NCBI Home Page NCBI Site Search page NCBI Guide that lists and describes the NCBI resources
Conserved domains on  [gi|442620918|ref|NP_001262920|]
View 

uncharacterized protein Dmel_CG13623, isoform B [Drosophila melanogaster]

Protein Classification

iron-sulfur cluster assembly accessory protein( domain architecture ID 10015430)

iron-sulfur cluster assembly accessory protein from the HesB/IscA family is involved in the assembly of iron-sulfur proteins

Graphical summary

 Zoom to residue level

show extra options »

Show site features     Horizontal zoom: ×

List of domain hits

 Name Accession Description Interval E-value
TIGR00049 TIGR00049
Iron-sulfur cluster assembly accessory protein; Proteins in this subfamily appear to be ...
 36-137 2.22e-42

Iron-sulfur cluster assembly accessory protein; Proteins in this subfamily appear to be associated with the process of FeS-cluster assembly. The HesB proteins are associated with the nif gene cluster and the Rhizobium gene IscN has been shown to be required for nitrogen fixation. Nitrogenase includes multiple FeS clusters and many genes for their assembly. The E. coli SufA protein is associated with SufS, a NifS homolog and SufD which are involved in the FeS cluster assembly of the FhnF protein. The Azotobacter protein IscA (homologs of which are also found in E.coli) is associated which IscS, another NifS homolog and IscU, a nifU homolog as well as other factors consistent with a role in FeS cluster chemistry. A homolog from Geobacter contains a selenocysteine in place of an otherwise invariant cysteine, further suggesting a role in redox chemistry. [Biosynthesis of cofactors, prosthetic groups, and carriers, Other]


:

Pssm-ID: 272875 [Multi-domain]  Cd Length: 105  Bit Score: 135.79  E-value: 2.22e-42
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 442620918   36 QVSESCLKRLREICV----DGSFLRVTVEGGGCSGFQYKFDLDKQLNEDDRQFGEAEAKVVIDTVSLEYCSGATVDYHSE 111
Cdd:TIGR00049   1 TLTDSAAKRIKALLAgegePNLGLRVGVKGGGCSGLQYGLEFDDEPNEDDEVFEQDGVKVVVDPKSLPYLDGSEIDYVEE 80

                          90       100
                  ....*....|....*....|....*.
gi 442620918  112 LIRAGFRmVANPLAEQGCSCGSSFSI 137
Cdd:TIGR00049  81 LLGSGFT-FTNPNAKGTCGCGKSFSV 105
 
Name Accession Description Interval E-value
TIGR00049 TIGR00049
Iron-sulfur cluster assembly accessory protein; Proteins in this subfamily appear to be ...
 36-137 2.22e-42

Iron-sulfur cluster assembly accessory protein; Proteins in this subfamily appear to be associated with the process of FeS-cluster assembly. The HesB proteins are associated with the nif gene cluster and the Rhizobium gene IscN has been shown to be required for nitrogen fixation. Nitrogenase includes multiple FeS clusters and many genes for their assembly. The E. coli SufA protein is associated with SufS, a NifS homolog and SufD which are involved in the FeS cluster assembly of the FhnF protein. The Azotobacter protein IscA (homologs of which are also found in E.coli) is associated which IscS, another NifS homolog and IscU, a nifU homolog as well as other factors consistent with a role in FeS cluster chemistry. A homolog from Geobacter contains a selenocysteine in place of an otherwise invariant cysteine, further suggesting a role in redox chemistry. [Biosynthesis of cofactors, prosthetic groups, and carriers, Other]


Pssm-ID: 272875 [Multi-domain]  Cd Length: 105  Bit Score: 135.79  E-value: 2.22e-42
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 442620918   36 QVSESCLKRLREICV----DGSFLRVTVEGGGCSGFQYKFDLDKQLNEDDRQFGEAEAKVVIDTVSLEYCSGATVDYHSE 111
Cdd:TIGR00049   1 TLTDSAAKRIKALLAgegePNLGLRVGVKGGGCSGLQYGLEFDDEPNEDDEVFEQDGVKVVVDPKSLPYLDGSEIDYVEE 80

                          90       100
                  ....*....|....*....|....*.
gi 442620918  112 LIRAGFRmVANPLAEQGCSCGSSFSI 137
Cdd:TIGR00049  81 LLGSGFT-FTNPNAKGTCGCGKSFSV 105
PLN03082 PLN03082
Iron-sulfur cluster assembly; Provisional
 20-138 2.67e-37

Iron-sulfur cluster assembly; Provisional


Pssm-ID: 215564  Cd Length: 163  Bit Score: 124.65  E-value: 2.67e-37
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 442620918  20 MRHATASTSANPELS----VQVSESCLKRLREI-----CVDGSFLRVTVEGGGCSGFQYKFDLDKQLNEDDRQFGEAEAK 90
Cdd:PLN03082  36 LKEASTSSSSPSSSAsldaVHMTDNCIRRLKELqtsepSAEDKMLRLSVETGGCSGFQYVFELDDKTNSDDRVFEKDGVK 115

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....*...
gi 442620918  91 VVIDTVSLEYCSGATVDYHSELIRAGFRMVANPLAEQGCSCGSSFSIK 138
Cdd:PLN03082 116 LVVDNISYDFVKGATVDYVEELIRSAFVVSTNPSAVGGCSCKSSFMVK 163
IscA COG0316
Fe-S cluster assembly iron-binding protein IscA [Posttranslational modification, protein ...
 35-137 3.12e-35

Fe-S cluster assembly iron-binding protein IscA [Posttranslational modification, protein turnover, chaperones];


Pssm-ID: 440085 [Multi-domain]  Cd Length: 107  Bit Score: 117.56  E-value: 3.12e-35
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 442620918  35 VQVSESCLKRLREI----CVDGSFLRVTVEGGGCSGFQYKFDLDKQLNEDDRQFGEAEAKVVIDTVSLEYCSGATVDYHS 110
Cdd:COG0316    2 ITLTDAAAKRIKRLlakeGNPGLGLRVGVKGGGCSGFSYGLDFDDEPNEDDLVFEQDGVKVVVDPKSLPYLDGTEIDYVE 81

                         90       100
                 ....*....|....*....|....*..
gi 442620918 111 ELIRAGFRmVANPLAEQGCSCGSSFSI 137
Cdd:COG0316   82 ELLGSGFK-FNNPNAKSSCGCGESFSV 107
IscA_HesB_Se NF038090
IscA/HesB family protein; Members of this family, a large fraction of which are selenoproteins, ...
 35-131 1.13e-04

IscA/HesB family protein; Members of this family, a large fraction of which are selenoproteins, are homologous to proteins of iron-sulfur cluster biosynthesis such as IscA, and belong to the broader set of HesB-related proteins.


Pssm-ID: 411672  Cd Length: 99  Bit Score: 38.85  E-value: 1.13e-04
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 442620918  35 VQVSESCLKRLREICVDG---SFLRVTVEGGGCSGFQYKFDLDKQlNEDDRQFGEAEAKVVIDTVSLEYCSGATVDYHSE 111
Cdd:NF038090   2 LEVTDLASRKLKEYFADKkidSPIRVFLNQGGUAGPSLGLALDEP-KENDEVFEQDGLTFVIDKELLEQCGPIKVDFIDC 80

                         90       100
                 ....*....|....*....|.
gi 442620918 112 liRAGFRMV-ANPLAEQGCSC 131
Cdd:NF038090  81 --RSGFSITsSMPLGGGGCGS 99
 
Name Accession Description Interval E-value
TIGR00049 TIGR00049
Iron-sulfur cluster assembly accessory protein; Proteins in this subfamily appear to be ...
 36-137 2.22e-42

Iron-sulfur cluster assembly accessory protein; Proteins in this subfamily appear to be associated with the process of FeS-cluster assembly. The HesB proteins are associated with the nif gene cluster and the Rhizobium gene IscN has been shown to be required for nitrogen fixation. Nitrogenase includes multiple FeS clusters and many genes for their assembly. The E. coli SufA protein is associated with SufS, a NifS homolog and SufD which are involved in the FeS cluster assembly of the FhnF protein. The Azotobacter protein IscA (homologs of which are also found in E.coli) is associated which IscS, another NifS homolog and IscU, a nifU homolog as well as other factors consistent with a role in FeS cluster chemistry. A homolog from Geobacter contains a selenocysteine in place of an otherwise invariant cysteine, further suggesting a role in redox chemistry. [Biosynthesis of cofactors, prosthetic groups, and carriers, Other]


Pssm-ID: 272875 [Multi-domain]  Cd Length: 105  Bit Score: 135.79  E-value: 2.22e-42
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 442620918   36 QVSESCLKRLREICV----DGSFLRVTVEGGGCSGFQYKFDLDKQLNEDDRQFGEAEAKVVIDTVSLEYCSGATVDYHSE 111
Cdd:TIGR00049   1 TLTDSAAKRIKALLAgegePNLGLRVGVKGGGCSGLQYGLEFDDEPNEDDEVFEQDGVKVVVDPKSLPYLDGSEIDYVEE 80

                          90       100
                  ....*....|....*....|....*.
gi 442620918  112 LIRAGFRmVANPLAEQGCSCGSSFSI 137
Cdd:TIGR00049  81 LLGSGFT-FTNPNAKGTCGCGKSFSV 105
PLN03082 PLN03082
Iron-sulfur cluster assembly; Provisional
 20-138 2.67e-37

Iron-sulfur cluster assembly; Provisional


Pssm-ID: 215564  Cd Length: 163  Bit Score: 124.65  E-value: 2.67e-37
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 442620918  20 MRHATASTSANPELS----VQVSESCLKRLREI-----CVDGSFLRVTVEGGGCSGFQYKFDLDKQLNEDDRQFGEAEAK 90
Cdd:PLN03082  36 LKEASTSSSSPSSSAsldaVHMTDNCIRRLKELqtsepSAEDKMLRLSVETGGCSGFQYVFELDDKTNSDDRVFEKDGVK 115

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....*...
gi 442620918  91 VVIDTVSLEYCSGATVDYHSELIRAGFRMVANPLAEQGCSCGSSFSIK 138
Cdd:PLN03082 116 LVVDNISYDFVKGATVDYVEELIRSAFVVSTNPSAVGGCSCKSSFMVK 163
IscA COG0316
Fe-S cluster assembly iron-binding protein IscA [Posttranslational modification, protein ...
 35-137 3.12e-35

Fe-S cluster assembly iron-binding protein IscA [Posttranslational modification, protein turnover, chaperones];


Pssm-ID: 440085 [Multi-domain]  Cd Length: 107  Bit Score: 117.56  E-value: 3.12e-35
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 442620918  35 VQVSESCLKRLREI----CVDGSFLRVTVEGGGCSGFQYKFDLDKQLNEDDRQFGEAEAKVVIDTVSLEYCSGATVDYHS 110
Cdd:COG0316    2 ITLTDAAAKRIKRLlakeGNPGLGLRVGVKGGGCSGFSYGLDFDDEPNEDDLVFEQDGVKVVVDPKSLPYLDGTEIDYVE 81

                         90       100
                 ....*....|....*....|....*..
gi 442620918 111 ELIRAGFRmVANPLAEQGCSCGSSFSI 137
Cdd:COG0316   82 ELLGSGFK-FNNPNAKSSCGCGESFSV 107
PRK13623 PRK13623
iron-sulfur cluster insertion protein ErpA; Provisional
 55-137 1.37e-28

iron-sulfur cluster insertion protein ErpA; Provisional


Pssm-ID: 184186  Cd Length: 115  Bit Score: 101.16  E-value: 1.37e-28
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 442620918  55 LRVTVEGGGCSGFQYKFDLDKQLNEDDRQFGEAEAKVVIDTVSLEYCSGATVDYHSELIRAGFrMVANPLAEQGCSCGSS 134
Cdd:PRK13623  34 LRVYITGGGCSGFQYGFTFDEQVNEDDTTIEKQGVTLVVDPMSLQYLVGAEVDYTEGLEGSRF-VIKNPNAKTTCGCGSS 112


                 ...
gi 442620918 135 FSI 137
Cdd:PRK13623 113 FSI 115
iscA PRK09502
iron-sulfur cluster assembly protein IscA;
33-137 2.08e-13

iron-sulfur cluster assembly protein IscA;


Pssm-ID: 181914 [Multi-domain]  Cd Length: 107  Bit Score: 61.81  E-value: 2.08e-13
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 442620918  33 LSVQVSESCLKRLREICVD---GSFLRVTVEGGGCSGFQYKFDLDKQLNEDDRQFGEAEAKVVIDTVSLEYCSGATVDYH 109
Cdd:PRK09502   1 MSITLSDSAAARVNTFLANrgkGFGLRLGVRTSGCSGMAYVLEFVDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFV 80

                         90       100
                 ....*....|....*....|....*...
gi 442620918 110 SELIRAGFRMvANPLAEQGCSCGSSFSI 137
Cdd:PRK09502  81 KEGLNEGFKF-TNPNVKDECGCGESFHV 107
sufA PRK09504
iron-sulfur cluster assembly scaffold protein; Provisional
 55-137 4.13e-10

iron-sulfur cluster assembly scaffold protein; Provisional


Pssm-ID: 181915  Cd Length: 122  Bit Score: 53.59  E-value: 4.13e-10
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 442620918  55 LRVTVEGGGCSGFQYKFDLDKQLNEDDRQFGEAEAKVVIDTVSLEYCSGATVDYHSELIRAGFRMvANPLAEQGCSCGSS 134
Cdd:PRK09504  41 VRLGVKQTGCAGFGYVLDSVSEPDKDDLVFEHDGAKLFVPLQAMPFIDGTEVDYVREGLNQIFKF-HNPKAQNECGCGES 119


                 ...
gi 442620918 135 FSI 137
Cdd:PRK09504 120 FGV 122
IscA_HesB_Se NF038090
IscA/HesB family protein; Members of this family, a large fraction of which are selenoproteins, ...
 35-131 1.13e-04

IscA/HesB family protein; Members of this family, a large fraction of which are selenoproteins, are homologous to proteins of iron-sulfur cluster biosynthesis such as IscA, and belong to the broader set of HesB-related proteins.


Pssm-ID: 411672  Cd Length: 99  Bit Score: 38.85  E-value: 1.13e-04
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 442620918  35 VQVSESCLKRLREICVDG---SFLRVTVEGGGCSGFQYKFDLDKQlNEDDRQFGEAEAKVVIDTVSLEYCSGATVDYHSE 111
Cdd:NF038090   2 LEVTDLASRKLKEYFADKkidSPIRVFLNQGGUAGPSLGLALDEP-KENDEVFEQDGLTFVIDKELLEQCGPIKVDFIDC 80

                         90       100
                 ....*....|....*....|.
gi 442620918 112 liRAGFRMV-ANPLAEQGCSC 131
Cdd:NF038090  81 --RSGFSITsSMPLGGGGCGS 99
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
Help | Disclaimer | Write to the Help Desk
NCBI | NLM | NIH