RhoGAP_nadrin: RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain of Nadrin-like proteins. Nadrin, also named Rich-1, has been shown to be involved in the regulation of Ca2+-dependent exocytosis in neurons and recently has been implicated in tight junction maintenance in mammalian epithelium. Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 392926009 235 PRPVFGVPLDEHLSIQKERISGVLTKCCDFLRQNGMNERGIFRVSGNASKIKRIRAALDAGQFDADEKHYNNDPHAVAST 314
Cdd:cd04386    1 EKPVFGTPLEEHLKRTGREIALPIEACVMCLLETGMNEEGLFRVGGGASKLKRLKAALDAGTFSLPLDEFYSDPHAVASA 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 392926009 315 LKAYLRELPDPLTMDSLQSDWVEAINLEGE-ERFSAIDRCLKKMTRGHRQNLIYLMKFLCDLEAKREETSMNASNLAIVF 393
Cdd:cd04386   81 LKSYLRELPDPLLTYNLYEDWVQAANKPDEdERLQAIWRILNKLPRENRDNLRYLIKFLSKLAQKSDENKMSPSNIAIVL 160

                        170       180       190       200
                 ....*....|....*....|....*....|....*....|...
gi 392926009 394 APTMTGMIYD--------GMNTHGVKLTEFMISNGVRIFNFGA 428
Cdd:cd04386  161 APNLLWAKNEgslaemaaGTSVHVVAIVELIISHADWFFPGEV 203

RhoGAP_nadrin: RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain of Nadrin-like proteins. Nadrin, also named Rich-1, has been shown to be involved in the regulation of Ca2+-dependent exocytosis in neurons and recently has been implicated in tight junction maintenance in mammalian epithelium. Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 392926009 235 PRPVFGVPLDEHLSIQKERISGVLTKCCDFLRQNGMNERGIFRVSGNASKIKRIRAALDAGQFDADEKHYNNDPHAVAST 314
Cdd:cd04386    1 EKPVFGTPLEEHLKRTGREIALPIEACVMCLLETGMNEEGLFRVGGGASKLKRLKAALDAGTFSLPLDEFYSDPHAVASA 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 392926009 315 LKAYLRELPDPLTMDSLQSDWVEAINLEGE-ERFSAIDRCLKKMTRGHRQNLIYLMKFLCDLEAKREETSMNASNLAIVF 393
Cdd:cd04386   81 LKSYLRELPDPLLTYNLYEDWVQAANKPDEdERLQAIWRILNKLPRENRDNLRYLIKFLSKLAQKSDENKMSPSNIAIVL 160

                        170       180       190       200
                 ....*....|....*....|....*....|....*....|...
gi 392926009 394 APTMTGMIYD--------GMNTHGVKLTEFMISNGVRIFNFGA 428
Cdd:cd04386  161 APNLLWAKNEgslaemaaGTSVHVVAIVELIISHADWFFPGEV 203

GTPase-activator protein for Rho-like GTPases; GTPase activator proteins towards Rho/Rac/Cdc42-like small GTPases. etter domain limits and outliers.

                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 392926009   257 VLTKCCDFLRQNGMNERGIFRVSGNASKIKRIRAALDAGQfDADEKHYNNDPHAVASTLKAYLRELPDPLTMDSLQSDWV 336
Cdd:smart00324   6 IVEKCIEYLEKRGLDTEGIYRVSGSKSRVKELRDAFDSGP-DPDLDLSEYDVHDVAGLLKLFLRELPEPLITYELYEEFI 84

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 392926009   337 EAINLEGE-ERFSAIDRCLKKMTRGHRQNLIYLMKFLCDLEAKREETSMNASNLAIVFAPTMTGMIYDGM-----NTHGV 410
Cdd:smart00324  85 EAAKLEDEtERLRALRELLSLLPPANRATLRYLLAHLNRVAEHSEENKMTARNLAIVFGPTLLRPPDGEVaslkdIRHQN 164

                   ....*....
gi 392926009   411 KLTEFMISN 419
Cdd:smart00324 165 TVIEFLIEN 173

RhoGAP_nadrin: RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain of Nadrin-like proteins. Nadrin, also named Rich-1, has been shown to be involved in the regulation of Ca2+-dependent exocytosis in neurons and recently has been implicated in tight junction maintenance in mammalian epithelium. Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 392926009 235 PRPVFGVPLDEHLSIQKERISGVLTKCCDFLRQNGMNERGIFRVSGNASKIKRIRAALDAGQFDADEKHYNNDPHAVAST 314
Cdd:cd04386    1 EKPVFGTPLEEHLKRTGREIALPIEACVMCLLETGMNEEGLFRVGGGASKLKRLKAALDAGTFSLPLDEFYSDPHAVASA 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 392926009 315 LKAYLRELPDPLTMDSLQSDWVEAINLEGE-ERFSAIDRCLKKMTRGHRQNLIYLMKFLCDLEAKREETSMNASNLAIVF 393
Cdd:cd04386   81 LKSYLRELPDPLLTYNLYEDWVQAANKPDEdERLQAIWRILNKLPRENRDNLRYLIKFLSKLAQKSDENKMSPSNIAIVL 160

                        170       180       190       200
                 ....*....|....*....|....*....|....*....|...
gi 392926009 394 APTMTGMIYD--------GMNTHGVKLTEFMISNGVRIFNFGA 428
Cdd:cd04386  161 APNLLWAKNEgslaemaaGTSVHVVAIVELIISHADWFFPGEV 203

RhoGAP domain; GTPase activator proteins towards Rho/Rac/Cdc42-like small GTPases.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 392926009  257 VLTKCCDFLRQNGMNERGIFRVSGNASKIKRIRAALDAGQfDADEKHYNNDPHAVASTLKAYLRELPDPLTMDSLQSDWV 336
Cdd:pfam00620   3 IVRKCVEYLEKRGLDTEGIFRVSGSASRIKELREAFDRGP-DVDLDLEEEDVHVVASLLKLFLRELPEPLLTFELYEEFI 81

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|..
gi 392926009  337 EAINLEGE-ERFSAIDRCLKKMTRGHRQNLIYLMKFLCDLEAKREETSMNASNLAIVFAPTM 397
Cdd:pfam00620  82 EAAKLPDEeERLEALRELLRKLPPANRDTLRYLLAHLNRVAQNSDVNKMNAHNLAIVFGPTL 143

RhoGAP_CdGAP: RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain of CdGAP-like proteins; CdGAP contains an N-terminal RhoGAP domain and a C-terminal proline-rich region, and it is active on both Cdc42 and Rac1 but not RhoA. CdGAP is recruited to focal adhesions via the interaction with the scaffold protein actopaxin (alpha-parvin). Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 392926009 237 PVFGVPLDEHLSIQKERISGVLTKCCDFLRQNGMNErGIFRVSGNASKIKRIRAALDAGQF-DADEKHYNNDPHAVASTL 315
Cdd:cd04384    1 RVFGCDLTEHLLNSGQDVPQVLKSCTEFIEKHGIVD-GIYRLSGIASNIQRLRHEFDSEQIpDLTKDVYIQDIHSVSSLC 79

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 392926009 316 KAYLRELPDPLTMDSLQSDWVEAINL-EGEERFSAIDRCLKKMTRGHRQNLIYLMKFLCDLEAKREETSMNASNLAIVFA 394
Cdd:cd04384   80 KLYFRELPNPLLTYQLYEKFSEAVSAaSDEERLEKIHDVIQQLPPPHYRTLEFLMRHLSRLAKYCSITNMHAKNLAIVWA 159

                 ...
gi 392926009 395 PTM 397
Cdd:cd04384  160 PNL 162

RhoGAP_fRGD1: RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain of fungal RGD1-like proteins. Yeast Rgd1 is a GAP protein for Rho3 and Rho4 and plays a role in low-pH response. Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 392926009 239 FGVPLDEHLSIQKERISGVLTKCCDFLRQNGMNERGIFRVSGNASKIKRIRAALDA--GQFDADEK-HYNNDPHAVASTL 315
Cdd:cd04398    1 FGVPLEDLILREGDNVPNIVYQCIQAIENFGLNLEGIYRLSGNVSRVNKLKELFDKdpLNVLLISPeDYESDIHSVASLL 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 392926009 316 KAYLRELPDPLTMDSLQSDWVEAINLEGE-ERFSAIDRCLKKMTRGHRQNLIYLMKFLCDLEAKREETSMNASNLAIVFA 394
Cdd:cd04398   81 KLFFRELPEPLLTKALSREFIEAAKIEDEsRRRDALHGLINDLPDANYATLRALMFHLARIKEHESVNRMSVNNLAIIWG 160

                        170       180       190
                 ....*....|....*....|....*....|..
gi 392926009 395 PTMTGMIYDGMNTHG--VKLTEFMISNGVRIF 424
Cdd:cd04398  161 PTLMNAAPDNAADMSfqSRVIETLLDNAYQIF 192

RhoGAP-p50rhoGAP: RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain of p50RhoGAP-like proteins; p50RhoGAP, also known as RhoGAP-1, contains a C-terminal RhoGAP domain and an N-terminal Sec14 domain which binds phosphatidylinositol 3,4,5-trisphosphate (PtdIns(3,4,5)P3). It is ubiquitously expressed and preferentially active on Cdc42. This subgroup also contains closely related ARHGAP8. Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 392926009 234 LPRPVFGVPLdEHL---SIQKERISGVLTKCCDFLRQNGMNERGIFRVSGNASKIKRIRAALDAGQfDADEKHYNnDPHA 310
Cdd:cd04404    1 LPTQQFGVSL-QFLkekNPEQEPIPPVVRETVEYLQAHALTTEGIFRRSANTQVVKEVQQKYNMGE-PVDFDQYE-DVHL 77

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 392926009 311 VASTLKAYLRELPDPLTMDSLQSDWVEAINLEGEERFSAIDRCLKKMTRGHRQNLIYLMKFLCDLEAKREETSMNASNLA 390
Cdd:cd04404   78 PAVILKTFLRELPEPLLTFDLYDDIVGFLNVDKEERVERVKQLLQTLPEENYQVLKYLIKFLVQVSAHSDQNKMTNSNLA 157

                 ....*...
gi 392926009 391 IVFAPTMT 398
Cdd:cd04404  158 VVFGPNLL 165

RhoGAP_ARHGAP6: RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain of ArhGAP6-like proteins. ArhGAP6 shows GAP activity towards RhoA, but not towards Cdc42 and Rac1. ArhGAP6 is often deleted in microphthalmia with linear skin defects syndrome (MLS); MLS is a severe X-linked developmental disorder. Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 392926009 252 ERISGVLTKCCDFLRQNGMNERGIFRVSGNASKIKRIRAALDAGQFDA-DEKHynnDPHAVASTLKAYLRELPDPLTMDS 330
Cdd:cd04376    7 RQVPRLVESCCQHLEKHGLQTVGIFRVGSSKKRVRQLREEFDRGIDVVlDENH---SVHDVAALLKEFFRDMPDPLLPRE 83

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 392926009 331 LQSDWVEAINLEGEERFSAIDRCLKKMTRGHRQNLIYLMKFLCDLEAKREET-----------SMNASNLAIVFAPTMTG 399
Cdd:cd04376   84 LYTAFIGTALLEPDEQLEALQLLIYLLPPCNCDTLHRLLKFLHTVAEHAADSidedgqevsgnKMTSLNLATIFGPNLLH 163

                        170       180       190
                 ....*....|....*....|....*....|....*..
gi 392926009 400 MIYDGM------------NTHGVKLTEFMISNGVRIF 424
Cdd:cd04376  164 KQKSGErefvqaslrieeSTAIINVVQTMIDNYEELF 200

RhoGAP_fBEM3: RhoGAP (GTPase-activator [GAP] protein for Rho-like small GTPases) domain of fungal BEM3-like proteins. Bem3 is a GAP protein of Cdc42, and is specifically involved in the control of the initial assembly of the septin ring in yeast bud formation. Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 392926009 238 VFGVPLDEHLSIQKERISG-----VLTKCCDFL-RQNGMNERGIFRVSGNASKIKRIRAALDAGqFDADEKHYNN--DPH 309
Cdd:cd04400    1 IFGSPLEEAVELSSHKYNGrdlpsVVYRCIEYLdKNRAIYEEGIFRLSGSASVIKQLKERFNTE-YDVDLFSSSLypDVH 79

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 392926009 310 AVASTLKAYLRELPDPLTMDSLQSDWVEAINLEGE--ERFSAIDRCLKKMTRGHRQNLIYLMKFLCDLEAKREETSMNAS 387
Cdd:cd04400   80 TVAGLLKLYLRELPTLILGGELHNDFKRLVEENHDrsQRALELKDLVSQLPQANYDLLYVLFSFLRKIIEHSDVNKMNLR 159

                        170
                 ....*....|
gi 392926009 388 NLAIVFAPTM 397
Cdd:cd04400  160 NVCIVFSPTL 169

RhoGAP_p190: RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain of p190-like proteins. p190, also named RhoGAP5, plays a role in neuritogenesis and axon branch stability. p190 shows a preference for Rho, over Rac and Cdc42, and consists of an N-terminal GTPase domain and a C-terminal GAP domain. The central portion of p190 contains important regulatory phosphorylation sites. Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 392926009 239 FGVPLDEHLSIQKErISGVLTKCCDFLRQNGMNERGIFRVSGNASKIKRIRAALDA-GQFDADEKHYNndPHAVASTLKA 317
Cdd:cd04373    1 FGVPLANVVTSEKP-IPIFLEKCVEFIEATGLETEGIYRVSGNKTHLDSLQKQFDQdHNLDLVSKDFT--VNAVAGALKS 77

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 392926009 318 YLRELPDPLTMDSLQSDWVEAINL-EGEERFSAIDRCLKKMTRGHRQNLIYLMKFLCDLEAKREETSMNASNLAIVFAPT 396
Cdd:cd04373   78 FFSELPDPLIPYSMHLELVEAAKInDREQRLHALKELLKKFPPENFDVFKYVITHLNKVSQNSKVNLMTSENLSICFWPT 157

                 .
gi 392926009 397 M 397
Cdd:cd04373  158 L 158

RhoGAP_ARHGAP22_24_25: GTPase-activator protein (GAP) domain for Rho-like GTPases found in ARHGAP22, 24 and 25-like proteins; longer isoforms of these proteins contain an additional N-terminal pleckstrin homology (PH) domain. ARHGAP25 (KIA0053) has been identified as a GAP for Rac1 and Cdc42. Short isoforms (without the PH domain) of ARHGAP24, called RC-GAP72 and p73RhoGAP, and of ARHGAP22, called p68RacGAP, has been shown to be involved in angiogenesis and endothelial cell capillary formation. Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 392926009 238 VFGVPLDEhlSIQKERISGV------LTKCCDFLRQNGMNERGIFRVSGNASKIKRIRAALDAGQ---FDADekhynNDP 308
Cdd:cd04390    2 VFGQRLED--TVAYERKFGPrlvpilVEQCVDFIREHGLKEEGLFRLPGQANLVKQLQDAFDAGErpsFDSD-----TDV 74

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 392926009 309 HAVASTLKAYLRELPDPLTMDSLQSDWVEAINLEGEERFSAIDRCLKKMTRGHRQN---LIYLMKFLCDLEAKREETSMN 385
Cdd:cd04390   75 HTVASLLKLYLRELPEPVIPWAQYEDFLSCAQLLSKDEEKGLGELMKQVSILPKVNynlLSYICRFLDEVQSNSSVNKMS 154

                        170
                 ....*....|..
gi 392926009 386 ASNLAIVFAPTM 397
Cdd:cd04390  155 VQNLATVFGPNI 166

RhoGAP_Bcr: RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain of Bcr (breakpoint cluster region protein)-like proteins. Bcr is a multidomain protein with a variety of enzymatic functions. It contains a RhoGAP and a Rho GEF domain, a Ser/Thr kinase domain, an N-terminal oligomerization domain, and a C-terminal PDZ binding domain, in addition to PH and C2 domains. Bcr is a negative regulator of: i) RacGTPase, via the Rho GAP domain, ii) the Ras-Raf-MEK-ERK pathway, via phosphorylation of the Ras binding protein AF-6, and iii) the Wnt signaling pathway through binding beta-catenin. Bcr can form a complex with beta-catenin and Tcf1. The Wnt signaling pathway is involved in cell proliferation, differentiation, and cell renewal. Bcr was discovered as a fusion partner of Abl. The Bcr-Abl fusion is characteristic for a large majority of chronic myelogenous leukemias (CML). Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 392926009 239 FGVPLDEHLSIQKERISGVLTKCCDFLRQNGMNERGIFRVSGNASKIKRIRAALDAGQFDADEKHYNNDPHAVASTLKAY 318
Cdd:cd04387    1 FGVKISTVTKRERSKVPYIVRQCVEEVERRGMEEVGIYRISGVATDIQALKAAFDTNNKDVSVMLSEMDVNAIAGTLKLY 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 392926009 319 LRELPDPLTMDSLQSDWVEAINL-EGEERFSAIDRCLKKMTRGHRQNLIYLMKFLCDLEAKREETSMNASNLAIVFAPTM 397
Cdd:cd04387   81 FRELPEPLFTDELYPNFAEGIALsDPVAKESCMLNLLLSLPDPNLVTFLFLLHHLKRVAEREEVNKMSLHNLATVFGPTL 160

RhoGAP_KIAA1688: GTPase-activator protein (GAP) domain for Rho-like GTPases found in KIAA1688-like proteins; KIAA1688 is a protein of unknown function that contains a RhoGAP domain and a myosin tail homology 4 (MyTH4) domain. Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 392926009 239 FGVPLDEHLSIQKE-----RISGVLTKCCDFLRQ-NGMNERGIFRVSGNASKIKRIRAALDAGQFDADEKHynnDPHAVA 312
Cdd:cd04389    1 FGSSLEEIMDRQKEkypelKLPWILTFLSEKVLAlGGFQTEGIFRVPGDIDEVNELKLRVDQWDYPLSGLE---DPHVPA 77

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 392926009 313 STLKAYLRELPDPLTMDSLQSDWVEAinlegEERFSAIDRCLKKMTRGHRQNLIYLMKFL--CDLEAKREETSMNASNLA 390
Cdd:cd04389   78 SLLKLWLRELEEPLIPDALYQQCISA-----SEDPDKAVEIVQKLPIINRLVLCYLINFLqvFAQPENVAHTKMDVSNLA 152

                 ....*
gi 392926009 391 IVFAP 395
Cdd:cd04389  153 MVFAP 157

RhoGAP_myosin_IXB: RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain present in myosins IXB. Class IX myosins contain a characteristic head domain, a neck domain and a tail domain which contains a C6H2-zinc binding motif and a Rho-GAP domain. Class IX myosins are single-headed, processive myosins that are partly cytoplasmic, and partly associated with membranes and the actin cytoskeleton. Class IX myosins are implicated in the regulation of neuronal morphogenesis and function of sensory systems, like the inner ear. There are two major isoforms, myosin IXA and IXB with several splice variants, which are both expressed in developing neurons Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 392926009 239 FGVPLDEhLSIQKERISGVLTKCCDFLRQNGMNERGIFRVSGNASKIKRIRAALDAGQfdADEKHYNNDPHAVASTLKAY 318
Cdd:cd04407    1 FGVRVGS-LTSNKTSVPIVLEKLLEHVEMHGLYTEGIYRKSGSANRMKELHQLLQADP--ENVKLENYPIHAITGLLKQW 77

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 392926009 319 LRELPDPLTMDSLQSDWVEAINL-EGEERFSAIDRCLKKMTRGHRQNLIYLMKFLCDLEAKREETSMNASNLAIVFAPTM 397
Cdd:cd04407   78 LRELPEPLMTFAQYNDFLRAVELpEKQEQLQAIYRVLEQLPTANHNTLERLIFHLVKVALEEDVNRMSPNALAIVFAPCL 157

The Bin/Amphiphysin/Rvs (BAR) domain of Rich-like Rho GTPase Activating Proteins; BAR domains are dimerization, lipid binding and curvature sensing modules found in many different proteins with diverse functions. This subfamily is composed of Rho and Rac GTPase activating proteins (GAPs) with similarity to GAP interacting with CIP4 homologs proteins (Rich). Members contain an N-terminal BAR domain, followed by a Rho GAP domain, and a C-terminal prolin-rich region. Vertebrates harbor at least three Rho GAPs in this subfamily including Rich1, Rich2, and SH3-domain binding protein 1 (SH3BP1). Rich1 and Rich2 play complementary roles in the establishment and maintenance of cell polarity. Rich1 is a Cdc42- and Rac-specific GAP that binds to polarity proteins through the scaffold protein angiomotin and plays a role in maintaining the integrity of tight junctions. Rich2 is a Rac GAP that interacts with CD317 and plays a role in actin cytoskeleton organization and the maintenance of microvilli in polarized epithelial cells. SH3BP1 is a Rac GAP that inhibits Rac-mediated platelet-derived growth factor (PDGF)-induced membrane ruffling. BAR domains form dimers that bind to membranes, induce membrane bending and curvature, and may also be involved in protein-protein interactions. The BAR domain of Rich1 has been shown to form oligomers, bind membranes and induce membrane tubulation.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 392926009  58 EENVDKRKKKVEELTMAQNLREASVDLDyfQTSCLHRVINAYGTVLHAEVDEKVKLEMAMERRVYEELAPFSEYE-KNVN 136
Cdd:cd07595   47 GEDKDKRLKKLPEYGLAQSMLESSKELP--DDSLLGKVLKLCGEAQNTLARELVDHEMNVEEDVLSPLQNILEVEiPNIQ 124

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 392926009 137 KLKDKLNLVSTDLEIAQKDLEKENSMTHQ-----------ESLDAIQMKYEGMKDALVTDIFaSSVSKEFQLNEHMLTSM 205
Cdd:cd07595  125 KQKKRLSKLVLDMDSARSRYNAAHKSSGGqgaaakvdalkDEYEEAELKLEQCRDALATDMY-EFLAKEAEIASYLIDLI 203

                        170       180       190       200
                 ....*....|....*....|....*....|....*....|.
gi 392926009 206 NLKRDYFKKMYEMYEKAIPEVERMIATALPRPVFGVPLDEH 246
Cdd:cd07595  204 EAQREYHRTALSVLEAVLPELQEQIEQSPSKPVFGQPLEEH 244

RhoGAP_DLC1: RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain of DLC1-like proteins. DLC1 shows in vitro GAP activity towards RhoA and CDC42. Beside its C-terminal GAP domain, DLC1 also contains a SAM (sterile alpha motif) and a START (StAR-related lipid transfer action) domain. DLC1 has tumor suppressor activity in cell culture. Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 392926009 236 RPVFGVPLDEHLSIQKERISGVLTKCCDFLRQNGMNERGIFRVSGNASKIKRIRAALDAgqfDADEKHYNN-DPHAVAST 314
Cdd:cd04375    2 KNVFGVPLLVNLQRTGQPLPRSIQQAMRWLRNNALDQVGLFRKSGVKSRIQKLRSMIES---STDNVNYDGqQAYDVADM 78

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 392926009 315 LKAYLRELPDPLtMDSLQSDWVEAI--NLEGEERFSAIDRCLKKMTRGHRQNLIYLMKFLCDLEAKREETSMNASNLAIV 392
Cdd:cd04375   79 LKQYFRDLPEPL-LTNKLSETFIAIfqYVPKEQRLEAVQCAILLLPDENREVLQTLLYFLSDVAANSQENQMTATNLAVC 157

                 ....*
gi 392926009 393 FAPTM 397
Cdd:cd04375  158 LAPSL 162

RhoGAP_srGAP: RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain present in srGAPs. srGAPs are components of the intracellular part of Slit-Robo signalling pathway that is important for axon guidance and cell migration. srGAPs contain an N-terminal FCH domain, a central RhoGAP domain and a C-terminal SH3 domain; this SH3 domain interacts with the intracellular proline-rich-tail of the Roundabout receptor (Robo). This interaction with Robo then activates the rhoGAP domain which in turn inhibits Cdc42 activity. Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 392926009 239 FGVPLDEHLSIQKERISGVLTKCCDFLRQNGMNERGIFRVSGNASKIKRIRAALDAGQFDADEKHYNNDPHAVASTLKAY 318
Cdd:cd04383    3 FNGSLEEYIQDSGQAIPLVVESCIRFINLYGLQHQGIFRVSGSQVEVNDIKNAFERGEDPLADDQNDHDINSVAGVLKLY 82

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 392926009 319 LRELPDPLTMDSLQSDWVEAINLEGE-ERFSAIDRCLKKMTRGHRQNLIYLMKFLCDLEAKREETSMNASNLAIVFAPTM 397
Cdd:cd04383   83 FRGLENPLFPKERFEDLMSCVKLENPtERVHQIREILSTLPRSVIIVMRYLFAFLNHLSQFSDENMMDPYNLAICFGPTL 162

RhoGAP_myosin_IXA: RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain present in myosins IXA. Class IX myosins contain a characteristic head domain, a neck domain and a tail domain which contains a C6H2-zinc binding motif and a Rho-GAP domain. Class IX myosins are single-headed, processive myosins that are partly cytoplasmic, and partly associated with membranes and the actin cytoskeleton. Class IX myosins are implicated in the regulation of neuronal morphogenesis and function of sensory systems, like the inner ear. There are two major isoforms, myosin IXA and IXB with several splice variants, which are both expressed in developing neurons. Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 392926009 239 FGVPLDEhLSIQKERISGVLTKCCDFLRQNGMNERGIFRVSGNASKIKRIRAALDAGQFDADEKHYNndPHAVASTLKAY 318
Cdd:cd04406    1 FGVELSR-LTSEDRSVPLVVEKLINYIEMHGLYTEGIYRKSGSTNKIKELRQGLDTDANSVNLDDYN--IHVIASVFKQW 77

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 392926009 319 LRELPDPLTMDSLQSDWVEAINL-EGEERFSAIDRCLKKMTRGHRQNLIYLMKFLCDLEAKREETSMNASNLAIVFAPTM 397
Cdd:cd04406   78 LRDLPNPLMTFELYEEFLRAMGLqERRETVRGVYSVIDQLSRTHLNTLERLIFHLVRIALQEETNRMSANALAIVFAPCI 157

RhoGAP_GMIP: RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain of GMIP (Gem interacting protein). GMIP plays important roles in neurite growth and axonal guidance, and interacts with Gem, a member of the RGK subfamily of the Ras small GTPase superfamily, through the N-terminal half of the protein. GMIP contains a C-terminal RhoGAP domain. GMIP inhibits RhoA function, but is inactive towards Rac1 and Cdc41. Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 392926009 252 ERISGVLTKCCDFLRQNGMNERGIFRVSGNASKIKRIRAALDAGQFDADEKhyNNDPHAVASTLKAYLRELPDPLTM--- 328
Cdd:cd04408   14 EEVPFVVVRCTAEIENRALGVQGIYRISGSKARVEKLCQAFENGRDLVDLS--GHSPHDITSVLKHFLKELPEPVLPfql 91