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Conserved domains on  [gi|1063742720|ref|NP_001330603|]
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auxin response factor 2 [Arabidopsis thaliana]

Protein Classification

auxin response factor( domain architecture ID 10492348)

auxin response factor (ARF) can function as a transcriptional activator or repressor to regulate the expression of auxin response genes by specifically binding to auxin response elements (AuxREs) during plant development

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
Auxin_resp pfam06507
Auxin response factor; A conserved region of auxin-responsive transcription factors.
 290-372 3.77e-48

Auxin response factor; A conserved region of auxin-responsive transcription factors.


:

Pssm-ID: 428981  Cd Length: 83  Bit Score: 165.02  E-value: 3.77e-48
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063742720 290 AWHAISTGTMFTVYYKPRTSPSEFIVPFDQYMESVKNNYSIGMRFKMRFEGEEAPEQRFTGTIVGIEESDPTRWPKSKWR 369
Cdd:pfam06507   1 AAHAAATGSPFTVVYYPRASPSEFVVPYNKVLKAMNNPWSVGMRFKMRFETEDSSERRYSGTISGVGDADPVRWPGSPWR 80


                  ...
gi 1063742720 370 SLK 372
Cdd:pfam06507  81 SLQ 83
B3 pfam02362
B3 DNA binding domain; This is a family of plant transcription factors with various roles in ...
 164-265 2.37e-25

B3 DNA binding domain; This is a family of plant transcription factors with various roles in development, the aligned region corresponds the B3 DNA binding domain as described in this domain is found in VP1/AB13 transcription factors. Some proteins also have a second AP2 DNA binding domain pfam00847 such as RAV1. DNA binding activity was demonstrated by.


:

Pssm-ID: 396783 [Multi-domain]  Cd Length: 101  Bit Score: 100.91  E-value: 2.37e-25
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063742720 164 FCKTLTASDTSTHGGFSVLRRHADECLPpldmSRQPPTQELVAKDLHANEWRFRHIFRGQPRRHLLQSGWSVFVSSKRLV 243
Cdd:pfam02362   1 FFKVLVTSDVLSSGRLVIPKKFARENSG----LPKRLSKEITLLDEHGKSWTVKLRYRKKSGRYVLTGGWKEFVKANGLK 76

                          90       100
                  ....*....|....*....|....
gi 1063742720 244 AGDAFIF--LRGENGELRVGVRRA 265
Cdd:pfam02362  77 AGDFLTFklVGNSGFTPVVGIFRK 100
AUX_IAA super family cl03528
AUX/IAA family; Transcription of the AUX/IAA family of genes is rapidly induced by the plant ...
 716-812 8.34e-08

AUX/IAA family; Transcription of the AUX/IAA family of genes is rapidly induced by the plant hormone auxin. Some members of this family are longer and contain an N terminal DNA binding domain. The function of this region is uncertain.


The actual alignment was detected with superfamily member pfam02309:

Pssm-ID: 460529  Cd Length: 188  Bit Score: 53.04  E-value: 8.34e-08
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063742720 716 QTNNPHPKDAQTKTNSS-RSCT--KVHKQGIALGRSVDLSKFQNYEELVAELDRLF------------EFNGELMAPKK- 779
Cdd:pfam02309  57 SSSKNSKEEAEGKAGEGcKKALfvKVSMDGAPYLRKVDLKAYDSYQELSSALEKMFscftigqcgshgMPGRDGLNESKl 136

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|
gi 1063742720 780 -------DWLIVYTDEENDMMLVGDDPWQEFCCMVRKIFI 812
Cdd:pfam02309 137 sdlldgsEYVLTYEDKDGDWMLVGDVPWEMFVDSCKRLRI 176
 
Name Accession Description Interval E-value
Auxin_resp pfam06507
Auxin response factor; A conserved region of auxin-responsive transcription factors.
 290-372 3.77e-48

Auxin response factor; A conserved region of auxin-responsive transcription factors.


Pssm-ID: 428981  Cd Length: 83  Bit Score: 165.02  E-value: 3.77e-48
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063742720 290 AWHAISTGTMFTVYYKPRTSPSEFIVPFDQYMESVKNNYSIGMRFKMRFEGEEAPEQRFTGTIVGIEESDPTRWPKSKWR 369
Cdd:pfam06507   1 AAHAAATGSPFTVVYYPRASPSEFVVPYNKVLKAMNNPWSVGMRFKMRFETEDSSERRYSGTISGVGDADPVRWPGSPWR 80


                  ...
gi 1063742720 370 SLK 372
Cdd:pfam06507  81 SLQ 83
B3 pfam02362
B3 DNA binding domain; This is a family of plant transcription factors with various roles in ...
 164-265 2.37e-25

B3 DNA binding domain; This is a family of plant transcription factors with various roles in development, the aligned region corresponds the B3 DNA binding domain as described in this domain is found in VP1/AB13 transcription factors. Some proteins also have a second AP2 DNA binding domain pfam00847 such as RAV1. DNA binding activity was demonstrated by.


Pssm-ID: 396783 [Multi-domain]  Cd Length: 101  Bit Score: 100.91  E-value: 2.37e-25
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063742720 164 FCKTLTASDTSTHGGFSVLRRHADECLPpldmSRQPPTQELVAKDLHANEWRFRHIFRGQPRRHLLQSGWSVFVSSKRLV 243
Cdd:pfam02362   1 FFKVLVTSDVLSSGRLVIPKKFARENSG----LPKRLSKEITLLDEHGKSWTVKLRYRKKSGRYVLTGGWKEFVKANGLK 76

                          90       100
                  ....*....|....*....|....
gi 1063742720 244 AGDAFIF--LRGENGELRVGVRRA 265
Cdd:pfam02362  77 AGDFLTFklVGNSGFTPVVGIFRK 100
B3 smart01019
B3 DNA binding domain; Two DNA binding proteins, RAV1 and RAV2 from Arabidopsis thaliana ...
 164-265 5.27e-25

B3 DNA binding domain; Two DNA binding proteins, RAV1 and RAV2 from Arabidopsis thaliana contain two distinct amino acid sequence domains found only in higher plant species. The N-terminal regions of RAV1 and RAV2 are homologous to the AP2 DNA-binding domain (see ) present in a family of transcription factors, while the C-terminal region exhibits homology to the highly conserved C-terminal domain, designated B3, of VP1/ABI3 transcription factors. The AP2 and B3-like domains of RAV1 bind autonomously to the CAACA and CACCTG motifs, respectively, and together achieve a high affinity and specificity of binding. It has been suggested that the AP2 and B3-like domains of RAV1 are connected by a highly flexible structure enabling the two domains to bind to the CAACA and CACCTG motifs in various spacings and orientations.


Pssm-ID: 214977  Cd Length: 96  Bit Score: 99.68  E-value: 5.27e-25
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063742720  164 FCKTLTASDTSTHGGFSVLRRHADECLPPLDMsrqpptQELVAKDLHANEWRFRHIFRGQPRRHLLQSGWSVFVSSKRLV 243
Cdd:smart01019   1 FFKTLTTSDVSKHGRLVLPKQFAESNGPPLKP------KEITLLDPDGKSWKVKLKYRGSSGMFLLTGGWKEFVKANGLK 74

                           90       100
                   ....*....|....*....|..
gi 1063742720  244 AGDAFIFLRGENGELRVGVRRA 265
Cdd:smart01019  75 AGDSLTFELDGNGEFVVSIFRK 96
B3_DNA cd10017
Plant-specific B3-DNA binding domain; The plant-specific B3 DNA binding domain superfamily ...
 162-264 1.32e-20

Plant-specific B3-DNA binding domain; The plant-specific B3 DNA binding domain superfamily includes the well-characterized auxin response factor (ARF) and the LAV (Leafy cotyledon2 [LEC2]-Abscisic acid insensitive3 [ABI3]-VAL) families, as well as the RAV (Related to ABI3 and VP1) and REM (REproductive Meristem) families. LEC2 and ABI3 have been shown to be involved in seed development, while other members of the LAV family seem to have a more general role, being expressed in many organs during plant development. Members of the ARF family bind to the auxin response element and depending on presence of an activation or repression domain, they activate or repress transcription. RAV and REM families are less studied B3 protein famillies.


Pssm-ID: 197383  Cd Length: 98  Bit Score: 87.38  E-value: 1.32e-20
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063742720 162 HSFCKTLTASDTSTHGGFSVLRRHADECLPPldmsrqPPTQELVAKDLHANEWRFRHIFRGQPRRHLLQSGWSVFVSSKR 241
Cdd:cd10017     1 PLFFKVLTPSDVSSLGRLVLPKKFAKAHLPP------KEGVEVTLEDPDGKKWTVKLKKRKNSGRMVLSGGWKEFVRANG 74

                          90       100
                  ....*....|....*....|....
gi 1063742720 242 LVAGDAFIF-LRGENGELRVGVRR 264
Cdd:cd10017    75 LKEGDFLVFeLDGRNSKFKVRIFR 98
AUX_IAA pfam02309
AUX/IAA family; Transcription of the AUX/IAA family of genes is rapidly induced by the plant ...
 716-812 8.34e-08

AUX/IAA family; Transcription of the AUX/IAA family of genes is rapidly induced by the plant hormone auxin. Some members of this family are longer and contain an N terminal DNA binding domain. The function of this region is uncertain.


Pssm-ID: 460529  Cd Length: 188  Bit Score: 53.04  E-value: 8.34e-08
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063742720 716 QTNNPHPKDAQTKTNSS-RSCT--KVHKQGIALGRSVDLSKFQNYEELVAELDRLF------------EFNGELMAPKK- 779
Cdd:pfam02309  57 SSSKNSKEEAEGKAGEGcKKALfvKVSMDGAPYLRKVDLKAYDSYQELSSALEKMFscftigqcgshgMPGRDGLNESKl 136

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|
gi 1063742720 780 -------DWLIVYTDEENDMMLVGDDPWQEFCCMVRKIFI 812
Cdd:pfam02309 137 sdlldgsEYVLTYEDKDGDWMLVGDVPWEMFVDSCKRLRI 176
 
Name Accession Description Interval E-value
Auxin_resp pfam06507
Auxin response factor; A conserved region of auxin-responsive transcription factors.
 290-372 3.77e-48

Auxin response factor; A conserved region of auxin-responsive transcription factors.


Pssm-ID: 428981  Cd Length: 83  Bit Score: 165.02  E-value: 3.77e-48
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063742720 290 AWHAISTGTMFTVYYKPRTSPSEFIVPFDQYMESVKNNYSIGMRFKMRFEGEEAPEQRFTGTIVGIEESDPTRWPKSKWR 369
Cdd:pfam06507   1 AAHAAATGSPFTVVYYPRASPSEFVVPYNKVLKAMNNPWSVGMRFKMRFETEDSSERRYSGTISGVGDADPVRWPGSPWR 80


                  ...
gi 1063742720 370 SLK 372
Cdd:pfam06507  81 SLQ 83
B3 pfam02362
B3 DNA binding domain; This is a family of plant transcription factors with various roles in ...
 164-265 2.37e-25

B3 DNA binding domain; This is a family of plant transcription factors with various roles in development, the aligned region corresponds the B3 DNA binding domain as described in this domain is found in VP1/AB13 transcription factors. Some proteins also have a second AP2 DNA binding domain pfam00847 such as RAV1. DNA binding activity was demonstrated by.


Pssm-ID: 396783 [Multi-domain]  Cd Length: 101  Bit Score: 100.91  E-value: 2.37e-25
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063742720 164 FCKTLTASDTSTHGGFSVLRRHADECLPpldmSRQPPTQELVAKDLHANEWRFRHIFRGQPRRHLLQSGWSVFVSSKRLV 243
Cdd:pfam02362   1 FFKVLVTSDVLSSGRLVIPKKFARENSG----LPKRLSKEITLLDEHGKSWTVKLRYRKKSGRYVLTGGWKEFVKANGLK 76

                          90       100
                  ....*....|....*....|....
gi 1063742720 244 AGDAFIF--LRGENGELRVGVRRA 265
Cdd:pfam02362  77 AGDFLTFklVGNSGFTPVVGIFRK 100
B3 smart01019
B3 DNA binding domain; Two DNA binding proteins, RAV1 and RAV2 from Arabidopsis thaliana ...
 164-265 5.27e-25

B3 DNA binding domain; Two DNA binding proteins, RAV1 and RAV2 from Arabidopsis thaliana contain two distinct amino acid sequence domains found only in higher plant species. The N-terminal regions of RAV1 and RAV2 are homologous to the AP2 DNA-binding domain (see ) present in a family of transcription factors, while the C-terminal region exhibits homology to the highly conserved C-terminal domain, designated B3, of VP1/ABI3 transcription factors. The AP2 and B3-like domains of RAV1 bind autonomously to the CAACA and CACCTG motifs, respectively, and together achieve a high affinity and specificity of binding. It has been suggested that the AP2 and B3-like domains of RAV1 are connected by a highly flexible structure enabling the two domains to bind to the CAACA and CACCTG motifs in various spacings and orientations.


Pssm-ID: 214977  Cd Length: 96  Bit Score: 99.68  E-value: 5.27e-25
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063742720  164 FCKTLTASDTSTHGGFSVLRRHADECLPPLDMsrqpptQELVAKDLHANEWRFRHIFRGQPRRHLLQSGWSVFVSSKRLV 243
Cdd:smart01019   1 FFKTLTTSDVSKHGRLVLPKQFAESNGPPLKP------KEITLLDPDGKSWKVKLKYRGSSGMFLLTGGWKEFVKANGLK 74

                           90       100
                   ....*....|....*....|..
gi 1063742720  244 AGDAFIFLRGENGELRVGVRRA 265
Cdd:smart01019  75 AGDSLTFELDGNGEFVVSIFRK 96
B3_DNA cd10017
Plant-specific B3-DNA binding domain; The plant-specific B3 DNA binding domain superfamily ...
 162-264 1.32e-20

Plant-specific B3-DNA binding domain; The plant-specific B3 DNA binding domain superfamily includes the well-characterized auxin response factor (ARF) and the LAV (Leafy cotyledon2 [LEC2]-Abscisic acid insensitive3 [ABI3]-VAL) families, as well as the RAV (Related to ABI3 and VP1) and REM (REproductive Meristem) families. LEC2 and ABI3 have been shown to be involved in seed development, while other members of the LAV family seem to have a more general role, being expressed in many organs during plant development. Members of the ARF family bind to the auxin response element and depending on presence of an activation or repression domain, they activate or repress transcription. RAV and REM families are less studied B3 protein famillies.


Pssm-ID: 197383  Cd Length: 98  Bit Score: 87.38  E-value: 1.32e-20
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063742720 162 HSFCKTLTASDTSTHGGFSVLRRHADECLPPldmsrqPPTQELVAKDLHANEWRFRHIFRGQPRRHLLQSGWSVFVSSKR 241
Cdd:cd10017     1 PLFFKVLTPSDVSSLGRLVLPKKFAKAHLPP------KEGVEVTLEDPDGKKWTVKLKKRKNSGRMVLSGGWKEFVRANG 74

                          90       100
                  ....*....|....*....|....
gi 1063742720 242 LVAGDAFIF-LRGENGELRVGVRR 264
Cdd:cd10017    75 LKEGDFLVFeLDGRNSKFKVRIFR 98
AUX_IAA pfam02309
AUX/IAA family; Transcription of the AUX/IAA family of genes is rapidly induced by the plant ...
 716-812 8.34e-08

AUX/IAA family; Transcription of the AUX/IAA family of genes is rapidly induced by the plant hormone auxin. Some members of this family are longer and contain an N terminal DNA binding domain. The function of this region is uncertain.


Pssm-ID: 460529  Cd Length: 188  Bit Score: 53.04  E-value: 8.34e-08
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063742720 716 QTNNPHPKDAQTKTNSS-RSCT--KVHKQGIALGRSVDLSKFQNYEELVAELDRLF------------EFNGELMAPKK- 779
Cdd:pfam02309  57 SSSKNSKEEAEGKAGEGcKKALfvKVSMDGAPYLRKVDLKAYDSYQELSSALEKMFscftigqcgshgMPGRDGLNESKl 136

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|
gi 1063742720 780 -------DWLIVYTDEENDMMLVGDDPWQEFCCMVRKIFI 812
Cdd:pfam02309 137 sdlldgsEYVLTYEDKDGDWMLVGDVPWEMFVDSCKRLRI 176
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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