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Conserved domains on  [gi|145332006|ref|NP_001078125|]
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Ribosomal protein L10 family protein [Arabidopsis thaliana]

Protein Classification

uL10 family ribosomal protein( domain architecture ID 2031)

uL10 family ribosomal protein forms a tight complex with multiple copies of the small acidic protein; the complex forms a stalk structure on the large subunit of the ribosome

Gene Ontology:  GO:0070180|GO:0003735|GO:0002181
PubMed:  24524803

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
Ribosomal_L10_P0 super family cl00376
Ribosomal protein L10 family; composed of the large subunit ribosomal protein called L10 in ...
 1-244 7.33e-106

Ribosomal protein L10 family; composed of the large subunit ribosomal protein called L10 in bacteria, P0 in eukaryotes, and L10e in archaea, as well as uncharacterized P0-like eukaryotic proteins. In all three kingdoms, L10 forms a tight complex with multiple copies of the small acidic protein L12(e). This complex forms a stalk structure on the large subunit of the ribosome. The N-terminal domain (NTD) of L10 interacts with L11 protein and forms the base of the L7/L12 stalk, while the extended C-terminal helix binds to two or three dimers of the NTD of L7/L12 (L7 and L12 are identical except for an acetylated N-terminus). The L7/L12 stalk is known to contain the binding site for elongation factors G and Tu (EF-G and EF-Tu, respectively); however, there is disagreement as to whether or not L10 is involved in forming the binding site. The stalk is believed to be associated with GTPase activities in protein synthesis. In a neuroblastoma cell line, L10 has been shown to interact with the SH3 domain of Src and to activate the binding of the Nck1 adaptor protein with skeletal proteins such as the Wiskott-Aldrich Syndrome Protein (WASP) and the WASP-interacting protein (WIP). Some eukaryotic P0 sequences have an additional C-terminal domain homologous with acidic proteins P1 and P2.


The actual alignment was detected with superfamily member PTZ00135:

Pssm-ID: 469747 [Multi-domain]  Cd Length: 310  Bit Score: 310.03  E-value: 7.33e-106
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 145332006   1 MVKATKAEKKIAYDTKLCQLIDEYTQILVRSV---------RIHSENTGNTAIL----------------------NLLP 49
Cdd:PTZ00135   1 MAKPEKKAKKKAYFEKLYELLEKYKKILIVSVdnvgskqmqDIRRSLRGKAELLmgkntlirkalkqrleelpeleKLLP 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 145332006  50 LLQGNVGLIFTKGDLKEVSEEVAKYKVGAPARVGLVAPIDVVVQPGNTGLDPSQTSFFQVLNIPTKINKGTVEIITPVEL 129
Cdd:PTZ00135  81 HVKGNVGFVFTKDDLFEVKPVILENKVPAPARAGVIAPIDVVIPAGPTGMDPSQTSFFQALGIATKIVKGQIEITNEVHL 160

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 145332006 130 IKQGDKVGSSEAALLAKLGIRPFSYGLVVQSVYDNGSVFSPEVLDLTEDQLVEKFASGISMVTSLALAVSYPTLAAAPHM 209
Cdd:PTZ00135 161 IKEGQKVGASQAVLLQKLNIKPFSYGLEVLSIYDNGSIYDAKVLDITDEDIVAKFQEGVQNVAAISLAAGYPTEASAPHS 240

                        250       260       270
                 ....*....|....*....|....*....|....*
gi 145332006 210 FINAYKNALAIAVATEYTFPQAEKVKEYLKDPSKF 244
Cdd:PTZ00135 241 ILNAFKNLAAIGLESGFTFPLAEKIKEALANPSAA 275
 
Name Accession Description Interval E-value
PTZ00135 PTZ00135
60S acidic ribosomal protein P0; Provisional
 1-244 7.33e-106

60S acidic ribosomal protein P0; Provisional


Pssm-ID: 240285 [Multi-domain]  Cd Length: 310  Bit Score: 310.03  E-value: 7.33e-106
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 145332006   1 MVKATKAEKKIAYDTKLCQLIDEYTQILVRSV---------RIHSENTGNTAIL----------------------NLLP 49
Cdd:PTZ00135   1 MAKPEKKAKKKAYFEKLYELLEKYKKILIVSVdnvgskqmqDIRRSLRGKAELLmgkntlirkalkqrleelpeleKLLP 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 145332006  50 LLQGNVGLIFTKGDLKEVSEEVAKYKVGAPARVGLVAPIDVVVQPGNTGLDPSQTSFFQVLNIPTKINKGTVEIITPVEL 129
Cdd:PTZ00135  81 HVKGNVGFVFTKDDLFEVKPVILENKVPAPARAGVIAPIDVVIPAGPTGMDPSQTSFFQALGIATKIVKGQIEITNEVHL 160

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 145332006 130 IKQGDKVGSSEAALLAKLGIRPFSYGLVVQSVYDNGSVFSPEVLDLTEDQLVEKFASGISMVTSLALAVSYPTLAAAPHM 209
Cdd:PTZ00135 161 IKEGQKVGASQAVLLQKLNIKPFSYGLEVLSIYDNGSIYDAKVLDITDEDIVAKFQEGVQNVAAISLAAGYPTEASAPHS 240

                        250       260       270
                 ....*....|....*....|....*....|....*
gi 145332006 210 FINAYKNALAIAVATEYTFPQAEKVKEYLKDPSKF 244
Cdd:PTZ00135 241 ILNAFKNLAAIGLESGFTFPLAEKIKEALANPSAA 275
Ribosomal_P0_L10e cd05795
Ribosomal protein L10 family, P0 and L10e subfamily; composed of eukaryotic 60S ribosomal ...
 8-151 6.45e-58

Ribosomal protein L10 family, P0 and L10e subfamily; composed of eukaryotic 60S ribosomal protein P0 and the archaeal P0 homolog, L10e. P0 or L10e forms a tight complex with multiple copies of the small acidic protein L12(e). This complex forms a stalk structure on the large subunit of the ribosome. The stalk is known to contain the binding site for elongation factors G and Tu (EF-G and EF-Tu, respectively); however, there is disagreement as to whether or not L10 is involved in forming the binding site. The stalk is believed to be associated with GTPase activities in protein synthesis. In a neuroblastoma cell line, L10 has been shown to interact with the SH3 domain of Src and to activate the binding of the Nck1 adaptor protein with skeletal proteins such as the Wiskott-Aldrich Syndrome Protein (WASP) and the WASP-interacting protein (WIP). These eukaryotic and archaeal P0 sequences have an additional C-terminal domain homologous with acidic proteins P1 and P2.


Pssm-ID: 240221 [Multi-domain]  Cd Length: 175  Bit Score: 183.16  E-value: 6.45e-58
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 145332006   8 EKKIAYDTKLCQLIDEYTQIL------VRSVRIH--------------SENT-----------GNTAILNLLPLLQGNVG 56
Cdd:cd05795    1 EWKKEYVEKLTELLKSYPKVLivdadnVGSKQLQkirrslrgkaeilmGKNTlirralrnlgdENPELEKLLPYLKGNVG 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 145332006  57 LIFTKGDLKEVSEEVAKYKVGAPARVGLVAPIDVVVQPGNTGLDPSQTSFFQVLNIPTKINKGTVEIITPVELIKQGDKV 136
Cdd:cd05795   81 FIFTNGDPFEIRKILEENKVPAPAKPGAIAPCDVVVPAGPTGMPPGPTSFFQALGIPTKIEKGKIEIISDVVVVKKGEKV 160

                        170
                 ....*....|....*
gi 145332006 137 GSSEAALLAKLGIRP 151
Cdd:cd05795  161 GASEATLLNKLNIKP 175
RL10P_insert pfam17777
Insertion domain in 60S ribosomal protein L10P; This domain is found in prokaryotic and ...
 80-149 6.25e-22

Insertion domain in 60S ribosomal protein L10P; This domain is found in prokaryotic and archaeal ribosomal L10 protein.


Pssm-ID: 465500 [Multi-domain]  Cd Length: 71  Bit Score: 86.88  E-value: 6.25e-22
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 145332006   80 ARVGLVAPIDVVVQPGNTGLDPSQT-SFFQVLNIPTKINKGTVEIITPVELIKQGDKVGSSEAALLAKLGI 149
Cdd:pfam17777   1 ARAGAIATEDVVLPAGPTGLAPGPIePQLRALGIPTKIKKGKIEITKDYTVCKEGEKLTPEQANLLKLLGI 71
 
Name Accession Description Interval E-value
PTZ00135 PTZ00135
60S acidic ribosomal protein P0; Provisional
 1-244 7.33e-106

60S acidic ribosomal protein P0; Provisional


Pssm-ID: 240285 [Multi-domain]  Cd Length: 310  Bit Score: 310.03  E-value: 7.33e-106
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 145332006   1 MVKATKAEKKIAYDTKLCQLIDEYTQILVRSV---------RIHSENTGNTAIL----------------------NLLP 49
Cdd:PTZ00135   1 MAKPEKKAKKKAYFEKLYELLEKYKKILIVSVdnvgskqmqDIRRSLRGKAELLmgkntlirkalkqrleelpeleKLLP 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 145332006  50 LLQGNVGLIFTKGDLKEVSEEVAKYKVGAPARVGLVAPIDVVVQPGNTGLDPSQTSFFQVLNIPTKINKGTVEIITPVEL 129
Cdd:PTZ00135  81 HVKGNVGFVFTKDDLFEVKPVILENKVPAPARAGVIAPIDVVIPAGPTGMDPSQTSFFQALGIATKIVKGQIEITNEVHL 160

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 145332006 130 IKQGDKVGSSEAALLAKLGIRPFSYGLVVQSVYDNGSVFSPEVLDLTEDQLVEKFASGISMVTSLALAVSYPTLAAAPHM 209
Cdd:PTZ00135 161 IKEGQKVGASQAVLLQKLNIKPFSYGLEVLSIYDNGSIYDAKVLDITDEDIVAKFQEGVQNVAAISLAAGYPTEASAPHS 240

                        250       260       270
                 ....*....|....*....|....*....|....*
gi 145332006 210 FINAYKNALAIAVATEYTFPQAEKVKEYLKDPSKF 244
Cdd:PTZ00135 241 ILNAFKNLAAIGLESGFTFPLAEKIKEALANPSAA 275
PTZ00240 PTZ00240
60S ribosomal protein P0; Provisional
 50-230 7.78e-61

60S ribosomal protein P0; Provisional


Pssm-ID: 140267 [Multi-domain]  Cd Length: 323  Bit Score: 195.57  E-value: 7.78e-61
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 145332006  50 LLQGNVGLIFTKGDLKEVSEEVAKYKVGAPARVGLVAPIDVVVQPGNTGLDPSQTSFFQVLNIPTKINKGTVEIITPVEL 129
Cdd:PTZ00240  88 LLSGNTGLIFTNNEVQEITSVLDSHRVKAPARVGAIAPCDVIVPAGSTGMEPTQTSFFQALNIATKIAKGMVEIVTEKKV 167

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 145332006 130 IKQGDKVGSSEAALLAKLGIRPFSYGLVVQSVYDNGSVFSPEVLDLTEDqLVEKF-ASGISMVTSLALAVSYPTLAAAPH 208
Cdd:PTZ00240 168 LSVGDKVDNSTATLLQKLNISPFYYQVEVLSVWDRGVLFTREDLSMTED-VVEKMlMEGLSNVAAMSLGAGIPTAATIGP 246

                        170       180
                 ....*....|....*....|..
gi 145332006 209 MFINAYKNALAIAVATEYTFPQ 230
Cdd:PTZ00240 247 MLVDAFKNLLAVSVATSYEFEE 268
Ribosomal_P0_L10e cd05795
Ribosomal protein L10 family, P0 and L10e subfamily; composed of eukaryotic 60S ribosomal ...
 8-151 6.45e-58

Ribosomal protein L10 family, P0 and L10e subfamily; composed of eukaryotic 60S ribosomal protein P0 and the archaeal P0 homolog, L10e. P0 or L10e forms a tight complex with multiple copies of the small acidic protein L12(e). This complex forms a stalk structure on the large subunit of the ribosome. The stalk is known to contain the binding site for elongation factors G and Tu (EF-G and EF-Tu, respectively); however, there is disagreement as to whether or not L10 is involved in forming the binding site. The stalk is believed to be associated with GTPase activities in protein synthesis. In a neuroblastoma cell line, L10 has been shown to interact with the SH3 domain of Src and to activate the binding of the Nck1 adaptor protein with skeletal proteins such as the Wiskott-Aldrich Syndrome Protein (WASP) and the WASP-interacting protein (WIP). These eukaryotic and archaeal P0 sequences have an additional C-terminal domain homologous with acidic proteins P1 and P2.


Pssm-ID: 240221 [Multi-domain]  Cd Length: 175  Bit Score: 183.16  E-value: 6.45e-58
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 145332006   8 EKKIAYDTKLCQLIDEYTQIL------VRSVRIH--------------SENT-----------GNTAILNLLPLLQGNVG 56
Cdd:cd05795    1 EWKKEYVEKLTELLKSYPKVLivdadnVGSKQLQkirrslrgkaeilmGKNTlirralrnlgdENPELEKLLPYLKGNVG 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 145332006  57 LIFTKGDLKEVSEEVAKYKVGAPARVGLVAPIDVVVQPGNTGLDPSQTSFFQVLNIPTKINKGTVEIITPVELIKQGDKV 136
Cdd:cd05795   81 FIFTNGDPFEIRKILEENKVPAPAKPGAIAPCDVVVPAGPTGMPPGPTSFFQALGIPTKIEKGKIEIISDVVVVKKGEKV 160

                        170
                 ....*....|....*
gi 145332006 137 GSSEAALLAKLGIRP 151
Cdd:cd05795  161 GASEATLLNKLNIKP 175
rplP0 PRK04019
acidic ribosomal protein P0; Validated
 37-226 2.12e-36

acidic ribosomal protein P0; Validated


Pssm-ID: 179712 [Multi-domain]  Cd Length: 330  Bit Score: 132.30  E-value: 2.12e-36
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 145332006  37 ENTGNTAILNLLPLLQGNVGLIFTKGDLKEVSEEVAKYKVGAPARVGLVAPIDVVVQPGNTGLDPSQ-TSFFQVLNIPTK 115
Cdd:PRK04019  64 EEAGEEDLEKLEDYLEGQVALIFTNMNPFKLYKLLEKSKTPAPAKPGDIAPEDIVVPAGPTGFPPGPiLSELQKLGIPAR 143

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 145332006 116 INKGTVEIITPVELIKQGDKVGSSEAALLAKLGIRPFSYGLVVQSVYDNGSVFSPEVLDLTEDQLVEKFASGISMVTSLA 195
Cdd:PRK04019 144 IQKGKIVIKKDTVVAKAGEVISPELANVLQKLGIKPIEVGLDLKAAYEDGVIYTPEVLAIDEEKYRSDIQEAAQNAFNLA 223

                        170       180       190
                 ....*....|....*....|....*....|.
gi 145332006 196 LAVSYPTLAAAPHMFINAYKNALAIAVATEY 226
Cdd:PRK04019 224 VNAAYPTPETLETLIQKAFREAKALAVEAGI 254
RL10P_insert pfam17777
Insertion domain in 60S ribosomal protein L10P; This domain is found in prokaryotic and ...
 80-149 6.25e-22

Insertion domain in 60S ribosomal protein L10P; This domain is found in prokaryotic and archaeal ribosomal L10 protein.


Pssm-ID: 465500 [Multi-domain]  Cd Length: 71  Bit Score: 86.88  E-value: 6.25e-22
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 145332006   80 ARVGLVAPIDVVVQPGNTGLDPSQT-SFFQVLNIPTKINKGTVEIITPVELIKQGDKVGSSEAALLAKLGI 149
Cdd:pfam17777   1 ARAGAIATEDVVLPAGPTGLAPGPIePQLRALGIPTKIKKGKIEITKDYTVCKEGEKLTPEQANLLKLLGI 71
Ribosomal_P0_like cd05796
Ribosomal protein L10 family, P0-like protein subfamily; composed of uncharacterized ...
 47-136 2.23e-11

Ribosomal protein L10 family, P0-like protein subfamily; composed of uncharacterized eukaryotic proteins with similarity to the 60S ribosomal protein P0, including the Saccharomyces cerevisiae protein called mRNA turnover protein 4 (MRT4). MRT4 may be involved in mRNA decay. P0 forms a tight complex with multiple copies of the small acidic protein L12(e). This complex forms a stalk structure on the large subunit of the ribosome. It occupies the L7/L12 stalk of the ribosome. The stalk is known to contain the binding site for elongation factors EF-G and EF-Tu; however, there is disagreement as to whether or not P0 is involved in forming the binding site. The stalk is believed to be associated with GTPase activities in protein synthesis. In a neuroblastoma cell line, P0 has been shown to interact with the SH3 domain of Src and to activate the binding of the Nck1 adaptor protein with skeletal proteins such as the Wiskott-Aldrich Syndrome Protein (WASP) and the WASP-interacting protein (WIP). Some eukaryotic P0 sequences have an additional C-terminal domain homologous with acidic proteins P1 and P2.


Pssm-ID: 240222 [Multi-domain]  Cd Length: 163  Bit Score: 61.05  E-value: 2.23e-11
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 145332006  47 LLPLLQGNVGLIFTKGDLKEVSEEVAKYKVGAPARVGLVAPIDVVVQPGNtgLDP---SQTSFFQVLNIPTKINKGTVEI 123
Cdd:cd05796   72 LSKYLKGQVGLLFTNEPPEEVIEYFDSYSEPDFARAGSIATETVTLPEGP--LEQfphSMEPQLRKLGLPTKLKKGVITL 149

                         90
                 ....*....|...
gi 145332006 124 ITPVELIKQGDKV 136
Cdd:cd05796  150 EADYVVCEEGKVL 162
Ribosomal_60s pfam00428
60s Acidic ribosomal protein; This family includes archaebacterial L12, eukaryotic P0, P1 and ...
 200-266 1.52e-03

60s Acidic ribosomal protein; This family includes archaebacterial L12, eukaryotic P0, P1 and P2.


Pssm-ID: 459808  Cd Length: 87  Bit Score: 36.85  E-value: 1.52e-03
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 145332006  200 YPTLAAAPHMFINAYKNALAIAVATEYTFPQAEKVKEYLKDPSKFAVASVAAVSADAGGGAPAAAKV 266
Cdd:pfam00428   1 EPTAANIKKVLKAAGVNVEAVRVKLFAKALEGKNIKELLANGGAKAAAAAAAAAAAAAAAAAAAAAE 67
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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