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Conserved domains on  [gi|42562784|ref|NP_176032|]
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Peptide chain release factor 2 [Arabidopsis thaliana]

Protein Classification

PCRF domain-containing protein( domain architecture ID 1750053)

peptide chain release factor (PCRF) domain-containing protein similar to Homo sapiens mitochondrial peptide chain release factor 1 (MTRF1), MTRF1-like and 28S mitochondrial ribosomal protein S35 (MRPS35)

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
PCRF super family cl40670
This domain is found in peptide chain release factors;
112-475 7.46e-147

This domain is found in peptide chain release factors;


The actual alignment was detected with superfamily member TIGR00020:

Pssm-ID: 477417 [Multi-domain]  Cd Length: 364  Bit Score: 424.19  E-value: 7.46e-147
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42562784   112 EGDWKSHAAAIAQSIQVIKRRLQWKKLLVRLKVLSAELNKSDLWDDPTHAGKISREHGSLTGKMKGVMTFERELLEHIDM 191
Cdd:TIGR00020   2 INEVNNRIEDLTSRLDTVRGSLDPEKKKARLEELEKEMEDPNFWNDQERAQAVIKERSSLEAVLDTLEELKNSLEDLSEL 81

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42562784   192 LKLAKEENDSELESETLKALIDMRRVSKEKELEALLSADNDPCSCYIEVQAGAGGTESNDWAAMVMEMYKTWAQRRKFSV 271
Cdd:TIGR00020  82 LELAVEEDDEETFNELDAELKALEKKLAELELRTMLSGEYDANNAYLTIQAGAGGTEAQDWASMLYRMYLRWAERRGFKV 161

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42562784   272 TVVDEAPGEIAGIKRATIKVNGEYAYGYAKAEVGVHRLVRISPFDSGKRRHTSFAAVAVIPILGDgSTRVEINDSDLRIE 351
Cdd:TIGR00020 162 EIIDYSEGEEAGIKSVTILIKGPYAYGYLKSEQGVHRLVRISPFDANGRRHTSFASVFVMPEVDD-DIDIEIKPEDLRID 240

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42562784   352 RFRSGGAGGQHANTTDSAVRIVHIPTGITATCQNERSQHSNKASAMAVLQSRLDQLEMARQTAMNAQHTQSLTEISWGNQ 431
Cdd:TIGR00020 241 TYRASGAGGQHVNKTDSAVRITHIPTGIVVQCQNDRSQHKNKDSAMKVLKAKLYELEMEKEQAEKDAKEGEKSEIGWGSQ 320

                         330       340       350       360
                  ....*....|....*....|....*....|....*....|....
gi 42562784   432 IRTYVLHPYRMVKDLRTNYEVSDPDSVLEGDLDGFILSFLSSSL 475
Cdd:TIGR00020 321 IRSYVLHPYSMVKDLRTGYETGNVQAVLDGDIDQFIEAYLKWKL 364
 
Name Accession Description Interval E-value
prfB TIGR00020
peptide chain release factor 2; In many but not all taxa, there is a conserved real ...
 112-475 7.46e-147

peptide chain release factor 2; In many but not all taxa, there is a conserved real translational frameshift at a TGA codon. RF-2 helps terminate translation at TGA codons and can therefore regulate its own production by readthrough when RF-2 is insufficient. There is a Pfam model called "RF-1" for the superfamily of RF-1, RF-2, mitochondrial, RF-H, etc. [Protein synthesis, Translation factors]


Pssm-ID: 272858 [Multi-domain]  Cd Length: 364  Bit Score: 424.19  E-value: 7.46e-147
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42562784   112 EGDWKSHAAAIAQSIQVIKRRLQWKKLLVRLKVLSAELNKSDLWDDPTHAGKISREHGSLTGKMKGVMTFERELLEHIDM 191
Cdd:TIGR00020   2 INEVNNRIEDLTSRLDTVRGSLDPEKKKARLEELEKEMEDPNFWNDQERAQAVIKERSSLEAVLDTLEELKNSLEDLSEL 81

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42562784   192 LKLAKEENDSELESETLKALIDMRRVSKEKELEALLSADNDPCSCYIEVQAGAGGTESNDWAAMVMEMYKTWAQRRKFSV 271
Cdd:TIGR00020  82 LELAVEEDDEETFNELDAELKALEKKLAELELRTMLSGEYDANNAYLTIQAGAGGTEAQDWASMLYRMYLRWAERRGFKV 161

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42562784   272 TVVDEAPGEIAGIKRATIKVNGEYAYGYAKAEVGVHRLVRISPFDSGKRRHTSFAAVAVIPILGDgSTRVEINDSDLRIE 351
Cdd:TIGR00020 162 EIIDYSEGEEAGIKSVTILIKGPYAYGYLKSEQGVHRLVRISPFDANGRRHTSFASVFVMPEVDD-DIDIEIKPEDLRID 240

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42562784   352 RFRSGGAGGQHANTTDSAVRIVHIPTGITATCQNERSQHSNKASAMAVLQSRLDQLEMARQTAMNAQHTQSLTEISWGNQ 431
Cdd:TIGR00020 241 TYRASGAGGQHVNKTDSAVRITHIPTGIVVQCQNDRSQHKNKDSAMKVLKAKLYELEMEKEQAEKDAKEGEKSEIGWGSQ 320

                         330       340       350       360
                  ....*....|....*....|....*....|....*....|....
gi 42562784   432 IRTYVLHPYRMVKDLRTNYEVSDPDSVLEGDLDGFILSFLSSSL 475
Cdd:TIGR00020 321 IRSYVLHPYSMVKDLRTGYETGNVQAVLDGDIDQFIEAYLKWKL 364
PRK07342 PRK07342
peptide chain release factor 2; Provisional
 154-481 9.90e-126

peptide chain release factor 2; Provisional


Pssm-ID: 235997 [Multi-domain]  Cd Length: 339  Bit Score: 369.59  E-value: 9.90e-126
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42562784  154 LWDDPTHAGKISREHGSLTGKMKGVMTFERELLEHIDMLKLAKEENDSELESETLKALIDMRRVSKEKELEALLSADNDP 233
Cdd:PRK07342   8 LWNDAQEAQKLMRERQQLDDSINGINHLEQTLNDNIELIAMGEEEGDKSIVEDAEKTIRDLKDEIDRRQIDALLSGEADA 87

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42562784  234 CSCYIEVQAGAGGTESNDWAAMVMEMYKTWAQRRKFSVTVVDEAPGEIAGIKRATIKVNGEYAYGYAKAEVGVHRLVRIS 313
Cdd:PRK07342  88 NDTYLEVHAGAGGTESQDWASMLLRMYTRWAERQGRKVEVLEVHDGEEAGIKSATILVKGHNAYGWLKTESGVHRLVRIS 167

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42562784  314 PFDSGKRRHTSFAAVAVIPILgDGSTRVEINDSDLRIERFRSGGAGGQHANTTDSAVRIVHIPTGITATCQNERSQHSNK 393
Cdd:PRK07342 168 PYDSNARRHTSFASIWVYPVI-DDNIEVDVNESDVRIDTYRSSGAGGQHVNTTDSAVRITHIPTGIVVQCQQERSQHKNR 246

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42562784  394 ASAMAVLQSRLDQLEM-ARQTAMNAQHTqSLTEISWGNQIRTYVLHPYRMVKDLRTNYEVSDPDSVLEGDLDGFILSFLS 472
Cdd:PRK07342 247 AKAWSMLRARLYEEELkKREEATNAAAA-SKTDIGWGHQIRSYVLQPYQLVKDLRTGVESTNPQDVLDGDLNEFMEAALA 325


                 ....*....
gi 42562784  473 SSLDKDDPE 481
Cdd:PRK07342 326 HRISGGADM 334
PrfA COG0216
Protein chain release factor RF1 [Translation, ribosomal structure and biogenesis]; Protein ...
 134-467 2.93e-84

Protein chain release factor RF1 [Translation, ribosomal structure and biogenesis]; Protein chain release factor RF1 is part of the Pathway/BioSystem: Translation factors


Pssm-ID: 439986 [Multi-domain]  Cd Length: 356  Bit Score: 263.78  E-value: 2.93e-84
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42562784 134 QWKKLLVRLKVLSAELNKSDLWDDPTHAGKISREHGSLTgkmKGVMTFER--ELLEHIDMLK-LAKEENDSEL------E 204
Cdd:COG0216   5 KLEALEERYEELEALLSDPEVISDQKRFRKLSKEYAELE---PIVEAYREykKLLEDIEEAKeLLEEESDPEMremakeE 81

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42562784 205 SETLKAlidmRRVSKEKELE-ALLSAD-NDPCSCYIEVQAGAGGTESNDWAAMVMEMYKTWAQRRKFSVTVVDEAPGEIA 282
Cdd:COG0216  82 LEELEA----RLEELEEELKiLLLPKDpNDDKNVILEIRAGTGGDEAALFAGDLFRMYSRYAERKGWKVEILSASEGELG 157

                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42562784 283 GIKRATIKVNGEYAYGYAKAEVGVHRLVRISPFDSGKRRHTSFAAVAVIPILGDgsTRVEINDSDLRIERFRSGGAGGQH 362
Cdd:COG0216 158 GYKEVIFRISGEGVYSRLKFESGVHRVQRVPETESQGRIHTSAATVAVLPEAEE--VDVEINPKDLRIDTFRSSGAGGQH 235

                       250       260       270       280       290       300       310       320
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42562784 363 ANTTDSAVRIVHIPTGITATCQNERSQHSNKASAMAVLQSRLDQLEMARQTAMNAQHTQSLteISWGN---QIRTYvlhP 439
Cdd:COG0216 236 VNTTDSAVRITHLPTGIVVECQDERSQHKNKAKAMKVLRARLYEAEQQKQQAEIAAERKSQ--VGSGDrseRIRTY---N 310

                       330       340       350
                ....*....|....*....|....*....|.
gi 42562784 440 Y---RmVKDLRTNYEVSDPDSVLEGDLDGFI 467
Cdd:COG0216 311 FpqgR-VTDHRINLTLYKLDEVMEGDLDELI 340
PCRF pfam03462
PCRF domain; This domain is found in peptide chain release factors.
 138-328 6.83e-68

PCRF domain; This domain is found in peptide chain release factors.


Pssm-ID: 460929 [Multi-domain]  Cd Length: 192  Bit Score: 215.71  E-value: 6.83e-68
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42562784   138 LLVRLKVLSAELNKSDLWDDPTHAGKISREHGSLTGKMKGVMTFE---------RELLEHIDMLKLAKEENDsELESEtL 208
Cdd:pfam03462   1 LEERYEELEALLSDPDVWDDQKRAQKLSKEYSELEPIVEAYREYKqaledleeaKELLEDPELAELAEEELE-ELEKR-L 78

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42562784   209 KALidmrrvskEKELEALLSAD--NDPCSCYIEVQAGAGGTESNDWAAMVMEMYKTWAQRRKFSVTVVDEAPGEIAGIKR 286
Cdd:pfam03462  79 EEL--------EEELKLLLLPKdpNDDKNAILEIRAGAGGDEAALFAGDLFRMYTRYAERKGWKVEVLDYSETEAGGIKE 150

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|..
gi 42562784   287 ATIKVNGEYAYGYAKAEVGVHRLVRISPFDSGKRRHTSFAAV 328
Cdd:pfam03462 151 ATFEISGEGAYGRLKFESGVHRVQRVPPTESQGRIHTSFATV 192
PCRF smart00937
This domain is found in peptide chain release factors;
184-303 3.79e-39

This domain is found in peptide chain release factors;


Pssm-ID: 214923 [Multi-domain]  Cd Length: 116  Bit Score: 137.59  E-value: 3.79e-39
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42562784    184 ELLEHI---DMLKLAKEENDsELESETLKALidmrrvsKEKELEALLSAD-NDPCSCYIEVQAGAGGTESNDWAAMVMEM 259
Cdd:smart00937   1 ELLEEEeddEMRELAEEELE-ELEAELEEEL-------EELLKLLLLPKDpNDDKNAILEIRAGAGGDEAALFAGDLLRM 72

                           90       100       110       120
                   ....*....|....*....|....*....|....*....|....
gi 42562784    260 YKTWAQRRKFSVTVVDEAPGEIAGIKRATIKVNGEYAYGYAKAE 303
Cdd:smart00937  73 YLRYAERKGWKVEVLDESEGELGGIKEATLKISGEGAYGKLKFE 116
 
Name Accession Description Interval E-value
prfB TIGR00020
peptide chain release factor 2; In many but not all taxa, there is a conserved real ...
 112-475 7.46e-147

peptide chain release factor 2; In many but not all taxa, there is a conserved real translational frameshift at a TGA codon. RF-2 helps terminate translation at TGA codons and can therefore regulate its own production by readthrough when RF-2 is insufficient. There is a Pfam model called "RF-1" for the superfamily of RF-1, RF-2, mitochondrial, RF-H, etc. [Protein synthesis, Translation factors]


Pssm-ID: 272858 [Multi-domain]  Cd Length: 364  Bit Score: 424.19  E-value: 7.46e-147
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42562784   112 EGDWKSHAAAIAQSIQVIKRRLQWKKLLVRLKVLSAELNKSDLWDDPTHAGKISREHGSLTGKMKGVMTFERELLEHIDM 191
Cdd:TIGR00020   2 INEVNNRIEDLTSRLDTVRGSLDPEKKKARLEELEKEMEDPNFWNDQERAQAVIKERSSLEAVLDTLEELKNSLEDLSEL 81

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42562784   192 LKLAKEENDSELESETLKALIDMRRVSKEKELEALLSADNDPCSCYIEVQAGAGGTESNDWAAMVMEMYKTWAQRRKFSV 271
Cdd:TIGR00020  82 LELAVEEDDEETFNELDAELKALEKKLAELELRTMLSGEYDANNAYLTIQAGAGGTEAQDWASMLYRMYLRWAERRGFKV 161

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42562784   272 TVVDEAPGEIAGIKRATIKVNGEYAYGYAKAEVGVHRLVRISPFDSGKRRHTSFAAVAVIPILGDgSTRVEINDSDLRIE 351
Cdd:TIGR00020 162 EIIDYSEGEEAGIKSVTILIKGPYAYGYLKSEQGVHRLVRISPFDANGRRHTSFASVFVMPEVDD-DIDIEIKPEDLRID 240

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42562784   352 RFRSGGAGGQHANTTDSAVRIVHIPTGITATCQNERSQHSNKASAMAVLQSRLDQLEMARQTAMNAQHTQSLTEISWGNQ 431
Cdd:TIGR00020 241 TYRASGAGGQHVNKTDSAVRITHIPTGIVVQCQNDRSQHKNKDSAMKVLKAKLYELEMEKEQAEKDAKEGEKSEIGWGSQ 320

                         330       340       350       360
                  ....*....|....*....|....*....|....*....|....
gi 42562784   432 IRTYVLHPYRMVKDLRTNYEVSDPDSVLEGDLDGFILSFLSSSL 475
Cdd:TIGR00020 321 IRSYVLHPYSMVKDLRTGYETGNVQAVLDGDIDQFIEAYLKWKL 364
PRK07342 PRK07342
peptide chain release factor 2; Provisional
 154-481 9.90e-126

peptide chain release factor 2; Provisional


Pssm-ID: 235997 [Multi-domain]  Cd Length: 339  Bit Score: 369.59  E-value: 9.90e-126
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42562784  154 LWDDPTHAGKISREHGSLTGKMKGVMTFERELLEHIDMLKLAKEENDSELESETLKALIDMRRVSKEKELEALLSADNDP 233
Cdd:PRK07342   8 LWNDAQEAQKLMRERQQLDDSINGINHLEQTLNDNIELIAMGEEEGDKSIVEDAEKTIRDLKDEIDRRQIDALLSGEADA 87

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42562784  234 CSCYIEVQAGAGGTESNDWAAMVMEMYKTWAQRRKFSVTVVDEAPGEIAGIKRATIKVNGEYAYGYAKAEVGVHRLVRIS 313
Cdd:PRK07342  88 NDTYLEVHAGAGGTESQDWASMLLRMYTRWAERQGRKVEVLEVHDGEEAGIKSATILVKGHNAYGWLKTESGVHRLVRIS 167

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42562784  314 PFDSGKRRHTSFAAVAVIPILgDGSTRVEINDSDLRIERFRSGGAGGQHANTTDSAVRIVHIPTGITATCQNERSQHSNK 393
Cdd:PRK07342 168 PYDSNARRHTSFASIWVYPVI-DDNIEVDVNESDVRIDTYRSSGAGGQHVNTTDSAVRITHIPTGIVVQCQQERSQHKNR 246

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42562784  394 ASAMAVLQSRLDQLEM-ARQTAMNAQHTqSLTEISWGNQIRTYVLHPYRMVKDLRTNYEVSDPDSVLEGDLDGFILSFLS 472
Cdd:PRK07342 247 AKAWSMLRARLYEEELkKREEATNAAAA-SKTDIGWGHQIRSYVLQPYQLVKDLRTGVESTNPQDVLDGDLNEFMEAALA 325


                 ....*....
gi 42562784  473 SSLDKDDPE 481
Cdd:PRK07342 326 HRISGGADM 334
PRK05589 PRK05589
peptide chain release factor 2; Provisional
 149-473 7.91e-110

peptide chain release factor 2; Provisional


Pssm-ID: 235520 [Multi-domain]  Cd Length: 325  Bit Score: 328.30  E-value: 7.91e-110
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42562784  149 LNKSDLWDDPTHAGKISREHGSLTGKMKGVMTFERELlEHIDMLKLAKEENDSELESETLKALIDMRRVSKEKELEALLS 228
Cdd:PRK05589   1 MQEPNFWNDIKEAQEITSEEKYLKDKLDKYNHLRNRI-EDIEVLCEMMSEEDDEMKKEIISEVKNIKEEIDRFKIETLLS 79

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42562784  229 ADNDPCSCYIEVQAGAGGTESNDWAAMVMEMYKTWAQRRKFSVTVVDEAPGEIAGIKRATIKVNGEYAYGYAKAEVGVHR 308
Cdd:PRK05589  80 GEYDRNNAILTLHSGVGGTDAQDWTEMLLRMYTRWAEKKGYKVEIIDLLEGDEAGIKSVTLKITGEFAYGYLKAEKGIHR 159

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42562784  309 LVRISPFDSGKRRHTSFAAVAVIPILGDgSTRVEINDSDLRIERFRSGGAGGQHANTTDSAVRIVHIPTGITATCQNERS 388
Cdd:PRK05589 160 LVRISPFNANGKRQTSFASVEVLPELTD-DQDIEIRSEDLKIDTYRAGGAGGQHVNKTESAVRITHIPTGIVVQCQNERS 238

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42562784  389 QHSNKASAMAVLQSRLDQLEMARQTAMNAQHTQSLTEISWGNQIRTYVLHPYRMVKDLRTNYEVSDPDSVLEGDLDGFIL 468
Cdd:PRK05589 239 QHSNKETAMKMLKSKLVELKERAHKEKIEDLTGELKDMGWGSQIRSYVFHPYNLVKDHRTGVETSNVDSVMDGDIDNFIT 318


                 ....*
gi 42562784  469 SFLSS 473
Cdd:PRK05589 319 QYLKG 323
PRK08787 PRK08787
peptide chain release factor 2; Provisional
 170-475 8.79e-96

peptide chain release factor 2; Provisional


Pssm-ID: 136970 [Multi-domain]  Cd Length: 313  Bit Score: 291.95  E-value: 8.79e-96
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42562784  170 SLTGKMKGVMTFERELLEHIDMLKLAKEENDSELESETLkALIDMRRVSKEK-ELEALLSADNDPCSCYIEVQAGAGGTE 248
Cdd:PRK08787   1 MLEKTVIGIADVLSGLADAGELLDLAESEQDEDTALAVI-ADLDKYQAHVEKlEFQRMFSGQMDGANAFVDIQAGAGGTE 79

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42562784  249 SNDWAAMVMEMYKTWAQRRKFSVTVVDEAPGEIAGIKRATIKVNGEYAYGYAKAEVGVHRLVRISPFDSGKRRHTSFAAV 328
Cdd:PRK08787  80 AQDWAEILLRMYLRWAESRGWKTELMEVSGGEVAGIKSATVRIEGEYAYGWLKTEIGVHRLVRKSPFDSDNRRHTSFTSV 159

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42562784  329 AVIPILgDGSTRVEINDSDLRIERFRSGGAGGQHANTTDSAVRIVHIPTGITATCQNERSQHSNKASAMAVLQSRLDQLE 408
Cdd:PRK08787 160 FVSPEV-DDNIEIDINPADLRTDVYRSSGAGGQHVNKTESAVRITHIPTNTVVACQTGRSQHQNRDNAMKMLAAKLYELE 238

                        250       260       270       280       290       300
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 42562784  409 MARQTAMNAQHTQSLTEISWGNQIRTYVLHPYRmVKDLRTNYEVSDPDSVLEGDLDGFILSFLSSSL 475
Cdd:PRK08787 239 VQKRNAEKDALEATKSDIGWGSQIRNYVLDQSR-IKDLRTGIERSDTQKVLDGDLDEFVEASLKAGL 304
PRK06746 PRK06746
peptide chain release factor 2; Provisional
 154-475 2.35e-91

peptide chain release factor 2; Provisional


Pssm-ID: 75726 [Multi-domain]  Cd Length: 326  Bit Score: 281.24  E-value: 2.35e-91
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42562784  154 LWDDPTHAGKISREHGSL---TGKMKGV-MTFERELLEHidmlKLAKEENDSELESETLKALIDMRRVSKEKELEALLSA 229
Cdd:PRK06746   6 FWDDQQGAQAVINEANALkdmVGKFRQLdETFENLEITH----ELLKEEYDEDLHEELESEVKGLIQEMNEYELQLLLSD 81

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42562784  230 DNDPCSCYIEVQAGAGGTESNDWAAMVMEMYKTWAQRRKFSVTVVDEAPGEIAGIKRATIKVNGEYAYGYAKAEVGVHRL 309
Cdd:PRK06746  82 PYDKNNAILELHPGAGGTESQDWGSMLLRMYTRWAEKRGFKVETVDYLPGDEAGIKSVTLLIKGHNAYGYLKAEKGVHRL 161

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42562784  310 VRISPFDSGKRRHTSFAAVAVIPILGDgSTRVEINDSDLRIERFRSGGAGGQHANTTDSAVRIVHIPTGITATCQNERSQ 389
Cdd:PRK06746 162 VRISPFDSSGRRHTSFVSCEVVPEFND-EVEIEVRTEDLKIDTYRASGAGGQHVNTTDSAVRITHTPTNTVVTCQSERSQ 240

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42562784  390 HSNKASAMAVLQSRLDQLEMARQTAMNAQHTQSLTEISWGNQIRTYVLHPYRMVKDLRTNYEVSDPDSVLEGDLDGFILS 469
Cdd:PRK06746 241 IKNREHAMKMLKAKLYQKKLEEQQAELDEIRGEQKEIGWGSQIRSYVFHPYSLVKDHRTNTEVGNVQAVMDGEIDPFIDA 320


                 ....*.
gi 42562784  470 FLSSSL 475
Cdd:PRK06746 321 YLRSRI 326
prfA PRK00591
peptide chain release factor 1; Validated
 129-467 6.02e-85

peptide chain release factor 1; Validated


Pssm-ID: 234801 [Multi-domain]  Cd Length: 359  Bit Score: 265.40  E-value: 6.02e-85
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42562784  129 IKRRLQwkKLLVRLKVLSAELNKSDLWDDPTHAGKISREHGSLTgkmKGVMTFE--RELLEHIDMLK-LAKEENDSEL-- 203
Cdd:PRK00591   4 MLDKLE--ALEERYEELEALLSDPEVISDQKRFRKLSKEYAELE---PIVEAYReyKQAQEDLEEAKeMLEEESDPEMre 78

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42562784  204 ----ESETLKALIDmrrvSKEKELE-ALLSAD-NDPCSCYIEVQAGAGGTESNDWAAMVMEMYKTWAQRRKFSVTVVDEA 277
Cdd:PRK00591  79 makeELKELEERLE----ELEEELKiLLLPKDpNDDKNVILEIRAGTGGDEAALFAGDLFRMYSRYAERQGWKVEILSAS 154

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42562784  278 PGEIAGIKRATIKVNGEYAYGYAKAEVGVHRLVRISPFDSGKRRHTSFAAVAVIPILGDgsTRVEINDSDLRIERFRSGG 357
Cdd:PRK00591 155 EGELGGYKEVIAEISGDGVYSKLKFESGVHRVQRVPATESQGRIHTSAATVAVLPEAEE--VEVEINPKDLRIDTFRSSG 232

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42562784  358 AGGQHANTTDSAVRIVHIPTGITATCQNERSQHSNKASAMAVLQSRLDQLEMARQTAMNAQHTQSLteISWGN---QIRT 434
Cdd:PRK00591 233 AGGQHVNTTDSAVRITHLPTGIVVECQDERSQHKNKAKAMKVLRARLYDAERQKAQAEEAATRKSQ--VGSGDrseRIRT 310

                        330       340       350
                 ....*....|....*....|....*....|...
gi 42562784  435 YVlHPYRMVKDLRTNYEVSDPDSVLEGDLDGFI 467
Cdd:PRK00591 311 YN-FPQGRVTDHRINLTLYKLDEVMEGDLDELI 342
PrfA COG0216
Protein chain release factor RF1 [Translation, ribosomal structure and biogenesis]; Protein ...
 134-467 2.93e-84

Protein chain release factor RF1 [Translation, ribosomal structure and biogenesis]; Protein chain release factor RF1 is part of the Pathway/BioSystem: Translation factors


Pssm-ID: 439986 [Multi-domain]  Cd Length: 356  Bit Score: 263.78  E-value: 2.93e-84
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42562784 134 QWKKLLVRLKVLSAELNKSDLWDDPTHAGKISREHGSLTgkmKGVMTFER--ELLEHIDMLK-LAKEENDSEL------E 204
Cdd:COG0216   5 KLEALEERYEELEALLSDPEVISDQKRFRKLSKEYAELE---PIVEAYREykKLLEDIEEAKeLLEEESDPEMremakeE 81

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42562784 205 SETLKAlidmRRVSKEKELE-ALLSAD-NDPCSCYIEVQAGAGGTESNDWAAMVMEMYKTWAQRRKFSVTVVDEAPGEIA 282
Cdd:COG0216  82 LEELEA----RLEELEEELKiLLLPKDpNDDKNVILEIRAGTGGDEAALFAGDLFRMYSRYAERKGWKVEILSASEGELG 157

                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42562784 283 GIKRATIKVNGEYAYGYAKAEVGVHRLVRISPFDSGKRRHTSFAAVAVIPILGDgsTRVEINDSDLRIERFRSGGAGGQH 362
Cdd:COG0216 158 GYKEVIFRISGEGVYSRLKFESGVHRVQRVPETESQGRIHTSAATVAVLPEAEE--VDVEINPKDLRIDTFRSSGAGGQH 235

                       250       260       270       280       290       300       310       320
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42562784 363 ANTTDSAVRIVHIPTGITATCQNERSQHSNKASAMAVLQSRLDQLEMARQTAMNAQHTQSLteISWGN---QIRTYvlhP 439
Cdd:COG0216 236 VNTTDSAVRITHLPTGIVVECQDERSQHKNKAKAMKVLRARLYEAEQQKQQAEIAAERKSQ--VGSGDrseRIRTY---N 310

                       330       340       350
                ....*....|....*....|....*....|.
gi 42562784 440 Y---RmVKDLRTNYEVSDPDSVLEGDLDGFI 467
Cdd:COG0216 311 FpqgR-VTDHRINLTLYKLDEVMEGDLDELI 340
PCRF pfam03462
PCRF domain; This domain is found in peptide chain release factors.
 138-328 6.83e-68

PCRF domain; This domain is found in peptide chain release factors.


Pssm-ID: 460929 [Multi-domain]  Cd Length: 192  Bit Score: 215.71  E-value: 6.83e-68
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42562784   138 LLVRLKVLSAELNKSDLWDDPTHAGKISREHGSLTGKMKGVMTFE---------RELLEHIDMLKLAKEENDsELESEtL 208
Cdd:pfam03462   1 LEERYEELEALLSDPDVWDDQKRAQKLSKEYSELEPIVEAYREYKqaledleeaKELLEDPELAELAEEELE-ELEKR-L 78

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42562784   209 KALidmrrvskEKELEALLSAD--NDPCSCYIEVQAGAGGTESNDWAAMVMEMYKTWAQRRKFSVTVVDEAPGEIAGIKR 286
Cdd:pfam03462  79 EEL--------EEELKLLLLPKdpNDDKNAILEIRAGAGGDEAALFAGDLFRMYTRYAERKGWKVEVLDYSETEAGGIKE 150

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|..
gi 42562784   287 ATIKVNGEYAYGYAKAEVGVHRLVRISPFDSGKRRHTSFAAV 328
Cdd:pfam03462 151 ATFEISGEGAYGRLKFESGVHRVQRVPPTESQGRIHTSFATV 192
RF-1 pfam00472
RF-1 domain; This domain is found in peptide chain release factors such as RF-1 and RF-2, and ...
 341-447 1.11e-53

RF-1 domain; This domain is found in peptide chain release factors such as RF-1 and RF-2, and a number of smaller proteins of unknown function. This domain contains the peptidyl-tRNA hydrolase activity. The domain contains a highly conserved motif GGQ, where the glutamine is thought to coordinate the water that mediates the hydrolysis.


Pssm-ID: 459823 [Multi-domain]  Cd Length: 111  Bit Score: 175.69  E-value: 1.11e-53
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42562784   341 VEINDSDLRIERFRSGGAGGQHANTTDSAVRIVHIPTGITATCQNERSQHSNKASAMAVLQSRLDQLEMARQTAMNAQHT 420
Cdd:pfam00472   5 IEIDPKDLRIDTFRSSGAGGQHVNKTDSAVRLTHIPTGIVVKCQDERSQHQNREKALKRLRARLYELEEEKEEEKKRRIK 84

                          90       100
                  ....*....|....*....|....*..
gi 42562784   421 QSLTEISWGNQIRTYVLHPYRMVKDLR 447
Cdd:pfam00472  85 SQVGSGDRSEQIRTYNFKPQSRVKDHR 111
PCRF smart00937
This domain is found in peptide chain release factors;
184-303 3.79e-39

This domain is found in peptide chain release factors;


Pssm-ID: 214923 [Multi-domain]  Cd Length: 116  Bit Score: 137.59  E-value: 3.79e-39
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42562784    184 ELLEHI---DMLKLAKEENDsELESETLKALidmrrvsKEKELEALLSAD-NDPCSCYIEVQAGAGGTESNDWAAMVMEM 259
Cdd:smart00937   1 ELLEEEeddEMRELAEEELE-ELEAELEEEL-------EELLKLLLLPKDpNDDKNAILEIRAGAGGDEAALFAGDLLRM 72

                           90       100       110       120
                   ....*....|....*....|....*....|....*....|....
gi 42562784    260 YKTWAQRRKFSVTVVDEAPGEIAGIKRATIKVNGEYAYGYAKAE 303
Cdd:smart00937  73 YLRYAERKGWKVEVLDESEGELGGIKEATLKISGEGAYGKLKFE 116
PrfB COG1186
Protein chain release factor PrfB [Translation, ribosomal structure and biogenesis]; Protein ...
 338-456 4.31e-38

Protein chain release factor PrfB [Translation, ribosomal structure and biogenesis]; Protein chain release factor PrfB is part of the Pathway/BioSystem: Translation factors


Pssm-ID: 440799 [Multi-domain]  Cd Length: 140  Bit Score: 135.64  E-value: 4.31e-38
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42562784 338 STRVEINDSDLRIERFRSGGAGGQHANTTDSAVRIVH------------IPTGITATCQNERSQHSNKASAMAVLQSRLD 405
Cdd:COG1186  18 SPEIEIPESDLRIDTYRASGAGGQNVNKVSSAVRLTHdiassslpdsrlTPGGIVVKAQEERSQHQNREDALKRLKALLY 97

                        90       100       110       120       130
                ....*....|....*....|....*....|....*....|....*....|..
gi 42562784 406 QLEMARQTAMNAQHTQ-SLTEISWGNQIRTyvlhpyrMVKDLRTNyeVSDPD 456
Cdd:COG1186  98 EAELEPKERRATKPTRgSKKRRLWGKQIRS-------MVKDLRGN--VDDGD 140
release_prfH TIGR03072
putative peptide chain release factor H; Members of this protein family are bacterial proteins ...
 237-435 7.08e-37

putative peptide chain release factor H; Members of this protein family are bacterial proteins homologous to peptide chain release factors 1 (RF-1, product of the prfA gene), and 2 (RF-2, product of the prfB gene). The member from Escherichia coli K-12, designated prfH, appears to be a pseudogene. This class I release factor is always found as the downstream gene of a two-gene operon. [Protein synthesis, Translation factors]


Pssm-ID: 213768  Cd Length: 200  Bit Score: 134.51  E-value: 7.08e-37
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42562784   237 YIEVQAGAGGTESNDWAAMVMEMYKTWAQRRKFSVTVVDEAPGEIAG-IKRATIKVNGEYAYGYAKAEVGVHRLVRISPF 315
Cdd:TIGR03072   2 LLQLSSAQGPAECCLAVAKALERLTREAAARGVRVEVLEQEPGEVPGtLRSALVSLDGEAAAALADRWEGTLLWICPSPY 81

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42562784   316 DSGKRRHTSFAAVAVIpilgdgSTRVEINDSDLRIERFRSGGAGGQHANTTDSAVRIVHIPTGITATCQNERSQHSNKAS 395
Cdd:TIGR03072  82 RPHHRRKNWFIGVQRF------SASEEATEDEIRFETLRSSGPGGQHVNKTESAVRATHLASGISVKVQSERSQHANKRL 155

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|
gi 42562784   396 AMAVLQSRLDQLEMARQTAMNAQHTQSLTEISWGNQIRTY 435
Cdd:TIGR03072 156 ATLLLAVRLADLQQEQAAALRAERRTAHHQIERGNPVRVF 195
PRK09256 PRK09256
aminoacyl-tRNA hydrolase;
338-402 1.11e-05

aminoacyl-tRNA hydrolase;


Pssm-ID: 181730 [Multi-domain]  Cd Length: 138  Bit Score: 45.14  E-value: 1.11e-05
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 42562784  338 STRVEINDSDLRiERF-RSGGAGGQHANTTDSAV-------------------------RIVhiPTG-ITATCQNERSQH 390
Cdd:PRK09256   5 TRRLVIPENELE-WRFiRASGPGGQNVNKVSTAVelrfdiaasslpefykerllalaghRIT--KDGvIVIKAQEFRSQE 81

                         90
                 ....*....|..
gi 42562784  391 SNKASAMAVLQS 402
Cdd:PRK09256  82 RNREDALERLVA 93
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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