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Conserved domains on  [gi|254553280|ref|NP_001156948|]
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DNA replication complex GINS protein PSF1 isoform 2 [Mus musculus]

Protein Classification

DNA replication complex GINS protein PSF1 family protein( domain architecture ID 10184919)

DNA replication complex GINS protein PSF1 family protein similar to alpha-helical region of DNA replication complex GINS protein PSF1, which is a component of the heterotetrameric GINS complex essential for both the initiation and elongation stages of eukaryotic DNA replication

CATH:  1.20.58.1030
Gene Ontology:  GO:0006260
PubMed:  22918584|20070258

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
GINS_A_psf1 cd11710
Alpha-helical domain of GINS complex protein Psf1; Psf1 is a component of the GINS tetrameric ...
 3-130 5.44e-56

Alpha-helical domain of GINS complex protein Psf1; Psf1 is a component of the GINS tetrameric protein complex. Psf1 is mainly expressed in highly proliferative tissues, such as blastocysts, adult bone marrow, and testis, in which the stem cell system is active. Loss of Psf1 causes embryonic lethality. GINS is a complex of four subunits (Sld5, Psf1, Psf2 and Psf3) that is involved in both initiation and elongation stages of eukaryotic chromosome replication. Besides being essential for the maintenance of genomic integrity, GINS plays a central role in coordinating DNA replication with cell cycle checkpoints and is involved in cell growth. The eukaryotic GINS subunits are homologous and homologs are also found in the archaea; the complex is not found in bacteria. The four subunits of the complex consist of two domains each, termed the alpha-helical (A) and beta-strand (B) domains. The A and B domains of Sld5/Psf1 are permuted with respect to Psf1/Psf3.


:

Pssm-ID: 212548  Cd Length: 129  Bit Score: 172.05  E-value: 5.44e-56
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 254553280   3 CEKAMELVRELHRAPEGQLPAFNEDGLRQVLEEMKALYEQNQSDVNEAKSAGRGD-LIPTVKFRHCALLRNRRCTIAYLY 81
Cdd:cd11710    1 GDKANKLVKELKRSENDTLPPYNEDLVRQVLEEIRDLYEENQALLEEAQEEEEDPgLIPGLLVRHLSILRNKRCLLAYLY 80

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....*....
gi 254553280  82 DRLLRIRALRWEYGSVLPNSLRFHMSAEETEWFNHYKKSLATYMRSLGG 130
Cdd:cd11710   81 ERLDRIRELRWENGSVLPEDIKENLSPAEKEYFKQYSKLLAEYMSSLGD 129
 
Name Accession Description Interval E-value
GINS_A_psf1 cd11710
Alpha-helical domain of GINS complex protein Psf1; Psf1 is a component of the GINS tetrameric ...
 3-130 5.44e-56

Alpha-helical domain of GINS complex protein Psf1; Psf1 is a component of the GINS tetrameric protein complex. Psf1 is mainly expressed in highly proliferative tissues, such as blastocysts, adult bone marrow, and testis, in which the stem cell system is active. Loss of Psf1 causes embryonic lethality. GINS is a complex of four subunits (Sld5, Psf1, Psf2 and Psf3) that is involved in both initiation and elongation stages of eukaryotic chromosome replication. Besides being essential for the maintenance of genomic integrity, GINS plays a central role in coordinating DNA replication with cell cycle checkpoints and is involved in cell growth. The eukaryotic GINS subunits are homologous and homologs are also found in the archaea; the complex is not found in bacteria. The four subunits of the complex consist of two domains each, termed the alpha-helical (A) and beta-strand (B) domains. The A and B domains of Sld5/Psf1 are permuted with respect to Psf1/Psf3.


Pssm-ID: 212548  Cd Length: 129  Bit Score: 172.05  E-value: 5.44e-56
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 254553280   3 CEKAMELVRELHRAPEGQLPAFNEDGLRQVLEEMKALYEQNQSDVNEAKSAGRGD-LIPTVKFRHCALLRNRRCTIAYLY 81
Cdd:cd11710    1 GDKANKLVKELKRSENDTLPPYNEDLVRQVLEEIRDLYEENQALLEEAQEEEEDPgLIPGLLVRHLSILRNKRCLLAYLY 80

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....*....
gi 254553280  82 DRLLRIRALRWEYGSVLPNSLRFHMSAEETEWFNHYKKSLATYMRSLGG 130
Cdd:cd11710   81 ERLDRIRELRWENGSVLPEDIKENLSPAEKEYFKQYSKLLAEYMSSLGD 129
COG5230 COG5230
Uncharacterized conserved protein [Function unknown];
1-152 1.41e-12

Uncharacterized conserved protein [Function unknown];


Pssm-ID: 227555 [Multi-domain]  Cd Length: 194  Bit Score: 62.27  E-value: 1.41e-12
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 254553280   1 MFCEKAMELVRELHRAPE-GQLPAFNEDGLRQVLEEMKALYEQNQSDVNEAKSAGRGDLIPT----VKFRHCALlRNRRC 75
Cdd:COG5230    1 MFGELGNKLLLEDKRTDVlDPLPPYRRDEIKAIEGENEHLDRRMEEILQEASQAGSGIDEELsvnyVMMKYFKE-RNKRC 79

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 254553280  76 TIAYLYDRLLRIRALRWEYGSVLPNSLRFHMSAEETEWFNHYKKSLATYmrsLGGDEGLDITQDVKPPKSLYIEVCV 152
Cdd:COG5230   80 LRAYKFLRSAALFDSFWSKGKRMKSVGDTMLSHEEEEYLEKYSNLLREY---KGPFKHIDLTGSLVPPVQFFIDVRV 153
Sld5 pfam05916
GINS complex protein; The eukaryotic GINS complex is essential for the initiation and ...
 20-110 4.97e-05

GINS complex protein; The eukaryotic GINS complex is essential for the initiation and elongation phases of DNA replication. It consists of four paralogous protein subunits (Sld5, Psf1, Psf2 and Psf3), all of which are included in this family. The GINS complex is conserved from yeast to humans, and has been shown in human to bind directly to DNA primase.


Pssm-ID: 399129  Cd Length: 105  Bit Score: 40.56  E-value: 4.97e-05
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 254553280   20 QLPAFNEDGL-RQVLEEMKALYEQNQSDVNEAKSAGR----GDLIP---TVKFRHCALLRN---RRCTIAYLYDRLLRIR 88
Cdd:pfam05916   2 ELPLWLAELLkRRGLVEIEPPEWLSIEELEAILADETkndlSLLPPyfyELAVLLLELERDdelKRLLRDYLRIRLAKIR 81

                          90       100
                  ....*....|....*....|...
gi 254553280   89 ALRWEYGSVL-PNSLRFHMSAEE 110
Cdd:pfam05916  82 KLAWHLNGSLsPSDLLDNLSEEE 104
 
Name Accession Description Interval E-value
GINS_A_psf1 cd11710
Alpha-helical domain of GINS complex protein Psf1; Psf1 is a component of the GINS tetrameric ...
 3-130 5.44e-56

Alpha-helical domain of GINS complex protein Psf1; Psf1 is a component of the GINS tetrameric protein complex. Psf1 is mainly expressed in highly proliferative tissues, such as blastocysts, adult bone marrow, and testis, in which the stem cell system is active. Loss of Psf1 causes embryonic lethality. GINS is a complex of four subunits (Sld5, Psf1, Psf2 and Psf3) that is involved in both initiation and elongation stages of eukaryotic chromosome replication. Besides being essential for the maintenance of genomic integrity, GINS plays a central role in coordinating DNA replication with cell cycle checkpoints and is involved in cell growth. The eukaryotic GINS subunits are homologous and homologs are also found in the archaea; the complex is not found in bacteria. The four subunits of the complex consist of two domains each, termed the alpha-helical (A) and beta-strand (B) domains. The A and B domains of Sld5/Psf1 are permuted with respect to Psf1/Psf3.


Pssm-ID: 212548  Cd Length: 129  Bit Score: 172.05  E-value: 5.44e-56
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 254553280   3 CEKAMELVRELHRAPEGQLPAFNEDGLRQVLEEMKALYEQNQSDVNEAKSAGRGD-LIPTVKFRHCALLRNRRCTIAYLY 81
Cdd:cd11710    1 GDKANKLVKELKRSENDTLPPYNEDLVRQVLEEIRDLYEENQALLEEAQEEEEDPgLIPGLLVRHLSILRNKRCLLAYLY 80

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....*....
gi 254553280  82 DRLLRIRALRWEYGSVLPNSLRFHMSAEETEWFNHYKKSLATYMRSLGG 130
Cdd:cd11710   81 ERLDRIRELRWENGSVLPEDIKENLSPAEKEYFKQYSKLLAEYMSSLGD 129
GINS_A cd11581
Alpha-helical domain of GINS complex proteins; Sld5, Psf1, Psf2 and Psf3; The GINS complex is ...
 3-123 3.47e-25

Alpha-helical domain of GINS complex proteins; Sld5, Psf1, Psf2 and Psf3; The GINS complex is involved in both initiation and elongation stages of eukaryotic chromosome replication, with GINS being the component that most likely serves as the replicative helicase that unwinds duplex DNA ahead of the moving replication fork. In eukaryotes, GINS is a tetrameric arrangement of four subunits Sld5, Psf1, Psf2 and Psf3. The GINS complex has been found in eukaryotes and archaea, but not in bacteria. The four subunits of the complex are homologous and consist of two domains each, termed the alpha-helical (A) and beta-strand (B) domains. The A and B domains of Sld5/Psf1 are permuted with respect to Psf1/Psf3.


Pssm-ID: 212547  Cd Length: 103  Bit Score: 92.94  E-value: 3.47e-25
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 254553280   3 CEKAMELVRELHRapEGQLPAFNEDGLRQVLEEMKALYEQNQSDVNEaksagrgdlIPTVKFRHCALLRNRRCTIAYLYD 82
Cdd:cd11581    1 VEKLAEAIHNERK--SENLLPPPPDFMEEVKELLHRLSKRQRSETQE---------ILEHRLKILELSYIRDLLKTYLNS 69

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|.
gi 254553280  83 RLLRIRALRWEYgsvlpnsLRFHMSAEETEWFNHYKKSLAT 123
Cdd:cd11581   70 RLDKIRDLSPEF-------LPSNLTEEEIGYFQTYWKEVAE 103
COG5230 COG5230
Uncharacterized conserved protein [Function unknown];
1-152 1.41e-12

Uncharacterized conserved protein [Function unknown];


Pssm-ID: 227555 [Multi-domain]  Cd Length: 194  Bit Score: 62.27  E-value: 1.41e-12
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 254553280   1 MFCEKAMELVRELHRAPE-GQLPAFNEDGLRQVLEEMKALYEQNQSDVNEAKSAGRGDLIPT----VKFRHCALlRNRRC 75
Cdd:COG5230    1 MFGELGNKLLLEDKRTDVlDPLPPYRRDEIKAIEGENEHLDRRMEEILQEASQAGSGIDEELsvnyVMMKYFKE-RNKRC 79

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 254553280  76 TIAYLYDRLLRIRALRWEYGSVLPNSLRFHMSAEETEWFNHYKKSLATYmrsLGGDEGLDITQDVKPPKSLYIEVCV 152
Cdd:COG5230   80 LRAYKFLRSAALFDSFWSKGKRMKSVGDTMLSHEEEEYLEKYSNLLREY---KGPFKHIDLTGSLVPPVQFFIDVRV 153
Sld5 pfam05916
GINS complex protein; The eukaryotic GINS complex is essential for the initiation and ...
 20-110 4.97e-05

GINS complex protein; The eukaryotic GINS complex is essential for the initiation and elongation phases of DNA replication. It consists of four paralogous protein subunits (Sld5, Psf1, Psf2 and Psf3), all of which are included in this family. The GINS complex is conserved from yeast to humans, and has been shown in human to bind directly to DNA primase.


Pssm-ID: 399129  Cd Length: 105  Bit Score: 40.56  E-value: 4.97e-05
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 254553280   20 QLPAFNEDGL-RQVLEEMKALYEQNQSDVNEAKSAGR----GDLIP---TVKFRHCALLRN---RRCTIAYLYDRLLRIR 88
Cdd:pfam05916   2 ELPLWLAELLkRRGLVEIEPPEWLSIEELEAILADETkndlSLLPPyfyELAVLLLELERDdelKRLLRDYLRIRLAKIR 81

                          90       100
                  ....*....|....*....|...
gi 254553280   89 ALRWEYGSVL-PNSLRFHMSAEE 110
Cdd:pfam05916  82 KLAWHLNGSLsPSDLLDNLSEEE 104
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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