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Conserved domains on  [gi|30688025|ref|NP_194587|]
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magnesium transport 5 [Arabidopsis thaliana]

Protein Classification

CorA family magnesium transporter( domain architecture ID 10191269)

CorA family magnesium transporter, belonging to the CorA metal ion transporter (MIT) family, mediates the influx of magnesium ions, similar to mitochondrial inner membrane magnesium transporter MRS2

CATH:  1.20.58.340
Gene Ontology:  GO:0015095|GO:1903830|GO:0016020
PubMed:  17619822|18927203|16720382
SCOP:  4000404|4000464
TCDB:  1.A.35

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
Mrs2_Mfm1p-like cd12823
Saccharomyces cerevisiae inner mitochondrial membrane Mg2+ transporters Mfm1p and Mrs2p-like ...
 55-406 2.08e-107

Saccharomyces cerevisiae inner mitochondrial membrane Mg2+ transporters Mfm1p and Mrs2p-like family; A eukaryotic subfamily belonging to the Escherichia coli CorA-Salmonella typhimurium ZntB_like family (EcCorA_ZntB-like) family of the MIT superfamily of essential membrane proteins involved in transporting divalent cations (uptake or efflux) across membranes. This functionally diverse subfamily includes the inner mitochondrial membrane Mg2+ transporters Saccharomyces cerevisiae Mfm1p/Lpe10p, Mrs2p, and human MRS2/ MRS2L. It also includes a family of Arabidopsis thaliana proteins (AtMGTs) some of which are localized to distinct tissues, and not all of which can transport Mg2+. Structures of the intracellular domain of two EcCorA_ZntB-like family transporters: Vibrio parahaemolyticus and Salmonella typhimurium ZntB form funnel-shaped homopentamers, the tip of the funnel is formed from two C-terminal transmembrane (TM) helices from each monomer, and the large opening of the funnel from the N-terminal cytoplasmic domains. The GMN signature motif of the MIT superfamily occurs just after TM1, mutation within this motif is known to abolish Mg2+ transport through Salmonella typhimurium CorA, and Mrs2p. Natural variants such as GVN and GIN, as in some ZntB family proteins, may be associated with the transport of different divalent cations, such as zinc and cadmium. The functional diversity of MIT transporters may also be due to minor structural differences regulating gating, substrate selection, and transport.


:

Pssm-ID: 213357  Cd Length: 323  Bit Score: 319.19  E-value: 2.08e-107
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 30688025  55 WTRFDRTGFMEVAGCDKSTIIERSSVSAKDLR----TAFSHSSKILAREKAIVLNLEVIKAVITSEQVMLLDSLR---PE 127
Cdd:cd12823   1 WTVIDSTGNATVVELDKSELLRELGLQPRDLRlldpTLTSYPPSILVRENAILVNLEHIRAIITADEVLLFDPDGsssAL 80

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 30688025 128 VLTLTDRLKHHFPRKDGPEnilqasshghqegGEEGLKSKLPFEFRVLEIAFEVFCSFVDSNVVDLETQAWSILDELTKK 207
Cdd:cd12823  81 VSAFLEELQRRLASSNGSE-------------SESGGEDSLPFEFRALEAALEEVCSHLEAELKRLEPEALPLLDELTDK 147

                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 30688025 208 VSNENLKDLRSLKTSLTHLLARVQKVRDEIEHFLDDKEDMEDLYLTRKWIQNQQTEAAsnsivsqpnlqrhtsnristsm 287
Cdd:cd12823 148 ISTSNLERLLPLKRRLVELETRVQKVRDALEELLDDDEDMADMYLTDKAAGPERLESS---------------------- 205

                       250       260       270       280       290       300       310       320
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 30688025 288 vTEEDDIDDMEMLLEAYFMQLEGMRNKILLMKEHIDSTEAYVKILQNSRRNGLIHLMMLVNIGNYAITAGTVVVNLFGMN 367
Cdd:cd12823 206 -RKEDDHEEVEMLLEAYLQQVDELLNKLEELREYIDDTEELINLILDSRRNQLMRLELKLSIGTLSLAVGTLVAGLFGMN 284

                       330       340       350
                ....*....|....*....|....*....|....*....
gi 30688025 368 IPIGLYSTPDIFGYVVWAVVALCIVLFIVTVGYAKWKKL 406
Cdd:cd12823 285 LPSGLEESPYAFWIVTGGSVVGSILIFIVVLRYLRRKRL 323
 
Name Accession Description Interval E-value
Mrs2_Mfm1p-like cd12823
Saccharomyces cerevisiae inner mitochondrial membrane Mg2+ transporters Mfm1p and Mrs2p-like ...
 55-406 2.08e-107

Saccharomyces cerevisiae inner mitochondrial membrane Mg2+ transporters Mfm1p and Mrs2p-like family; A eukaryotic subfamily belonging to the Escherichia coli CorA-Salmonella typhimurium ZntB_like family (EcCorA_ZntB-like) family of the MIT superfamily of essential membrane proteins involved in transporting divalent cations (uptake or efflux) across membranes. This functionally diverse subfamily includes the inner mitochondrial membrane Mg2+ transporters Saccharomyces cerevisiae Mfm1p/Lpe10p, Mrs2p, and human MRS2/ MRS2L. It also includes a family of Arabidopsis thaliana proteins (AtMGTs) some of which are localized to distinct tissues, and not all of which can transport Mg2+. Structures of the intracellular domain of two EcCorA_ZntB-like family transporters: Vibrio parahaemolyticus and Salmonella typhimurium ZntB form funnel-shaped homopentamers, the tip of the funnel is formed from two C-terminal transmembrane (TM) helices from each monomer, and the large opening of the funnel from the N-terminal cytoplasmic domains. The GMN signature motif of the MIT superfamily occurs just after TM1, mutation within this motif is known to abolish Mg2+ transport through Salmonella typhimurium CorA, and Mrs2p. Natural variants such as GVN and GIN, as in some ZntB family proteins, may be associated with the transport of different divalent cations, such as zinc and cadmium. The functional diversity of MIT transporters may also be due to minor structural differences regulating gating, substrate selection, and transport.


Pssm-ID: 213357  Cd Length: 323  Bit Score: 319.19  E-value: 2.08e-107
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 30688025  55 WTRFDRTGFMEVAGCDKSTIIERSSVSAKDLR----TAFSHSSKILAREKAIVLNLEVIKAVITSEQVMLLDSLR---PE 127
Cdd:cd12823   1 WTVIDSTGNATVVELDKSELLRELGLQPRDLRlldpTLTSYPPSILVRENAILVNLEHIRAIITADEVLLFDPDGsssAL 80

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 30688025 128 VLTLTDRLKHHFPRKDGPEnilqasshghqegGEEGLKSKLPFEFRVLEIAFEVFCSFVDSNVVDLETQAWSILDELTKK 207
Cdd:cd12823  81 VSAFLEELQRRLASSNGSE-------------SESGGEDSLPFEFRALEAALEEVCSHLEAELKRLEPEALPLLDELTDK 147

                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 30688025 208 VSNENLKDLRSLKTSLTHLLARVQKVRDEIEHFLDDKEDMEDLYLTRKWIQNQQTEAAsnsivsqpnlqrhtsnristsm 287
Cdd:cd12823 148 ISTSNLERLLPLKRRLVELETRVQKVRDALEELLDDDEDMADMYLTDKAAGPERLESS---------------------- 205

                       250       260       270       280       290       300       310       320
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 30688025 288 vTEEDDIDDMEMLLEAYFMQLEGMRNKILLMKEHIDSTEAYVKILQNSRRNGLIHLMMLVNIGNYAITAGTVVVNLFGMN 367
Cdd:cd12823 206 -RKEDDHEEVEMLLEAYLQQVDELLNKLEELREYIDDTEELINLILDSRRNQLMRLELKLSIGTLSLAVGTLVAGLFGMN 284

                       330       340       350
                ....*....|....*....|....*....|....*....
gi 30688025 368 IPIGLYSTPDIFGYVVWAVVALCIVLFIVTVGYAKWKKL 406
Cdd:cd12823 285 LPSGLEESPYAFWIVTGGSVVGSILIFIVVLRYLRRKRL 323
CorA pfam01544
CorA-like Mg2+ transporter protein; The CorA transport system is the primary Mg2+ influx ...
 225-403 3.11e-03

CorA-like Mg2+ transporter protein; The CorA transport system is the primary Mg2+ influx system of Salmonella typhimurium and Escherichia coli. CorA is virtually ubiquitous in the Bacteria and Archaea. There are also eukaryotic relatives of this protein. The family includes the MRS2 protein from yeast that is thought to be an RNA splicing protein. However its membership of this family suggests that its effect on splicing is due to altered magnesium levels in the cell.


Pssm-ID: 460246 [Multi-domain]  Cd Length: 292  Bit Score: 39.22  E-value: 3.11e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 30688025   225 HLLARVQKVRDEIEHFLD------DKEDMEDLYLTRKWIQNQQTEAASNSIVSQPNLQRHTSNRISTSMVTEEDDI-DDM 297
Cdd:pfam01544 122 RFLELLEKLEDELDELEDeledetNNELLRELAALRRSLVYLRRSLLPQRDVLNRLLRREDDPLLDDEQKEYLRDLlDRL 201

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 30688025   298 EMLLEAyfmqLEGMRNKILLMKEHIDSteayvkiLQNSRRNgliHLMMLVNIgnyaITA----GTVVVNLFGMNIPiGLY 373
Cdd:pfam01544 202 ERLLED----LDALRERLRSLQDEYSS-------LLSERTN---RVMKVLTV----VTAiflpLTLITGIYGMNFG-GLP 262

                         170       180       190
                  ....*....|....*....|....*....|
gi 30688025   374 STPDIFGYVVWAVVALCIVLFIVTVGYAKW 403
Cdd:pfam01544 263 ELEWSYGYPFVLILGLMILLAILLLFYFRR 292
 
Name Accession Description Interval E-value
Mrs2_Mfm1p-like cd12823
Saccharomyces cerevisiae inner mitochondrial membrane Mg2+ transporters Mfm1p and Mrs2p-like ...
 55-406 2.08e-107

Saccharomyces cerevisiae inner mitochondrial membrane Mg2+ transporters Mfm1p and Mrs2p-like family; A eukaryotic subfamily belonging to the Escherichia coli CorA-Salmonella typhimurium ZntB_like family (EcCorA_ZntB-like) family of the MIT superfamily of essential membrane proteins involved in transporting divalent cations (uptake or efflux) across membranes. This functionally diverse subfamily includes the inner mitochondrial membrane Mg2+ transporters Saccharomyces cerevisiae Mfm1p/Lpe10p, Mrs2p, and human MRS2/ MRS2L. It also includes a family of Arabidopsis thaliana proteins (AtMGTs) some of which are localized to distinct tissues, and not all of which can transport Mg2+. Structures of the intracellular domain of two EcCorA_ZntB-like family transporters: Vibrio parahaemolyticus and Salmonella typhimurium ZntB form funnel-shaped homopentamers, the tip of the funnel is formed from two C-terminal transmembrane (TM) helices from each monomer, and the large opening of the funnel from the N-terminal cytoplasmic domains. The GMN signature motif of the MIT superfamily occurs just after TM1, mutation within this motif is known to abolish Mg2+ transport through Salmonella typhimurium CorA, and Mrs2p. Natural variants such as GVN and GIN, as in some ZntB family proteins, may be associated with the transport of different divalent cations, such as zinc and cadmium. The functional diversity of MIT transporters may also be due to minor structural differences regulating gating, substrate selection, and transport.


Pssm-ID: 213357  Cd Length: 323  Bit Score: 319.19  E-value: 2.08e-107
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 30688025  55 WTRFDRTGFMEVAGCDKSTIIERSSVSAKDLR----TAFSHSSKILAREKAIVLNLEVIKAVITSEQVMLLDSLR---PE 127
Cdd:cd12823   1 WTVIDSTGNATVVELDKSELLRELGLQPRDLRlldpTLTSYPPSILVRENAILVNLEHIRAIITADEVLLFDPDGsssAL 80

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 30688025 128 VLTLTDRLKHHFPRKDGPEnilqasshghqegGEEGLKSKLPFEFRVLEIAFEVFCSFVDSNVVDLETQAWSILDELTKK 207
Cdd:cd12823  81 VSAFLEELQRRLASSNGSE-------------SESGGEDSLPFEFRALEAALEEVCSHLEAELKRLEPEALPLLDELTDK 147

                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 30688025 208 VSNENLKDLRSLKTSLTHLLARVQKVRDEIEHFLDDKEDMEDLYLTRKWIQNQQTEAAsnsivsqpnlqrhtsnristsm 287
Cdd:cd12823 148 ISTSNLERLLPLKRRLVELETRVQKVRDALEELLDDDEDMADMYLTDKAAGPERLESS---------------------- 205

                       250       260       270       280       290       300       310       320
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 30688025 288 vTEEDDIDDMEMLLEAYFMQLEGMRNKILLMKEHIDSTEAYVKILQNSRRNGLIHLMMLVNIGNYAITAGTVVVNLFGMN 367
Cdd:cd12823 206 -RKEDDHEEVEMLLEAYLQQVDELLNKLEELREYIDDTEELINLILDSRRNQLMRLELKLSIGTLSLAVGTLVAGLFGMN 284

                       330       340       350
                ....*....|....*....|....*....|....*....
gi 30688025 368 IPIGLYSTPDIFGYVVWAVVALCIVLFIVTVGYAKWKKL 406
Cdd:cd12823 285 LPSGLEESPYAFWIVTGGSVVGSILIFIVVLRYLRRKRL 323
CorA pfam01544
CorA-like Mg2+ transporter protein; The CorA transport system is the primary Mg2+ influx ...
 225-403 3.11e-03

CorA-like Mg2+ transporter protein; The CorA transport system is the primary Mg2+ influx system of Salmonella typhimurium and Escherichia coli. CorA is virtually ubiquitous in the Bacteria and Archaea. There are also eukaryotic relatives of this protein. The family includes the MRS2 protein from yeast that is thought to be an RNA splicing protein. However its membership of this family suggests that its effect on splicing is due to altered magnesium levels in the cell.


Pssm-ID: 460246 [Multi-domain]  Cd Length: 292  Bit Score: 39.22  E-value: 3.11e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 30688025   225 HLLARVQKVRDEIEHFLD------DKEDMEDLYLTRKWIQNQQTEAASNSIVSQPNLQRHTSNRISTSMVTEEDDI-DDM 297
Cdd:pfam01544 122 RFLELLEKLEDELDELEDeledetNNELLRELAALRRSLVYLRRSLLPQRDVLNRLLRREDDPLLDDEQKEYLRDLlDRL 201

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 30688025   298 EMLLEAyfmqLEGMRNKILLMKEHIDSteayvkiLQNSRRNgliHLMMLVNIgnyaITA----GTVVVNLFGMNIPiGLY 373
Cdd:pfam01544 202 ERLLED----LDALRERLRSLQDEYSS-------LLSERTN---RVMKVLTV----VTAiflpLTLITGIYGMNFG-GLP 262

                         170       180       190
                  ....*....|....*....|....*....|
gi 30688025   374 STPDIFGYVVWAVVALCIVLFIVTVGYAKW 403
Cdd:pfam01544 263 ELEWSYGYPFVLILGLMILLAILLLFYFRR 292
EcCorA_ZntB-like_u2 cd12827
uncharacterized bacterial subfamily of the Escherichia coli CorA-Salmonella typhimurium ZntB ...
 202-407 3.56e-03

uncharacterized bacterial subfamily of the Escherichia coli CorA-Salmonella typhimurium ZntB family; A uncharacterized subfamily of the Escherichia coli CorA-Salmonella typhimurium ZntB (EcCorA-ZntB_like) family of the MIT superfamily of essential membrane proteins involved in transporting divalent cations (uptake or efflux) across membranes.The EcCorA-ZntB-like family includes the Mg2+ transporters Escherichia coli and Salmonella typhimurium CorAs, which can also transport Co2+, and Ni2+. Structures of the intracellular domain of EcCorA-ZntB-like family members, Vibrio parahaemolyticus and Salmonella typhimurium ZntB, form funnel-shaped homopentamers, the tip of the funnel is formed from two C-terminal transmembrane (TM) helices from each monomer, and the large opening of the funnel from the N-terminal cytoplasmic domains. The GMN signature motif of the MIT superfamily occurs just after TM1, mutation within this motif is known to abolish Mg2+ transport through Salmonella typhimurium CorA. Natural variants such as GVN and GIN, such as occur in some ZntB family proteins, may be associated with the transport of different divalent cations, such as zinc and cadmium. The functional diversity of MIT transporters may also be due to minor structural differences regulating gating, substrate selection, and transport.


Pssm-ID: 213361 [Multi-domain]  Cd Length: 289  Bit Score: 39.02  E-value: 3.56e-03
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 30688025 202 DELTKKVSNENLKDLRSLKTSLTHLLARVQKVRDEIEHFL---------DDKEDMEDLYltrkwIQNQQteaasnsivsq 272
Cdd:cd12827 136 QELRKSTKNKELFQLLDLEKSLVYFSTSLKSNEIVLEKLKrsrslkldeEDKELLEDVI-----IENKQ----------- 199

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 30688025 273 pnlqrhtsnriSTSMVTEEDDIddmemlleayfmqLEGMRNkillmkehidsteAYVKILQNsRRNGLIHLMMLVNIGny 352
Cdd:cd12827 200 -----------AIEMAEIYSDI-------------LSGLMD-------------AFASVISN-NLNNVMKFLTSITIV-- 239

                       170       180       190       200       210
                ....*....|....*....|....*....|....*....|....*....|....*
gi 30688025 353 aITAGTVVVNLFGMNIPIGLYSTPDIFgyvvWAVVALCIVLFIVTVGYAKWKKLL 407
Cdd:cd12827 240 -LTIPTIITGFYGMNVPLPFADSPYAF----WIILLISLLLSVIVALILRRKKYF 289
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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