RhoGAP: GTPase-activator protein (GAP) for Rho-like GTPases; GAPs towards Rho/Rac/Cdc42-like small GTPases. Small GTPases (G proteins) cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when bound to GDP. The Rho family of small G proteins, which includes Cdc42Hs, activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. G proteins generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude. The RhoGAPs are one of the major classes of regulators of Rho G proteins.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 392901662 333 QGVIAANETLetlcskSPSQIPIYLEKCIQFIEQnGGFEQEGLYRVPGNQTHLAEVEKRFLKYGEFDVSSFDTPVHVAAT 412
Cdd:cd04373    1 FGVPLANVVT------SEKPIPIFLEKCVEFIEA-TGLETEGIYRVSGNKTHLDSLQKQFDQDHNLDLVSKDFTVNAVAG 73

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 392901662 413 ALKSFFSCLPESLIPTAYHLRWKQIMMVSDDIKKIDGIRDALAILPVSNQKVLQYLVTHLTKVSC-SPKTVMNSNNLSKV 491
Cdd:cd04373   74 ALKSFFSELPDPLIPYSMHLELVEAAKINDREQRLHALKELLKKFPPENFDVFKYVITHLNKVSQnSKVNLMTSENLSIC 153

                        170       180       190
                 ....*....|....*....|....*....|...
gi 392901662 492 WTPTLFRPVFASYEELsSGIIAFQLALEMLIFN 524
Cdd:cd04373  154 FWPTLMRPDFTSMEAL-SATRIYQTIIETFIQQ 185

RhoGAP_p190: RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain of p190-like proteins. p190, also named RhoGAP5, plays a role in neuritogenesis and axon branch stability. p190 shows a preference for Rho, over Rac and Cdc42, and consists of an N-terminal GTPase domain and a C-terminal GAP domain. The central portion of p190 contains important regulatory phosphorylation sites. Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 392901662 333 QGVIAANETLetlcskSPSQIPIYLEKCIQFIEQnGGFEQEGLYRVPGNQTHLAEVEKRFLKYGEFDVSSFDTPVHVAAT 412
Cdd:cd04373    1 FGVPLANVVT------SEKPIPIFLEKCVEFIEA-TGLETEGIYRVSGNKTHLDSLQKQFDQDHNLDLVSKDFTVNAVAG 73

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 392901662 413 ALKSFFSCLPESLIPTAYHLRWKQIMMVSDDIKKIDGIRDALAILPVSNQKVLQYLVTHLTKVSC-SPKTVMNSNNLSKV 491
Cdd:cd04373   74 ALKSFFSELPDPLIPYSMHLELVEAAKINDREQRLHALKELLKKFPPENFDVFKYVITHLNKVSQnSKVNLMTSENLSIC 153

                        170       180       190
                 ....*....|....*....|....*....|...
gi 392901662 492 WTPTLFRPVFASYEELsSGIIAFQLALEMLIFN 524
Cdd:cd04373  154 FWPTLMRPDFTSMEAL-SATRIYQTIIETFIQQ 185

RhoGAP domain; GTPase activator proteins towards Rho/Rac/Cdc42-like small GTPases.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 392901662  354 PIYLEKCIQFIEQNGgFEQEGLYRVPGNQTHLAEVEKRFLKYGEFDVSSFDTPVHVAATALKSFFSCLPESLIPTAYHLR 433
Cdd:pfam00620   1 PLIVRKCVEYLEKRG-LDTEGIFRVSGSASRIKELREAFDRGPDVDLDLEEEDVHVVASLLKLFLRELPEPLLTFELYEE 79

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 392901662  434 WKQIMMVSDDIKKIDGIRDALAILPVSNQKVLQYLVTHLTKVS-CSPKTVMNSNNLSKVWTPTLFRP 499
Cdd:pfam00620  80 FIEAAKLPDEEERLEALRELLRKLPPANRDTLRYLLAHLNRVAqNSDVNKMNAHNLAIVFGPTLLRP 146

RhoGAP_p190: RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain of p190-like proteins. p190, also named RhoGAP5, plays a role in neuritogenesis and axon branch stability. p190 shows a preference for Rho, over Rac and Cdc42, and consists of an N-terminal GTPase domain and a C-terminal GAP domain. The central portion of p190 contains important regulatory phosphorylation sites. Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 392901662 333 QGVIAANETLetlcskSPSQIPIYLEKCIQFIEQnGGFEQEGLYRVPGNQTHLAEVEKRFLKYGEFDVSSFDTPVHVAAT 412
Cdd:cd04373    1 FGVPLANVVT------SEKPIPIFLEKCVEFIEA-TGLETEGIYRVSGNKTHLDSLQKQFDQDHNLDLVSKDFTVNAVAG 73

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 392901662 413 ALKSFFSCLPESLIPTAYHLRWKQIMMVSDDIKKIDGIRDALAILPVSNQKVLQYLVTHLTKVSC-SPKTVMNSNNLSKV 491
Cdd:cd04373   74 ALKSFFSELPDPLIPYSMHLELVEAAKINDREQRLHALKELLKKFPPENFDVFKYVITHLNKVSQnSKVNLMTSENLSIC 153

                        170       180       190
                 ....*....|....*....|....*....|...
gi 392901662 492 WTPTLFRPVFASYEELsSGIIAFQLALEMLIFN 524
Cdd:cd04373  154 FWPTLMRPDFTSMEAL-SATRIYQTIIETFIQQ 185

RhoGAP domain; GTPase activator proteins towards Rho/Rac/Cdc42-like small GTPases.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 392901662  354 PIYLEKCIQFIEQNGgFEQEGLYRVPGNQTHLAEVEKRFLKYGEFDVSSFDTPVHVAATALKSFFSCLPESLIPTAYHLR 433
Cdd:pfam00620   1 PLIVRKCVEYLEKRG-LDTEGIFRVSGSASRIKELREAFDRGPDVDLDLEEEDVHVVASLLKLFLRELPEPLLTFELYEE 79

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 392901662  434 WKQIMMVSDDIKKIDGIRDALAILPVSNQKVLQYLVTHLTKVS-CSPKTVMNSNNLSKVWTPTLFRP 499
Cdd:pfam00620  80 FIEAAKLPDEEERLEALRELLRKLPPANRDTLRYLLAHLNRVAqNSDVNKMNAHNLAIVFGPTLLRP 146

RhoGAP_fRGD1: RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain of fungal RGD1-like proteins. Yeast Rgd1 is a GAP protein for Rho3 and Rho4 and plays a role in low-pH response. Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 392901662 342 LETLCSKSPSQIPIYLEKCIQFIEqNGGFEQEGLYRVPGNQTHL----AEVEKRFLKYGEFDVSSFDTPVHVAATALKSF 417
Cdd:cd04398    5 LEDLILREGDNVPNIVYQCIQAIE-NFGLNLEGIYRLSGNVSRVnklkELFDKDPLNVLLISPEDYESDIHSVASLLKLF 83

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 392901662 418 FSCLPESLIPTAYHLRWKQIMMVSDDIKKIDGIRDALAILPVSNQKVLQYLVTHLTKV-SCSPKTVMNSNNLSKVWTPTL 496
Cdd:cd04398   84 FRELPEPLLTKALSREFIEAAKIEDESRRRDALHGLINDLPDANYATLRALMFHLARIkEHESVNRMSVNNLAIIWGPTL 163

                        170       180       190
                 ....*....|....*....|....*....|...
gi 392901662 497 FRPVFASYEELSSgiiaFQLALEMLIFNSHSLF 529
Cdd:cd04398  164 MNAAPDNAADMSF----QSRVIETLLDNAYQIF 192

RhoGAP_ARAP: RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain present in ARAPs. ARAPs (also known as centaurin deltas) contain, besides the RhoGAP domain, an Arf GAP, ankyrin repeat ras-associating, and PH domains. Since their ArfGAP activity is PIP3-dependent, ARAPs are considered integration points for phosphoinositide, Arf and Rho signaling. Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 392901662 353 IPIYLEKCIQFIEQNGgFEQEGLYRVPGNQTHLAEVEKRFLKygefDVSSF-----DTPVHVAATALKSFFSCLPESLIP 427
Cdd:cd04385   15 IPVIVDKCIDFITQHG-LMSEGIYRKNGKNSSVKKLLEAFRK----DARSVqlregEYTVHDVADVLKRFLRDLPDPLLT 89

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 392901662 428 TAYHLRWKQIMMVSDDIKKIDGIRDALAILPVSNQKVLQYLVTHLTKVS-CSPKTVMNSNNLSKVWTPTLFRP 499
Cdd:cd04385   90 SELHAEWIEAAELENKDERIARYKELIRRLPPINRATLKVLIGHLYRVQkHSDENQMSVHNLALVFGPTLFQT 162

RhoGAP_MgcRacGAP: RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain present in MgcRacGAP proteins. MgcRacGAP plays an important dual role in cytokinesis: i) it is part of centralspindlin-complex, together with the mitotic kinesin MKLP1, which is critical for the structure of the central spindle by promoting microtuble bundling. ii) after phosphorylation by aurora B MgcRacGAP becomes an effective regulator of RhoA and plays an important role in the assembly of the contractile ring and the initiation of cytokinesis. MgcRacGAP-like proteins contain a N-terminal C1-like domain, and a C-terminal RhoGAP domain. Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 392901662 347 SKSPsQIPIYLEKCIQFIEQNGgFEQEGLYRVPGNQTHLAEVEKRFLK-YGEFDVSSFDtpVHVAATALKSFFSCLPESL 425
Cdd:cd04382   12 STSP-MIPALIVHCVNEIEARG-LTEEGLYRVSGSEREVKALKEKFLRgKTVPNLSKVD--IHVICGCLKDFLRSLKEPL 87

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 392901662 426 IPTAYHlrwkQIMMVSDDIKKIDGIRDAL--AI--LPVSNQKVLQYLVTHLTKVSCSPKTVMNSNNLSKVWTPTLF 497
Cdd:cd04382   88 ITFALW----KEFMEAAEILDEDNSRAALyqAIseLPQPNRDTLAFLILHLQRVAQSPECKMDINNLARVFGPTIV 159

RhoGAP_GMIP_PARG1: RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain of GMIP (Gem interacting protein) and PARG1 (PTPL1-associated RhoGAP1). GMIP plays important roles in neurite growth and axonal guidance, and interacts with Gem, a member of the RGK subfamily of the Ras small GTPase superfamily, through the N-terminal half of the protein. GMIP contains a C-terminal RhoGAP domain. GMIP inhibits RhoA function, but is inactive towards Rac1 and Cdc41. PARG1 interacts with Rap2, also a member of the Ras small GTPase superfamily whose exact function is unknown, and shows strong preference for Rho. Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 392901662 348 KSPSQIPIYLEKCIQFIEQNGgFEQEGLYRVPGNQthlAEVEK--RFLKYGEFDVSSFDTPVHVAATALKSFFSCLPESL 425
Cdd:cd04378   11 DFPDEVPFIIKKCTSEIENRA-LGVQGIYRVSGSK---ARVEKlcQAFENGKDLVELSELSPHDISSVLKLFLRQLPEPL 86

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 392901662 426 IPtaYHLrWKQIMMVSDDIKKIDG-----------------IRDALAILPVSNQKVLQYLVTHLTKVSC-SPKTVMNSNN 487
Cdd:cd04378   87 IL--FRL-YNDFIALAKEIQRDTEedkapntpievnriirkLKDLLRQLPASNYNTLQHLIAHLYRVAEqFEENKMSPNN 163

                        170
                 ....*....|..
gi 392901662 488 LSKVWTPTLFRP 499
Cdd:cd04378  164 LGIVFGPTLIRP 175

RhoGAP_FAM13A1a: RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain of FAM13A1, isoform a-like proteins. The function of FAM13A1a is unknown. Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by up several orders of magnitude.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 392901662 353 IPIYLEKCIQFIEQnGGFEQEGLYRVPGNQthlAEVEKRFLKY--GEFDVSSFDTPVHVAATALKSFFSCLPESLIPTAY 430
Cdd:cd04393   20 VPAVVRHIVEYLEQ-HGLEQEGLFRVNGNA---ETVEWLRQRLdsGEEVDLSKEADVCSAASLLRLFLQELPEGLIPASL 95

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 392901662 431 HlrwKQIMMVSDD--IKKIDG--IRDALAILPVSNQKVLQYLVTHLTKV-SCSPKTVMNSNNLSKVWTPTLF--RPVFAS 503
Cdd:cd04393   96 Q---IRLMQLYQDynGEDEFGrkLRDLLQQLPPVNYSLLKFLCHFLSNVaSQHHENRMTAENLAAVFGPDVFhvYTDVED 172

                 ....*.
gi 392901662 504 YEELSS 509
Cdd:cd04393  173 MKEQEI 178

RhoGAP_KIAA1688: GTPase-activator protein (GAP) domain for Rho-like GTPases found in KIAA1688-like proteins; KIAA1688 is a protein of unknown function that contains a RhoGAP domain and a myosin tail homology 4 (MyTH4) domain. Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 392901662 352 QIPIYLEKCIQFIEQNGGFEQEGLYRVPGNQTHLAEVEKRFLKyGEFDVSSFDTPvHVAATALKSFFSCLPESLIPTAYh 431
Cdd:cd04389   20 KLPWILTFLSEKVLALGGFQTEGIFRVPGDIDEVNELKLRVDQ-WDYPLSGLEDP-HVPASLLKLWLRELEEPLIPDAL- 96

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 392901662 432 lrWKQIMMVSDDIKKIDGIRDAlaiLPVSNQKVLQYLVtHLTKVSCSP----KTVMNSNNLSKVWTPTLFR 498
Cdd:cd04389   97 --YQQCISASEDPDKAVEIVQK---LPIINRLVLCYLI-NFLQVFAQPenvaHTKMDVSNLAMVFAPNILR 161

RhoGAP_Graf: GTPase-activator protein (GAP) domain for Rho-like GTPases found in GRAF (GTPase regulator associated with focal adhesion kinase); Graf is a multi-domain protein, containing SH3 and PH domains, that binds focal adhesion kinase and influences cytoskeletal changes mediated by Rho proteins. Graf exhibits GAP activity toward RhoA and Cdc42, but only weakly activates Rac1. Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 392901662 356 YLEKCIQFIEQNGgFEQEGLYRVPGNQTHLAEV-----EKRFLKYGEFDVSSFDTPVHVAATALKSFFSCLPESLIPTAY 430
Cdd:cd04374   31 FVRKCIEAVETRG-INEQGLYRVVGVNSKVQKLlslglDPKTSTPGDVDLDNSEWEIKTITSALKTYLRNLPEPLMTYEL 109

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 392901662 431 HlrwKQIMMV--SDDIK-KIDGIRDALAILPVSNQKVLQYLVTHLTKVSC-SPKTVMNSNNLSKVWTPTLFRPvfasYEE 506
Cdd:cd04374  110 H---NDFINAakSENLEsRVNAIHSLVHKLPEKNREMLELLIKHLTNVSDhSKKNLMTVSNLGVVFGPTLLRP----QEE 182

                        170       180
                 ....*....|....*....|.
gi 392901662 507 LSSGI--IAFQ-LALEMLIFN 524
Cdd:cd04374  183 TVAAImdIKFQnIVVEILIEN 203

RhoGAP_myosin_IX: RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain present in class IX myosins. Class IX myosins contain a characteristic head domain, a neck domain, a tail domain which contains a C6H2-zinc binding motif and a RhoGAP domain. Class IX myosins are single-headed, processive myosins that are partly cytoplasmic, and partly associated with membranes and the actin cytoskeleton. Class IX myosins are implicated in the regulation of neuronal morphogenesis and function of sensory systems, like the inner ear. There are two major isoforms, myosin IXA and IXB with several splice variants, which are both expressed in developing neurons. Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 392901662 351 SQIPIYLEKCIQFIEQNGGFeQEGLYRVPGNQTHLAEVeKRFLKYGEFDVSSFDTPVHVAATALKSFFSCLPESLIPTAY 430
Cdd:cd04377   13 RSVPLVLEKLLEHIEMHGLY-TEGIYRKSGSANKIKEL-RQGLDTDPDSVNLEDYPIHVITSVLKQWLRELPEPLMTFEL 90

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 392901662 431 HLRWKQIMMVSDDIKKIDGIRDALAILPVSNQKVLQYLVTHLTKVSCSPKT-VMNSNNLSKVWTPTLFR 498
Cdd:cd04377   91 YENFLRAMELEEKQERVRALYSVLEQLPRANLNTLERLIFHLVRVALQEEVnRMSANALAIVFAPCILR 159

RhoGAP_Bcr: RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain of Bcr (breakpoint cluster region protein)-like proteins. Bcr is a multidomain protein with a variety of enzymatic functions. It contains a RhoGAP and a Rho GEF domain, a Ser/Thr kinase domain, an N-terminal oligomerization domain, and a C-terminal PDZ binding domain, in addition to PH and C2 domains. Bcr is a negative regulator of: i) RacGTPase, via the Rho GAP domain, ii) the Ras-Raf-MEK-ERK pathway, via phosphorylation of the Ras binding protein AF-6, and iii) the Wnt signaling pathway through binding beta-catenin. Bcr can form a complex with beta-catenin and Tcf1. The Wnt signaling pathway is involved in cell proliferation, differentiation, and cell renewal. Bcr was discovered as a fusion partner of Abl. The Bcr-Abl fusion is characteristic for a large majority of chronic myelogenous leukemias (CML). Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 392901662 351 SQIPIYLEKCIQFIEQNGgFEQEGLYRVPGNQTHLAEVEKRF-LKYGEFDVSSFDTPVHVAATALKSFFSCLPESLIPTA 429
Cdd:cd04387   14 SKVPYIVRQCVEEVERRG-MEEVGIYRISGVATDIQALKAAFdTNNKDVSVMLSEMDVNAIAGTLKLYFRELPEPLFTDE 92

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 392901662 430 YHLRWKQIMMVSDDIKKIDGIRDALAILPVSNQKVLQYLVTHLTKVS-CSPKTVMNSNNLSKVWTPTLFRP 499
Cdd:cd04387   93 LYPNFAEGIALSDPVAKESCMLNLLLSLPDPNLVTFLFLLHHLKRVAeREEVNKMSLHNLATVFGPTLLRP 163

RhoGap_RalBP1: RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain present in RalBP1 proteins, also known as RLIP, RLIP76 or cytocentrin. RalBP1 plays an important role in endocytosis during interphase. During mitosis, RalBP1 transiently associates with the centromere and has been shown to play an essential role in the proper assembly of the mitotic apparatus. RalBP1 is an effector of the Ral GTPase which itself is an effector of Ras. RalBP1 contains a RhoGAP domain, which shows weak activity towards Rac1 and Cdc42, but not towards Ral, and a Ral effector domain binding motif. Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 392901662 352 QIPIYLEKCIQFIEQNGgFEQEGLYRVPGNQTHLAEVEKRFLKYGEFDVSSFDTPvhVAATALKSFFSCLPESLIPTAYH 431
Cdd:cd04381   19 DLPLVFRECIDYVEKHG-MKCEGIYKVSGIKSKVDELKAAYNRRESPNLEEYEPP--TVASLLKQYLRELPEPLLTKELM 95

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 392901662 432 LRWKQIMMVSDDIKKIDGIRDALAILPVSNQKVLQYLVTHLTKVSCSPK-TVMNSNNLSKVWTPTL 496
Cdd:cd04381   96 PRFEEACGRPTEAEREQELQRLLKELPECNRLLLAWLIVHMDHVIAQELeTKMNIQNISIVLSPTV 161

RhoGAP_PARG1: RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain of PARG1 (PTPL1-associated RhoGAP1). PARG1 was originally cloned as an interaction partner of PTPL1, an intracellular protein-tyrosine phosphatase. PARG1 interacts with Rap2, also a member of the Ras small GTPase superfamily whose exact function is unknown, and shows strong preference for Rho. Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 392901662 348 KSPSQIPIYLEKCIQFIEqNGGFEQEGLYRVPGNQThlaEVEK--RFLKYGEFDVSSFDTPVHVAATALKSFFSCLPESL 425
Cdd:cd04409   11 KSPDGIPFIIKKCTSEIE-SRALCLKGIYRVNGAKS---RVEKlcQAFENGKDLVELSELSPHDISNVLKLYLRQLPEPL 86

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 392901662 426 IPTAYH------LRWKQIMMVSDDIKKIDG----------------IRDALAILPVSNQKVLQYLVTHLTKVS-CSPKTV 482
Cdd:cd04409   87 ILFRLYnefiglAKESQHVNETQEAKKNSDkkwpnmctelnrillkSKDLLRQLPAPNYNTLQFLIVHLHRVSeQAEENK 166

                        170
                 ....*....|....*..
gi 392901662 483 MNSNNLSKVWTPTLFRP 499
Cdd:cd04409  167 MSASNLGIIFGPTLIRP 183

RhoGAP_ARHGAP20: RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain of ArhGAP20-like proteins. ArhGAP20, also known as KIAA1391 and RA-RhoGAP, contains a RhoGAP, a RA, and a PH domain, and ANXL repeats. ArhGAP20 is activated by Rap1 and induces inactivation of Rho, which in turn leads to neurite outgrowth. Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 392901662 369 GFEQEGLYRVPGNQTHLAEVEKRFLKYGEFDVSSfdTPVHVAATALKSFFSCLPESLIPTAYHLRWKQIMMVSDDIKKID 448
Cdd:cd04402   30 GPSTEGIFRRSANAKACKELKEKLNSGVEVDLKA--EPVLLLASVLKDFLRNIPGSLLSSDLYEEWMSALDQENEEEKIA 107

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 392901662 449 GIRDALAILPVSNQKVLQYLVTHLTKVSCSPKT-VMNSNNLSKVWTPTLFRPVfASYEELSSGIIAFQLALEMLIFNSHS 527
Cdd:cd04402  108 ELQRLLDKLPRPNVLLLKHLICVLHNISQNSETnKMDAFNLAVCIAPSLLWPP-ASSELQNEDLKKVTSLVQFLIENCQE 186

                 ..
gi 392901662 528 LF 529
Cdd:cd04402  187 IF 188

RhoGAP_ARHGAP18: RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain of ArhGAP18-like proteins. The function of ArhGAP18 is unknown. Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 392901662 348 KSP-SQIPIYLEKCIQFIEQnGGFEQEGLYRVPGNQTHLA----EVEKRFLKyGEFDvssFDT-PVHVAATALKSFFSCL 421
Cdd:cd04391   16 KVPgSKVPLIFQKLINKLEE-RGLETEGILRIPGSAQRVKflcqELEAKFYE-GTFL---WDQvKQHDAASLLKLFIREL 90

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 392901662 422 PESLIPTAYHLRWKQIMMVSDDIKKIDGIRDALAILPVSNQKVLQYLVTHLTKV-SCSPKTVMNSNNLSKVWTPTLFRP- 499
Cdd:cd04391   91 PQPLLTVEYLPAFYSVQGLPSKKDQLQALNLLVLLLPEANRDTLKALLEFLQKVvDHEEKNKMNLWNVAMIMAPNLFPPr 170

                        170       180
                 ....*....|....*....|....*....
gi 392901662 500 ------VFASYEELSSGIIAFQLALEMLI 522
Cdd:cd04391  171 gkhskdNESLQEEVNMAAGCANIMRLLIR 199

RhoGAP_SYD1: RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain present in SYD-1_like proteins. Syd-1, first identified and best studied in C.elegans, has been shown to play an important role in neuronal development by specifying axonal properties. Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 392901662 342 LETLCSKSPS--QIPIYLEKCIQFIEQNgGFEQEGLYRVPGNqthlaeVEKRFLKYGEFDVSSFDTP--------VHVAA 411
Cdd:cd04379    5 LSRLVEREGEsrDVPIVLQKCVQEIERR-GLDVIGLYRLCGS------AAKKKELRDAFERNSAAVElseelypdINVIT 77

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 392901662 412 TALKSFFSCLPESLIPTAYHLRWKQIMMVSDDIKKIDGIRDALAI---LPVSNQKVLQYLVTHLTKVSC-SPKTVMNSNN 487
Cdd:cd04379   78 GVLKDYLRELPEPLITPQLYEMVLEALAVALPNDVQTNTHLTLSIidcLPLSAKATLLLLLDHLSLVLSnSERNKMTPQN 157

                        170
                 ....*....|..
gi 392901662 488 LSKVWTPTLFRP 499
Cdd:cd04379  158 LAVCFGPVLMFC 169

RhoGAP_DLC1: RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain of DLC1-like proteins. DLC1 shows in vitro GAP activity towards RhoA and CDC42. Beside its C-terminal GAP domain, DLC1 also contains a SAM (sterile alpha motif) and a START (StAR-related lipid transfer action) domain. DLC1 has tumor suppressor activity in cell culture. Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 392901662 353 IPIYLEKCIQFIEQNGgFEQEGLYRVPGNQTHLAEVekRFLKYGEFDVSSFD-TPVHVAATALKSFFSCLPESLIPTAYH 431
Cdd:cd04375   20 LPRSIQQAMRWLRNNA-LDQVGLFRKSGVKSRIQKL--RSMIESSTDNVNYDgQQAYDVADMLKQYFRDLPEPLLTNKLS 96

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 392901662 432 LRWKQIMMVSDDIKKIDGIRDALAILPVSNQKVLQYLVTHLTKV-SCSPKTVMNSNNLSKVWTPTLF 497
Cdd:cd04375   97 ETFIAIFQYVPKEQRLEAVQCAILLLPDENREVLQTLLYFLSDVaANSQENQMTATNLAVCLAPSLF 163

RhoGAP_myosin_IXA: RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain present in myosins IXA. Class IX myosins contain a characteristic head domain, a neck domain and a tail domain which contains a C6H2-zinc binding motif and a Rho-GAP domain. Class IX myosins are single-headed, processive myosins that are partly cytoplasmic, and partly associated with membranes and the actin cytoskeleton. Class IX myosins are implicated in the regulation of neuronal morphogenesis and function of sensory systems, like the inner ear. There are two major isoforms, myosin IXA and IXB with several splice variants, which are both expressed in developing neurons. Small GTPases cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when GDP-bound. The Rho family of GTPases activates effectors involved in a wide variety of developmental processes, including regulation of cytoskeleton formation, cell proliferation and the JNK signaling pathway. GTPases generally have a low intrinsic GTPase hydrolytic activity but there are family-specific groups of GAPs that enhance the rate of GTP hydrolysis by several orders of magnitude.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 392901662 353 IPIYLEKCIQFIEQNGGFeQEGLYRVPGNQTHLAEVeKRFLKYGEFDVSSFDTPVHVAATALKSFFSCLPESLIPTAYHL 432
Cdd:cd04406   15 VPLVVEKLINYIEMHGLY-TEGIYRKSGSTNKIKEL-RQGLDTDANSVNLDDYNIHVIASVFKQWLRDLPNPLMTFELYE 92

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 392901662 433 RWKQIMMVSDDIKKIDGIRDALAILPVSNQKVLQYLVTHLTKVSCSPKT-VMNSNNLSKVWTPTLFR 498
Cdd:cd04406   93 EFLRAMGLQERRETVRGVYSVIDQLSRTHLNTLERLIFHLVRIALQEETnRMSANALAIVFAPCILR 159