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Conserved domains on  [gi|334848158|ref|NP_001229353|]
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inactive ubiquitin thioesterase OTULINL isoform 2 [Mus musculus]

Protein Classification

OTU domain-containing protein( domain architecture ID 1904167)

OTU (ovarian tumor) domain-containing protein may function as a deubiquitinase (DUBs)/ubiquitin thiolesterase that catalyzes the thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin, or may be inactive

EC:  3.4.19.12
PubMed:  10664582|23827681

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
OTU super family cl45892
OTU (ovarian tumor) domain family; The OTU family of cysteine proteases use a conserved ...
 92-296 1.81e-146

OTU (ovarian tumor) domain family; The OTU family of cysteine proteases use a conserved cysteine and histidine, and in most cases an aspartate, as the catalytic triad. OTU domains typically function as deubiquitinases (DUBs)/ubiquitin thiolesterases (EC 3.4.19.12) that catalyze the thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin, a small regulatory protein that can be conjugated to a large range of target proteins. Protein ubiquitination is a post-translational modification of mostly Lys residues that regulates many cellular processes, including protein degradation, intracellular trafficking, cell signaling, autophagy, transcription, translation, and the DNA damage response. These DUBs may play important regulatory roles at the level of protein turnover by preventing degradation.


The actual alignment was detected with superfamily member cd22798:

Pssm-ID: 459237  Cd Length: 261  Bit Score: 412.66  E-value: 1.81e-146
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 334848158  92 DLLSYCAREWKGEAPRARLMRKAYEELFWRHHIKCVRAVKRDNYDALRSVLFQIFSQGLSFPSWMKEKDIVKLPEKLLFS 171
Cdd:cd22798    1 DLLEYCAREWKGETPRAKQMRKAYEELFWRHHIKYVRQVRGDNYCALRAVLFQIFSQGIPFPSWMKEQDILKLPEKLLYS 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 334848158 172 QGCNWIQQYSFGPEKYTGSNVFGKLRKCVELLKLQWTEFSGMRDHHKRGSMCNSLFSDAILECKLYEALKFLMLYQVTEA 251
Cdd:cd22798   81 QGCNWIQQYSFGPEKYTGPNVFGKLRKCVETLKTQWTEISGIKDYEKRGKMCNTLFSDEAKEYKLYEAIKFLMLYQVIEV 160

                        170       180       190       200
                 ....*....|....*....|....*....|....*....|....*
gi 334848158 252 YEQMKTNKVIPSLFRLLFSRESSPDPLSFMMNHLNSIGDTCGLDQ 296
Cdd:cd22798  161 YEQMKTGQDVPNFFSLLFSRDTSSDPLSFMMNHLNSIGDTGGLEQ 205
 
Name Accession Description Interval E-value
OTU_OTULL cd22798
OTU (ovarian tumor) domain of inactive ubiquitin thioesterase Otulinl; Otulinl, also called ...
 92-296 1.81e-146

OTU (ovarian tumor) domain of inactive ubiquitin thioesterase Otulinl; Otulinl, also called FAM105A, is an OTU-class pseudo-deubiquitinase with a disrupted catalytic triad and undetectable cleavage activity for any diubiquitin linkage. It may play a role in endoplasmic reticulum (ER)-organelle communication. Otulinl belongs to the OTU family of cysteine proteases that use a conserved cysteine and histidine, and in some cases an aspartate, as the catalytic dyad (or triad).


Pssm-ID: 438619  Cd Length: 261  Bit Score: 412.66  E-value: 1.81e-146
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 334848158  92 DLLSYCAREWKGEAPRARLMRKAYEELFWRHHIKCVRAVKRDNYDALRSVLFQIFSQGLSFPSWMKEKDIVKLPEKLLFS 171
Cdd:cd22798    1 DLLEYCAREWKGETPRAKQMRKAYEELFWRHHIKYVRQVRGDNYCALRAVLFQIFSQGIPFPSWMKEQDILKLPEKLLYS 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 334848158 172 QGCNWIQQYSFGPEKYTGSNVFGKLRKCVELLKLQWTEFSGMRDHHKRGSMCNSLFSDAILECKLYEALKFLMLYQVTEA 251
Cdd:cd22798   81 QGCNWIQQYSFGPEKYTGPNVFGKLRKCVETLKTQWTEISGIKDYEKRGKMCNTLFSDEAKEYKLYEAIKFLMLYQVIEV 160

                        170       180       190       200
                 ....*....|....*....|....*....|....*....|....*
gi 334848158 252 YEQMKTNKVIPSLFRLLFSRESSPDPLSFMMNHLNSIGDTCGLDQ 296
Cdd:cd22798  161 YEQMKTGQDVPNFFSLLFSRDTSSDPLSFMMNHLNSIGDTGGLEQ 205
Peptidase_C101 pfam16218
Peptidase family C101; This is a family of cysteine-peptidases that is conserved in ...
 85-296 5.64e-102

Peptidase family C101; This is a family of cysteine-peptidases that is conserved in vertebrates. The key residues as found in SwissProt:Q96BN8 are Asp126, Cys129, His339 and Asn341.


Pssm-ID: 465075  Cd Length: 265  Bit Score: 300.00  E-value: 5.64e-102
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 334848158   85 LSVEAEVDLLSYCAREWKGEAPRARLMRKAYEELFwrHHIKCVRAVKRDNYDALRSVLFQIFSQGLSFPSWMKEKDIVKL 164
Cdd:pfam16218   1 LSVAPEVDILDYSEREWRGNTAKAALMRKGYEEVS--QKFSSLRRVRGDNYCALRATLFQILSQSTQLPSWLQDEDILML 78

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 334848158  165 PEKLLFSQgcNWIQQYSFGPE-KYTGSNVFGKLRKCVELLKLQWTEFSGMRDHHKRGSMCNSLFSDAILECKLYEALKFL 243
Cdd:pfam16218  79 PEKLQTKY--NWIKQWTFPPEcPYGGKNAVEKLKECLELLKTKWQEAVECKTHEERQSACDELFSGEEEEYKLYEALKFL 156

                         170       180       190       200       210
                  ....*....|....*....|....*....|....*....|....*....|...
gi 334848158  244 MLYQVTEAYEQMKTNKVIPSLFRLLFSRESSPDPLSFMMNHLNSIGDTCGLDQ 296
Cdd:pfam16218 157 MLNTAIELYEDMEKGKEVPVFCWLLFARDTSSDPESFLMNHLNQVGDSGGLEQ 209
 
Name Accession Description Interval E-value
OTU_OTULL cd22798
OTU (ovarian tumor) domain of inactive ubiquitin thioesterase Otulinl; Otulinl, also called ...
 92-296 1.81e-146

OTU (ovarian tumor) domain of inactive ubiquitin thioesterase Otulinl; Otulinl, also called FAM105A, is an OTU-class pseudo-deubiquitinase with a disrupted catalytic triad and undetectable cleavage activity for any diubiquitin linkage. It may play a role in endoplasmic reticulum (ER)-organelle communication. Otulinl belongs to the OTU family of cysteine proteases that use a conserved cysteine and histidine, and in some cases an aspartate, as the catalytic dyad (or triad).


Pssm-ID: 438619  Cd Length: 261  Bit Score: 412.66  E-value: 1.81e-146
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 334848158  92 DLLSYCAREWKGEAPRARLMRKAYEELFWRHHIKCVRAVKRDNYDALRSVLFQIFSQGLSFPSWMKEKDIVKLPEKLLFS 171
Cdd:cd22798    1 DLLEYCAREWKGETPRAKQMRKAYEELFWRHHIKYVRQVRGDNYCALRAVLFQIFSQGIPFPSWMKEQDILKLPEKLLYS 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 334848158 172 QGCNWIQQYSFGPEKYTGSNVFGKLRKCVELLKLQWTEFSGMRDHHKRGSMCNSLFSDAILECKLYEALKFLMLYQVTEA 251
Cdd:cd22798   81 QGCNWIQQYSFGPEKYTGPNVFGKLRKCVETLKTQWTEISGIKDYEKRGKMCNTLFSDEAKEYKLYEAIKFLMLYQVIEV 160

                        170       180       190       200
                 ....*....|....*....|....*....|....*....|....*
gi 334848158 252 YEQMKTNKVIPSLFRLLFSRESSPDPLSFMMNHLNSIGDTCGLDQ 296
Cdd:cd22798  161 YEQMKTGQDVPNFFSLLFSRDTSSDPLSFMMNHLNSIGDTGGLEQ 205
Peptidase_C101 pfam16218
Peptidase family C101; This is a family of cysteine-peptidases that is conserved in ...
 85-296 5.64e-102

Peptidase family C101; This is a family of cysteine-peptidases that is conserved in vertebrates. The key residues as found in SwissProt:Q96BN8 are Asp126, Cys129, His339 and Asn341.


Pssm-ID: 465075  Cd Length: 265  Bit Score: 300.00  E-value: 5.64e-102
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 334848158   85 LSVEAEVDLLSYCAREWKGEAPRARLMRKAYEELFwrHHIKCVRAVKRDNYDALRSVLFQIFSQGLSFPSWMKEKDIVKL 164
Cdd:pfam16218   1 LSVAPEVDILDYSEREWRGNTAKAALMRKGYEEVS--QKFSSLRRVRGDNYCALRATLFQILSQSTQLPSWLQDEDILML 78

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 334848158  165 PEKLLFSQgcNWIQQYSFGPE-KYTGSNVFGKLRKCVELLKLQWTEFSGMRDHHKRGSMCNSLFSDAILECKLYEALKFL 243
Cdd:pfam16218  79 PEKLQTKY--NWIKQWTFPPEcPYGGKNAVEKLKECLELLKTKWQEAVECKTHEERQSACDELFSGEEEEYKLYEALKFL 156

                         170       180       190       200       210
                  ....*....|....*....|....*....|....*....|....*....|...
gi 334848158  244 MLYQVTEAYEQMKTNKVIPSLFRLLFSRESSPDPLSFMMNHLNSIGDTCGLDQ 296
Cdd:pfam16218 157 MLNTAIELYEDMEKGKEVPVFCWLLFARDTSSDPESFLMNHLNQVGDSGGLEQ 209
OTU_OTUL-like cd22790
OTU (ovarian tumor) domain of ubiquitin thioesterase otulin family; Otulin family includes ...
 95-296 4.47e-87

OTU (ovarian tumor) domain of ubiquitin thioesterase otulin family; Otulin family includes otulin and otulinl. Otulin, also called FAM105B, deubiquitinating enzyme otulin, OTU domain-containing deubiquitinase with linear linkage specificity, or ubiquitin thioesterase Gumby, is a deubiquitinase (DUB)/ubiquitin thioesterase (EC 3.4.19.12) that specifically removes linear ('Met-1'-linked) polyubiquitin chains to substrates and acts as a regulator of angiogenesis and innate immune response. It acts as a key negative regulator of inflammation by restricting spontaneous inflammation and maintaining immune homeostasis. Otulinl, also called FAM105A, is an OTU-class pseudo-deubiquitinase with a disrupted catalytic triad and undetectable cleavage activity for any diubiquitin linkage. It may play a role in endoplasmic reticulum (ER)-organelle communication. Otulin and otulinl belong to the OTU family of cysteine proteases that use a conserved cysteine and histidine, and in some cases an aspartate, as the catalytic dyad (or triad).


Pssm-ID: 438611  Cd Length: 258  Bit Score: 261.78  E-value: 4.47e-87
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 334848158  95 SYCAREWKGEAPRARLMRKAYEELFWRHHIKCVRAVKRDNYDALRSVLFQIFSQGLSFPSWmkEKDIVKLPEKLLFSQGC 174
Cdd:cd22790    1 DYAEREWKGETPKAKTIKKGYEEIPRLLGCKYLRRIRGDNYCAIRAALFQVLSQGIPVPSK--WPALEQIPEKLLNSYGC 78

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 334848158 175 NWIQQYSFGPE-KYTGSNVFGKLRKCVELLKLQWTEFSGMRDHHKRGSMCNSLF-SDAILECKLYEALKFLMLYQVTEAY 252
Cdd:cd22790   79 SWLQQWSFANRlPYTGEDVLSGLRECLLTLDSQVEELESMSTEEDREDALLSLLnSDPTLDLKLMEAVKLLMLVSAIELY 158

                        170       180       190       200
                 ....*....|....*....|....*....|....*....|....
gi 334848158 253 EQMKTNKVIPSLFRLLFSRESSPDPLSFMMNHLNSIGDTCGLDQ 296
Cdd:cd22790  159 NRMQKGEDVPLFAWLLFARDTSSTPKDFLKNHLNPVGDTAGLEQ 202
OTU_OTUL cd22799
OTU (ovarian tumor) domain of ubiquitin thioesterase otulin and similar proteins; Otulin, also ...
 85-296 5.70e-67

OTU (ovarian tumor) domain of ubiquitin thioesterase otulin and similar proteins; Otulin, also called FAM105B, deubiquitinating enzyme otulin, OTU domain-containing deubiquitinase with linear linkage specificity, or ubiquitin thioesterase Gumby, is a deubiquitinase (DUB)/ubiquitin thioesterase (EC 3.4.19.12) that specifically removes linear ('Met-1'-linked) polyubiquitin chains to substrates and acts as a regulator of angiogenesis and innate immune response. It acts as a key negative regulator of inflammation by restricting spontaneous inflammation and maintaining immune homeostasis. Otulin belongs to the OTU family of cysteine proteases that use a conserved cysteine and histidine, and in some cases an aspartate, as the catalytic dyad (or triad).


Pssm-ID: 438620  Cd Length: 266  Bit Score: 210.76  E-value: 5.70e-67
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 334848158  85 LSVEAEVDLLSYCAREWKGEAPRARLMRKAYEELfwRHHIKCVRAVKRDNYDALRSVLFQIFSQGLSFPSWMKEKDIVKL 164
Cdd:cd22799    1 LSVAPEMDILDYCKKEWRGNTQKATCMKKGYEEV--SQKFTSIRRVRGDNYCALRATLFQALSQAVGLPPWLQDPELMLL 78

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 334848158 165 PEKLLFSQgcNWIQQYSFGPEKYTGS-NVFGKLRKCVELLKLQWTEFSGMRDHHKRGSMCNSLFSDAILECKLYEALKFL 243
Cdd:cd22799   79 PEKLISKY--NWIKQWKLGLKFDGKNeDLVDKLKEYLTLLKKKWAGLAEMRTAEERQIACDELFTNEAEEYSLYEAVKFL 156

                        170       180       190       200       210
                 ....*....|....*....|....*....|....*....|....*....|...
gi 334848158 244 MLYQVTEAYEQMKTNKVIPSLFRLLFSRESSPDPLSFMMNHLNSIGDTCGLDQ 296
Cdd:cd22799  157 MLNRAIELYNDKEKGKEVPFFSWLLFARDTSNNPGQLLRNHLNQVGHSGGLEQ 209
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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