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Conserved domains on  [gi|1063709997|ref|NP_001326713|]
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glucan synthase-like 10 [Arabidopsis thaliana]

Protein Classification

FKS1_dom1 and Glucan_synthase domain-containing protein( domain architecture ID 10626192)

FKS1_dom1 and Glucan_synthase domain-containing protein

Graphical summary

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List of domain hits

Name Accession Description Interval E-value
Glucan_synthase super family cl15642
1,3-beta-glucan synthase component; This family consists of various 1,3-beta-glucan synthase ...
1025-1757 0e+00

1,3-beta-glucan synthase component; This family consists of various 1,3-beta-glucan synthase components including Gls1, Gls2 and Gls3 from yeast. 1,3-beta-glucan synthase EC:2.4.1.34 also known as callose synthase catalyzes the formation of a beta-1,3-glucan polymer that is a major component of the fungal cell wall. The reaction catalyzed is:- UDP-glucose + {(1,3)-beta-D-glucosyl}(N) <=> UDP + {(1,3)-beta-D-glucosyl}(N+1).


The actual alignment was detected with superfamily member pfam02364:

Pssm-ID: 426739  Cd Length: 818  Bit Score: 840.30  E-value: 0e+00
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063709997 1025 VPRNLEARRRLQFFTNSLFMDVPPPKSVRKMLSFSVFTPYYSEVVLYSMAELTKRNEDG--ISILFYLQKIYPDEWKNFL 1102
Cdd:pfam02364    2 FPKNSEAERRISFFAQSLSTPMPEPPPVEKMPTFTVLIPHYSEKILLSLREIIREEEDGsrVTLLEYLKQLHPDEWKNFV 81
                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063709997 1103 ARIGR----DENALEGDLDNERD-----------------------ILELRFWASYRGQTLARTVRGMMYYRKALMLQSY 1155
Cdd:pfam02364   82 EDTKLlaeeDDADDSNSEKDEEDlvkekiddlpfycigfksstpeyTLRTRIWASLRGQTLYRTVSGFMNYSRAIKLLYR 161
                          170       180       190       200       210       220       230       240
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063709997 1156 LERKAGNDATDAEGFELSPEARAQADLKFTYVVTCQIYGRQKEDQKPEAvdiALLMQRNEALRIAYIDvvDSPKEGKSHT 1235
Cdd:pfam02364  162 VENPSLVQLYSGNSEKLERELESMALRKFRLVVSMQRYAKFKAEEDENA---EFLLRAYPDLQIAYLD--EEPDEEGGEP 236
                          250       260       270       280       290       300       310       320
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063709997 1236 EYYSKLVK--ADI---SGKDKEIYSIKLPGDPKLGEGKPENQNHAIVFTRGNAIQTIDMNQDNYFEEALKMRNLLEEFDR 1310
Cdd:pfam02364  237 EYYSVLIDghCEIdqeNGKRKPKYRIRLSGNPILGDGKSDNQNHAIIFYRGEYIQVIDANQDNYLEECLKIRSVLAEFEE 316
                          330       340       350       360       370       380       390       400
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063709997 1311 DH-GIRPP-------------TILGVREHVFTGSVSSLASFMSNQETSFVTLGQRVLAKpLKIRMHYGHPDVFDRVFHIT 1376
Cdd:pfam02364  317 MNlGIRSPyipgiydeeknpvAILGAREYIFSENIGVLGDIAAGKEQTFGTLFARTLAE-IGGKLHYGHPDFLNAIFMTT 395
                          410       420       430       440       450       460       470       480
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063709997 1377 RGGISKASRVINISEDIFAGFNTTLRQGNVTHHEYIQVGKGRDVGLNQIALFEGKVAGGNGEQVLSRDVYRLGQLLDFFR 1456
Cdd:pfam02364  396 RGGVSKAQKGLHLNEDIYAGMNATLRGGRIKHCEYYQCGKGRDLGFGSILNFTTKIGAGMGEQMLSREYYYLGTQLPLDR 475
                          490       500       510       520       530       540       550       560
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063709997 1457 MMSFFFTTVGFYLCTMLTVLTVYIFLYgrAYLALSGV-----------GATIRERAILLDDTALSAALNAQFLFQIGVFT 1525
Cdd:pfam02364  476 FLSFYYAHPGFHLNNMFIMLSVQLFML--LLLNLGALnhesiiceydkDNPITDPERPIGCYNLQPVLNWVSRFVLSIFI 553
                          570       580       590       600       610       620       630       640
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063709997 1526 A-----VPMVLGFILEQGFLQAIVSFITMQFQLCTVFFTFSLGTRTHYFGRTILHGGARYQATGRGFVVKHIKFSENYRL 1600
Cdd:pfam02364  554 VffisfLPLIVQELLERGFLKAVSRFFKHFLSLSPLFEVFVCQIYAHSLLRNLTFGGARYIATGRGFATTRIPFAELYSR 633
                          650       660       670       680       690       700       710       720
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063709997 1601 YSRSHFVKAMEVILLLVVylaygndeAGAVSYILLTVSSWFLAVSWLFAPYLFNPAGFEWQKVVEDFKEWTNWLfYRGGI 1680
Cdd:pfam02364  634 FARSSIYKGIELFLMLLF--------ATTTMWIPALLWFWITVVSLCLAPFLFNPHQFSWLDFFIDYRDFIRWL-SRGNS 704
                          730       740       750       760       770       780       790       800
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063709997 1681 -------------------GVKGAESWEAWWEEELSHIRTLSGRI--METILSL----------RFFIFQYGI------- 1722
Cdd:pfam02364  705 kthenswigyerqsrlritGYKRKLLGDPSEKLSGDVPRASFTNLffSEIILPLivallifiayRFINSQYGVrgpkptn 784
                          810       820       830
                   ....*....|....*....|....*....|....*.
gi 1063709997 1723 -VYKLKLqgsdTSFAVYGWSWVafaMIIVLFKVFTF 1757
Cdd:pfam02364  785 sVYRLAI----VSILPILLNWI---VLLVLFGISCL 813
FKS1_dom1 pfam14288
1,3-beta-glucan synthase subunit FKS1, domain-1; The FKS1_dom1 domain is likely to be the ...
333-448 2.98e-54

1,3-beta-glucan synthase subunit FKS1, domain-1; The FKS1_dom1 domain is likely to be the 'Class I' region just N-terminal to the first set of transmembrane helices that is involved in 1,3-beta-glucan synthesis itself. This family is found on proteins with family Glucan_synthase, pfam02364.


:

Pssm-ID: 464126  Cd Length: 112  Bit Score: 184.73  E-value: 2.98e-54
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063709997  333 KKLLFLSLYFLIWGEAANIRFLPECLCYIFHHMVREMDEILRQQVARPAESCmpvdsrGSDDGVSFLDHVIAPLYGVV-S 411
Cdd:pfam14288    1 RKLLQIALYLLIWGEAANVRFMPECLCYIFHCMAYELNGILDGNVSPMTYSP------YSGPEGSFLDNVITPIYRFIrD 74
                           90       100       110
                   ....*....|....*....|....*....|....*...
gi 1063709997  412 AEAFNNDNGRAPHSAWRNYDDFNEYFWSLHSFE-LGWP 448
Cdd:pfam14288   75 QEYEISKNGEADHSAWIGYDDINQLFWSPECIErLGWP 112
 
Name Accession Description Interval E-value
Glucan_synthase pfam02364
1,3-beta-glucan synthase component; This family consists of various 1,3-beta-glucan synthase ...
1025-1757 0e+00

1,3-beta-glucan synthase component; This family consists of various 1,3-beta-glucan synthase components including Gls1, Gls2 and Gls3 from yeast. 1,3-beta-glucan synthase EC:2.4.1.34 also known as callose synthase catalyzes the formation of a beta-1,3-glucan polymer that is a major component of the fungal cell wall. The reaction catalyzed is:- UDP-glucose + {(1,3)-beta-D-glucosyl}(N) <=> UDP + {(1,3)-beta-D-glucosyl}(N+1).


Pssm-ID: 426739  Cd Length: 818  Bit Score: 840.30  E-value: 0e+00
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063709997 1025 VPRNLEARRRLQFFTNSLFMDVPPPKSVRKMLSFSVFTPYYSEVVLYSMAELTKRNEDG--ISILFYLQKIYPDEWKNFL 1102
Cdd:pfam02364    2 FPKNSEAERRISFFAQSLSTPMPEPPPVEKMPTFTVLIPHYSEKILLSLREIIREEEDGsrVTLLEYLKQLHPDEWKNFV 81
                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063709997 1103 ARIGR----DENALEGDLDNERD-----------------------ILELRFWASYRGQTLARTVRGMMYYRKALMLQSY 1155
Cdd:pfam02364   82 EDTKLlaeeDDADDSNSEKDEEDlvkekiddlpfycigfksstpeyTLRTRIWASLRGQTLYRTVSGFMNYSRAIKLLYR 161
                          170       180       190       200       210       220       230       240
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063709997 1156 LERKAGNDATDAEGFELSPEARAQADLKFTYVVTCQIYGRQKEDQKPEAvdiALLMQRNEALRIAYIDvvDSPKEGKSHT 1235
Cdd:pfam02364  162 VENPSLVQLYSGNSEKLERELESMALRKFRLVVSMQRYAKFKAEEDENA---EFLLRAYPDLQIAYLD--EEPDEEGGEP 236
                          250       260       270       280       290       300       310       320
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063709997 1236 EYYSKLVK--ADI---SGKDKEIYSIKLPGDPKLGEGKPENQNHAIVFTRGNAIQTIDMNQDNYFEEALKMRNLLEEFDR 1310
Cdd:pfam02364  237 EYYSVLIDghCEIdqeNGKRKPKYRIRLSGNPILGDGKSDNQNHAIIFYRGEYIQVIDANQDNYLEECLKIRSVLAEFEE 316
                          330       340       350       360       370       380       390       400
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063709997 1311 DH-GIRPP-------------TILGVREHVFTGSVSSLASFMSNQETSFVTLGQRVLAKpLKIRMHYGHPDVFDRVFHIT 1376
Cdd:pfam02364  317 MNlGIRSPyipgiydeeknpvAILGAREYIFSENIGVLGDIAAGKEQTFGTLFARTLAE-IGGKLHYGHPDFLNAIFMTT 395
                          410       420       430       440       450       460       470       480
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063709997 1377 RGGISKASRVINISEDIFAGFNTTLRQGNVTHHEYIQVGKGRDVGLNQIALFEGKVAGGNGEQVLSRDVYRLGQLLDFFR 1456
Cdd:pfam02364  396 RGGVSKAQKGLHLNEDIYAGMNATLRGGRIKHCEYYQCGKGRDLGFGSILNFTTKIGAGMGEQMLSREYYYLGTQLPLDR 475
                          490       500       510       520       530       540       550       560
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063709997 1457 MMSFFFTTVGFYLCTMLTVLTVYIFLYgrAYLALSGV-----------GATIRERAILLDDTALSAALNAQFLFQIGVFT 1525
Cdd:pfam02364  476 FLSFYYAHPGFHLNNMFIMLSVQLFML--LLLNLGALnhesiiceydkDNPITDPERPIGCYNLQPVLNWVSRFVLSIFI 553
                          570       580       590       600       610       620       630       640
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063709997 1526 A-----VPMVLGFILEQGFLQAIVSFITMQFQLCTVFFTFSLGTRTHYFGRTILHGGARYQATGRGFVVKHIKFSENYRL 1600
Cdd:pfam02364  554 VffisfLPLIVQELLERGFLKAVSRFFKHFLSLSPLFEVFVCQIYAHSLLRNLTFGGARYIATGRGFATTRIPFAELYSR 633
                          650       660       670       680       690       700       710       720
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063709997 1601 YSRSHFVKAMEVILLLVVylaygndeAGAVSYILLTVSSWFLAVSWLFAPYLFNPAGFEWQKVVEDFKEWTNWLfYRGGI 1680
Cdd:pfam02364  634 FARSSIYKGIELFLMLLF--------ATTTMWIPALLWFWITVVSLCLAPFLFNPHQFSWLDFFIDYRDFIRWL-SRGNS 704
                          730       740       750       760       770       780       790       800
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063709997 1681 -------------------GVKGAESWEAWWEEELSHIRTLSGRI--METILSL----------RFFIFQYGI------- 1722
Cdd:pfam02364  705 kthenswigyerqsrlritGYKRKLLGDPSEKLSGDVPRASFTNLffSEIILPLivallifiayRFINSQYGVrgpkptn 784
                          810       820       830
                   ....*....|....*....|....*....|....*.
gi 1063709997 1723 -VYKLKLqgsdTSFAVYGWSWVafaMIIVLFKVFTF 1757
Cdd:pfam02364  785 sVYRLAI----VSILPILLNWI---VLLVLFGISCL 813
FKS1_dom1 pfam14288
1,3-beta-glucan synthase subunit FKS1, domain-1; The FKS1_dom1 domain is likely to be the ...
333-448 2.98e-54

1,3-beta-glucan synthase subunit FKS1, domain-1; The FKS1_dom1 domain is likely to be the 'Class I' region just N-terminal to the first set of transmembrane helices that is involved in 1,3-beta-glucan synthesis itself. This family is found on proteins with family Glucan_synthase, pfam02364.


Pssm-ID: 464126  Cd Length: 112  Bit Score: 184.73  E-value: 2.98e-54
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063709997  333 KKLLFLSLYFLIWGEAANIRFLPECLCYIFHHMVREMDEILRQQVARPAESCmpvdsrGSDDGVSFLDHVIAPLYGVV-S 411
Cdd:pfam14288    1 RKLLQIALYLLIWGEAANVRFMPECLCYIFHCMAYELNGILDGNVSPMTYSP------YSGPEGSFLDNVITPIYRFIrD 74
                           90       100       110
                   ....*....|....*....|....*....|....*...
gi 1063709997  412 AEAFNNDNGRAPHSAWRNYDDFNEYFWSLHSFE-LGWP 448
Cdd:pfam14288   75 QEYEISKNGEADHSAWIGYDDINQLFWSPECIErLGWP 112
 
Name Accession Description Interval E-value
Glucan_synthase pfam02364
1,3-beta-glucan synthase component; This family consists of various 1,3-beta-glucan synthase ...
1025-1757 0e+00

1,3-beta-glucan synthase component; This family consists of various 1,3-beta-glucan synthase components including Gls1, Gls2 and Gls3 from yeast. 1,3-beta-glucan synthase EC:2.4.1.34 also known as callose synthase catalyzes the formation of a beta-1,3-glucan polymer that is a major component of the fungal cell wall. The reaction catalyzed is:- UDP-glucose + {(1,3)-beta-D-glucosyl}(N) <=> UDP + {(1,3)-beta-D-glucosyl}(N+1).


Pssm-ID: 426739  Cd Length: 818  Bit Score: 840.30  E-value: 0e+00
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063709997 1025 VPRNLEARRRLQFFTNSLFMDVPPPKSVRKMLSFSVFTPYYSEVVLYSMAELTKRNEDG--ISILFYLQKIYPDEWKNFL 1102
Cdd:pfam02364    2 FPKNSEAERRISFFAQSLSTPMPEPPPVEKMPTFTVLIPHYSEKILLSLREIIREEEDGsrVTLLEYLKQLHPDEWKNFV 81
                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063709997 1103 ARIGR----DENALEGDLDNERD-----------------------ILELRFWASYRGQTLARTVRGMMYYRKALMLQSY 1155
Cdd:pfam02364   82 EDTKLlaeeDDADDSNSEKDEEDlvkekiddlpfycigfksstpeyTLRTRIWASLRGQTLYRTVSGFMNYSRAIKLLYR 161
                          170       180       190       200       210       220       230       240
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063709997 1156 LERKAGNDATDAEGFELSPEARAQADLKFTYVVTCQIYGRQKEDQKPEAvdiALLMQRNEALRIAYIDvvDSPKEGKSHT 1235
Cdd:pfam02364  162 VENPSLVQLYSGNSEKLERELESMALRKFRLVVSMQRYAKFKAEEDENA---EFLLRAYPDLQIAYLD--EEPDEEGGEP 236
                          250       260       270       280       290       300       310       320
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063709997 1236 EYYSKLVK--ADI---SGKDKEIYSIKLPGDPKLGEGKPENQNHAIVFTRGNAIQTIDMNQDNYFEEALKMRNLLEEFDR 1310
Cdd:pfam02364  237 EYYSVLIDghCEIdqeNGKRKPKYRIRLSGNPILGDGKSDNQNHAIIFYRGEYIQVIDANQDNYLEECLKIRSVLAEFEE 316
                          330       340       350       360       370       380       390       400
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063709997 1311 DH-GIRPP-------------TILGVREHVFTGSVSSLASFMSNQETSFVTLGQRVLAKpLKIRMHYGHPDVFDRVFHIT 1376
Cdd:pfam02364  317 MNlGIRSPyipgiydeeknpvAILGAREYIFSENIGVLGDIAAGKEQTFGTLFARTLAE-IGGKLHYGHPDFLNAIFMTT 395
                          410       420       430       440       450       460       470       480
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063709997 1377 RGGISKASRVINISEDIFAGFNTTLRQGNVTHHEYIQVGKGRDVGLNQIALFEGKVAGGNGEQVLSRDVYRLGQLLDFFR 1456
Cdd:pfam02364  396 RGGVSKAQKGLHLNEDIYAGMNATLRGGRIKHCEYYQCGKGRDLGFGSILNFTTKIGAGMGEQMLSREYYYLGTQLPLDR 475
                          490       500       510       520       530       540       550       560
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063709997 1457 MMSFFFTTVGFYLCTMLTVLTVYIFLYgrAYLALSGV-----------GATIRERAILLDDTALSAALNAQFLFQIGVFT 1525
Cdd:pfam02364  476 FLSFYYAHPGFHLNNMFIMLSVQLFML--LLLNLGALnhesiiceydkDNPITDPERPIGCYNLQPVLNWVSRFVLSIFI 553
                          570       580       590       600       610       620       630       640
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063709997 1526 A-----VPMVLGFILEQGFLQAIVSFITMQFQLCTVFFTFSLGTRTHYFGRTILHGGARYQATGRGFVVKHIKFSENYRL 1600
Cdd:pfam02364  554 VffisfLPLIVQELLERGFLKAVSRFFKHFLSLSPLFEVFVCQIYAHSLLRNLTFGGARYIATGRGFATTRIPFAELYSR 633
                          650       660       670       680       690       700       710       720
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063709997 1601 YSRSHFVKAMEVILLLVVylaygndeAGAVSYILLTVSSWFLAVSWLFAPYLFNPAGFEWQKVVEDFKEWTNWLfYRGGI 1680
Cdd:pfam02364  634 FARSSIYKGIELFLMLLF--------ATTTMWIPALLWFWITVVSLCLAPFLFNPHQFSWLDFFIDYRDFIRWL-SRGNS 704
                          730       740       750       760       770       780       790       800
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063709997 1681 -------------------GVKGAESWEAWWEEELSHIRTLSGRI--METILSL----------RFFIFQYGI------- 1722
Cdd:pfam02364  705 kthenswigyerqsrlritGYKRKLLGDPSEKLSGDVPRASFTNLffSEIILPLivallifiayRFINSQYGVrgpkptn 784
                          810       820       830
                   ....*....|....*....|....*....|....*.
gi 1063709997 1723 -VYKLKLqgsdTSFAVYGWSWVafaMIIVLFKVFTF 1757
Cdd:pfam02364  785 sVYRLAI----VSILPILLNWI---VLLVLFGISCL 813
FKS1_dom1 pfam14288
1,3-beta-glucan synthase subunit FKS1, domain-1; The FKS1_dom1 domain is likely to be the ...
333-448 2.98e-54

1,3-beta-glucan synthase subunit FKS1, domain-1; The FKS1_dom1 domain is likely to be the 'Class I' region just N-terminal to the first set of transmembrane helices that is involved in 1,3-beta-glucan synthesis itself. This family is found on proteins with family Glucan_synthase, pfam02364.


Pssm-ID: 464126  Cd Length: 112  Bit Score: 184.73  E-value: 2.98e-54
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063709997  333 KKLLFLSLYFLIWGEAANIRFLPECLCYIFHHMVREMDEILRQQVARPAESCmpvdsrGSDDGVSFLDHVIAPLYGVV-S 411
Cdd:pfam14288    1 RKLLQIALYLLIWGEAANVRFMPECLCYIFHCMAYELNGILDGNVSPMTYSP------YSGPEGSFLDNVITPIYRFIrD 74
                           90       100       110
                   ....*....|....*....|....*....|....*...
gi 1063709997  412 AEAFNNDNGRAPHSAWRNYDDFNEYFWSLHSFE-LGWP 448
Cdd:pfam14288   75 QEYEISKNGEADHSAWIGYDDINQLFWSPECIErLGWP 112
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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