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Conserved domains on  [gi|13386066|ref|NP_080848|]
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glycine cleavage system H protein, mitochondrial precursor [Mus musculus]

Protein Classification

glycine cleavage system protein H( domain architecture ID 10785558)

glycine cleavage system protein H plays a role in the degradation of glycine by shuttling the methylamine group of glycine from P-protein (glycine dehydrogenase) to the T-protein (aminomethyltransferase).

EC:  1.4.1.27
Gene Ontology:  GO:0019464|GO:0009249|GO:0005960
PubMed:  20375021|18941301

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
GcvH COG0509
Glycine cleavage system protein H (lipoate-binding) [Amino acid transport and metabolism]; ...
 48-167 2.09e-65

Glycine cleavage system protein H (lipoate-binding) [Amino acid transport and metabolism]; Glycine cleavage system protein H (lipoate-binding) is part of the Pathway/BioSystem: Glycine cleavage


:

Pssm-ID: 440275  Cd Length: 128  Bit Score: 196.11  E-value: 2.09e-65
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 13386066  48 RKFTEKHEWITTE-EGIGTVGISNFAQEALGDVVYCSLPEVGTKLKKQEEFGALESVKAASELYSPLSGEVTEVNEALAE 126
Cdd:COG0509   7 LKYTEDHEWVRVEgDGTATVGITDFAQDLLGDIVFVELPEVGTEVEAGEPFGVVESVKAVSDLYAPVSGEVVEVNEALED 86

                        90       100       110       120
                ....*....|....*....|....*....|....*....|.
gi 13386066 127 NPGLVNKSCYEDGWLIKMTLSDPSEMDELMSEEAYEKYVKS 167
Cdd:COG0509  87 DPELVNEDPYGEGWLFKIKPSDPAELDDLLDAEAYAALLEE 127
 
Name Accession Description Interval E-value
GcvH COG0509
Glycine cleavage system protein H (lipoate-binding) [Amino acid transport and metabolism]; ...
 48-167 2.09e-65

Glycine cleavage system protein H (lipoate-binding) [Amino acid transport and metabolism]; Glycine cleavage system protein H (lipoate-binding) is part of the Pathway/BioSystem: Glycine cleavage


Pssm-ID: 440275  Cd Length: 128  Bit Score: 196.11  E-value: 2.09e-65
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 13386066  48 RKFTEKHEWITTE-EGIGTVGISNFAQEALGDVVYCSLPEVGTKLKKQEEFGALESVKAASELYSPLSGEVTEVNEALAE 126
Cdd:COG0509   7 LKYTEDHEWVRVEgDGTATVGITDFAQDLLGDIVFVELPEVGTEVEAGEPFGVVESVKAVSDLYAPVSGEVVEVNEALED 86

                        90       100       110       120
                ....*....|....*....|....*....|....*....|.
gi 13386066 127 NPGLVNKSCYEDGWLIKMTLSDPSEMDELMSEEAYEKYVKS 167
Cdd:COG0509  87 DPELVNEDPYGEGWLFKIKPSDPAELDDLLDAEAYAALLEE 127
PRK01202 PRK01202
glycine cleavage system protein GcvH;
48-167 2.27e-64

glycine cleavage system protein GcvH;


Pssm-ID: 234918  Cd Length: 127  Bit Score: 193.45  E-value: 2.27e-64
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 13386066   48 RKFTEKHEWITTEEGIGTVGISNFAQEALGDVVYCSLPEVGTKLKKQEEFGALESVKAASELYSPLSGEVTEVNEALAEN 127
Cdd:PRK01202   8 LKYTKSHEWVRVEGDTATVGITDHAQEQLGDIVFVELPEVGDEVKAGETFGVVESVKAASDIYAPVSGEVVEVNEALEDS 87

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|
gi 13386066  128 PGLVNKSCYEDGWLIKMTLSDPSEMDELMSEEAYEKYVKS 167
Cdd:PRK01202  88 PELVNEDPYGEGWLFKIKPSDESELDDLLDAEAYEALIEE 127
GCV_H pfam01597
Glycine cleavage H-protein; This is a family of glycine cleavage H-proteins, part of the ...
 48-168 2.02e-61

Glycine cleavage H-protein; This is a family of glycine cleavage H-proteins, part of the glycine cleavage multienzyme complex (GCV) found in bacteria and the mitochondria of eukaryotes. GCV catalyzes the catabolism of glycine in eukaryotes. A lipoyl group is attached to a completely conserved lysine residue. The H protein shuttles the methylamine group of glycine from the P protein to the T protein.


Pssm-ID: 396258  Cd Length: 122  Bit Score: 185.61  E-value: 2.02e-61
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 13386066    48 RKFTEKHEWITTEE-GIGTVGISNFAQEALGDVVYCSLPEVGTKLKKQEEFGALESVKAASELYSPLSGEVTEVNEALAE 126
Cdd:pfam01597   1 LYYTKEHEWVKVEGdGTATVGITDFAQAQLGDIVFVELPEVGTKVKKGESLAAIESVKAASPIYAPVSGEVVEVNEKLED 80

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|..
gi 13386066   127 NPGLVNKSCYEDGWLIKMTLSDPSEMDELMSEEAYEKYVKSI 168
Cdd:pfam01597  81 NPGLINKDPYEDGWIAKLKPSNLEELESLMTAEQYEKTLKEI 122
GCS_H cd06848
Glycine cleavage H-protein. Glycine cleavage H-proteins are part of the glycine cleavage ...
 50-144 1.10e-49

Glycine cleavage H-protein. Glycine cleavage H-proteins are part of the glycine cleavage system (GCS) found in bacteria, archea and the mitochondria of eukaryotes. GCS is a multienzyme complex consisting of 4 different components (P-, H-, T- and L-proteins) which catalyzes the oxidative cleavage of glycine. The H-protein shuttles the methylamine group of glycine from the P-protein (glycine dehydrogenase) to the T-protein (aminomethyltransferase) via a lipoyl group, attached to a completely conserved lysine residue.


Pssm-ID: 133457 [Multi-domain]  Cd Length: 96  Bit Score: 155.00  E-value: 1.10e-49
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 13386066  50 FTEKHEWITTEE-GIGTVGISNFAQEALGDVVYCSLPEVGTKLKKQEEFGALESVKAASELYSPLSGEVTEVNEALAENP 128
Cdd:cd06848   1 YTKDHEWVKVEGdGIATVGITDYAQDLLGDIVFVELPEVGTEVKKGDPFGSVESVKAASDLYSPVSGEVVEVNEALLDNP 80

                        90
                ....*....|....*.
gi 13386066 129 GLVNKSCYEDGWLIKM 144
Cdd:cd06848  81 ELINSDPYGEGWLVKI 96
gcvH TIGR00527
glycine cleavage system H protein; This model represents the glycine cleavage system H protein, ...
 49-163 2.48e-45

glycine cleavage system H protein; This model represents the glycine cleavage system H protein, which shuttles the methylamine group of glycine from the P protein to the T protein. The mature protein is about 130 residues long and contains a lipoyl group covalently bound to a conserved Lys residue. The genome of Aquifex aeolicus contains one protein scoring above the trusted cutoff and clustering with other bacterial H proteins, and four more proteins clustering together and scoring below the trusted cutoff; it seems doubtful that all of these homologs are authentic H protein. The Chlamydial homolog of H protein is nearly as divergent as the Aquifex outgroup, is not accompanied by P and T proteins, is not included in the seed alignment, and consequently also scores below the trusted cutoff. [Energy metabolism, Amino acids and amines]


Pssm-ID: 200024  Cd Length: 128  Bit Score: 145.36  E-value: 2.48e-45
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 13386066    49 KFTEKHEWITTE-EGIGTVGISNFAQEALGDVVYCSLPEVGTKLKKQEEFGALESVKAASELYSPLSGEVTEVNEALAEN 127
Cdd:TIGR00527   7 RYSSEHEWVRVEgDKTATVGITKFAQDELGDIVFVELPEVGAEVAAGESLGSVESVKAASDIYAPVDGTVVEVNTALEDS 86

                          90       100       110
                  ....*....|....*....|....*....|....*..
gi 13386066   128 PGLVNKSCYEDGWLIKMTLSDP-SEMDELMSEEAYEK 163
Cdd:TIGR00527  87 PELVNEDPYGDGWLIKVKLSDGeSEVEGLMSAEQYEA 123
 
Name Accession Description Interval E-value
GcvH COG0509
Glycine cleavage system protein H (lipoate-binding) [Amino acid transport and metabolism]; ...
 48-167 2.09e-65

Glycine cleavage system protein H (lipoate-binding) [Amino acid transport and metabolism]; Glycine cleavage system protein H (lipoate-binding) is part of the Pathway/BioSystem: Glycine cleavage


Pssm-ID: 440275  Cd Length: 128  Bit Score: 196.11  E-value: 2.09e-65
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 13386066  48 RKFTEKHEWITTE-EGIGTVGISNFAQEALGDVVYCSLPEVGTKLKKQEEFGALESVKAASELYSPLSGEVTEVNEALAE 126
Cdd:COG0509   7 LKYTEDHEWVRVEgDGTATVGITDFAQDLLGDIVFVELPEVGTEVEAGEPFGVVESVKAVSDLYAPVSGEVVEVNEALED 86

                        90       100       110       120
                ....*....|....*....|....*....|....*....|.
gi 13386066 127 NPGLVNKSCYEDGWLIKMTLSDPSEMDELMSEEAYEKYVKS 167
Cdd:COG0509  87 DPELVNEDPYGEGWLFKIKPSDPAELDDLLDAEAYAALLEE 127
PRK01202 PRK01202
glycine cleavage system protein GcvH;
48-167 2.27e-64

glycine cleavage system protein GcvH;


Pssm-ID: 234918  Cd Length: 127  Bit Score: 193.45  E-value: 2.27e-64
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 13386066   48 RKFTEKHEWITTEEGIGTVGISNFAQEALGDVVYCSLPEVGTKLKKQEEFGALESVKAASELYSPLSGEVTEVNEALAEN 127
Cdd:PRK01202   8 LKYTKSHEWVRVEGDTATVGITDHAQEQLGDIVFVELPEVGDEVKAGETFGVVESVKAASDIYAPVSGEVVEVNEALEDS 87

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|
gi 13386066  128 PGLVNKSCYEDGWLIKMTLSDPSEMDELMSEEAYEKYVKS 167
Cdd:PRK01202  88 PELVNEDPYGEGWLFKIKPSDESELDDLLDAEAYEALIEE 127
GCV_H pfam01597
Glycine cleavage H-protein; This is a family of glycine cleavage H-proteins, part of the ...
 48-168 2.02e-61

Glycine cleavage H-protein; This is a family of glycine cleavage H-proteins, part of the glycine cleavage multienzyme complex (GCV) found in bacteria and the mitochondria of eukaryotes. GCV catalyzes the catabolism of glycine in eukaryotes. A lipoyl group is attached to a completely conserved lysine residue. The H protein shuttles the methylamine group of glycine from the P protein to the T protein.


Pssm-ID: 396258  Cd Length: 122  Bit Score: 185.61  E-value: 2.02e-61
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 13386066    48 RKFTEKHEWITTEE-GIGTVGISNFAQEALGDVVYCSLPEVGTKLKKQEEFGALESVKAASELYSPLSGEVTEVNEALAE 126
Cdd:pfam01597   1 LYYTKEHEWVKVEGdGTATVGITDFAQAQLGDIVFVELPEVGTKVKKGESLAAIESVKAASPIYAPVSGEVVEVNEKLED 80

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|..
gi 13386066   127 NPGLVNKSCYEDGWLIKMTLSDPSEMDELMSEEAYEKYVKSI 168
Cdd:pfam01597  81 NPGLINKDPYEDGWIAKLKPSNLEELESLMTAEQYEKTLKEI 122
GCS_H cd06848
Glycine cleavage H-protein. Glycine cleavage H-proteins are part of the glycine cleavage ...
 50-144 1.10e-49

Glycine cleavage H-protein. Glycine cleavage H-proteins are part of the glycine cleavage system (GCS) found in bacteria, archea and the mitochondria of eukaryotes. GCS is a multienzyme complex consisting of 4 different components (P-, H-, T- and L-proteins) which catalyzes the oxidative cleavage of glycine. The H-protein shuttles the methylamine group of glycine from the P-protein (glycine dehydrogenase) to the T-protein (aminomethyltransferase) via a lipoyl group, attached to a completely conserved lysine residue.


Pssm-ID: 133457 [Multi-domain]  Cd Length: 96  Bit Score: 155.00  E-value: 1.10e-49
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 13386066  50 FTEKHEWITTEE-GIGTVGISNFAQEALGDVVYCSLPEVGTKLKKQEEFGALESVKAASELYSPLSGEVTEVNEALAENP 128
Cdd:cd06848   1 YTKDHEWVKVEGdGIATVGITDYAQDLLGDIVFVELPEVGTEVKKGDPFGSVESVKAASDLYSPVSGEVVEVNEALLDNP 80

                        90
                ....*....|....*.
gi 13386066 129 GLVNKSCYEDGWLIKM 144
Cdd:cd06848  81 ELINSDPYGEGWLVKI 96
gcvH TIGR00527
glycine cleavage system H protein; This model represents the glycine cleavage system H protein, ...
 49-163 2.48e-45

glycine cleavage system H protein; This model represents the glycine cleavage system H protein, which shuttles the methylamine group of glycine from the P protein to the T protein. The mature protein is about 130 residues long and contains a lipoyl group covalently bound to a conserved Lys residue. The genome of Aquifex aeolicus contains one protein scoring above the trusted cutoff and clustering with other bacterial H proteins, and four more proteins clustering together and scoring below the trusted cutoff; it seems doubtful that all of these homologs are authentic H protein. The Chlamydial homolog of H protein is nearly as divergent as the Aquifex outgroup, is not accompanied by P and T proteins, is not included in the seed alignment, and consequently also scores below the trusted cutoff. [Energy metabolism, Amino acids and amines]


Pssm-ID: 200024  Cd Length: 128  Bit Score: 145.36  E-value: 2.48e-45
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 13386066    49 KFTEKHEWITTE-EGIGTVGISNFAQEALGDVVYCSLPEVGTKLKKQEEFGALESVKAASELYSPLSGEVTEVNEALAEN 127
Cdd:TIGR00527   7 RYSSEHEWVRVEgDKTATVGITKFAQDELGDIVFVELPEVGAEVAAGESLGSVESVKAASDIYAPVDGTVVEVNTALEDS 86

                          90       100       110
                  ....*....|....*....|....*....|....*..
gi 13386066   128 PGLVNKSCYEDGWLIKMTLSDP-SEMDELMSEEAYEK 163
Cdd:TIGR00527  87 PELVNEDPYGDGWLIKVKLSDGeSEVEGLMSAEQYEA 123
PRK13380 PRK13380
glycine cleavage system protein H; Provisional
 48-170 3.95e-40

glycine cleavage system protein H; Provisional


Pssm-ID: 237370  Cd Length: 144  Bit Score: 132.43  E-value: 3.95e-40
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 13386066   48 RKFTEKHEWITTEE-GIGTVGISNFAQEALGDVVYCSLPEVGTKLKKQEEFGALESVKAASELYSPLSGEVTEVNEALAE 126
Cdd:PRK13380  14 RYTIKNHEWLRLEGdGTVTVGITDYAQTMAGDVVFVRLKELGKKVEKGKPVATLESGKWAGPVPAPLTGEVVEVNEALED 93

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....
gi 13386066  127 NPGLVNKSCYEDGWLIKMTLSDPSEMDELMSEEAYEKYVKSIEE 170
Cdd:PRK13380  94 SPELVNEDPYGEGWFFRFKPANPEELKQLLDGDAADRLLKENID 137
PRK00624 PRK00624
glycine cleavage system protein H; Provisional
 50-148 1.86e-18

glycine cleavage system protein H; Provisional


Pssm-ID: 167014  Cd Length: 114  Bit Score: 76.02  E-value: 1.86e-18
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 13386066   50 FTEKHEWIT-TEEGIGTVGISNFAQEALGDVVYCSLPEVGTKLKKQEEFGALESVKAASELYSPLSGEVTEVNEALAENP 128
Cdd:PRK00624   4 YSDYHVWIEpIHSRIVRLGLTSKMQENLGNILHIDLPSVGSFCKEGEVLVILESSKSAIEVLSPVSGEVIEVNTALEDDI 83

                         90       100
                 ....*....|....*....|
gi 13386066  129 GLVNKSCYEDGWLIKMTLSD 148
Cdd:PRK00624  84 QPINNAPESEGWFVVVQLDE 103
Biotinyl_lipoyl_domains cd06663
Biotinyl_lipoyl_domains are present in biotin-dependent carboxylases/decarboxylases, the ...
 65-143 6.97e-12

Biotinyl_lipoyl_domains are present in biotin-dependent carboxylases/decarboxylases, the dihydrolipoyl acyltransferase component (E2) of 2-oxo acid dehydrogenases, and the H-protein of the glycine cleavage system (GCS). These domains transport CO2, acyl, or methylamine, respectively, between components of the complex/protein via a biotinyl or lipoyl group, which is covalently attached to a highly conserved lysine residue.


Pssm-ID: 133456 [Multi-domain]  Cd Length: 73  Bit Score: 57.84  E-value: 6.97e-12
                        10        20        30        40        50        60        70
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 13386066  65 TVGISNFAQEALGDVVYCSLPEVGTKLKKQEEFGALESVKAASELYSPLSGEVTEVNEALAENPglvnkscYEDGWLIK 143
Cdd:cd06663   1 TILIPDLAQHLGDGTVVKWLKKVGDKVKKGDVLAEIEAMKATSDVEAPKSGTVKKVLVKEGTKV-------EGDTPLVK 72
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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