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Conserved domains on  [gi|15224623|ref|NP_180054|]
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Pathogenesis-related thaumatin superfamily protein [Arabidopsis thaliana]

Protein Classification

glycoside hydrolase 64/thaumatin family protein( domain architecture ID 545)

glycoside hydrolase 64 (GH64)/thaumatin family protein may be a beta-1,3-glucanase, a thaumatin, or a thaumatin-like protein (TLP) involved in host defense and a wide range of developmental processes

Graphical summary

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List of domain hits

Name Accession Description Interval E-value
Thaumatin super family cl47527
Thaumatin family;
33-192 3.43e-41

Thaumatin family;


The actual alignment was detected with superfamily member pfam00314:

Pssm-ID: 459757  Cd Length: 211  Bit Score: 138.09  E-value: 3.43e-41
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15224623    33 NRCNFTVWPGILTANRTLLRGGGFALASGSSVNLTVSPE----------------------------------------- 71
Cdd:pfam00314   1 NNCPFTIWPGILTNAGHGPGTGGFELDPGQSRTFTVPDGwsgriwgrtgcsfdgsgscatgdcggglecngaggvppatl 80
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15224623    72 ----------------------NVKM-------HRCKRSTTShtsriqnrllwKEIHRTRFLrresrrrlqrsELRVNGS 122
Cdd:pfam00314  81 aeftlnggggqdfydvslvdgyNLPMsitpsggGGCPVAGCA-----------ADLNSACPA-----------ELQVKSP 138
                         170       180       190       200       210       220       230
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 15224623   123 SG-VVACKSACEAFNKPEYCCTGAFSKPETCPPTKYSKIFKGACPTAYSYVYDYASSRFTCTS-ANYVISFC 192
Cdd:pfam00314 139 GGkVVGCKSACLAFNTDEYCCTGAYATPETCKPTAYSKFFKAACPDAYSYAYDDATSTFTCPGgADYTITFC 210
 
Name Accession Description Interval E-value
Thaumatin pfam00314
Thaumatin family;
33-192 3.43e-41

Thaumatin family;


Pssm-ID: 459757  Cd Length: 211  Bit Score: 138.09  E-value: 3.43e-41
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15224623    33 NRCNFTVWPGILTANRTLLRGGGFALASGSSVNLTVSPE----------------------------------------- 71
Cdd:pfam00314   1 NNCPFTIWPGILTNAGHGPGTGGFELDPGQSRTFTVPDGwsgriwgrtgcsfdgsgscatgdcggglecngaggvppatl 80
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15224623    72 ----------------------NVKM-------HRCKRSTTShtsriqnrllwKEIHRTRFLrresrrrlqrsELRVNGS 122
Cdd:pfam00314  81 aeftlnggggqdfydvslvdgyNLPMsitpsggGGCPVAGCA-----------ADLNSACPA-----------ELQVKSP 138
                         170       180       190       200       210       220       230
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 15224623   123 SG-VVACKSACEAFNKPEYCCTGAFSKPETCPPTKYSKIFKGACPTAYSYVYDYASSRFTCTS-ANYVISFC 192
Cdd:pfam00314 139 GGkVVGCKSACLAFNTDEYCCTGAYATPETCKPTAYSKFFKAACPDAYSYAYDDATSTFTCPGgADYTITFC 210
TLP-PA cd09218
allergenic/antifungal thaumatin-like proteins: plant and animal homologs; This subfamily is ...
28-192 2.61e-39

allergenic/antifungal thaumatin-like proteins: plant and animal homologs; This subfamily is represented by the thaumatin-like proteins (TLPs), Cherry Allergen Pru Av 2 TLP, Peach PpAZ44 TLP (a propylene-induced TLP in abscission), the Caenorhabditis elegans thaumatin family member (thn-6), and other plant and animal homologs. TLPs are involved in host defense and a wide range of developmental processes in fungi, plants, and animals. Due to their inducible expression by environmental stresses such as pathogen/pest attack, drought and cold, plant TLPs are classified as the pathogenesis-related (PR) protein family 5 (PR5). Several members of the plant TLP family have been reported as food allergens from fruits (i.e., cherry, Pru av 2; bell pepper, Cap a1; tomatoes, Lyc e NP24) and pollen allergens from conifers (i.e., mountain cedar, Jun a 3; Arizona cypress, Cup a3; Japanese cedar, Cry j3). TLPs are three-domain, crescent-fold structures with either an electronegative, electropositive, or neutral cleft occurring between domains I and II. It has been proposed that the antifungal activity of plant PR5 proteins relies on the strong electronegative character of this cleft. Some TLPs hydrolyze the beta-1,3-glucans of the type commonly found in fungal walls. TLPs within this subfamily contain 16 conserved Cys residues.


Pssm-ID: 185757  Cd Length: 219  Bit Score: 133.52  E-value: 2.61e-39
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15224623  28 VFTLSNRCNFTVWPGILT-ANRTLLRGGGFALASGSSVNLTVSP---------------ENVKMH----------RCKRS 81
Cdd:cd09218   1 TFTIYNKCPFTVWPGILGnAGHPQLGGGGFELAPGQSRTIDAPSgwsgrfwgrtgcsfdSSGKGScatgdcggglECNGA 80
                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15224623  82 T----------TSHTSRIQN------------RLLWKEIHRTRFLRRESRRR----LQRSELRVNGSSG-VVACKSACEA 134
Cdd:cd09218  81 GgappatlaefTLGGSGGQDfydvslvdgynlPVSITPQGGSGGCRTAGCVAdlnaVCPAELQVKNSGGrVVACKSACLA 160
                       170       180       190       200       210
                ....*....|....*....|....*....|....*....|....*....|....*....
gi 15224623 135 FNKPEYCCTGAFSKPETCPPTKYSKIFKGACPTAYSYVYDYASSRFTCTSA-NYVISFC 192
Cdd:cd09218 161 FNTDEYCCRGAYGTPETCKPTTYSRLFKNACPQAYSYAYDDPTSTFTCSSGaNYVITFC 219
THN smart00205
Thaumatin family; The thaumatin family gathers proteins related to plant pathogenesis. The ...
29-192 8.83e-37

Thaumatin family; The thaumatin family gathers proteins related to plant pathogenesis. The thaumatin family includes very basic members with extracellular and vacuolar localization. Thaumatin itsel is a potent sweet-tasting protein. Several members of this family display significant in vitro activity of inhibiting hyphal growth or spore germination of various fungi probably by a membrane permeabilizing mechanism.


Pssm-ID: 128501 [Multi-domain]  Cd Length: 218  Bit Score: 127.19  E-value: 8.83e-37
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15224623     29 FTLSNRCNFTVWPGILTANRTLLRGGGFALASGSSVNLTVsPENVKMHR----------------CKR-------STTSH 85
Cdd:smart00205   1 FEFVNNCPYTVWAAALPSGKPQLSGGGFELNSGASWQLDA-PPGTKMGRiwartgcnfdasgrgrCATgdcggvlQCNGW 79
                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15224623     86 TSRIQNRLLWKEIHR------------------------------TRFLRRESRRRLQRSELRVNGSSGVVACKSACEAF 135
Cdd:smart00205  80 GGRPPATLAEFALNQfggldfydvslvdgfnipmsftptggsgdcKGAGCTADLNAQCPAELQVPGGGSVVACNSACTVF 159
                          170       180       190       200       210
                   ....*....|....*....|....*....|....*....|....*....|....*...
gi 15224623    136 NKPEYCCTGAFSKPETCPPTKYSKIFKGACPTAYSYVYDYASSRFTCTS-ANYVISFC 192
Cdd:smart00205 160 GTDQYCCTGGQNNPETCPPTNYSRIFKNACPDAYSYAYDDPTSTFTCTGgTNYKVTFC 217
 
Name Accession Description Interval E-value
Thaumatin pfam00314
Thaumatin family;
33-192 3.43e-41

Thaumatin family;


Pssm-ID: 459757  Cd Length: 211  Bit Score: 138.09  E-value: 3.43e-41
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15224623    33 NRCNFTVWPGILTANRTLLRGGGFALASGSSVNLTVSPE----------------------------------------- 71
Cdd:pfam00314   1 NNCPFTIWPGILTNAGHGPGTGGFELDPGQSRTFTVPDGwsgriwgrtgcsfdgsgscatgdcggglecngaggvppatl 80
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15224623    72 ----------------------NVKM-------HRCKRSTTShtsriqnrllwKEIHRTRFLrresrrrlqrsELRVNGS 122
Cdd:pfam00314  81 aeftlnggggqdfydvslvdgyNLPMsitpsggGGCPVAGCA-----------ADLNSACPA-----------ELQVKSP 138
                         170       180       190       200       210       220       230
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 15224623   123 SG-VVACKSACEAFNKPEYCCTGAFSKPETCPPTKYSKIFKGACPTAYSYVYDYASSRFTCTS-ANYVISFC 192
Cdd:pfam00314 139 GGkVVGCKSACLAFNTDEYCCTGAYATPETCKPTAYSKFFKAACPDAYSYAYDDATSTFTCPGgADYTITFC 210
TLP-PA cd09218
allergenic/antifungal thaumatin-like proteins: plant and animal homologs; This subfamily is ...
28-192 2.61e-39

allergenic/antifungal thaumatin-like proteins: plant and animal homologs; This subfamily is represented by the thaumatin-like proteins (TLPs), Cherry Allergen Pru Av 2 TLP, Peach PpAZ44 TLP (a propylene-induced TLP in abscission), the Caenorhabditis elegans thaumatin family member (thn-6), and other plant and animal homologs. TLPs are involved in host defense and a wide range of developmental processes in fungi, plants, and animals. Due to their inducible expression by environmental stresses such as pathogen/pest attack, drought and cold, plant TLPs are classified as the pathogenesis-related (PR) protein family 5 (PR5). Several members of the plant TLP family have been reported as food allergens from fruits (i.e., cherry, Pru av 2; bell pepper, Cap a1; tomatoes, Lyc e NP24) and pollen allergens from conifers (i.e., mountain cedar, Jun a 3; Arizona cypress, Cup a3; Japanese cedar, Cry j3). TLPs are three-domain, crescent-fold structures with either an electronegative, electropositive, or neutral cleft occurring between domains I and II. It has been proposed that the antifungal activity of plant PR5 proteins relies on the strong electronegative character of this cleft. Some TLPs hydrolyze the beta-1,3-glucans of the type commonly found in fungal walls. TLPs within this subfamily contain 16 conserved Cys residues.


Pssm-ID: 185757  Cd Length: 219  Bit Score: 133.52  E-value: 2.61e-39
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15224623  28 VFTLSNRCNFTVWPGILT-ANRTLLRGGGFALASGSSVNLTVSP---------------ENVKMH----------RCKRS 81
Cdd:cd09218   1 TFTIYNKCPFTVWPGILGnAGHPQLGGGGFELAPGQSRTIDAPSgwsgrfwgrtgcsfdSSGKGScatgdcggglECNGA 80
                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15224623  82 T----------TSHTSRIQN------------RLLWKEIHRTRFLRRESRRR----LQRSELRVNGSSG-VVACKSACEA 134
Cdd:cd09218  81 GgappatlaefTLGGSGGQDfydvslvdgynlPVSITPQGGSGGCRTAGCVAdlnaVCPAELQVKNSGGrVVACKSACLA 160
                       170       180       190       200       210
                ....*....|....*....|....*....|....*....|....*....|....*....
gi 15224623 135 FNKPEYCCTGAFSKPETCPPTKYSKIFKGACPTAYSYVYDYASSRFTCTSA-NYVISFC 192
Cdd:cd09218 161 FNTDEYCCRGAYGTPETCKPTTYSRLFKNACPQAYSYAYDDPTSTFTCSSGaNYVITFC 219
THN smart00205
Thaumatin family; The thaumatin family gathers proteins related to plant pathogenesis. The ...
29-192 8.83e-37

Thaumatin family; The thaumatin family gathers proteins related to plant pathogenesis. The thaumatin family includes very basic members with extracellular and vacuolar localization. Thaumatin itsel is a potent sweet-tasting protein. Several members of this family display significant in vitro activity of inhibiting hyphal growth or spore germination of various fungi probably by a membrane permeabilizing mechanism.


Pssm-ID: 128501 [Multi-domain]  Cd Length: 218  Bit Score: 127.19  E-value: 8.83e-37
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15224623     29 FTLSNRCNFTVWPGILTANRTLLRGGGFALASGSSVNLTVsPENVKMHR----------------CKR-------STTSH 85
Cdd:smart00205   1 FEFVNNCPYTVWAAALPSGKPQLSGGGFELNSGASWQLDA-PPGTKMGRiwartgcnfdasgrgrCATgdcggvlQCNGW 79
                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15224623     86 TSRIQNRLLWKEIHR------------------------------TRFLRRESRRRLQRSELRVNGSSGVVACKSACEAF 135
Cdd:smart00205  80 GGRPPATLAEFALNQfggldfydvslvdgfnipmsftptggsgdcKGAGCTADLNAQCPAELQVPGGGSVVACNSACTVF 159
                          170       180       190       200       210
                   ....*....|....*....|....*....|....*....|....*....|....*...
gi 15224623    136 NKPEYCCTGAFSKPETCPPTKYSKIFKGACPTAYSYVYDYASSRFTCTS-ANYVISFC 192
Cdd:smart00205 160 GTDQYCCTGGQNNPETCPPTNYSRIFKNACPDAYSYAYDDPTSTFTCTGgTNYKVTFC 217
Thaumatin-like cd09215
the sweet-tasting protein, thaumatin, and thaumatin-like proteins involved in host defense; ...
29-192 3.78e-13

the sweet-tasting protein, thaumatin, and thaumatin-like proteins involved in host defense; This family is represented by the sweet-tasting protein thaumatin from the African berry Thaumatococcus daniellii and thaumatin-like proteins (TLPs) involved in host defense and a wide range of developmental processes in fungi, plants, and animals. Plant TLPs are classified as pathogenesis-related (PR) protein family 5 (PR5), their expression is induced by environmental stresses such as pathogen/pest attack, drought and cold. TLPs included in this family are such proteins as zeamatin, found in high concentrations in cereal seeds; osmotin, a salt-induced protein in osmotically stressed plants; and PpAZ44, a propylene-induced TLP in abscission of young fruit. Several members of the plant TLP family have been reported as food allergens from fruits (i.e., cherry, Pru av 2; bell pepper, Cap a1; tomatoes, Lyc e NP24) and pollen allergens from conifers (i.e., mountain cedar, Jun a 3; Arizona cypress, Cup a3; Japanese cedar, Cry j3). Thaumatin and TLPs are three-domain, crescent-fold structures with either an electronegative, electropositive, or neutral cleft occurring between domains I and II. It has been proposed that the antifungal activity of plant PR5 proteins relies on the strong electronegative character of this cleft. Some TLPs hydrolyze the beta-1,3-glucans of the type commonly found in fungal walls. Most TLPs contain 16 conserved Cys residues. A deletion within the third domain (domain II) of the Triticum aestivum thaumatin-like xylanase inhibitor is observed, thus, only 10 conserved Cys residues are present within this smaller TLP and similar homologs.


Pssm-ID: 185754  Cd Length: 157  Bit Score: 64.01  E-value: 3.78e-13
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15224623  29 FTLSNRCNFTVWPGILTANRTLLRGGGFALASGSSVNLTVSPENVKMHRCKRSTTSHTSRIQNRLLWKEIHRTRFLRRES 108
Cdd:cd09215   1 FTITNRCPYTIWPAIFTQVGKGPYTGGFELNPGETKSFDVSAGWQGRIWARTNCSFNANSGGNACLTGDCNGGLNCQGTG 80
                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15224623 109 RRRLQRSELRVNGSSGVVACK-SACEAFNKP-EYCCTGAFSKPETCPptkyskifkgACPTAYSYVYDYASSRFTCTS-A 185
Cdd:cd09215  81 GPPATLAEFTLSGGGGLDYYDiSLVDGYNLPmSITPQPGECPTPICA----------ACPDAYSYAYDDQTSTFTCPGgA 150

                ....*..
gi 15224623 186 NYVISFC 192
Cdd:cd09215 151 GYEVVFC 157
TLP-F cd09219
thaumatin-like proteins: basidiomycete homologs; This subfamily is represented by Lentinula ...
120-192 4.95e-12

thaumatin-like proteins: basidiomycete homologs; This subfamily is represented by Lentinula edodes TLG1, a thaumatin-like protein (TLP), as well as, other basidiomycete homologs. In general, TLPs are involved in host defense and a wide range of developmental processes in fungi, plants, and animals. TLG1 TLP is involved in lentinan degradation and fruiting body senescence. TLG1 expressed in Escherichia coli and Aspergillus oryzae exhibited beta-1,3-glucanase activity and demonstrated lentinan degrading activity. TLG1 is proposed to be involved in lentinan and cell wall degradation during senescence following harvest and spore diffusion. TLPs are three-domain, crescent-fold structures with either an electronegative, electropositive, or neutral cleft occurring between domains I and II. TLG1 from Lentinula edodes contains the required acidic amino acids conserved in the appropriate positions to possess an electronegative cleft. TLPs within this subfamily contain 13 conserved Cys residues; the number of total Cys residues in these TLPs varies from 16 in L. edodes TLG1 to 18 in other basidiomycete homologs.


Pssm-ID: 185758  Cd Length: 229  Bit Score: 62.33  E-value: 4.95e-12
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15224623 120 NGSSGVVACKSACEA---FNKPEYCCTGAFSKPETCPPT--KYSKIFKGACPTAYSYVYDYASSR--FTC---TSANYVI 189
Cdd:cd09219 146 DQKGVNLGCISPCNRdknHDDSPSCCTGSHNKPETCPQSgvGNYAYFKDNCPTAYAYAYDEKSHTalWTCgdsKSADYTV 225

                ...
gi 15224623 190 SFC 192
Cdd:cd09219 226 TFC 228
TLP-P cd09217
thaumatin and allergenic/antifungal thaumatin-like proteins: plant homologs; This subfamily is ...
29-192 3.01e-04

thaumatin and allergenic/antifungal thaumatin-like proteins: plant homologs; This subfamily is represented by the sweet-tasting protein thaumatin from the African berry Thaumatococcus daniellii, allergenic/antifungal Thaumatin-like proteins (TLPs), and related plant proteins. TLPs are involved in host defense and a wide range of developmental processes in fungi, plants, and animals. Plant TLPs are classified as pathogenesis-related (PR) protein family 5 (PR5), their expression is induced by environmental stresses such as pathogen/pest attack, drought and cold. TLPs in this subfamily include such proteins as zeamatin, found in high concentrations in cereal seeds, and osmotin, a salt-induced protein in osmotically stressed plants. Several members of the plant TLP family have been reported as food allergens from fruits (i.e., cherry, Pru av 2; bell pepper, Cap a1; tomatoes, Lyc e NP24) and pollen allergens from conifers (i.e., mountain cedar, Jun a 3; Arizona cypress, Cup a3; Japanese cedar, Cry j3). Thaumatin and TLPs are three-domain, crescent-fold structures with either an electronegative, electropositive, or neutral cleft occurring between domains I and II. It has been proposed that the antifungal activity of plant PR5 proteins relies on the strong electronegative character of this cleft. IgE-binding epitopes of mountain Cedar (Juniperus ashei) allergen Jun a 3, which interact with pooled IgE from patients suffering allergenic response to this allergen, were mainly located on the helical domain II; the best-conserved IgE-binding epitope predicted for TLPs corresponds to this region. Some TLPs hydrolyze the beta-1,3-glucans of the type commonly found in fungal walls. Most TLPs contain 16 conserved Cys residues. A deletion within the third domain (domain II) of the Triticum aestivum thaumatin-like xylanase inhibitor is observed, thus, only 10 conserved Cys residues are present within this smaller TLP and similar homologs.


Pssm-ID: 185756  Cd Length: 151  Bit Score: 39.29  E-value: 3.01e-04
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15224623  29 FTLSNRCNFTVWPGiltanrTLLRGGGFALASGSSVNLTVsPENVKMHRCkRSTTSHTSRIQNRLLWK--EIHRTRFLRR 106
Cdd:cd09217   1 FTITNNCGYTVWPA------ATPVGGGRQLNPGQSWTIDV-PAGTAGGRI-WGRTGCSFDASGRGSCQtgDCGGVLSCTG 72
                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15224623 107 ESRRRLQRSELRVNGSSGVVACKSACEAFNKP-EYCCTGAFSKPETCpptkyskifKGACPTAYSYVYDyASSRFTCTS- 184
Cdd:cd09217  73 SGKPPATLAEYTLNQSGQDFYDISLVDGFNVPmDFSPTGGGCHAIPC---------AANCPDAYSYPKD-PTTTFTCPGg 142

                ....*...
gi 15224623 185 ANYVISFC 192
Cdd:cd09217 143 TNYRIVFC 150
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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