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Conserved domains on  [gi|254588054|ref|NP_084484|]
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probable tRNA (uracil-O(2)-)-methyltransferase [Mus musculus]

Protein Classification

class I SAM-dependent methyltransferase( domain architecture ID 4222)

class I SAM-dependent methyltransferase that catalyzes the methylation of one or more specific substrates using S-adenosyl-L-methionine (SAM or AdoMet) as the methyl donor; similar to tRNA (uracil-O(2)-)-methyltransferase that catalyzes the formation of O(2)-methyluracil at position 44 (Um44) in tRNA(Ser)

CATH:  2.20.25.110
EC:  2.1.1.-
Gene Ontology:  GO:0008168|GO:1904047
PubMed:  12504684|12826405
SCOP:  3000118

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
AdoMet_MTase super family cl06678
Predicted AdoMet-dependent methyltransferase; Proteins in this family have been predicted to ...
 252-363 4.77e-32

Predicted AdoMet-dependent methyltransferase; Proteins in this family have been predicted to function as AdoMet-dependent methyltransferases.


The actual alignment was detected with superfamily member pfam07757:

Pssm-ID: 462255  Cd Length: 115  Bit Score: 120.13  E-value: 4.77e-32
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 254588054  252 LVSILRYSRMYQELKEKY-RDMVKVWP---EVTDPEKFVYEDVAIATYLLILWEEERAEKGVTtkqSFVDLGCGNGLLVH 327
Cdd:pfam07757   1 LVEREKFNDLYKELKDKHaKQLLDHWKtaqEKTDPGKFIFEDLAIAAFLIELWKQTQSENKEF---AFADLGCGNGLLVH 77

                          90       100       110
                  ....*....|....*....|....*....|....*...
gi 254588054  328 ILSNEGHPGRGIDIRRRKIWDMYGPQTQ--LEEGSITP 363
Cdd:pfam07757  78 LLLAEGYKGYGFDARKRKLWELYPLDTQrkLIEKALEP 115
 
Name Accession Description Interval E-value
AdoMet_MTase pfam07757
Predicted AdoMet-dependent methyltransferase; Proteins in this family have been predicted to ...
 252-363 4.77e-32

Predicted AdoMet-dependent methyltransferase; Proteins in this family have been predicted to function as AdoMet-dependent methyltransferases.


Pssm-ID: 462255  Cd Length: 115  Bit Score: 120.13  E-value: 4.77e-32
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 254588054  252 LVSILRYSRMYQELKEKY-RDMVKVWP---EVTDPEKFVYEDVAIATYLLILWEEERAEKGVTtkqSFVDLGCGNGLLVH 327
Cdd:pfam07757   1 LVEREKFNDLYKELKDKHaKQLLDHWKtaqEKTDPGKFIFEDLAIAAFLIELWKQTQSENKEF---AFADLGCGNGLLVH 77

                          90       100       110
                  ....*....|....*....|....*....|....*...
gi 254588054  328 ILSNEGHPGRGIDIRRRKIWDMYGPQTQ--LEEGSITP 363
Cdd:pfam07757  78 LLLAEGYKGYGFDARKRKLWELYPLDTQrkLIEKALEP 115
 
Name Accession Description Interval E-value
AdoMet_MTase pfam07757
Predicted AdoMet-dependent methyltransferase; Proteins in this family have been predicted to ...
 252-363 4.77e-32

Predicted AdoMet-dependent methyltransferase; Proteins in this family have been predicted to function as AdoMet-dependent methyltransferases.


Pssm-ID: 462255  Cd Length: 115  Bit Score: 120.13  E-value: 4.77e-32
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 254588054  252 LVSILRYSRMYQELKEKY-RDMVKVWP---EVTDPEKFVYEDVAIATYLLILWEEERAEKGVTtkqSFVDLGCGNGLLVH 327
Cdd:pfam07757   1 LVEREKFNDLYKELKDKHaKQLLDHWKtaqEKTDPGKFIFEDLAIAAFLIELWKQTQSENKEF---AFADLGCGNGLLVH 77

                          90       100       110
                  ....*....|....*....|....*....|....*...
gi 254588054  328 ILSNEGHPGRGIDIRRRKIWDMYGPQTQ--LEEGSITP 363
Cdd:pfam07757  78 LLLAEGYKGYGFDARKRKLWELYPLDTQrkLIEKALEP 115
Methyltransf_32 pfam13679
Methyltransferase domain; This family appears to be a methyltransferase domain.
 301-406 1.78e-03

Methyltransferase domain; This family appears to be a methyltransferase domain.


Pssm-ID: 379330 [Multi-domain]  Cd Length: 138  Bit Score: 39.09  E-value: 1.78e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 254588054  301 EEERAEKGVTTkqsFVDLGCGNGLLVHILSNEGHPGR--GIDIRR------RKIWDMYG--PQTQLEEGSITPSD-ETLF 369
Cdd:pfam13679  18 KELLDENGPIT---IVDHGAGKGYLGFILYYLKYGVRvyGIDTRAelvekaNALAQKLGfnKRMSFLEGTIAGSTpVELP 94

                          90       100       110
                  ....*....|....*....|....*....|....*....
gi 254588054  370 PGVDWLIGNHS-DELTPWIPVIAARSSytCRFFVL-PCC 406
Cdd:pfam13679  95 DRVDVVTALHAcDTATDDALRFALAKQ--ARAIVLvPCC 131
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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