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Conserved domains on  [gi|1831506055|ref|NP_001367991|]
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VASt domain-containing protein [Caenorhabditis elegans]

Protein Classification

GRAM and VASt domain-containing protein( domain architecture ID 10881580)

GRAM and VASt domain-containing protein such as mammalian Aster proteins (or GRAM domain-containing proteins), which act as cholesterol transporters that mediate non-vesicular transport of cholesterol from the plasma membrane (PM) to the endoplasmic reticulum (ER)

CATH:  2.30.29.30
Gene Ontology:  GO:0016020|GO:0008289|GO:0006869
PubMed:  18201690|24965341|26001273|11050430
SCOP:  4000903

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
PH-GRAM_GRAMDC cd13220
GRAM domain-containing protein (GRAMDC) Pleckstrin Homology-Glucosyltransferases, Rab-like ...
 88-180 6.40e-45

GRAM domain-containing protein (GRAMDC) Pleckstrin Homology-Glucosyltransferases, Rab-like GTPase activators and Myotubularins (PH-GRAM) domain; The GRAMDC proteins are membrane proteins. Nothing is known about its function. Members include: GRAMDC1A, GRAMDC1B, GRAMDC1C, GRAMDC2, GRAMDC3, GRAMDC4, and GRAMDC-like proteins. All of the members, except for GRAMDC4 are included in this hierarchy. Each contains a single PH-GRAM domain at their N-terminus. The GRAM domain is found in glucosyltransferases, myotubularins and other putative membrane-associated proteins. The GRAM domain is part of a larger motif with a pleckstrin homology (PH) domain fold.


:

Pssm-ID: 275406  Cd Length: 94  Bit Score: 154.59  E-value: 6.40e-45
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1831506055  88 ENDQFLASYSCAYQREILAQGRMFISQFNVCFYANIIGWETTLVIPMKEISLVKKMKAAFIFPNSIQIERDTkEKYFFAS 167
Cdd:cd13220     1 EDERLIDDFSCALQRDILLQGRLYISENHLCFYSNIFGWETKLVIPFKDITSIEKKKTALIFPNAIEITTKG-EKYFFTS 79

                          90
                  ....*....|...
gi 1831506055 168 FINRDKSYQVLTT 180
Cdd:cd13220    80 FLSRDSAYKLLTR 92
VASt pfam16016
VAD1 Analog of StAR-related lipid transfer domain; The VASt (VAD1 Analog of StAR-related lipid ...
 305-453 1.36e-40

VAD1 Analog of StAR-related lipid transfer domain; The VASt (VAD1 Analog of StAR-related lipid transfer) domain is conserved across eukaryotes and is structurally related to Bet v1-like domains, including START lipid-binding domains. The 190-amino acid VASt domain is predominantly associated with lipid binding domains such as GRAM pfam02893, C2 and PH domains. The VASt domain is likely to have a function in binding large hydrophobic ligands and may be specific for sterol. The predicted structure of the VASt domain is a two-layer sandwich alpha beta fold, also called 'helix grip fold', containing three alpha helices (alpha1 to 3), six beta-sheets (beta1 to 6) and two loops (omega1 and 2) numbered from N to C terminus. Some proteins known to contain a VASt domain are : Plant vascular associated death1 (VAD1), a regulator of programmed cell death (PCD) harboring a GRAM putative lipid-binding domain. Yeast SNF1 Interacting Protein 3 (SIP3), may be involved in sterol transfer between intracellular membranes. Yeast Suicide Protein 1 (YSP1), a mitochondrial protein specifically required for the mitochondrial thread-grain transition, de-energization, and the cell death. May be involved in sterol transfer between intracellular membranes. Yeast Suicide Protein 2 (YSP2), a mitochondrial membrane protein involved in mitochondrial fragmentation and may be involved in sterol transfer between intracellular membranes.It is also found in human GramD1a-c.


:

Pssm-ID: 464975  Cd Length: 147  Bit Score: 144.67  E-value: 1.36e-40
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1831506055 305 LIMDKEFKVSVEKFYELLFTDN-EFLTMLNQKTKTAEYVAATWVRDHQGDNTRTCTYTVSLAHAMAPKAIIVNEKQILtH 383
Cdd:pfam16016   1 VLLDEVFPISVGKLFELLFGDDsSFLQKFLEERGDTDISVGPWEPDEEGGLTREISYTKPLNGSIGPKQTKCTETQTI-L 79

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1831506055 384 YPNPKQGIMMQKETQNSGVPYSDNFTVNCRYCISRTGPTSCRIKVHGGVMYKKSTWavVKTFIEKGTHQG 453
Cdd:pfam16016  80 LEHDDLEVYVVTTTKTPDVPYGDSFSVETRYCITWGSNNSTRLQVSTGVEWTKSSW--LKGKIEKGAIDG 147
 
Name Accession Description Interval E-value
PH-GRAM_GRAMDC cd13220
GRAM domain-containing protein (GRAMDC) Pleckstrin Homology-Glucosyltransferases, Rab-like ...
 88-180 6.40e-45

GRAM domain-containing protein (GRAMDC) Pleckstrin Homology-Glucosyltransferases, Rab-like GTPase activators and Myotubularins (PH-GRAM) domain; The GRAMDC proteins are membrane proteins. Nothing is known about its function. Members include: GRAMDC1A, GRAMDC1B, GRAMDC1C, GRAMDC2, GRAMDC3, GRAMDC4, and GRAMDC-like proteins. All of the members, except for GRAMDC4 are included in this hierarchy. Each contains a single PH-GRAM domain at their N-terminus. The GRAM domain is found in glucosyltransferases, myotubularins and other putative membrane-associated proteins. The GRAM domain is part of a larger motif with a pleckstrin homology (PH) domain fold.


Pssm-ID: 275406  Cd Length: 94  Bit Score: 154.59  E-value: 6.40e-45
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1831506055  88 ENDQFLASYSCAYQREILAQGRMFISQFNVCFYANIIGWETTLVIPMKEISLVKKMKAAFIFPNSIQIERDTkEKYFFAS 167
Cdd:cd13220     1 EDERLIDDFSCALQRDILLQGRLYISENHLCFYSNIFGWETKLVIPFKDITSIEKKKTALIFPNAIEITTKG-EKYFFTS 79

                          90
                  ....*....|...
gi 1831506055 168 FINRDKSYQVLTT 180
Cdd:cd13220    80 FLSRDSAYKLLTR 92
VASt pfam16016
VAD1 Analog of StAR-related lipid transfer domain; The VASt (VAD1 Analog of StAR-related lipid ...
 305-453 1.36e-40

VAD1 Analog of StAR-related lipid transfer domain; The VASt (VAD1 Analog of StAR-related lipid transfer) domain is conserved across eukaryotes and is structurally related to Bet v1-like domains, including START lipid-binding domains. The 190-amino acid VASt domain is predominantly associated with lipid binding domains such as GRAM pfam02893, C2 and PH domains. The VASt domain is likely to have a function in binding large hydrophobic ligands and may be specific for sterol. The predicted structure of the VASt domain is a two-layer sandwich alpha beta fold, also called 'helix grip fold', containing three alpha helices (alpha1 to 3), six beta-sheets (beta1 to 6) and two loops (omega1 and 2) numbered from N to C terminus. Some proteins known to contain a VASt domain are : Plant vascular associated death1 (VAD1), a regulator of programmed cell death (PCD) harboring a GRAM putative lipid-binding domain. Yeast SNF1 Interacting Protein 3 (SIP3), may be involved in sterol transfer between intracellular membranes. Yeast Suicide Protein 1 (YSP1), a mitochondrial protein specifically required for the mitochondrial thread-grain transition, de-energization, and the cell death. May be involved in sterol transfer between intracellular membranes. Yeast Suicide Protein 2 (YSP2), a mitochondrial membrane protein involved in mitochondrial fragmentation and may be involved in sterol transfer between intracellular membranes.It is also found in human GramD1a-c.


Pssm-ID: 464975  Cd Length: 147  Bit Score: 144.67  E-value: 1.36e-40
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1831506055 305 LIMDKEFKVSVEKFYELLFTDN-EFLTMLNQKTKTAEYVAATWVRDHQGDNTRTCTYTVSLAHAMAPKAIIVNEKQILtH 383
Cdd:pfam16016   1 VLLDEVFPISVGKLFELLFGDDsSFLQKFLEERGDTDISVGPWEPDEEGGLTREISYTKPLNGSIGPKQTKCTETQTI-L 79

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1831506055 384 YPNPKQGIMMQKETQNSGVPYSDNFTVNCRYCISRTGPTSCRIKVHGGVMYKKSTWavVKTFIEKGTHQG 453
Cdd:pfam16016  80 LEHDDLEVYVVTTTKTPDVPYGDSFSVETRYCITWGSNNSTRLQVSTGVEWTKSSW--LKGKIEKGAIDG 147
GRAM pfam02893
GRAM domain; The GRAM domain is found in in glucosyltransferases, myotubularins and other ...
 76-185 2.73e-21

GRAM domain; The GRAM domain is found in in glucosyltransferases, myotubularins and other putative membrane-associated proteins. Note the alignment is lacking the last two beta strands and alpha helix.


Pssm-ID: 397160  Cd Length: 112  Bit Score: 89.35  E-value: 2.73e-21
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1831506055  76 FKKIFVdkglIEENDQFLASYSCAYQRE-ILAQGRMFISQFNVCFYANIIGWETTLVIPMKEISLVKKMK-AAFIFPNSI 153
Cdd:pfam02893   3 FRKKFK----LPPEERLIASYSCYLNRDgGPVQGRLYLTNYRLCFRSLPKGWSTKVVIPLVDIEEIEKLKgGANLFPNGI 78

                          90       100       110
                  ....*....|....*....|....*....|..
gi 1831506055 154 QIERDTKEKYFFASFINRDKSYQVLTTAHQKV 185
Cdd:pfam02893  79 QVETGSNDKFSFAGFVTRDEAIEFILALLKNA 110
GRAM smart00568
domain in glucosyltransferases, myotubularins and other putative membrane-associated proteins;
88-144 2.47e-18

domain in glucosyltransferases, myotubularins and other putative membrane-associated proteins;


Pssm-ID: 214725 [Multi-domain]  Cd Length: 60  Bit Score: 79.17  E-value: 2.47e-18
                           10        20        30        40        50
                   ....*....|....*....|....*....|....*....|....*....|....*..
gi 1831506055   88 ENDQFLASYSCAYQREILAQGRMFISQFNVCFYANIIGWETTLVIPMKEISLVKKMK 144
Cdd:smart00568   4 EEEKLIADYSCYLSRTGPVQGRLYISNYRLCFRSNLPGKLTKVVIPLADITRIEKST 60
 
Name Accession Description Interval E-value
PH-GRAM_GRAMDC cd13220
GRAM domain-containing protein (GRAMDC) Pleckstrin Homology-Glucosyltransferases, Rab-like ...
 88-180 6.40e-45

GRAM domain-containing protein (GRAMDC) Pleckstrin Homology-Glucosyltransferases, Rab-like GTPase activators and Myotubularins (PH-GRAM) domain; The GRAMDC proteins are membrane proteins. Nothing is known about its function. Members include: GRAMDC1A, GRAMDC1B, GRAMDC1C, GRAMDC2, GRAMDC3, GRAMDC4, and GRAMDC-like proteins. All of the members, except for GRAMDC4 are included in this hierarchy. Each contains a single PH-GRAM domain at their N-terminus. The GRAM domain is found in glucosyltransferases, myotubularins and other putative membrane-associated proteins. The GRAM domain is part of a larger motif with a pleckstrin homology (PH) domain fold.


Pssm-ID: 275406  Cd Length: 94  Bit Score: 154.59  E-value: 6.40e-45
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1831506055  88 ENDQFLASYSCAYQREILAQGRMFISQFNVCFYANIIGWETTLVIPMKEISLVKKMKAAFIFPNSIQIERDTkEKYFFAS 167
Cdd:cd13220     1 EDERLIDDFSCALQRDILLQGRLYISENHLCFYSNIFGWETKLVIPFKDITSIEKKKTALIFPNAIEITTKG-EKYFFTS 79

                          90
                  ....*....|...
gi 1831506055 168 FINRDKSYQVLTT 180
Cdd:cd13220    80 FLSRDSAYKLLTR 92
VASt pfam16016
VAD1 Analog of StAR-related lipid transfer domain; The VASt (VAD1 Analog of StAR-related lipid ...
 305-453 1.36e-40

VAD1 Analog of StAR-related lipid transfer domain; The VASt (VAD1 Analog of StAR-related lipid transfer) domain is conserved across eukaryotes and is structurally related to Bet v1-like domains, including START lipid-binding domains. The 190-amino acid VASt domain is predominantly associated with lipid binding domains such as GRAM pfam02893, C2 and PH domains. The VASt domain is likely to have a function in binding large hydrophobic ligands and may be specific for sterol. The predicted structure of the VASt domain is a two-layer sandwich alpha beta fold, also called 'helix grip fold', containing three alpha helices (alpha1 to 3), six beta-sheets (beta1 to 6) and two loops (omega1 and 2) numbered from N to C terminus. Some proteins known to contain a VASt domain are : Plant vascular associated death1 (VAD1), a regulator of programmed cell death (PCD) harboring a GRAM putative lipid-binding domain. Yeast SNF1 Interacting Protein 3 (SIP3), may be involved in sterol transfer between intracellular membranes. Yeast Suicide Protein 1 (YSP1), a mitochondrial protein specifically required for the mitochondrial thread-grain transition, de-energization, and the cell death. May be involved in sterol transfer between intracellular membranes. Yeast Suicide Protein 2 (YSP2), a mitochondrial membrane protein involved in mitochondrial fragmentation and may be involved in sterol transfer between intracellular membranes.It is also found in human GramD1a-c.


Pssm-ID: 464975  Cd Length: 147  Bit Score: 144.67  E-value: 1.36e-40
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1831506055 305 LIMDKEFKVSVEKFYELLFTDN-EFLTMLNQKTKTAEYVAATWVRDHQGDNTRTCTYTVSLAHAMAPKAIIVNEKQILtH 383
Cdd:pfam16016   1 VLLDEVFPISVGKLFELLFGDDsSFLQKFLEERGDTDISVGPWEPDEEGGLTREISYTKPLNGSIGPKQTKCTETQTI-L 79

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1831506055 384 YPNPKQGIMMQKETQNSGVPYSDNFTVNCRYCISRTGPTSCRIKVHGGVMYKKSTWavVKTFIEKGTHQG 453
Cdd:pfam16016  80 LEHDDLEVYVVTTTKTPDVPYGDSFSVETRYCITWGSNNSTRLQVSTGVEWTKSSW--LKGKIEKGAIDG 147
GRAM pfam02893
GRAM domain; The GRAM domain is found in in glucosyltransferases, myotubularins and other ...
 76-185 2.73e-21

GRAM domain; The GRAM domain is found in in glucosyltransferases, myotubularins and other putative membrane-associated proteins. Note the alignment is lacking the last two beta strands and alpha helix.


Pssm-ID: 397160  Cd Length: 112  Bit Score: 89.35  E-value: 2.73e-21
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1831506055  76 FKKIFVdkglIEENDQFLASYSCAYQRE-ILAQGRMFISQFNVCFYANIIGWETTLVIPMKEISLVKKMK-AAFIFPNSI 153
Cdd:pfam02893   3 FRKKFK----LPPEERLIASYSCYLNRDgGPVQGRLYLTNYRLCFRSLPKGWSTKVVIPLVDIEEIEKLKgGANLFPNGI 78

                          90       100       110
                  ....*....|....*....|....*....|..
gi 1831506055 154 QIERDTKEKYFFASFINRDKSYQVLTTAHQKV 185
Cdd:pfam02893  79 QVETGSNDKFSFAGFVTRDEAIEFILALLKNA 110
GRAM smart00568
domain in glucosyltransferases, myotubularins and other putative membrane-associated proteins;
88-144 2.47e-18

domain in glucosyltransferases, myotubularins and other putative membrane-associated proteins;


Pssm-ID: 214725 [Multi-domain]  Cd Length: 60  Bit Score: 79.17  E-value: 2.47e-18
                           10        20        30        40        50
                   ....*....|....*....|....*....|....*....|....*....|....*..
gi 1831506055   88 ENDQFLASYSCAYQREILAQGRMFISQFNVCFYANIIGWETTLVIPMKEISLVKKMK 144
Cdd:smart00568   4 EEEKLIADYSCYLSRTGPVQGRLYISNYRLCFRSNLPGKLTKVVIPLADITRIEKST 60
PH-GRAM_C2-GRAM cd13219
C2 and GRAM domain-containing protein Pleckstrin Homology-Glucosyltransferases, Rab-like ...
 90-181 3.78e-15

C2 and GRAM domain-containing protein Pleckstrin Homology-Glucosyltransferases, Rab-like GTPase activators and Myotubularins (PH-GRAM) domain; C2GRAM contains two N-terminal C2 domains followed by a single PH-GRAM domain. Since it contains both of these domains it is assumed that this gene cross-links both calcium and phosphoinositide signaling pathways. In general he C2 domain is involved in binding phospholipids in a calcium dependent manner or calcium independent manner. The GRAM domain is found in glucosyltransferases, myotubularins and other putative membrane-associated proteins. The GRAM domain is part of a larger motif with a pleckstrin homology (PH) domain fold.


Pssm-ID: 270039 [Multi-domain]  Cd Length: 111  Bit Score: 71.73  E-value: 3.78e-15
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1831506055  90 DQFLA-SYSCAYQREILAQGRMFISQFNVCFYANIIGWETTLVIPMKEISLVKKMKAAFIFPNSIQIER----------- 157
Cdd:cd13219     1 EEFLInDFSCALKRKFLYQGRMFLSARHIGFHSNVFGKKTKFVFLWEDIEEIQESPHSLINPAIVITLRkgrgldagqgv 80

                          90       100
                  ....*....|....*....|....*....
gi 1831506055 158 -----DTKEKYFFASFINRDKSYQVLTTA 181
Cdd:cd13219    81 ppidpDGRLKYKFASFVNRNHAFRTLMRL 109
PH-GRAM2_AGT26 cd13216
Autophagy-related protein 26/Sterol 3-beta-glucosyltransferase Pleckstrin homology (PH) domain, ...
 89-178 6.87e-10

Autophagy-related protein 26/Sterol 3-beta-glucosyltransferase Pleckstrin homology (PH) domain, repeat 2; ATG26 (also called UGT51/UDP-glycosyltransferase 51), a member of the glycosyltransferase 28 family, resulting in the biosynthesis of sterol glucoside. ATG26 in decane metabolism and autophagy. There are 32 known autophagy-related (ATG) proteins, 17 are components of the core autophagic machinery essential for all autophagy-related pathways and 15 are the additional components required only for certain pathways or species. The core autophagic machinery includes 1) the ATG9 cycling system (ATG1, ATG2, ATG9, ATG13, ATG18, and ATG27), 2) the phosphatidylinositol 3-kinase complex (ATG6/VPS30, ATG14, VPS15, and ATG34), and 3) the ubiquitin-like protein system (ATG3, ATG4, ATG5, ATG7, ATG8, ATG10, ATG12, and ATG16). Less is known about how the core machinery is adapted or modulated with additional components to accommodate the nonselective sequestration of bulk cytosol (autophagosome formation) or selective sequestration of specific cargos (Cvt vesicle, pexophagosome, or bacteria-containing autophagosome formation). The pexophagosome-specific additions include the ATG30-ATG11-ATG17 receptor-adaptors complex, the coiled-coil protein ATG25, and the sterol glucosyltransferase ATG26. ATG26 is necessary for the degradation of medium peroxisomes. It contains 2 GRAM domains and a single PH domain. PH domains are only found in eukaryotes. They share little sequence conservation, but all have a common fold, which is electrostatically polarized. PH domains also have diverse functions. They are often involved in targeting proteins to the plasma membrane, but few display strong specificity in lipid binding. Any specificity is usually determined by loop regions or insertions in the N-terminus of the domain, which are not conserved across all PH domains. PH domains are found in cellular signaling proteins such as serine/threonine kinase, tyrosine kinases, regulators of G-proteins, endocytotic GTPases, adaptors, as well as cytoskeletal associated molecules and in lipid associated enzymes.


Pssm-ID: 275403  Cd Length: 93  Bit Score: 56.05  E-value: 6.87e-10
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1831506055  89 NDQFLASYSCAYQREILAQGRMFISQFNVCFYANIIGWETTLVIPMKEISLVKKMKaAFIFPNS-IQIERDTKEKYF--F 165
Cdd:cd13216     1 TEKLIASYYCYLIRVLPVYGKLYVSNNYLCFRSLLPGVSTKMILPLRDIENVEKEK-GFRFGYSgLVIVIKGHEELFfeF 79

                          90
                  ....*....|...
gi 1831506055 166 ASFINRDKSYQVL 178
Cdd:cd13216    80 SSKSSRDDCERLL 92
PH-GRAM1_TCB1D9_TCB1D9B cd13351
TBC1 domain family members 9 and 9B (TBC1D9 and TBC1D9B) Pleckstrin ...
 96-179 2.64e-09

TBC1 domain family members 9 and 9B (TBC1D9 and TBC1D9B) Pleckstrin Homology-Glucosyltransferases, Rab-like GTPase activators and Myotubularins (PH-GRAM) domain, repeat 1; TBC1D9 and TCB1D9B may act as a GTPase-activating proteins for Rab family protein(s). TBC1D9 and TCB1D9B contain two N-terminal PH-GRAM domain and a C-terminal Rab-GTPase-TBC (Tre-2, BUB2p, and Cdc16p) domain. This cd contains the first repeat of the PH-GRAM domain. The GRAM domain is found in glucosyltransferases, myotubularins and other putative membrane-associated proteins. The GRAM domain is part of a larger motif with a pleckstrin homology (PH) domain fold.


Pssm-ID: 275420  Cd Length: 99  Bit Score: 54.71  E-value: 2.64e-09
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1831506055  96 YSCAY-QREILAQGRMFISQFNVCFYANIIGWETTLVIPMKEISLVKKmKAAFIFPNSIQIerDTKEK-YFFASFINRDK 173
Cdd:cd13351     8 YSCSYwKGRVPRQGWLYLSVNHLCFYSFLLGKEAKLVIRWTDVTQLEK-NNSLLLPDSIKV--VTRDKeHYFSMFLNISE 84


                  ....*.
gi 1831506055 174 SYQVLT 179
Cdd:cd13351    85 TFKLME 90
PH-GRAM cd10570
Pleckstrin Homology-Glucosyltransferases, Rab-like GTPase activators and Myotubularins ...
 96-178 3.22e-07

Pleckstrin Homology-Glucosyltransferases, Rab-like GTPase activators and Myotubularins (PH-GRAM) domain; Myotubularin-related proteins are a subfamily of protein tyrosine phosphatases (PTPs) that dephosphorylate D3-phosphorylated inositol lipids. Mutations in this family cause the human neuromuscular disorders myotubular myopathy and type 4B Charcot-Marie-Tooth syndrome. 6 of the 13 MTMRs (MTMRs 5, 9-13) contain naturally occurring substitutions of residues required for catalysis by PTP family enzymes. Although these proteins are predicted to be enzymatically inactive, they are thought to function as antagonists of endogenous phosphatase activity or interaction modules. Most MTMRs contain a N-terminal PH-GRAM domain, a Rac-induced recruitment domain (RID) domain, a PTP domain (which may be active or inactive), a SET-interaction domain, and a C-terminal coiled-coil region. In addition some members contain DENN domain N-terminal to the PH-GRAM domain and FYVE, PDZ, and PH domains C-terminal to the coiled-coil region. The GRAM domain, found in myotubularins, glucosyltransferases, and other putative membrane-associated proteins, is part of a larger motif with a pleckstrin homology (PH) domain fold.


Pssm-ID: 275393  Cd Length: 94  Bit Score: 48.53  E-value: 3.22e-07
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1831506055  96 YSCAYQREILA-QGRMFISQFNVCFYANIIGWETTLVIPMKEISLVKKMKAAFIFPNSIQIERDTKEKYFFaSFINRDKS 174
Cdd:cd10570     8 FCCALRPRKLPlEGTLYLSTYRLIFSSKADGDETKLVIPLVDITDVEKIAGASFLPSGLIITCKDFRTIKF-SFDSEDEA 86


                  ....
gi 1831506055 175 YQVL 178
Cdd:cd10570    87 VKVI 90
PH-GRAM1_TCB1D8_TCB1D9_family cd13217
TCB1D8 and TCB1D9 family Pleckstrin Homology-Glucosyltransferases, Rab-like GTPase activators ...
 95-170 3.98e-07

TCB1D8 and TCB1D9 family Pleckstrin Homology-Glucosyltransferases, Rab-like GTPase activators and Myotubularins (PH-GRAM) domain, repeat 1; TBC1D8, TBC1D8B, TBC1D9 and TBC1D9B may act as a GTPase-activating proteins for Rab family protein(s). They all contain an N-terminal PH-GRAM domain and a C-terminal Rab-GTPase-TBC (Tre-2, BUB2p, and Cdc16p) domain. This cd contains the first repeat of the PH-GRAM domain. The GRAM domain is found in glucosyltransferases, myotubularins and other putative membrane-associated proteins. The GRAM domain is part of a larger motif with a pleckstrin homology (PH) domain fold.


Pssm-ID: 275404  Cd Length: 99  Bit Score: 48.59  E-value: 3.98e-07
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 1831506055  95 SYSCAYQR-EILAQGRMFISQFNVCFYANIIGWETTLVIPMKEISlvKKMKAAFIFPNSIQIERDTKEKYFFASFIN 170
Cdd:cd13217     7 YYSCSYWKgRVPRQGWLYLSINHLCFYSFLLGSESKLIIRWTEVS--DLERTSNTILTDTIKVNTRSKEHPFSMFLN 81
PH-GRAM1_TBC1D8B cd13350
TBC1 domain family member 8B (TBC1D8B) Pleckstrin Homology-Glucosyltransferases, Rab-like ...
 90-178 1.00e-06

TBC1 domain family member 8B (TBC1D8B) Pleckstrin Homology-Glucosyltransferases, Rab-like GTPase activators and Myotubularins (PH-GRAM) domain, repeat 1; TBC1D8B may act as a GTPase-activating protein for Rab family protein(s). TBC1D8B contains an N-terminal PH-GRAM domain and a C-terminal Rab-GTPase-TBC (Tre-2, BUB2p, and Cdc16p) domain. This cd contains the first repeat of the PH-GRAM domain. The GRAM domain is found in glucosyltransferases, myotubularins and other putative membrane-associated proteins. The GRAM domain is part of a larger motif with a pleckstrin homology (PH) domain fold.


Pssm-ID: 275419  Cd Length: 99  Bit Score: 47.23  E-value: 1.00e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1831506055  90 DQFLASYSCAYQR-EILAQGRMFISQFNVCFYANIIGWETTLVIPMKEISLVKKMKAAfIFPNSIQIeRDTKEKYFFASF 168
Cdd:cd13350     2 EKLVTYYSCSYWRgRVPCQGWLYLSTNFLSFYSFLLGSEIKLIISWDEISKLEKTSNV-ILTESIHV-CSRGEDHYFSMF 79

                          90
                  ....*....|
gi 1831506055 169 INRDKSYQVL 178
Cdd:cd13350    80 LHINETFLLM 89
PH-GRAM2_TCB1D9_TCB1D9B cd13354
TBC1 domain family members 9 and 9B (TBC1D9 and TBC1D9B) Pleckstrin ...
 108-172 1.92e-04

TBC1 domain family members 9 and 9B (TBC1D9 and TBC1D9B) Pleckstrin Homology-Glucosyltransferases, Rab-like GTPase activators and Myotubularins (PH-GRAM) domain, repeat 2; TBC1D9 and TCB1D9B may act as a GTPase-activating proteins for Rab family protein(s). TBC1D9 and TCB1D9B contain two N-terminal PH-GRAM domain and a C-terminal Rab-GTPase-TBC (Tre-2, BUB2p, and Cdc16p) domain. This cd contains the second repeat of the PH-GRAM domain. The GRAM domain is found in glucosyltransferases, myotubularins and other putative membrane-associated proteins. The GRAM domain is part of a larger motif with a pleckstrin homology (PH) domain fold.


Pssm-ID: 270161  Cd Length: 97  Bit Score: 40.71  E-value: 1.92e-04
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 1831506055 108 GRMFISQFNVCFYANIIGwETTLVIPMKEISLVKKMK-AAFIFPNSIQIERDTKEKYFFASFINRD 172
Cdd:cd13354    23 GTLYLSQNYICFTSKVRD-LVSLVIPLREVTSVEKADsSSVSLPNGILITTKSKMTFLFAQIKDRD 87
PH-GRAM1_TBC1D8 cd13349
TBC1 domain family member 8 (TBC1D8; also called Vascular Rab-GAP/TBC-containing protein) ...
 90-178 2.24e-04

TBC1 domain family member 8 (TBC1D8; also called Vascular Rab-GAP/TBC-containing protein) Pleckstrin Homology-Glucosyltransferases, Rab-like GTPase activators and Myotubularins (PH-GRAM) domain, repeat 1; TBC1D8 may act as a GTPase-activating protein for Rab family protein(s). TBC1D8 contains an N-terminal PH-GRAM domain and a C-terminal Rab-GTPase-TBC (Tre-2, BUB2p, and Cdc16p) domain. This cd contains the first repeat of the PH-GRAM domain. The GRAM domain is found in glucosyltransferases, myotubularins and other putative membrane-associated proteins. The GRAM domain is part of a larger motif with a pleckstrin homology (PH) domain fold.


Pssm-ID: 270156  Cd Length: 99  Bit Score: 40.61  E-value: 2.24e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1831506055  90 DQFLASYSCA-YQREILAQGRMFISQFNVCFYANIIGWETTLVIPMKEISLVKKMKAAFIfPNSIQIERDTKEKYfFASF 168
Cdd:cd13349     2 EKLVTYYSCCcWKGRVPRQGWLYLSINHLCFYSFFLGKELKLLIPWVDVQKLERTSNVFM-TDTIRVTTQNKERD-FSMF 79

                          90
                  ....*....|
gi 1831506055 169 INRDKSYQVL 178
Cdd:cd13349    80 LNIDEVFRIM 89
PH-GRAM2_TCB1D8_TCB1D9_family cd13218
TCB1D8 and TCB1D9 family Pleckstrin Homology-Glucosyltransferases, Rab-like GTPase activators ...
 108-172 2.45e-04

TCB1D8 and TCB1D9 family Pleckstrin Homology-Glucosyltransferases, Rab-like GTPase activators and Myotubularins (PH-GRAM) domain, repeat 2; TBC1D8, TBC1D8B, TBC1D9 and TBC1D9B may act as a GTPase-activating proteins for Rab family protein(s). They all contain an N-terminal PH-GRAM domain and a C-terminal Rab-GTPase-TBC (Tre-2, BUB2p, and Cdc16p) domain. This cd contains the second repeat of the PH-GRAM domain. The GRAM domain is found in glucosyltransferases, myotubularins and other putative membrane-associated proteins. The GRAM domain is part of a larger motif with a pleckstrin homology (PH) domain fold.


Pssm-ID: 275405  Cd Length: 96  Bit Score: 40.65  E-value: 2.45e-04
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 1831506055 108 GRMFISQFNVCFyANIIGWETTLVIPMKEISLVKKMKAAFIFPNSIQIERDTKEKYFFASFINRD 172
Cdd:cd13218    23 GKMFISENYICF-ASKEGNLCSVIIPLREVLAIEKTNDSSVLPKPVIISIKGKMAFRFSELKDRD 86
PH-GRAM2_TBC1D8 cd13353
TBC1 domain family member 8 (TBC1D8; also called Vascular Rab-GAP/TBC-containing protein) ...
 108-172 5.24e-03

TBC1 domain family member 8 (TBC1D8; also called Vascular Rab-GAP/TBC-containing protein) Pleckstrin Homology-Glucosyltransferases, Rab-like GTPase activators and Myotubularins (PH-GRAM) domain, repeat 2; TBC1D8 may act as a GTPase-activating protein for Rab family protein(s). TBC1D8 contains two N-terminal PH-GRAM domain and a C-terminal Rab-GTPase-TBC (Tre-2, BUB2p, and Cdc16p) domain. This cd contains the second repeat of the PH-GRAM domain. The GRAM domain is found in glucosyltransferases, myotubularins and other putative membrane-associated proteins. The GRAM domain is part of a larger motif with a pleckstrin homology (PH) domain fold.


Pssm-ID: 270160  Cd Length: 96  Bit Score: 36.74  E-value: 5.24e-03
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 1831506055 108 GRMFISQFNVCFYANIIGwETTLVIPMKEISLVKKMKAAFIFPNSIQIERDTKEKYFFASFINRD 172
Cdd:cd13353    23 GRMYTSDSYICFASKEDG-SCNVILPLREVVSIEKMEDTSLLPNPIIVSIRSKMAFQFIELKDRD 86
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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