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Conserved domains on  [gi|145328734|ref|NP_001077902|]
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Iron-sulfur cluster biosynthesis family protein [Arabidopsis thaliana]

Protein Classification

iron-sulfur cluster assembly accessory protein( domain architecture ID 1258)

iron-sulfur cluster assembly accessory protein from the HesB/IscA family is involved in the assembly of iron-sulfur proteins

CATH:  2.60.300.12
Gene Ontology:  GO:0005506|GO:0016226
SCOP:  4003950

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
Fe-S_biosyn super family cl00400
Iron-sulphur cluster biosynthesis; This family is involved in iron-sulphur cluster ...
 24-104 1.73e-31

Iron-sulphur cluster biosynthesis; This family is involved in iron-sulphur cluster biosynthesis. Its members include proteins that are involved in nitrogen fixation such as the HesB and HesB-like proteins.


The actual alignment was detected with superfamily member TIGR00049:

Pssm-ID: 444888 [Multi-domain]  Cd Length: 105  Bit Score: 106.90  E-value: 1.73e-31
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 145328734   24 TLTDEAASRVHHLLQQRQKP--FLRLGVKARGCNGLSYTLNYADEKGKFDELVEEKGVRILVEPKALMHVIGTKMDFVDD 101
Cdd:TIGR00049   1 TLTDSAAKRIKALLAGEGEPnlGLRVGVKGGGCSGLQYGLEFDDEPNEDDEVFEQDGVKVVVDPKSLPYLDGSEIDYVEE 80


                  ...
gi 145328734  102 KLR 104
Cdd:TIGR00049  81 LLG 83
 
Name Accession Description Interval E-value
TIGR00049 TIGR00049
Iron-sulfur cluster assembly accessory protein; Proteins in this subfamily appear to be ...
 24-104 1.73e-31

Iron-sulfur cluster assembly accessory protein; Proteins in this subfamily appear to be associated with the process of FeS-cluster assembly. The HesB proteins are associated with the nif gene cluster and the Rhizobium gene IscN has been shown to be required for nitrogen fixation. Nitrogenase includes multiple FeS clusters and many genes for their assembly. The E. coli SufA protein is associated with SufS, a NifS homolog and SufD which are involved in the FeS cluster assembly of the FhnF protein. The Azotobacter protein IscA (homologs of which are also found in E.coli) is associated which IscS, another NifS homolog and IscU, a nifU homolog as well as other factors consistent with a role in FeS cluster chemistry. A homolog from Geobacter contains a selenocysteine in place of an otherwise invariant cysteine, further suggesting a role in redox chemistry. [Biosynthesis of cofactors, prosthetic groups, and carriers, Other]


Pssm-ID: 272875 [Multi-domain]  Cd Length: 105  Bit Score: 106.90  E-value: 1.73e-31
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 145328734   24 TLTDEAASRVHHLLQQRQKP--FLRLGVKARGCNGLSYTLNYADEKGKFDELVEEKGVRILVEPKALMHVIGTKMDFVDD 101
Cdd:TIGR00049   1 TLTDSAAKRIKALLAGEGEPnlGLRVGVKGGGCSGLQYGLEFDDEPNEDDEVFEQDGVKVVVDPKSLPYLDGSEIDYVEE 80


                  ...
gi 145328734  102 KLR 104
Cdd:TIGR00049  81 LLG 83
IscA COG0316
Fe-S cluster assembly iron-binding protein IscA [Posttranslational modification, protein ...
 22-103 3.33e-31

Fe-S cluster assembly iron-binding protein IscA [Posttranslational modification, protein turnover, chaperones];


Pssm-ID: 440085 [Multi-domain]  Cd Length: 107  Bit Score: 106.00  E-value: 3.33e-31
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 145328734  22 VLTLTDEAASRVHHLLQQRQKP--FLRLGVKARGCNGLSYTLNYADEKGKFDELVEEKGVRILVEPKALMHVIGTKMDFV 99
Cdd:COG0316    1 PITLTDAAAKRIKRLLAKEGNPglGLRVGVKGGGCSGFSYGLDFDDEPNEDDLVFEQDGVKVVVDPKSLPYLDGTEIDYV 80


                 ....
gi 145328734 100 DDKL 103
Cdd:COG0316   81 EELL 84
iscA PRK09502
iron-sulfur cluster assembly protein IscA;
23-103 4.07e-20

iron-sulfur cluster assembly protein IscA;


Pssm-ID: 181914 [Multi-domain]  Cd Length: 107  Bit Score: 77.99  E-value: 4.07e-20
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 145328734  23 LTLTDEAASRVHHLLQQRQKPF-LRLGVKARGCNGLSYTLNYADEKGKFDELVEEKGVRILVEPKALMHVIGTKMDFVDD 101
Cdd:PRK09502   3 ITLSDSAAARVNTFLANRGKGFgLRLGVRTSGCSGMAYVLEFVDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKE 82


                 ..
gi 145328734 102 KL 103
Cdd:PRK09502  83 GL 84
Fe-S_biosyn pfam01521
Iron-sulphur cluster biosynthesis; This family is involved in iron-sulphur cluster ...
 23-103 4.70e-09

Iron-sulphur cluster biosynthesis; This family is involved in iron-sulphur cluster biosynthesis. Its members include proteins that are involved in nitrogen fixation such as the HesB and HesB-like proteins.


Pssm-ID: 426304  Cd Length: 111  Bit Score: 49.57  E-value: 4.70e-09
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 145328734   23 LTLTDEAASRVHHLLQQRQKPfLRLGVK------ARGC--NGLSYTLNYADEK-GKFDELVEEKGVRILVEPKALMHV-I 92
Cdd:pfam01521   3 ITITDAAAERLKKLLAGDKKE-LRLDVDdgggpySKGGcsIGGKFSLVLVDEPdPDYDEVIESNGGPIYVDSYSLPFLdE 81

                          90
                  ....*....|.
gi 145328734   93 GTKMDFVDDKL 103
Cdd:pfam01521  82 GLTLDFVEDLG 92
 
Name Accession Description Interval E-value
TIGR00049 TIGR00049
Iron-sulfur cluster assembly accessory protein; Proteins in this subfamily appear to be ...
 24-104 1.73e-31

Iron-sulfur cluster assembly accessory protein; Proteins in this subfamily appear to be associated with the process of FeS-cluster assembly. The HesB proteins are associated with the nif gene cluster and the Rhizobium gene IscN has been shown to be required for nitrogen fixation. Nitrogenase includes multiple FeS clusters and many genes for their assembly. The E. coli SufA protein is associated with SufS, a NifS homolog and SufD which are involved in the FeS cluster assembly of the FhnF protein. The Azotobacter protein IscA (homologs of which are also found in E.coli) is associated which IscS, another NifS homolog and IscU, a nifU homolog as well as other factors consistent with a role in FeS cluster chemistry. A homolog from Geobacter contains a selenocysteine in place of an otherwise invariant cysteine, further suggesting a role in redox chemistry. [Biosynthesis of cofactors, prosthetic groups, and carriers, Other]


Pssm-ID: 272875 [Multi-domain]  Cd Length: 105  Bit Score: 106.90  E-value: 1.73e-31
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 145328734   24 TLTDEAASRVHHLLQQRQKP--FLRLGVKARGCNGLSYTLNYADEKGKFDELVEEKGVRILVEPKALMHVIGTKMDFVDD 101
Cdd:TIGR00049   1 TLTDSAAKRIKALLAGEGEPnlGLRVGVKGGGCSGLQYGLEFDDEPNEDDEVFEQDGVKVVVDPKSLPYLDGSEIDYVEE 80


                  ...
gi 145328734  102 KLR 104
Cdd:TIGR00049  81 LLG 83
IscA COG0316
Fe-S cluster assembly iron-binding protein IscA [Posttranslational modification, protein ...
 22-103 3.33e-31

Fe-S cluster assembly iron-binding protein IscA [Posttranslational modification, protein turnover, chaperones];


Pssm-ID: 440085 [Multi-domain]  Cd Length: 107  Bit Score: 106.00  E-value: 3.33e-31
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 145328734  22 VLTLTDEAASRVHHLLQQRQKP--FLRLGVKARGCNGLSYTLNYADEKGKFDELVEEKGVRILVEPKALMHVIGTKMDFV 99
Cdd:COG0316    1 PITLTDAAAKRIKRLLAKEGNPglGLRVGVKGGGCSGFSYGLDFDDEPNEDDLVFEQDGVKVVVDPKSLPYLDGTEIDYV 80


                 ....
gi 145328734 100 DDKL 103
Cdd:COG0316   81 EELL 84
iscA PRK09502
iron-sulfur cluster assembly protein IscA;
23-103 4.07e-20

iron-sulfur cluster assembly protein IscA;


Pssm-ID: 181914 [Multi-domain]  Cd Length: 107  Bit Score: 77.99  E-value: 4.07e-20
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 145328734  23 LTLTDEAASRVHHLLQQRQKPF-LRLGVKARGCNGLSYTLNYADEKGKFDELVEEKGVRILVEPKALMHVIGTKMDFVDD 101
Cdd:PRK09502   3 ITLSDSAAARVNTFLANRGKGFgLRLGVRTSGCSGMAYVLEFVDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKE 82


                 ..
gi 145328734 102 KL 103
Cdd:PRK09502  83 GL 84
sufA PRK09504
iron-sulfur cluster assembly scaffold protein; Provisional
 21-103 3.04e-15

iron-sulfur cluster assembly scaffold protein; Provisional


Pssm-ID: 181915  Cd Length: 122  Bit Score: 65.91  E-value: 3.04e-15
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 145328734  21 QVLTLTDEAASRVHHLLQQR-QKPFLRLGVKARGCNGLSYTLNYADEKGKFDELVEEKGVRILVEPKALMHVIGTKMDFV 99
Cdd:PRK09504  16 QGLTLTPAAAAHIRELMAKQpGMKGVRLGVKQTGCAGFGYVLDSVSEPDKDDLVFEHDGAKLFVPLQAMPFIDGTEVDYV 95


                 ....
gi 145328734 100 DDKL 103
Cdd:PRK09504  96 REGL 99
Fe-S_biosyn pfam01521
Iron-sulphur cluster biosynthesis; This family is involved in iron-sulphur cluster ...
 23-103 4.70e-09

Iron-sulphur cluster biosynthesis; This family is involved in iron-sulphur cluster biosynthesis. Its members include proteins that are involved in nitrogen fixation such as the HesB and HesB-like proteins.


Pssm-ID: 426304  Cd Length: 111  Bit Score: 49.57  E-value: 4.70e-09
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 145328734   23 LTLTDEAASRVHHLLQQRQKPfLRLGVK------ARGC--NGLSYTLNYADEK-GKFDELVEEKGVRILVEPKALMHV-I 92
Cdd:pfam01521   3 ITITDAAAERLKKLLAGDKKE-LRLDVDdgggpySKGGcsIGGKFSLVLVDEPdPDYDEVIESNGGPIYVDSYSLPFLdE 81

                          90
                  ....*....|.
gi 145328734   93 GTKMDFVDDKL 103
Cdd:pfam01521  82 GLTLDFVEDLG 92
PLN03082 PLN03082
Iron-sulfur cluster assembly; Provisional
 21-104 6.18e-08

Iron-sulfur cluster assembly; Provisional


Pssm-ID: 215564  Cd Length: 163  Bit Score: 48.00  E-value: 6.18e-08
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 145328734  21 QVLTLTDEAASRVHHLLQQR---QKPFLRLGVKARGCNGLSYTLNYADEKGKFDELVEEKGVRILVEPKALMHVIGTKMD 97
Cdd:PLN03082  53 DAVHMTDNCIRRLKELQTSEpsaEDKMLRLSVETGGCSGFQYVFELDDKTNSDDRVFEKDGVKLVVDNISYDFVKGATVD 132


                 ....*..
gi 145328734  98 FVDDKLR 104
Cdd:PLN03082 133 YVEELIR 139
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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