGQNPPHTHPRATEILLVQEGTLFVGFFSSfPENRLFNKTLNKGDVFVFP 157
Cdd:cd20285     2 NVTERTSNDFPILKSLNLLASSISLEEGAMFVPHYYSKAIVILVVNEGRAHIQVVGP-KGYESYDAELSKGDVFVVP 77
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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