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Conserved domains on  [gi|6320433|ref|NP_010513|]
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chromatin-silencing protein SIR4 [Saccharomyces cerevisiae S288C]

Protein Classification

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
Sir4p-SID_like cd13746
The SID domain of Saccharomyces cerevisiae silent information regulator 4, a Sir2p interaction ...
 801-893 2.06e-36

The SID domain of Saccharomyces cerevisiae silent information regulator 4, a Sir2p interaction domain; and related domains; Saccharomyces cerevisiae Sir2p, Sir3p, and Sir4p form a heterotrimeric complex which binds chromatin and represses transcription at the homothallic mating type (HM) loci and at subtelomeric regions. This domain model spans residues 742-893 of Sir4p. Sir4p forms a stable heterodimer with Sir2p, mediated by Sir4p residues included in this domain, and a pocket between Sir2p's catalytic domain and its non-conserved N-terminus. Sir4p also interacts with an array of additional factors, including Yku80p, a subunit of the telomeric Ku complex (Yku70p-Yku80p), which binds two sites within Sir4p, one at the N-terminus and one in the C-terminal residues, 731-1358. Other interaction factors include Esc1p (Establishes silent chromatin 1) which binds the Sir4p PAD domain (partitioning and anchoring domain, residues 950-1262), and Sir3p, Yku70p, and Rap1p (Repressor Activator Protein) which bind in its C-terminal coiled-coil (residues 1257-1358). Other Sir4p interacting factors include the Ty5 retrotransposon. Additional roles for Sir4p include roles in DNA repair, and in aging. A SIR4 mutant having a truncated Sir4p lacking a C-terminal coiled-coil domain, has an extended mean life span; deletion of the SIR4 gene leads to a decreased mean life span.


:

Pssm-ID: 259839  Cd Length: 115  Bit Score: 133.56  E-value: 2.06e-36
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6320433   801 DPVLDWRKSDRELTKNILWRIADKTTYDKETITDLIEQGIPKHSYLSGNPLTSVTNDICSVENYETSSAFFYQQVHKKDR 880
Cdd:cd13746   23 DPELERKKIDRELEKYVNKKLADKKTYDKSTIIDLIESGIATHTPLSSDPKVSVTNNICSVNNYESSHAFYYQQLHKRDR 102

                         90
                 ....*....|...
gi 6320433   881 LQYLPLYAVSTFE 893
Cdd:cd13746  103 LEYLPLYEPLTPE 115
Sir4_CC_C cd22863
C-terminal coiled coil domain found in Saccharomyces cerevisiae silent information regulator 4 ...
 1272-1350 6.40e-31

C-terminal coiled coil domain found in Saccharomyces cerevisiae silent information regulator 4 (Sir4p) and similar proteins; Sir4p is a regulatory protein that plays important roles in silent chromatin at telomeric and silent mating type loci. It physically interacts with another two silent information regulators, Sir2p and Sir3p, to form a complex that may regulate chromatin structure. The C-terminus of Sir4p is critical for its functions in vivo. This model corresponds to the C-terminal coiled coil domain of Sir4. Coiled coil dimerization is essential for telomeric and mating type silencing in Saccharomyces cerevisiae.


:

Pssm-ID: 439379 [Multi-domain]  Cd Length: 79  Bit Score: 116.48  E-value: 6.40e-31
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 6320433  1272 SFVDIVLSKAASALDEKEKQLAVANEIIRSLSDEVMRNEIRITSLQGDLTFTKKCLENARSQISEKDAKINKLMEKDFQ 1350
Cdd:cd22863    1 SFVDILLEDAASALDEKDKQLAAANKIILALSREVMRNEIQITSLTGDLTFTKKELENQRSQISEKDAKINKQEEKMFQ 79
PTZ00449 super family cl33186
104 kDa microneme/rhoptry antigen; Provisional
 2-106 1.38e-03

104 kDa microneme/rhoptry antigen; Provisional


The actual alignment was detected with superfamily member PTZ00449:

Pssm-ID: 185628 [Multi-domain]  Cd Length: 943  Bit Score: 43.14  E-value: 1.38e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6320433      2 PNDNKTPNRSSTPKFTKKPVTPND----KIPEREEKSnevKTPKIPLFTFAKSKNYSRPSTAIHTSPHQPSDVK-PTSHK 76
Cdd:PTZ00449  582 PKDPKHPKDPEEPKKPKRPRSAQRptrpKSPKLPELL---DIPKSPKRPESPKSPKRPPPPQRPSSPERPEGPKiIKSPK 658

                          90       100       110
                  ....*....|....*....|....*....|
gi 6320433     77 QLQQPKsSPLKKNNYNSFPHSNLEKISNSK 106
Cdd:PTZ00449  659 PPKSPK-PPFDPKFKEKFYDDYLDAAAKSK 687
 
Name Accession Description Interval E-value
Sir4p-SID_like cd13746
The SID domain of Saccharomyces cerevisiae silent information regulator 4, a Sir2p interaction ...
 801-893 2.06e-36

The SID domain of Saccharomyces cerevisiae silent information regulator 4, a Sir2p interaction domain; and related domains; Saccharomyces cerevisiae Sir2p, Sir3p, and Sir4p form a heterotrimeric complex which binds chromatin and represses transcription at the homothallic mating type (HM) loci and at subtelomeric regions. This domain model spans residues 742-893 of Sir4p. Sir4p forms a stable heterodimer with Sir2p, mediated by Sir4p residues included in this domain, and a pocket between Sir2p's catalytic domain and its non-conserved N-terminus. Sir4p also interacts with an array of additional factors, including Yku80p, a subunit of the telomeric Ku complex (Yku70p-Yku80p), which binds two sites within Sir4p, one at the N-terminus and one in the C-terminal residues, 731-1358. Other interaction factors include Esc1p (Establishes silent chromatin 1) which binds the Sir4p PAD domain (partitioning and anchoring domain, residues 950-1262), and Sir3p, Yku70p, and Rap1p (Repressor Activator Protein) which bind in its C-terminal coiled-coil (residues 1257-1358). Other Sir4p interacting factors include the Ty5 retrotransposon. Additional roles for Sir4p include roles in DNA repair, and in aging. A SIR4 mutant having a truncated Sir4p lacking a C-terminal coiled-coil domain, has an extended mean life span; deletion of the SIR4 gene leads to a decreased mean life span.


Pssm-ID: 259839  Cd Length: 115  Bit Score: 133.56  E-value: 2.06e-36
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6320433   801 DPVLDWRKSDRELTKNILWRIADKTTYDKETITDLIEQGIPKHSYLSGNPLTSVTNDICSVENYETSSAFFYQQVHKKDR 880
Cdd:cd13746   23 DPELERKKIDRELEKYVNKKLADKKTYDKSTIIDLIESGIATHTPLSSDPKVSVTNNICSVNNYESSHAFYYQQLHKRDR 102

                         90
                 ....*....|...
gi 6320433   881 LQYLPLYAVSTFE 893
Cdd:cd13746  103 LEYLPLYEPLTPE 115
Sir4_CC_C cd22863
C-terminal coiled coil domain found in Saccharomyces cerevisiae silent information regulator 4 ...
 1272-1350 6.40e-31

C-terminal coiled coil domain found in Saccharomyces cerevisiae silent information regulator 4 (Sir4p) and similar proteins; Sir4p is a regulatory protein that plays important roles in silent chromatin at telomeric and silent mating type loci. It physically interacts with another two silent information regulators, Sir2p and Sir3p, to form a complex that may regulate chromatin structure. The C-terminus of Sir4p is critical for its functions in vivo. This model corresponds to the C-terminal coiled coil domain of Sir4. Coiled coil dimerization is essential for telomeric and mating type silencing in Saccharomyces cerevisiae.


Pssm-ID: 439379 [Multi-domain]  Cd Length: 79  Bit Score: 116.48  E-value: 6.40e-31
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 6320433  1272 SFVDIVLSKAASALDEKEKQLAVANEIIRSLSDEVMRNEIRITSLQGDLTFTKKCLENARSQISEKDAKINKLMEKDFQ 1350
Cdd:cd22863    1 SFVDILLEDAASALDEKDKQLAAANKIILALSREVMRNEIQITSLTGDLTFTKKELENQRSQISEKDAKINKQEEKMFQ 79
SIR4_SID pfam16991
Sir4 SID domain; This is the Sir2 interaction domain (SID domain) of silent information ...
 746-886 3.53e-27

Sir4 SID domain; This is the Sir2 interaction domain (SID domain) of silent information regulator 4 (Sir4).


Pssm-ID: 407197  Cd Length: 159  Bit Score: 108.73  E-value: 3.53e-27
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6320433     746 KMELVYVS-DSDDSSSDNDSLTDLESLSSGESNEIKVTN--------------DLDTSAEKDQIqagkwFDPVLDWRKSD 810
Cdd:pfam16991    1 KMEIIYISdSDEESENDEASGTDEENSSSRSVDDGEISNyiknsstpltallvDSETSSEGSSI-----GDPETERKKYD 75

                           90       100       110       120       130       140       150
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 6320433     811 RELTKNILWRIADKTTYDKETITDLIEQGIPKHSYLSGNPLTSVTNDICSVENYETSSAFFYQQVHKKDRLQYLPL 886
Cdd:pfam16991   76 RQLAIRVNEKLAKKKAFSKSTISDLIESGIAPHTPLESDPYVSVTNNICSVRNYESSHAFHYQQLHKKDRLEYLPI 151
PTZ00449 PTZ00449
104 kDa microneme/rhoptry antigen; Provisional
 2-106 1.38e-03

104 kDa microneme/rhoptry antigen; Provisional


Pssm-ID: 185628 [Multi-domain]  Cd Length: 943  Bit Score: 43.14  E-value: 1.38e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6320433      2 PNDNKTPNRSSTPKFTKKPVTPND----KIPEREEKSnevKTPKIPLFTFAKSKNYSRPSTAIHTSPHQPSDVK-PTSHK 76
Cdd:PTZ00449  582 PKDPKHPKDPEEPKKPKRPRSAQRptrpKSPKLPELL---DIPKSPKRPESPKSPKRPPPPQRPSSPERPEGPKiIKSPK 658

                          90       100       110
                  ....*....|....*....|....*....|
gi 6320433     77 QLQQPKsSPLKKNNYNSFPHSNLEKISNSK 106
Cdd:PTZ00449  659 PPKSPK-PPFDPKFKEKFYDDYLDAAAKSK 687
CwlO1 COG3883
Uncharacterized N-terminal coiled-coil domain of peptidoglycan hydrolase CwlO [Function ...
 1275-1347 9.23e-03

Uncharacterized N-terminal coiled-coil domain of peptidoglycan hydrolase CwlO [Function unknown];


Pssm-ID: 443091 [Multi-domain]  Cd Length: 379  Bit Score: 40.20  E-value: 9.23e-03
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 6320433  1275 DIVLSKAASALDEKEKQLAVANEIIRSLSDEVMRNEIRITSLQGDLTFTKKCLENARSQISEKDAKINKLMEK 1347
Cdd:COG3883   15 DPQIQAKQKELSELQAELEAAQAELDALQAELEELNEEYNELQAELEALQAEIDKLQAEIAEAEAEIEERREE 87
 
Name Accession Description Interval E-value
Sir4p-SID_like cd13746
The SID domain of Saccharomyces cerevisiae silent information regulator 4, a Sir2p interaction ...
 801-893 2.06e-36

The SID domain of Saccharomyces cerevisiae silent information regulator 4, a Sir2p interaction domain; and related domains; Saccharomyces cerevisiae Sir2p, Sir3p, and Sir4p form a heterotrimeric complex which binds chromatin and represses transcription at the homothallic mating type (HM) loci and at subtelomeric regions. This domain model spans residues 742-893 of Sir4p. Sir4p forms a stable heterodimer with Sir2p, mediated by Sir4p residues included in this domain, and a pocket between Sir2p's catalytic domain and its non-conserved N-terminus. Sir4p also interacts with an array of additional factors, including Yku80p, a subunit of the telomeric Ku complex (Yku70p-Yku80p), which binds two sites within Sir4p, one at the N-terminus and one in the C-terminal residues, 731-1358. Other interaction factors include Esc1p (Establishes silent chromatin 1) which binds the Sir4p PAD domain (partitioning and anchoring domain, residues 950-1262), and Sir3p, Yku70p, and Rap1p (Repressor Activator Protein) which bind in its C-terminal coiled-coil (residues 1257-1358). Other Sir4p interacting factors include the Ty5 retrotransposon. Additional roles for Sir4p include roles in DNA repair, and in aging. A SIR4 mutant having a truncated Sir4p lacking a C-terminal coiled-coil domain, has an extended mean life span; deletion of the SIR4 gene leads to a decreased mean life span.


Pssm-ID: 259839  Cd Length: 115  Bit Score: 133.56  E-value: 2.06e-36
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6320433   801 DPVLDWRKSDRELTKNILWRIADKTTYDKETITDLIEQGIPKHSYLSGNPLTSVTNDICSVENYETSSAFFYQQVHKKDR 880
Cdd:cd13746   23 DPELERKKIDRELEKYVNKKLADKKTYDKSTIIDLIESGIATHTPLSSDPKVSVTNNICSVNNYESSHAFYYQQLHKRDR 102

                         90
                 ....*....|...
gi 6320433   881 LQYLPLYAVSTFE 893
Cdd:cd13746  103 LEYLPLYEPLTPE 115
Sir4_CC_C cd22863
C-terminal coiled coil domain found in Saccharomyces cerevisiae silent information regulator 4 ...
 1272-1350 6.40e-31

C-terminal coiled coil domain found in Saccharomyces cerevisiae silent information regulator 4 (Sir4p) and similar proteins; Sir4p is a regulatory protein that plays important roles in silent chromatin at telomeric and silent mating type loci. It physically interacts with another two silent information regulators, Sir2p and Sir3p, to form a complex that may regulate chromatin structure. The C-terminus of Sir4p is critical for its functions in vivo. This model corresponds to the C-terminal coiled coil domain of Sir4. Coiled coil dimerization is essential for telomeric and mating type silencing in Saccharomyces cerevisiae.


Pssm-ID: 439379 [Multi-domain]  Cd Length: 79  Bit Score: 116.48  E-value: 6.40e-31
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 6320433  1272 SFVDIVLSKAASALDEKEKQLAVANEIIRSLSDEVMRNEIRITSLQGDLTFTKKCLENARSQISEKDAKINKLMEKDFQ 1350
Cdd:cd22863    1 SFVDILLEDAASALDEKDKQLAAANKIILALSREVMRNEIQITSLTGDLTFTKKELENQRSQISEKDAKINKQEEKMFQ 79
SIR4_SID pfam16991
Sir4 SID domain; This is the Sir2 interaction domain (SID domain) of silent information ...
 746-886 3.53e-27

Sir4 SID domain; This is the Sir2 interaction domain (SID domain) of silent information regulator 4 (Sir4).


Pssm-ID: 407197  Cd Length: 159  Bit Score: 108.73  E-value: 3.53e-27
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6320433     746 KMELVYVS-DSDDSSSDNDSLTDLESLSSGESNEIKVTN--------------DLDTSAEKDQIqagkwFDPVLDWRKSD 810
Cdd:pfam16991    1 KMEIIYISdSDEESENDEASGTDEENSSSRSVDDGEISNyiknsstpltallvDSETSSEGSSI-----GDPETERKKYD 75

                           90       100       110       120       130       140       150
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 6320433     811 RELTKNILWRIADKTTYDKETITDLIEQGIPKHSYLSGNPLTSVTNDICSVENYETSSAFFYQQVHKKDRLQYLPL 886
Cdd:pfam16991   76 RQLAIRVNEKLAKKKAFSKSTISDLIESGIAPHTPLESDPYVSVTNNICSVRNYESSHAFHYQQLHKKDRLEYLPI 151
PTZ00449 PTZ00449
104 kDa microneme/rhoptry antigen; Provisional
 2-106 1.38e-03

104 kDa microneme/rhoptry antigen; Provisional


Pssm-ID: 185628 [Multi-domain]  Cd Length: 943  Bit Score: 43.14  E-value: 1.38e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6320433      2 PNDNKTPNRSSTPKFTKKPVTPND----KIPEREEKSnevKTPKIPLFTFAKSKNYSRPSTAIHTSPHQPSDVK-PTSHK 76
Cdd:PTZ00449  582 PKDPKHPKDPEEPKKPKRPRSAQRptrpKSPKLPELL---DIPKSPKRPESPKSPKRPPPPQRPSSPERPEGPKiIKSPK 658

                          90       100       110
                  ....*....|....*....|....*....|
gi 6320433     77 QLQQPKsSPLKKNNYNSFPHSNLEKISNSK 106
Cdd:PTZ00449  659 PPKSPK-PPFDPKFKEKFYDDYLDAAAKSK 687
CwlO1 COG3883
Uncharacterized N-terminal coiled-coil domain of peptidoglycan hydrolase CwlO [Function ...
 1275-1347 9.23e-03

Uncharacterized N-terminal coiled-coil domain of peptidoglycan hydrolase CwlO [Function unknown];


Pssm-ID: 443091 [Multi-domain]  Cd Length: 379  Bit Score: 40.20  E-value: 9.23e-03
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 6320433  1275 DIVLSKAASALDEKEKQLAVANEIIRSLSDEVMRNEIRITSLQGDLTFTKKCLENARSQISEKDAKINKLMEK 1347
Cdd:COG3883   15 DPQIQAKQKELSELQAELEAAQAELDALQAELEELNEEYNELQAELEALQAEIDKLQAEIAEAEAEIEERREE 87
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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