Sterile alpha motif (SAM)/Pointed domain of GA-binding protein (GABP) alpha chain; SAM Pointed domain of GA-binding protein (GABP) alpha subfamily of ETS transcriptional regulators is a putative protein-protein interaction domain. This type of transcriptional regulators forms heterotetramers containing two alpha and two beta subunits. It interacts with GA repeats (purine rich repeats). GABP transcriptional factors control gene expression in cell cycle control, apoptosis, and cellular respiration. GABP participates in regulation of transmembrane receptors and key hormones especially in myeloid cells and at the neuromuscular junction.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 442621308 184 VLDSKFKREQIRLKIPEAANEWTHAHVTYWLEWAVKQFELVGINMSDWQMNGQELCAMTHEEFNQKLPRDPGNIFWTHLQ 263
Cdd:cd08534    1 AVDSNYRKEQERLKIPYDPMEWTEDHVLHWLQWAVKEFGLTGINLSDWNITGRELCSLTQEEFLQRVPKDPGDIFWTHLE 80

                 ....*....
gi 442621308 264 LLKECNFVS 272
Cdd:cd08534   81 LLRKCKFVS 89

GA-binding protein alpha chain; This family of proteins represents the transcription factor GABP alpha. This alpha domain is a five-stranded beta-sheet crossed by a distorted helix termed an OST domain. The surface of the GABP alpha OST domain contains two clusters of negatively-charged residues suggesting there are positively-charged partner proteins. The OST domain binds to the CH1 and CH3 domains of the co-activator histone acetyltransferase CBP/p300, a direct link between GABP and transcriptional machinery has been made.

                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 442621308   48 VHMDIREPLSMLKSLVEQKIGVCLNYYTFWLQDAQELESHKNLVDQCVKGEGLVQINVQIQTI---RKRINIADVLKPT 123
Cdd:pfam11620   1 QHIDINEPLSTLRDLLEPRLQIDLSKYEFWLQDNQMLDPDKSLVDQCVKGEGLVQINVQVISElgnEPRINILDILKPA 79

Sterile alpha motif (SAM)/Pointed domain of GA-binding protein (GABP) alpha chain; SAM Pointed domain of GA-binding protein (GABP) alpha subfamily of ETS transcriptional regulators is a putative protein-protein interaction domain. This type of transcriptional regulators forms heterotetramers containing two alpha and two beta subunits. It interacts with GA repeats (purine rich repeats). GABP transcriptional factors control gene expression in cell cycle control, apoptosis, and cellular respiration. GABP participates in regulation of transmembrane receptors and key hormones especially in myeloid cells and at the neuromuscular junction.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 442621308 184 VLDSKFKREQIRLKIPEAANEWTHAHVTYWLEWAVKQFELVGINMSDWQMNGQELCAMTHEEFNQKLPRDPGNIFWTHLQ 263
Cdd:cd08534    1 AVDSNYRKEQERLKIPYDPMEWTEDHVLHWLQWAVKEFGLTGINLSDWNITGRELCSLTQEEFLQRVPKDPGDIFWTHLE 80

                 ....*....
gi 442621308 264 LLKECNFVS 272
Cdd:cd08534   81 LLRKCKFVS 89

Ets-domain;

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 442621308  347 QLWQFLLEILTDCEHTDVIEWV-GTEGEFKLTDPDRVARLWGEKKNKPAMNYEKLSRALRYYYDGDMISKVSGKRFAYKF 425
Cdd:pfam00178   1 QLWQFLLDLLTDPEYSDIIKWTdKEEGEFRLVDPEAVARLWGKRKGNPKMTYEKLSRALRYYYKKGILEKVPGKRLTYRF 80

GA-binding protein alpha chain; This family of proteins represents the transcription factor GABP alpha. This alpha domain is a five-stranded beta-sheet crossed by a distorted helix termed an OST domain. The surface of the GABP alpha OST domain contains two clusters of negatively-charged residues suggesting there are positively-charged partner proteins. The OST domain binds to the CH1 and CH3 domains of the co-activator histone acetyltransferase CBP/p300, a direct link between GABP and transcriptional machinery has been made.

                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 442621308   48 VHMDIREPLSMLKSLVEQKIGVCLNYYTFWLQDAQELESHKNLVDQCVKGEGLVQINVQIQTI---RKRINIADVLKPT 123
Cdd:pfam11620   1 QHIDINEPLSTLRDLLEPRLQIDLSKYEFWLQDNQMLDPDKSLVDQCVKGEGLVQINVQVISElgnEPRINILDILKPA 79

Sterile alpha motif (SAM)/Pointed domain of GA-binding protein (GABP) alpha chain; SAM Pointed domain of GA-binding protein (GABP) alpha subfamily of ETS transcriptional regulators is a putative protein-protein interaction domain. This type of transcriptional regulators forms heterotetramers containing two alpha and two beta subunits. It interacts with GA repeats (purine rich repeats). GABP transcriptional factors control gene expression in cell cycle control, apoptosis, and cellular respiration. GABP participates in regulation of transmembrane receptors and key hormones especially in myeloid cells and at the neuromuscular junction.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 442621308 184 VLDSKFKREQIRLKIPEAANEWTHAHVTYWLEWAVKQFELVGINMSDWQMNGQELCAMTHEEFNQKLPRDPGNIFWTHLQ 263
Cdd:cd08534    1 AVDSNYRKEQERLKIPYDPMEWTEDHVLHWLQWAVKEFGLTGINLSDWNITGRELCSLTQEEFLQRVPKDPGDIFWTHLE 80

                 ....*....
gi 442621308 264 LLKECNFVS 272
Cdd:cd08534   81 LLRKCKFVS 89

Ets-domain;

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 442621308  347 QLWQFLLEILTDCEHTDVIEWV-GTEGEFKLTDPDRVARLWGEKKNKPAMNYEKLSRALRYYYDGDMISKVSGKRFAYKF 425
Cdd:pfam00178   1 QLWQFLLDLLTDPEYSDIIKWTdKEEGEFRLVDPEAVARLWGKRKGNPKMTYEKLSRALRYYYKKGILEKVPGKRLTYRF 80

GA-binding protein alpha chain; This family of proteins represents the transcription factor GABP alpha. This alpha domain is a five-stranded beta-sheet crossed by a distorted helix termed an OST domain. The surface of the GABP alpha OST domain contains two clusters of negatively-charged residues suggesting there are positively-charged partner proteins. The OST domain binds to the CH1 and CH3 domains of the co-activator histone acetyltransferase CBP/p300, a direct link between GABP and transcriptional machinery has been made.

                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 442621308   48 VHMDIREPLSMLKSLVEQKIGVCLNYYTFWLQDAQELESHKNLVDQCVKGEGLVQINVQIQTI---RKRINIADVLKPT 123
Cdd:pfam11620   1 QHIDINEPLSTLRDLLEPRLQIDLSKYEFWLQDNQMLDPDKSLVDQCVKGEGLVQINVQVISElgnEPRINILDILKPA 79

Sterile alpha motif (SAM)/Pointed domain of ETS-1; SAM Pointed domain of ETS-1 subfamily of ETS transcriptional activators is a protein-protein interaction domain. The ETS-1 activator is regulated by phosphorylation. It contains a docking site for the ERK2 MAP (Mitogen Activated Protein) kinase, while the ERK2 phosphorylation site is located in the N-terminal disordered region upstream of the SAM Pointed domain. Mutations of the kinase docking site residues inhibit phosphorylation. ETS-1 activators play a role in a number of different physiological processes, and they are expressed during embryonic development, including blood vessel formation, hematopoietic, lymphoid, neuronal and osteogenic differentiation. The Ets-1 gene is a proto-oncogene involved in progression of different tumors (including breast cancer, meningioma, and prostate cancer). Members of this subfamily are potential molecular targets for selective cancer therapy.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 442621308 187 SKFKREQIRLKIPEAANEWTHAHVTYWLEWAVKQFELVGINMSDWQMNGQELCAMTHEEFNQKLPRDPGNIFWTHLQLLK 266
Cdd:cd08542    4 SGFTKEQQRLGIPKDPREWTETHVREWVMWAVNEFSLKGVDFQKFCMSGAALCALGKECFLELAPDFVGDILWEHLEILQ 83

                 .
gi 442621308 267 E 267
Cdd:cd08542   84 K 84

Sterile alpha motif (SAM)/Pointed domain of ETS-2; SAM Pointed domain of ETS-2 subfamily of ETS transcriptional regulators is a protein-protein interaction domain. It contains a docking site for Cdk10 (cyclin-dependent kinase 10), a member of the Cdc2 kinase family. The interaction between ETS-2 and Cdk10 kinase inhibits ETS-2 transactivation activity in mammals. ETS-2 is also regulated by ERK2 MAP kinase. ETS-2, which is phosphorylated by ERK2, can interact with coactivators and enhance transactivation. ETS-2 transcriptional activators are involved in embryonic development and cell cycle control. The Ets-2 gene is a proto-oncogene. It is overexpressed in breast and prostate cancer cells and its overexpression is necessary for transformation of such cells. Members of ETS-2 subfamily are potential molecular targets for selective cancer therapy.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 442621308 187 SKFKREQIRLKIPEAANEWTHAHVTYWLEWAVKQFELVGINMSDWQMNGQELCAMTHEEFNQKLPRDPGNIFWTHL-QLL 265
Cdd:cd08543    4 SGFSKEQRRLGIPKNPWLWTEQQVCQWLLWATNEFSLVNVNFQQFLMNGQELCNLGKERFLELAPDFVGDILWEHLeQMI 83

                 ...
gi 442621308 266 KEC 268
Cdd:cd08543   84 KEN 86

Sterile alpha motif (SAM)/Pointed domain of ERG (Ets related gene) and FLI-1 (Friend leukemia integration 1) transcription factors; SAM Pointed domain of ERG/FLI-1 subfamily of ETS transcriptional regulators is a putative protein-protein interaction domain. The ERG and FLI regulators are involved in endothelial cell differentiation, bone morphogenesis and neural crest development. They are proto-oncogenes implicated in cancer development such as myeloid leukemia, Ewing's sarcoma and erythroleukemia. Members of this subfamily are potential targets for cancer therapy.

                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 442621308 198 IPEAANEWTHAHVTYWLEWAVKQFELVGINMSDWQ-MNGQELCAMTHEEFNQKLPRDPGNIFWTHLQLLKE 267
Cdd:cd08531    1 VPADPLLWTREHVRQWVEWAIKEYSLKDVDVSRFDnIDGKELCRMTRDDFLRLTSAYNGDVLISHLCYLRQ 71

Sterile alpha motif (SAM)/Pointed domain of prostate-derived ETS factor; SAM Pointed domain of PDEF-like (Prostate-Derived ETS Factor) subfamily of ETS transcriptional regulators is a putative protein-protein interaction domain. In human males this activator is highly expressed in the prostate gland and enhances androgen-mediated activation of the PSA promoter though interaction with the DNA binding domain of androgen receptor. PDEF may play a role in prostate cancer development as well as in goblet cell formation and mucus production in the epithelial lining of respiratory and intestinal tracts.

                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 442621308 196 LKIPEAANEWTHAHVTYWLEWAVKQFELVGINMSDWQMNGQELCAMTHEEFNQKLPRDpGNIFWTHLQLLK 266
Cdd:cd08532    8 LGISPDPYQWSTANVQAWLQWTEHQYRLPPNPPRCFQMNGKTLCALSEEDFVRRAPQG-GDTLHAQLEIWK 77

Sterile alpha motif (SAM)/Pointed domain of Tel/Yan protein; SAM Pointed domain of Tel (Translocation, Ets, Leukemia)/Yan subfamily of ETS transcriptional repressors is a protein-protein interaction domain. SAM Pointed domains of this type of regulators can interact with each other, forming head-to-tail homodimers or homooligomers, and/or interact with SAM Pointed domains of another subfamily of ETS factors forming heterodimers. The oligomeric form is able to block transcription of target genes and is involved in MAPK signaling. They participate in regulation of different processes during embryoniv development including hematopoietic differentiation and eye development. Tel/Yan transcriptional factors are frequent targets of chromosomal translocations resulting in fusions of SAM domain with new neighboring genes. Such chimeric proteins were found in different tumors. Members of this subfamily are potential targets for cancer therapy.

                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|..
gi 442621308 205 WTHAHVTYWLEWAVKQFELVGINMSDWQMNGQELCAMTHEEFNQKLPRdPGNIFWTHLQLLK 266
Cdd:cd08535    6 WSREDVLQWLRWAENEFSLPPIDSNTFEMNGKALCLLTKEDFRYRCPH-AGDVLYNLLQHLL 66

Sterile alpha motif (SAM)/Pointed domain of ESE-2 like ETS transcriptional regulators; SAM Pointed domain of ESE-2-like (Epithelium-Specific ETS) subfamily of ETS transcriptional regulators is a putative protein-protein interaction domain. It can act as a major transactivator by providing a potential docking site for co-activators. ESE-2 factors are involved in regulation of gene expression in a variety of epithelial (glandular and secretory) cells. ESE-2 mRNA was found in skin keratinocytes, salivary gland, mammary gland, stomach, prostate, and kidneys. The DNA binding consensus motif for ESE-2 consists of a GGA core and AT-rich flanks. The expression profiles of these factors are altered in epithelial cancers. Members of this subfamily are potential targets for cancer therapy.

                         10        20        30        40
                 ....*....|....*....|....*....|....*....|....
gi 442621308 205 WTHAHVTYWLEWAVKQFELVG--INMSDWQMNGQELCAMTHEEF 246
Cdd:cd08538   16 WTKRHVWEWLQFCCDQYKLDAncISFCHFNISGLQLCSMTQEEF 59

Sterile alpha motif (SAM)/Pointed domain of ESE-3 like ETS transcriptional regulators; SAM Pointed domain of ESE-3-like (Epithelium-Specific ETS) subfamily of ETS transcriptional regulators is a putative protein-protein interaction domain. It can act as a major transactivator by providing a potential docking site for co-activators. The ESE-3 transcriptional activator is involved in regulation of glandular epithelium differentiation through the MAP kinase signaling cascade. It is found to be expressed in glandular epithelium of prostate, pancreas, salivary gland, and trachea. Additionally, ESE-3 is differentially expressed during monocyte-derived dendritic cells development. DNA binding consensus motif for ESE-3 consists of purine-rich GGAA/T core sequence. The expression profiles of these factors are altered in epithelial cancers. Members of this subfamily are potential targets for cancer therapy.

                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 442621308 205 WTHAHVTYWLEWAV--KQFELVGINMSDWQMNGQELCAMTHEEFNQKlPRDPGNIFWTHLQLLK 266
Cdd:cd08539   12 WTKFQVWEWLQHLLdtNQLDANCIPFQEFDINGEHLCSMSLQEFTRA-AGTAGQLLYSNLQHLK 74