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Conserved domains on  [gi|1063723944|ref|NP_001328972|]
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fatty acid hydroxylase 2 [Arabidopsis thaliana]

Protein Classification

sterol desaturase family protein; fatty acid 2-hydroxylase( domain architecture ID 10010789)

sterol desaturase family protein is an oxidoreductase, similar to C-5 sterol desaturase, which catalyzes a dehydrogenation reaction to introduce a C5-6 double bond into substrates such as lathosterol and the B ring of ergosterol| fatty acid 2-hydroxylase is an integral membrane involved in processes such as cholesterol biosynthesis or biosynthesis of plant cuticular wax. It catalyzes the hydroxylation of free fatty acids at the C-2 position to produce 2-hydroxy fatty acids, which are building blocks of sphingolipids and glycosphingolipids, for example, and contains an N-terminal cytochrome b5-like heme/steroid binding domain

Graphical summary

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List of domain hits

Name Accession Description Interval E-value
PLN02434 PLN02434
fatty acid hydroxylase
1-237 2.03e-156

fatty acid hydroxylase


:

Pssm-ID: 178053 [Multi-domain]  Cd Length: 237  Bit Score: 433.30  E-value: 2.03e-156
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063723944   1 MVAERYTVDLNKPLVFQVGHLGEEYQEWIHQPIVCVEGPRFFESDFWEFLTRTVWWAIPTIWLPVVCYVLSISASKGLTF 80
Cdd:PLN02434    1 MVAQKFTVDLNKPLVFQVGHLGEDYEEWVHQPIVSKEGPRFFESDVLEFLTRTVWWAVPLIWLPVVCWCLVKSVRMGLPL 80
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063723944  81 PQIGLIVAFGVLTWTLLEYTLHRFLFHIQTKSYWANTAHYLLHGCHHKHPQDGLRLVFPPTATAILLVPLWKLLHLLATP 160
Cdd:PLN02434   81 SAVVLMVAFGVFIWTLLEYILHRFLFHIKTKSYWGNTAHYLLHGCHHKHPMDGLRLVFPPAATAILCVPFWNLIALFATP 160
                         170       180       190       200       210       220       230
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 1063723944 161 ATAPAILGGILFGYVMYDITHYYLHHGQPKEPTFKHLKKYHLNHHFRIQDKGYGITSSLWDKVFGTLPGIKAAAKKS 237
Cdd:PLN02434  161 ATAPALFGGGLLGYVMYDCTHYFLHHGQPSTDVLRNLKKYHLNHHFRDQDKGFGITSSLWDRVFGTLPPSKAAKKKR 237
 
Name Accession Description Interval E-value
PLN02434 PLN02434
fatty acid hydroxylase
1-237 2.03e-156

fatty acid hydroxylase


Pssm-ID: 178053 [Multi-domain]  Cd Length: 237  Bit Score: 433.30  E-value: 2.03e-156
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063723944   1 MVAERYTVDLNKPLVFQVGHLGEEYQEWIHQPIVCVEGPRFFESDFWEFLTRTVWWAIPTIWLPVVCYVLSISASKGLTF 80
Cdd:PLN02434    1 MVAQKFTVDLNKPLVFQVGHLGEDYEEWVHQPIVSKEGPRFFESDVLEFLTRTVWWAVPLIWLPVVCWCLVKSVRMGLPL 80
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063723944  81 PQIGLIVAFGVLTWTLLEYTLHRFLFHIQTKSYWANTAHYLLHGCHHKHPQDGLRLVFPPTATAILLVPLWKLLHLLATP 160
Cdd:PLN02434   81 SAVVLMVAFGVFIWTLLEYILHRFLFHIKTKSYWGNTAHYLLHGCHHKHPMDGLRLVFPPAATAILCVPFWNLIALFATP 160
                         170       180       190       200       210       220       230
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 1063723944 161 ATAPAILGGILFGYVMYDITHYYLHHGQPKEPTFKHLKKYHLNHHFRIQDKGYGITSSLWDKVFGTLPGIKAAAKKS 237
Cdd:PLN02434  161 ATAPALFGGGLLGYVMYDCTHYFLHHGQPSTDVLRNLKKYHLNHHFRDQDKGFGITSSLWDRVFGTLPPSKAAKKKR 237
FA_hydroxylase pfam04116
Fatty acid hydroxylase; This entry includes fatty acid and carotene hydroxylases and sterol ...
87-226 7.28e-15

Fatty acid hydroxylase; This entry includes fatty acid and carotene hydroxylases and sterol desaturases. Beta-carotene hydroxylase is involved in zeaxanthin synthesis by hydroxylating beta-carotene, but the enzyme may be involved in other pathways. This family includes C-5 sterol desaturase and C-4 sterol methyl oxidase. Members of this family are involved in cholesterol biosynthesis and biosynthesis a plant cuticular wax. These enzymes contain two copies of a HXHH motif which coordinate two irons at the catalytic centre. Members of this family are ER integral membrane proteins that share a novel mushroom-shaped fold consisting of four transmembrane (TM1-TM4) helices that anchor them to the membrane capped by a cytosolic domain containing the unique histidine- coordinating di metal centre.


Pssm-ID: 397991  Cd Length: 134  Bit Score: 69.00  E-value: 7.28e-15
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063723944  87 VAFGVLTWTLLEYTLHRfLFHIQTKSYwantahYLLHGCHHKHPQDGLRLVFPPTATAILLVPLWKLLHLLATPATAPAI 166
Cdd:pfam04116   1 VLLGLLLFDFLFYWVHR-LLHRLPWLW------RRFHKVHHSSEAPNALTALRFHPLEALLFALLVLLPLLLLGLPLLAF 73
                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|.
gi 1063723944 167 LGGILFGYVMYDITHYYLHHG-QPKEPTFKHLKKYHLNHHFRIQDKGYGITSSLWDKVFGT 226
Cdd:pfam04116  74 LLGLLLGTLWYLFIHSGLLFPlPWLLKRLLGTPRFHRLHHSKNEEYNFGVTFPLWDRLFGT 134
ERG3 COG3000
Sterol desaturase/sphingolipid hydroxylase, fatty acid hydroxylase superfamily [Lipid ...
87-226 5.49e-05

Sterol desaturase/sphingolipid hydroxylase, fatty acid hydroxylase superfamily [Lipid transport and metabolism];


Pssm-ID: 442238  Cd Length: 158  Bit Score: 42.23  E-value: 5.49e-05
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063723944  87 VAFGVLTWTLLEYTLHRFLFHIqtKSYWAntahylLHGCHHKHPQDGLRLVFPPTATAILLVPLWKLLHLLATPATAPAI 166
Cdd:COG3000     2 FLLAFLLLDFVYYWLHRLSHRV--PLLWR------FHAVHHSSERMNLLTALRFHPLEILLSALLFLLPLALLGFPPEAV 73
                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 1063723944 167 LGGILFGYVMYDITHyyLHHGQPKEPTFKHLK---KYHLNHHFRIQ---DKGYGITSSLWDKVFGT 226
Cdd:COG3000    74 LLYLALNLLYQFFNH--SNIRLPLDGPLEYVFvtpSHHRVHHSRNPeylDKNYGGILSIWDRLFGT 137
 
Name Accession Description Interval E-value
PLN02434 PLN02434
fatty acid hydroxylase
1-237 2.03e-156

fatty acid hydroxylase


Pssm-ID: 178053 [Multi-domain]  Cd Length: 237  Bit Score: 433.30  E-value: 2.03e-156
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063723944   1 MVAERYTVDLNKPLVFQVGHLGEEYQEWIHQPIVCVEGPRFFESDFWEFLTRTVWWAIPTIWLPVVCYVLSISASKGLTF 80
Cdd:PLN02434    1 MVAQKFTVDLNKPLVFQVGHLGEDYEEWVHQPIVSKEGPRFFESDVLEFLTRTVWWAVPLIWLPVVCWCLVKSVRMGLPL 80
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063723944  81 PQIGLIVAFGVLTWTLLEYTLHRFLFHIQTKSYWANTAHYLLHGCHHKHPQDGLRLVFPPTATAILLVPLWKLLHLLATP 160
Cdd:PLN02434   81 SAVVLMVAFGVFIWTLLEYILHRFLFHIKTKSYWGNTAHYLLHGCHHKHPMDGLRLVFPPAATAILCVPFWNLIALFATP 160
                         170       180       190       200       210       220       230
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 1063723944 161 ATAPAILGGILFGYVMYDITHYYLHHGQPKEPTFKHLKKYHLNHHFRIQDKGYGITSSLWDKVFGTLPGIKAAAKKS 237
Cdd:PLN02434  161 ATAPALFGGGLLGYVMYDCTHYFLHHGQPSTDVLRNLKKYHLNHHFRDQDKGFGITSSLWDRVFGTLPPSKAAKKKR 237
FA_hydroxylase pfam04116
Fatty acid hydroxylase; This entry includes fatty acid and carotene hydroxylases and sterol ...
87-226 7.28e-15

Fatty acid hydroxylase; This entry includes fatty acid and carotene hydroxylases and sterol desaturases. Beta-carotene hydroxylase is involved in zeaxanthin synthesis by hydroxylating beta-carotene, but the enzyme may be involved in other pathways. This family includes C-5 sterol desaturase and C-4 sterol methyl oxidase. Members of this family are involved in cholesterol biosynthesis and biosynthesis a plant cuticular wax. These enzymes contain two copies of a HXHH motif which coordinate two irons at the catalytic centre. Members of this family are ER integral membrane proteins that share a novel mushroom-shaped fold consisting of four transmembrane (TM1-TM4) helices that anchor them to the membrane capped by a cytosolic domain containing the unique histidine- coordinating di metal centre.


Pssm-ID: 397991  Cd Length: 134  Bit Score: 69.00  E-value: 7.28e-15
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063723944  87 VAFGVLTWTLLEYTLHRfLFHIQTKSYwantahYLLHGCHHKHPQDGLRLVFPPTATAILLVPLWKLLHLLATPATAPAI 166
Cdd:pfam04116   1 VLLGLLLFDFLFYWVHR-LLHRLPWLW------RRFHKVHHSSEAPNALTALRFHPLEALLFALLVLLPLLLLGLPLLAF 73
                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|.
gi 1063723944 167 LGGILFGYVMYDITHYYLHHG-QPKEPTFKHLKKYHLNHHFRIQDKGYGITSSLWDKVFGT 226
Cdd:pfam04116  74 LLGLLLGTLWYLFIHSGLLFPlPWLLKRLLGTPRFHRLHHSKNEEYNFGVTFPLWDRLFGT 134
ERG3 COG3000
Sterol desaturase/sphingolipid hydroxylase, fatty acid hydroxylase superfamily [Lipid ...
87-226 5.49e-05

Sterol desaturase/sphingolipid hydroxylase, fatty acid hydroxylase superfamily [Lipid transport and metabolism];


Pssm-ID: 442238  Cd Length: 158  Bit Score: 42.23  E-value: 5.49e-05
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063723944  87 VAFGVLTWTLLEYTLHRFLFHIqtKSYWAntahylLHGCHHKHPQDGLRLVFPPTATAILLVPLWKLLHLLATPATAPAI 166
Cdd:COG3000     2 FLLAFLLLDFVYYWLHRLSHRV--PLLWR------FHAVHHSSERMNLLTALRFHPLEILLSALLFLLPLALLGFPPEAV 73
                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 1063723944 167 LGGILFGYVMYDITHyyLHHGQPKEPTFKHLK---KYHLNHHFRIQ---DKGYGITSSLWDKVFGT 226
Cdd:COG3000    74 LLYLALNLLYQFFNH--SNIRLPLDGPLEYVFvtpSHHRVHHSRNPeylDKNYGGILSIWDRLFGT 137
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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