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Conserved domains on  [gi|15236028|ref|NP_194891|]
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Transcriptional factor B3 family protein [Arabidopsis thaliana]

Protein Classification

B3 domain-containing protein( domain architecture ID 10659976)

B3 DNA-binding domain-containing protein such as Arabidopsis thaliana REM1 that may play a role in flower development

Graphical summary

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List of domain hits

Name Accession Description Interval E-value
B3 smart01019
B3 DNA binding domain; Two DNA binding proteins, RAV1 and RAV2 from Arabidopsis thaliana ...
169-265 2.75e-25

B3 DNA binding domain; Two DNA binding proteins, RAV1 and RAV2 from Arabidopsis thaliana contain two distinct amino acid sequence domains found only in higher plant species. The N-terminal regions of RAV1 and RAV2 are homologous to the AP2 DNA-binding domain (see ) present in a family of transcription factors, while the C-terminal region exhibits homology to the highly conserved C-terminal domain, designated B3, of VP1/ABI3 transcription factors. The AP2 and B3-like domains of RAV1 bind autonomously to the CAACA and CACCTG motifs, respectively, and together achieve a high affinity and specificity of binding. It has been suggested that the AP2 and B3-like domains of RAV1 are connected by a highly flexible structure enabling the two domains to bind to the CAACA and CACCTG motifs in various spacings and orientations.


:

Pssm-ID: 214977  Cd Length: 96  Bit Score: 99.68  E-value: 2.75e-25
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15236028    169 YLVAHVTPSSLLRDNMCVLSKFARSNGLDRRECEIDLRDEHEKSWTLLLRHNKKTGQAFMRGGWRSFCRNNGIKAGSICR 248
Cdd:smart01019   1 FFKTLTTSDVSKHGRLVLPKQFAESNGPPLKPKEITLLDPDGKSWKVKLKYRGSSGMFLLTGGWKEFVKANGLKAGDSLT 80
                           90
                   ....*....|....*..
gi 15236028    249 FKLVQSGIkPVLQLCPN 265
Cdd:smart01019  81 FELDGNGE-FVVSIFRK 96
B3 smart01019
B3 DNA binding domain; Two DNA binding proteins, RAV1 and RAV2 from Arabidopsis thaliana ...
307-402 1.38e-15

B3 DNA binding domain; Two DNA binding proteins, RAV1 and RAV2 from Arabidopsis thaliana contain two distinct amino acid sequence domains found only in higher plant species. The N-terminal regions of RAV1 and RAV2 are homologous to the AP2 DNA-binding domain (see ) present in a family of transcription factors, while the C-terminal region exhibits homology to the highly conserved C-terminal domain, designated B3, of VP1/ABI3 transcription factors. The AP2 and B3-like domains of RAV1 bind autonomously to the CAACA and CACCTG motifs, respectively, and together achieve a high affinity and specificity of binding. It has been suggested that the AP2 and B3-like domains of RAV1 are connected by a highly flexible structure enabling the two domains to bind to the CAACA and CACCTG motifs in various spacings and orientations.


:

Pssm-ID: 214977  Cd Length: 96  Bit Score: 72.33  E-value: 1.38e-15
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15236028    307 ILTFDLKPYVFRSCQFFLPASFARENGIVE-AGEVTVLNKDGIEWKSHLVNIKGRDQFY-NRGCQDFFVANGVKnVGDPF 384
Cdd:smart01019   1 FFKTLTTSDVSKHGRLVLPKQFAESNGPPLkPKEITLLDPDGKSWKVKLKYRGSSGMFLlTGGWKEFVKANGLK-AGDSL 79
                           90
                   ....*....|....*...
gi 15236028    385 TLEVIRGGpSPILKICSK 402
Cdd:smart01019  80 TFELDGNG-EFVVSIFRK 96
B3 smart01019
B3 DNA binding domain; Two DNA binding proteins, RAV1 and RAV2 from Arabidopsis thaliana ...
14-102 9.71e-13

B3 DNA binding domain; Two DNA binding proteins, RAV1 and RAV2 from Arabidopsis thaliana contain two distinct amino acid sequence domains found only in higher plant species. The N-terminal regions of RAV1 and RAV2 are homologous to the AP2 DNA-binding domain (see ) present in a family of transcription factors, while the C-terminal region exhibits homology to the highly conserved C-terminal domain, designated B3, of VP1/ABI3 transcription factors. The AP2 and B3-like domains of RAV1 bind autonomously to the CAACA and CACCTG motifs, respectively, and together achieve a high affinity and specificity of binding. It has been suggested that the AP2 and B3-like domains of RAV1 are connected by a highly flexible structure enabling the two domains to bind to the CAACA and CACCTG motifs in various spacings and orientations.


:

Pssm-ID: 214977  Cd Length: 96  Bit Score: 64.24  E-value: 9.71e-13
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15236028     14 FFIIDL---SGQKSNPIIPTEFIWNHFNGKIQSTnMKLTsDASDRNWDVKLDGAR------FAGGWKDFSVSHSVRDDDL 84
Cdd:smart01019   1 FFKTLTtsdVSKHGRLVLPKQFAESNGPPLKPKE-ITLL-DPDGKSWKVKLKYRGssgmflLTGGWKEFVKANGLKAGDS 78
                           90
                   ....*....|....*...
gi 15236028     85 LSFRHDGGMVFHVSPFGR 102
Cdd:smart01019  79 LTFELDGNGEFVVSIFRK 96
 
Name Accession Description Interval E-value
B3 smart01019
B3 DNA binding domain; Two DNA binding proteins, RAV1 and RAV2 from Arabidopsis thaliana ...
169-265 2.75e-25

B3 DNA binding domain; Two DNA binding proteins, RAV1 and RAV2 from Arabidopsis thaliana contain two distinct amino acid sequence domains found only in higher plant species. The N-terminal regions of RAV1 and RAV2 are homologous to the AP2 DNA-binding domain (see ) present in a family of transcription factors, while the C-terminal region exhibits homology to the highly conserved C-terminal domain, designated B3, of VP1/ABI3 transcription factors. The AP2 and B3-like domains of RAV1 bind autonomously to the CAACA and CACCTG motifs, respectively, and together achieve a high affinity and specificity of binding. It has been suggested that the AP2 and B3-like domains of RAV1 are connected by a highly flexible structure enabling the two domains to bind to the CAACA and CACCTG motifs in various spacings and orientations.


Pssm-ID: 214977  Cd Length: 96  Bit Score: 99.68  E-value: 2.75e-25
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15236028    169 YLVAHVTPSSLLRDNMCVLSKFARSNGLDRRECEIDLRDEHEKSWTLLLRHNKKTGQAFMRGGWRSFCRNNGIKAGSICR 248
Cdd:smart01019   1 FFKTLTTSDVSKHGRLVLPKQFAESNGPPLKPKEITLLDPDGKSWKVKLKYRGSSGMFLLTGGWKEFVKANGLKAGDSLT 80
                           90
                   ....*....|....*..
gi 15236028    249 FKLVQSGIkPVLQLCPN 265
Cdd:smart01019  81 FELDGNGE-FVVSIFRK 96
B3 pfam02362
B3 DNA binding domain; This is a family of plant transcription factors with various roles in ...
169-265 1.01e-22

B3 DNA binding domain; This is a family of plant transcription factors with various roles in development, the aligned region corresponds the B3 DNA binding domain as described in this domain is found in VP1/AB13 transcription factors. Some proteins also have a second AP2 DNA binding domain pfam00847 such as RAV1. DNA binding activity was demonstrated by.


Pssm-ID: 396783 [Multi-domain]  Cd Length: 101  Bit Score: 92.44  E-value: 1.01e-22
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15236028   169 YLVAHVTPSSLLRDNMCVLSKFARSN-GLDRREC-EIDLRDEHEKSWTLLLRHNKKTGQAFMRGGWRSFCRNNGIKAGSI 246
Cdd:pfam02362   1 FFKVLVTSDVLSSGRLVIPKKFARENsGLPKRLSkEITLLDEHGKSWTVKLRYRKKSGRYVLTGGWKEFVKANGLKAGDF 80
                          90       100
                  ....*....|....*....|
gi 15236028   247 CRFKLVQ-SGIKPVLQLCPN 265
Cdd:pfam02362  81 LTFKLVGnSGFTPVVGIFRK 100
B3_DNA cd10017
Plant-specific B3-DNA binding domain; The plant-specific B3 DNA binding domain superfamily ...
169-264 3.09e-20

Plant-specific B3-DNA binding domain; The plant-specific B3 DNA binding domain superfamily includes the well-characterized auxin response factor (ARF) and the LAV (Leafy cotyledon2 [LEC2]-Abscisic acid insensitive3 [ABI3]-VAL) families, as well as the RAV (Related to ABI3 and VP1) and REM (REproductive Meristem) families. LEC2 and ABI3 have been shown to be involved in seed development, while other members of the LAV family seem to have a more general role, being expressed in many organs during plant development. Members of the ARF family bind to the auxin response element and depending on presence of an activation or repression domain, they activate or repress transcription. RAV and REM families are less studied B3 protein famillies.


Pssm-ID: 197383  Cd Length: 98  Bit Score: 85.46  E-value: 3.09e-20
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15236028 169 YLVAHVTPSSLLRDNMCVLSK-FARSNGLDRRECEIDLRDEHEKSWTLLLRHNKKTGQAFMRGGWRSFCRNNGIKAGSIC 247
Cdd:cd10017   2 LFFKVLTPSDVSSLGRLVLPKkFAKAHLPPKEGVEVTLEDPDGKKWTVKLKKRKNSGRMVLSGGWKEFVRANGLKEGDFL 81
                        90
                ....*....|....*..
gi 15236028 248 RFKLVQSGIKPVLQLCP 264
Cdd:cd10017  82 VFELDGRNSKFKVRIFR 98
B3 smart01019
B3 DNA binding domain; Two DNA binding proteins, RAV1 and RAV2 from Arabidopsis thaliana ...
307-402 1.38e-15

B3 DNA binding domain; Two DNA binding proteins, RAV1 and RAV2 from Arabidopsis thaliana contain two distinct amino acid sequence domains found only in higher plant species. The N-terminal regions of RAV1 and RAV2 are homologous to the AP2 DNA-binding domain (see ) present in a family of transcription factors, while the C-terminal region exhibits homology to the highly conserved C-terminal domain, designated B3, of VP1/ABI3 transcription factors. The AP2 and B3-like domains of RAV1 bind autonomously to the CAACA and CACCTG motifs, respectively, and together achieve a high affinity and specificity of binding. It has been suggested that the AP2 and B3-like domains of RAV1 are connected by a highly flexible structure enabling the two domains to bind to the CAACA and CACCTG motifs in various spacings and orientations.


Pssm-ID: 214977  Cd Length: 96  Bit Score: 72.33  E-value: 1.38e-15
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15236028    307 ILTFDLKPYVFRSCQFFLPASFARENGIVE-AGEVTVLNKDGIEWKSHLVNIKGRDQFY-NRGCQDFFVANGVKnVGDPF 384
Cdd:smart01019   1 FFKTLTTSDVSKHGRLVLPKQFAESNGPPLkPKEITLLDPDGKSWKVKLKYRGSSGMFLlTGGWKEFVKANGLK-AGDSL 79
                           90
                   ....*....|....*...
gi 15236028    385 TLEVIRGGpSPILKICSK 402
Cdd:smart01019  80 TFELDGNG-EFVVSIFRK 96
B3 pfam02362
B3 DNA binding domain; This is a family of plant transcription factors with various roles in ...
308-403 1.85e-14

B3 DNA binding domain; This is a family of plant transcription factors with various roles in development, the aligned region corresponds the B3 DNA binding domain as described in this domain is found in VP1/AB13 transcription factors. Some proteins also have a second AP2 DNA binding domain pfam00847 such as RAV1. DNA binding activity was demonstrated by.


Pssm-ID: 396783 [Multi-domain]  Cd Length: 101  Bit Score: 69.32  E-value: 1.85e-14
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15236028   308 LTFDLKPYVFRSCQFFLPASFARENG---IVEAGEVTVLNKDGIEWKSHLVNIKGRDQFY-NRGCQDFFVANGVKnVGDP 383
Cdd:pfam02362   2 FKVLVTSDVLSSGRLVIPKKFARENSglpKRLSKEITLLDEHGKSWTVKLRYRKKSGRYVlTGGWKEFVKANGLK-AGDF 80
                          90       100
                  ....*....|....*....|.
gi 15236028   384 FTLEVIRGGP-SPILKICSKV 403
Cdd:pfam02362  81 LTFKLVGNSGfTPVVGIFRKS 101
B3 smart01019
B3 DNA binding domain; Two DNA binding proteins, RAV1 and RAV2 from Arabidopsis thaliana ...
14-102 9.71e-13

B3 DNA binding domain; Two DNA binding proteins, RAV1 and RAV2 from Arabidopsis thaliana contain two distinct amino acid sequence domains found only in higher plant species. The N-terminal regions of RAV1 and RAV2 are homologous to the AP2 DNA-binding domain (see ) present in a family of transcription factors, while the C-terminal region exhibits homology to the highly conserved C-terminal domain, designated B3, of VP1/ABI3 transcription factors. The AP2 and B3-like domains of RAV1 bind autonomously to the CAACA and CACCTG motifs, respectively, and together achieve a high affinity and specificity of binding. It has been suggested that the AP2 and B3-like domains of RAV1 are connected by a highly flexible structure enabling the two domains to bind to the CAACA and CACCTG motifs in various spacings and orientations.


Pssm-ID: 214977  Cd Length: 96  Bit Score: 64.24  E-value: 9.71e-13
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15236028     14 FFIIDL---SGQKSNPIIPTEFIWNHFNGKIQSTnMKLTsDASDRNWDVKLDGAR------FAGGWKDFSVSHSVRDDDL 84
Cdd:smart01019   1 FFKTLTtsdVSKHGRLVLPKQFAESNGPPLKPKE-ITLL-DPDGKSWKVKLKYRGssgmflLTGGWKEFVKANGLKAGDS 78
                           90
                   ....*....|....*...
gi 15236028     85 LSFRHDGGMVFHVSPFGR 102
Cdd:smart01019  79 LTFELDGNGEFVVSIFRK 96
B3_DNA cd10017
Plant-specific B3-DNA binding domain; The plant-specific B3 DNA binding domain superfamily ...
27-100 9.68e-12

Plant-specific B3-DNA binding domain; The plant-specific B3 DNA binding domain superfamily includes the well-characterized auxin response factor (ARF) and the LAV (Leafy cotyledon2 [LEC2]-Abscisic acid insensitive3 [ABI3]-VAL) families, as well as the RAV (Related to ABI3 and VP1) and REM (REproductive Meristem) families. LEC2 and ABI3 have been shown to be involved in seed development, while other members of the LAV family seem to have a more general role, being expressed in many organs during plant development. Members of the ARF family bind to the auxin response element and depending on presence of an activation or repression domain, they activate or repress transcription. RAV and REM families are less studied B3 protein famillies.


Pssm-ID: 197383  Cd Length: 98  Bit Score: 61.19  E-value: 9.68e-12
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15236028  27 IIPTEFIWNHFNGKIqSTNMKLTsDASDRNWDVKL------DGARFAGGWKDFSVSHSVRDDDLLSFRHDG-GMVFHVSP 99
Cdd:cd10017  19 VLPKKFAKAHLPPKE-GVEVTLE-DPDGKKWTVKLkkrknsGRMVLSGGWKEFVRANGLKEGDFLVFELDGrNSKFKVRI 96

                .
gi 15236028 100 F 100
Cdd:cd10017  97 F 97
B3_DNA cd10017
Plant-specific B3-DNA binding domain; The plant-specific B3 DNA binding domain superfamily ...
306-401 2.07e-08

Plant-specific B3-DNA binding domain; The plant-specific B3 DNA binding domain superfamily includes the well-characterized auxin response factor (ARF) and the LAV (Leafy cotyledon2 [LEC2]-Abscisic acid insensitive3 [ABI3]-VAL) families, as well as the RAV (Related to ABI3 and VP1) and REM (REproductive Meristem) families. LEC2 and ABI3 have been shown to be involved in seed development, while other members of the LAV family seem to have a more general role, being expressed in many organs during plant development. Members of the ARF family bind to the auxin response element and depending on presence of an activation or repression domain, they activate or repress transcription. RAV and REM families are less studied B3 protein famillies.


Pssm-ID: 197383  Cd Length: 98  Bit Score: 51.94  E-value: 2.07e-08
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15236028 306 KILTfdlKPYVFRSCQFFLPASFARENGI-VEAGEVTVLNKDGIEWKSHLVNIKGRDQFY-NRGCQDFFVANGVKnVGDP 383
Cdd:cd10017   5 KVLT---PSDVSSLGRLVLPKKFAKAHLPpKEGVEVTLEDPDGKKWTVKLKKRKNSGRMVlSGGWKEFVRANGLK-EGDF 80
                        90
                ....*....|....*...
gi 15236028 384 FTLEVIRGGPSPILKICS 401
Cdd:cd10017  81 LVFELDGRNSKFKVRIFR 98
B3 pfam02362
B3 DNA binding domain; This is a family of plant transcription factors with various roles in ...
27-95 3.97e-03

B3 DNA binding domain; This is a family of plant transcription factors with various roles in development, the aligned region corresponds the B3 DNA binding domain as described in this domain is found in VP1/AB13 transcription factors. Some proteins also have a second AP2 DNA binding domain pfam00847 such as RAV1. DNA binding activity was demonstrated by.


Pssm-ID: 396783 [Multi-domain]  Cd Length: 101  Bit Score: 36.97  E-value: 3.97e-03
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 15236028    27 IIPTEFIWNHFNG-KIQSTNMKLTsDASDRNWDVKL------DGARFAGGWKDFSVSHSVRDDDLLSFRHDGGMVF 95
Cdd:pfam02362  17 VIPKKFARENSGLpKRLSKEITLL-DEHGKSWTVKLryrkksGRYVLTGGWKEFVKANGLKAGDFLTFKLVGNSGF 91
 
Name Accession Description Interval E-value
B3 smart01019
B3 DNA binding domain; Two DNA binding proteins, RAV1 and RAV2 from Arabidopsis thaliana ...
169-265 2.75e-25

B3 DNA binding domain; Two DNA binding proteins, RAV1 and RAV2 from Arabidopsis thaliana contain two distinct amino acid sequence domains found only in higher plant species. The N-terminal regions of RAV1 and RAV2 are homologous to the AP2 DNA-binding domain (see ) present in a family of transcription factors, while the C-terminal region exhibits homology to the highly conserved C-terminal domain, designated B3, of VP1/ABI3 transcription factors. The AP2 and B3-like domains of RAV1 bind autonomously to the CAACA and CACCTG motifs, respectively, and together achieve a high affinity and specificity of binding. It has been suggested that the AP2 and B3-like domains of RAV1 are connected by a highly flexible structure enabling the two domains to bind to the CAACA and CACCTG motifs in various spacings and orientations.


Pssm-ID: 214977  Cd Length: 96  Bit Score: 99.68  E-value: 2.75e-25
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15236028    169 YLVAHVTPSSLLRDNMCVLSKFARSNGLDRRECEIDLRDEHEKSWTLLLRHNKKTGQAFMRGGWRSFCRNNGIKAGSICR 248
Cdd:smart01019   1 FFKTLTTSDVSKHGRLVLPKQFAESNGPPLKPKEITLLDPDGKSWKVKLKYRGSSGMFLLTGGWKEFVKANGLKAGDSLT 80
                           90
                   ....*....|....*..
gi 15236028    249 FKLVQSGIkPVLQLCPN 265
Cdd:smart01019  81 FELDGNGE-FVVSIFRK 96
B3 pfam02362
B3 DNA binding domain; This is a family of plant transcription factors with various roles in ...
169-265 1.01e-22

B3 DNA binding domain; This is a family of plant transcription factors with various roles in development, the aligned region corresponds the B3 DNA binding domain as described in this domain is found in VP1/AB13 transcription factors. Some proteins also have a second AP2 DNA binding domain pfam00847 such as RAV1. DNA binding activity was demonstrated by.


Pssm-ID: 396783 [Multi-domain]  Cd Length: 101  Bit Score: 92.44  E-value: 1.01e-22
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15236028   169 YLVAHVTPSSLLRDNMCVLSKFARSN-GLDRREC-EIDLRDEHEKSWTLLLRHNKKTGQAFMRGGWRSFCRNNGIKAGSI 246
Cdd:pfam02362   1 FFKVLVTSDVLSSGRLVIPKKFARENsGLPKRLSkEITLLDEHGKSWTVKLRYRKKSGRYVLTGGWKEFVKANGLKAGDF 80
                          90       100
                  ....*....|....*....|
gi 15236028   247 CRFKLVQ-SGIKPVLQLCPN 265
Cdd:pfam02362  81 LTFKLVGnSGFTPVVGIFRK 100
B3_DNA cd10017
Plant-specific B3-DNA binding domain; The plant-specific B3 DNA binding domain superfamily ...
169-264 3.09e-20

Plant-specific B3-DNA binding domain; The plant-specific B3 DNA binding domain superfamily includes the well-characterized auxin response factor (ARF) and the LAV (Leafy cotyledon2 [LEC2]-Abscisic acid insensitive3 [ABI3]-VAL) families, as well as the RAV (Related to ABI3 and VP1) and REM (REproductive Meristem) families. LEC2 and ABI3 have been shown to be involved in seed development, while other members of the LAV family seem to have a more general role, being expressed in many organs during plant development. Members of the ARF family bind to the auxin response element and depending on presence of an activation or repression domain, they activate or repress transcription. RAV and REM families are less studied B3 protein famillies.


Pssm-ID: 197383  Cd Length: 98  Bit Score: 85.46  E-value: 3.09e-20
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15236028 169 YLVAHVTPSSLLRDNMCVLSK-FARSNGLDRRECEIDLRDEHEKSWTLLLRHNKKTGQAFMRGGWRSFCRNNGIKAGSIC 247
Cdd:cd10017   2 LFFKVLTPSDVSSLGRLVLPKkFAKAHLPPKEGVEVTLEDPDGKKWTVKLKKRKNSGRMVLSGGWKEFVRANGLKEGDFL 81
                        90
                ....*....|....*..
gi 15236028 248 RFKLVQSGIKPVLQLCP 264
Cdd:cd10017  82 VFELDGRNSKFKVRIFR 98
B3 smart01019
B3 DNA binding domain; Two DNA binding proteins, RAV1 and RAV2 from Arabidopsis thaliana ...
307-402 1.38e-15

B3 DNA binding domain; Two DNA binding proteins, RAV1 and RAV2 from Arabidopsis thaliana contain two distinct amino acid sequence domains found only in higher plant species. The N-terminal regions of RAV1 and RAV2 are homologous to the AP2 DNA-binding domain (see ) present in a family of transcription factors, while the C-terminal region exhibits homology to the highly conserved C-terminal domain, designated B3, of VP1/ABI3 transcription factors. The AP2 and B3-like domains of RAV1 bind autonomously to the CAACA and CACCTG motifs, respectively, and together achieve a high affinity and specificity of binding. It has been suggested that the AP2 and B3-like domains of RAV1 are connected by a highly flexible structure enabling the two domains to bind to the CAACA and CACCTG motifs in various spacings and orientations.


Pssm-ID: 214977  Cd Length: 96  Bit Score: 72.33  E-value: 1.38e-15
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15236028    307 ILTFDLKPYVFRSCQFFLPASFARENGIVE-AGEVTVLNKDGIEWKSHLVNIKGRDQFY-NRGCQDFFVANGVKnVGDPF 384
Cdd:smart01019   1 FFKTLTTSDVSKHGRLVLPKQFAESNGPPLkPKEITLLDPDGKSWKVKLKYRGSSGMFLlTGGWKEFVKANGLK-AGDSL 79
                           90
                   ....*....|....*...
gi 15236028    385 TLEVIRGGpSPILKICSK 402
Cdd:smart01019  80 TFELDGNG-EFVVSIFRK 96
B3 pfam02362
B3 DNA binding domain; This is a family of plant transcription factors with various roles in ...
308-403 1.85e-14

B3 DNA binding domain; This is a family of plant transcription factors with various roles in development, the aligned region corresponds the B3 DNA binding domain as described in this domain is found in VP1/AB13 transcription factors. Some proteins also have a second AP2 DNA binding domain pfam00847 such as RAV1. DNA binding activity was demonstrated by.


Pssm-ID: 396783 [Multi-domain]  Cd Length: 101  Bit Score: 69.32  E-value: 1.85e-14
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15236028   308 LTFDLKPYVFRSCQFFLPASFARENG---IVEAGEVTVLNKDGIEWKSHLVNIKGRDQFY-NRGCQDFFVANGVKnVGDP 383
Cdd:pfam02362   2 FKVLVTSDVLSSGRLVIPKKFARENSglpKRLSKEITLLDEHGKSWTVKLRYRKKSGRYVlTGGWKEFVKANGLK-AGDF 80
                          90       100
                  ....*....|....*....|.
gi 15236028   384 FTLEVIRGGP-SPILKICSKV 403
Cdd:pfam02362  81 LTFKLVGNSGfTPVVGIFRKS 101
B3 smart01019
B3 DNA binding domain; Two DNA binding proteins, RAV1 and RAV2 from Arabidopsis thaliana ...
14-102 9.71e-13

B3 DNA binding domain; Two DNA binding proteins, RAV1 and RAV2 from Arabidopsis thaliana contain two distinct amino acid sequence domains found only in higher plant species. The N-terminal regions of RAV1 and RAV2 are homologous to the AP2 DNA-binding domain (see ) present in a family of transcription factors, while the C-terminal region exhibits homology to the highly conserved C-terminal domain, designated B3, of VP1/ABI3 transcription factors. The AP2 and B3-like domains of RAV1 bind autonomously to the CAACA and CACCTG motifs, respectively, and together achieve a high affinity and specificity of binding. It has been suggested that the AP2 and B3-like domains of RAV1 are connected by a highly flexible structure enabling the two domains to bind to the CAACA and CACCTG motifs in various spacings and orientations.


Pssm-ID: 214977  Cd Length: 96  Bit Score: 64.24  E-value: 9.71e-13
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15236028     14 FFIIDL---SGQKSNPIIPTEFIWNHFNGKIQSTnMKLTsDASDRNWDVKLDGAR------FAGGWKDFSVSHSVRDDDL 84
Cdd:smart01019   1 FFKTLTtsdVSKHGRLVLPKQFAESNGPPLKPKE-ITLL-DPDGKSWKVKLKYRGssgmflLTGGWKEFVKANGLKAGDS 78
                           90
                   ....*....|....*...
gi 15236028     85 LSFRHDGGMVFHVSPFGR 102
Cdd:smart01019  79 LTFELDGNGEFVVSIFRK 96
B3_DNA cd10017
Plant-specific B3-DNA binding domain; The plant-specific B3 DNA binding domain superfamily ...
27-100 9.68e-12

Plant-specific B3-DNA binding domain; The plant-specific B3 DNA binding domain superfamily includes the well-characterized auxin response factor (ARF) and the LAV (Leafy cotyledon2 [LEC2]-Abscisic acid insensitive3 [ABI3]-VAL) families, as well as the RAV (Related to ABI3 and VP1) and REM (REproductive Meristem) families. LEC2 and ABI3 have been shown to be involved in seed development, while other members of the LAV family seem to have a more general role, being expressed in many organs during plant development. Members of the ARF family bind to the auxin response element and depending on presence of an activation or repression domain, they activate or repress transcription. RAV and REM families are less studied B3 protein famillies.


Pssm-ID: 197383  Cd Length: 98  Bit Score: 61.19  E-value: 9.68e-12
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15236028  27 IIPTEFIWNHFNGKIqSTNMKLTsDASDRNWDVKL------DGARFAGGWKDFSVSHSVRDDDLLSFRHDG-GMVFHVSP 99
Cdd:cd10017  19 VLPKKFAKAHLPPKE-GVEVTLE-DPDGKKWTVKLkkrknsGRMVLSGGWKEFVRANGLKEGDFLVFELDGrNSKFKVRI 96

                .
gi 15236028 100 F 100
Cdd:cd10017  97 F 97
B3_DNA cd10017
Plant-specific B3-DNA binding domain; The plant-specific B3 DNA binding domain superfamily ...
306-401 2.07e-08

Plant-specific B3-DNA binding domain; The plant-specific B3 DNA binding domain superfamily includes the well-characterized auxin response factor (ARF) and the LAV (Leafy cotyledon2 [LEC2]-Abscisic acid insensitive3 [ABI3]-VAL) families, as well as the RAV (Related to ABI3 and VP1) and REM (REproductive Meristem) families. LEC2 and ABI3 have been shown to be involved in seed development, while other members of the LAV family seem to have a more general role, being expressed in many organs during plant development. Members of the ARF family bind to the auxin response element and depending on presence of an activation or repression domain, they activate or repress transcription. RAV and REM families are less studied B3 protein famillies.


Pssm-ID: 197383  Cd Length: 98  Bit Score: 51.94  E-value: 2.07e-08
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15236028 306 KILTfdlKPYVFRSCQFFLPASFARENGI-VEAGEVTVLNKDGIEWKSHLVNIKGRDQFY-NRGCQDFFVANGVKnVGDP 383
Cdd:cd10017   5 KVLT---PSDVSSLGRLVLPKKFAKAHLPpKEGVEVTLEDPDGKKWTVKLKKRKNSGRMVlSGGWKEFVRANGLK-EGDF 80
                        90
                ....*....|....*...
gi 15236028 384 FTLEVIRGGPSPILKICS 401
Cdd:cd10017  81 LVFELDGRNSKFKVRIFR 98
B3 pfam02362
B3 DNA binding domain; This is a family of plant transcription factors with various roles in ...
27-95 3.97e-03

B3 DNA binding domain; This is a family of plant transcription factors with various roles in development, the aligned region corresponds the B3 DNA binding domain as described in this domain is found in VP1/AB13 transcription factors. Some proteins also have a second AP2 DNA binding domain pfam00847 such as RAV1. DNA binding activity was demonstrated by.


Pssm-ID: 396783 [Multi-domain]  Cd Length: 101  Bit Score: 36.97  E-value: 3.97e-03
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 15236028    27 IIPTEFIWNHFNG-KIQSTNMKLTsDASDRNWDVKL------DGARFAGGWKDFSVSHSVRDDDLLSFRHDGGMVF 95
Cdd:pfam02362  17 VIPKKFARENSGLpKRLSKEITLL-DEHGKSWTVKLryrkksGRYVLTGGWKEFVKANGLKAGDFLTFKLVGNSGF 91
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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