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Conserved domains on  [gi|383872507|ref|NP_001244325|]
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ubiquitin-conjugating enzyme E2 variant 1 isoform f [Homo sapiens]

Protein Classification

ubiquitin-conjugating enzyme family protein( domain architecture ID 439)

ubiquitin-conjugating enzyme family protein similar to ubiquitin-conjugating enzyme E2 that catalyzes the covalent attachment of ubiquitin to other proteins

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
UBCc_UEV super family cl49610
Ubiquitin-conjugating enzyme E2, catalytic (UBCc) domain/ubiquitin E2 variant (UEV) domain; ...
 9-100 1.66e-60

Ubiquitin-conjugating enzyme E2, catalytic (UBCc) domain/ubiquitin E2 variant (UEV) domain; The family includes ubiquitin-conjugating enzyme E2, catalytic (UBCc) domain and ubiquitin (Ub) E2 variant (UEV) domain. They belong to the ubiquitin-conjugating (UBC) superfamily that represents a structural domain with an alpha-beta(4)-alpha(3) core fold. E2 is part of the ubiquitin-mediated protein degradation pathway in which a thioester linkage forms between a conserved cysteine and the C-terminus of ubiquitin, and complexes with ubiquitin protein ligase enzymes, E3. This pathway regulates many fundamental cellular processes. There are also other E2s which form thioester linkages without the use of E3s. Several UBC homologs (TSG101, Mms2, Croc-1 and similar proteins) contains the UEV domain, which lacks the active site cysteine essential for ubiquitination and appear to function in DNA repair pathways.


The actual alignment was detected with superfamily member cd23807:

Pssm-ID: 483950  Cd Length: 134  Bit Score: 181.15  E-value: 1.66e-60
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 383872507   9 VKVPRNFRLLEELEEGQKGVGDGTVSWGLEDDEDMTLTRWTGMIIGPPR------------------------------- 57
Cdd:cd23807    1 VVVPRNFRLLEELEKGEKGVGDGTVSWGLEDDDDMTLTHWNGTIIGPPGtvfenriyslkiecgenypdepptvrfvtki 80

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|....
gi 383872507  58 -----------VDPRAISVLAKWQNSYSIKVVLQELRRLMMSKENMKLPQPPEG 100
Cdd:cd23807   81 nlpcvdqsngvVDPRKFPVLKNWQRNYTIETVLTELRREMTSKENRKLPQPPEG 134
 
Name Accession Description Interval E-value
UEV_UBE2V cd23807
ubiquitin E2 variant (UEV) domain of ubiquitin-conjugating enzyme E2 variant 1, 2 and related ...
 9-100 1.66e-60

ubiquitin E2 variant (UEV) domain of ubiquitin-conjugating enzyme E2 variant 1, 2 and related proteins; The E2V subfamily includes mammalian ubiquitin-conjugating enzyme E2 variant 1 (UBE2V1/CROC1/UBE2V/UEV1) and variant 2 (UBE2V2/MMS2/UEV2), yeast ubiquitin-conjugating enzyme variant MMS2, plant ubiquitin-conjugating enzyme E2 variant 1A (UEV1A/MMZ1) and variant 1B (UEV1B/MMZ2), and similar proteins. They have no ubiquitin ligase activity on their own. UBE2V1 (also called UEV-1, CROC-1, or TRAF6-regulated IKK activator 1 beta Uev1A) and UBE2V2 (also called DDVit 1, enterocyte differentiation-associated factor 1 (EDAF-1), enterocyte differentiation-promoting factor 1 (EDPF-1), MMS2 homolog, or vitamin D3-inducible protein) form heterodimers with UBE2N, respectively. Saccharomyces cerevisiae UEV MMS2 has a role in the DNA error-free postreplication repair (PRR) pathway. It forms a heterodimer with UBC13. Arabidopsis thaliana UEV1A and UEV1A form heterodimers with UBC35 or UBC36. The heterodimers possess ubiquitin-conjugating enzyme (EC 2.3.2.23) activity, catalyzing the synthesis of non-canonical poly-ubiquitin chains that are linked through 'Lys-63'. This type of poly-ubiquitination does not lead to protein degradation by the proteasome. Members of this subfamily may mediate transcriptional activation of target genes and plays a role in the control of progress through the cell cycle and differentiation. UEVs are homologous to E2 ubiquitin ligases but lack the conserved cysteine residue required for catalytic activity.


Pssm-ID: 467427  Cd Length: 134  Bit Score: 181.15  E-value: 1.66e-60
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 383872507   9 VKVPRNFRLLEELEEGQKGVGDGTVSWGLEDDEDMTLTRWTGMIIGPPR------------------------------- 57
Cdd:cd23807    1 VVVPRNFRLLEELEKGEKGVGDGTVSWGLEDDDDMTLTHWNGTIIGPPGtvfenriyslkiecgenypdepptvrfvtki 80

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|....
gi 383872507  58 -----------VDPRAISVLAKWQNSYSIKVVLQELRRLMMSKENMKLPQPPEG 100
Cdd:cd23807   81 nlpcvdqsngvVDPRKFPVLKNWQRNYTIETVLTELRREMTSKENRKLPQPPEG 134
UBCc smart00212
Ubiquitin-conjugating enzyme E2, catalytic domain homologues; Proteins destined for ...
 16-100 9.84e-12

Ubiquitin-conjugating enzyme E2, catalytic domain homologues; Proteins destined for proteasome-mediated degradation may be ubiquitinated. Ubiquitination follows conjugation of ubiquitin to a conserved cysteine residue of UBC homologues. This pathway functions in regulating many fundamental processes required for cell viability.TSG101 is one of several UBC homologues that lacks this active site cysteine.


Pssm-ID: 214562 [Multi-domain]  Cd Length: 145  Bit Score: 57.30  E-value: 9.84e-12
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 383872507    16 RLLEELEEGQKGVGDGTVSWGLEDDedmTLTRWTGMIIGP-----------------------------------PRVDP 60
Cdd:smart00212   1 RLLKELKELRKDPPPGFTAYPVDDE---NLLEWTGTIVGPpgtpyeggvfkltiefpedypfkppkvkfitkiyhPNVDS 77

                           90       100       110       120
                   ....*....|....*....|....*....|....*....|....*
gi 383872507    61 R---AISVL--AKWQNSYSIKVVLQELRRlMMSKENMKLPQPPEG 100
Cdd:smart00212  78 SgeiCLDILkqEKWSPALTLETVLLSLQS-LLSEPNPDSPLNADA 121
 
Name Accession Description Interval E-value
UEV_UBE2V cd23807
ubiquitin E2 variant (UEV) domain of ubiquitin-conjugating enzyme E2 variant 1, 2 and related ...
 9-100 1.66e-60

ubiquitin E2 variant (UEV) domain of ubiquitin-conjugating enzyme E2 variant 1, 2 and related proteins; The E2V subfamily includes mammalian ubiquitin-conjugating enzyme E2 variant 1 (UBE2V1/CROC1/UBE2V/UEV1) and variant 2 (UBE2V2/MMS2/UEV2), yeast ubiquitin-conjugating enzyme variant MMS2, plant ubiquitin-conjugating enzyme E2 variant 1A (UEV1A/MMZ1) and variant 1B (UEV1B/MMZ2), and similar proteins. They have no ubiquitin ligase activity on their own. UBE2V1 (also called UEV-1, CROC-1, or TRAF6-regulated IKK activator 1 beta Uev1A) and UBE2V2 (also called DDVit 1, enterocyte differentiation-associated factor 1 (EDAF-1), enterocyte differentiation-promoting factor 1 (EDPF-1), MMS2 homolog, or vitamin D3-inducible protein) form heterodimers with UBE2N, respectively. Saccharomyces cerevisiae UEV MMS2 has a role in the DNA error-free postreplication repair (PRR) pathway. It forms a heterodimer with UBC13. Arabidopsis thaliana UEV1A and UEV1A form heterodimers with UBC35 or UBC36. The heterodimers possess ubiquitin-conjugating enzyme (EC 2.3.2.23) activity, catalyzing the synthesis of non-canonical poly-ubiquitin chains that are linked through 'Lys-63'. This type of poly-ubiquitination does not lead to protein degradation by the proteasome. Members of this subfamily may mediate transcriptional activation of target genes and plays a role in the control of progress through the cell cycle and differentiation. UEVs are homologous to E2 ubiquitin ligases but lack the conserved cysteine residue required for catalytic activity.


Pssm-ID: 467427  Cd Length: 134  Bit Score: 181.15  E-value: 1.66e-60
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 383872507   9 VKVPRNFRLLEELEEGQKGVGDGTVSWGLEDDEDMTLTRWTGMIIGPPR------------------------------- 57
Cdd:cd23807    1 VVVPRNFRLLEELEKGEKGVGDGTVSWGLEDDDDMTLTHWNGTIIGPPGtvfenriyslkiecgenypdepptvrfvtki 80

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|....
gi 383872507  58 -----------VDPRAISVLAKWQNSYSIKVVLQELRRLMMSKENMKLPQPPEG 100
Cdd:cd23807   81 nlpcvdqsngvVDPRKFPVLKNWQRNYTIETVLTELRREMTSKENRKLPQPPEG 134
UBCc smart00212
Ubiquitin-conjugating enzyme E2, catalytic domain homologues; Proteins destined for ...
 16-100 9.84e-12

Ubiquitin-conjugating enzyme E2, catalytic domain homologues; Proteins destined for proteasome-mediated degradation may be ubiquitinated. Ubiquitination follows conjugation of ubiquitin to a conserved cysteine residue of UBC homologues. This pathway functions in regulating many fundamental processes required for cell viability.TSG101 is one of several UBC homologues that lacks this active site cysteine.


Pssm-ID: 214562 [Multi-domain]  Cd Length: 145  Bit Score: 57.30  E-value: 9.84e-12
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 383872507    16 RLLEELEEGQKGVGDGTVSWGLEDDedmTLTRWTGMIIGP-----------------------------------PRVDP 60
Cdd:smart00212   1 RLLKELKELRKDPPPGFTAYPVDDE---NLLEWTGTIVGPpgtpyeggvfkltiefpedypfkppkvkfitkiyhPNVDS 77

                           90       100       110       120
                   ....*....|....*....|....*....|....*....|....*
gi 383872507    61 R---AISVL--AKWQNSYSIKVVLQELRRlMMSKENMKLPQPPEG 100
Cdd:smart00212  78 SgeiCLDILkqEKWSPALTLETVLLSLQS-LLSEPNPDSPLNADA 121
UBCc_ScPEX4-like cd23812
Ubiquitin-conjugating enzyme E2, catalytic (UBCc) domain of Saccharomyces cerevisiae Peroxin-4 ...
 16-56 2.62e-03

Ubiquitin-conjugating enzyme E2, catalytic (UBCc) domain of Saccharomyces cerevisiae Peroxin-4 (PEX4) protein and related proteins; Saccharomyces cerevisiae PEX4 (EC 2.3.2.23), also called ubiquitin-conjugating enzyme E2-21 kDa, UBC10, or PAS2, acts as a ubiquitin-conjugating enzyme that catalyzes the covalent attachment of ubiquitin to other proteins. It is essential for peroxisome biogenesis and is required for UBC4-independent ubiquitination of PEX5. This subfamily also includes Arabidopsis thaliana PEX4 (also known as UBC21, EC 2.3.2.23) that is required for peroxisome biogenesis. It is necessary for the developmental elimination of obsolete peroxisome matrix proteins. It may be involved in the ubiquitination of PEX5, targeting it for recycling.


Pssm-ID: 467432 [Multi-domain]  Cd Length: 145  Bit Score: 34.83  E-value: 2.62e-03
                         10        20        30        40
                 ....*....|....*....|....*....|....*....|.
gi 383872507  16 RLLEELEEGQKGVGDGTVSWGLEDDEDmtLTRWTGMIIGPP 56
Cdd:cd23812    2 RLLKELRELQKEPNDPDIVLGPVEDDD--LFRWEAVIKGPK 40
UBCc_UBE2G1 cd23795
Ubiquitin-conjugating enzyme E2, catalytic (UBCc) domain of ubiquitin-conjugating enzyme E2 G1 ...
 17-56 4.35e-03

Ubiquitin-conjugating enzyme E2, catalytic (UBCc) domain of ubiquitin-conjugating enzyme E2 G1 and related proteins; The subfamily includes mammalian ubiquitin-conjugating enzymes E2 G1 (UBE2G1/UBC7/E217K), fission yeast ubiquitin-conjugating enzyme E2 15 (UBC15), plant ubiquitin-conjugating enzymes E2 7 (UBC7), E2 13 (UBC13) and E2 14 (UBC14). They are ubiquitin-conjugating enzymes (EC 2.3.2.23) that accept ubiquitin from the E1 complex and catalyze the covalent attachment to other proteins. In vitro, UBE2G1 catalyzes 'Lys-48'-, as well as 'Lys-63'-linked polyubiquitination. It may be involved in the degradation of muscle-specific proteins and may mediate polyubiquitination of CYP3A4. Schizosaccharomyces pombe UBC15 has a role in the formation of chromatin structures that influence the localization of transcriptional silencing factors. Arabidopsis thaliana UBC7, UBC13 and UBC14 are involved in the formation of multiubiquitin chains. They signal the protein for selective degradation.


Pssm-ID: 467415  Cd Length: 155  Bit Score: 34.45  E-value: 4.35e-03
                         10        20        30        40
                 ....*....|....*....|....*....|....*....|
gi 383872507  17 LLEELEEGQKGVGDGtVSWGLEDDEDmtLTRWTGMIIGPP 56
Cdd:cd23795    6 LRKQLKELQKNPVEG-FSAGLVDDSN--IYEWEVMIIGPP 42
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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