NCBI Home Page NCBI Site Search page NCBI Guide that lists and describes the NCBI resources
Conserved domains on  [gi|1063695744|ref|NP_001321009|]
View 

Rhomboid-related intramembrane serine protease family protein [Arabidopsis thaliana]

Protein Classification

rhomboid family protein( domain architecture ID 10483871)

rhomboid family protein may be an intramembrane serine protease that catalyzes regulated intramembrane proteolysis, resulting in the release of functional polypeptides from their membrane anchors, or an inactive rhomboid protein that lacks the conserved active sites and has no peptidase activity

CATH:  1.20.1540.10
Gene Ontology:  GO:0016020
MEROPS:  S54
PubMed:  24099005|22035660|26527717|12620104
SCOP:  4000471

Graphical summary

 Zoom to residue level

show extra options »

Show site features     Horizontal zoom: ×

List of domain hits

 Name Accession Description Interval E-value
Rhomboid pfam01694
Rhomboid family; This family contains integral membrane proteins that are related to ...
 119-256 2.56e-31

Rhomboid family; This family contains integral membrane proteins that are related to Drosophila rhomboid protein. Members of this family are found in bacteria and eukaryotes. Rhomboid promotes the cleavage of the membrane-anchored TGF-alpha-like growth factor Spitz, allowing it to activate the Drosophila EGF receptor. Analysis has shown that Rhomboid-1 is an intramembrane serine protease (EC:3.4.21.105). Parasite-encoded rhomboid enzymes are also important for invasion of host cells by Toxoplasma and the malaria parasite.


:

Pssm-ID: 426384  Cd Length: 147  Bit Score: 116.17  E-value: 2.56e-31
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063695744 119 NHEIWRILTSPWLHSGLFHLFINLGSLIFVGIYMEQQFGPLRIAVIYFLSGIMGSLFAVLFVR-NIPSISSGAAFFGLIG 197
Cdd:pfam01694   4 PGQLWRLITSMFLHAGWLHLLFNMLALLFFGGPLERILGSVRFLLLYLLSGIAGSLLSYLFSPlSTPSVGASGAIFGLLG 83

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|.
gi 1063695744 198 AMLSALAKNWNLYNSKISALAIIFTIFTVNFLIGFLP--FIDNFANIGGFISGFLLGFVLL 256
Cdd:pfam01694  84 ALLVLGPRNRILLFGLIGALLALLLFILLNLVLGLLPgnGVSNLAHLGGLLVGLLLGFILL 144
 
Name Accession Description Interval E-value
Rhomboid pfam01694
Rhomboid family; This family contains integral membrane proteins that are related to ...
 119-256 2.56e-31

Rhomboid family; This family contains integral membrane proteins that are related to Drosophila rhomboid protein. Members of this family are found in bacteria and eukaryotes. Rhomboid promotes the cleavage of the membrane-anchored TGF-alpha-like growth factor Spitz, allowing it to activate the Drosophila EGF receptor. Analysis has shown that Rhomboid-1 is an intramembrane serine protease (EC:3.4.21.105). Parasite-encoded rhomboid enzymes are also important for invasion of host cells by Toxoplasma and the malaria parasite.


Pssm-ID: 426384  Cd Length: 147  Bit Score: 116.17  E-value: 2.56e-31
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063695744 119 NHEIWRILTSPWLHSGLFHLFINLGSLIFVGIYMEQQFGPLRIAVIYFLSGIMGSLFAVLFVR-NIPSISSGAAFFGLIG 197
Cdd:pfam01694   4 PGQLWRLITSMFLHAGWLHLLFNMLALLFFGGPLERILGSVRFLLLYLLSGIAGSLLSYLFSPlSTPSVGASGAIFGLLG 83

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|.
gi 1063695744 198 AMLSALAKNWNLYNSKISALAIIFTIFTVNFLIGFLP--FIDNFANIGGFISGFLLGFVLL 256
Cdd:pfam01694  84 ALLVLGPRNRILLFGLIGALLALLLFILLNLVLGLLPgnGVSNLAHLGGLLVGLLLGFILL 144
GlpG COG0705
Membrane-associated serine protease, rhomboid family [Posttranslational modification, protein ...
 115-256 5.93e-28

Membrane-associated serine protease, rhomboid family [Posttranslational modification, protein turnover, chaperones];


Pssm-ID: 440469 [Multi-domain]  Cd Length: 189  Bit Score: 108.41  E-value: 5.93e-28
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063695744 115 ALTENHEIWRILTSPWLHSGLFHLFINLGSLIFVGIYMEQQFGPLRIAVIYFLSGIMGSLFAVLFVRNIPSISSGA--AF 192
Cdd:COG0705    36 ARLLLGELWRLLTSMFLHGGFLHLLFNMLALWVFGPLLERRLGSKRFLLLYLLSGLGGGLLQLLFSPGSGYPLVGAsgAI 115

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 1063695744 193 FGLIGAMLsALAKN--WNLYNSKISALAIIFTIFTVNFLIGFL--PFIDNFANIGGFISGFLLGFVLL 256
Cdd:COG0705   116 FGLLGALL-VLGPRrrVLLLFIPIPALLFLLVWLLLGLLFGLLggGGIAWEAHLGGLLAGLLLALLLR 182
PTZ00101 PTZ00101
rhomboid-1 protease; Provisional
 98-265 1.32e-14

rhomboid-1 protease; Provisional


Pssm-ID: 185445  Cd Length: 278  Bit Score: 73.35  E-value: 1.32e-14
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063695744  98 LGPSASTLEHMGGlSWKALTENHEIWRILTSPWLHSGLFHLFINLGSLIFVGIYMEQQFGPLRIAVIYFLSGIMGSLFAV 177
Cdd:PTZ00101   79 LTPSDSLLVTLGA-NVASRIKQGEIHRLILPIFLHANIFHTFFNVFFQLRMGFTLEKNYGIVKIIILYFLTGIYGNILSS 157

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063695744 178 LFVRNIPSISSGAAFFGLIGAMLSALAKNWN--------LYNSKISALAIIFTIFTVNFligflPFIDNFANIGGFISGF 249
Cdd:PTZ00101  158 SVTYCPIKVGASTSGMGLLGIVTSELILLWHvirhrervVFNIIFFSLISFFYYFTFNG-----SNIDHVGHLGGLLSGI 232

                         170
                  ....*....|....*.
gi 1063695744 250 LLGfvLLFKPQLRQMP 265
Cdd:PTZ00101  233 SMG--ILYNSQMENKP 246
rhom_GG_sort TIGR03902
rhomboid family GlyGly-CTERM serine protease; This model describes a rhomboid-like ...
 113-253 1.04e-07

rhomboid family GlyGly-CTERM serine protease; This model describes a rhomboid-like intramembrane serine protease. Its species distribution closely matches model TIGR03501, GlyGly-CTERM, which describes a protein targeting domain analogous to LPXTG and PEP-CTERM. In a number of species (Ralstonia eutropha ,R. metallidurans, R. solanacearum, Marinobacter aquaeolei, etc) with just one GlyGly-CTERM protein (i.e., a dedicated system), the rhombosortase and GlyGly-CTERM genes are adjacent.


Pssm-ID: 274845  Cd Length: 154  Bit Score: 51.03  E-value: 1.04e-07
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063695744 113 WKALTENHEIWRILTSPWLHSGLFHLFINLGSLIFVGIYMEQQFGPLRIAVIYFLSGIMGSLFAVLFVrniPSISSGAAF 192
Cdd:TIGR03902   7 DRAAILDGEWWRLLTGHFVHLNWWHLLMNLAGLLLLWALFGRHLRARRLLLLLLLLSLLISLGLLLFL---PSLQWYVGL 83

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 1063695744 193 FGLIGAMLSALAKNWNLYNSKISALAIIFTIFTVNF------------LIGFLPFIDnfANIGGFISGFLLGF 253
Cdd:TIGR03902  84 SGVLHGLFAWGALRDIRYGRRSGWLLLLGVIAKLAWeqlfgasaftaaLIGAPVATE--AHLAGAISGLLIAL 154
MFS_NepI_like cd17324
Purine ribonucleoside efflux pump NepI and similar transporters of the Major Facilitator ...
 166-309 2.18e-03

Purine ribonucleoside efflux pump NepI and similar transporters of the Major Facilitator Superfamily; This family is composed of purine efflux pumps such as Escherichia coli NepI and Bacillus subtilis PbuE, sugar efflux transporters such as Corynebacterium glutamicum arabinose efflux permease, multidrug resistance (MDR) transporters such as Streptomyces lividans chloramphenicol resistance protein (CmlR), and similar proteins. NepI and PbuE are involved in the efflux of purine ribonucleosides such as guanosine, adenosine and inosine, as well as purine bases like guanine, adenine, and hypoxanthine, and purine base analogs. They play a role in the maintenance of cellular purine base pools, as well as in protecting the cells and conferring resistance against toxic purine base analogs such as 6-mercaptopurine. MDR transporters are drug/H+ antiporters (DHA) that mediate the efflux of a variety of drugs and toxic compounds, and confer resistance to these compounds. The NepI-like family belongs to the Major Facilitator Superfamily (MFS) of membrane transport proteins, which are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.


Pssm-ID: 340882 [Multi-domain]  Cd Length: 370  Bit Score: 39.84  E-value: 2.18e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063695744 166 FLSGIMGSLFAVLFVRNIPSISSGAaFFGLIGAMLSALAKnwnlYNSKISALAIIFTIFTVNFLIGfLPFIDNFANIGG- 244
Cdd:cd17324    79 LLAALAPSFALLLLARALAGLAHGG-FWAIAAAYAADLVP----PEKRGRAIGLVFSGLTLGLVLG-RPLGGLLGQLLGw 152

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063695744 245 -----FISGFLLGFVLLFKPQLRQMPPSHKGKLfeddmNRSTRLKEQFDRPVLRIICLLVFCGILAGVLL 309
Cdd:cd17324   153 raaflAIAVLALLAALLLWRLLPSLPPKKPGSL-----GLLSSLLLLLRNPRLRLAYLITFLLFGGFFAL 217
 
Name Accession Description Interval E-value
Rhomboid pfam01694
Rhomboid family; This family contains integral membrane proteins that are related to ...
 119-256 2.56e-31

Rhomboid family; This family contains integral membrane proteins that are related to Drosophila rhomboid protein. Members of this family are found in bacteria and eukaryotes. Rhomboid promotes the cleavage of the membrane-anchored TGF-alpha-like growth factor Spitz, allowing it to activate the Drosophila EGF receptor. Analysis has shown that Rhomboid-1 is an intramembrane serine protease (EC:3.4.21.105). Parasite-encoded rhomboid enzymes are also important for invasion of host cells by Toxoplasma and the malaria parasite.


Pssm-ID: 426384  Cd Length: 147  Bit Score: 116.17  E-value: 2.56e-31
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063695744 119 NHEIWRILTSPWLHSGLFHLFINLGSLIFVGIYMEQQFGPLRIAVIYFLSGIMGSLFAVLFVR-NIPSISSGAAFFGLIG 197
Cdd:pfam01694   4 PGQLWRLITSMFLHAGWLHLLFNMLALLFFGGPLERILGSVRFLLLYLLSGIAGSLLSYLFSPlSTPSVGASGAIFGLLG 83

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|.
gi 1063695744 198 AMLSALAKNWNLYNSKISALAIIFTIFTVNFLIGFLP--FIDNFANIGGFISGFLLGFVLL 256
Cdd:pfam01694  84 ALLVLGPRNRILLFGLIGALLALLLFILLNLVLGLLPgnGVSNLAHLGGLLVGLLLGFILL 144
GlpG COG0705
Membrane-associated serine protease, rhomboid family [Posttranslational modification, protein ...
 115-256 5.93e-28

Membrane-associated serine protease, rhomboid family [Posttranslational modification, protein turnover, chaperones];


Pssm-ID: 440469 [Multi-domain]  Cd Length: 189  Bit Score: 108.41  E-value: 5.93e-28
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063695744 115 ALTENHEIWRILTSPWLHSGLFHLFINLGSLIFVGIYMEQQFGPLRIAVIYFLSGIMGSLFAVLFVRNIPSISSGA--AF 192
Cdd:COG0705    36 ARLLLGELWRLLTSMFLHGGFLHLLFNMLALWVFGPLLERRLGSKRFLLLYLLSGLGGGLLQLLFSPGSGYPLVGAsgAI 115

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 1063695744 193 FGLIGAMLsALAKN--WNLYNSKISALAIIFTIFTVNFLIGFL--PFIDNFANIGGFISGFLLGFVLL 256
Cdd:COG0705   116 FGLLGALL-VLGPRrrVLLLFIPIPALLFLLVWLLLGLLFGLLggGGIAWEAHLGGLLAGLLLALLLR 182
PTZ00101 PTZ00101
rhomboid-1 protease; Provisional
 98-265 1.32e-14

rhomboid-1 protease; Provisional


Pssm-ID: 185445  Cd Length: 278  Bit Score: 73.35  E-value: 1.32e-14
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063695744  98 LGPSASTLEHMGGlSWKALTENHEIWRILTSPWLHSGLFHLFINLGSLIFVGIYMEQQFGPLRIAVIYFLSGIMGSLFAV 177
Cdd:PTZ00101   79 LTPSDSLLVTLGA-NVASRIKQGEIHRLILPIFLHANIFHTFFNVFFQLRMGFTLEKNYGIVKIIILYFLTGIYGNILSS 157

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063695744 178 LFVRNIPSISSGAAFFGLIGAMLSALAKNWN--------LYNSKISALAIIFTIFTVNFligflPFIDNFANIGGFISGF 249
Cdd:PTZ00101  158 SVTYCPIKVGASTSGMGLLGIVTSELILLWHvirhrervVFNIIFFSLISFFYYFTFNG-----SNIDHVGHLGGLLSGI 232

                         170
                  ....*....|....*.
gi 1063695744 250 LLGfvLLFKPQLRQMP 265
Cdd:PTZ00101  233 SMG--ILYNSQMENKP 246
rhom_GG_sort TIGR03902
rhomboid family GlyGly-CTERM serine protease; This model describes a rhomboid-like ...
 113-253 1.04e-07

rhomboid family GlyGly-CTERM serine protease; This model describes a rhomboid-like intramembrane serine protease. Its species distribution closely matches model TIGR03501, GlyGly-CTERM, which describes a protein targeting domain analogous to LPXTG and PEP-CTERM. In a number of species (Ralstonia eutropha ,R. metallidurans, R. solanacearum, Marinobacter aquaeolei, etc) with just one GlyGly-CTERM protein (i.e., a dedicated system), the rhombosortase and GlyGly-CTERM genes are adjacent.


Pssm-ID: 274845  Cd Length: 154  Bit Score: 51.03  E-value: 1.04e-07
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063695744 113 WKALTENHEIWRILTSPWLHSGLFHLFINLGSLIFVGIYMEQQFGPLRIAVIYFLSGIMGSLFAVLFVrniPSISSGAAF 192
Cdd:TIGR03902   7 DRAAILDGEWWRLLTGHFVHLNWWHLLMNLAGLLLLWALFGRHLRARRLLLLLLLLSLLISLGLLLFL---PSLQWYVGL 83

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 1063695744 193 FGLIGAMLSALAKNWNLYNSKISALAIIFTIFTVNF------------LIGFLPFIDnfANIGGFISGFLLGF 253
Cdd:TIGR03902  84 SGVLHGLFAWGALRDIRYGRRSGWLLLLGVIAKLAWeqlfgasaftaaLIGAPVATE--AHLAGAISGLLIAL 154
MFS_NepI_like cd17324
Purine ribonucleoside efflux pump NepI and similar transporters of the Major Facilitator ...
 166-309 2.18e-03

Purine ribonucleoside efflux pump NepI and similar transporters of the Major Facilitator Superfamily; This family is composed of purine efflux pumps such as Escherichia coli NepI and Bacillus subtilis PbuE, sugar efflux transporters such as Corynebacterium glutamicum arabinose efflux permease, multidrug resistance (MDR) transporters such as Streptomyces lividans chloramphenicol resistance protein (CmlR), and similar proteins. NepI and PbuE are involved in the efflux of purine ribonucleosides such as guanosine, adenosine and inosine, as well as purine bases like guanine, adenine, and hypoxanthine, and purine base analogs. They play a role in the maintenance of cellular purine base pools, as well as in protecting the cells and conferring resistance against toxic purine base analogs such as 6-mercaptopurine. MDR transporters are drug/H+ antiporters (DHA) that mediate the efflux of a variety of drugs and toxic compounds, and confer resistance to these compounds. The NepI-like family belongs to the Major Facilitator Superfamily (MFS) of membrane transport proteins, which are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.


Pssm-ID: 340882 [Multi-domain]  Cd Length: 370  Bit Score: 39.84  E-value: 2.18e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063695744 166 FLSGIMGSLFAVLFVRNIPSISSGAaFFGLIGAMLSALAKnwnlYNSKISALAIIFTIFTVNFLIGfLPFIDNFANIGG- 244
Cdd:cd17324    79 LLAALAPSFALLLLARALAGLAHGG-FWAIAAAYAADLVP----PEKRGRAIGLVFSGLTLGLVLG-RPLGGLLGQLLGw 152

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1063695744 245 -----FISGFLLGFVLLFKPQLRQMPPSHKGKLfeddmNRSTRLKEQFDRPVLRIICLLVFCGILAGVLL 309
Cdd:cd17324   153 raaflAIAVLALLAALLLWRLLPSLPPKKPGSL-----GLLSSLLLLLRNPRLRLAYLITFLLFGGFFAL 217
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
Help | Disclaimer | Write to the Help Desk
NCBI | NLM | NIH