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Conserved domains on  [gi|15235142|ref|NP_195115|]
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transcriptional regulator of RNA polII, SAGA, subunit [Arabidopsis thaliana]

Protein Classification

TADA1 family protein( domain architecture ID 10579535)

TADA1 (transcriptional adapter 1) family protein similar to human TADA1 is a component of the STAGA transcription coactivator-HAT complex that may be involved in transcriptional regulation, and to Saccharomyces cerevisiae transcriptional coactivator HFI1/ADA1 that is a component of the transcription regulatory histone acetylation (HAT) complexes SAGA, SALSA and SLIK

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
SAGA-Tad1 pfam12767
Transcriptional regulator of RNA polII, SAGA, subunit; The yeast SAGA complex is a ...
 9-282 2.61e-33

Transcriptional regulator of RNA polII, SAGA, subunit; The yeast SAGA complex is a multifunctional coactivator that regulates transcription by RNA polymerase II. It is formed of five major modular subunits and shows a high degree of structural conservation to human TFTC and STAGA. The complex can also be conceived of as consisting of two histone-fold-containing core subunits, and this family is one of these. As a family it is likely to carry binding regions for interactions with a number of the other components of the complex.


:

Pssm-ID: 463692  Cd Length: 135  Bit Score: 119.89  E-value: 2.61e-33
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15235142     9 RLDTLEIKALIYREIGNQRAESYFNQLGRFFALKITKSEFDKLCIKTIGRQNIHLHNRLIRSIIKNAciaksppfikkgg 88
Cdd:pfam12767   1 RIDLEELKTQLKKKLGPERWEKYFEALSRFLSGKLSKAELDKLCDPILGRENLHLHNQLILSILANA------------- 67

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15235142    89 sfvrfgngdskknsqiqplhgdsafspstrkcrsrklrdrpsplgplgkphsltttneesmskaqsatellslgsrppve 168
Cdd:pfam12767     --------------------------------------------------------------------------------

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15235142   169 vvsveegeeveqiaggspsVQSRCPLTAPLGVSmslrngatrksvsnvsmcsrsfnretcqnNGELPDTRTLRSRLERRL 248
Cdd:pfam12767  68 -------------------QEIRSPYAAPLAYE-----------------------------SGELPDTDSLRKRMEPIA 99

                         250       260       270
                  ....*....|....*....|....*....|....*.
gi 15235142   249 EMEGL--KITMDSVSLLNSGLDVFMRRLIEPCLSLA 282
Cdd:pfam12767 100 YEHGLvgGVSADCANLLNNALDVYLKNLLESCIDLV 135
 
Name Accession Description Interval E-value
SAGA-Tad1 pfam12767
Transcriptional regulator of RNA polII, SAGA, subunit; The yeast SAGA complex is a ...
 9-282 2.61e-33

Transcriptional regulator of RNA polII, SAGA, subunit; The yeast SAGA complex is a multifunctional coactivator that regulates transcription by RNA polymerase II. It is formed of five major modular subunits and shows a high degree of structural conservation to human TFTC and STAGA. The complex can also be conceived of as consisting of two histone-fold-containing core subunits, and this family is one of these. As a family it is likely to carry binding regions for interactions with a number of the other components of the complex.


Pssm-ID: 463692  Cd Length: 135  Bit Score: 119.89  E-value: 2.61e-33
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15235142     9 RLDTLEIKALIYREIGNQRAESYFNQLGRFFALKITKSEFDKLCIKTIGRQNIHLHNRLIRSIIKNAciaksppfikkgg 88
Cdd:pfam12767   1 RIDLEELKTQLKKKLGPERWEKYFEALSRFLSGKLSKAELDKLCDPILGRENLHLHNQLILSILANA------------- 67

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15235142    89 sfvrfgngdskknsqiqplhgdsafspstrkcrsrklrdrpsplgplgkphsltttneesmskaqsatellslgsrppve 168
Cdd:pfam12767     --------------------------------------------------------------------------------

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15235142   169 vvsveegeeveqiaggspsVQSRCPLTAPLGVSmslrngatrksvsnvsmcsrsfnretcqnNGELPDTRTLRSRLERRL 248
Cdd:pfam12767  68 -------------------QEIRSPYAAPLAYE-----------------------------SGELPDTDSLRKRMEPIA 99

                         250       260       270
                  ....*....|....*....|....*....|....*.
gi 15235142   249 EMEGL--KITMDSVSLLNSGLDVFMRRLIEPCLSLA 282
Cdd:pfam12767 100 YEHGLvgGVSADCANLLNNALDVYLKNLLESCIDLV 135
HFD_HFI1 cd22933
histone-fold domain found in transcriptional coactivator HFI1 and similar proteins; HFI1, also ...
 227-329 2.13e-11

histone-fold domain found in transcriptional coactivator HFI1 and similar proteins; HFI1, also called ADA1, is a component of the transcription regulatory histone acetylation (HAT) complexes SAGA, SALSA and SLIK. SAGA (Spt-Ada-Gcn5-acetyltransferase) is involved in RNA polymerase II-dependent transcriptional regulation of approximately 10% of yeast genes. At the promoters, SAGA is required for recruitment of the basal transcription machinery. It influences RNA polymerase II transcriptional activity through different activities such as TATA binding protein (TBP) interaction (SPT3, SPT8 and SPT20) and promoter selectivity, interaction with transcription activators (GCN5, ADA2, ADA3 and TRA1), and chromatin modification through histone acetylation (GCN5) and deubiquitination (UBP8). SAGA acetylates nucleosomal histone H3 to some extent (to form H3K9ac, H3K14ac, H3K18ac and H3K23ac). SAGA interacts with DNA via upstream activating sequences (UASs). SALSA (SAGA altered, SPT8 absent), an altered form of SAGA, may be involved in positive transcriptional regulation. Besides lacking SPT8, SALSA contains an SPT7 subunit that is truncated. SLIK (SAGA-like) is proposed to have partly overlapping functions with SAGA. It preferentially acetylates methylated histone H3, at least after activation at the GAL1-10 locus. HFI1/ADA1 and SPT20/ADA5 may recruit TATA binding protein (TBP) and possibly other basal factors to bind to the TATA box.


Pssm-ID: 467057  Cd Length: 87  Bit Score: 59.54  E-value: 2.13e-11
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15235142 227 TCQNNGELPDTRTLRSRLER-RLEMEGLKIT-MDSVSLLNSGLDVFMRRLIEPCLSLAntrcgtdrvremnyqytqQSRR 304
Cdd:cd22933   1 LCSESKELPDADSLRERMTGiALENGLLGGVaEECVELLLLALEQHLKNILSSCIDKV------------------RARR 62

                        90       100
                ....*....|....*....|....*
gi 15235142 305 LSYVSMSDFRAGMELNTEILGEDWP 329
Cdd:cd22933  63 TSTLTLEDLLLALEINPHLLGEDPP 87
 
Name Accession Description Interval E-value
SAGA-Tad1 pfam12767
Transcriptional regulator of RNA polII, SAGA, subunit; The yeast SAGA complex is a ...
 9-282 2.61e-33

Transcriptional regulator of RNA polII, SAGA, subunit; The yeast SAGA complex is a multifunctional coactivator that regulates transcription by RNA polymerase II. It is formed of five major modular subunits and shows a high degree of structural conservation to human TFTC and STAGA. The complex can also be conceived of as consisting of two histone-fold-containing core subunits, and this family is one of these. As a family it is likely to carry binding regions for interactions with a number of the other components of the complex.


Pssm-ID: 463692  Cd Length: 135  Bit Score: 119.89  E-value: 2.61e-33
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15235142     9 RLDTLEIKALIYREIGNQRAESYFNQLGRFFALKITKSEFDKLCIKTIGRQNIHLHNRLIRSIIKNAciaksppfikkgg 88
Cdd:pfam12767   1 RIDLEELKTQLKKKLGPERWEKYFEALSRFLSGKLSKAELDKLCDPILGRENLHLHNQLILSILANA------------- 67

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15235142    89 sfvrfgngdskknsqiqplhgdsafspstrkcrsrklrdrpsplgplgkphsltttneesmskaqsatellslgsrppve 168
Cdd:pfam12767     --------------------------------------------------------------------------------

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15235142   169 vvsveegeeveqiaggspsVQSRCPLTAPLGVSmslrngatrksvsnvsmcsrsfnretcqnNGELPDTRTLRSRLERRL 248
Cdd:pfam12767  68 -------------------QEIRSPYAAPLAYE-----------------------------SGELPDTDSLRKRMEPIA 99

                         250       260       270
                  ....*....|....*....|....*....|....*.
gi 15235142   249 EMEGL--KITMDSVSLLNSGLDVFMRRLIEPCLSLA 282
Cdd:pfam12767 100 YEHGLvgGVSADCANLLNNALDVYLKNLLESCIDLV 135
HFD_HFI1 cd22933
histone-fold domain found in transcriptional coactivator HFI1 and similar proteins; HFI1, also ...
 227-329 2.13e-11

histone-fold domain found in transcriptional coactivator HFI1 and similar proteins; HFI1, also called ADA1, is a component of the transcription regulatory histone acetylation (HAT) complexes SAGA, SALSA and SLIK. SAGA (Spt-Ada-Gcn5-acetyltransferase) is involved in RNA polymerase II-dependent transcriptional regulation of approximately 10% of yeast genes. At the promoters, SAGA is required for recruitment of the basal transcription machinery. It influences RNA polymerase II transcriptional activity through different activities such as TATA binding protein (TBP) interaction (SPT3, SPT8 and SPT20) and promoter selectivity, interaction with transcription activators (GCN5, ADA2, ADA3 and TRA1), and chromatin modification through histone acetylation (GCN5) and deubiquitination (UBP8). SAGA acetylates nucleosomal histone H3 to some extent (to form H3K9ac, H3K14ac, H3K18ac and H3K23ac). SAGA interacts with DNA via upstream activating sequences (UASs). SALSA (SAGA altered, SPT8 absent), an altered form of SAGA, may be involved in positive transcriptional regulation. Besides lacking SPT8, SALSA contains an SPT7 subunit that is truncated. SLIK (SAGA-like) is proposed to have partly overlapping functions with SAGA. It preferentially acetylates methylated histone H3, at least after activation at the GAL1-10 locus. HFI1/ADA1 and SPT20/ADA5 may recruit TATA binding protein (TBP) and possibly other basal factors to bind to the TATA box.


Pssm-ID: 467057  Cd Length: 87  Bit Score: 59.54  E-value: 2.13e-11
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15235142 227 TCQNNGELPDTRTLRSRLER-RLEMEGLKIT-MDSVSLLNSGLDVFMRRLIEPCLSLAntrcgtdrvremnyqytqQSRR 304
Cdd:cd22933   1 LCSESKELPDADSLRERMTGiALENGLLGGVaEECVELLLLALEQHLKNILSSCIDKV------------------RARR 62

                        90       100
                ....*....|....*....|....*
gi 15235142 305 LSYVSMSDFRAGMELNTEILGEDWP 329
Cdd:cd22933  63 TSTLTLEDLLLALEINPHLLGEDPP 87
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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