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Conserved domains on  [gi|334184414|ref|NP_180027|]
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F-box associated ubiquitination effector family protein [Arabidopsis thaliana]

Protein Classification

F-box protein( domain architecture ID 18135504)

F-box protein functions as the substrate-recognition component of an SCF (Skp1-Cullin-F-box protein) ubiquitin-protein ligase that is involved in ubiquitin-dependent degradation

CATH:  1.20.1280.50
Gene Ontology:  GO:0006511|GO:0019005|GO:0005515
PubMed:  31898225|11178263
SCOP:  4001927

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
FBA_1 pfam07734
F-box associated; Most of these proteins contain pfam00646 at the N terminus, suggesting that ...
 120-248 3.56e-44

F-box associated; Most of these proteins contain pfam00646 at the N terminus, suggesting that they are effectors linked with ubiquitination.


:

Pssm-ID: 254394  Cd Length: 159  Bit Score: 146.60  E-value: 3.56e-44
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 334184414  120 LKRNTYWVAGDKE--MGMFMLYFDFTRERFGHF-PLPFKSfNREDTASLSVVREEKLSVLHQkiLEFSNEMKIWLTNKID 196
Cdd:pfam07734   4 LKGNTYWLAREKEtnEEDFLLSFDFTTERFGPRlPLPFQS-PDLDTVSLSVVREEKLAVLLQ--EDDTSKIEIWVTTKIE 80

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|...
gi 334184414  197 ETiELSWSNFvLTVDYDKFN-LPSVVNVASFLLDEENKVAVCSDIDVDDENRT 248
Cdd:pfam07734  81 PN-AVSWSKF-LTVDMPPLPgLFFHFFAGSFFIDEEKKVAVVCDKDKEKKTNT 131
F-box_SF super family cl45894
F-box domain superfamily; This short domain is commonly found at the N-terminus of various ...
 3-27 6.70e-04

F-box domain superfamily; This short domain is commonly found at the N-terminus of various proteins, and typically co-occurs with one or more other conserved domains or motifs, such as leucine rich repeats, WD40 repeats, kelch, tub, spry, and others. The F-box domain has a role in mediating protein-protein interactions in a variety of contexts, such as polyubiquitination, transcription elongation, centromere binding and translational repression. One of the best researched roles of F-box proteins is their participation in SCF (Skp1-Cul1-F-box protein), a multi-protein complex that functions as a ubiquitin E3 ligase, where the role of the F-box protein is to recruit target substrates. Gene families containing the F-box are found greatly expanded in narrow taxonomic lineages, such as flowering plants and nematodes. In this hierarchical classification, many of the subfamilies are named according to their domain architectures.


The actual alignment was detected with superfamily member cd22157:

Pssm-ID: 459239  Cd Length: 39  Bit Score: 36.29  E-value: 6.70e-04
                         10        20
                 ....*....|....*....|....*
gi 334184414   3 SDLPDDLESEILSRVPTKSLAKLQT 27
Cdd:cd22157    1 SSLPDDLVEEILSRLPAKSLLRFRC 25
 
Name Accession Description Interval E-value
FBA_1 pfam07734
F-box associated; Most of these proteins contain pfam00646 at the N terminus, suggesting that ...
 120-248 3.56e-44

F-box associated; Most of these proteins contain pfam00646 at the N terminus, suggesting that they are effectors linked with ubiquitination.


Pssm-ID: 254394  Cd Length: 159  Bit Score: 146.60  E-value: 3.56e-44
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 334184414  120 LKRNTYWVAGDKE--MGMFMLYFDFTRERFGHF-PLPFKSfNREDTASLSVVREEKLSVLHQkiLEFSNEMKIWLTNKID 196
Cdd:pfam07734   4 LKGNTYWLAREKEtnEEDFLLSFDFTTERFGPRlPLPFQS-PDLDTVSLSVVREEKLAVLLQ--EDDTSKIEIWVTTKIE 80

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|...
gi 334184414  197 ETiELSWSNFvLTVDYDKFN-LPSVVNVASFLLDEENKVAVCSDIDVDDENRT 248
Cdd:pfam07734  81 PN-AVSWSKF-LTVDMPPLPgLFFHFFAGSFFIDEEKKVAVVCDKDKEKKTNT 131
F_box_assoc_1 TIGR01640
F-box protein interaction domain; This model describes a large family of plant domains, with ...
 27-241 6.03e-32

F-box protein interaction domain; This model describes a large family of plant domains, with several hundred members in Arabidopsis thaliana. Most examples are found C-terminal to an F-box (pfam00646), a 60 amino acid motif involved in ubiquitination of target proteins to mark them for degradation. Two-hybid experiments support the idea that most members are interchangeable F-box subunits of SCF E3 complexes. Some members have two copies of this domain.


Pssm-ID: 273726 [Multi-domain]  Cd Length: 230  Bit Score: 117.46  E-value: 6.03e-32
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 334184414   27 TMGNTRLVVWDSSTGETRWI---KPRTRFRNDDYYGLGYVNSKSSghsFKILRSCY-YRNDQKQwvaEFEIYEFGSDTWR 102
Cdd:TIGR01640  10 FSYGKRLVVWNPSTGQSRWLptpKSRRSNKESDTYFLGYDPIEKQ---YKVLCFSDrSGNRNQS---EHQVYTLGSNSWR 83

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 334184414  103 VLDYFTHDYGVFSSGGPLKRNTYWVAGDKEM--GMFMLYFDFTRERFGHF-PLPFKSFNREDTASLSVVReEKLSVLHQK 179
Cdd:TIGR01640  84 TIECSPPHHPLKSRGVCINGVLYYLAYTLKTnpDYFIVSFDVSSERFKEFiPLPCGNSDSVDYLSLINYK-GKLAVLKQK 162

                         170       180       190       200       210       220
                  ....*....|....*....|....*....|....*....|....*....|....*....|...
gi 334184414  180 ilEFSNEMKIWLTNkidETIELSWS-NFVLTVDYDKfNLPSVVNVaSFLLDEENKVAVCSDID 241
Cdd:TIGR01640 163 --KDTNNFDLWVLN---DAGKQEWSkLFTVPIPPLP-DLVDDNFL-SGFTDKGEIVLCCEDEN 218
F-box_AtFBW1-like cd22157
F-box domain found in Arabidopsis thaliana F-box/WD-40 repeat-containing protein 1 (AtFBW1) ...
 3-27 6.70e-04

F-box domain found in Arabidopsis thaliana F-box/WD-40 repeat-containing protein 1 (AtFBW1) and similar proteins; AtFBW1, also called WD-40-associated F-box protein 1, is an F-box protein that contains four WD-40 repeats, which are separated from each other by a spacer region. Like other F-box proteins, AtFBW1 may be a component of SCF (Skp1 Cdc53 F-box protein) E3 ubiquitin ligase complexes, which may mediate the ubiquitination and subsequent proteasomal degradation of target proteins. This subfamily also contains many F-box only proteins that do not have any WD repeat. The F-box domain has a role in mediating protein-protein interactions in a variety of contexts, such as polyubiquitination, transcription elongation, centromere binding and translational repression.


Pssm-ID: 438928  Cd Length: 39  Bit Score: 36.29  E-value: 6.70e-04
                         10        20
                 ....*....|....*....|....*
gi 334184414   3 SDLPDDLESEILSRVPTKSLAKLQT 27
Cdd:cd22157    1 SSLPDDLVEEILSRLPAKSLLRFRC 25
 
Name Accession Description Interval E-value
FBA_1 pfam07734
F-box associated; Most of these proteins contain pfam00646 at the N terminus, suggesting that ...
 120-248 3.56e-44

F-box associated; Most of these proteins contain pfam00646 at the N terminus, suggesting that they are effectors linked with ubiquitination.


Pssm-ID: 254394  Cd Length: 159  Bit Score: 146.60  E-value: 3.56e-44
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 334184414  120 LKRNTYWVAGDKE--MGMFMLYFDFTRERFGHF-PLPFKSfNREDTASLSVVREEKLSVLHQkiLEFSNEMKIWLTNKID 196
Cdd:pfam07734   4 LKGNTYWLAREKEtnEEDFLLSFDFTTERFGPRlPLPFQS-PDLDTVSLSVVREEKLAVLLQ--EDDTSKIEIWVTTKIE 80

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|...
gi 334184414  197 ETiELSWSNFvLTVDYDKFN-LPSVVNVASFLLDEENKVAVCSDIDVDDENRT 248
Cdd:pfam07734  81 PN-AVSWSKF-LTVDMPPLPgLFFHFFAGSFFIDEEKKVAVVCDKDKEKKTNT 131
F_box_assoc_1 TIGR01640
F-box protein interaction domain; This model describes a large family of plant domains, with ...
 27-241 6.03e-32

F-box protein interaction domain; This model describes a large family of plant domains, with several hundred members in Arabidopsis thaliana. Most examples are found C-terminal to an F-box (pfam00646), a 60 amino acid motif involved in ubiquitination of target proteins to mark them for degradation. Two-hybid experiments support the idea that most members are interchangeable F-box subunits of SCF E3 complexes. Some members have two copies of this domain.


Pssm-ID: 273726 [Multi-domain]  Cd Length: 230  Bit Score: 117.46  E-value: 6.03e-32
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 334184414   27 TMGNTRLVVWDSSTGETRWI---KPRTRFRNDDYYGLGYVNSKSSghsFKILRSCY-YRNDQKQwvaEFEIYEFGSDTWR 102
Cdd:TIGR01640  10 FSYGKRLVVWNPSTGQSRWLptpKSRRSNKESDTYFLGYDPIEKQ---YKVLCFSDrSGNRNQS---EHQVYTLGSNSWR 83

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 334184414  103 VLDYFTHDYGVFSSGGPLKRNTYWVAGDKEM--GMFMLYFDFTRERFGHF-PLPFKSFNREDTASLSVVReEKLSVLHQK 179
Cdd:TIGR01640  84 TIECSPPHHPLKSRGVCINGVLYYLAYTLKTnpDYFIVSFDVSSERFKEFiPLPCGNSDSVDYLSLINYK-GKLAVLKQK 162

                         170       180       190       200       210       220
                  ....*....|....*....|....*....|....*....|....*....|....*....|...
gi 334184414  180 ilEFSNEMKIWLTNkidETIELSWS-NFVLTVDYDKfNLPSVVNVaSFLLDEENKVAVCSDID 241
Cdd:TIGR01640 163 --KDTNNFDLWVLN---DAGKQEWSkLFTVPIPPLP-DLVDDNFL-SGFTDKGEIVLCCEDEN 218
F-box_AtFBW1-like cd22157
F-box domain found in Arabidopsis thaliana F-box/WD-40 repeat-containing protein 1 (AtFBW1) ...
 3-27 6.70e-04

F-box domain found in Arabidopsis thaliana F-box/WD-40 repeat-containing protein 1 (AtFBW1) and similar proteins; AtFBW1, also called WD-40-associated F-box protein 1, is an F-box protein that contains four WD-40 repeats, which are separated from each other by a spacer region. Like other F-box proteins, AtFBW1 may be a component of SCF (Skp1 Cdc53 F-box protein) E3 ubiquitin ligase complexes, which may mediate the ubiquitination and subsequent proteasomal degradation of target proteins. This subfamily also contains many F-box only proteins that do not have any WD repeat. The F-box domain has a role in mediating protein-protein interactions in a variety of contexts, such as polyubiquitination, transcription elongation, centromere binding and translational repression.


Pssm-ID: 438928  Cd Length: 39  Bit Score: 36.29  E-value: 6.70e-04
                         10        20
                 ....*....|....*....|....*
gi 334184414   3 SDLPDDLESEILSRVPTKSLAKLQT 27
Cdd:cd22157    1 SSLPDDLVEEILSRLPAKSLLRFRC 25
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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