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Conserved domains on  [gi|15227287|ref|NP_179262|]
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Iron-sulfur cluster biosynthesis family protein [Arabidopsis thaliana]

Protein Classification

HesB/IscA family protein( domain architecture ID 10001059)

HesB/IscA family protein is a scaffold protein upon which 2Fe-2S clusters are assembled and subsequently transferred to acceptor proteins; similar to iron-sulfur assembly protein IscA that is involved in the maturation of mitochondrial 4Fe-4S proteins functioning late in the iron-sulfur cluster assembly pathway

Gene Ontology:  GO:0016226|GO:0051536
PubMed:  32108236

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
IscA COG0316
Fe-S cluster assembly iron-binding protein IscA [Posttranslational modification, protein ...
 22-124 8.11e-47

Fe-S cluster assembly iron-binding protein IscA [Posttranslational modification, protein turnover, chaperones];


:

Pssm-ID: 440085 [Multi-domain]  Cd Length: 107  Bit Score: 146.84  E-value: 8.11e-47
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15227287  22 VLTLTDEAASRVHHLLQQRQKP--FLRLGVKARGCNGLSYTLNYADEKGKFDELVEEKGVRILVEPKALMHVIGTKMDFV 99
Cdd:COG0316   1 PITLTDAAAKRIKRLLAKEGNPglGLRVGVKGGGCSGFSYGLDFDDEPNEDDLVFEQDGVKVVVDPKSLPYLDGTEIDYV 80

                        90       100
                ....*....|....*....|....*
gi 15227287 100 DDKLRSEFVFINPNSQGQCGCGESF 124
Cdd:COG0316  81 EELLGSGFKFNNPNAKSSCGCGESF 105
 
Name Accession Description Interval E-value
IscA COG0316
Fe-S cluster assembly iron-binding protein IscA [Posttranslational modification, protein ...
 22-124 8.11e-47

Fe-S cluster assembly iron-binding protein IscA [Posttranslational modification, protein turnover, chaperones];


Pssm-ID: 440085 [Multi-domain]  Cd Length: 107  Bit Score: 146.84  E-value: 8.11e-47
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15227287  22 VLTLTDEAASRVHHLLQQRQKP--FLRLGVKARGCNGLSYTLNYADEKGKFDELVEEKGVRILVEPKALMHVIGTKMDFV 99
Cdd:COG0316   1 PITLTDAAAKRIKRLLAKEGNPglGLRVGVKGGGCSGFSYGLDFDDEPNEDDLVFEQDGVKVVVDPKSLPYLDGTEIDYV 80

                        90       100
                ....*....|....*....|....*
gi 15227287 100 DDKLRSEFVFINPNSQGQCGCGESF 124
Cdd:COG0316  81 EELLGSGFKFNNPNAKSSCGCGESF 105
TIGR00049 TIGR00049
Iron-sulfur cluster assembly accessory protein; Proteins in this subfamily appear to be ...
 24-126 8.70e-46

Iron-sulfur cluster assembly accessory protein; Proteins in this subfamily appear to be associated with the process of FeS-cluster assembly. The HesB proteins are associated with the nif gene cluster and the Rhizobium gene IscN has been shown to be required for nitrogen fixation. Nitrogenase includes multiple FeS clusters and many genes for their assembly. The E. coli SufA protein is associated with SufS, a NifS homolog and SufD which are involved in the FeS cluster assembly of the FhnF protein. The Azotobacter protein IscA (homologs of which are also found in E.coli) is associated which IscS, another NifS homolog and IscU, a nifU homolog as well as other factors consistent with a role in FeS cluster chemistry. A homolog from Geobacter contains a selenocysteine in place of an otherwise invariant cysteine, further suggesting a role in redox chemistry. [Biosynthesis of cofactors, prosthetic groups, and carriers, Other]


Pssm-ID: 272875 [Multi-domain]  Cd Length: 105  Bit Score: 144.26  E-value: 8.70e-46
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15227287    24 TLTDEAASRVHHLLQQRQKP--FLRLGVKARGCNGLSYTLNYADEKGKFDELVEEKGVRILVEPKALMHVIGTKMDFVDD 101
Cdd:TIGR00049   1 TLTDSAAKRIKALLAGEGEPnlGLRVGVKGGGCSGLQYGLEFDDEPNEDDEVFEQDGVKVVVDPKSLPYLDGSEIDYVEE 80

                          90       100
                  ....*....|....*....|....*
gi 15227287   102 KLRSEFVFINPNSQGQCGCGESFMT 126
Cdd:TIGR00049  81 LLGSGFTFTNPNAKGTCGCGKSFSV 105
iscA PRK09502
iron-sulfur cluster assembly protein IscA;
23-124 1.78e-30

iron-sulfur cluster assembly protein IscA;


Pssm-ID: 181914 [Multi-domain]  Cd Length: 107  Bit Score: 105.34  E-value: 1.78e-30
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15227287   23 LTLTDEAASRVHHLLQQRQKPF-LRLGVKARGCNGLSYTLNYADEKGKFDELVEEKGVRILVEPKALMHVIGTKMDFVDD 101
Cdd:PRK09502   3 ITLSDSAAARVNTFLANRGKGFgLRLGVRTSGCSGMAYVLEFVDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKE 82

                         90       100
                 ....*....|....*....|...
gi 15227287  102 KLRSEFVFINPNSQGQCGCGESF 124
Cdd:PRK09502  83 GLNEGFKFTNPNVKDECGCGESF 105
Fe-S_biosyn pfam01521
Iron-sulphur cluster biosynthesis; This family is involved in iron-sulphur cluster ...
 23-122 1.84e-14

Iron-sulphur cluster biosynthesis; This family is involved in iron-sulphur cluster biosynthesis. Its members include proteins that are involved in nitrogen fixation such as the HesB and HesB-like proteins.


Pssm-ID: 426304  Cd Length: 111  Bit Score: 64.59  E-value: 1.84e-14
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15227287    23 LTLTDEAASRVHHLLQQRQKPfLRLGVK------ARGC--NGLSYTLNYADEK-GKFDELVEEKGVRILVEPKALMHV-I 92
Cdd:pfam01521   3 ITITDAAAERLKKLLAGDKKE-LRLDVDdgggpySKGGcsIGGKFSLVLVDEPdPDYDEVIESNGGPIYVDSYSLPFLdE 81

                          90       100       110
                  ....*....|....*....|....*....|
gi 15227287    93 GTKMDFVDDKLRSEFVFINPNSQGQCGCGE 122
Cdd:pfam01521  82 GLTLDFVEDLGTLGLKSDNGNLDGNVGCGD 111
 
Name Accession Description Interval E-value
IscA COG0316
Fe-S cluster assembly iron-binding protein IscA [Posttranslational modification, protein ...
 22-124 8.11e-47

Fe-S cluster assembly iron-binding protein IscA [Posttranslational modification, protein turnover, chaperones];


Pssm-ID: 440085 [Multi-domain]  Cd Length: 107  Bit Score: 146.84  E-value: 8.11e-47
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15227287  22 VLTLTDEAASRVHHLLQQRQKP--FLRLGVKARGCNGLSYTLNYADEKGKFDELVEEKGVRILVEPKALMHVIGTKMDFV 99
Cdd:COG0316   1 PITLTDAAAKRIKRLLAKEGNPglGLRVGVKGGGCSGFSYGLDFDDEPNEDDLVFEQDGVKVVVDPKSLPYLDGTEIDYV 80

                        90       100
                ....*....|....*....|....*
gi 15227287 100 DDKLRSEFVFINPNSQGQCGCGESF 124
Cdd:COG0316  81 EELLGSGFKFNNPNAKSSCGCGESF 105
TIGR00049 TIGR00049
Iron-sulfur cluster assembly accessory protein; Proteins in this subfamily appear to be ...
 24-126 8.70e-46

Iron-sulfur cluster assembly accessory protein; Proteins in this subfamily appear to be associated with the process of FeS-cluster assembly. The HesB proteins are associated with the nif gene cluster and the Rhizobium gene IscN has been shown to be required for nitrogen fixation. Nitrogenase includes multiple FeS clusters and many genes for their assembly. The E. coli SufA protein is associated with SufS, a NifS homolog and SufD which are involved in the FeS cluster assembly of the FhnF protein. The Azotobacter protein IscA (homologs of which are also found in E.coli) is associated which IscS, another NifS homolog and IscU, a nifU homolog as well as other factors consistent with a role in FeS cluster chemistry. A homolog from Geobacter contains a selenocysteine in place of an otherwise invariant cysteine, further suggesting a role in redox chemistry. [Biosynthesis of cofactors, prosthetic groups, and carriers, Other]


Pssm-ID: 272875 [Multi-domain]  Cd Length: 105  Bit Score: 144.26  E-value: 8.70e-46
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15227287    24 TLTDEAASRVHHLLQQRQKP--FLRLGVKARGCNGLSYTLNYADEKGKFDELVEEKGVRILVEPKALMHVIGTKMDFVDD 101
Cdd:TIGR00049   1 TLTDSAAKRIKALLAGEGEPnlGLRVGVKGGGCSGLQYGLEFDDEPNEDDEVFEQDGVKVVVDPKSLPYLDGSEIDYVEE 80

                          90       100
                  ....*....|....*....|....*
gi 15227287   102 KLRSEFVFINPNSQGQCGCGESFMT 126
Cdd:TIGR00049  81 LLGSGFTFTNPNAKGTCGCGKSFSV 105
sufA_proteo TIGR01997
FeS assembly scaffold SufA; This model represents the SufA protein of the SUF system of ...
 21-124 9.00e-34

FeS assembly scaffold SufA; This model represents the SufA protein of the SUF system of iron-sulfur cluster biosynthesis. This system performs FeS biosynthesis even during oxidative stress and tends to be absent in obligate anaerobic and microaerophilic bacteria. [Biosynthesis of cofactors, prosthetic groups, and carriers, Other]


Pssm-ID: 131052  Cd Length: 107  Bit Score: 113.77  E-value: 9.00e-34
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15227287    21 QVLTLTDEAASRVHHLLQQR-QKPFLRLGVKARGCNGLSYTLNYADEKGKFDELVEEKGVRILVEPKALMHVIGTKMDFV 99
Cdd:TIGR01997   1 AVITLTDAAAIHIRELVAKRpEAVGIRLGVKKTGCAGMEYVLDLVSEPKKDDDLIEHDGAKVFVAPEAVLFILGTQVDFV 80

                          90       100
                  ....*....|....*....|....*
gi 15227287   100 DDKLRSEFVFINPNSQGQCGCGESF 124
Cdd:TIGR01997  81 RTTLRQGFKFNNPNATSACGCGESF 105
IscA TIGR02011
iron-sulfur cluster assembly protein IscA; This model represents the IscA component of the ISC ...
 23-124 1.35e-31

iron-sulfur cluster assembly protein IscA; This model represents the IscA component of the ISC system for iron-sulfur cluster assembly. The ISC system consists of IscRASU, HscAB and an Isc-specific ferredoxin. IscA previously was believed to act as a scaffold and now is seen as an iron donor protein. This clade is limited to the proteobacteria. [Biosynthesis of cofactors, prosthetic groups, and carriers, Other]


Pssm-ID: 213674  Cd Length: 105  Bit Score: 108.37  E-value: 1.35e-31
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15227287    23 LTLTDEAASRVHHLLQQRQKPF-LRLGVKARGCNGLSYTLNYADEKGKFDELVEEKGVRILVEPKALMHVIGTKMDFVDD 101
Cdd:TIGR02011   1 ITLTDSAAARVNTFLANRGKGFgLRLGVKTSGCSGMAYVLEFVDEPTPDDIVFEDKGVKIVIDGKSLQYLDGTQLDFVKE 80

                          90       100
                  ....*....|....*....|...
gi 15227287   102 KLRSEFVFINPNSQGQCGCGESF 124
Cdd:TIGR02011  81 GLNEGFKFTNPNVKDECGCGESF 103
iscA PRK09502
iron-sulfur cluster assembly protein IscA;
23-124 1.78e-30

iron-sulfur cluster assembly protein IscA;


Pssm-ID: 181914 [Multi-domain]  Cd Length: 107  Bit Score: 105.34  E-value: 1.78e-30
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15227287   23 LTLTDEAASRVHHLLQQRQKPF-LRLGVKARGCNGLSYTLNYADEKGKFDELVEEKGVRILVEPKALMHVIGTKMDFVDD 101
Cdd:PRK09502   3 ITLSDSAAARVNTFLANRGKGFgLRLGVRTSGCSGMAYVLEFVDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKE 82

                         90       100
                 ....*....|....*....|...
gi 15227287  102 KLRSEFVFINPNSQGQCGCGESF 124
Cdd:PRK09502  83 GLNEGFKFTNPNVKDECGCGESF 105
sufA PRK09504
iron-sulfur cluster assembly scaffold protein; Provisional
 21-124 3.63e-26

iron-sulfur cluster assembly scaffold protein; Provisional


Pssm-ID: 181915  Cd Length: 122  Bit Score: 94.80  E-value: 3.63e-26
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15227287   21 QVLTLTDEAASRVHHLLQQR-QKPFLRLGVKARGCNGLSYTLNYADEKGKFDELVEEKGVRILVEPKALMHVIGTKMDFV 99
Cdd:PRK09504  16 QGLTLTPAAAAHIRELMAKQpGMKGVRLGVKQTGCAGFGYVLDSVSEPDKDDLVFEHDGAKLFVPLQAMPFIDGTEVDYV 95

                         90       100
                 ....*....|....*....|....*
gi 15227287  100 DDKLRSEFVFINPNSQGQCGCGESF 124
Cdd:PRK09504  96 REGLNQIFKFHNPKAQNECGCGESF 120
PRK13623 PRK13623
iron-sulfur cluster insertion protein ErpA; Provisional
 23-124 2.91e-20

iron-sulfur cluster insertion protein ErpA; Provisional


Pssm-ID: 184186  Cd Length: 115  Bit Score: 79.58  E-value: 2.91e-20
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15227287   23 LTLTDEAASRVHHLLQQRQKPFLRLGV--KARGCNGLSYTLNYADEKGKFDELVEEKGVRILVEPKALMHVIGTKMDFVD 100
Cdd:PRK13623  10 LVFTDAAAAKVKELIEEEGNPDLKLRVyiTGGGCSGFQYGFTFDEQVNEDDTTIEKQGVTLVVDPMSLQYLVGAEVDYTE 89

                         90       100
                 ....*....|....*....|....
gi 15227287  101 DKLRSEFVFINPNSQGQCGCGESF 124
Cdd:PRK13623  90 GLEGSRFVIKNPNAKTTCGCGSSF 113
PLN03082 PLN03082
Iron-sulfur cluster assembly; Provisional
 21-125 3.94e-15

Iron-sulfur cluster assembly; Provisional


Pssm-ID: 215564  Cd Length: 163  Bit Score: 67.64  E-value: 3.94e-15
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15227287   21 QVLTLTDEAASRVHHLLQQR---QKPFLRLGVKARGCNGLSYTLNYADEKGKFDELVEEKGVRILVEPKALMHVIGTKMD 97
Cdd:PLN03082  53 DAVHMTDNCIRRLKELQTSEpsaEDKMLRLSVETGGCSGFQYVFELDDKTNSDDRVFEKDGVKLVVDNISYDFVKGATVD 132

                         90       100
                 ....*....|....*....|....*....
gi 15227287   98 FVDDKLRSEFVF-INPNSQGQCGCGESFM 125
Cdd:PLN03082 133 YVEELIRSAFVVsTNPSAVGGCSCKSSFM 161
Fe-S_biosyn pfam01521
Iron-sulphur cluster biosynthesis; This family is involved in iron-sulphur cluster ...
 23-122 1.84e-14

Iron-sulphur cluster biosynthesis; This family is involved in iron-sulphur cluster biosynthesis. Its members include proteins that are involved in nitrogen fixation such as the HesB and HesB-like proteins.


Pssm-ID: 426304  Cd Length: 111  Bit Score: 64.59  E-value: 1.84e-14
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15227287    23 LTLTDEAASRVHHLLQQRQKPfLRLGVK------ARGC--NGLSYTLNYADEK-GKFDELVEEKGVRILVEPKALMHV-I 92
Cdd:pfam01521   3 ITITDAAAERLKKLLAGDKKE-LRLDVDdgggpySKGGcsIGGKFSLVLVDEPdPDYDEVIESNGGPIYVDSYSLPFLdE 81

                          90       100       110
                  ....*....|....*....|....*....|
gi 15227287    93 GTKMDFVDDKLRSEFVFINPNSQGQCGCGE 122
Cdd:pfam01521  82 GLTLDFVEDLGTLGLKSDNGNLDGNVGCGD 111
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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