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Conserved domains on  [gi|15236280|ref|NP_195241|]
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O-methyltransferase family protein [Arabidopsis thaliana]

Protein Classification

class I SAM-dependent methyltransferase( domain architecture ID 10547661)

class I SAM-dependent methyltransferase catalyzes the methylation of one or more specific substrates using S-adenosyl-L-methionine (SAM or AdoMet) as the methyl donor; similar to Humulus lupulus xanthohumol 4-O-methyltransferase and O-methyltransferase 3

CATH:  2.20.25.110
EC:  2.1.1.-
Gene Ontology:  GO:1904047|GO:0008168|GO:0032259|GO:0008757
PubMed:  12504684|12826405
SCOP:  3000118

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
AdoMet_MTases super family cl17173
S-adenosylmethionine-dependent methyltransferases (SAM or AdoMet-MTase), class I; ...
 108-306 1.27e-47

S-adenosylmethionine-dependent methyltransferases (SAM or AdoMet-MTase), class I; AdoMet-MTases are enzymes that use S-adenosyl-L-methionine (SAM or AdoMet) as a substrate for methyltransfer, creating the product S-adenosyl-L-homocysteine (AdoHcy). There are at least five structurally distinct families of AdoMet-MTases, class I being the largest and most diverse. Within this class enzymes can be classified by different substrate specificities (small molecules, lipids, nucleic acids, etc.) and different target atoms for methylation (nitrogen, oxygen, carbon, sulfur, etc.).


The actual alignment was detected with superfamily member pfam00891:

Pssm-ID: 473071 [Multi-domain]  Cd Length: 208  Bit Score: 159.11  E-value: 1.27e-47
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15236280   108 KLHGKDLWAFAQDNLCHSQLINEAMACDARRVVPRVAGACQglFDGVATVVDVGGGTGETMGILVKEFPWIKGFNFDLPH 187
Cdd:pfam00891  17 KAFGISLFEAIYRDEEERLLFNRGLQEHWSLIGKDVLTAFD--LSGFRSLVDVGGGTGALAQAIVSLYPGCKGIVFDLPH 94

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15236280   188 VIEVA------QVLDGVENVEGDMF-DSIPASDAVIIKWVLHDWGDKDCIKILKNCKEAVLPNiGKVLIVECVIGEKKNT 260
Cdd:pfam00891  95 VVEAApthfsaGEEPRVTFHGGDFFkDSLPEADAYILKRVLHDWSDEKCVKLLKRCYKACPAG-GKVILVESLLGADPSG 173

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|....*.
gi 15236280   261 MIAEErddklehvrlQLDMVMMVHTsTGKERTLKEWDFVLTEAGFA 306
Cdd:pfam00891 174 PLHTQ----------LYSLNMLAQT-EGRERTEAEYSELLTGAGFS 208
dimerization pfam08100
dimerization domain; This domain is found at the N-terminus of a variety of plant ...
 33-77 4.42e-08

dimerization domain; This domain is found at the N-terminus of a variety of plant O-methyltransferases. It has been shown to mediate dimerization of these proteins.


:

Pssm-ID: 400439  Cd Length: 50  Bit Score: 48.73  E-value: 4.42e-08
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|....*....
gi 15236280    33 KCAIDLKIPEAIENHpsSQPVTLSELSSAVSASPSH----LRRIMRFLV 77
Cdd:pfam08100   4 KCAIELGIPDIIAKH--GKPLSPSELASKLPTKNPEapvmLDRLLRLLA 50
 
Name Accession Description Interval E-value
Methyltransf_2 pfam00891
O-methyltransferase domain; This family includes a range of O-methyltransferases. These ...
 108-306 1.27e-47

O-methyltransferase domain; This family includes a range of O-methyltransferases. These enzymes utilize S-adenosyl methionine.


Pssm-ID: 395719 [Multi-domain]  Cd Length: 208  Bit Score: 159.11  E-value: 1.27e-47
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15236280   108 KLHGKDLWAFAQDNLCHSQLINEAMACDARRVVPRVAGACQglFDGVATVVDVGGGTGETMGILVKEFPWIKGFNFDLPH 187
Cdd:pfam00891  17 KAFGISLFEAIYRDEEERLLFNRGLQEHWSLIGKDVLTAFD--LSGFRSLVDVGGGTGALAQAIVSLYPGCKGIVFDLPH 94

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15236280   188 VIEVA------QVLDGVENVEGDMF-DSIPASDAVIIKWVLHDWGDKDCIKILKNCKEAVLPNiGKVLIVECVIGEKKNT 260
Cdd:pfam00891  95 VVEAApthfsaGEEPRVTFHGGDFFkDSLPEADAYILKRVLHDWSDEKCVKLLKRCYKACPAG-GKVILVESLLGADPSG 173

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|....*.
gi 15236280   261 MIAEErddklehvrlQLDMVMMVHTsTGKERTLKEWDFVLTEAGFA 306
Cdd:pfam00891 174 PLHTQ----------LYSLNMLAQT-EGRERTEAEYSELLTGAGFS 208
dimerization pfam08100
dimerization domain; This domain is found at the N-terminus of a variety of plant ...
 33-77 4.42e-08

dimerization domain; This domain is found at the N-terminus of a variety of plant O-methyltransferases. It has been shown to mediate dimerization of these proteins.


Pssm-ID: 400439  Cd Length: 50  Bit Score: 48.73  E-value: 4.42e-08
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|....*....
gi 15236280    33 KCAIDLKIPEAIENHpsSQPVTLSELSSAVSASPSH----LRRIMRFLV 77
Cdd:pfam08100   4 KCAIELGIPDIIAKH--GKPLSPSELASKLPTKNPEapvmLDRLLRLLA 50
IscR COG1959
DNA-binding transcriptional regulator, IscR family [Transcription];
47-81 4.16e-05

DNA-binding transcriptional regulator, IscR family [Transcription];


Pssm-ID: 441562  Cd Length: 141  Bit Score: 42.90  E-value: 4.16e-05
                        10        20        30
                ....*....|....*....|....*....|....*
gi 15236280  47 HPSSQPVTLSELSSAVSASPSHLRRIMRFLVHQGL 81
Cdd:COG1959  19 HPGGEPVTSKEIAERQGISPSYLEKILQKLRKAGL 53
methyltran_NanM TIGR04371
putative sugar O-methyltransferase; Members of this family appear to be SAM-dependent ...
 147-193 9.62e-04

putative sugar O-methyltransferase; Members of this family appear to be SAM-dependent O-methyltransferases acting on sugars, based on iterated sequence searches and gene context. Members occur in Leptospira O-antigen regions, as well NanM from the biosynthesis cluster for nanchangmycin, which produces 4-O-methyl-L-rhodinose as an intermediate. [Cell envelope, Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides]


Pssm-ID: 275164  Cd Length: 273  Bit Score: 40.09  E-value: 9.62e-04
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|....*....
gi 15236280   147 CQGLFDG--VATVVDVGGGTGETMGILVKEFPWIKGFNFDLPHVIEVAQ 193
Cdd:TIGR04371 141 LKKFFGDlsVFRVLEIGGGYGRLGEILLKLFPNAIYYIVDLPPQLALSE 189
SmtA COG0500
SAM-dependent methyltransferase [Secondary metabolites biosynthesis, transport and catabolism, ...
 136-324 1.05e-03

SAM-dependent methyltransferase [Secondary metabolites biosynthesis, transport and catabolism, General function prediction only];


Pssm-ID: 440266 [Multi-domain]  Cd Length: 199  Bit Score: 39.51  E-value: 1.05e-03
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15236280 136 ARRVVPRVAGACQGL--FDGVATVVDVGGGTGETMGILVKEFPWiKGFNFDL-PHVIEVAQ------VLDGVENVEGDMF 206
Cdd:COG0500   7 SDELLPGLAALLALLerLPKGGRVLDLGCGTGRNLLALAARFGG-RVIGIDLsPEAIALARaraakaGLGNVEFLVADLA 85

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15236280 207 DSIPAS----DAVIIKWVLHDWGDKDCIKILKNCKEAVLPNiGKVLIVecvigekkNTMIAEERDDKlEHVRLQLDMVMM 282
Cdd:COG0500  86 ELDPLPaesfDLVVAFGVLHHLPPEEREALLRELARALKPG-GVLLLS--------ASDAAAALSLA-RLLLLATASLLE 155

                       170       180       190       200
                ....*....|....*....|....*....|....*....|..
gi 15236280 283 VHTSTGKERTLKEwDFVLTEAGFARYEVRDFDDVQSLIIAYR 324
Cdd:COG0500 156 LLLLLRLLALELY-LRALLAAAATEDLRSDALLESANALEYL 196
 
Name Accession Description Interval E-value
Methyltransf_2 pfam00891
O-methyltransferase domain; This family includes a range of O-methyltransferases. These ...
 108-306 1.27e-47

O-methyltransferase domain; This family includes a range of O-methyltransferases. These enzymes utilize S-adenosyl methionine.


Pssm-ID: 395719 [Multi-domain]  Cd Length: 208  Bit Score: 159.11  E-value: 1.27e-47
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15236280   108 KLHGKDLWAFAQDNLCHSQLINEAMACDARRVVPRVAGACQglFDGVATVVDVGGGTGETMGILVKEFPWIKGFNFDLPH 187
Cdd:pfam00891  17 KAFGISLFEAIYRDEEERLLFNRGLQEHWSLIGKDVLTAFD--LSGFRSLVDVGGGTGALAQAIVSLYPGCKGIVFDLPH 94

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15236280   188 VIEVA------QVLDGVENVEGDMF-DSIPASDAVIIKWVLHDWGDKDCIKILKNCKEAVLPNiGKVLIVECVIGEKKNT 260
Cdd:pfam00891  95 VVEAApthfsaGEEPRVTFHGGDFFkDSLPEADAYILKRVLHDWSDEKCVKLLKRCYKACPAG-GKVILVESLLGADPSG 173

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|....*.
gi 15236280   261 MIAEErddklehvrlQLDMVMMVHTsTGKERTLKEWDFVLTEAGFA 306
Cdd:pfam00891 174 PLHTQ----------LYSLNMLAQT-EGRERTEAEYSELLTGAGFS 208
dimerization pfam08100
dimerization domain; This domain is found at the N-terminus of a variety of plant ...
 33-77 4.42e-08

dimerization domain; This domain is found at the N-terminus of a variety of plant O-methyltransferases. It has been shown to mediate dimerization of these proteins.


Pssm-ID: 400439  Cd Length: 50  Bit Score: 48.73  E-value: 4.42e-08
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|....*....
gi 15236280    33 KCAIDLKIPEAIENHpsSQPVTLSELSSAVSASPSH----LRRIMRFLV 77
Cdd:pfam08100   4 KCAIELGIPDIIAKH--GKPLSPSELASKLPTKNPEapvmLDRLLRLLA 50
Rrf2 pfam02082
Iron-dependent Transcriptional regulator; Several proteins in this family form iron-sulfur ...
 44-96 1.22e-05

Iron-dependent Transcriptional regulator; Several proteins in this family form iron-sulfur clusters enabling iron dependent DNA transcription regulation. The iron binding is mediated by three conserved cysteine residues. Members of this family can also bind O-acetyl-L-serine, [Fe-S] and nitric oxide (Matilla et. al., FEMS Microbiology Reviews, fuab043, 45, 2021, 1. https://doi.org/10.1093/femsre/fuab043).


Pssm-ID: 396591 [Multi-domain]  Cd Length: 131  Bit Score: 44.08  E-value: 1.22e-05
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|...
gi 15236280    44 IENHPSSQPVTLSELSSAVSASPSHLRRIMRFLVHQGLFKEVPTKDGlatGYT 96
Cdd:pfam02082  15 LALHPGGEPVTSEEIAERQNISPVYLEKILAKLRKAGLVESVRGAGG---GYR 64
IscR COG1959
DNA-binding transcriptional regulator, IscR family [Transcription];
47-81 4.16e-05

DNA-binding transcriptional regulator, IscR family [Transcription];


Pssm-ID: 441562  Cd Length: 141  Bit Score: 42.90  E-value: 4.16e-05
                        10        20        30
                ....*....|....*....|....*....|....*
gi 15236280  47 HPSSQPVTLSELSSAVSASPSHLRRIMRFLVHQGL 81
Cdd:COG1959  19 HPGGEPVTSKEIAERQGISPSYLEKILQKLRKAGL 53
methyltran_NanM TIGR04371
putative sugar O-methyltransferase; Members of this family appear to be SAM-dependent ...
 147-193 9.62e-04

putative sugar O-methyltransferase; Members of this family appear to be SAM-dependent O-methyltransferases acting on sugars, based on iterated sequence searches and gene context. Members occur in Leptospira O-antigen regions, as well NanM from the biosynthesis cluster for nanchangmycin, which produces 4-O-methyl-L-rhodinose as an intermediate. [Cell envelope, Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides]


Pssm-ID: 275164  Cd Length: 273  Bit Score: 40.09  E-value: 9.62e-04
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|....*....
gi 15236280   147 CQGLFDG--VATVVDVGGGTGETMGILVKEFPWIKGFNFDLPHVIEVAQ 193
Cdd:TIGR04371 141 LKKFFGDlsVFRVLEIGGGYGRLGEILLKLFPNAIYYIVDLPPQLALSE 189
SmtA COG0500
SAM-dependent methyltransferase [Secondary metabolites biosynthesis, transport and catabolism, ...
 136-324 1.05e-03

SAM-dependent methyltransferase [Secondary metabolites biosynthesis, transport and catabolism, General function prediction only];


Pssm-ID: 440266 [Multi-domain]  Cd Length: 199  Bit Score: 39.51  E-value: 1.05e-03
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15236280 136 ARRVVPRVAGACQGL--FDGVATVVDVGGGTGETMGILVKEFPWiKGFNFDL-PHVIEVAQ------VLDGVENVEGDMF 206
Cdd:COG0500   7 SDELLPGLAALLALLerLPKGGRVLDLGCGTGRNLLALAARFGG-RVIGIDLsPEAIALARaraakaGLGNVEFLVADLA 85

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15236280 207 DSIPAS----DAVIIKWVLHDWGDKDCIKILKNCKEAVLPNiGKVLIVecvigekkNTMIAEERDDKlEHVRLQLDMVMM 282
Cdd:COG0500  86 ELDPLPaesfDLVVAFGVLHHLPPEEREALLRELARALKPG-GVLLLS--------ASDAAAALSLA-RLLLLATASLLE 155

                       170       180       190       200
                ....*....|....*....|....*....|....*....|..
gi 15236280 283 VHTSTGKERTLKEwDFVLTEAGFARYEVRDFDDVQSLIIAYR 324
Cdd:COG0500 156 LLLLLRLLALELY-LRALLAAAATEDLRSDALLESANALEYL 196
Methyltransf_11 pfam08241
Methyltransferase domain; Members of this family are SAM dependent methyltransferases.
 158-249 6.77e-03

Methyltransferase domain; Members of this family are SAM dependent methyltransferases.


Pssm-ID: 462406 [Multi-domain]  Cd Length: 94  Bit Score: 35.33  E-value: 6.77e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15236280   158 VDVGGGTGETMGILVKEFPWIKGFNFDlPHVIEVAQ---VLDGVENVEGDMFDsIP---AS-DAVIIKWVLHDWGDKDci 230
Cdd:pfam08241   1 LDVGCGTGLLTELLARLGARVTGVDIS-PEMLELARekaPREGLTFVVGDAED-LPfpdNSfDLVLSSEVLHHVEDPE-- 76

                          90
                  ....*....|....*....
gi 15236280   231 KILKNCKEAVLPNiGKVLI 249
Cdd:pfam08241  77 RALREIARVLKPG-GILII 94
UbiE COG2226
Ubiquinone/menaquinone biosynthesis C-methylase UbiE/MenG [Coenzyme transport and metabolism]; ...
 155-251 6.95e-03

Ubiquinone/menaquinone biosynthesis C-methylase UbiE/MenG [Coenzyme transport and metabolism]; Ubiquinone/menaquinone biosynthesis C-methylase UbiE/MenG is part of the Pathway/BioSystem: Biotin biosynthesis


Pssm-ID: 441828 [Multi-domain]  Cd Length: 143  Bit Score: 36.51  E-value: 6.95e-03
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15236280 155 ATVVDVGGGTGETMGILVKEFPWIKGFNFDlPHVIEVAQ-----VLDGVENVEGDMFDsIP---AS-DAVIIKWVLHDWG 225
Cdd:COG2226  24 ARVLDLGCGTGRLALALAERGARVTGVDIS-PEMLELAReraaeAGLNVEFVVGDAED-LPfpdGSfDLVISSFVLHHLP 101

                        90       100
                ....*....|....*....|....*.
gi 15236280 226 DKDciKILKNCKEAVLPNiGKVLIVE 251
Cdd:COG2226 102 DPE--RALAEIARVLKPG-GRLVVVD 124
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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