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Conserved domains on  [gi|13385646|ref|NP_080412|]
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uncharacterized protein LOC67410 [Mus musculus]

Protein Classification

Ras-GEF domain-containing protein( domain architecture ID 10153433)

Ras guanine nucleotide exchange factor (Ras-GEF) domain-containing protein activates Ras-like small GTPases by mediating the replacement of GDP with GTP

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
REM cd06224
Guanine nucleotide exchange factor for Ras-like GTPases; N-terminal domain (RasGef_N), also ...
 89-177 1.65e-10

Guanine nucleotide exchange factor for Ras-like GTPases; N-terminal domain (RasGef_N), also called REM domain (Ras exchanger motif). This domain is common in nucleotide exchange factors for Ras-like small GTPases and is typically found immediately N-terminal to the RasGef (Cdc25-like) domain. REM contacts the GTPase and is assumed to participate in the catalytic activity of the exchange factor. Proteins with the REM domain include Sos1 and Sos2, which relay signals from tyrosine-kinase mediated signalling to Ras, RasGRP1-4, RasGRF1,2, CNrasGEF, and RAP-specific nucleotide exchange factors, to name a few.


:

Pssm-ID: 100121  Cd Length: 122  Bit Score: 57.81  E-value: 1.65e-10
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 13385646  89 ITIFLGVYRTYASTWEVLDLLMRTY--------ASFRPDCIKDQQTKRAIFRFLFGWFKKYPQDFYESPDLavVRQFIDY 160
Cdd:cd06224  21 VSTFLLTYRSFTTPTELLEKLIERYeiappenlEYNDWDKKKSKPIRLRVLNVLRTWVENYPYDFFDDEEL--LELLEEF 98

                        90
                ....*....|....*..
gi 13385646 161 VKRNVPSSDVDRARELL 177
Cdd:cd06224  99 LNRLVQEGALLQELKKL 115
 
Name Accession Description Interval E-value
REM cd06224
Guanine nucleotide exchange factor for Ras-like GTPases; N-terminal domain (RasGef_N), also ...
 89-177 1.65e-10

Guanine nucleotide exchange factor for Ras-like GTPases; N-terminal domain (RasGef_N), also called REM domain (Ras exchanger motif). This domain is common in nucleotide exchange factors for Ras-like small GTPases and is typically found immediately N-terminal to the RasGef (Cdc25-like) domain. REM contacts the GTPase and is assumed to participate in the catalytic activity of the exchange factor. Proteins with the REM domain include Sos1 and Sos2, which relay signals from tyrosine-kinase mediated signalling to Ras, RasGRP1-4, RasGRF1,2, CNrasGEF, and RAP-specific nucleotide exchange factors, to name a few.


Pssm-ID: 100121  Cd Length: 122  Bit Score: 57.81  E-value: 1.65e-10
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 13385646  89 ITIFLGVYRTYASTWEVLDLLMRTY--------ASFRPDCIKDQQTKRAIFRFLFGWFKKYPQDFYESPDLavVRQFIDY 160
Cdd:cd06224  21 VSTFLLTYRSFTTPTELLEKLIERYeiappenlEYNDWDKKKSKPIRLRVLNVLRTWVENYPYDFFDDEEL--LELLEEF 98

                        90
                ....*....|....*..
gi 13385646 161 VKRNVPSSDVDRARELL 177
Cdd:cd06224  99 LNRLVQEGALLQELKKL 115
RasGEFN smart00229
Guanine nucleotide exchange factor for Ras-like GTPases; N-terminal motif; A subset of guanine ...
 69-177 8.57e-10

Guanine nucleotide exchange factor for Ras-like GTPases; N-terminal motif; A subset of guanine nucleotide exchange factor for Ras-like small GTPases appear to possess this domain N-terminal to the RasGef (Cdc25-like) domain. The recent crystal structureof Sos shows that this domain is alpha-helical and plays a "purely structural role" (Nature 394, 337-343).


Pssm-ID: 214571  Cd Length: 127  Bit Score: 55.80  E-value: 8.57e-10
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 13385646     69 YVVQMIHYIPAAIQKRDHICITIFLGVYRTYASTWEVLDLLMRTYASFRP-----DCIKDQQTKRAIFRFLFGWFKKYPQ 143
Cdd:smart00229   9 TLEALIEHLTEAFDKADPSFVETFLLTYRSFITTQELLQLLLYRYNAIPPeswveEKVNPRRVKNRVLNILRTWVENYWE 88

                           90       100       110
                   ....*....|....*....|....*....|....*
gi 13385646    144 DFYESP-DLAVVRQFIDYVKRNVPSSDVDRARELL 177
Cdd:smart00229  89 DFEDDPkLISFLLEFLELVDDEKYPGLVTSLLNLL 123
RasGEF_N pfam00618
RasGEF N-terminal motif; A subset of guanine nucleotide exchange factor for Ras-like small ...
 89-151 1.65e-06

RasGEF N-terminal motif; A subset of guanine nucleotide exchange factor for Ras-like small GTPases appear to possess this motif/domain N-terminal to the RasGef (Cdc25-like) domain.


Pssm-ID: 459873  Cd Length: 104  Bit Score: 45.76  E-value: 1.65e-06
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 13385646    89 ITIFLGVYRTYASTWEVLDLLMRTYASFRP-DCIKDQQ--------TKRAIFRFLFGWFKKYPQDFYESPDL 151
Cdd:pfam00618  26 LSTFLLTYRSFTTPAELLELLIERYNIPPPlDLSSDSYwiskktlpIRIRVLSVLRHWVENYFSDFNDDPVL 97
 
Name Accession Description Interval E-value
REM cd06224
Guanine nucleotide exchange factor for Ras-like GTPases; N-terminal domain (RasGef_N), also ...
 89-177 1.65e-10

Guanine nucleotide exchange factor for Ras-like GTPases; N-terminal domain (RasGef_N), also called REM domain (Ras exchanger motif). This domain is common in nucleotide exchange factors for Ras-like small GTPases and is typically found immediately N-terminal to the RasGef (Cdc25-like) domain. REM contacts the GTPase and is assumed to participate in the catalytic activity of the exchange factor. Proteins with the REM domain include Sos1 and Sos2, which relay signals from tyrosine-kinase mediated signalling to Ras, RasGRP1-4, RasGRF1,2, CNrasGEF, and RAP-specific nucleotide exchange factors, to name a few.


Pssm-ID: 100121  Cd Length: 122  Bit Score: 57.81  E-value: 1.65e-10
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 13385646  89 ITIFLGVYRTYASTWEVLDLLMRTY--------ASFRPDCIKDQQTKRAIFRFLFGWFKKYPQDFYESPDLavVRQFIDY 160
Cdd:cd06224  21 VSTFLLTYRSFTTPTELLEKLIERYeiappenlEYNDWDKKKSKPIRLRVLNVLRTWVENYPYDFFDDEEL--LELLEEF 98

                        90
                ....*....|....*..
gi 13385646 161 VKRNVPSSDVDRARELL 177
Cdd:cd06224  99 LNRLVQEGALLQELKKL 115
RasGEFN smart00229
Guanine nucleotide exchange factor for Ras-like GTPases; N-terminal motif; A subset of guanine ...
 69-177 8.57e-10

Guanine nucleotide exchange factor for Ras-like GTPases; N-terminal motif; A subset of guanine nucleotide exchange factor for Ras-like small GTPases appear to possess this domain N-terminal to the RasGef (Cdc25-like) domain. The recent crystal structureof Sos shows that this domain is alpha-helical and plays a "purely structural role" (Nature 394, 337-343).


Pssm-ID: 214571  Cd Length: 127  Bit Score: 55.80  E-value: 8.57e-10
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 13385646     69 YVVQMIHYIPAAIQKRDHICITIFLGVYRTYASTWEVLDLLMRTYASFRP-----DCIKDQQTKRAIFRFLFGWFKKYPQ 143
Cdd:smart00229   9 TLEALIEHLTEAFDKADPSFVETFLLTYRSFITTQELLQLLLYRYNAIPPeswveEKVNPRRVKNRVLNILRTWVENYWE 88

                           90       100       110
                   ....*....|....*....|....*....|....*
gi 13385646    144 DFYESP-DLAVVRQFIDYVKRNVPSSDVDRARELL 177
Cdd:smart00229  89 DFEDDPkLISFLLEFLELVDDEKYPGLVTSLLNLL 123
RasGEF_N pfam00618
RasGEF N-terminal motif; A subset of guanine nucleotide exchange factor for Ras-like small ...
 89-151 1.65e-06

RasGEF N-terminal motif; A subset of guanine nucleotide exchange factor for Ras-like small GTPases appear to possess this motif/domain N-terminal to the RasGef (Cdc25-like) domain.


Pssm-ID: 459873  Cd Length: 104  Bit Score: 45.76  E-value: 1.65e-06
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 13385646    89 ITIFLGVYRTYASTWEVLDLLMRTYASFRP-DCIKDQQ--------TKRAIFRFLFGWFKKYPQDFYESPDL 151
Cdd:pfam00618  26 LSTFLLTYRSFTTPAELLELLIERYNIPPPlDLSSDSYwiskktlpIRIRVLSVLRHWVENYFSDFNDDPVL 97
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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