Pre-mRNA splicing factor; This domain family is found in eukaryotes, and is approximately 100 amino acids in length. The family is found in association with pfam10197. There is a single completely conserved residue Y that may be functionally important. Cwc25 has been identified to associate with pre-mRNA splicing factor Cef1/Ntc85, a component of the Prp19-associated complex (NTC) involved in spliceosome activation. Cwc25 is neither tightly associated with NTC nor required for spliceosome activation, but is required for the first catalytic reaction.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 262072988   67 EEKLDWMYQGPGGMVNR--DEYLLGRP-IDKYVFEK---MEEREAGCSSETGLLPGSIFAPSGANSLLDMASKIREDPLF 140
Cdd:pfam12542   1 LERLDWMYDGPSGGVEEemEEYLLGKKrVDKLLEGGddaEKKKAAKASFSAASVPGALFLEKAINSARDTARKLREDPLL 80

                          90
                  ....*....|....*..
gi 262072988  141 IIRKKEEEKKREVLNNP 157
Cdd:pfam12542  81 AIKKQEQEARQALLNNP 97

Atrophin-1 family; Atrophin-1 is the protein product of the dentatorubral-pallidoluysian atrophy (DRPLA) gene. DRPLA OMIM:125370 is a progressive neurodegenerative disorder. It is caused by the expansion of a CAG repeat in the DRPLA gene on chromosome 12p. This results in an extended polyglutamine region in atrophin-1, that is thought to confer toxicity to the protein, possibly through altering its interactions with other proteins. The expansion of a CAG repeat is also the underlying defect in six other neurodegenerative disorders, including Huntington's disease. One interaction of expanded polyglutamine repeats that is thought to be pathogenic is that with the short glutamine repeat in the transcriptional coactivator CREB binding protein, CBP. This interaction draws CBP away from its usual nuclear location to the expanded polyglutamine repeat protein aggregates that are characteriztic of the polyglutamine neurodegenerative disorders. This interferes with CBP-mediated transcription and causes cytotoxicity.

                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 262072988  323 YTRKLSAEELERKRQEMMENAKWREEERLNTLKRHAREDEREHRLERLDSRSGKflhrmKLESASTSSLEDRV 395
Cdd:pfam03154 576 YFMPLAGSKLAKKREEALEKAKREAEQKAREEKEREKEKEKEREREREREREAE-----RAAKASSSSHEGRM 643

Pre-mRNA splicing factor; This domain family is found in eukaryotes, and is approximately 100 amino acids in length. The family is found in association with pfam10197. There is a single completely conserved residue Y that may be functionally important. Cwc25 has been identified to associate with pre-mRNA splicing factor Cef1/Ntc85, a component of the Prp19-associated complex (NTC) involved in spliceosome activation. Cwc25 is neither tightly associated with NTC nor required for spliceosome activation, but is required for the first catalytic reaction.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 262072988   67 EEKLDWMYQGPGGMVNR--DEYLLGRP-IDKYVFEK---MEEREAGCSSETGLLPGSIFAPSGANSLLDMASKIREDPLF 140
Cdd:pfam12542   1 LERLDWMYDGPSGGVEEemEEYLLGKKrVDKLLEGGddaEKKKAAKASFSAASVPGALFLEKAINSARDTARKLREDPLL 80

                          90
                  ....*....|....*..
gi 262072988  141 IIRKKEEEKKREVLNNP 157
Cdd:pfam12542  81 AIKKQEQEARQALLNNP 97

N-terminal domain of CBF1 interacting co-repressor CIR; This is a 45 residue conserved region at the N-terminal end of a family of proteins referred to as CIRs (CBF1-interacting co-repressors). CBF1 (centromere-binding factor 1) acts as a transcription factor that causes repression by binding specifically to GTGGGAA motifs in responsive promoters, and it requires CIR as a co-repressor. CIR binds to histone deacetylase and to SAP30 and serves as a linker between CBF1 and the histone deacetylase complex.

                           10        20
                   ....*....|....*....|....*..
gi 262072988    11 SWHPQTLRNVEKVWKAEQKHEAERKKI 37
Cdd:smart01083   1 SWHPGNKKNQKRVWKAEQKAEEEKKKI 27

Pre-mRNA splicing factor; This domain family is found in eukaryotes, and is approximately 100 amino acids in length. The family is found in association with pfam10197. There is a single completely conserved residue Y that may be functionally important. Cwc25 has been identified to associate with pre-mRNA splicing factor Cef1/Ntc85, a component of the Prp19-associated complex (NTC) involved in spliceosome activation. Cwc25 is neither tightly associated with NTC nor required for spliceosome activation, but is required for the first catalytic reaction.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 262072988   67 EEKLDWMYQGPGGMVNR--DEYLLGRP-IDKYVFEK---MEEREAGCSSETGLLPGSIFAPSGANSLLDMASKIREDPLF 140
Cdd:pfam12542   1 LERLDWMYDGPSGGVEEemEEYLLGKKrVDKLLEGGddaEKKKAAKASFSAASVPGALFLEKAINSARDTARKLREDPLL 80

                          90
                  ....*....|....*..
gi 262072988  141 IIRKKEEEKKREVLNNP 157
Cdd:pfam12542  81 AIKKQEQEARQALLNNP 97

N-terminal domain of CBF1 interacting co-repressor CIR; This is a 45 residue conserved region at the N-terminal end of a family of proteins referred to as CIRs (CBF1-interacting co-repressors). CBF1 (centromere-binding factor 1) acts as a transcription factor that causes repression by binding specifically to GTGGGAA motifs in responsive promoters, and it requires CIR as a co-repressor. CIR binds to histone deacetylase and to SAP30 and serves as a linker between CBF1 and the histone deacetylase complex.

                           10        20
                   ....*....|....*....|....*..
gi 262072988    11 SWHPQTLRNVEKVWKAEQKHEAERKKI 37
Cdd:smart01083   1 SWHPGNKKNQKRVWKAEQKAEEEKKKI 27