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Conserved domains on  [gi|6323928|ref|NP_013999|]
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fatty acid alpha-hydroxylase [Saccharomyces cerevisiae S288C]

Protein Classification

fatty acid 2-hydroxylase( domain architecture ID 10445814)

fatty acid 2-hydroxylase is an integral membrane involved in processes such as cholesterol biosynthesis or biosynthesis of plant cuticular wax. It catalyzes the hydroxylation of free fatty acids at the C-2 position to produce 2-hydroxy fatty acids, which are building blocks of sphingolipids and glycosphingolipids, for example, and contains an N-terminal cytochrome b5-like heme/steroid binding domain

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
FA_hydroxylase super family cl01132
Fatty acid hydroxylase; This entry includes fatty acid and carotene hydroxylases and sterol ...
 149-380 3.53e-73

Fatty acid hydroxylase; This entry includes fatty acid and carotene hydroxylases and sterol desaturases. Beta-carotene hydroxylase is involved in zeaxanthin synthesis by hydroxylating beta-carotene, but the enzyme may be involved in other pathways. This family includes C-5 sterol desaturase and C-4 sterol methyl oxidase. Members of this family are involved in cholesterol biosynthesis and biosynthesis a plant cuticular wax. These enzymes contain two copies of a HXHH motif which coordinate two irons at the catalytic centre. Members of this family are ER integral membrane proteins that share a novel mushroom-shaped fold consisting of four transmembrane (TM1-TM4) helices that anchor them to the membrane capped by a cytosolic domain containing the unique histidine- coordinating di metal centre.


The actual alignment was detected with superfamily member PLN02434:

Pssm-ID: 412761 [Multi-domain]  Cd Length: 237  Bit Score: 227.99  E-value: 3.53e-73
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6323928   149 DLNRPLLMQI--LRSDfkkdfYVDQIHRPRhYGKGSAPLFGN-FLEPLTKTAWWVVPVAWLPVVVYHMGVALKNMNQLFA 225
Cdd:PLN02434   9 DLNKPLVFQVghLGED-----YEEWVHQPI-VSKEGPRFFESdVLEFLTRTVWWAVPLIWLPVVCWCLVKSVRMGLPLSA 82

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6323928   226 C-FLFCVGVFVWTLIEYGLHRFLFHFDdwlPESNIAFATHFLLHGCHHYLPMDKYRLVMPPTLFVILCAPFYKLVFALLP 304
Cdd:PLN02434  83 VvLMVAFGVFIWTLLEYILHRFLFHIK---TKSYWGNTAHYLLHGCHHKHPMDGLRLVFPPAATAILCVPFWNLIALFAT 159

                        170       180       190       200       210       220       230
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 6323928   305 LYWAYAGFAGGLFGYVCYDECHFFLHH-SKLPPFMRKLKKYHLEHHYKNYQLGFGVTSWFWDEVFGTYLGPDAPLSK 380
Cdd:PLN02434 160 PATAPALFGGGLLGYVMYDCTHYFLHHgQPSTDVLRNLKKYHLNHHFRDQDKGFGITSSLWDRVFGTLPPSKAAKKK 236
Cyt-b5 pfam00173
Cytochrome b5-like Heme/Steroid binding domain; This family includes heme binding domains from ...
 13-90 5.90e-24

Cytochrome b5-like Heme/Steroid binding domain; This family includes heme binding domains from a diverse range of proteins. This family also includes proteins that bind to steroids. The family includes progesterone receptors. Many members of this subfamily are membrane anchored by an N-terminal transmembrane alpha helix. This family also includes a domain in some chitin synthases. There is no known ligand for this domain in the chitin synthases.


:

Pssm-ID: 459698 [Multi-domain]  Cd Length: 74  Bit Score: 93.84  E-value: 5.90e-24
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 6323928     13 SKKTVQEHNTANDCWVTYqNRKIYDVTRFLSEHPGGDESILDYAGKDITEIMKDsdvHEHSDSAYEILEDEYLIGYLA 90
Cdd:pfam00173   1 TLEELSKHNGDGDCWVAI-NGKVYDVTKFLKEHPGGEDVILSAAGKDATDAFEA---IGHSEDAAEKLLKKYRIGELA 74
 
Name Accession Description Interval E-value
PLN02434 PLN02434
fatty acid hydroxylase
 149-380 3.53e-73

fatty acid hydroxylase


Pssm-ID: 178053 [Multi-domain]  Cd Length: 237  Bit Score: 227.99  E-value: 3.53e-73
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6323928   149 DLNRPLLMQI--LRSDfkkdfYVDQIHRPRhYGKGSAPLFGN-FLEPLTKTAWWVVPVAWLPVVVYHMGVALKNMNQLFA 225
Cdd:PLN02434   9 DLNKPLVFQVghLGED-----YEEWVHQPI-VSKEGPRFFESdVLEFLTRTVWWAVPLIWLPVVCWCLVKSVRMGLPLSA 82

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6323928   226 C-FLFCVGVFVWTLIEYGLHRFLFHFDdwlPESNIAFATHFLLHGCHHYLPMDKYRLVMPPTLFVILCAPFYKLVFALLP 304
Cdd:PLN02434  83 VvLMVAFGVFIWTLLEYILHRFLFHIK---TKSYWGNTAHYLLHGCHHKHPMDGLRLVFPPAATAILCVPFWNLIALFAT 159

                        170       180       190       200       210       220       230
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 6323928   305 LYWAYAGFAGGLFGYVCYDECHFFLHH-SKLPPFMRKLKKYHLEHHYKNYQLGFGVTSWFWDEVFGTYLGPDAPLSK 380
Cdd:PLN02434 160 PATAPALFGGGLLGYVMYDCTHYFLHHgQPSTDVLRNLKKYHLNHHFRDQDKGFGITSSLWDRVFGTLPPSKAAKKK 236
Cyt-b5 pfam00173
Cytochrome b5-like Heme/Steroid binding domain; This family includes heme binding domains from ...
 13-90 5.90e-24

Cytochrome b5-like Heme/Steroid binding domain; This family includes heme binding domains from a diverse range of proteins. This family also includes proteins that bind to steroids. The family includes progesterone receptors. Many members of this subfamily are membrane anchored by an N-terminal transmembrane alpha helix. This family also includes a domain in some chitin synthases. There is no known ligand for this domain in the chitin synthases.


Pssm-ID: 459698 [Multi-domain]  Cd Length: 74  Bit Score: 93.84  E-value: 5.90e-24
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 6323928     13 SKKTVQEHNTANDCWVTYqNRKIYDVTRFLSEHPGGDESILDYAGKDITEIMKDsdvHEHSDSAYEILEDEYLIGYLA 90
Cdd:pfam00173   1 TLEELSKHNGDGDCWVAI-NGKVYDVTKFLKEHPGGEDVILSAAGKDATDAFEA---IGHSEDAAEKLLKKYRIGELA 74
CYB5 COG5274
Cytochrome b involved in lipid metabolism [Energy production and conversion, Lipid transport ...
 17-91 2.01e-19

Cytochrome b involved in lipid metabolism [Energy production and conversion, Lipid transport and metabolism];


Pssm-ID: 444085 [Multi-domain]  Cd Length: 93  Bit Score: 82.39  E-value: 2.01e-19
                        10        20        30        40        50        60        70
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 6323928   17 VQEHNTANDCWVTYqNRKIYDVTRFLSEHPGGDESILDYAGKDITEIMKdsDVHEHSDSAYEILEDeYLIGYLAT 91
Cdd:COG5274  23 VATHNTLSDCWMAI-DGNVYDLTEYIPKHPGGEAVILRWCGKDATEAFN--TKHPHSPKAERLLES-YRIGRLAQ 93
FA_hydroxylase pfam04116
Fatty acid hydroxylase; This entry includes fatty acid and carotene hydroxylases and sterol ...
 229-370 1.80e-18

Fatty acid hydroxylase; This entry includes fatty acid and carotene hydroxylases and sterol desaturases. Beta-carotene hydroxylase is involved in zeaxanthin synthesis by hydroxylating beta-carotene, but the enzyme may be involved in other pathways. This family includes C-5 sterol desaturase and C-4 sterol methyl oxidase. Members of this family are involved in cholesterol biosynthesis and biosynthesis a plant cuticular wax. These enzymes contain two copies of a HXHH motif which coordinate two irons at the catalytic centre. Members of this family are ER integral membrane proteins that share a novel mushroom-shaped fold consisting of four transmembrane (TM1-TM4) helices that anchor them to the membrane capped by a cytosolic domain containing the unique histidine- coordinating di metal centre.


Pssm-ID: 397991  Cd Length: 134  Bit Score: 80.94  E-value: 1.80e-18
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6323928    229 FCVGVFVWTLIEYGLHRfLFHFDDWLpesniafatHFLLHGCHH-YLPMDKYRLVMPPTLFVILCAPFYKLVFALLPLYW 307
Cdd:pfam04116   1 VLLGLLLFDFLFYWVHR-LLHRLPWL---------WRRFHKVHHsSEAPNALTALRFHPLEALLFALLVLLPLLLLGLPL 70

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 6323928    308 aYAGFAGGLFGYVCYDECHFFLHHSKLPP--FMRKLKKYHLEHHYKNYQLGFGVTSWFWDEVFGT 370
Cdd:pfam04116  71 -LAFLLGLLLGTLWYLFIHSGLLFPLPWLlkRLLGTPRFHRLHHSKNEEYNFGVTFPLWDRLFGT 134
PLN02252 PLN02252
nitrate reductase [NADPH]
 3-96 4.74e-17

nitrate reductase [NADPH]


Pssm-ID: 215141 [Multi-domain]  Cd Length: 888  Bit Score: 83.19  E-value: 4.74e-17
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6323928     3 TNTSKTLELFSKKTVQEHNTANDCWVTYQNrKIYDVTRFLSEHPGGDESILDYAGKDITEimkDSDVhEHSDSAYEILED 82
Cdd:PLN02252 511 MNTNTGSKQYTMSEVRKHNSEDSCWIVVHG-HVYDCTRFLKDHPGGADSILINAGTDCTE---EFDA-IHSDKAKKMLED 585

                         90
                 ....*....|....
gi 6323928    83 eYLIGYLATDEEAA 96
Cdd:PLN02252 586 -YRIGELVTTGAAA 598
ERG3 COG3000
Sterol desaturase/sphingolipid hydroxylase, fatty acid hydroxylase superfamily [Lipid ...
 229-377 4.26e-04

Sterol desaturase/sphingolipid hydroxylase, fatty acid hydroxylase superfamily [Lipid transport and metabolism];


Pssm-ID: 442238  Cd Length: 158  Bit Score: 40.30  E-value: 4.26e-04
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6323928  229 FCVGVFVWTLIEYGLHRfLFHFDDWLpesniafathFLLHGCHHylpMDKYR-----LVMPPtLFVILCAPFYKLVFALL 303
Cdd:COG3000   2 FLLAFLLLDFVYYWLHR-LSHRVPLL----------WRFHAVHH---SSERMnlltaLRFHP-LEILLSALLFLLPLALL 66

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6323928  304 PLYWaYAGFAGGLFGYVcydecHFFLHHSKL-PPFMRKLKK------YHLEHHYKNYQLG---FGVTSWFWDEVFGTYLG 373
Cdd:COG3000  67 GFPP-EAVLLYLALNLL-----YQFFNHSNIrLPLDGPLEYvfvtpsHHRVHHSRNPEYLdknYGGILSIWDRLFGTYRP 140


                ....
gi 6323928  374 PDAP 377
Cdd:COG3000 141 EERE 144
 
Name Accession Description Interval E-value
PLN02434 PLN02434
fatty acid hydroxylase
 149-380 3.53e-73

fatty acid hydroxylase


Pssm-ID: 178053 [Multi-domain]  Cd Length: 237  Bit Score: 227.99  E-value: 3.53e-73
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6323928   149 DLNRPLLMQI--LRSDfkkdfYVDQIHRPRhYGKGSAPLFGN-FLEPLTKTAWWVVPVAWLPVVVYHMGVALKNMNQLFA 225
Cdd:PLN02434   9 DLNKPLVFQVghLGED-----YEEWVHQPI-VSKEGPRFFESdVLEFLTRTVWWAVPLIWLPVVCWCLVKSVRMGLPLSA 82

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6323928   226 C-FLFCVGVFVWTLIEYGLHRFLFHFDdwlPESNIAFATHFLLHGCHHYLPMDKYRLVMPPTLFVILCAPFYKLVFALLP 304
Cdd:PLN02434  83 VvLMVAFGVFIWTLLEYILHRFLFHIK---TKSYWGNTAHYLLHGCHHKHPMDGLRLVFPPAATAILCVPFWNLIALFAT 159

                        170       180       190       200       210       220       230
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 6323928   305 LYWAYAGFAGGLFGYVCYDECHFFLHH-SKLPPFMRKLKKYHLEHHYKNYQLGFGVTSWFWDEVFGTYLGPDAPLSK 380
Cdd:PLN02434 160 PATAPALFGGGLLGYVMYDCTHYFLHHgQPSTDVLRNLKKYHLNHHFRDQDKGFGITSSLWDRVFGTLPPSKAAKKK 236
Cyt-b5 pfam00173
Cytochrome b5-like Heme/Steroid binding domain; This family includes heme binding domains from ...
 13-90 5.90e-24

Cytochrome b5-like Heme/Steroid binding domain; This family includes heme binding domains from a diverse range of proteins. This family also includes proteins that bind to steroids. The family includes progesterone receptors. Many members of this subfamily are membrane anchored by an N-terminal transmembrane alpha helix. This family also includes a domain in some chitin synthases. There is no known ligand for this domain in the chitin synthases.


Pssm-ID: 459698 [Multi-domain]  Cd Length: 74  Bit Score: 93.84  E-value: 5.90e-24
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 6323928     13 SKKTVQEHNTANDCWVTYqNRKIYDVTRFLSEHPGGDESILDYAGKDITEIMKDsdvHEHSDSAYEILEDEYLIGYLA 90
Cdd:pfam00173   1 TLEELSKHNGDGDCWVAI-NGKVYDVTKFLKEHPGGEDVILSAAGKDATDAFEA---IGHSEDAAEKLLKKYRIGELA 74
CYB5 COG5274
Cytochrome b involved in lipid metabolism [Energy production and conversion, Lipid transport ...
 17-91 2.01e-19

Cytochrome b involved in lipid metabolism [Energy production and conversion, Lipid transport and metabolism];


Pssm-ID: 444085 [Multi-domain]  Cd Length: 93  Bit Score: 82.39  E-value: 2.01e-19
                        10        20        30        40        50        60        70
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 6323928   17 VQEHNTANDCWVTYqNRKIYDVTRFLSEHPGGDESILDYAGKDITEIMKdsDVHEHSDSAYEILEDeYLIGYLAT 91
Cdd:COG5274  23 VATHNTLSDCWMAI-DGNVYDLTEYIPKHPGGEAVILRWCGKDATEAFN--TKHPHSPKAERLLES-YRIGRLAQ 93
FA_hydroxylase pfam04116
Fatty acid hydroxylase; This entry includes fatty acid and carotene hydroxylases and sterol ...
 229-370 1.80e-18

Fatty acid hydroxylase; This entry includes fatty acid and carotene hydroxylases and sterol desaturases. Beta-carotene hydroxylase is involved in zeaxanthin synthesis by hydroxylating beta-carotene, but the enzyme may be involved in other pathways. This family includes C-5 sterol desaturase and C-4 sterol methyl oxidase. Members of this family are involved in cholesterol biosynthesis and biosynthesis a plant cuticular wax. These enzymes contain two copies of a HXHH motif which coordinate two irons at the catalytic centre. Members of this family are ER integral membrane proteins that share a novel mushroom-shaped fold consisting of four transmembrane (TM1-TM4) helices that anchor them to the membrane capped by a cytosolic domain containing the unique histidine- coordinating di metal centre.


Pssm-ID: 397991  Cd Length: 134  Bit Score: 80.94  E-value: 1.80e-18
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6323928    229 FCVGVFVWTLIEYGLHRfLFHFDDWLpesniafatHFLLHGCHH-YLPMDKYRLVMPPTLFVILCAPFYKLVFALLPLYW 307
Cdd:pfam04116   1 VLLGLLLFDFLFYWVHR-LLHRLPWL---------WRRFHKVHHsSEAPNALTALRFHPLEALLFALLVLLPLLLLGLPL 70

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 6323928    308 aYAGFAGGLFGYVCYDECHFFLHHSKLPP--FMRKLKKYHLEHHYKNYQLGFGVTSWFWDEVFGT 370
Cdd:pfam04116  71 -LAFLLGLLLGTLWYLFIHSGLLFPLPWLlkRLLGTPRFHRLHHSKNEEYNFGVTFPLWDRLFGT 134
PLN02252 PLN02252
nitrate reductase [NADPH]
 3-96 4.74e-17

nitrate reductase [NADPH]


Pssm-ID: 215141 [Multi-domain]  Cd Length: 888  Bit Score: 83.19  E-value: 4.74e-17
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6323928     3 TNTSKTLELFSKKTVQEHNTANDCWVTYQNrKIYDVTRFLSEHPGGDESILDYAGKDITEimkDSDVhEHSDSAYEILED 82
Cdd:PLN02252 511 MNTNTGSKQYTMSEVRKHNSEDSCWIVVHG-HVYDCTRFLKDHPGGADSILINAGTDCTE---EFDA-IHSDKAKKMLED 585

                         90
                 ....*....|....
gi 6323928    83 eYLIGYLATDEEAA 96
Cdd:PLN02252 586 -YRIGELVTTGAAA 598
PLN03198 PLN03198
delta6-acyl-lipid desaturase; Provisional
 17-101 9.15e-09

delta6-acyl-lipid desaturase; Provisional


Pssm-ID: 178739 [Multi-domain]  Cd Length: 526  Bit Score: 57.01  E-value: 9.15e-09
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6323928    17 VQEHNTANDCWVTYQNrKIYDVTRFLSEHPGGdESILDYAGKDITEIMKDSdvheHSDSAYEILEDEYlIGYLATDEEAA 96
Cdd:PLN03198 111 VAAHNKPNDCWIVIKN-KVYDVSDFAAEHPGG-SVISTYFGRDGTDAFSSF----HAASTWKILQDFY-IGDVDNVEPTP 183


                 ....*
gi 6323928    97 RLLTN 101
Cdd:PLN03198 184 ELLKD 188
PLN03199 PLN03199
delta6-acyl-lipid desaturase-like protein; Provisional
 2-63 7.04e-05

delta6-acyl-lipid desaturase-like protein; Provisional


Pssm-ID: 178740 [Multi-domain]  Cd Length: 485  Bit Score: 44.64  E-value: 7.04e-05
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 6323928     2 STNTSKTLELFSKKTVQE---HNTANDCWVTYQNrKIYDVTRFlSEHPGGdESILDYAGKDITEI 63
Cdd:PLN03199  13 RSAALKLAEKPQKISWQEvkkHASPDDAWIIHQN-KVYDVSNW-HDHPGG-AVIFTHAGDDMTDI 74
ERG3 COG3000
Sterol desaturase/sphingolipid hydroxylase, fatty acid hydroxylase superfamily [Lipid ...
 229-377 4.26e-04

Sterol desaturase/sphingolipid hydroxylase, fatty acid hydroxylase superfamily [Lipid transport and metabolism];


Pssm-ID: 442238  Cd Length: 158  Bit Score: 40.30  E-value: 4.26e-04
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6323928  229 FCVGVFVWTLIEYGLHRfLFHFDDWLpesniafathFLLHGCHHylpMDKYR-----LVMPPtLFVILCAPFYKLVFALL 303
Cdd:COG3000   2 FLLAFLLLDFVYYWLHR-LSHRVPLL----------WRFHAVHH---SSERMnlltaLRFHP-LEILLSALLFLLPLALL 66

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6323928  304 PLYWaYAGFAGGLFGYVcydecHFFLHHSKL-PPFMRKLKK------YHLEHHYKNYQLG---FGVTSWFWDEVFGTYLG 373
Cdd:COG3000  67 GFPP-EAVLLYLALNLL-----YQFFNHSNIrLPLDGPLEYvfvtpsHHRVHHSRNPEYLdknYGGILSIWDRLFGTYRP 140


                ....
gi 6323928  374 PDAP 377
Cdd:COG3000 141 EERE 144
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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