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Conserved domains on  [gi|392921807|ref|NP_001256577|]
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MFS domain-containing protein [Caenorhabditis elegans]

Protein Classification

MFS transporter( domain architecture ID 999995)

major facilitator superfamily (MFS) transporter facilitates the transport across cytoplasmic or internal membranes of one or more from a variety of substrates including ions, sugar phosphates, drugs, neurotransmitters, nucleosides, amino acids, and peptides

CATH:  1.20.1250.20
Gene Ontology:  GO:0022857|GO:0055085
PubMed:  26758938|26098515
SCOP:  3000310
TCDB:  2.A.1

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
MFS super family cl28910
Major Facilitator Superfamily; The Major Facilitator Superfamily (MFS) is a large and diverse ...
 57-147 3.01e-22

Major Facilitator Superfamily; The Major Facilitator Superfamily (MFS) is a large and diverse group of secondary transporters that includes uniporters, symporters, and antiporters. MFS proteins facilitate the transport across cytoplasmic or internal membranes of a variety of substrates including ions, sugar phosphates, drugs, neurotransmitters, nucleosides, amino acids, and peptides. They do so using the electrochemical potential of the transported substrates. Uniporters transport a single substrate, while symporters and antiporters transport two substrates in the same or in opposite directions, respectively, across membranes. MFS proteins are typically 400 to 600 amino acids in length, and the majority contain 12 transmembrane alpha helices (TMs) connected by hydrophilic loops. The N- and C-terminal halves of these proteins display weak similarity and may be the result of a gene duplication/fusion event. Based on kinetic studies and the structures of a few bacterial superfamily members, GlpT (glycerol-3-phosphate transporter), LacY (lactose permease), and EmrD (multidrug transporter), MFS proteins are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement. Bacterial members function primarily for nutrient uptake, and as drug-efflux pumps to confer antibiotic resistance. Some MFS proteins have medical significance in humans such as the glucose transporter Glut4, which is impaired in type II diabetes, and glucose-6-phosphate transporter (G6PT), which causes glycogen storage disease when mutated.


The actual alignment was detected with superfamily member cd17326:

Pssm-ID: 475125 [Multi-domain]  Cd Length: 374  Bit Score: 90.73  E-value: 3.01e-22
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 392921807  57 VYLICFIVFFGIAFPFVESPSAALYSEILGPRKQGNMQGLFSLGGSLAPVIGSLSSTALFQASGFRYVMVYQAVVLVIGA 136
Cdd:cd17326  284 PWGITGVILFGLGYPIGNVMLYTLYSKILGPKPQGTMQGVLTASGSLARTLGPIFVTAIYTHFGPRWTWIITLGLLLLAI 363

                         90
                 ....*....|.
gi 392921807 137 LLIGVFYKRLV 147
Cdd:cd17326  364 ILLIVFYKRLV 374
 
Name Accession Description Interval E-value
MFS_MFSD8 cd17326
Major facilitator superfamily domain-containing protein 8; Major facilitator superfamily (MFS) ...
 57-147 3.01e-22

Major facilitator superfamily domain-containing protein 8; Major facilitator superfamily (MFS) domain-containing protein 8 (MFSD8) is also called ceroid-lipofuscinosis neuronal protein 7 (CLN7). It is a polytopic lysosomal membrane protein that may transport small solutes by using chemiosmotic ion gradients. Mutations in MFSD8/CLN7 cause a variant of late-infantile neuronal ceroid lipofuscinoses (vLINCL), a neurodegenerative lysosomal storage disorder. Some variants are associated with nonsyndromic autosomal recessive macular dystrophy. MFSD8/CLN7 belongs to the Eukaryotic Solute carrier 46 (SLC46)/Bacterial Tetracycline resistance -like (SLC46/TetA-like) family of the Major Facilitator Superfamily (MFS) of transporters. MFS proteins are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.


Pssm-ID: 340884 [Multi-domain]  Cd Length: 374  Bit Score: 90.73  E-value: 3.01e-22
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 392921807  57 VYLICFIVFFGIAFPFVESPSAALYSEILGPRKQGNMQGLFSLGGSLAPVIGSLSSTALFQASGFRYVMVYQAVVLVIGA 136
Cdd:cd17326  284 PWGITGVILFGLGYPIGNVMLYTLYSKILGPKPQGTMQGVLTASGSLARTLGPIFVTAIYTHFGPRWTWIITLGLLLLAI 363

                         90
                 ....*....|.
gi 392921807 137 LLIGVFYKRLV 147
Cdd:cd17326  364 ILLIVFYKRLV 374
UhpC COG2271
Sugar phosphate permease [Carbohydrate transport and metabolism];
53-146 5.26e-05

Sugar phosphate permease [Carbohydrate transport and metabolism];


Pssm-ID: 441872 [Multi-domain]  Cd Length: 363  Bit Score: 41.78  E-value: 5.26e-05
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 392921807  53 VPLPVYLICFIVFFGIAFPFVESPSAALYSEILGPRKQGNMQGLFSLGGSLAPVIGSLSSTALFQASGFRYVMVYQAVVL 132
Cdd:COG2271  268 LPSPALAIALLFLAGFGLGGAFGLLWALAAELFPKKARGTASGLVNTFGFLGGALGPLLVGYLLDATGYQAAFLLLAALA 347

                         90
                 ....*....|....
gi 392921807 133 VIGALLIGVFYKRL 146
Cdd:COG2271  348 LLAALLALLLLRET 361
MFS_1 pfam07690
Major Facilitator Superfamily;
59-157 4.38e-04

Major Facilitator Superfamily;


Pssm-ID: 429598 [Multi-domain]  Cd Length: 344  Bit Score: 39.32  E-value: 4.38e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 392921807   59 LICFIVFFGIAFPFVESPSAALYSEILGPRKQGNMQGLFSLGGSLAPVIGSLSSTALFQASGFRYVMVYQAVVLVIGALL 138
Cdd:pfam07690  88 LLVLRVLQGLGAGALFPAALALIADWFPPEERGRALGLVSAGFGLGAALGPLLGGLLASLFGWRAAFLILAILSLLAAVL 167

                          90
                  ....*....|....*....
gi 392921807  139 IGVFYKRLVPLKLKSIKKI 157
Cdd:pfam07690 168 LLLPRPPPESKRPKPAEEA 186
 
Name Accession Description Interval E-value
MFS_MFSD8 cd17326
Major facilitator superfamily domain-containing protein 8; Major facilitator superfamily (MFS) ...
 57-147 3.01e-22

Major facilitator superfamily domain-containing protein 8; Major facilitator superfamily (MFS) domain-containing protein 8 (MFSD8) is also called ceroid-lipofuscinosis neuronal protein 7 (CLN7). It is a polytopic lysosomal membrane protein that may transport small solutes by using chemiosmotic ion gradients. Mutations in MFSD8/CLN7 cause a variant of late-infantile neuronal ceroid lipofuscinoses (vLINCL), a neurodegenerative lysosomal storage disorder. Some variants are associated with nonsyndromic autosomal recessive macular dystrophy. MFSD8/CLN7 belongs to the Eukaryotic Solute carrier 46 (SLC46)/Bacterial Tetracycline resistance -like (SLC46/TetA-like) family of the Major Facilitator Superfamily (MFS) of transporters. MFS proteins are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.


Pssm-ID: 340884 [Multi-domain]  Cd Length: 374  Bit Score: 90.73  E-value: 3.01e-22
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 392921807  57 VYLICFIVFFGIAFPFVESPSAALYSEILGPRKQGNMQGLFSLGGSLAPVIGSLSSTALFQASGFRYVMVYQAVVLVIGA 136
Cdd:cd17326  284 PWGITGVILFGLGYPIGNVMLYTLYSKILGPKPQGTMQGVLTASGSLARTLGPIFVTAIYTHFGPRWTWIITLGLLLLAI 363

                         90
                 ....*....|.
gi 392921807 137 LLIGVFYKRLV 147
Cdd:cd17326  364 ILLIVFYKRLV 374
MFS_MdtG_SLC18_like cd17325
bacterial MdtG-like and eukaryotic solute carrier 18 (SLC18) family of the Major Facilitator ...
 57-143 1.92e-05

bacterial MdtG-like and eukaryotic solute carrier 18 (SLC18) family of the Major Facilitator Superfamily of transporters; This family is composed of eukaryotic solute carrier 18 (SLC18) family transporters and related bacterial multidrug resistance (MDR) transporters including several proteins from Escherichia coli such as multidrug resistance protein MdtG, from Bacillus subtilis such as multidrug resistance proteins 1 (Bmr1) and 2 (Bmr2), and from Staphylococcus aureus such as quinolone resistance protein NorA. The family also includes Escherichia coli arabinose efflux transporters YfcJ and YhhS. MDR transporters are drug/H+ antiporters (DHA) that mediate the efflux of a variety of drugs and toxic compounds, and confer resistance to these compounds. The SLC18 transporter family includes vesicular monoamine transporters (VAT1 and VAT2), vesicular acetylcholine transporter (VAChT), and SLC18B1, which is proposed to be a vesicular polyamine transporter (VPAT). The MdtG/SLC18 family belongs to the Major Facilitator Superfamily (MFS) of membrane transport proteins, which are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.


Pssm-ID: 340883 [Multi-domain]  Cd Length: 375  Bit Score: 43.33  E-value: 1.92e-05
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 392921807  57 VYLICFIVFFGIAFPFVESPSAALYSEILGPRKQGNMQGLFSLGGSLAPVIGSLSSTALFQASGFRYVMVYQAVVLVIGA 136
Cdd:cd17325  289 WLLLLLLALLGLGLGLVFPATLALLADIVPPEGRGTAMGLFNTAFSLGMVLGPLLGGFLYDAFGFATPFLAAAALLLLAA 368


                 ....*..
gi 392921807 137 LLIGVFY 143
Cdd:cd17325  369 VLFLLLR 375
UhpC COG2271
Sugar phosphate permease [Carbohydrate transport and metabolism];
53-146 5.26e-05

Sugar phosphate permease [Carbohydrate transport and metabolism];


Pssm-ID: 441872 [Multi-domain]  Cd Length: 363  Bit Score: 41.78  E-value: 5.26e-05
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 392921807  53 VPLPVYLICFIVFFGIAFPFVESPSAALYSEILGPRKQGNMQGLFSLGGSLAPVIGSLSSTALFQASGFRYVMVYQAVVL 132
Cdd:COG2271  268 LPSPALAIALLFLAGFGLGGAFGLLWALAAELFPKKARGTASGLVNTFGFLGGALGPLLVGYLLDATGYQAAFLLLAALA 347

                         90
                 ....*....|....
gi 392921807 133 VIGALLIGVFYKRL 146
Cdd:COG2271  348 LLAALLALLLLRET 361
AraJ COG2814
Predicted arabinose efflux permease AraJ, MFS family [Carbohydrate transport and metabolism];
59-148 1.44e-04

Predicted arabinose efflux permease AraJ, MFS family [Carbohydrate transport and metabolism];


Pssm-ID: 442063 [Multi-domain]  Cd Length: 348  Bit Score: 40.73  E-value: 1.44e-04
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 392921807  59 LICFIVFFGIAFPFVESPSAALYSEILGPRKQGNMQGLFSLGGSLAPVIGSLSSTALFQASGFRYVMVYQAVVLVIGALL 138
Cdd:COG2814  101 LLAARFLQGLGAGALFPAALALIADLVPPERRGRALGLLGAGLGLGPALGPLLGGLLADLFGWRWVFLVNAVLALLALLL 180

                         90
                 ....*....|
gi 392921807 139 IGVFYKRLVP 148
Cdd:COG2814  181 LLRLLPESRP 190
MFS_1 pfam07690
Major Facilitator Superfamily;
59-157 4.38e-04

Major Facilitator Superfamily;


Pssm-ID: 429598 [Multi-domain]  Cd Length: 344  Bit Score: 39.32  E-value: 4.38e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 392921807   59 LICFIVFFGIAFPFVESPSAALYSEILGPRKQGNMQGLFSLGGSLAPVIGSLSSTALFQASGFRYVMVYQAVVLVIGALL 138
Cdd:pfam07690  88 LLVLRVLQGLGAGALFPAALALIADWFPPEERGRALGLVSAGFGLGAALGPLLGGLLASLFGWRAAFLILAILSLLAAVL 167

                          90
                  ....*....|....*....
gi 392921807  139 IGVFYKRLVPLKLKSIKKI 157
Cdd:pfam07690 168 LLLPRPPPESKRPKPAEEA 186
NarK COG2223
Nitrate/nitrite transporter NarK [Inorganic ion transport and metabolism];
53-156 8.32e-04

Nitrate/nitrite transporter NarK [Inorganic ion transport and metabolism];


Pssm-ID: 441825 [Multi-domain]  Cd Length: 392  Bit Score: 38.32  E-value: 8.32e-04
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 392921807  53 VPLPVYLICFiVFFGIAFPFVESPSAALYSEILGPRKQGNMQGLFSLGGSLAPVIGSLSSTALFQASGFRYVMVYQAVVL 132
Cdd:COG2223   91 VSYWLLLLLG-LLLGIGGGGSFAVGIALVSKWFPPDRRGLALGLAAGGGNLGAAVAAFLAPLLIAAFGWRNAFLILGILL 169

                         90       100
                 ....*....|....*....|....
gi 392921807 133 VIGALLIGVFYKRLVPLKLKSIKK 156
Cdd:COG2223  170 LVVAVLAWLFLRDPPAGAAAAAKA 193
NarK COG2223
Nitrate/nitrite transporter NarK [Inorganic ion transport and metabolism];
54-145 1.47e-03

Nitrate/nitrite transporter NarK [Inorganic ion transport and metabolism];


Pssm-ID: 441825 [Multi-domain]  Cd Length: 392  Bit Score: 37.55  E-value: 1.47e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 392921807  54 PLPVYLICFIVFFgIAFPFVESPSAALYSEILGPRKQGNMQGLFSLGGSLAPVIGSLSSTALFQASG-FRYVMVYQAVVL 132
Cdd:COG2223  294 SLWLFLVLFLLLG-LALGGGNGAVFALVPDIFPTKNAGAVYGLVGAAGGLGGFLGPLLFGALLDATGsYTAAFLVFAVLA 372

                         90
                 ....*....|...
gi 392921807 133 VIGALLIGVFYKR 145
Cdd:COG2223  373 LVALVLTLLLYRR 385
MFS_MefA_like cd06173
Macrolide efflux protein A and similar proteins of the Major Facilitator Superfamily of ...
 54-139 2.14e-03

Macrolide efflux protein A and similar proteins of the Major Facilitator Superfamily of transporters; This family is composed of Streptococcus pyogenes macrolide efflux protein A (MefA) and similar transporters, many of which remain uncharacterized. Some members may be multidrug resistance (MDR) transporters, which are drug/H+ antiporters (DHAs) that mediate the efflux of a variety of drugs and toxic compounds, conferring resistance to these compounds. MefA confers resistance to 14-membered macrolides including erythromycin and to 15-membered macrolides. It functions as an efflux pump to regulate intracellular macrolide levels. The MefA-like family belongs to the Major Facilitator Superfamily (MFS) of membrane transport proteins, which are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.


Pssm-ID: 340863 [Multi-domain]  Cd Length: 383  Bit Score: 37.21  E-value: 2.14e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 392921807  54 PLPVYLICFiVFFGIAFPFVESPSAALYSEILGPRKQGNMQGLFSLGGSLAPVIGSLSSTALFQASGFRYVMVYQAVVLV 133
Cdd:cd06173  296 SLWLLLAAL-FLLGLAGGLFNVPLNTLLQLRVPDELRGRVFSVYNALNSGAMPLGALLAGLLADALGLSAVFLLAGALLL 374


                 ....*.
gi 392921807 134 IGALLI 139
Cdd:cd06173  375 LAALLL 380
UhpC COG2271
Sugar phosphate permease [Carbohydrate transport and metabolism];
59-138 4.84e-03

Sugar phosphate permease [Carbohydrate transport and metabolism];


Pssm-ID: 441872 [Multi-domain]  Cd Length: 363  Bit Score: 36.00  E-value: 4.84e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 392921807  59 LICFIVFFGIAFPFVESPSAALYSEILGPRKQGNMQGLFSLGGSLAPVIGSLSSTALFQASGFRYVMVYQAVVLVIGALL 138
Cdd:COG2271  101 LLLLRFLLGLGEAGFFPAALKLIAEWFPPKERGRALGIFNAGGPLGGALAPPLLGWLLAAFGWRAAFLILGLPGLLLALL 180
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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