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Conserved domains on  [gi|334184259|ref|NP_001189536|]
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Iron-sulfur cluster biosynthesis family protein [Arabidopsis thaliana]

Protein Classification

HesB/IscA family protein( domain architecture ID 10001059)

HesB/IscA family protein is a scaffold protein upon which 2Fe-2S clusters are assembled and subsequently transferred to acceptor proteins; similar to iron-sulfur assembly protein IscA that is involved in the maturation of mitochondrial 4Fe-4S proteins functioning late in the iron-sulfur cluster assembly pathway

Gene Ontology:  GO:0016226|GO:0051536
PubMed:  32108236

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
IscA COG0316
Fe-S cluster assembly iron-binding protein IscA [Posttranslational modification, protein ...
 22-130 6.70e-44

Fe-S cluster assembly iron-binding protein IscA [Posttranslational modification, protein turnover, chaperones];


:

Pssm-ID: 440085 [Multi-domain]  Cd Length: 107  Bit Score: 139.52  E-value: 6.70e-44
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 334184259  22 VLTLTDEAASRVHHLLQQRQKP--FLRLGVKARGCNGLSYTLNYAgktsgnDEKGKFDELVEEKGVRILVEPKALMHVIG 99
Cdd:COG0316    1 PITLTDAAAKRIKRLLAKEGNPglGLRVGVKGGGCSGFSYGLDFD------DEPNEDDLVFEQDGVKVVVDPKSLPYLDG 74

                         90       100       110
                 ....*....|....*....|....*....|.
gi 334184259 100 TKMDFVDDKLRSEFVFINPNSQGQCGCGESF 130
Cdd:COG0316   75 TEIDYVEELLGSGFKFNNPNAKSSCGCGESF 105
 
Name Accession Description Interval E-value
IscA COG0316
Fe-S cluster assembly iron-binding protein IscA [Posttranslational modification, protein ...
 22-130 6.70e-44

Fe-S cluster assembly iron-binding protein IscA [Posttranslational modification, protein turnover, chaperones];


Pssm-ID: 440085 [Multi-domain]  Cd Length: 107  Bit Score: 139.52  E-value: 6.70e-44
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 334184259  22 VLTLTDEAASRVHHLLQQRQKP--FLRLGVKARGCNGLSYTLNYAgktsgnDEKGKFDELVEEKGVRILVEPKALMHVIG 99
Cdd:COG0316    1 PITLTDAAAKRIKRLLAKEGNPglGLRVGVKGGGCSGFSYGLDFD------DEPNEDDLVFEQDGVKVVVDPKSLPYLDG 74

                         90       100       110
                 ....*....|....*....|....*....|.
gi 334184259 100 TKMDFVDDKLRSEFVFINPNSQGQCGCGESF 130
Cdd:COG0316   75 TEIDYVEELLGSGFKFNNPNAKSSCGCGESF 105
TIGR00049 TIGR00049
Iron-sulfur cluster assembly accessory protein; Proteins in this subfamily appear to be ...
 24-132 1.06e-42

Iron-sulfur cluster assembly accessory protein; Proteins in this subfamily appear to be associated with the process of FeS-cluster assembly. The HesB proteins are associated with the nif gene cluster and the Rhizobium gene IscN has been shown to be required for nitrogen fixation. Nitrogenase includes multiple FeS clusters and many genes for their assembly. The E. coli SufA protein is associated with SufS, a NifS homolog and SufD which are involved in the FeS cluster assembly of the FhnF protein. The Azotobacter protein IscA (homologs of which are also found in E.coli) is associated which IscS, another NifS homolog and IscU, a nifU homolog as well as other factors consistent with a role in FeS cluster chemistry. A homolog from Geobacter contains a selenocysteine in place of an otherwise invariant cysteine, further suggesting a role in redox chemistry. [Biosynthesis of cofactors, prosthetic groups, and carriers, Other]


Pssm-ID: 272875 [Multi-domain]  Cd Length: 105  Bit Score: 136.56  E-value: 1.06e-42
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 334184259   24 TLTDEAASRVHHLLQQRQKP--FLRLGVKARGCNGLSYTLNYAgktsgnDEKGKFDELVEEKGVRILVEPKALMHVIGTK 101
Cdd:TIGR00049   1 TLTDSAAKRIKALLAGEGEPnlGLRVGVKGGGCSGLQYGLEFD------DEPNEDDEVFEQDGVKVVVDPKSLPYLDGSE 74

                          90       100       110
                  ....*....|....*....|....*....|.
gi 334184259  102 MDFVDDKLRSEFVFINPNSQGQCGCGESFMT 132
Cdd:TIGR00049  75 IDYVEELLGSGFTFTNPNAKGTCGCGKSFSV 105
iscA PRK09502
iron-sulfur cluster assembly protein IscA;
23-130 2.47e-27

iron-sulfur cluster assembly protein IscA;


Pssm-ID: 181914 [Multi-domain]  Cd Length: 107  Bit Score: 97.63  E-value: 2.47e-27
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 334184259  23 LTLTDEAASRVHHLLQQRQKPF-LRLGVKARGCNGLSYTLNYAgktsgnDEKGKFDELVEEKGVRILVEPKALMHVIGTK 101
Cdd:PRK09502   3 ITLSDSAAARVNTFLANRGKGFgLRLGVRTSGCSGMAYVLEFV------DEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQ 76

                         90       100
                 ....*....|....*....|....*....
gi 334184259 102 MDFVDDKLRSEFVFINPNSQGQCGCGESF 130
Cdd:PRK09502  77 LDFVKEGLNEGFKFTNPNVKDECGCGESF 105
Fe-S_biosyn pfam01521
Iron-sulphur cluster biosynthesis; This family is involved in iron-sulphur cluster ...
 23-128 2.02e-12

Iron-sulphur cluster biosynthesis; This family is involved in iron-sulphur cluster biosynthesis. Its members include proteins that are involved in nitrogen fixation such as the HesB and HesB-like proteins.


Pssm-ID: 426304  Cd Length: 111  Bit Score: 59.58  E-value: 2.02e-12
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 334184259   23 LTLTDEAASRVHHLLQQRQKPfLRLGVK------ARGC--NGLSYTLNYAgktsgNDEKGKFDELVEEKGVRILVEPKAL 94
Cdd:pfam01521   3 ITITDAAAERLKKLLAGDKKE-LRLDVDdgggpySKGGcsIGGKFSLVLV-----DEPDPDYDEVIESNGGPIYVDSYSL 76

                          90       100       110
                  ....*....|....*....|....*....|....*
gi 334184259   95 MHV-IGTKMDFVDDKLRSEFVFINPNSQGQCGCGE 128
Cdd:pfam01521  77 PFLdEGLTLDFVEDLGTLGLKSDNGNLDGNVGCGD 111
 
Name Accession Description Interval E-value
IscA COG0316
Fe-S cluster assembly iron-binding protein IscA [Posttranslational modification, protein ...
 22-130 6.70e-44

Fe-S cluster assembly iron-binding protein IscA [Posttranslational modification, protein turnover, chaperones];


Pssm-ID: 440085 [Multi-domain]  Cd Length: 107  Bit Score: 139.52  E-value: 6.70e-44
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 334184259  22 VLTLTDEAASRVHHLLQQRQKP--FLRLGVKARGCNGLSYTLNYAgktsgnDEKGKFDELVEEKGVRILVEPKALMHVIG 99
Cdd:COG0316    1 PITLTDAAAKRIKRLLAKEGNPglGLRVGVKGGGCSGFSYGLDFD------DEPNEDDLVFEQDGVKVVVDPKSLPYLDG 74

                         90       100       110
                 ....*....|....*....|....*....|.
gi 334184259 100 TKMDFVDDKLRSEFVFINPNSQGQCGCGESF 130
Cdd:COG0316   75 TEIDYVEELLGSGFKFNNPNAKSSCGCGESF 105
TIGR00049 TIGR00049
Iron-sulfur cluster assembly accessory protein; Proteins in this subfamily appear to be ...
 24-132 1.06e-42

Iron-sulfur cluster assembly accessory protein; Proteins in this subfamily appear to be associated with the process of FeS-cluster assembly. The HesB proteins are associated with the nif gene cluster and the Rhizobium gene IscN has been shown to be required for nitrogen fixation. Nitrogenase includes multiple FeS clusters and many genes for their assembly. The E. coli SufA protein is associated with SufS, a NifS homolog and SufD which are involved in the FeS cluster assembly of the FhnF protein. The Azotobacter protein IscA (homologs of which are also found in E.coli) is associated which IscS, another NifS homolog and IscU, a nifU homolog as well as other factors consistent with a role in FeS cluster chemistry. A homolog from Geobacter contains a selenocysteine in place of an otherwise invariant cysteine, further suggesting a role in redox chemistry. [Biosynthesis of cofactors, prosthetic groups, and carriers, Other]


Pssm-ID: 272875 [Multi-domain]  Cd Length: 105  Bit Score: 136.56  E-value: 1.06e-42
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 334184259   24 TLTDEAASRVHHLLQQRQKP--FLRLGVKARGCNGLSYTLNYAgktsgnDEKGKFDELVEEKGVRILVEPKALMHVIGTK 101
Cdd:TIGR00049   1 TLTDSAAKRIKALLAGEGEPnlGLRVGVKGGGCSGLQYGLEFD------DEPNEDDEVFEQDGVKVVVDPKSLPYLDGSE 74

                          90       100       110
                  ....*....|....*....|....*....|.
gi 334184259  102 MDFVDDKLRSEFVFINPNSQGQCGCGESFMT 132
Cdd:TIGR00049  75 IDYVEELLGSGFTFTNPNAKGTCGCGKSFSV 105
sufA_proteo TIGR01997
FeS assembly scaffold SufA; This model represents the SufA protein of the SUF system of ...
 21-130 6.73e-31

FeS assembly scaffold SufA; This model represents the SufA protein of the SUF system of iron-sulfur cluster biosynthesis. This system performs FeS biosynthesis even during oxidative stress and tends to be absent in obligate anaerobic and microaerophilic bacteria. [Biosynthesis of cofactors, prosthetic groups, and carriers, Other]


Pssm-ID: 131052  Cd Length: 107  Bit Score: 106.84  E-value: 6.73e-31
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 334184259   21 QVLTLTDEAASRVHHLLQQRQKP-FLRLGVKARGCNGLSYTLNYAgktsgnDEKGKFDELVEEKGVRILVEPKALMHVIG 99
Cdd:TIGR01997   1 AVITLTDAAAIHIRELVAKRPEAvGIRLGVKKTGCAGMEYVLDLV------SEPKKDDDLIEHDGAKVFVAPEAVLFILG 74

                          90       100       110
                  ....*....|....*....|....*....|.
gi 334184259  100 TKMDFVDDKLRSEFVFINPNSQGQCGCGESF 130
Cdd:TIGR01997  75 TQVDFVRTTLRQGFKFNNPNATSACGCGESF 105
IscA TIGR02011
iron-sulfur cluster assembly protein IscA; This model represents the IscA component of the ISC ...
 23-130 1.58e-28

iron-sulfur cluster assembly protein IscA; This model represents the IscA component of the ISC system for iron-sulfur cluster assembly. The ISC system consists of IscRASU, HscAB and an Isc-specific ferredoxin. IscA previously was believed to act as a scaffold and now is seen as an iron donor protein. This clade is limited to the proteobacteria. [Biosynthesis of cofactors, prosthetic groups, and carriers, Other]


Pssm-ID: 213674  Cd Length: 105  Bit Score: 100.67  E-value: 1.58e-28
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 334184259   23 LTLTDEAASRVHHLLQQRQKPF-LRLGVKARGCNGLSYTLNYAgktsgnDEKGKFDELVEEKGVRILVEPKALMHVIGTK 101
Cdd:TIGR02011   1 ITLTDSAAARVNTFLANRGKGFgLRLGVKTSGCSGMAYVLEFV------DEPTPDDIVFEDKGVKIVIDGKSLQYLDGTQ 74

                          90       100
                  ....*....|....*....|....*....
gi 334184259  102 MDFVDDKLRSEFVFINPNSQGQCGCGESF 130
Cdd:TIGR02011  75 LDFVKEGLNEGFKFTNPNVKDECGCGESF 103
iscA PRK09502
iron-sulfur cluster assembly protein IscA;
23-130 2.47e-27

iron-sulfur cluster assembly protein IscA;


Pssm-ID: 181914 [Multi-domain]  Cd Length: 107  Bit Score: 97.63  E-value: 2.47e-27
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 334184259  23 LTLTDEAASRVHHLLQQRQKPF-LRLGVKARGCNGLSYTLNYAgktsgnDEKGKFDELVEEKGVRILVEPKALMHVIGTK 101
Cdd:PRK09502   3 ITLSDSAAARVNTFLANRGKGFgLRLGVRTSGCSGMAYVLEFV------DEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQ 76

                         90       100
                 ....*....|....*....|....*....
gi 334184259 102 MDFVDDKLRSEFVFINPNSQGQCGCGESF 130
Cdd:PRK09502  77 LDFVKEGLNEGFKFTNPNVKDECGCGESF 105
sufA PRK09504
iron-sulfur cluster assembly scaffold protein; Provisional
 21-130 1.86e-23

iron-sulfur cluster assembly scaffold protein; Provisional


Pssm-ID: 181915  Cd Length: 122  Bit Score: 88.26  E-value: 1.86e-23
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 334184259  21 QVLTLTDEAASRVHHLLQQR-QKPFLRLGVKARGCNGLSYTLNYAgktsgnDEKGKFDELVEEKGVRILVEPKALMHVIG 99
Cdd:PRK09504  16 QGLTLTPAAAAHIRELMAKQpGMKGVRLGVKQTGCAGFGYVLDSV------SEPDKDDLVFEHDGAKLFVPLQAMPFIDG 89

                         90       100       110
                 ....*....|....*....|....*....|.
gi 334184259 100 TKMDFVDDKLRSEFVFINPNSQGQCGCGESF 130
Cdd:PRK09504  90 TEVDYVREGLNQIFKFHNPKAQNECGCGESF 120
PRK13623 PRK13623
iron-sulfur cluster insertion protein ErpA; Provisional
 23-130 5.47e-18

iron-sulfur cluster insertion protein ErpA; Provisional


Pssm-ID: 184186  Cd Length: 115  Bit Score: 74.19  E-value: 5.47e-18
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 334184259  23 LTLTDEAASRVHHLLQQRQKPFLRLGV--KARGCNGLSYTLNYagktsgnDEKGKFDELVEEK-GVRILVEPKALMHVIG 99
Cdd:PRK13623  10 LVFTDAAAAKVKELIEEEGNPDLKLRVyiTGGGCSGFQYGFTF-------DEQVNEDDTTIEKqGVTLVVDPMSLQYLVG 82

                         90       100       110
                 ....*....|....*....|....*....|.
gi 334184259 100 TKMDFVDDKLRSEFVFINPNSQGQCGCGESF 130
Cdd:PRK13623  83 AEVDYTEGLEGSRFVIKNPNAKTTCGCGSSF 113
PLN03082 PLN03082
Iron-sulfur cluster assembly; Provisional
 21-131 2.79e-13

Iron-sulfur cluster assembly; Provisional


Pssm-ID: 215564  Cd Length: 163  Bit Score: 63.02  E-value: 2.79e-13
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 334184259  21 QVLTLTDEAASRVHHLLQQR---QKPFLRLGVKARGCNGLSYTLNYAGKTSGNdekgkfDELVEEKGVRILVEPKALMHV 97
Cdd:PLN03082  53 DAVHMTDNCIRRLKELQTSEpsaEDKMLRLSVETGGCSGFQYVFELDDKTNSD------DRVFEKDGVKLVVDNISYDFV 126

                         90       100       110
                 ....*....|....*....|....*....|....*
gi 334184259  98 IGTKMDFVDDKLRSEFVF-INPNSQGQCGCGESFM 131
Cdd:PLN03082 127 KGATVDYVEELIRSAFVVsTNPSAVGGCSCKSSFM 161
Fe-S_biosyn pfam01521
Iron-sulphur cluster biosynthesis; This family is involved in iron-sulphur cluster ...
 23-128 2.02e-12

Iron-sulphur cluster biosynthesis; This family is involved in iron-sulphur cluster biosynthesis. Its members include proteins that are involved in nitrogen fixation such as the HesB and HesB-like proteins.


Pssm-ID: 426304  Cd Length: 111  Bit Score: 59.58  E-value: 2.02e-12
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 334184259   23 LTLTDEAASRVHHLLQQRQKPfLRLGVK------ARGC--NGLSYTLNYAgktsgNDEKGKFDELVEEKGVRILVEPKAL 94
Cdd:pfam01521   3 ITITDAAAERLKKLLAGDKKE-LRLDVDdgggpySKGGcsIGGKFSLVLV-----DEPDPDYDEVIESNGGPIYVDSYSL 76

                          90       100       110
                  ....*....|....*....|....*....|....*
gi 334184259   95 MHV-IGTKMDFVDDKLRSEFVFINPNSQGQCGCGE 128
Cdd:pfam01521  77 PFLdEGLTLDFVEDLGTLGLKSDNGNLDGNVGCGD 111
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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