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Conserved domains on  [gi|15219441|ref|NP_177476|]
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drought-repressed 4 [Arabidopsis thaliana]

Protein Classification

STI domain-containing protein( domain architecture ID 10648611)

STI domain-containing protein

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
STI smart00452
Soybean trypsin inhibitor (Kunitz) family of protease inhibitors;
28-192 2.83e-56

Soybean trypsin inhibitor (Kunitz) family of protease inhibitors;


:

Pssm-ID: 214670  Cd Length: 172  Bit Score: 175.98  E-value: 2.83e-56
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15219441     28 VEDSVGRLLRPGQTYHIVPANPETGGGIFSNSE--EICPLDIFQSNNPLDLGLPIKFKS---ELWFVKEMNSITIEFEAP 102
Cdd:smart00452   1 VLDTDGNPLRNGGTYYILPAIRGHGGGLTLAATgnEICPLTVVQSPNEVDNGLPVKFSPpnpSDFIIRESTDLNIEFDAP 80

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15219441    103 nwFLCPKeSKGWRVVYSEeFKKSLIISTGGSSNP--SGFQIHRVDGG--AYKIVYCTNIS-TTTCMNVGIFTDISGARRL 177
Cdd:smart00452  81 --PLCAQ-STVWTVDEDS-TPGGLAVKTGGYPGVndSWFKIEKYSGEsnGYKLVYCPNGSdDDKCGDVGIFIDPNGGRRL 156

                          170
                   ....*....|....*
gi 15219441    178 ALTSDEALLVKFQKA 192
Cdd:smart00452 157 VLSNENPLVVVFKKA 171
 
Name Accession Description Interval E-value
STI smart00452
Soybean trypsin inhibitor (Kunitz) family of protease inhibitors;
28-192 2.83e-56

Soybean trypsin inhibitor (Kunitz) family of protease inhibitors;


Pssm-ID: 214670  Cd Length: 172  Bit Score: 175.98  E-value: 2.83e-56
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15219441     28 VEDSVGRLLRPGQTYHIVPANPETGGGIFSNSE--EICPLDIFQSNNPLDLGLPIKFKS---ELWFVKEMNSITIEFEAP 102
Cdd:smart00452   1 VLDTDGNPLRNGGTYYILPAIRGHGGGLTLAATgnEICPLTVVQSPNEVDNGLPVKFSPpnpSDFIIRESTDLNIEFDAP 80

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15219441    103 nwFLCPKeSKGWRVVYSEeFKKSLIISTGGSSNP--SGFQIHRVDGG--AYKIVYCTNIS-TTTCMNVGIFTDISGARRL 177
Cdd:smart00452  81 --PLCAQ-STVWTVDEDS-TPGGLAVKTGGYPGVndSWFKIEKYSGEsnGYKLVYCPNGSdDDKCGDVGIFIDPNGGRRL 156

                          170
                   ....*....|....*
gi 15219441    178 ALTSDEALLVKFQKA 192
Cdd:smart00452 157 VLSNENPLVVVFKKA 171
Kunitz_legume pfam00197
Trypsin and protease inhibitor;
28-191 2.55e-48

Trypsin and protease inhibitor;


Pssm-ID: 395144  Cd Length: 174  Bit Score: 155.92  E-value: 2.55e-48
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15219441    28 VEDSVGRLLRPGQTYHIVPANP-ETGGGI--FSNSEEICPLDIFQSNNPLDLGLPIKF---KSELWFVKEMNSITIEFEA 101
Cdd:pfam00197   1 VLDTDGNPLRAGVEYYILPAIGgGSGGGLtlASRGNGTCPLDVVQEPSEVSKGLPVKFspsNSKKGVIRESTDLNIEFFS 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15219441   102 PNWfLCPkESKGWRVVYSEEFKKSLIISTGG-------SSNPSGFQIHRVdGGAYKIVYC-TNISTTTCMNVGIFTDISG 173
Cdd:pfam00197  81 APT-ICV-QSTVWKVGDGDPETGRRFVVTGGvvgnpgpDTVSNWFKIEKT-GGGYKLVFCpSVCCKVKCGDVGIFVDDNG 157

                         170
                  ....*....|....*...
gi 15219441   174 ARRLALtSDEALLVKFQK 191
Cdd:pfam00197 158 NRRLAL-SDEPFPVVFKK 174
beta-trefoil_STI cd00178
soybean trypsin inhibitor (STI)-like domain, beta-trefoil fold; The STI-like domain is found ...
 39-191 1.06e-43

soybean trypsin inhibitor (STI)-like domain, beta-trefoil fold; The STI-like domain is found in the soybean trypsin inhibitor (Kunitz) family of protease inhibitors. They inhibit proteases by binding with high affinity to their active sites. Plant Kunitz-type inhibitors are thought to be important in defense, especially against insect pests. The STI-like domain shows a beta-trefoil fold, which is characterized by 12 beta strands folded into three similar trefoil subdomains (alpha, beta, and gamma) associated to give an overall structure with pseudo-3-fold symmetry.


Pssm-ID: 467384  Cd Length: 161  Bit Score: 143.65  E-value: 1.06e-43
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15219441  39 GQTYHIVPANPETGGGI--FSNSEEICPLDIFQSNNPLDLGLPIKFKS---ELWFVKEMNSITIEFEAPNWflCPKESKG 113
Cdd:cd00178   1 GVPYYILPAIWGGGGGLtlAKTGNETCPLDVVQSPSDTDNGLPVTFSPanpKDGVIRESTDLNIKFSADTT--CCAESTV 78

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15219441 114 WRVVYSEEFKKSLiISTGGSSN----PSGFQIHRV-DGGAYKIVYCTNIS--TTTCMNVGIFTDiSGARRLALTSDEALL 186
Cdd:cd00178  79 WKVVGDDSTGGRF-VTTGGVKGnetlNSWFKIEKAgNGNGYKLVFCPSVCgcKVVCGDVGIVVD-NGNRRLALTEGEPLE 156


                ....*
gi 15219441 187 VKFQK 191
Cdd:cd00178 157 VVFKK 161
 
Name Accession Description Interval E-value
STI smart00452
Soybean trypsin inhibitor (Kunitz) family of protease inhibitors;
28-192 2.83e-56

Soybean trypsin inhibitor (Kunitz) family of protease inhibitors;


Pssm-ID: 214670  Cd Length: 172  Bit Score: 175.98  E-value: 2.83e-56
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15219441     28 VEDSVGRLLRPGQTYHIVPANPETGGGIFSNSE--EICPLDIFQSNNPLDLGLPIKFKS---ELWFVKEMNSITIEFEAP 102
Cdd:smart00452   1 VLDTDGNPLRNGGTYYILPAIRGHGGGLTLAATgnEICPLTVVQSPNEVDNGLPVKFSPpnpSDFIIRESTDLNIEFDAP 80

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15219441    103 nwFLCPKeSKGWRVVYSEeFKKSLIISTGGSSNP--SGFQIHRVDGG--AYKIVYCTNIS-TTTCMNVGIFTDISGARRL 177
Cdd:smart00452  81 --PLCAQ-STVWTVDEDS-TPGGLAVKTGGYPGVndSWFKIEKYSGEsnGYKLVYCPNGSdDDKCGDVGIFIDPNGGRRL 156

                          170
                   ....*....|....*
gi 15219441    178 ALTSDEALLVKFQKA 192
Cdd:smart00452 157 VLSNENPLVVVFKKA 171
Kunitz_legume pfam00197
Trypsin and protease inhibitor;
28-191 2.55e-48

Trypsin and protease inhibitor;


Pssm-ID: 395144  Cd Length: 174  Bit Score: 155.92  E-value: 2.55e-48
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15219441    28 VEDSVGRLLRPGQTYHIVPANP-ETGGGI--FSNSEEICPLDIFQSNNPLDLGLPIKF---KSELWFVKEMNSITIEFEA 101
Cdd:pfam00197   1 VLDTDGNPLRAGVEYYILPAIGgGSGGGLtlASRGNGTCPLDVVQEPSEVSKGLPVKFspsNSKKGVIRESTDLNIEFFS 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15219441   102 PNWfLCPkESKGWRVVYSEEFKKSLIISTGG-------SSNPSGFQIHRVdGGAYKIVYC-TNISTTTCMNVGIFTDISG 173
Cdd:pfam00197  81 APT-ICV-QSTVWKVGDGDPETGRRFVVTGGvvgnpgpDTVSNWFKIEKT-GGGYKLVFCpSVCCKVKCGDVGIFVDDNG 157

                         170
                  ....*....|....*...
gi 15219441   174 ARRLALtSDEALLVKFQK 191
Cdd:pfam00197 158 NRRLAL-SDEPFPVVFKK 174
beta-trefoil_STI cd00178
soybean trypsin inhibitor (STI)-like domain, beta-trefoil fold; The STI-like domain is found ...
 39-191 1.06e-43

soybean trypsin inhibitor (STI)-like domain, beta-trefoil fold; The STI-like domain is found in the soybean trypsin inhibitor (Kunitz) family of protease inhibitors. They inhibit proteases by binding with high affinity to their active sites. Plant Kunitz-type inhibitors are thought to be important in defense, especially against insect pests. The STI-like domain shows a beta-trefoil fold, which is characterized by 12 beta strands folded into three similar trefoil subdomains (alpha, beta, and gamma) associated to give an overall structure with pseudo-3-fold symmetry.


Pssm-ID: 467384  Cd Length: 161  Bit Score: 143.65  E-value: 1.06e-43
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15219441  39 GQTYHIVPANPETGGGI--FSNSEEICPLDIFQSNNPLDLGLPIKFKS---ELWFVKEMNSITIEFEAPNWflCPKESKG 113
Cdd:cd00178   1 GVPYYILPAIWGGGGGLtlAKTGNETCPLDVVQSPSDTDNGLPVTFSPanpKDGVIRESTDLNIKFSADTT--CCAESTV 78

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15219441 114 WRVVYSEEFKKSLiISTGGSSN----PSGFQIHRV-DGGAYKIVYCTNIS--TTTCMNVGIFTDiSGARRLALTSDEALL 186
Cdd:cd00178  79 WKVVGDDSTGGRF-VTTGGVKGnetlNSWFKIEKAgNGNGYKLVFCPSVCgcKVVCGDVGIVVD-NGNRRLALTEGEPLE 156


                ....*
gi 15219441 187 VKFQK 191
Cdd:cd00178 157 VVFKK 161
beta-trefoil_STI_CrataBL-like cd23374
soybean trypsin inhibitor (STI)-like domain, beta-trefoil fold, found in Crateva tapia bark ...
 35-191 4.98e-39

soybean trypsin inhibitor (STI)-like domain, beta-trefoil fold, found in Crateva tapia bark lectin (CrataBL) and similar proteins; CrataBL is both a Kunitz-type plant protease inhibitor and a glucose- and N-acetylglucosamine-binding lectin. It has hemagglutinating activity against human and rabbit erythrocytes which does not require divalent cations. It inhibits factor Xa and, to a lesser extent, trypsin. It does not inhibit neutrophil elastase, human plasma kallikrein, papain, human plasmin, porcine pancreatic kallikrein and bovine chymotrypsin. CrataBL has insecticidal activity against the termite species Nasutitermes corniger. It induces apoptosis in prostate cancer cell lines DU145 and PC3. The family includes CrataBL-form I and CrataBL-form II. This subfamily also includes Arabidopsis thaliana Kunitz trypsin inhibitor 3 (AtKTI3) that exhibits Kunitz trypsin protease inhibitor activity. Members of this subfamily contain an STI-like domain with a beta-trefoil fold, which is characterized by 12 beta strands folded into three similar trefoil subdomains (alpha, beta, and gamma) associated to give an overall structure with pseudo-3-fold symmetry.


Pssm-ID: 467399  Cd Length: 160  Bit Score: 131.84  E-value: 4.98e-39
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15219441  35 LLRPGQTYHIVPANPETGGGIF-----SNSEEICPLDIFQSNNPLDLGLPIKFK---SELWFVKEMNSITIEFEAPNWFl 106
Cdd:cd23374   1 PVLAGVPYYILPAKIGTGGGLIpsnrrKNTQQLCPLDIVQSQFPFVLGVPVTFTplnSKLKVVPLSTNLNIEFDSDVWL- 79

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15219441 107 CPkESKGWRVVYSEEFKKSlIISTGG--SSNPSGFQIHRvDGGAYKIVYCTNisTTTCMNVGIFTDISGARRLALtSDEA 184
Cdd:cd23374  80 CP-ESKVWTVDSSQWLRGS-YVSTGGekGSGGSWFRIER-DGDSYKLVHCPR--GTSCRDVGIETDGGGVRRLVL-GDRP 153


                ....*..
gi 15219441 185 LLVKFQK 191
Cdd:cd23374 154 LAVKFQK 160
beta-trefoil_STI_VvMLP-like cd23375
soybean trypsin inhibitor (STI)-like domain, beta-trefoil fold, found in Vitis vinifera ...
 28-191 2.18e-30

soybean trypsin inhibitor (STI)-like domain, beta-trefoil fold, found in Vitis vinifera miraculin-like protein (VvMLP) and similar proteins; This subfamily includes VvMLP, Synsepalum dulcificum miraculin (SdMIR), and Arabidopsis thaliana Kunitz trypsin inhibitor 5 (AtKTI5, also known as AtKTI2). VvMLP exhibits significant homology to miraculin. However, it exists as a monomer in solution with no detectable taste-modifying activity. It can act as a moderate trypsin inhibitor. SdMIR has the property of modifying a sour taste into a sweet taste. This alteration of taste perception persists for many minutes. AtKTI5 can inhibit both serine proteases and cysteine proteases. It may be involved in the modulation of the proteases that participate in the hydrolysis of dietary proteins in the gut of spider mites. Members of this subfamily contain an STI-like domain with a beta-trefoil fold, which is characterized by 12 beta strands folded into three similar trefoil subdomains (alpha, beta, and gamma) associated to give an overall structure with pseudo-3-fold symmetry.


Pssm-ID: 467400  Cd Length: 177  Bit Score: 110.00  E-value: 2.18e-30
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15219441  28 VEDSVGRLLRPGQTYHIVPANPETGGGI--FSNSEEICPLDIFQSNNPLDLGLPIKF---KSELWFVKEMNSITIEFEAP 102
Cdd:cd23375   5 VLDTAGKILRTGVNYYILPVNRGRGGGLtlASTGNETCPLDVVQEQNEVNNGLPLTFspvNPKKGVIRVSTDLNIKFSAS 84

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15219441 103 NwfLCPkESKGWRVVYSEEFKKSLIISTGGS-SNP------SGFQIHRVDGGaYKIVYC---TNISTTTCMNVGIFTDiS 172
Cdd:cd23375  85 T--SCP-ESTVWKLDDYDESTGQYFVTTGGVeGNPgretirNWFKIEKYEDG-YKLVYCpsvCNYCKVICKDVGIYID-N 159

                       170
                ....*....|....*....
gi 15219441 173 GARRLALtSDEALLVKFQK 191
Cdd:cd23375 160 GVRRLAL-SDKPLKVKFKK 177
beta-trefoil_STI_WCI3-like cd23362
soybean trypsin inhibitor (STI)-like domain, beta-trefoil fold, found in Psophocarpus ...
 28-193 1.06e-28

soybean trypsin inhibitor (STI)-like domain, beta-trefoil fold, found in Psophocarpus tetragonolobus chymotrypsin inhibitor 3 (WCI-3) and similar proteins; This subfamily includes Psophocarpus tetragonolobus WCI-3, trypsin inhibitor 1 (WTI-1), and trypsin inhibitor DE-3 from Erythrina caffra (ETI). WTI-1 is a Kunitz type protease inhibitor that inhibits bovine trypsin stoichiometrically, but not bovine alpha-chymotrypsin. WCI-3 is a Kunitz-type winged bean chymotrypsin inhibitor (WbCI) that inhibits alpha-chymotrypsin at the molar ratio of 1:2 instead of 1:1, the usual ratio of 1:1 common to other members of the family. ETI is a trypsin inhibitor that shows high homology to other Kunitz trypsin protease inhibitors, but has the unique ability to bind and inhibit tissue plasminogen activator. Members of this subfamily contain an STI-like domain with a beta-trefoil fold, which is characterized by 12 beta strands folded into three similar trefoil subdomains (alpha, beta, and gamma) associated to give an overall structure with pseudo-3-fold symmetry.


Pssm-ID: 467387  Cd Length: 170  Bit Score: 105.39  E-value: 1.06e-28
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15219441  28 VEDSVGRLLRPGQTYHIVPANPETGGGI--FSNSEEICPLDIFQSNNPLDLGLPIKFKSELW--FVKEMNSITIEFEAPN 103
Cdd:cd23362   2 VVDTDGNPVENGGTYYILPVIWGKGGGIelAATGNETCPLTVVQSPNEVSKGLPIRISSPLRiaFIPEGLLLRIGFTAVP 81

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15219441 104 WflCPKESKGWRVVYSEEFKKSLIISTGGSSNPSGFQIHRV--DGGAYKIVYCTNiSTTTCMNVGIFTDISGARRLALTS 181
Cdd:cd23362  82 P--CAPTPSWWTVVKGLPEGPAVKLTGYKNTVDGWFKIEKVssDLNSYKLLFCPE-DDDSCGDIGIHRDDKGNRRLVVTE 158

                       170
                ....*....|..
gi 15219441 182 DEALLVKFQKAA 193
Cdd:cd23362 159 ENPLVVVFQKAE 170
beta-trefoil_STI_WSCP_II cd23360
soybean trypsin inhibitor (STI)-like domain, beta-trefoil fold, found in class II ...
 27-191 2.28e-27

soybean trypsin inhibitor (STI)-like domain, beta-trefoil fold, found in class II water-soluble chlorophyll proteins (WSCPs) and similar proteins; There are two kinds of water-soluble chlorophyll (Chl) proteins (WSCPs): Chenopodium-type (Class I, a WSCP from Chenopodium, Atriplex, Polygonum, and Amaranthus species) and Brassica-type (Class II, a WSCP from Brassica, Raphanus, and Lepidium species). Classes I and II WSCPs differ mainly in their photoconvertiblity. Class I WSCPs show a light-induced absorption change, whereas Class II WSCPs do not. This family includes Class II WSCPs. They possess the complete motif of the Kunitz-type proteinase inhibitor but may not inhibit trypsin, whose activity is inhibited strongly by one Kunitz-proteinase inhibitor, the soybean trypsin inhibitor (STI). Members of this subfamily contain a STI-like domain with a beta-trefoil fold, which is characterized by 12 beta strands folded into three similar trefoil subdomains (alpha, beta, and gamma) associated to give an overall structure with pseudo-3-fold symmetry.


Pssm-ID: 467385  Cd Length: 176  Bit Score: 102.14  E-value: 2.28e-27
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15219441  27 HVEDSVGRLLRPGQTYHIVPANPETGGGIFSNSEEI---CPLDIFQSNNPLDLGLPIKFKSELW----FVKEMNSITIEF 99
Cdd:cd23360   2 PVKDTAGNPLKTGAQYFIQPVKTNNGGGLVPAAIDLlplCPLGITQTLLPYQPGLPVSFSLPLSsvgnTVRTSTDVNIEF 81

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15219441 100 EAPNWFLCPKESKGWRVVYSEEFKKSLIISTGGS--SNPSGFQIHRVDGGA--YKIVyctnisTTTCMNVGIFTDI-SGA 174
Cdd:cd23360  82 KSPIWPVCKEFSKLWAVDSSSSAPKEPAIIIGGKpgSRNSLFKIEKAGGGAntYKLT------TLDGGTVGTIPGPwLGA 155

                       170
                ....*....|....*....
gi 15219441 175 RRLALTSDEA--LLVKFQK 191
Cdd:cd23360 156 PQLVLTNDDAktLLVKFRK 174
beta-trefoil_STI_AtTPI-like cd23366
soybean trypsin inhibitor (STI)-like domain, beta-trefoil fold, found in Arabidopsis thaliana ...
 11-192 6.64e-27

soybean trypsin inhibitor (STI)-like domain, beta-trefoil fold, found in Arabidopsis thaliana trypsin protease inhibitor (AtTPI) and similar proteins; AtTPI, also called kunitz trypsin inhibitor 4 (AtKTI4), or Kunitz trypsin inhibitor 1 (AtKTI1), exhibits Kunitz trypsin protease inhibitor activity. It is involved in modulating programmed cell death (PCD) in plant-pathogen interactions. It can also inhibit both serine proteases and cysteine proteases. It may be involved in the modulation of proteases that participate in the hydrolysis of dietary proteins in the gut of spider mites. Members of this subfamily contain an STI-like domain with a beta-trefoil fold, which is characterized by 12 beta strands folded into three similar trefoil subdomains (alpha, beta, and gamma) associated to give an overall structure with pseudo-3-fold symmetry.


Pssm-ID: 467391  Cd Length: 195  Bit Score: 101.36  E-value: 6.64e-27
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15219441  11 FLVVALAAPSLARPDNH--VEDSVGRLLrPGQTYHIVPANPETGGG---IFSNSEEICPLDIFQSNNPLDLGLPIKF--- 82
Cdd:cd23366   4 YFLLALTAVLAATANAGgaVLDSDGDII-FNGSYYVLPVIRGTGGGgltLSGLGSEPCPLYVGQESSEVNEGIPVKFsnw 82

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15219441  83 KSELWFVKEMNSITIEFEAPNwFLCPKeSKGWRVVYSEEFKKSLIISTG-------GSSNpSGFQIHRVDG--GAYKIVY 153
Cdd:cd23366  83 KSKVGFVPESENLNIEMDVGA-TICIQ-STYWWLGEFDKERKALFVAAGpkpegfkDSLK-SFFQIKKSEDllGGYKIVF 159

                       170       180       190
                ....*....|....*....|....*....|....*....
gi 15219441 154 CTNisTTTCMNVGIFTDISGARRLALtSDEALLVKFQKA 192
Cdd:cd23366 160 CPS--DPSCTDVGIFVDENGVRRLAL-SDKPFEVVFVKA 195
beta-trefoil_STI_MkMLP-like cd23370
soybean trypsin inhibitor (STI)-like domain, beta-trefoil fold, found in Murraya koenigii ...
 28-191 3.69e-25

soybean trypsin inhibitor (STI)-like domain, beta-trefoil fold, found in Murraya koenigii miraculin-like protein (MkMLP) and similar proteins; This subfamily includes Theobroma cacao 21 kDa seed protein (TcASP) and Murraya koenigii miraculin-like protein (MkMLP). TcASP shows homology to the soybean trypsin inhibitor (Kunitz) family of protease inhibitors. MkMLP is closer to miraculin, a taste modifying protein, rather than classical Kunitz family members like soybean Kunitz-type trypsin inhibitor (STI). MkMLP is functionally unstable at higher temperatures. Members of this subfamily contain an STI-like domain with a beta-trefoil fold, which is characterized by 12 beta strands folded into three similar trefoil subdomains (alpha, beta, and gamma) associated to give an overall structure with pseudo-3-fold symmetry.


Pssm-ID: 467395  Cd Length: 179  Bit Score: 96.62  E-value: 3.69e-25
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15219441  28 VEDSVGRLLRPGQTYHIVPANPETGGG---IFSNSEEICPLDIFQSNNPLDLGLPIKFK---SELWFVKEMNSITIEFEA 101
Cdd:cd23370   2 VLDINGNKVRTGTEYYIVSAIWGAGGGglsLFRGRNGTCPLDVIQLRSDLDRGLPLTFSpadYNDGVVYESTDLNIKFSA 81

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15219441 102 PNwFLCPkESKGWRV-VYSEEFKKSLIISTGGSSNPSG------FQIHRVDGG-AYKIVYCTNI---STTTCMNVGIFTD 170
Cdd:cd23370  82 AD-ALCN-ESTVWKVdNYDESTGKWFITTGGVEGNPGAqtllnwFKIEKVGTGnTYKIVHCPSVcdsCVTLCNDVGRSSD 159

                       170       180
                ....*....|....*....|.
gi 15219441 171 iSGARRLALTSDEALLVKFQK 191
Cdd:cd23370 160 -DGVRRLALSDDPPFPVVFIK 179
beta-trefoil_STI_SKTI cd23363
soybean trypsin inhibitor (STI)-like domain, beta-trefoil fold, found in the soybean Kunitz ...
 26-191 1.57e-24

soybean trypsin inhibitor (STI)-like domain, beta-trefoil fold, found in the soybean Kunitz trypsin inhibitor (SKTI) subfamily; SKTI is extracted from soybean (Glycine max L.) seeds. It shows inhibition of trypsin and possesses insect resistance and anti-tumor properties. This subfamily includes KTI1-3. KTI1 and KTI2 probably do not possess trypsin inhibitor activity. KTI3, also called trypsin inhibitor A, is responsible for most of the Kunitz trypsin inhibitor activity and protein found in soybean seeds. Members of this subfamily contain a soybean trypsin inhibitor (STI)-like domain with a beta-trefoil fold, which is characterized by 12 beta strands folded into three similar trefoil subdomains (alpha, beta, and gamma) associated to give an overall structure with pseudo-3-fold symmetry.


Pssm-ID: 467388  Cd Length: 175  Bit Score: 94.84  E-value: 1.57e-24
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15219441  26 NHVEDSVGRLLRPGQTYHIVPANPETGGGIFSNS--EEICPLDIFQSNNPLDLGLPIKFKSEL--WFVKEMNSITIEFEA 101
Cdd:cd23363   1 DFVLDTDGNPLQNGGTYYVLPVIRGAGGGIRVAPtgNERCPLTVVQSRNELDKGIGTIISSPYriRFIAEGHPLSIKFDS 80

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15219441 102 -PNWFLCPKESKGWRVVysEEFKKSLIISTGGSSNP-SG-FQIHRVDGGA---YKIVYCTN-ISTTTCMNVGIFTDISGA 174
Cdd:cd23363  81 fAVIPLCVPIPTEWSVV--EDLPEGPAVKIGENKNAvDGwFRIERVSDDEfngYKLVFCPQqAEDDKCGDIGISIDDDGI 158

                       170
                ....*....|....*..
gi 15219441 175 RRLALTSDEALLVKFQK 191
Cdd:cd23363 159 RRLVVSKNKPLVVQFQK 175
beta-trefoil_STI_DrTI cd23376
soybean trypsin inhibitor (STI)-like domain, beta-trefoil fold, found in Delonix regia ...
 28-189 1.69e-24

soybean trypsin inhibitor (STI)-like domain, beta-trefoil fold, found in Delonix regia Kunitz-type serine protease inhibitor DrTI and similar proteins; DrTI is a Kunitz-type trypsin inhibitor that inhibits bovine trypsin and human plasma kallikrein, but not chymotrypsin and tissue kallikrein. Members of this subfamily contain an STI-like domain with a beta-trefoil fold, which is characterized by 12 beta strands folded into three similar trefoil subdomains (alpha, beta, and gamma) associated to give an overall structure with pseudo-3-fold symmetry.


Pssm-ID: 467401  Cd Length: 174  Bit Score: 94.70  E-value: 1.69e-24
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15219441  28 VEDSVGRLLRPGQTYHIVPANP-ETGGGIFSNSEEI--CPLDIFQSNNPLDLGLPIKFKselwfVKEMNS--------IT 96
Cdd:cd23376   3 VKDTNGNPLSPGAEYYILPANSgPGGGGLRLGKTGNstCPLTVLQEYSELFLGLPVKFN-----VQGSSDgiiltgtpLD 77

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15219441  97 IEFEA-PNwflCPKESKgWRVVYsEEFKKSLIISTGGSSNPSG-------FQIHRVDGGaYKIVYCTNISTTTCMNVGIF 168
Cdd:cd23376  78 IEFVEkPD---CAESSK-WVVVK-DDFYPTKWVGIGGGEDHPGkeivdgvFKIEKYGEG-YKLVFCPKGSSGTCFDIGRY 151

                       170       180
                ....*....|....*....|..
gi 15219441 169 TDISGaRRLALTSD-EALLVKF 189
Cdd:cd23376 152 DDVNG-RRLVLENSgTPFEVVF 172
beta-trefoil_STI_SPOR cd23368
soybean trypsin inhibitor (STI)-like domain, beta-trefoil fold, found in Ipomoea batatas ...
 28-191 1.40e-22

soybean trypsin inhibitor (STI)-like domain, beta-trefoil fold, found in Ipomoea batatas sporamin and similar proteins; This subfamily includes Ipomoea batatas sporamin A and sporamin B. They are major tuberous root proteins that belong to the soybean trypsin inhibitor (Kunitz) family of protease inhibitors. They exhibit antitumor activity in a number of types of tumor cells. Members of this subfamily contain an STI-like domain with a beta-trefoil fold, which is characterized by 12 beta strands folded into three similar trefoil subdomains (alpha, beta, and gamma) associated to give an overall structure with pseudo-3-fold symmetry.


Pssm-ID: 467393  Cd Length: 174  Bit Score: 89.72  E-value: 1.40e-22
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15219441  28 VEDSVGRLLRPGQTYHIVPANPETGGGIFS----NSEEICPLDIFQSNNpLDLGLPIKFKSE---LWFVKEMNSITIEFE 100
Cdd:cd23368   3 VLDTDGDELRAGGTYYITSATWGAGGGGVRlvrlDSTTKCPSDVIISRS-LDDGDPITITPAdpnATVVLPSTFQSFKFN 81

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15219441 101 APNWFLCPKESKgWRVVYSEEFKKSLIISTGGSSNPSG-FQIHRVDGG--AYKIVYCtNISTTTCMNVGIFTDIS-GARR 176
Cdd:cd23368  82 IPTNPLCVNNVY-WGIQYDPESGQYFVKAGEFVSNNSNqFKIEVVPDNlnAYKITYC-PFGSDKCYNVGTYYDPLlRATR 159

                       170
                ....*....|....*
gi 15219441 177 LALTSDEALLVKFQK 191
Cdd:cd23368 160 LALSDDSPFVVVFKK 174
beta-trefoil_STI_MP4-like cd23377
soybean trypsin inhibitor (STI)-like domain, beta-trefoil fold, found in Mucuna pruriens MP-4 ...
 28-192 1.13e-20

soybean trypsin inhibitor (STI)-like domain, beta-trefoil fold, found in Mucuna pruriens MP-4 and similar proteins; This subfamily includes Mucuna pruriens MP-4, Canavalia lineata subtilisin inhibitor CLSI-II, Cicer arietinum trypsin protein inhibitor 2 (CaTI2), Pisum sativum Kunitz-type trypsin inhibitor-like 1 protein (PIP20-1) and Kunitz-type trypsin inhibitor-like 2 protein (PIP20-2). MP-4 contributes significantly to the snake venom neutralization activity of Mucuna pruriens seeds through an indirect antibody-mediated mechanism and not through direct inhibition of venom proteases. CLSI-II inhibits subtilisin-type microbial serine proteases including proteinase K, subtilisin BPN', subtilisin Carlsberg and subtilisin E in a non-stoichiometric manner. It weakly inhibits Aspergillus oryzae protease and some metalloproteases including pronase E. It does not inhibit trypsin, chymotrypsin, Streptomyces griseus alkaline protease or Achromobacter lyticus lysyl endopeptidase. CLSI-II has a wider inhibitory specificity than CLSI-III. CaTI2 is a Kunitz trypsin inhibitor (KTI) with antifungal effect on Fusarium oxysporum f. sp. ciceris, a fungal pathogen known to cause severe damage to chickpea crop. It may be binding to the trypsin active pocket in a non-substrate like manner. PIP20-1 (also called protease inhibitor from pea 1 or FUC1) and PIP20-2 (also called protease inhibitor from pea 2 or FUC2) may act as protease inhibitors involved in plant defense responses. Members of this subfamily contain an STI-like domain with a beta-trefoil fold, which is characterized by 12 beta strands folded into three similar trefoil subdomains (alpha, beta, and gamma) associated to give an overall structure with pseudo-3-fold symmetry.


Pssm-ID: 467402  Cd Length: 179  Bit Score: 84.63  E-value: 1.13e-20
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15219441  28 VEDSVGRLLRPGQTYHIVPANPE-TGGGI----FSNSEeiCPLDIFQSNNPLDLGLPIKFKSELWFVKEM---NSITIEF 99
Cdd:cd23377   4 VRDTNGNPIFPGGRYYIMPAIFGpAGGGVklgkTGNST--CPVTVLQDYSEVVNGLPVKFTIPGISPGIIftgTPLDIEF 81

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15219441 100 EAPNWflCPKESKgWRVVYSEEFKKSLIiSTGGSSNPSGFQI---------HRVdGGAYKIVYCTNIS-TTTCMNVGIFT 169
Cdd:cd23377  82 TKKPN--CAESSK-WLVFVDDFIPKACV-GIGGPEDHPGKQIlsgkfniqkYGS-GNGYKLVFCPDGSaPGNCSDIGRYD 156

                       170       180
                ....*....|....*....|...
gi 15219441 170 DISGARRLALTSDEALLVKFQKA 192
Cdd:cd23377 157 NEEGGRRLVLTEDEPFEVVFVDA 179
beta-trefoil_STI_LlTI-like cd23365
soybean trypsin inhibitor (STI)-like domain, beta-trefoil fold, found in Leucaena leucocephala ...
 28-192 9.90e-20

soybean trypsin inhibitor (STI)-like domain, beta-trefoil fold, found in Leucaena leucocephala trypsin inhibitor (LlTI) and similar proteins; LlTI is a Kunitz-type trypsin inhibitor that inhibits trypsin, plasmin, human plasma kallikrein, chymotrypsin, and factor XIIa activity. This subfamily also includes tamarind Kunitz inhibitor (TKI), Enterolobium contortisiliquum trypsin inhibitor (EcTI), Acacia confusa trypsin inhibitor (AcTI), Bauhinia ungulata factor Xa inhibitor BuXI, and Phanera variegata trypsin inhibitor BvTI. TKI is a Kunitz-type dual inhibitor (TKI) of factor Xa (FXa) and trypsin. It shows prolongation of blood coagulation time. EcTI also belongs to the Kunitz family of plant inhibitors, common in plant seeds. It inhibits trypsin, chymotrypsin, plasma kallikrein, plasmin, human neutrophil elastase, and Factor XIIa in the stoichiometric ratio 1:1, but not thrombin, bovine pancreatic elastase, or Factor Xa. It is involved in the inhibition of the invasion of gastric cancer cells through alterations in integrin-dependent cell signaling pathway. AcTI inhibits trypsin and alpha-chymotrypsin stoichiometrically at the molar ratio of 1:1 and 2:1 respectively. BuXI inhibits bovine trypsin and chymotrypsin, and human plasmin, plasma kallikrein, factor XIIa, and factor Xa. BvTI inhibits bovine trypsin and chymotrypsin, and human plasmin, plasma kallikrein and factor XIIa. Members of this subfamily contain an STI-like domain with a beta-trefoil fold, which is characterized by 12 beta strands folded into three similar trefoil subdomains (alpha, beta, and gamma) associated to give an overall structure with pseudo-3-fold symmetry.


Pssm-ID: 467390  Cd Length: 170  Bit Score: 82.16  E-value: 9.90e-20
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15219441  28 VEDSVGRLLRPGQTYHIVPANPETGGGI-FSNS-EEICPLDIFQSNNPLDLGLPIKFKS--ELWFVKEMNSITIEF-EAP 102
Cdd:cd23365   2 LLDTDGDPLNNGGQYYILPALRGKGGGLeLARTgDETCPLTVVQARSETSRGLPVRISSppRIAIITTAFYLNIEFqPAP 81

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15219441 103 NwflCPKESKGWRVVYSEEFKKSLIISTGGSSNPSGFQIHRVDGgAYKIVYCTNISTT-TCMNVGIFTDISGARRLALTS 181
Cdd:cd23365  82 A---CLPKPLRWRIEQESSSEGEVKIAPDEERLFGPFQIKPYRE-DYKLVYCESSSDDdSCRDLGISIDDENNRRLVVKD 157

                       170
                ....*....|.
gi 15219441 182 DEALLVKFQKA 192
Cdd:cd23365 158 GDPLAVRFKKA 168
beta-trefoil_STI_KPI104-like cd23367
soybean trypsin inhibitor (STI)-like domain, beta-trefoil fold, found in Medicago truncatula ...
 28-191 2.30e-19

soybean trypsin inhibitor (STI)-like domain, beta-trefoil fold, found in Medicago truncatula Kunitz type trypsin inhibitor 104 (KPI104) and similar proteins; This subfamily includes Medicago truncatula KPI104, KPI106, and KPI111. They are protease inhibitors involved in the control of mycorrhiza establishment and arbuscule development during root colonization by arbuscular mycorrhizal (AM) fungi (e.g. Rhizophagus irregularis). KPI104 interacts with cysteine protease (CP). It shows a stronger affinity for serine carboxypeptidase (SCP1) than for CP. KPI111 only interacts with SCP1. Members of this subfamily contain an STI-like domain with a beta-trefoil fold, which is characterized by 12 beta strands folded into three similar trefoil subdomains (alpha, beta, and gamma) associated to give an overall structure with pseudo-3-fold symmetry.


Pssm-ID: 467392  Cd Length: 170  Bit Score: 81.24  E-value: 2.30e-19
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15219441  28 VEDSVGRLLRPGQTYHIVPANPETGGGI-FSNSEEICPLDIFQSNNPLDLGLPIKFK---SELWFVKEMNSITIEFEAPN 103
Cdd:cd23367   3 VLDTNGKPLESGVEYYIKPAITDVGGALtLVNRNNSCPLYVGQENVTPSSGLPVKFTpfvDGETVVREGRDFTITFQAST 82

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15219441 104 wfLCpKESKGWRVVYSEEFKKSLIISTGGSSNPSG-FQIHRVDGGAYKIVYCTNISTTTCM----NVGIFTdISGARRLA 178
Cdd:cd23367  83 --TC-GQSTEWRVGERDPVSGRRLITTGGENGYGNyFRIVRSNRGGYNLRWCPTEVCPNCRfrcgTVGILT-ENGKRLLA 158

                       170
                ....*....|...
gi 15219441 179 LTSDeALLVKFQK 191
Cdd:cd23367 159 LDGP-ALPVVFER 170
beta-trefoil_STI_GWIN3 cd23380
soybean trypsin inhibitor (STI)-like domain, beta-trefoil fold, found in Populus sp. ...
 28-189 1.20e-16

soybean trypsin inhibitor (STI)-like domain, beta-trefoil fold, found in Populus sp. wound-responsive protein GWIN3 and similar proteins; GWIN3 may play a role in wound response. It shows high sequence similarity with sweet potato sporamins and legume Kunitz trypsin inhibitors. It remains unclear if GWIN3 is a trypsin inhibitor, but proteinase inhibitor function would be consistent with its wound-regulated behavior. Members of this subfamily contain an STI-like domain with a beta-trefoil fold, which is characterized by 12 beta strands folded into three similar trefoil subdomains (alpha, beta, and gamma) associated to give an overall structure with pseudo-3-fold symmetry.


Pssm-ID: 467405  Cd Length: 168  Bit Score: 74.09  E-value: 1.20e-16
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15219441  28 VEDSVGRLLRPGQTYHIVPANPETGGGIFSNSEEICPLDIFQSNNPldLGLPIKF----KSELWFVKEMNSITIEFEAPn 103
Cdd:cd23380   4 VLDFNGNEVLAGAYYYIAPEDSLPFLVVAAIRPGTCWSDVILERFL--DGLPIKFspvaPSNDSVIRESTYLNIEFNAE- 80

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15219441 104 wfLCPKE--SKGWRVVYSEEFKKSlIISTGGSSNPSGFQIHRV--DGGAYKIVYCTN-ISTTTCMNVGIFTDiSGARRLA 178
Cdd:cd23380  81 --LCKVCgvTTMWKVEFNATMQQP-FVTTGGVDRLNWFKITKAeeDNRFYQLSYCPVsGIQCPCVTVGISNR-NGTERLA 156

                       170
                ....*....|.
gi 15219441 179 LTsDEALLVKF 189
Cdd:cd23380 157 LN-DEPLPFVF 166
beta-trefoil_STI_AtKTI6-like cd23369
soybean trypsin inhibitor (STI)-like domain, beta-trefoil fold, found in Arabidopsis thaliana ...
 28-189 1.17e-14

soybean trypsin inhibitor (STI)-like domain, beta-trefoil fold, found in Arabidopsis thaliana Kunitz trypsin inhibitor 6 (AtKTI6) and similar proteins; This subfamily includes AtKTI6 and AtKTI7, which exhibit Kunitz trypsin protease inhibitor activity. Members of this subfamily contain an STI-like domain with a beta-trefoil fold, which is characterized by 12 beta strands folded into three similar trefoil subdomains (alpha, beta, and gamma) associated to give an overall structure with pseudo-3-fold symmetry.


Pssm-ID: 467394  Cd Length: 170  Bit Score: 68.57  E-value: 1.17e-14
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15219441  28 VEDSVGRLLRPGQTYHIVPANPETGG----GIFSNSEEICPLDIFQSNNPLDLG-LPIKF---KSELWFVKEMNSITIEF 99
Cdd:cd23369   6 VLDTNGNPVKPGAPYYILDATNYGRGisrsQVGPDDPNPCPQTVVLGSDPLISApPPVAFvleSSSDDVVRVSTELSIRF 85

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15219441 100 EAPNwfLCPkESKGWRVVYSEEFKKSLIISTGGSSNPSGFQIHRVDGGAYKIVYCTNISTTTcmnvgIFTD-ISGARRLA 178
Cdd:cd23369  86 AEPS--HCA-ESGYWRVANSSSPKKEVVLTGSKSSNDSTFTIKKSDDGYYKFAFGSADKPTP-----LGLEnYDDIYRLV 157

                       170
                ....*....|.
gi 15219441 179 LTSDEALLVKF 189
Cdd:cd23369 158 LSPDEEFDVSF 168
beta-trefoil_STI_COTI cd23379
soybean trypsin inhibitor (STI)-like domain, beta-trefoil fold, found in Senna obtusifolia ...
 28-191 8.04e-13

soybean trypsin inhibitor (STI)-like domain, beta-trefoil fold, found in Senna obtusifolia trypsin inhibitor 1 (COTI) and similar proteins; COTI is a specific inhibitor of bovine trypsin. It also exhibits strong inhibitory effect on midgut trypsin from Pieris rapae, Helicoverpa armigera, Spodoptera exigua, and Spodoptera litura. Members of this subfamily contain an STI-like domain with a beta-trefoil fold, which is characterized by 12 beta strands folded into three similar trefoil subdomains (alpha, beta, and gamma) associated to give an overall structure with pseudo-3-fold symmetry.


Pssm-ID: 467404  Cd Length: 172  Bit Score: 63.65  E-value: 8.04e-13
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15219441  28 VEDSVGRLLRPGQTYHIVPANpeTGGGIFS-----NSEEICPLDIFQSNNplDLGLPIKFKS--ELWFVKEMNSITIEFE 100
Cdd:cd23379   2 VYDSDGDILRNGGKYFISPPN--GGGAILAaaishGSDRSCSLAVIQALS--YIGWPVTISTpfPPTFITTSFPLNISFA 77

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15219441 101 --APNwfLCPKeSKGWRVVYSEEFKKSLII-STGGSSNPSG--FQIHRVDG--GAYKIVYCTNiSTTTCMNVGIFTDISG 173
Cdd:cd23379  78 ylPPN--VCTK-SPDWVVVKSNPLGEPVMVgDFEEFDNPVSgyFYIKSYDSskGYYKLVFCYG-GDDSCGNIGVDKDSNG 153

                       170
                ....*....|....*....
gi 15219441 174 ARRLALTSD-EALLVKFQK 191
Cdd:cd23379 154 FRRLVVTDDrEPLVFKFDK 172
beta-trefoil_STI_CPI-like cd23372
soybean trypsin inhibitor (STI)-like domain, beta-trefoil fold, found in Solanum tuberosum ...
 39-192 1.43e-10

soybean trypsin inhibitor (STI)-like domain, beta-trefoil fold, found in Solanum tuberosum cysteine protease inhibitor (CPI), serine protease inhibitor (SPI), aspartic protease inhibitor (API) and similar proteins; This subfamily includes Solanum tuberosum CPI, SPI, API, and similar proteins. CPI is a Kunitz-type potato cathepsin D inhibitor. It acts as a potent inhibitor of cathepsin l (cysteine protease) but does not inhibit trypsin or chymotrypsin (serine proteases). SPI is a potent inhibitor of serine proteases (chymotrypsin and trypsin). It inhibits tightly human leukocyte elastase (HLE). It does not inhibit papain, pepsin nor cathepsin D (cysteine and aspartic proteases). API functions as the inhibitor of cathepsin D (aspartic protease). It may also inhibit trypsin and chymotrypsin (serine proteases). CPI, SPI, and API protect plants by inhibiting proteases of invading organisms. Members of this subfamily contain an STI-like domain with a beta-trefoil fold, which is characterized by 12 beta strands folded into three similar trefoil subdomains (alpha, beta, and gamma) associated to give an overall structure with pseudo-3-fold symmetry.


Pssm-ID: 467397  Cd Length: 171  Bit Score: 57.81  E-value: 1.43e-10
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15219441  39 GQTYHIVPANPET-GGGIF----SNSEEICPLDIFQSNNP-LDLGLPIKF---KSELWFVKEMNSITIEFEAPNWFLCPK 109
Cdd:cd23372   1 GESYRIISILWGAlGGDVYlgkiPNSDAPCPNGVFQYNSDvGPSGTPVRFiplSEYSGVIFENQDLNIQFSIPTSKLCVN 80

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15219441 110 ESKgWRVVYSEEFKKSLIISTGGS---SNPSGFQIHRVDGGA--YKIVYC--TNISTT---TCMNVGIFTDiSGARRLAL 179
Cdd:cd23372  81 YTV-WKVGDENASLGTMLLETGGTigqADSSWFKIVKSSLMKfgYKLLYCpsTSICPRddlFCADVGVVFQ-NGYRRLAL 158

                       170
                ....*....|...
gi 15219441 180 TSDEALLVKFQKA 192
Cdd:cd23372 159 VNDNPLDVVFQKV 171
beta-trefoil_STI_ASI cd23373
soybean trypsin inhibitor (STI)-like domain, beta-trefoil fold, found in alpha-amylase ...
 28-191 8.28e-10

soybean trypsin inhibitor (STI)-like domain, beta-trefoil fold, found in alpha-amylase/subtilisin inhibitor (ASI) and similar proteins; This subfamily includes rice ASI (RASI) and barley ASI (BASI). RASI can inhibit alpha-amylase from larvae of the red flour beetle (Tribolium castaneum) and subtilisin from Bacillus subtilis. BASI is a bifunctional protein that can simultaneously inhibit alpha-amylase isozyme (AMY2) and serine proteases of the subtilisin family. Members of this subfamily contain an STI-like domain with a beta-trefoil fold, which is characterized by 12 beta strands folded into three similar trefoil subdomains (alpha, beta, and gamma) associated to give an overall structure with pseudo-3-fold symmetry.


Pssm-ID: 467398  Cd Length: 176  Bit Score: 55.84  E-value: 8.28e-10
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15219441  28 VEDSVGRLLRPGQTYHIVPANPETGGG--IFSNSEEICPLDIFQSNNPLDLGLPIKF------KSELWFVKEMNSITIEF 99
Cdd:cd23373   5 VYDTDGHELSSDASYYVLPANRGHGGGltMAPGWLRRCPLFVSQEPDEALVGFPVRFtplgnsSSSDAAIRLSTDVRIEF 84

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15219441 100 EApnWFLCpKESKGWRVVySEEFKKSLIISTG---GSSNPSGFQIHRV-----DGGAYKIVYCtnISTTTCMNVGIFTDi 171
Cdd:cd23373  85 RA--ITTC-VQSLEWHVS-SEPSTGRRHVAAGpveGPSPPGREFVFRVerysgAEKGYKLVSC--GDKDPCRDLGLYRD- 157

                       170       180
                ....*....|....*....|
gi 15219441 172 SGARRLAlTSDEALLVKFQK 191
Cdd:cd23373 158 KKKWWLT-VSDPPHVVVFKK 176
beta-trefoil_STI_LSPI cd23371
soybean trypsin inhibitor (STI)-like domain, beta-trefoil fold, found in Carica papaya latex ...
 28-192 7.14e-09

soybean trypsin inhibitor (STI)-like domain, beta-trefoil fold, found in Carica papaya latex serine proteinase inhibitor (LSPI) and similar proteins; LSPI, also called papaya protease inhibitor (PPI), is a double-headed Kunitz-type serine protease inhibitor. A single LSPI molecule can bind two trypsin units at the same time. LSPI may serve as a defense protein as it is induced by wounding and is inactive against endogenous proteases from C. papaya. LSPI belongs to the miraculin family of taste-modifying proteins, which are active against serine proteases. Members of this subfamily contain an STI-like domain with a beta-trefoil fold, which is characterized by 12 beta strands folded into three similar trefoil subdomains (alpha, beta, and gamma) associated to give an overall structure with pseudo-3-fold symmetry.


Pssm-ID: 467396  Cd Length: 183  Bit Score: 53.40  E-value: 7.14e-09
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15219441  28 VEDSVGRLLRPGQTYHIVPANPETGGGIFS----NSEEICPLDIFQSNNPLDLGLPIKFKS----ELWFVKEMNSITIEF 99
Cdd:cd23371   3 IVDIDGKPLRYGVDYFVVSAIWGAGGGGLSlygpGNKKKCPLSVVQDPFDSDNGIPVKFSAvknvKDNIVRESTDLNVKF 82

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15219441 100 EAPnwfLCPKESKGWRVvysEEFKKSL--IISTGG-------SSNPSGFQIHRVDGGA--YKIVYCTNISTTT---CMNV 165
Cdd:cd23371  83 NIT---INCNETTVWKV---DRFPGVIgwTVTLGGvkgyhgfESTHSMFKIKRAGLVSfsYKFRFCPSYPRTRlipCNNV 156

                       170       180
                ....*....|....*....|....*..
gi 15219441 166 GIFTDISGARRLALTSDEALLVkFQKA 192
Cdd:cd23371 157 GVFSDKYRIRRLVLTDDAKEFV-FVKA 182
beta-trefoil_STI_WBA1 cd23361
soybean trypsin inhibitor (STI)-like domain, beta-trefoil fold, found in Psophocarpus ...
 28-191 7.47e-09

soybean trypsin inhibitor (STI)-like domain, beta-trefoil fold, found in Psophocarpus tetragonolobus albumin-1 and similar proteins; Albumin-1, also called WBA-1, or winged bean albumin 1, acts as a 2S seed storage protein that is homologous with Kunitz-type seed trypsin inhibitors. It contains a soybean trypsin inhibitor (STI)-like domain with a beta-trefoil fold, which is characterized by 12 beta strands folded into three similar trefoil subdomains (alpha, beta, and gamma) associated to give an overall structure with pseudo-3-fold symmetry.


Pssm-ID: 467386  Cd Length: 174  Bit Score: 53.26  E-value: 7.47e-09
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15219441  28 VEDSVGRLLRPGQTYHIVPANPETGGGIFSNSEEI--CPLDIFQSNNPLDLGlpIKFKSELWFVKEMNSITIEFEAPNWF 105
Cdd:cd23361   5 VYDAEGNKLVNRGKYTIVSFSDGAGIDVVATGNENpeDPLSIVKSTRNIMYA--TSISSEDKTPPQPRNILENMRLKINF 82

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15219441 106 LCPK-ESKGWRVVYSeeFKKSLIISTGGSSNPSGFQIHRVDGGA-----YKIVYCTNISttTCMNVGIFTDISGARRLAL 179
Cdd:cd23361  83 ATDPhKGDVWSVVDF--QPDGQQLKLAGRYPNQVKGAFTIQKGSntprtYKLLFCPVGS--PCKNIGISTDPEGKKRLVV 158

                       170
                ....*....|...
gi 15219441 180 TSD-EALLVKFQK 191
Cdd:cd23361 159 SYQsDPLVVKFHR 171
beta-trefoil_STI_API-A-like cd23381
soybean trypsin inhibitor (STI)-like domain, beta-trefoil fold, found in Sagittaria ...
 52-191 2.04e-05

soybean trypsin inhibitor (STI)-like domain, beta-trefoil fold, found in Sagittaria sagittifolia proteinase inhibitor A (API-A) and similar proteins; API-A, also called double-headed proteinase inhibitor A, inhibits an equimolar amount of trypsin and chymotrypsin simultaneously, and inhibits kallikrein weakly. It possesses two distinct reactive sites, unlike other members of their family. API-A contains an STI-like domain with a beta-trefoil fold, which is characterized by 12 beta strands folded into three similar trefoil subdomains (alpha, beta, and gamma) associated to give an overall structure with pseudo-3-fold symmetry.


Pssm-ID: 467406  Cd Length: 188  Bit Score: 43.41  E-value: 2.04e-05
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15219441  52 GGGIFSNSEEICPLDIFQSNNPLDLGLPIKF---KSELWFVKEMNSITIEFEAPNWFLCpkeSKGWRVVYSEE-----FK 123
Cdd:cd23381  27 GGLSTLRLDGTCPSYVGQAPSDVDRGLPVSFspaATSQPVVQLSSRLNISFSMSVPCIN---STVWSLGKSETnggisTQ 103

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 15219441 124 KSLIISTGGSSNPSGFQIHRV-DGGAYKIVYC------------------TNISTTTCMNVGIFTDisGARRLALTSDEA 184
Cdd:cd23381 104 NITIGDYGYLNFFSWFEIESTeETGVYKLVACsceqaigpfdvsdmgasrSGFCKIGCPEVGSFNV--GGQTLLGLSPEH 181


                ....*..
gi 15219441 185 LLVKFQK 191
Cdd:cd23381 182 FLVVFKK 188
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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