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Conserved domains on  [gi|269847404|ref|NP_001161413|]
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WD repeat-containing protein 76 isoform 2 [Homo sapiens]

Protein Classification

WD40 repeat domain-containing protein( domain architecture ID 1000017)

WD40 repeat domain-containing protein folds into a beta-propeller structure and functions as a scaffold, providing a platform for the interaction and assembly of several proteins into a signalosome; similar to a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly

CATH:  2.130.10.10
Gene Ontology:  GO:0005515
PubMed:  10322433|8090199|30069656|29026209
SCOP:  4002744

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
WD40 super family cl29593
WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions ...
 249-460 4.52e-13

WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from its N-terminus and the WD dipeptide at its C-terminus and is 40 residues long, hence the name WD40; between GH and WD lies a conserved core; serves as a stable propeller-like platform to which proteins can bind either stably or reversibly; forms a propeller-like structure with several blades where each blade is composed of a four-stranded anti-parallel b-sheet; instances with few detectable copies are hypothesized to form larger structures by dimerization; each WD40 sequence repeat forms the first three strands of one blade and the last strand in the next blade; the last C-terminal WD40 repeat completes the blade structure of the first WD40 repeat to create the closed ring propeller-structure; residues on the top and bottom surface of the propeller are proposed to coordinate interactions with other proteins and/or small ligands; 7 copies of the repeat are present in this alignment.


The actual alignment was detected with superfamily member cd00200:

Pssm-ID: 475233 [Multi-domain]  Cd Length: 289  Bit Score: 70.06  E-value: 4.52e-13
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 269847404 249 TGPIFSMALHPSetRTLVAVGAKFGQVGLCDLtqqPKEDGVYVFHPHSQPVSCLYFSPANPaHILSLSYDGTLRCGDFSR 328
Cdd:cd00200   93 TSYVSSVAFSPD--GRILSSSSRDKTIKVWDV---ETGKCLTTLRGHTDWVNSVAFSPDGT-FVASSSQDGTIKLWDLRT 166

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 269847404 329 AIFEEVYRNERSSFSSFDFLaEDASTLIVGHWDGNMSLVDRRTpGTSYEKLTSSSMGkIRTVHVHPvhRQYFITAGLRDT 408
Cdd:cd00200  167 GKCVATLTGHTGEVNSVAFS-PDGEKLLSSSSDGTIKLWDLST-GKCLGTLRGHENG-VNSVAFSP--DGYLLASGSEDG 241

                        170       180       190       200       210
                 ....*....|....*....|....*....|....*....|....*....|...
gi 269847404 409 HIydarRL-NSRRSQPLISLTEHTKSIASAYFSPlTGNRVVTTCADCNLRIFD 460
Cdd:cd00200  242 TI----RVwDLRTGECVQTLSGHTNSVTSLAWSP-DGKRLASGSADGTIRIWD 289
 
Name Accession Description Interval E-value
WD40 cd00200
WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions ...
 249-460 4.52e-13

WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from its N-terminus and the WD dipeptide at its C-terminus and is 40 residues long, hence the name WD40; between GH and WD lies a conserved core; serves as a stable propeller-like platform to which proteins can bind either stably or reversibly; forms a propeller-like structure with several blades where each blade is composed of a four-stranded anti-parallel b-sheet; instances with few detectable copies are hypothesized to form larger structures by dimerization; each WD40 sequence repeat forms the first three strands of one blade and the last strand in the next blade; the last C-terminal WD40 repeat completes the blade structure of the first WD40 repeat to create the closed ring propeller-structure; residues on the top and bottom surface of the propeller are proposed to coordinate interactions with other proteins and/or small ligands; 7 copies of the repeat are present in this alignment.


Pssm-ID: 238121 [Multi-domain]  Cd Length: 289  Bit Score: 70.06  E-value: 4.52e-13
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 269847404 249 TGPIFSMALHPSetRTLVAVGAKFGQVGLCDLtqqPKEDGVYVFHPHSQPVSCLYFSPANPaHILSLSYDGTLRCGDFSR 328
Cdd:cd00200   93 TSYVSSVAFSPD--GRILSSSSRDKTIKVWDV---ETGKCLTTLRGHTDWVNSVAFSPDGT-FVASSSQDGTIKLWDLRT 166

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 269847404 329 AIFEEVYRNERSSFSSFDFLaEDASTLIVGHWDGNMSLVDRRTpGTSYEKLTSSSMGkIRTVHVHPvhRQYFITAGLRDT 408
Cdd:cd00200  167 GKCVATLTGHTGEVNSVAFS-PDGEKLLSSSSDGTIKLWDLST-GKCLGTLRGHENG-VNSVAFSP--DGYLLASGSEDG 241

                        170       180       190       200       210
                 ....*....|....*....|....*....|....*....|....*....|...
gi 269847404 409 HIydarRL-NSRRSQPLISLTEHTKSIASAYFSPlTGNRVVTTCADCNLRIFD 460
Cdd:cd00200  242 TI----RVwDLRTGECVQTLSGHTNSVTSLAWSP-DGKRLASGSADGTIRIWD 289
WD40 COG2319
WD40 repeat [General function prediction only];
249-460 9.11e-12

WD40 repeat [General function prediction only];


Pssm-ID: 441893 [Multi-domain]  Cd Length: 403  Bit Score: 66.86  E-value: 9.11e-12
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 269847404 249 TGPIFSMALHPSETRtlVAVGAKFGQVGLCDLTQQPKedgVYVFHPHSQPVSCLYFSPaNPAHILSLSYDGTLRCGDFSR 328
Cdd:COG2319  204 TGAVRSVAFSPDGKL--LASGSADGTVRLWDLATGKL---LRTLTGHSGSVRSVAFSP-DGRLLASGSADGTVRLWDLAT 277

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 269847404 329 AIFEEVYRNERSSFSSFDFLAeDASTLIVGHWDGNMSLVDRRTpGTSYEKLTSSSmGKIRTVHVHPvHRQYFITAGLRDT 408
Cdd:COG2319  278 GELLRTLTGHSGGVNSVAFSP-DGKLLASGSDDGTVRLWDLAT-GKLLRTLTGHT-GAVRSVAFSP-DGKTLASGSDDGT 353

                        170       180       190       200       210
                 ....*....|....*....|....*....|....*....|....*....|..
gi 269847404 409 hiydARRLNSRRSQPLISLTEHTKSIASAYFSPlTGNRVVTTCADCNLRIFD 460
Cdd:COG2319  354 ----VRLWDLATGELLRTLTGHTGAVTSVAFSP-DGRTLASGSADGTVRLWD 400
WD40 smart00320
WD40 repeats; Note that these repeats are permuted with respect to the structural repeats ...
 422-460 1.85e-03

WD40 repeats; Note that these repeats are permuted with respect to the structural repeats (blades) of the beta propeller domain.


Pssm-ID: 197651 [Multi-domain]  Cd Length: 40  Bit Score: 36.14  E-value: 1.85e-03
                           10        20        30
                   ....*....|....*....|....*....|....*....
gi 269847404   422 QPLISLTEHTKSIASAYFSPlTGNRVVTTCADCNLRIFD 460
Cdd:smart00320   3 ELLKTLKGHTGPVTSVAFSP-DGKYLASGSDDGTIKLWD 40
WD40 pfam00400
WD domain, G-beta repeat;
421-460 6.01e-03

WD domain, G-beta repeat;


Pssm-ID: 459801 [Multi-domain]  Cd Length: 39  Bit Score: 34.63  E-value: 6.01e-03
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|
gi 269847404  421 SQPLISLTEHTKSIASAYFSPlTGNRVVTTCADCNLRIFD 460
Cdd:pfam00400   1 GKLLKTLEGHTGSVTSLAFSP-DGKLLASGSDDGTVKVWD 39
 
Name Accession Description Interval E-value
WD40 cd00200
WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions ...
 249-460 4.52e-13

WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from its N-terminus and the WD dipeptide at its C-terminus and is 40 residues long, hence the name WD40; between GH and WD lies a conserved core; serves as a stable propeller-like platform to which proteins can bind either stably or reversibly; forms a propeller-like structure with several blades where each blade is composed of a four-stranded anti-parallel b-sheet; instances with few detectable copies are hypothesized to form larger structures by dimerization; each WD40 sequence repeat forms the first three strands of one blade and the last strand in the next blade; the last C-terminal WD40 repeat completes the blade structure of the first WD40 repeat to create the closed ring propeller-structure; residues on the top and bottom surface of the propeller are proposed to coordinate interactions with other proteins and/or small ligands; 7 copies of the repeat are present in this alignment.


Pssm-ID: 238121 [Multi-domain]  Cd Length: 289  Bit Score: 70.06  E-value: 4.52e-13
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 269847404 249 TGPIFSMALHPSetRTLVAVGAKFGQVGLCDLtqqPKEDGVYVFHPHSQPVSCLYFSPANPaHILSLSYDGTLRCGDFSR 328
Cdd:cd00200   93 TSYVSSVAFSPD--GRILSSSSRDKTIKVWDV---ETGKCLTTLRGHTDWVNSVAFSPDGT-FVASSSQDGTIKLWDLRT 166

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 269847404 329 AIFEEVYRNERSSFSSFDFLaEDASTLIVGHWDGNMSLVDRRTpGTSYEKLTSSSMGkIRTVHVHPvhRQYFITAGLRDT 408
Cdd:cd00200  167 GKCVATLTGHTGEVNSVAFS-PDGEKLLSSSSDGTIKLWDLST-GKCLGTLRGHENG-VNSVAFSP--DGYLLASGSEDG 241

                        170       180       190       200       210
                 ....*....|....*....|....*....|....*....|....*....|...
gi 269847404 409 HIydarRL-NSRRSQPLISLTEHTKSIASAYFSPlTGNRVVTTCADCNLRIFD 460
Cdd:cd00200  242 TI----RVwDLRTGECVQTLSGHTNSVTSLAWSP-DGKRLASGSADGTIRIWD 289
WD40 cd00200
WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions ...
 295-556 1.22e-12

WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from its N-terminus and the WD dipeptide at its C-terminus and is 40 residues long, hence the name WD40; between GH and WD lies a conserved core; serves as a stable propeller-like platform to which proteins can bind either stably or reversibly; forms a propeller-like structure with several blades where each blade is composed of a four-stranded anti-parallel b-sheet; instances with few detectable copies are hypothesized to form larger structures by dimerization; each WD40 sequence repeat forms the first three strands of one blade and the last strand in the next blade; the last C-terminal WD40 repeat completes the blade structure of the first WD40 repeat to create the closed ring propeller-structure; residues on the top and bottom surface of the propeller are proposed to coordinate interactions with other proteins and/or small ligands; 7 copies of the repeat are present in this alignment.


Pssm-ID: 238121 [Multi-domain]  Cd Length: 289  Bit Score: 68.52  E-value: 1.22e-12
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 269847404 295 HSQPVSCLYFSPANPaHILSLSYDGTLRCGDFSRAIFEEVYRNERSSFSSFDFLAeDASTLIVGHWDGNMSLVDRRTPGT 374
Cdd:cd00200    8 HTGGVTCVAFSPDGK-LLATGSGDGTIKVWDLETGELLRTLKGHTGPVRDVAASA-DGTYLASGSSDKTIRLWDLETGEC 85

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 269847404 375 SYEkLTSSSmGKIRTVHVHPVHRqyFITAGLRD--THIYDARRlnsrrSQPLISLTEHTKSIASAYFSPlTGNRVVTTCA 452
Cdd:cd00200   86 VRT-LTGHT-SYVSSVAFSPDGR--ILSSSSRDktIKVWDVET-----GKCLTTLRGHTDWVNSVAFSP-DGTFVASSSQ 155

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 269847404 453 DCNLRIFDSSCISskipLLTTIRHNTFTGRWLTrfqamWDPKQEDCVIVGSMAHPRRVEIfhETGKRVHSFGGEyLVSVC 532
Cdd:cd00200  156 DGTIKLWDLRTGK----CVATLTGHTGEVNSVA-----FSPDGEKLLSSSSDGTIKLWDL--STGKCLGTLRGH-ENGVN 223

                        250       260
                 ....*....|....*....|....
gi 269847404 533 SInAMHPTRYILAGGNSSGKIHVF 556
Cdd:cd00200  224 SV-AFSPDGYLLASGSEDGTIRVW 246
WD40 COG2319
WD40 repeat [General function prediction only];
249-460 9.11e-12

WD40 repeat [General function prediction only];


Pssm-ID: 441893 [Multi-domain]  Cd Length: 403  Bit Score: 66.86  E-value: 9.11e-12
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 269847404 249 TGPIFSMALHPSETRtlVAVGAKFGQVGLCDLTQQPKedgVYVFHPHSQPVSCLYFSPaNPAHILSLSYDGTLRCGDFSR 328
Cdd:COG2319  204 TGAVRSVAFSPDGKL--LASGSADGTVRLWDLATGKL---LRTLTGHSGSVRSVAFSP-DGRLLASGSADGTVRLWDLAT 277

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 269847404 329 AIFEEVYRNERSSFSSFDFLAeDASTLIVGHWDGNMSLVDRRTpGTSYEKLTSSSmGKIRTVHVHPvHRQYFITAGLRDT 408
Cdd:COG2319  278 GELLRTLTGHSGGVNSVAFSP-DGKLLASGSDDGTVRLWDLAT-GKLLRTLTGHT-GAVRSVAFSP-DGKTLASGSDDGT 353

                        170       180       190       200       210
                 ....*....|....*....|....*....|....*....|....*....|..
gi 269847404 409 hiydARRLNSRRSQPLISLTEHTKSIASAYFSPlTGNRVVTTCADCNLRIFD 460
Cdd:COG2319  354 ----VRLWDLATGELLRTLTGHTGAVTSVAFSP-DGRTLASGSADGTVRLWD 400
WD40 COG2319
WD40 repeat [General function prediction only];
249-460 7.29e-11

WD40 repeat [General function prediction only];


Pssm-ID: 441893 [Multi-domain]  Cd Length: 403  Bit Score: 64.16  E-value: 7.29e-11
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 269847404 249 TGPIFSMALHPSETRtlVAVGAKFGQVGLCDLTQQPKedgVYVFHPHSQPVSCLYFSPaNPAHILSLSYDGTLRCGDFSR 328
Cdd:COG2319  120 TGAVRSVAFSPDGKT--LASGSADGTVRLWDLATGKL---LRTLTGHSGAVTSVAFSP-DGKLLASGSDDGTVRLWDLAT 193

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 269847404 329 AIFEEVYRNERSSFSSFDFlAEDASTLIVGHWDGNMSLVDRRTpGTSYEKLTSSSmGKIRTVHVHPvHRQYFITAGLRDT 408
Cdd:COG2319  194 GKLLRTLTGHTGAVRSVAF-SPDGKLLASGSADGTVRLWDLAT-GKLLRTLTGHS-GSVRSVAFSP-DGRLLASGSADGT 269

                        170       180       190       200       210
                 ....*....|....*....|....*....|....*....|....*....|...
gi 269847404 409 -HIYDArrlnsRRSQPLISLTEHTKSIASAYFSPlTGNRVVTTCADCNLRIFD 460
Cdd:COG2319  270 vRLWDL-----ATGELLRTLTGHSGGVNSVAFSP-DGKLLASGSDDGTVRLWD 316
WD40 COG2319
WD40 repeat [General function prediction only];
291-556 8.10e-11

WD40 repeat [General function prediction only];


Pssm-ID: 441893 [Multi-domain]  Cd Length: 403  Bit Score: 64.16  E-value: 8.10e-11
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 269847404 291 VFHPHSQPVSCLYFSPaNPAHILSLSYDGTLRCGDFSRAIFEEVYRNERSSFSSFDFlAEDASTLIVGHWDGNMSLVDRR 370
Cdd:COG2319   73 TLLGHTAAVLSVAFSP-DGRLLASASADGTVRLWDLATGLLLRTLTGHTGAVRSVAF-SPDGKTLASGSADGTVRLWDLA 150

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 269847404 371 TpGTSYEKLTSSSmGKIRTVHVHPvHRQYFITAGLRDThiydARRLNSRRSQPLISLTEHTKSIASAYFSPlTGNRVVTT 450
Cdd:COG2319  151 T-GKLLRTLTGHS-GAVTSVAFSP-DGKLLASGSDDGT----VRLWDLATGKLLRTLTGHTGAVRSVAFSP-DGKLLASG 222

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 269847404 451 CADCNLRIFDsscISSKiPLLTTIRHNTFT---------GRWLtrfqamwdpkqedcvIVGSmaHPRRVEIFH-ETGKRV 520
Cdd:COG2319  223 SADGTVRLWD---LATG-KLLRTLTGHSGSvrsvafspdGRLL---------------ASGS--ADGTVRLWDlATGELL 281

                        250       260       270
                 ....*....|....*....|....*....|....*.
gi 269847404 521 HSFGGEyLVSVCSInAMHPTRYILAGGNSSGKIHVF 556
Cdd:COG2319  282 RTLTGH-SGGVNSV-AFSPDGKLLASGSDDGTVRLW 315
WD40 smart00320
WD40 repeats; Note that these repeats are permuted with respect to the structural repeats ...
 422-460 1.85e-03

WD40 repeats; Note that these repeats are permuted with respect to the structural repeats (blades) of the beta propeller domain.


Pssm-ID: 197651 [Multi-domain]  Cd Length: 40  Bit Score: 36.14  E-value: 1.85e-03
                           10        20        30
                   ....*....|....*....|....*....|....*....
gi 269847404   422 QPLISLTEHTKSIASAYFSPlTGNRVVTTCADCNLRIFD 460
Cdd:smart00320   3 ELLKTLKGHTGPVTSVAFSP-DGKYLASGSDDGTIKLWD 40
WD40 smart00320
WD40 repeats; Note that these repeats are permuted with respect to the structural repeats ...
 289-323 4.68e-03

WD40 repeats; Note that these repeats are permuted with respect to the structural repeats (blades) of the beta propeller domain.


Pssm-ID: 197651 [Multi-domain]  Cd Length: 40  Bit Score: 34.98  E-value: 4.68e-03
                           10        20        30
                   ....*....|....*....|....*....|....*
gi 269847404   289 VYVFHPHSQPVSCLYFSPANPaHILSLSYDGTLRC 323
Cdd:smart00320   5 LKTLKGHTGPVTSVAFSPDGK-YLASGSDDGTIKL 38
WD40 pfam00400
WD domain, G-beta repeat;
421-460 6.01e-03

WD domain, G-beta repeat;


Pssm-ID: 459801 [Multi-domain]  Cd Length: 39  Bit Score: 34.63  E-value: 6.01e-03
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|
gi 269847404  421 SQPLISLTEHTKSIASAYFSPlTGNRVVTTCADCNLRIFD 460
Cdd:pfam00400   1 GKLLKTLEGHTGSVTSLAFSP-DGKLLASGSDDGTVKVWD 39
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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