NCBI Home Page NCBI Site Search page NCBI Guide that lists and describes the NCBI resources
Conserved domains on  [gi|1176461141|ref|NP_001337162|]
View 

probable tRNA (uracil-O(2)-)-methyltransferase isoform 3 [Homo sapiens]

Protein Classification

class I SAM-dependent methyltransferase( domain architecture ID 4222)

class I SAM-dependent methyltransferase that catalyzes the methylation of one or more specific substrates using S-adenosyl-L-methionine (SAM or AdoMet) as the methyl donor; similar to tRNA (uracil-O(2)-)-methyltransferase that catalyzes the formation of O(2)-methyluracil at position 44 (Um44) in tRNA(Ser)

CATH:  2.20.25.110
EC:  2.1.1.-
Gene Ontology:  GO:0008168|GO:1904047
PubMed:  12504684|12826405
SCOP:  3000118

Graphical summary

 Zoom to residue level

show extra options »

Show site features     Horizontal zoom: ×

List of domain hits

 Name Accession Description Interval E-value
AdoMet_MTase super family cl06678
Predicted AdoMet-dependent methyltransferase; Proteins in this family have been predicted to ...
 55-166 9.89e-35

Predicted AdoMet-dependent methyltransferase; Proteins in this family have been predicted to function as AdoMet-dependent methyltransferases.


The actual alignment was detected with superfamily member pfam07757:

Pssm-ID: 462255  Cd Length: 115  Bit Score: 126.30  E-value: 9.89e-35
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1176461141  55 LISIMKYSKAYQELKEKY-KEMVKVWP---EVTDPEKFVYEDVAIAAYLLILWEEERAERRLTarqSFVDLGCGNGLLVH 130
Cdd:pfam07757   1 LVEREKFNDLYKELKDKHaKQLLDHWKtaqEKTDPGKFIFEDLAIAAFLIELWKQTQSENKEF---AFADLGCGNGLLVH 77

                          90       100       110
                  ....*....|....*....|....*....|....*...
gi 1176461141 131 ILSSEGHPGRGIDVRRRKIWDMYGPQTQ--LEEDAITP 166
Cdd:pfam07757  78 LLLAEGYKGYGFDARKRKLWELYPLDTQrkLIEKALEP 115
 
Name Accession Description Interval E-value
AdoMet_MTase pfam07757
Predicted AdoMet-dependent methyltransferase; Proteins in this family have been predicted to ...
 55-166 9.89e-35

Predicted AdoMet-dependent methyltransferase; Proteins in this family have been predicted to function as AdoMet-dependent methyltransferases.


Pssm-ID: 462255  Cd Length: 115  Bit Score: 126.30  E-value: 9.89e-35
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1176461141  55 LISIMKYSKAYQELKEKY-KEMVKVWP---EVTDPEKFVYEDVAIAAYLLILWEEERAERRLTarqSFVDLGCGNGLLVH 130
Cdd:pfam07757   1 LVEREKFNDLYKELKDKHaKQLLDHWKtaqEKTDPGKFIFEDLAIAAFLIELWKQTQSENKEF---AFADLGCGNGLLVH 77

                          90       100       110
                  ....*....|....*....|....*....|....*...
gi 1176461141 131 ILSSEGHPGRGIDVRRRKIWDMYGPQTQ--LEEDAITP 166
Cdd:pfam07757  78 LLLAEGYKGYGFDARKRKLWELYPLDTQrkLIEKALEP 115
 
Name Accession Description Interval E-value
AdoMet_MTase pfam07757
Predicted AdoMet-dependent methyltransferase; Proteins in this family have been predicted to ...
 55-166 9.89e-35

Predicted AdoMet-dependent methyltransferase; Proteins in this family have been predicted to function as AdoMet-dependent methyltransferases.


Pssm-ID: 462255  Cd Length: 115  Bit Score: 126.30  E-value: 9.89e-35
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1176461141  55 LISIMKYSKAYQELKEKY-KEMVKVWP---EVTDPEKFVYEDVAIAAYLLILWEEERAERRLTarqSFVDLGCGNGLLVH 130
Cdd:pfam07757   1 LVEREKFNDLYKELKDKHaKQLLDHWKtaqEKTDPGKFIFEDLAIAAFLIELWKQTQSENKEF---AFADLGCGNGLLVH 77

                          90       100       110
                  ....*....|....*....|....*....|....*...
gi 1176461141 131 ILSSEGHPGRGIDVRRRKIWDMYGPQTQ--LEEDAITP 166
Cdd:pfam07757  78 LLLAEGYKGYGFDARKRKLWELYPLDTQrkLIEKALEP 115
Methyltransf_32 pfam13679
Methyltransferase domain; This family appears to be a methyltransferase domain.
 119-209 3.53e-04

Methyltransferase domain; This family appears to be a methyltransferase domain.


Pssm-ID: 379330 [Multi-domain]  Cd Length: 138  Bit Score: 40.63  E-value: 3.53e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1176461141 119 VDLGCGNGLLVHILSSEGHPGR--GIDVRR------RKIWDMYGPQTQLEEDAITPNDKTLF---PDVDWLIGNHS-DEL 186
Cdd:pfam13679  30 VDHGAGKGYLGFILYYLKYGVRvyGIDTRAelvekaNALAQKLGFNKRMSFLEGTIAGSTPVelpDRVDVVTALHAcDTA 109

                          90       100
                  ....*....|....*....|....
gi 1176461141 187 TPWIPVIAARSsyNCRFFVL-PCC 209
Cdd:pfam13679 110 TDDALRFALAK--QARAIVLvPCC 131
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
Help | Disclaimer | Write to the Help Desk
NCBI | NLM | NIH