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Conserved domains on  [gi|1908918791|ref|NP_001374134|]
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DEP domain-containing protein 4 isoform 6 [Homo sapiens]

Protein Classification

Rho GTPase-activating protein( domain architecture ID 10138380)

Rho GTPase-activating protein for Rho/Rac/Cdc42-like small GTPases that act as molecular switches, active in their GTP-bound form but inactive when bound to GDP

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
RhoGAP_BRCC3-like cd04405
RhoGAP_BRCC3-like: RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain ...
 233-462 2.95e-61

RhoGAP_BRCC3-like: RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain of BRCC3-like proteins. This subgroup also contains two groups of closely related proteins, BRCC3 and DEPDC7, which both contain a C-terminal RhoGAP-like domain and an N-terminal DEP (Disheveled, Egl-10, and Pleckstrin) domain. The function(s) of BRCC3 and DEPDC7 are unknown. Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude.


:

Pssm-ID: 239870  Cd Length: 235  Bit Score: 201.02  E-value: 2.95e-61
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1908918791 233 EDVWKEQTLLCLLQLIHLPFLDNILEP-PVKTQNLQLNKEEDlVITNTCLDRELIPSLCLPEIDNWLNAAIECLEYFPDQ 311
Cdd:cd04405     7 EEIWKEQTLIRLLQLVGLPLLEELLDPaLVNPKHISYNMDPD-VYTSNYLDREVVKLFSKSQLDHWLLSAMDCLANWPDQ 85

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1908918791 312 LIVTVSQQLMQnrnEETRLNSQKKILFDVIAKYYAQERDCLLTDEYFDIHSGIIELLENEKRAEALEATQLYLRLLLLNI 391
Cdd:cd04405    86 LVVDVSRPLYS---QHDMLSGFKRLLFKTIAKYYGQLKEPLLTFHLFDIFVGILELLGNGKEEVALEALQLCLLLLPPAS 162

                         170       180       190       200       210       220       230
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 1908918791 392 REELRRLLTFMAMASEPNAYKLQKQYDNKTVVLKTLAKSVLQAKSLLKVRAEQLVWFPLEYHSELFKTPVT 462
Cdd:cd04405   163 RRELRRLLRFMARAAKNDMPRLHKEIENRMLVKQTFSRAILCSKDLDEGLADLLVLFLMDHHQDIFKVPGS 233
DEP_DEPDC4 cd04446
DEP (Dishevelled, Egl-10, and Pleckstrin) domain found in DEPDC4-like proteins. DEPDC4 is a ...
 64-159 2.04e-43

DEP (Dishevelled, Egl-10, and Pleckstrin) domain found in DEPDC4-like proteins. DEPDC4 is a DEP domain containing protein of unknown function.


:

Pssm-ID: 239893  Cd Length: 95  Bit Score: 149.05  E-value: 2.04e-43
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1908918791  64 WDGIIHSLQAQVEIKRRRHHLQTYKDCFTGSDAVDVVLSHLMQNTCLSSNDISCLKGVHLCQVLMNHKVFEPVGMkKLFK 143
Cdd:cd04446     1 WNSIIDALQTQVEVKKRRHNLKSYHDCFLGSEAVDVVLAHLMQNKYFGDVDVPRAKAVRLCQALMDCRVFEAVGT-KVFK 79

                          90
                  ....*....|....*.
gi 1908918791 144 KEKELEFEDSNISLYR 159
Cdd:cd04446    80 KKKRAVFEDSSSSLYR 95
 
Name Accession Description Interval E-value
RhoGAP_BRCC3-like cd04405
RhoGAP_BRCC3-like: RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain ...
 233-462 2.95e-61

RhoGAP_BRCC3-like: RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain of BRCC3-like proteins. This subgroup also contains two groups of closely related proteins, BRCC3 and DEPDC7, which both contain a C-terminal RhoGAP-like domain and an N-terminal DEP (Disheveled, Egl-10, and Pleckstrin) domain. The function(s) of BRCC3 and DEPDC7 are unknown. Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude.


Pssm-ID: 239870  Cd Length: 235  Bit Score: 201.02  E-value: 2.95e-61
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1908918791 233 EDVWKEQTLLCLLQLIHLPFLDNILEP-PVKTQNLQLNKEEDlVITNTCLDRELIPSLCLPEIDNWLNAAIECLEYFPDQ 311
Cdd:cd04405     7 EEIWKEQTLIRLLQLVGLPLLEELLDPaLVNPKHISYNMDPD-VYTSNYLDREVVKLFSKSQLDHWLLSAMDCLANWPDQ 85

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1908918791 312 LIVTVSQQLMQnrnEETRLNSQKKILFDVIAKYYAQERDCLLTDEYFDIHSGIIELLENEKRAEALEATQLYLRLLLLNI 391
Cdd:cd04405    86 LVVDVSRPLYS---QHDMLSGFKRLLFKTIAKYYGQLKEPLLTFHLFDIFVGILELLGNGKEEVALEALQLCLLLLPPAS 162

                         170       180       190       200       210       220       230
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 1908918791 392 REELRRLLTFMAMASEPNAYKLQKQYDNKTVVLKTLAKSVLQAKSLLKVRAEQLVWFPLEYHSELFKTPVT 462
Cdd:cd04405   163 RRELRRLLRFMARAAKNDMPRLHKEIENRMLVKQTFSRAILCSKDLDEGLADLLVLFLMDHHQDIFKVPGS 233
DEP_DEPDC4 cd04446
DEP (Dishevelled, Egl-10, and Pleckstrin) domain found in DEPDC4-like proteins. DEPDC4 is a ...
 64-159 2.04e-43

DEP (Dishevelled, Egl-10, and Pleckstrin) domain found in DEPDC4-like proteins. DEPDC4 is a DEP domain containing protein of unknown function.


Pssm-ID: 239893  Cd Length: 95  Bit Score: 149.05  E-value: 2.04e-43
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1908918791  64 WDGIIHSLQAQVEIKRRRHHLQTYKDCFTGSDAVDVVLSHLMQNTCLSSNDISCLKGVHLCQVLMNHKVFEPVGMkKLFK 143
Cdd:cd04446     1 WNSIIDALQTQVEVKKRRHNLKSYHDCFLGSEAVDVVLAHLMQNKYFGDVDVPRAKAVRLCQALMDCRVFEAVGT-KVFK 79

                          90
                  ....*....|....*.
gi 1908918791 144 KEKELEFEDSNISLYR 159
Cdd:cd04446    80 KKKRAVFEDSSSSLYR 95
DEP pfam00610
Domain found in Dishevelled, Egl-10, and Pleckstrin (DEP); The DEP domain is responsible for ...
 75-160 2.11e-14

Domain found in Dishevelled, Egl-10, and Pleckstrin (DEP); The DEP domain is responsible for mediating intracellular protein targeting and regulation of protein stability in the cell. The DEP domain is present in a number of signaling molecules, including Regulator of G protein Signaling (RGS) proteins, and has been implicated in membrane targeting. New findings in yeast, however, demonstrate a major role for a DEP domain in mediating the interaction of an RGS protein to the C-terminal tail of a GPCR, thus placing RGS in close proximity with its substrate G protein alpha subunit.


Pssm-ID: 459867  Cd Length: 71  Bit Score: 68.00  E-value: 2.11e-14
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1908918791  75 VEIKRRRHHLQTYKDCFTGSDAVDVVLSHLMqntclssnDISCLKGVHLCQVLMNHKVFEPVGmkklfkkEKELEFEDSN 154
Cdd:pfam00610   2 VKLKDRRKHLKTYPNCFTGSEAVDWLMDNLE--------IITREEAVELGQLLLDQGLIHHVG-------DKHGLFKDSY 66


                  ....*.
gi 1908918791 155 iSLYRF 160
Cdd:pfam00610  67 -YFYRF 71
DEP smart00049
Domain found in Dishevelled, Egl-10, and Pleckstrin; Domain of unknown function present in ...
 75-136 6.41e-11

Domain found in Dishevelled, Egl-10, and Pleckstrin; Domain of unknown function present in signalling proteins that contain PH, rasGEF, rhoGEF, rhoGAP, RGS, PDZ domains. DEP domain in Drosophila dishevelled is essential to rescue planar polarity defects and induce JNK signalling (Cell 94, 109-118).


Pssm-ID: 214489  Cd Length: 77  Bit Score: 58.45  E-value: 6.41e-11
                           10        20        30        40        50        60
                   ....*....|....*....|....*....|....*....|....*....|....*....|..
gi 1908918791   75 VEIKRRRHHLQTYKDCFTGSDAVDVvlshLMQNTCLSSNDisclKGVHLCQVLMNHKVFEPV 136
Cdd:smart00049   5 LKLRDRKYFLKTYPNCFTGSELVDW----LMDNLEIIDRE----EAVHLGQLLLDEGLIHHV 58
 
Name Accession Description Interval E-value
RhoGAP_BRCC3-like cd04405
RhoGAP_BRCC3-like: RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain ...
 233-462 2.95e-61

RhoGAP_BRCC3-like: RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain of BRCC3-like proteins. This subgroup also contains two groups of closely related proteins, BRCC3 and DEPDC7, which both contain a C-terminal RhoGAP-like domain and an N-terminal DEP (Disheveled, Egl-10, and Pleckstrin) domain. The function(s) of BRCC3 and DEPDC7 are unknown. Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude.


Pssm-ID: 239870  Cd Length: 235  Bit Score: 201.02  E-value: 2.95e-61
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1908918791 233 EDVWKEQTLLCLLQLIHLPFLDNILEP-PVKTQNLQLNKEEDlVITNTCLDRELIPSLCLPEIDNWLNAAIECLEYFPDQ 311
Cdd:cd04405     7 EEIWKEQTLIRLLQLVGLPLLEELLDPaLVNPKHISYNMDPD-VYTSNYLDREVVKLFSKSQLDHWLLSAMDCLANWPDQ 85

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1908918791 312 LIVTVSQQLMQnrnEETRLNSQKKILFDVIAKYYAQERDCLLTDEYFDIHSGIIELLENEKRAEALEATQLYLRLLLLNI 391
Cdd:cd04405    86 LVVDVSRPLYS---QHDMLSGFKRLLFKTIAKYYGQLKEPLLTFHLFDIFVGILELLGNGKEEVALEALQLCLLLLPPAS 162

                         170       180       190       200       210       220       230
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 1908918791 392 REELRRLLTFMAMASEPNAYKLQKQYDNKTVVLKTLAKSVLQAKSLLKVRAEQLVWFPLEYHSELFKTPVT 462
Cdd:cd04405   163 RRELRRLLRFMARAAKNDMPRLHKEIENRMLVKQTFSRAILCSKDLDEGLADLLVLFLMDHHQDIFKVPGS 233
DEP_DEPDC4 cd04446
DEP (Dishevelled, Egl-10, and Pleckstrin) domain found in DEPDC4-like proteins. DEPDC4 is a ...
 64-159 2.04e-43

DEP (Dishevelled, Egl-10, and Pleckstrin) domain found in DEPDC4-like proteins. DEPDC4 is a DEP domain containing protein of unknown function.


Pssm-ID: 239893  Cd Length: 95  Bit Score: 149.05  E-value: 2.04e-43
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1908918791  64 WDGIIHSLQAQVEIKRRRHHLQTYKDCFTGSDAVDVVLSHLMQNTCLSSNDISCLKGVHLCQVLMNHKVFEPVGMkKLFK 143
Cdd:cd04446     1 WNSIIDALQTQVEVKKRRHNLKSYHDCFLGSEAVDVVLAHLMQNKYFGDVDVPRAKAVRLCQALMDCRVFEAVGT-KVFK 79

                          90
                  ....*....|....*.
gi 1908918791 144 KEKELEFEDSNISLYR 159
Cdd:cd04446    80 KKKRAVFEDSSSSLYR 95
DEP pfam00610
Domain found in Dishevelled, Egl-10, and Pleckstrin (DEP); The DEP domain is responsible for ...
 75-160 2.11e-14

Domain found in Dishevelled, Egl-10, and Pleckstrin (DEP); The DEP domain is responsible for mediating intracellular protein targeting and regulation of protein stability in the cell. The DEP domain is present in a number of signaling molecules, including Regulator of G protein Signaling (RGS) proteins, and has been implicated in membrane targeting. New findings in yeast, however, demonstrate a major role for a DEP domain in mediating the interaction of an RGS protein to the C-terminal tail of a GPCR, thus placing RGS in close proximity with its substrate G protein alpha subunit.


Pssm-ID: 459867  Cd Length: 71  Bit Score: 68.00  E-value: 2.11e-14
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1908918791  75 VEIKRRRHHLQTYKDCFTGSDAVDVVLSHLMqntclssnDISCLKGVHLCQVLMNHKVFEPVGmkklfkkEKELEFEDSN 154
Cdd:pfam00610   2 VKLKDRRKHLKTYPNCFTGSEAVDWLMDNLE--------IITREEAVELGQLLLDQGLIHHVG-------DKHGLFKDSY 66


                  ....*.
gi 1908918791 155 iSLYRF 160
Cdd:pfam00610  67 -YFYRF 71
DEP cd04371
DEP domain, named after Dishevelled, Egl-10, and Pleckstrin, where this domain was first ...
 62-137 2.58e-14

DEP domain, named after Dishevelled, Egl-10, and Pleckstrin, where this domain was first discovered. The function of this domain is still not clear, but it is believed to be important for the membrane association of the signaling proteins in which it is present. New studies show that the DEP domain of Sst2, a yeast RGS protein is necessary and sufficient for receptor interaction.


Pssm-ID: 239836  Cd Length: 81  Bit Score: 68.14  E-value: 2.58e-14
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 1908918791  62 QLWDGIIHSLQAqVEIKRRRHHLQTYKDCFTGSDAVDVVLSHLmqntclssNDISCLKGVHLCQVLMNHKVFEPVG 137
Cdd:cd04371     1 DLVRIMLDSDSG-VPIKDRKYHLKTYPNCFTGSELVDWLLDNL--------EAITREEAVELGQALLKHGLIHHVS 67
DEP smart00049
Domain found in Dishevelled, Egl-10, and Pleckstrin; Domain of unknown function present in ...
 75-136 6.41e-11

Domain found in Dishevelled, Egl-10, and Pleckstrin; Domain of unknown function present in signalling proteins that contain PH, rasGEF, rhoGEF, rhoGAP, RGS, PDZ domains. DEP domain in Drosophila dishevelled is essential to rescue planar polarity defects and induce JNK signalling (Cell 94, 109-118).


Pssm-ID: 214489  Cd Length: 77  Bit Score: 58.45  E-value: 6.41e-11
                           10        20        30        40        50        60
                   ....*....|....*....|....*....|....*....|....*....|....*....|..
gi 1908918791   75 VEIKRRRHHLQTYKDCFTGSDAVDVvlshLMQNTCLSSNDisclKGVHLCQVLMNHKVFEPV 136
Cdd:smart00049   5 LKLRDRKYFLKTYPNCFTGSELVDW----LMDNLEIIDRE----EAVHLGQLLLDEGLIHHV 58
DEP_BRCC3 cd04447
DEP (Dishevelled, Egl-10, and Pleckstrin) domain found in BBRC3-like proteins. BBRC3, also ...
 62-160 3.73e-04

DEP (Dishevelled, Egl-10, and Pleckstrin) domain found in BBRC3-like proteins. BBRC3, also known as DEPDC1B, is a DEP containing protein of unknown function.


Pssm-ID: 239894  Cd Length: 92  Bit Score: 39.44  E-value: 3.73e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1908918791  62 QLWDGIIHSLQAQVEIKRRRHHLQTYKDCFTGSDAVDvVLSHLMQNTCLSSNDISCLKGVHLCQVLMNHKVFEPVgmkkl 141
Cdd:cd04447     1 KLWNEVTELFRAGMPLRKHRQHFKSYENCFTASEAVD-WLHELLRSNSNFGPEVTRQQTVQLLKKFLKNHVIEDI----- 74

                          90
                  ....*....|....*....
gi 1908918791 142 fKKEKELEFEDSNISLYRF 160
Cdd:cd04447    75 -KGRWGKEDLEDNNHLYRF 92
DEP_1_DEP6 cd04442
DEP (Dishevelled, Egl-10, and Pleckstrin) domain 1 found in DEP6-like proteins. DEP6 proteins ...
 77-103 9.09e-04

DEP (Dishevelled, Egl-10, and Pleckstrin) domain 1 found in DEP6-like proteins. DEP6 proteins contain two DEP and a PDZ domain. Their function is unknown.


Pssm-ID: 239889 [Multi-domain]  Cd Length: 82  Bit Score: 38.34  E-value: 9.09e-04
                          10        20
                  ....*....|....*....|....*..
gi 1908918791  77 IKRRRHHLQTYKDCFTGSDAVDVVLSH 103
Cdd:cd04442    15 IKDRRHHLRTYPNCFVGKELIDWLIEH 41
DEP_2_P-Rex cd04440
DEP (Dishevelled, Egl-10, and Pleckstrin) domain 2 found in P-Rex-like proteins. The P-Rex ...
 68-134 2.73e-03

DEP (Dishevelled, Egl-10, and Pleckstrin) domain 2 found in P-Rex-like proteins. The P-Rex family is the guanine-nucleotide exchange factor (GEF) for the small GTPase Rac that contains an N-terminal RhoGEF domain, two DEP and PDZ domains. Rac-GEF activity is stimulated by phosphatidylinositol (3,4,5)-trisphosphate (PtdIns(3,4,5)P3), a lipid second messenger, and the G beta-gamma subunits of heterotrimeric G proteins. The DEP domains are not involved in mediating these stimuli, but may be of importance for basal and stimulated levels Rac-GEF activity.


Pssm-ID: 239887  Cd Length: 93  Bit Score: 37.21  E-value: 2.73e-03
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 1908918791  68 IHSLQAQVeIKRRRHHLQTYKDCFTGSDAVDVVLShlmQNTCLSSNDISCLkGVHLCQVLMNHKVFE 134
Cdd:cd04440    16 LHSLFTPV-VKDRDYHLKTYKSVVPASKLVDWLLA---QGDCRTREEAVIL-GVGLCNNGFMHHVLE 77
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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