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Conserved domains on  [gi|442634435|ref|NP_001263160|]
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sarcoplasmic calcium-binding protein 1, isoform B [Drosophila melanogaster]

Protein Classification

EF-hand domain-containing protein( domain architecture ID 1000101)

EF-hand (EFh) domain-containing protein may be involved in binding intracellular calcium and in calcium signal transduction

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
FRQ1 super family cl34916
Ca2+-binding protein, EF-hand superfamily [Signal transduction mechanisms];
16-127 2.38e-08

Ca2+-binding protein, EF-hand superfamily [Signal transduction mechanisms];


The actual alignment was detected with superfamily member COG5126:

Pssm-ID: 444056 [Multi-domain]  Cd Length: 137  Bit Score: 50.56  E-value: 2.38e-08
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 442634435  16 MYDIDNNGFLDQndflcmavracvvegkgdcstarlDDYKKLMKNLWDEISAIADDDKDGKISNQEFKDAVKKtcvgkky 95
Cdd:COG5126   13 LLDADGDGVLER------------------------DDFEALFRRLWATLFSEADTDGDGRISREEFVAGMES------- 61

                         90       100       110
                 ....*....|....*....|....*....|..
gi 442634435  96 eEFPQAMRAFIESNFKLLDIDSDGIVGVKEYR 127
Cdd:COG5126   62 -LFEATVEPFARAAFDLLDTDGDGKISADEFR 92
 
Name Accession Description Interval E-value
FRQ1 COG5126
Ca2+-binding protein, EF-hand superfamily [Signal transduction mechanisms];
16-127 2.38e-08

Ca2+-binding protein, EF-hand superfamily [Signal transduction mechanisms];


Pssm-ID: 444056 [Multi-domain]  Cd Length: 137  Bit Score: 50.56  E-value: 2.38e-08
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 442634435  16 MYDIDNNGFLDQndflcmavracvvegkgdcstarlDDYKKLMKNLWDEISAIADDDKDGKISNQEFKDAVKKtcvgkky 95
Cdd:COG5126   13 LLDADGDGVLER------------------------DDFEALFRRLWATLFSEADTDGDGRISREEFVAGMES------- 61

                         90       100       110
                 ....*....|....*....|....*....|..
gi 442634435  96 eEFPQAMRAFIESNFKLLDIDSDGIVGVKEYR 127
Cdd:COG5126   62 -LFEATVEPFARAAFDLLDTDGDGKISADEFR 92
EFh_MICU3 cd16175
EF-hand, calcium binding motif, found in calcium uptake protein 3, mitochondrial (MICU3) and ...
 16-126 1.30e-04

EF-hand, calcium binding motif, found in calcium uptake protein 3, mitochondrial (MICU3) and similar proteins; MICU3, also termed EF-hand domain-containing family member A2 (EFHA2), is a paralog of MICU1 and notably found in the central nervous system (CNS) and skeletal muscle. At present, the precise molecular function of MICU3 remains unclear. It likely has a role in mitochondrial calcium handling. MICU3 contains an N-terminal mitochondrial targeting sequence (MTS) as well as two evolutionarily conserved canonical Ca2+-binding EF-hands separated by a long stretch of residues predicted to form alpha-helices.


Pssm-ID: 320083 [Multi-domain]  Cd Length: 128  Bit Score: 40.19  E-value: 1.30e-04
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 442634435  16 MYDIDNNGFLDQNDFLCMA--VRACVVEGKGDCSTARL--DDYKKLMKNLWDEISAIADDDK-----DGKISNQEFKDAV 86
Cdd:cd16175    8 MFDTDGNEMVDKKEFLVLQeiFRTLLVHFFGKKGKAELnfEDFYRFMDNLQTEVEDFTIAMRmytfaDRSISQDEFARAV 87

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|
gi 442634435  87 KkTCVGKKYEefpqamRAFIESNFKLLDIDSDGIVGVKEY 126
Cdd:cd16175   88 K-VCTGLKLS------PHLVNTVFKIFDVDGDGQLSYKEF 120
 
Name Accession Description Interval E-value
FRQ1 COG5126
Ca2+-binding protein, EF-hand superfamily [Signal transduction mechanisms];
16-127 2.38e-08

Ca2+-binding protein, EF-hand superfamily [Signal transduction mechanisms];


Pssm-ID: 444056 [Multi-domain]  Cd Length: 137  Bit Score: 50.56  E-value: 2.38e-08
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 442634435  16 MYDIDNNGFLDQndflcmavracvvegkgdcstarlDDYKKLMKNLWDEISAIADDDKDGKISNQEFKDAVKKtcvgkky 95
Cdd:COG5126   13 LLDADGDGVLER------------------------DDFEALFRRLWATLFSEADTDGDGRISREEFVAGMES------- 61

                         90       100       110
                 ....*....|....*....|....*....|..
gi 442634435  96 eEFPQAMRAFIESNFKLLDIDSDGIVGVKEYR 127
Cdd:COG5126   62 -LFEATVEPFARAAFDLLDTDGDGKISADEFR 92
EFh_MICU3 cd16175
EF-hand, calcium binding motif, found in calcium uptake protein 3, mitochondrial (MICU3) and ...
 16-126 1.30e-04

EF-hand, calcium binding motif, found in calcium uptake protein 3, mitochondrial (MICU3) and similar proteins; MICU3, also termed EF-hand domain-containing family member A2 (EFHA2), is a paralog of MICU1 and notably found in the central nervous system (CNS) and skeletal muscle. At present, the precise molecular function of MICU3 remains unclear. It likely has a role in mitochondrial calcium handling. MICU3 contains an N-terminal mitochondrial targeting sequence (MTS) as well as two evolutionarily conserved canonical Ca2+-binding EF-hands separated by a long stretch of residues predicted to form alpha-helices.


Pssm-ID: 320083 [Multi-domain]  Cd Length: 128  Bit Score: 40.19  E-value: 1.30e-04
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 442634435  16 MYDIDNNGFLDQNDFLCMA--VRACVVEGKGDCSTARL--DDYKKLMKNLWDEISAIADDDK-----DGKISNQEFKDAV 86
Cdd:cd16175    8 MFDTDGNEMVDKKEFLVLQeiFRTLLVHFFGKKGKAELnfEDFYRFMDNLQTEVEDFTIAMRmytfaDRSISQDEFARAV 87

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|
gi 442634435  87 KkTCVGKKYEefpqamRAFIESNFKLLDIDSDGIVGVKEY 126
Cdd:cd16175   88 K-VCTGLKLS------PHLVNTVFKIFDVDGDGQLSYKEF 120
EFh cd00051
EF-hand, calcium binding motif; A diverse superfamily of calcium sensors and calcium signal ...
 16-87 9.89e-04

EF-hand, calcium binding motif; A diverse superfamily of calcium sensors and calcium signal modulators; most examples in this alignment model have 2 active canonical EF hands. Ca2+ binding induces a conformational change in the EF-hand motif, leading to the activation or inactivation of target proteins. EF-hands tend to occur in pairs or higher copy numbers.


Pssm-ID: 238008 [Multi-domain]  Cd Length: 63  Bit Score: 35.99  E-value: 9.89e-04
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 442634435  16 MYDIDNNGFLDQNDFLcMAVRACvvegkgdcstarlddYKKLMKNLWDEISAIADDDKDGKISNQEFKDAVK 87
Cdd:cd00051    8 LFDKDGDGTISADELK-AALKSL---------------GEGLSEEEIDEMIREVDKDGDGKIDFEEFLELMA 63
EFh cd00051
EF-hand, calcium binding motif; A diverse superfamily of calcium sensors and calcium signal ...
 70-125 1.20e-03

EF-hand, calcium binding motif; A diverse superfamily of calcium sensors and calcium signal modulators; most examples in this alignment model have 2 active canonical EF hands. Ca2+ binding induces a conformational change in the EF-hand motif, leading to the activation or inactivation of target proteins. EF-hands tend to occur in pairs or higher copy numbers.


Pssm-ID: 238008 [Multi-domain]  Cd Length: 63  Bit Score: 35.99  E-value: 1.20e-03
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*.
gi 442634435  70 DDDKDGKISNQEFKDAVKKTCVGKKYEEFPQAMRAFiesnfkllDIDSDGIVGVKE 125
Cdd:cd00051   10 DKDGDGTISADELKAALKSLGEGLSEEEIDEMIREV--------DKDGDGKIDFEE 57
EFh_CREC cd15899
EF-hand, calcium binding motif, found in CREC-EF hand family; The CREC (Cab45/reticulocalbin ...
 70-164 5.01e-03

EF-hand, calcium binding motif, found in CREC-EF hand family; The CREC (Cab45/reticulocalbin/ERC45/calumenin)-EF hand family contains a group of six EF-hand, low-affinity Ca2+-binding proteins, including reticulocalbin (RCN-1), ER Ca2+-binding protein of 55 kDa (ERC-55, also known as TCBP-49 or E6BP), reticulocalbin-3 (RCN-3), Ca2+-binding protein of 45 kDa (Cab45 and its splice variant Cab45b), and calumenin ( also known as crocalbin or CBP-50). The proteins are not only localized in various parts of the secretory pathway, but also found in the cytosolic compartment and at the cell surface. They interact with different ligands or proteins and have been implicated in the secretory process, chaperone activity, signal transduction as well as in a large variety of disease processes.


Pssm-ID: 320021 [Multi-domain]  Cd Length: 267  Bit Score: 36.65  E-value: 5.01e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 442634435  70 DDDKDGKISNQEFKDAVKKTCVGKKYEEfpqamrafIESNFKLLDIDSDGIVGVKEYRYNCITRVA------IDDITPID 143
Cdd:cd15899   45 DVDKDGFISAKELHSWILESFKRHAMEE--------SKEQFRAVDPDEDGHVSWDEYKNDTYGSVGddeenvADNIKEDE 116

                         90       100
                 ....*....|....*....|....*.
gi 442634435 144 KAFETLLNDEDR-----KRGGLSLDR 164
Cdd:cd15899  117 EYKKLLLKDKKRfeaadQDGDLILTL 142
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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