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Conserved domains on  [gi|21312189|ref|NP_081197|]
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iron-sulfur cluster assembly 1 homolog, mitochondrial precursor [Mus musculus]

Protein Classification

HesB/IscA family protein( domain architecture ID 10001059)

HesB/IscA family protein is a scaffold protein upon which 2Fe-2S clusters are assembled and subsequently transferred to acceptor proteins; similar to iron-sulfur assembly protein IscA that is involved in the maturation of mitochondrial 4Fe-4S proteins functioning late in the iron-sulfur cluster assembly pathway

Gene Ontology:  GO:0016226|GO:0051536
PubMed:  32108236

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
IscA COG0316
Fe-S cluster assembly iron-binding protein IscA [Posttranslational modification, protein ...
 24-129 3.16e-47

Fe-S cluster assembly iron-binding protein IscA [Posttranslational modification, protein turnover, chaperones];


:

Pssm-ID: 440085 [Multi-domain]  Cd Length: 107  Bit Score: 147.61  E-value: 3.16e-47
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 21312189  24 ALTLTPSAVNKIKQLL-KDKPEHVGLKVGVRTRGCNGLSYSLEYTKTKGDSDEEVIQDGVRVFIEKKAQLTLLGTEMDYV 102
Cdd:COG0316   1 PITLTDAAAKRIKRLLaKEGNPGLGLRVGVKGGGCSGFSYGLDFDDEPNEDDLVFEQDGVKVVVDPKSLPYLDGTEIDYV 80

                        90       100
                ....*....|....*....|....*..
gi 21312189 103 EDKLSSEFVFNNPNIKGTCGCGESFHV 129
Cdd:COG0316  81 EELLGSGFKFNNPNAKSSCGCGESFSV 107
 
Name Accession Description Interval E-value
IscA COG0316
Fe-S cluster assembly iron-binding protein IscA [Posttranslational modification, protein ...
 24-129 3.16e-47

Fe-S cluster assembly iron-binding protein IscA [Posttranslational modification, protein turnover, chaperones];


Pssm-ID: 440085 [Multi-domain]  Cd Length: 107  Bit Score: 147.61  E-value: 3.16e-47
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 21312189  24 ALTLTPSAVNKIKQLL-KDKPEHVGLKVGVRTRGCNGLSYSLEYTKTKGDSDEEVIQDGVRVFIEKKAQLTLLGTEMDYV 102
Cdd:COG0316   1 PITLTDAAAKRIKRLLaKEGNPGLGLRVGVKGGGCSGFSYGLDFDDEPNEDDLVFEQDGVKVVVDPKSLPYLDGTEIDYV 80

                        90       100
                ....*....|....*....|....*..
gi 21312189 103 EDKLSSEFVFNNPNIKGTCGCGESFHV 129
Cdd:COG0316  81 EELLGSGFKFNNPNAKSSCGCGESFSV 107
TIGR00049 TIGR00049
Iron-sulfur cluster assembly accessory protein; Proteins in this subfamily appear to be ...
 26-129 8.80e-46

Iron-sulfur cluster assembly accessory protein; Proteins in this subfamily appear to be associated with the process of FeS-cluster assembly. The HesB proteins are associated with the nif gene cluster and the Rhizobium gene IscN has been shown to be required for nitrogen fixation. Nitrogenase includes multiple FeS clusters and many genes for their assembly. The E. coli SufA protein is associated with SufS, a NifS homolog and SufD which are involved in the FeS cluster assembly of the FhnF protein. The Azotobacter protein IscA (homologs of which are also found in E.coli) is associated which IscS, another NifS homolog and IscU, a nifU homolog as well as other factors consistent with a role in FeS cluster chemistry. A homolog from Geobacter contains a selenocysteine in place of an otherwise invariant cysteine, further suggesting a role in redox chemistry. [Biosynthesis of cofactors, prosthetic groups, and carriers, Other]


Pssm-ID: 272875 [Multi-domain]  Cd Length: 105  Bit Score: 143.87  E-value: 8.80e-46
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 21312189    26 TLTPSAVNKIKQLLKDKPEHV-GLKVGVRTRGCNGLSYSLEYTKTKGDSDEEVIQDGVRVFIEKKAQLTLLGTEMDYVED 104
Cdd:TIGR00049   1 TLTDSAAKRIKALLAGEGEPNlGLRVGVKGGGCSGLQYGLEFDDEPNEDDEVFEQDGVKVVVDPKSLPYLDGSEIDYVEE 80

                          90       100
                  ....*....|....*....|....*
gi 21312189   105 KLSSEFVFNNPNIKGTCGCGESFHV 129
Cdd:TIGR00049  81 LLGSGFTFTNPNAKGTCGCGKSFSV 105
sufA PRK09504
iron-sulfur cluster assembly scaffold protein; Provisional
 25-129 3.96e-29

iron-sulfur cluster assembly scaffold protein; Provisional


Pssm-ID: 181915  Cd Length: 122  Bit Score: 102.12  E-value: 3.96e-29
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 21312189   25 LTLTPSAVNKIKQLLKDKPEHVGLKVGVRTRGCNGLSYSLEYTKTKGDSDEEVIQDGVRVFIEKKAQLTLLGTEMDYVED 104
Cdd:PRK09504  18 LTLTPAAAAHIRELMAKQPGMKGVRLGVKQTGCAGFGYVLDSVSEPDKDDLVFEHDGAKLFVPLQAMPFIDGTEVDYVRE 97

                         90       100
                 ....*....|....*....|....*
gi 21312189  105 KLSSEFVFNNPNIKGTCGCGESFHV 129
Cdd:PRK09504  98 GLNQIFKFHNPKAQNECGCGESFGV 122
Fe-S_biosyn pfam01521
Iron-sulphur cluster biosynthesis; This family is involved in iron-sulphur cluster ...
 25-125 7.56e-19

Iron-sulphur cluster biosynthesis; This family is involved in iron-sulphur cluster biosynthesis. Its members include proteins that are involved in nitrogen fixation such as the HesB and HesB-like proteins.


Pssm-ID: 426304  Cd Length: 111  Bit Score: 75.76  E-value: 7.56e-19
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 21312189    25 LTLTPSAVNKIKQLLKDKPEHVGLKV----GVRTRGC--NGLSYSLEY-TKTKGDSDEEVIQDGVRVFIeKKAQLTLL-- 95
Cdd:pfam01521   3 ITITDAAAERLKKLLAGDKKELRLDVddggGPYSKGGcsIGGKFSLVLvDEPDPDYDEVIESNGGPIYV-DSYSLPFLde 81

                          90       100       110
                  ....*....|....*....|....*....|
gi 21312189    96 GTEMDYVEDKLSSEFVFNNPNIKGTCGCGE 125
Cdd:pfam01521  82 GLTLDFVEDLGTLGLKSDNGNLDGNVGCGD 111
IscA_HesB_Se NF038090
IscA/HesB family protein; Members of this family, a large fraction of which are selenoproteins, ...
 25-123 3.79e-05

IscA/HesB family protein; Members of this family, a large fraction of which are selenoproteins, are homologous to proteins of iron-sulfur cluster biosynthesis such as IscA, and belong to the broader set of HesB-related proteins.


Pssm-ID: 411672  Cd Length: 99  Bit Score: 40.00  E-value: 3.79e-05
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 21312189   25 LTLTPSAVNKIKQLLKDKPEHVGLKVGVRTRGCNGLSYSLEYTKTKgDSDEEVIQDGVRVFIEKKaqltLLGT----EMD 100
Cdd:NF038090   2 LEVTDLASRKLKEYFADKKIDSPIRVFLNQGGUAGPSLGLALDEPK-ENDEVFEQDGLTFVIDKE----LLEQcgpiKVD 76

                         90       100
                 ....*....|....*....|...
gi 21312189  101 YVEDKLSSEFVFNNPNIKGTCGC 123
Cdd:NF038090  77 FIDCRSGFSITSSMPLGGGGCGS 99
 
Name Accession Description Interval E-value
IscA COG0316
Fe-S cluster assembly iron-binding protein IscA [Posttranslational modification, protein ...
 24-129 3.16e-47

Fe-S cluster assembly iron-binding protein IscA [Posttranslational modification, protein turnover, chaperones];


Pssm-ID: 440085 [Multi-domain]  Cd Length: 107  Bit Score: 147.61  E-value: 3.16e-47
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 21312189  24 ALTLTPSAVNKIKQLL-KDKPEHVGLKVGVRTRGCNGLSYSLEYTKTKGDSDEEVIQDGVRVFIEKKAQLTLLGTEMDYV 102
Cdd:COG0316   1 PITLTDAAAKRIKRLLaKEGNPGLGLRVGVKGGGCSGFSYGLDFDDEPNEDDLVFEQDGVKVVVDPKSLPYLDGTEIDYV 80

                        90       100
                ....*....|....*....|....*..
gi 21312189 103 EDKLSSEFVFNNPNIKGTCGCGESFHV 129
Cdd:COG0316  81 EELLGSGFKFNNPNAKSSCGCGESFSV 107
TIGR00049 TIGR00049
Iron-sulfur cluster assembly accessory protein; Proteins in this subfamily appear to be ...
 26-129 8.80e-46

Iron-sulfur cluster assembly accessory protein; Proteins in this subfamily appear to be associated with the process of FeS-cluster assembly. The HesB proteins are associated with the nif gene cluster and the Rhizobium gene IscN has been shown to be required for nitrogen fixation. Nitrogenase includes multiple FeS clusters and many genes for their assembly. The E. coli SufA protein is associated with SufS, a NifS homolog and SufD which are involved in the FeS cluster assembly of the FhnF protein. The Azotobacter protein IscA (homologs of which are also found in E.coli) is associated which IscS, another NifS homolog and IscU, a nifU homolog as well as other factors consistent with a role in FeS cluster chemistry. A homolog from Geobacter contains a selenocysteine in place of an otherwise invariant cysteine, further suggesting a role in redox chemistry. [Biosynthesis of cofactors, prosthetic groups, and carriers, Other]


Pssm-ID: 272875 [Multi-domain]  Cd Length: 105  Bit Score: 143.87  E-value: 8.80e-46
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 21312189    26 TLTPSAVNKIKQLLKDKPEHV-GLKVGVRTRGCNGLSYSLEYTKTKGDSDEEVIQDGVRVFIEKKAQLTLLGTEMDYVED 104
Cdd:TIGR00049   1 TLTDSAAKRIKALLAGEGEPNlGLRVGVKGGGCSGLQYGLEFDDEPNEDDEVFEQDGVKVVVDPKSLPYLDGSEIDYVEE 80

                          90       100
                  ....*....|....*....|....*
gi 21312189   105 KLSSEFVFNNPNIKGTCGCGESFHV 129
Cdd:TIGR00049  81 LLGSGFTFTNPNAKGTCGCGKSFSV 105
sufA_proteo TIGR01997
FeS assembly scaffold SufA; This model represents the SufA protein of the SUF system of ...
 23-129 1.52e-34

FeS assembly scaffold SufA; This model represents the SufA protein of the SUF system of iron-sulfur cluster biosynthesis. This system performs FeS biosynthesis even during oxidative stress and tends to be absent in obligate anaerobic and microaerophilic bacteria. [Biosynthesis of cofactors, prosthetic groups, and carriers, Other]


Pssm-ID: 131052  Cd Length: 107  Bit Score: 115.70  E-value: 1.52e-34
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 21312189    23 AALTLTPSAVNKIKQLLKDKPEHVGLKVGVRTRGCNGLSYSLEYTKTKGDSDEEVIQDGVRVFIEKKAQLTLLGTEMDYV 102
Cdd:TIGR01997   1 AVITLTDAAAIHIRELVAKRPEAVGIRLGVKKTGCAGMEYVLDLVSEPKKDDDLIEHDGAKVFVAPEAVLFILGTQVDFV 80

                          90       100
                  ....*....|....*....|....*..
gi 21312189   103 EDKLSSEFVFNNPNIKGTCGCGESFHV 129
Cdd:TIGR01997  81 RTTLRQGFKFNNPNATSACGCGESFEL 107
IscA TIGR02011
iron-sulfur cluster assembly protein IscA; This model represents the IscA component of the ISC ...
 25-129 8.41e-32

iron-sulfur cluster assembly protein IscA; This model represents the IscA component of the ISC system for iron-sulfur cluster assembly. The ISC system consists of IscRASU, HscAB and an Isc-specific ferredoxin. IscA previously was believed to act as a scaffold and now is seen as an iron donor protein. This clade is limited to the proteobacteria. [Biosynthesis of cofactors, prosthetic groups, and carriers, Other]


Pssm-ID: 213674  Cd Length: 105  Bit Score: 108.37  E-value: 8.41e-32
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 21312189    25 LTLTPSAVNKIKQLLKDKPEHVGLKVGVRTRGCNGLSYSLEYTKTKGDSDEEVIQDGVRVFIEKKAQLTLLGTEMDYVED 104
Cdd:TIGR02011   1 ITLTDSAAARVNTFLANRGKGFGLRLGVKTSGCSGMAYVLEFVDEPTPDDIVFEDKGVKIVIDGKSLQYLDGTQLDFVKE 80

                          90       100
                  ....*....|....*....|....*
gi 21312189   105 KLSSEFVFNNPNIKGTCGCGESFHV 129
Cdd:TIGR02011  81 GLNEGFKFTNPNVKDECGCGESFHV 105
sufA PRK09504
iron-sulfur cluster assembly scaffold protein; Provisional
 25-129 3.96e-29

iron-sulfur cluster assembly scaffold protein; Provisional


Pssm-ID: 181915  Cd Length: 122  Bit Score: 102.12  E-value: 3.96e-29
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 21312189   25 LTLTPSAVNKIKQLLKDKPEHVGLKVGVRTRGCNGLSYSLEYTKTKGDSDEEVIQDGVRVFIEKKAQLTLLGTEMDYVED 104
Cdd:PRK09504  18 LTLTPAAAAHIRELMAKQPGMKGVRLGVKQTGCAGFGYVLDSVSEPDKDDLVFEHDGAKLFVPLQAMPFIDGTEVDYVRE 97

                         90       100
                 ....*....|....*....|....*
gi 21312189  105 KLSSEFVFNNPNIKGTCGCGESFHV 129
Cdd:PRK09504  98 GLNQIFKFHNPKAQNECGCGESFGV 122
iscA PRK09502
iron-sulfur cluster assembly protein IscA;
24-129 4.73e-29

iron-sulfur cluster assembly protein IscA;


Pssm-ID: 181914 [Multi-domain]  Cd Length: 107  Bit Score: 101.49  E-value: 4.73e-29
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 21312189   24 ALTLTPSAVNKIKQLLKDKPEHVGLKVGVRTRGCNGLSYSLEYTKTKGDSDEEVIQDGVRVFIEKKAQLTLLGTEMDYVE 103
Cdd:PRK09502   2 SITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEFVDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVK 81

                         90       100
                 ....*....|....*....|....*.
gi 21312189  104 DKLSSEFVFNNPNIKGTCGCGESFHV 129
Cdd:PRK09502  82 EGLNEGFKFTNPNVKDECGCGESFHV 107
PRK13623 PRK13623
iron-sulfur cluster insertion protein ErpA; Provisional
 19-129 3.02e-21

iron-sulfur cluster insertion protein ErpA; Provisional


Pssm-ID: 184186  Cd Length: 115  Bit Score: 81.90  E-value: 3.02e-21
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 21312189   19 QPTRAALTLTPSAVNKIKQLLKD-KPEHVGLKVGVRTRGCNGLSYSLEYTKTKGDSDEEVIQDGVRVFIEKKAQLTLLGT 97
Cdd:PRK13623   4 DDVPLPLVFTDAAAAKVKELIEEeGNPDLKLRVYITGGGCSGFQYGFTFDEQVNEDDTTIEKQGVTLVVDPMSLQYLVGA 83

                         90       100       110
                 ....*....|....*....|....*....|..
gi 21312189   98 EMDYVEDKLSSEFVFNNPNIKGTCGCGESFHV 129
Cdd:PRK13623  84 EVDYTEGLEGSRFVIKNPNAKTTCGCGSSFSI 115
Fe-S_biosyn pfam01521
Iron-sulphur cluster biosynthesis; This family is involved in iron-sulphur cluster ...
 25-125 7.56e-19

Iron-sulphur cluster biosynthesis; This family is involved in iron-sulphur cluster biosynthesis. Its members include proteins that are involved in nitrogen fixation such as the HesB and HesB-like proteins.


Pssm-ID: 426304  Cd Length: 111  Bit Score: 75.76  E-value: 7.56e-19
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 21312189    25 LTLTPSAVNKIKQLLKDKPEHVGLKV----GVRTRGC--NGLSYSLEY-TKTKGDSDEEVIQDGVRVFIeKKAQLTLL-- 95
Cdd:pfam01521   3 ITITDAAAERLKKLLAGDKKELRLDVddggGPYSKGGcsIGGKFSLVLvDEPDPDYDEVIESNGGPIYV-DSYSLPFLde 81

                          90       100       110
                  ....*....|....*....|....*....|
gi 21312189    96 GTEMDYVEDKLSSEFVFNNPNIKGTCGCGE 125
Cdd:pfam01521  82 GLTLDFVEDLGTLGLKSDNGNLDGNVGCGD 111
PLN03082 PLN03082
Iron-sulfur cluster assembly; Provisional
 2-129 1.32e-13

Iron-sulfur cluster assembly; Provisional


Pssm-ID: 215564  Cd Length: 163  Bit Score: 63.41  E-value: 1.32e-13
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 21312189    2 SASLVRATVRAVSKRKLQPTRAALTLTPSAVNKIKQLLKDKPEHVG--LKVGVRTRGCNGLSYSLEYtKTKGDSDEEVIQ 79
Cdd:PLN03082  32 SSSALKEASTSSSSPSSSASLDAVHMTDNCIRRLKELQTSEPSAEDkmLRLSVETGGCSGFQYVFEL-DDKTNSDDRVFE 110

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|..
gi 21312189   80 -DGVRVFIEKKAQLTLLGTEMDYVEDKLSSEFVFN-NPNIKGTCGCGESFHV 129
Cdd:PLN03082 111 kDGVKLVVDNISYDFVKGATVDYVEELIRSAFVVStNPSAVGGCSCKSSFMV 162
IscA_HesB_Se NF038090
IscA/HesB family protein; Members of this family, a large fraction of which are selenoproteins, ...
 25-123 3.79e-05

IscA/HesB family protein; Members of this family, a large fraction of which are selenoproteins, are homologous to proteins of iron-sulfur cluster biosynthesis such as IscA, and belong to the broader set of HesB-related proteins.


Pssm-ID: 411672  Cd Length: 99  Bit Score: 40.00  E-value: 3.79e-05
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 21312189   25 LTLTPSAVNKIKQLLKDKPEHVGLKVGVRTRGCNGLSYSLEYTKTKgDSDEEVIQDGVRVFIEKKaqltLLGT----EMD 100
Cdd:NF038090   2 LEVTDLASRKLKEYFADKKIDSPIRVFLNQGGUAGPSLGLALDEPK-ENDEVFEQDGLTFVIDKE----LLEQcgpiKVD 76

                         90       100
                 ....*....|....*....|...
gi 21312189  101 YVEDKLSSEFVFNNPNIKGTCGC 123
Cdd:NF038090  77 FIDCRSGFSITSSMPLGGGGCGS 99
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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