NCBI Home Page NCBI Site Search page NCBI Guide that lists and describes the NCBI resources
Conserved domains on  [gi|145326108|ref|NP_001077763|]
View 

Major Facilitator Superfamily with SPX (SYG1/Pho81/XPR1) domain-containing protein [Arabidopsis thaliana]

Protein Classification

SPX domain-containing membrane protein( domain architecture ID 12997668)

SPX domain-containing membrane protein with a major facilitator superfamily (MFS) transporter domain may function in phosphate homeostasis and in the transport across cytoplasmic or internal membranes various substrates including sugar phosphates

CATH:  1.20.1250.20
Gene Ontology:  GO:0022857|GO:0055085|GO:0016020
PubMed:  22403821|26098515|26758938|26234418
SCOP:  3000310
TCDB:  2.A.1

Graphical summary

 Zoom to residue level

show extra options »

Show site features     Horizontal zoom: ×

List of domain hits

 Name Accession Description Interval E-value
SPX-MFS_plant cd14479
SPX domain of proteins found in plants and stramenopiles; most have a C-terminal MFS domain; ...
 2-141 1.03e-84

SPX domain of proteins found in plants and stramenopiles; most have a C-terminal MFS domain; This region has been named the SPX domain after (Syg1, Pho81 and XPR1). The SPX domain is found at the amino terminus of a variety of proteins. This family, mostly found in plants, contains a C-terminal MFS domain (major facilitator superfamily), suggesting a function as a secondary transporter. The function of this N-terminal region is unclear, although it might be involved in regulating transport.


:

Pssm-ID: 269900 [Multi-domain]  Cd Length: 140  Bit Score: 262.99  E-value: 1.03e-84
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 145326108   2 VAFGKYLQRKQIEEWSGYYINYKLMKKKVKQYAEQIQGGSQHPRHVLKDFSRMLDTQIETTVLFMLEQQGLLSGRLAKLR 81
Cdd:cd14479    1 VNFGKKLKEDQIPEWEGYYINYKLLKKKVKQYVQQTQDGGQDRRDVLKDFSKLLDDQIEKIVLFLLEQQGLLASRLEKLG 80

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|
gi 145326108  82 ESHDAILEQPDISRIFELREAYRDVGRDLLQLLKFVELNAIGLRKILKKFDKRFGYRFAD 141
Cdd:cd14479   81 EQREALQEQPDLSQISELREAYRAVGLDLLKLLKFVELNATGLRKILKKFDKRFGYRFTD 140
MFS super family cl28910
Major Facilitator Superfamily; The Major Facilitator Superfamily (MFS) is a large and diverse ...
 249-657 7.05e-23

Major Facilitator Superfamily; The Major Facilitator Superfamily (MFS) is a large and diverse group of secondary transporters that includes uniporters, symporters, and antiporters. MFS proteins facilitate the transport across cytoplasmic or internal membranes of a variety of substrates including ions, sugar phosphates, drugs, neurotransmitters, nucleosides, amino acids, and peptides. They do so using the electrochemical potential of the transported substrates. Uniporters transport a single substrate, while symporters and antiporters transport two substrates in the same or in opposite directions, respectively, across membranes. MFS proteins are typically 400 to 600 amino acids in length, and the majority contain 12 transmembrane alpha helices (TMs) connected by hydrophilic loops. The N- and C-terminal halves of these proteins display weak similarity and may be the result of a gene duplication/fusion event. Based on kinetic studies and the structures of a few bacterial superfamily members, GlpT (glycerol-3-phosphate transporter), LacY (lactose permease), and EmrD (multidrug transporter), MFS proteins are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement. Bacterial members function primarily for nutrient uptake, and as drug-efflux pumps to confer antibiotic resistance. Some MFS proteins have medical significance in humans such as the glucose transporter Glut4, which is impaired in type II diabetes, and glucose-6-phosphate transporter (G6PT), which causes glycogen storage disease when mutated.


The actual alignment was detected with superfamily member cd17326:

Pssm-ID: 475125 [Multi-domain]  Cd Length: 374  Bit Score: 101.13  E-value: 7.05e-23
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 145326108 249 SLLLNLGNTFLYMvntyIIVPTADDYSmsLGAAATVCGVVIGSMAVAQVFSSVYFSAWSNK--SYFKPLVFSSIALFIGN 326
Cdd:cd17326    8 MFLSSLQFSIVLT----SIWPYLQKID--PTATASFFGWVVAAYSLGQLVFSPVFGYWSNKtsSVKEPLIVSILIAIVGN 81

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 145326108 327 LMYALAYDA--NSIALLLLGRVCCGLG-SARAVNRRYISDCVPLRIRMQASAGFVSASALGMACGPALAGLL-----QIK 398
Cdd:cd17326   82 ILYALAEYFpsNRKYVMLVARFLIGVGaGNVAVLRAYVAAASTLKDRTRAMAVISAAQALGFIVGPALQAAFtpigyPGY 161

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 145326108 399 FKFYKFTFNQSTLPGWVMAVAWLFYLVWLCISFREplrdtedgeknnrnettsatdrvessrveeglrlpllitsgikpe 478
Cdd:cd17326  162 VIGIKLRFNMYTAPAWLMAILGIINIILILFFFKE--------------------------------------------- 196

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 145326108 479 deeecdeseespedshkpansfieayrlltpsvkVQLLIYFMLKYSMEILL-SESSVITSYYFSWT----TSSVAIFLAC 553
Cdd:cd17326  197 ----------------------------------VVCIFLFFVVLFIFANLeTIGTPLTMDMYGWTreeaVLYNGIILAV 242

                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 145326108 554 LGLTVLPINILVGSYISNMFEDRQILLTSEIIVFLGILFsfnlfvpyTVPQYVISGLIMFVAAEVLEGVNLSLLSRVMSS 633
Cdd:cd17326  243 SGVLSVLVFLLFKFLSLGLIDERKILLGGLILFFLGFVV--------TLPWGITGVILFGLGYPIGNVMLYTLYSKILGP 314

                        410       420
                 ....*....|....*....|....
gi 145326108 634 RlSKGTYNGGLlsTEAGTLARVVA 657
Cdd:cd17326  315 K-PQGTMQGVL--TASGSLARTLG 335
 
Name Accession Description Interval E-value
SPX-MFS_plant cd14479
SPX domain of proteins found in plants and stramenopiles; most have a C-terminal MFS domain; ...
 2-141 1.03e-84

SPX domain of proteins found in plants and stramenopiles; most have a C-terminal MFS domain; This region has been named the SPX domain after (Syg1, Pho81 and XPR1). The SPX domain is found at the amino terminus of a variety of proteins. This family, mostly found in plants, contains a C-terminal MFS domain (major facilitator superfamily), suggesting a function as a secondary transporter. The function of this N-terminal region is unclear, although it might be involved in regulating transport.


Pssm-ID: 269900 [Multi-domain]  Cd Length: 140  Bit Score: 262.99  E-value: 1.03e-84
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 145326108   2 VAFGKYLQRKQIEEWSGYYINYKLMKKKVKQYAEQIQGGSQHPRHVLKDFSRMLDTQIETTVLFMLEQQGLLSGRLAKLR 81
Cdd:cd14479    1 VNFGKKLKEDQIPEWEGYYINYKLLKKKVKQYVQQTQDGGQDRRDVLKDFSKLLDDQIEKIVLFLLEQQGLLASRLEKLG 80

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|
gi 145326108  82 ESHDAILEQPDISRIFELREAYRDVGRDLLQLLKFVELNAIGLRKILKKFDKRFGYRFAD 141
Cdd:cd14479   81 EQREALQEQPDLSQISELREAYRAVGLDLLKLLKFVELNATGLRKILKKFDKRFGYRFTD 140
MFS_MFSD8 cd17326
Major facilitator superfamily domain-containing protein 8; Major facilitator superfamily (MFS) ...
 249-657 7.05e-23

Major facilitator superfamily domain-containing protein 8; Major facilitator superfamily (MFS) domain-containing protein 8 (MFSD8) is also called ceroid-lipofuscinosis neuronal protein 7 (CLN7). It is a polytopic lysosomal membrane protein that may transport small solutes by using chemiosmotic ion gradients. Mutations in MFSD8/CLN7 cause a variant of late-infantile neuronal ceroid lipofuscinoses (vLINCL), a neurodegenerative lysosomal storage disorder. Some variants are associated with nonsyndromic autosomal recessive macular dystrophy. MFSD8/CLN7 belongs to the Eukaryotic Solute carrier 46 (SLC46)/Bacterial Tetracycline resistance -like (SLC46/TetA-like) family of the Major Facilitator Superfamily (MFS) of transporters. MFS proteins are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.


Pssm-ID: 340884 [Multi-domain]  Cd Length: 374  Bit Score: 101.13  E-value: 7.05e-23
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 145326108 249 SLLLNLGNTFLYMvntyIIVPTADDYSmsLGAAATVCGVVIGSMAVAQVFSSVYFSAWSNK--SYFKPLVFSSIALFIGN 326
Cdd:cd17326    8 MFLSSLQFSIVLT----SIWPYLQKID--PTATASFFGWVVAAYSLGQLVFSPVFGYWSNKtsSVKEPLIVSILIAIVGN 81

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 145326108 327 LMYALAYDA--NSIALLLLGRVCCGLG-SARAVNRRYISDCVPLRIRMQASAGFVSASALGMACGPALAGLL-----QIK 398
Cdd:cd17326   82 ILYALAEYFpsNRKYVMLVARFLIGVGaGNVAVLRAYVAAASTLKDRTRAMAVISAAQALGFIVGPALQAAFtpigyPGY 161

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 145326108 399 FKFYKFTFNQSTLPGWVMAVAWLFYLVWLCISFREplrdtedgeknnrnettsatdrvessrveeglrlpllitsgikpe 478
Cdd:cd17326  162 VIGIKLRFNMYTAPAWLMAILGIINIILILFFFKE--------------------------------------------- 196

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 145326108 479 deeecdeseespedshkpansfieayrlltpsvkVQLLIYFMLKYSMEILL-SESSVITSYYFSWT----TSSVAIFLAC 553
Cdd:cd17326  197 ----------------------------------VVCIFLFFVVLFIFANLeTIGTPLTMDMYGWTreeaVLYNGIILAV 242

                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 145326108 554 LGLTVLPINILVGSYISNMFEDRQILLTSEIIVFLGILFsfnlfvpyTVPQYVISGLIMFVAAEVLEGVNLSLLSRVMSS 633
Cdd:cd17326  243 SGVLSVLVFLLFKFLSLGLIDERKILLGGLILFFLGFVV--------TLPWGITGVILFGLGYPIGNVMLYTLYSKILGP 314

                        410       420
                 ....*....|....*....|....
gi 145326108 634 RlSKGTYNGGLlsTEAGTLARVVA 657
Cdd:cd17326  315 K-PQGTMQGVL--TASGSLARTLG 335
AraJ COG2814
Predicted arabinose efflux permease AraJ, MFS family [Carbohydrate transport and metabolism];
249-646 1.38e-13

Predicted arabinose efflux permease AraJ, MFS family [Carbohydrate transport and metabolism];


Pssm-ID: 442063 [Multi-domain]  Cd Length: 348  Bit Score: 72.70  E-value: 1.38e-13
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 145326108 249 SLLLNLGNTFLYMVNTYIIVPTADDYSMSLGAAATVCGVVIGSMAVAQVFSSVYFSAWSNKSYFKPLVFSSIALF-IGNL 327
Cdd:COG2814   11 ALLALALGAFLSGLGIGIVLPALPLIAADLGASPAQAGLVVTAYLLGAALGAPLAGRLADRFGRRRVLLLGLLLFaLGSL 90

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 145326108 328 MYALaydANSIALLLLGRVCCGLGSA--RAVNRRYISDCVPLRIRMQASAGFVSASALGMACGPALAGLLqikfkfykft 405
Cdd:COG2814   91 LCAL---APSLWLLLAARFLQGLGAGalFPAALALIADLVPPERRGRALGLLGAGLGLGPALGPLLGGLL---------- 157

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 145326108 406 fNQSTLPGWVMAVAWLFYLV-WLCISFREPlrdtedgeknnrnETTSATDRVESSRVEEGLRLPLLITSgikpedeeecd 484
Cdd:COG2814  158 -ADLFGWRWVFLVNAVLALLaLLLLLRLLP-------------ESRPAARARLRGSLRELLRRPRLLLL----------- 212

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 145326108 485 eseespedshkpansfieayrlltpsvkvqLLIYFMLKYSMEILLSESSVITSYYFSWTTSSVAIFLACLGLTVLPINIL 564
Cdd:COG2814  213 ------------------------------LLLAFLLGFGFFALFTYLPLYLQEVLGLSASAAGLLLALFGLGGVLGALL 262

                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 145326108 565 VGsYISNMFEDRQILLTSEIIVFLGILFsfnLFVPYTVPQYVISGLIMFVAAEVLEGVNLSLLSRVMSSRLsKGTYNGGL 644
Cdd:COG2814  263 AG-RLADRFGRRRLLLIGLLLLALGLLL---LALAGSLWLLLLALFLLGFGFGLLFPLLQALVAELAPPEA-RGRASGLY 337


                 ..
gi 145326108 645 LS 646
Cdd:COG2814  338 NS 339
MFS_1 pfam07690
Major Facilitator Superfamily;
257-642 6.55e-10

Major Facilitator Superfamily;


Pssm-ID: 429598 [Multi-domain]  Cd Length: 344  Bit Score: 61.28  E-value: 6.55e-10
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 145326108  257 TFLYMVNTYIIVPTADDYSM-SLGAAATVCGVVIGSMAVAQVFSSVYFSAWSNKSYFKPLVFSSIALFIgnLMYALAYDA 335
Cdd:pfam07690   5 AFLAALGRSLLGPALPLLLAeDLGISPTEIGLLLTLFSLGYALAQPLAGRLSDRFGRRRVLLIGLLLFA--LGLLLLLFA 82

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 145326108  336 NSIALLLLGRVCCGLGSA--RAVNRRYISDCVPLRIRMQASAGFVSASALGMACGPALAGLLqIKFKFYKFTFnqstlpg 413
Cdd:pfam07690  83 SSLWLLLVLRVLQGLGAGalFPAALALIADWFPPEERGRALGLVSAGFGLGAALGPLLGGLL-ASLFGWRAAF------- 154

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 145326108  414 WVMAVAWLFYLVWLCISFREPLRDTEDGEKnnrnettsatdrvessrveeglrlpllitsgikpedeeecdeseespeds 493
Cdd:pfam07690 155 LILAILSLLAAVLLLLPRPPPESKRPKPAE-------------------------------------------------- 184

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 145326108  494 hKPANSFIEAYRLLTPSVKVQLLIYFMLKYSMEILLSESSVITSYYFSWTTSSVAIFLACLGLTVLPINILVGsYISNMF 573
Cdd:pfam07690 185 -EARLSLIVAWKALLRDPVLWLLLALLLFGFAFFGLLTYLPLYQEVLGLSALLAGLLLGLGGLLGAIGRLLLG-RLSDRL 262

                         330       340       350       360       370       380
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 145326108  574 EDRQILLTSEIIVFLGILFSFNLFVPYTVPQYVISGLIMFVAAEVLEGVNLSLLSRVMSSRLSkGTYNG 642
Cdd:pfam07690 263 GRRRRLLLALLLLILAALGLLLLSLTLSSLWLLLALLLLGFGFGLVFPALNALVSDLAPKEER-GTASG 330
SPX pfam03105
SPX domain; We have named this region the SPX domain after SYG1, Pho81 and XPR1. This 180 ...
 108-147 1.70e-08

SPX domain; We have named this region the SPX domain after SYG1, Pho81 and XPR1. This 180 residue long domain is found at the amino terminus of a variety of proteins. In the yeast protein SYG1, the N-terminus directly binds to the G-protein beta subunit and inhibits transduction of the mating pheromone signal. Similarly, the N-terminus of the human XPR1 protein binds directly to the beta subunit of the G-protein heterotrimer leading to increased production of cAMP. These findings suggest that all the members of this family are involved in G-protein associated signal transduction. The N-termini of several proteins involved in the regulation of phosphate transport, including the putative phosphate level sensors PHO81 from Saccharomyces cerevisiae and NUC-2 from Neurospora crassa, are also members of this family. The SPX domain of S. cerevisiae low-affinity phosphate transporters Pho87 and Pho90 auto-regulates uptake and prevents efflux. This SPX dependent inhibition is mediated by the physical interaction with Spl2 NUC-2 contains several ankyrin repeats pfam00023. Several members of this family are annotated as XPR1 proteins: the xenotropic and polytropic retrovirus receptor confers susceptibility to infection with murine xenotropic and polytropic leukaemia viruses (MLV). Infection by these retroviruses can inhibit XPR1-mediated cAMP signalling and result in cell toxicity and death. The similarity between SYG1, phosphate regulators and XPR1 sequences has been previously noted, as has the additional similarity to several predicted proteins, of unknown function, from Drosophila melanogaster, Arabidopsis thaliana, Caenorhabditis elegans, Schizosaccharomyces pombe, and Saccharomyces cerevisiae, and many other diverse organizms. In addition, given the similarities between XPR1 and SYG1 and phosphate regulatory proteins, it has been proposed that XPR1 might be involved in G-protein associated signal transduction and may itself function as a phosphate sensor.


Pssm-ID: 460807 [Multi-domain]  Cd Length: 339  Bit Score: 56.80  E-value: 1.70e-08
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|
gi 145326108  108 RDLLQLLKFVELNAIGLRKILKKFDKRFGYRFADYYVKTR 147
Cdd:pfam03105 300 RGLELLKSYSELNRTAFRKILKKFDKVTSLNASKDYMKEV 339
COG5408 COG5408
SPX domain-containing protein [Signal transduction mechanisms];
109-152 1.13e-03

SPX domain-containing protein [Signal transduction mechanisms];


Pssm-ID: 227695 [Multi-domain]  Cd Length: 296  Bit Score: 41.74  E-value: 1.13e-03
                         10        20        30        40
                 ....*....|....*....|....*....|....*....|....
gi 145326108 109 DLLQLLKFVELNAIGLRKILKKFDKRFGYRFADYYVKtRANHPY 152
Cdd:COG5408  253 QLHQLKSFIELNYTGFSKITKKYDKTLHQNLRHEYMS-RSVNEY 295
 
Name Accession Description Interval E-value
SPX-MFS_plant cd14479
SPX domain of proteins found in plants and stramenopiles; most have a C-terminal MFS domain; ...
 2-141 1.03e-84

SPX domain of proteins found in plants and stramenopiles; most have a C-terminal MFS domain; This region has been named the SPX domain after (Syg1, Pho81 and XPR1). The SPX domain is found at the amino terminus of a variety of proteins. This family, mostly found in plants, contains a C-terminal MFS domain (major facilitator superfamily), suggesting a function as a secondary transporter. The function of this N-terminal region is unclear, although it might be involved in regulating transport.


Pssm-ID: 269900 [Multi-domain]  Cd Length: 140  Bit Score: 262.99  E-value: 1.03e-84
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 145326108   2 VAFGKYLQRKQIEEWSGYYINYKLMKKKVKQYAEQIQGGSQHPRHVLKDFSRMLDTQIETTVLFMLEQQGLLSGRLAKLR 81
Cdd:cd14479    1 VNFGKKLKEDQIPEWEGYYINYKLLKKKVKQYVQQTQDGGQDRRDVLKDFSKLLDDQIEKIVLFLLEQQGLLASRLEKLG 80

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|
gi 145326108  82 ESHDAILEQPDISRIFELREAYRDVGRDLLQLLKFVELNAIGLRKILKKFDKRFGYRFAD 141
Cdd:cd14479   81 EQREALQEQPDLSQISELREAYRAVGLDLLKLLKFVELNATGLRKILKKFDKRFGYRFTD 140
SPX cd14447
Domain found in Syg1, Pho81, XPR1, and related proteins; This region has been named the SPX ...
 4-133 2.36e-27

Domain found in Syg1, Pho81, XPR1, and related proteins; This region has been named the SPX domain after (Syg1, Pho81 and XPR1). This domain is found at the amino terminus of a variety of proteins. In the yeast protein Syg1, the N-terminus directly binds to the G-protein beta subunit and inhibits transduction of the mating pheromone signal. Similarly, the N-terminus of the human XPR1 protein binds directly to the beta subunit of the G-protein heterotrimer leading to increased production of cAMP. These findings suggest that members of this family are involved in G-protein associated signal transduction. The N-termini of several proteins involved in the regulation of phosphate transport, including the putative phosphate level sensors Pho81 from Saccharomyces cerevisiae and NUC-2 from Neurospora crassa, are also members of this family. The SPX domain of S. cerevisiae low-affinity phosphate transporters Pho87 and Pho90 auto-regulates uptake and prevents efflux. This SPX dependent inhibition is mediated by the physical interaction with Spl2. NUC-2 contains several ankyrin repeats. Several members of this family are annotated as XPR1 proteins: the xenotropic and polytropic retrovirus receptor confers susceptibility to infection with xenotropic and polytropic murine leukaemia viruses (MLV). Infection by these retroviruses can inhibit XPR1-mediated cAMP signaling and result in cell toxicity and death. The similarity between Syg1, phosphate regulators and XPR1 sequences has been previously noted, as has the additional similarity to several predicted proteins, of unknown function, from Drosophila melanogaster, Arabidopsis thaliana, Caenorhabditis elegans, Schizosaccharomyces pombe, S. cerevisiae, and many other diverse organisms.


Pssm-ID: 269894 [Multi-domain]  Cd Length: 143  Bit Score: 108.04  E-value: 2.36e-27
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 145326108   4 FGKYLQRKQIEEWSGYYINYKLMKKKVKQYAEQIQGGSQHP----------RHVLKDFSRMLDTQIETTVLF---MLEQQ 70
Cdd:cd14447    2 FGKRLREEAVPEWRDKYVDYKALKKLIKNLVASADEASNSSealelsesggEEFESEFFEALDAELEKVNEFyqeLLEEL 81

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|...
gi 145326108  71 GLLSGRLAKLRESHDAiLEQPDISRIFELREAYRDVGRDLLQLLKFVELNAIGLRKILKKFDK 133
Cdd:cd14447   82 QELLKRLEALEPDLPA-LRGSLKEELEDLRKELVESYSELEELERFVELNYTAFRKILKKYDK 143
MFS_MFSD8 cd17326
Major facilitator superfamily domain-containing protein 8; Major facilitator superfamily (MFS) ...
 249-657 7.05e-23

Major facilitator superfamily domain-containing protein 8; Major facilitator superfamily (MFS) domain-containing protein 8 (MFSD8) is also called ceroid-lipofuscinosis neuronal protein 7 (CLN7). It is a polytopic lysosomal membrane protein that may transport small solutes by using chemiosmotic ion gradients. Mutations in MFSD8/CLN7 cause a variant of late-infantile neuronal ceroid lipofuscinoses (vLINCL), a neurodegenerative lysosomal storage disorder. Some variants are associated with nonsyndromic autosomal recessive macular dystrophy. MFSD8/CLN7 belongs to the Eukaryotic Solute carrier 46 (SLC46)/Bacterial Tetracycline resistance -like (SLC46/TetA-like) family of the Major Facilitator Superfamily (MFS) of transporters. MFS proteins are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.


Pssm-ID: 340884 [Multi-domain]  Cd Length: 374  Bit Score: 101.13  E-value: 7.05e-23
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 145326108 249 SLLLNLGNTFLYMvntyIIVPTADDYSmsLGAAATVCGVVIGSMAVAQVFSSVYFSAWSNK--SYFKPLVFSSIALFIGN 326
Cdd:cd17326    8 MFLSSLQFSIVLT----SIWPYLQKID--PTATASFFGWVVAAYSLGQLVFSPVFGYWSNKtsSVKEPLIVSILIAIVGN 81

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 145326108 327 LMYALAYDA--NSIALLLLGRVCCGLG-SARAVNRRYISDCVPLRIRMQASAGFVSASALGMACGPALAGLL-----QIK 398
Cdd:cd17326   82 ILYALAEYFpsNRKYVMLVARFLIGVGaGNVAVLRAYVAAASTLKDRTRAMAVISAAQALGFIVGPALQAAFtpigyPGY 161

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 145326108 399 FKFYKFTFNQSTLPGWVMAVAWLFYLVWLCISFREplrdtedgeknnrnettsatdrvessrveeglrlpllitsgikpe 478
Cdd:cd17326  162 VIGIKLRFNMYTAPAWLMAILGIINIILILFFFKE--------------------------------------------- 196

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 145326108 479 deeecdeseespedshkpansfieayrlltpsvkVQLLIYFMLKYSMEILL-SESSVITSYYFSWT----TSSVAIFLAC 553
Cdd:cd17326  197 ----------------------------------VVCIFLFFVVLFIFANLeTIGTPLTMDMYGWTreeaVLYNGIILAV 242

                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 145326108 554 LGLTVLPINILVGSYISNMFEDRQILLTSEIIVFLGILFsfnlfvpyTVPQYVISGLIMFVAAEVLEGVNLSLLSRVMSS 633
Cdd:cd17326  243 SGVLSVLVFLLFKFLSLGLIDERKILLGGLILFFLGFVV--------TLPWGITGVILFGLGYPIGNVMLYTLYSKILGP 314

                        410       420
                 ....*....|....*....|....
gi 145326108 634 RlSKGTYNGGLlsTEAGTLARVVA 657
Cdd:cd17326  315 K-PQGTMQGVL--TASGSLARTLG 335
SPX_PHO81_NUC-2_like cd14483
SPX domain of Pho81, NUC-2, and similar proteins; This region has been named the SPX domain ...
 4-134 4.34e-21

SPX domain of Pho81, NUC-2, and similar proteins; This region has been named the SPX domain after (Syg1, Pho81 and XPR1). The domain is found at the amino terminus of a variety of proteins. The N-termini of several proteins involved in the regulation of phosphate transport, including the putative phosphate level sensors Pho81 from Saccharomyces cerevisiae and NUC-2 from Neurospora crassa, are also members of this family. NUC-2 plays an important role in the phosphate-regulated signal transduction pathway in N. crassa. It shows high similarity to a cyclin-dependent kinase inhibitory protein Pho81, which is part of the phosphate regulatory cascade in S. cerevisiae. Both, NUC-2 and Pho81, have multi-domain architecture, including the SPX N-terminal domain following by several ankyrin repeats and a putative C-terminal glycerophosphodiester phosphodiesterase domain (GDPD) with unknown function.


Pssm-ID: 269904 [Multi-domain]  Cd Length: 162  Bit Score: 90.39  E-value: 4.34e-21
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 145326108   4 FGKYLQRKQIE--EWSGYYINYKLMKKKVKQYA-------------------EQIQGGSQHPRhvLKD----FSRMLDTQ 58
Cdd:cd14483    2 FGKYIQARQLElpEYSAYFLDYKALKKLIKSLAaprvaaaaallaggrplspDGTDESDAQTS--LQAnkaaFFFKLERE 79

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 145326108  59 IETTVLFMLEQQGLLSGRLAKLRESHDAILEQ--PDISRIFE---LREAYRDVGRDLLQLLKFVELNAIGLRKILKKFDK 133
Cdd:cd14483   80 LEKVNAFYLQKEAELKLRLDTLLDKKRVLQSRgkLASKKSASfvtLEEGFRQFERDLNKLQQFVELNATGFSKILKKWDK 159


                 .
gi 145326108 134 R 134
Cdd:cd14483  160 R 160
SPX_GDE1_like cd14484
SPX domain of Gde1 and similar proteins; This region has been named the SPX domain after (Syg1, ...
 4-136 3.75e-20

SPX domain of Gde1 and similar proteins; This region has been named the SPX domain after (Syg1, Pho81 and XPR1). The domain is found at the amino terminus of a variety of proteins. The N-termini of several proteins involved in the regulation of phosphate transport, including the putative phosphate level sensors Pho81 from Saccharomyces cerevisiae and NUC-2 from Neurospora crassa, are also members of this family. The yeast protein Gde1/Ypl110c is similar to both, NUC-2 and Pho81, in sharing their multi-domain architecture, which includes the SPX N-terminal domain followed by several ankyrin repeats and a C-terminal glycerophosphodiester phosphodiesterase domain (GDPD). Gde1 hydrolyzes intracellular glycerophosphocholine into glycerolphosphate and choline, and plays a role in the utilization of glycerophosphocholine as a source for phosphate.


Pssm-ID: 269905 [Multi-domain]  Cd Length: 134  Bit Score: 86.82  E-value: 3.75e-20
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 145326108   4 FGKYLQRKQIEEWSGYYINYKLMKKKVKQYAEQIQGGSQhprHVLKDFSRMLDTQIETTVLFMLEQQGLLSGRLAKLRES 83
Cdd:cd14484    2 FGKNLPRNQVPEWSSSYINYKGLKKLIKAIAEQQKEGVK---VDLAEFFFALDRNLEDVDTFYNKKFAEYSRRLKLLLDR 78

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|...
gi 145326108  84 H------DAILEQPDISRI----FELREAYRDvgrdllqLLKFVELNAIGLRKILKKFDKRFG 136
Cdd:cd14484   79 YgfspdlVQNLDSDELEELmgalLELRSQLRN-------LQWFGELNRRGFVKILKKLDKKVP 134
SPX_SYG1_like cd14475
SPX domain of the yeast plasma protein Syg1 and related proteins; This region has been named ...
 4-133 8.41e-15

SPX domain of the yeast plasma protein Syg1 and related proteins; This region has been named the SPX domain after (Syg1, Pho81 and XPR1). The domain is found at the amino terminus of a variety of proteins. In the yeast protein Syg1, the N-terminus binds directly to the G-protein beta subunit and inhibits transduction of the mating pheromone signal, and it co-occurs with a C-terminal domain from the EXS family.


Pssm-ID: 269896 [Multi-domain]  Cd Length: 139  Bit Score: 71.83  E-value: 8.41e-15
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 145326108   4 FGKYLQRKQIEEWSGYYINYKLMKKKVKQyaeqiqggsqhprhvlKDFSRMLDTQIETTVLF--MLEQQ-----GLLSGR 76
Cdd:cd14475    2 FAKYLEENLVPEWRKKYLDYKGGKKKIKA----------------REFFEFLDSELDKVESFykEKEDEarerlDLLRDQ 65

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 145326108  77 LAKLRESHD--AILEQPDISR----IFELREAYRDVGRDL----------LQLLK-FVELNAIGLRKILKKFDK 133
Cdd:cd14475   66 LHELRDHRIqeADDGRRDYSRrpeqNAHDPVSYRSARRKLkkalqeyyrgLELLKsYRLLNRTAFRKINKKFDK 139
AraJ COG2814
Predicted arabinose efflux permease AraJ, MFS family [Carbohydrate transport and metabolism];
249-646 1.38e-13

Predicted arabinose efflux permease AraJ, MFS family [Carbohydrate transport and metabolism];


Pssm-ID: 442063 [Multi-domain]  Cd Length: 348  Bit Score: 72.70  E-value: 1.38e-13
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 145326108 249 SLLLNLGNTFLYMVNTYIIVPTADDYSMSLGAAATVCGVVIGSMAVAQVFSSVYFSAWSNKSYFKPLVFSSIALF-IGNL 327
Cdd:COG2814   11 ALLALALGAFLSGLGIGIVLPALPLIAADLGASPAQAGLVVTAYLLGAALGAPLAGRLADRFGRRRVLLLGLLLFaLGSL 90

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 145326108 328 MYALaydANSIALLLLGRVCCGLGSA--RAVNRRYISDCVPLRIRMQASAGFVSASALGMACGPALAGLLqikfkfykft 405
Cdd:COG2814   91 LCAL---APSLWLLLAARFLQGLGAGalFPAALALIADLVPPERRGRALGLLGAGLGLGPALGPLLGGLL---------- 157

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 145326108 406 fNQSTLPGWVMAVAWLFYLV-WLCISFREPlrdtedgeknnrnETTSATDRVESSRVEEGLRLPLLITSgikpedeeecd 484
Cdd:COG2814  158 -ADLFGWRWVFLVNAVLALLaLLLLLRLLP-------------ESRPAARARLRGSLRELLRRPRLLLL----------- 212

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 145326108 485 eseespedshkpansfieayrlltpsvkvqLLIYFMLKYSMEILLSESSVITSYYFSWTTSSVAIFLACLGLTVLPINIL 564
Cdd:COG2814  213 ------------------------------LLLAFLLGFGFFALFTYLPLYLQEVLGLSASAAGLLLALFGLGGVLGALL 262

                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 145326108 565 VGsYISNMFEDRQILLTSEIIVFLGILFsfnLFVPYTVPQYVISGLIMFVAAEVLEGVNLSLLSRVMSSRLsKGTYNGGL 644
Cdd:COG2814  263 AG-RLADRFGRRRLLLIGLLLLALGLLL---LALAGSLWLLLLALFLLGFGFGLLFPLLQALVAELAPPEA-RGRASGLY 337


                 ..
gi 145326108 645 LS 646
Cdd:COG2814  338 NS 339
MFS_MdtG_SLC18_like cd17325
bacterial MdtG-like and eukaryotic solute carrier 18 (SLC18) family of the Major Facilitator ...
 257-461 3.93e-13

bacterial MdtG-like and eukaryotic solute carrier 18 (SLC18) family of the Major Facilitator Superfamily of transporters; This family is composed of eukaryotic solute carrier 18 (SLC18) family transporters and related bacterial multidrug resistance (MDR) transporters including several proteins from Escherichia coli such as multidrug resistance protein MdtG, from Bacillus subtilis such as multidrug resistance proteins 1 (Bmr1) and 2 (Bmr2), and from Staphylococcus aureus such as quinolone resistance protein NorA. The family also includes Escherichia coli arabinose efflux transporters YfcJ and YhhS. MDR transporters are drug/H+ antiporters (DHA) that mediate the efflux of a variety of drugs and toxic compounds, and confer resistance to these compounds. The SLC18 transporter family includes vesicular monoamine transporters (VAT1 and VAT2), vesicular acetylcholine transporter (VAChT), and SLC18B1, which is proposed to be a vesicular polyamine transporter (VPAT). The MdtG/SLC18 family belongs to the Major Facilitator Superfamily (MFS) of membrane transport proteins, which are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.


Pssm-ID: 340883 [Multi-domain]  Cd Length: 375  Bit Score: 71.45  E-value: 3.93e-13
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 145326108 257 TFLYMVNTYIIVPTADDYSMSLGAAATVCGVVIGSMAVAQVFSSVYFSAWSNK-SYFKPLVFSSIALFIGNLMYALaydA 335
Cdd:cd17325    5 LFLDMLGYGIIIPILPLYAESLGASVAQIGLIVAAYALAQLLFSPPAGRLSDRfGRKRLLLLGLLLLAVSTLLFAF---A 81

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 145326108 336 NSIALLLLGRVCCGLGSARA--VNRRYISDCVPLRIRMQASAGFVSASALGMACGPALAGLLqIKFKFYKFTFnqstlpg 413
Cdd:cd17325   82 TSYWQLLLARFLQGLASAAVwpAAMALIADIAPPEERGRAMGIFSSAIGLGFLIGPLLGGLL-ADALGYRAPF------- 153

                        170       180       190       200
                 ....*....|....*....|....*....|....*....|....*...
gi 145326108 414 WVMAVAWLFYLVWLCISFREPLRDTEDGEKNNRNETTSATDRVESSRV 461
Cdd:cd17325  154 LVCAALALLALVLALLLLPEPRPPPNKRAVSAARLRSGLRLLLRDRRL 201
MFS_SLC46_TetA_like cd17330
Eukaryotic Solute carrier 46 (SLC46) family, Bacterial Tetracycline resistance proteins, and ...
 258-663 4.43e-12

Eukaryotic Solute carrier 46 (SLC46) family, Bacterial Tetracycline resistance proteins, and similar proteins of the Major Facilitator Superfamily of transporters; This family is composed of the eukaryotic proteins MFSD9, MFSD10, MFSD14, and SLC46 family proteins, as well as bacterial multidrug resistance (MDR) transporters such as tetracycline resistance protein TetA and multidrug resistance protein MdtG. MDR transporters are drug/H+ antiporters (DHA) that mediate the efflux of a variety of drugs and toxic compounds, and confer resistance to these compounds. TetA proteins confer resistance to tetracycline while MdtG confers resistance to fosfomycin and deoxycholate. The Solute carrier 46 (SLC46) family is composed of three vertebrate members (SLC46A1, SLC46A2, and SLC46A3), the best-studied of which is SLC46A1, which functions both as an intestinal proton-coupled high-affinity folate transporter involved in the absorption of folates and as an intestinal heme transporter which mediates heme uptake. MFSD10 facilitates the uptake of organic anions such as some non-steroidal anti-inflammatory drugs (NSAIDs) and confers resistance to such NSAIDs. The SLC46/TetA-like family belongs to the Major Facilitator Superfamily (MFS) of membrane transport proteins, which are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.


Pssm-ID: 340888 [Multi-domain]  Cd Length: 349  Bit Score: 67.99  E-value: 4.43e-12
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 145326108 258 FLYMVNTYIIVPTADDYSMS---LGAAATVCGVVIGSMAVAQVFSSVYFSAWSNKSYFKP-LVFSSIALFIGNLMYALAy 333
Cdd:cd17330    6 FLDALGFGIVFPVLPFYVREfnkLGASGYYVGLLQSSYSLGQFLASPLWGRLSDRYGRKPvLLLSLAGSAVSYVLLGLS- 84

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 145326108 334 daNSIALLLLGRVCCGLGSAR-AVNRRYISDCVPLRIRMQASAGFVSASALGMACGPALAGLLQikfkfykfTFNQSTLP 412
Cdd:cd17330   85 --RNLWLLFLARFLDGLTGGNvSVAQAYIADITDEEERAKAFGIISAAFGLGFILGPALGGFLS--------TPYGYALP 154

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 145326108 413 GWVMAVAWLFylvwlcisfreplrdtedgeknnrnettsatdrvessrveeglrlpllitsgikpedeeecdeseesped 492
Cdd:cd17330  155 FLVAAALALL---------------------------------------------------------------------- 164

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 145326108 493 shkpanSFIEAYRLLtPSVKVQLLIYFMLKYSMEILLSESSVITSYYFSWTTSSVAIFLACLGLTVLPINILVGSYISNM 572
Cdd:cd17330  165 ------NLVLVLFFL-PPLRRLLLVYFLIALAFAGLESVFSLFLKDRFGFSPSQIGLLLALVGIAGILAQLLLIPRLAKR 237

                        330       340       350       360       370       380       390       400
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 145326108 573 FEDRQILLTSEIIVFLGilfsfNLFVPY--TVPQYVISGLIMFVAAEVLEGVNLSLLSRVMSSRlSKGTYNGGLLSteAG 650
Cdd:cd17330  238 LGERKLLRLGLLLAIIG-----LLLLPFasSVPLLVLAAAILAFGTGIVFPALSALLSKSVPSK-EQGKAQGIIQS--LQ 309

                        410
                 ....*....|...
gi 145326108 651 TLARVVADATITL 663
Cdd:cd17330  310 SLARIIGPLLAGP 322
SPX_VTC2_like cd14480
SPX domain of the vacuolar transport chaperone Vtc2 and similar proteins; This region has been ...
 4-133 3.33e-11

SPX domain of the vacuolar transport chaperone Vtc2 and similar proteins; This region has been named the SPX domain after (Syg1, Pho81 and XPR1). The domain is found at the amino terminus of a variety of proteins. Vtc2 is part of the Saccharomyces cerevisiae membrane-integral VTC complex, together with Vtc1, Vtc3, and Vtc4. It contains an N-terminal SPX domain next to a central polyphosphate polymerase domain and a C-terminal domain of unknown function.


Pssm-ID: 269901 [Multi-domain]  Cd Length: 135  Bit Score: 61.40  E-value: 3.33e-11
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 145326108   4 FGKYLQRKQIEEWSGYYINYKLMKKKVKQYAEQIQGGSQhprhvlKD---FSRMLDTQIETTVLFMLEQQGLLSGRLAKL 80
Cdd:cd14480    2 FGKTLKSSIYPPWKDYYIDYDKLKKLLKERETDRGWWTE------DDerfFVELLEVELEKVYTFQKEKYSELRRRIDAC 75

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|
gi 145326108  81 RESHDAILEQPDISRIFELREAYRDVGRDLLQLL-------KFVELNAIGLRKILKKFDK 133
Cdd:cd14480   76 EKKVKELVSNLDSSEDDPSEEDFKELEEELDDILadvhdlaKFTRLNYTGFLKIVKKHDK 135
SPX_AtSPX1_like cd14481
SPX domain of the plant protein SPX1 and similar proteins; This region has been named the SPX ...
 4-134 3.83e-11

SPX domain of the plant protein SPX1 and similar proteins; This region has been named the SPX domain after (Syg1, Pho81 and XPR1). The domain is found at the amino terminus of a variety of proteins. This family of plant proteins contains a single SPX domain. Arabidopsis thaliana SPX1 and SPX3 have been reported to play roles in the adaptation to low-phosphate conditions, SPX3 may be involved in the regulation of SPX1 activity. Oryza sativa SPX1 suppresses the regulation of expression of OsPT2, a low-affinity phosphate transporter, by the MYB-like OsPHR2.


Pssm-ID: 269902 [Multi-domain]  Cd Length: 149  Bit Score: 61.52  E-value: 3.83e-11
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 145326108   4 FGKYLQRkQIE----EWSGYYINYKLMKKKVKQYAE---------QIQGGSQHPRHVLK---DFSRMLDTQIETTVLFML 67
Cdd:cd14481    2 FGKSLKR-QIEetlpEWRDKFLSYKELKKLLKLISPgnadkpnskRDRRGGGAARAMTKeeaDFVRLLNAELDKFNAFFV 80

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 145326108  68 EQQGLLSGRLAKLRESHDAILEQPDISRifelrEAYRDVGRDLLQ-------LLKFVELNAIGLRKILKKFDKR 134
Cdd:cd14481   81 EKEEEYVIRLKELQDRVAEAKETPRDSN-----EELMRIRREIVDfhgemvlLENYSSLNYTGLVKILKKYDKR 149
MFS_1 pfam07690
Major Facilitator Superfamily;
257-642 6.55e-10

Major Facilitator Superfamily;


Pssm-ID: 429598 [Multi-domain]  Cd Length: 344  Bit Score: 61.28  E-value: 6.55e-10
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 145326108  257 TFLYMVNTYIIVPTADDYSM-SLGAAATVCGVVIGSMAVAQVFSSVYFSAWSNKSYFKPLVFSSIALFIgnLMYALAYDA 335
Cdd:pfam07690   5 AFLAALGRSLLGPALPLLLAeDLGISPTEIGLLLTLFSLGYALAQPLAGRLSDRFGRRRVLLIGLLLFA--LGLLLLLFA 82

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 145326108  336 NSIALLLLGRVCCGLGSA--RAVNRRYISDCVPLRIRMQASAGFVSASALGMACGPALAGLLqIKFKFYKFTFnqstlpg 413
Cdd:pfam07690  83 SSLWLLLVLRVLQGLGAGalFPAALALIADWFPPEERGRALGLVSAGFGLGAALGPLLGGLL-ASLFGWRAAF------- 154

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 145326108  414 WVMAVAWLFYLVWLCISFREPLRDTEDGEKnnrnettsatdrvessrveeglrlpllitsgikpedeeecdeseespeds 493
Cdd:pfam07690 155 LILAILSLLAAVLLLLPRPPPESKRPKPAE-------------------------------------------------- 184

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 145326108  494 hKPANSFIEAYRLLTPSVKVQLLIYFMLKYSMEILLSESSVITSYYFSWTTSSVAIFLACLGLTVLPINILVGsYISNMF 573
Cdd:pfam07690 185 -EARLSLIVAWKALLRDPVLWLLLALLLFGFAFFGLLTYLPLYQEVLGLSALLAGLLLGLGGLLGAIGRLLLG-RLSDRL 262

                         330       340       350       360       370       380
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 145326108  574 EDRQILLTSEIIVFLGILFSFNLFVPYTVPQYVISGLIMFVAAEVLEGVNLSLLSRVMSSRLSkGTYNG 642
Cdd:pfam07690 263 GRRRRLLLALLLLILAALGLLLLSLTLSSLWLLLALLLLGFGFGLVFPALNALVSDLAPKEER-GTASG 330
SPX_YDR089W cd14474
SPX domain of the yeast protein YDR089W and related proteins; This region has been named the ...
 4-133 1.03e-09

SPX domain of the yeast protein YDR089W and related proteins; This region has been named the SPX domain after (Syg1, Pho81 and XPR1). The domain is found at the amino terminus of a variety of proteins. The uncharacterized yeast protein YDR089W has not been shown to be involved in phosphate homeostasis, in contrast to most of the other SPX-domain containing proteins.


Pssm-ID: 269895 [Multi-domain]  Cd Length: 144  Bit Score: 57.24  E-value: 1.03e-09
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 145326108   4 FGKYLQRKQIEEWSGYYINYKLMKKKVKQYAEQIQGGS----QHPRHVLKDFSRMLDTQIETTVLFMLEQQGLLSGRLAK 79
Cdd:cd14474    2 FGEQLLQRSVPEWKLYNIDYNELKHLIKEHTTRDQGTAiaipSALEKFEDSLYNEFCEQFDRVNLFVSSKADEISRRLEH 81

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|...
gi 145326108  80 LRESHDAILEQ------PDISRIFELREAYRDV---GRDLLQLLKFVELNAIGLRKILKKFDK 133
Cdd:cd14474   82 LESSILRLLERsasnsgSRRRQKRRLAKIEQELlrcGEELQKLSRFIIAQKIAFRKILKKYKK 144
SPX_PHO87_PHO90_like cd14478
SPX domain of the phosphate transporters Pho87, Pho90, Pho91, and related proteins; This ...
 15-133 1.93e-09

SPX domain of the phosphate transporters Pho87, Pho90, Pho91, and related proteins; This region has been named the SPX domain after (Syg1, Pho81 and XPR1). The domain is found at the amino terminus of a variety of proteins. The SPX domain of the Saccharomyces cerevisiae membrane-localized low-affinity phosphate transporters Pho87 and Pho90 auto-regulates uptake and prevents efflux. This SPX dependent inhibition is mediated by the physical interaction with Spl2. Pho91 is involved in the export of inorganic phosphate from the vacuole to the cytosol. While both, Pho87 and Pho90, transport phosphate into the cell, only Pho87 appears to also function as a sensor for high extracellular phosphate concentrations.


Pssm-ID: 269899 [Multi-domain]  Cd Length: 148  Bit Score: 56.78  E-value: 1.93e-09
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 145326108  15 EWSGYYINYKLMKKKV----KQYAEQIQGGSQHPRHVL------------KDFSRMLDTQIETTVLFMLEQQGLLSGRLA 78
Cdd:cd14478   13 EWSDHYIAYSNLKKLIyqleKDQLQLQNGGDDEEEEESsllllstdedpdDVFVRALDKELEKIDSFYKEKEAELYAEVD 92

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 145326108  79 KLREshdaileqpDISRiFELREAYRDVGRDL--------LQL--LK-FVELNAIGLRKILKKFDK 133
Cdd:cd14478   93 ELLK---------DVEE-FEEENYLYDSRISLkkriinlyVSLseLKsYIELNRTGFSKILKKYDK 148
SPX pfam03105
SPX domain; We have named this region the SPX domain after SYG1, Pho81 and XPR1. This 180 ...
 108-147 1.70e-08

SPX domain; We have named this region the SPX domain after SYG1, Pho81 and XPR1. This 180 residue long domain is found at the amino terminus of a variety of proteins. In the yeast protein SYG1, the N-terminus directly binds to the G-protein beta subunit and inhibits transduction of the mating pheromone signal. Similarly, the N-terminus of the human XPR1 protein binds directly to the beta subunit of the G-protein heterotrimer leading to increased production of cAMP. These findings suggest that all the members of this family are involved in G-protein associated signal transduction. The N-termini of several proteins involved in the regulation of phosphate transport, including the putative phosphate level sensors PHO81 from Saccharomyces cerevisiae and NUC-2 from Neurospora crassa, are also members of this family. The SPX domain of S. cerevisiae low-affinity phosphate transporters Pho87 and Pho90 auto-regulates uptake and prevents efflux. This SPX dependent inhibition is mediated by the physical interaction with Spl2 NUC-2 contains several ankyrin repeats pfam00023. Several members of this family are annotated as XPR1 proteins: the xenotropic and polytropic retrovirus receptor confers susceptibility to infection with murine xenotropic and polytropic leukaemia viruses (MLV). Infection by these retroviruses can inhibit XPR1-mediated cAMP signalling and result in cell toxicity and death. The similarity between SYG1, phosphate regulators and XPR1 sequences has been previously noted, as has the additional similarity to several predicted proteins, of unknown function, from Drosophila melanogaster, Arabidopsis thaliana, Caenorhabditis elegans, Schizosaccharomyces pombe, and Saccharomyces cerevisiae, and many other diverse organizms. In addition, given the similarities between XPR1 and SYG1 and phosphate regulatory proteins, it has been proposed that XPR1 might be involved in G-protein associated signal transduction and may itself function as a phosphate sensor.


Pssm-ID: 460807 [Multi-domain]  Cd Length: 339  Bit Score: 56.80  E-value: 1.70e-08
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|
gi 145326108  108 RDLLQLLKFVELNAIGLRKILKKFDKRFGYRFADYYVKTR 147
Cdd:pfam03105 300 RGLELLKSYSELNRTAFRKILKKFDKVTSLNASKDYMKEV 339
MFS_YfcJ_like cd17489
Escherichia coli YfcJ, YhhS, and similar transporters of the Major Facilitator Superfamily; ...
 252-396 2.15e-08

Escherichia coli YfcJ, YhhS, and similar transporters of the Major Facilitator Superfamily; This subfamily is composed of Escherichia coli membrane proteins, YfcJ and YhhS, Bacillus subtilis uncharacterized MFS-type transporter YwoG, and similar proteins. YfcJ and YhhS are putative arabinose efflux transporters. YhhS has been implicated glyphosate resistance. YfcJ-like arabinose efflux transporters belong to the bacterial MdtG-like and eukaryotic solute carrier 18 (SLC18) family of the Major Facilitator Superfamily (MFS) of transporters. MFS proteins are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.


Pssm-ID: 341042 [Multi-domain]  Cd Length: 367  Bit Score: 56.83  E-value: 2.15e-08
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 145326108 252 LNLGNTFLYMVNtYIIVPTADDYSMSLGAAATVCGVVIGSMAVAQVFSSVYFSAWSNKSYFKPLVFSSIALF-IGNLMYA 330
Cdd:cd17489    1 ILLANFFFFLSF-YLLLPVLPLYAHDLGGSEAVAGLVVGLFTLAALLARPFAGRLLDRFGRKRLLLIGLLIFaLATLLYL 79

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 145326108 331 LaydANSIALLLLGRVCCGLG--SARAVNRRYISDCVPLRIRMQASAGFVSASALGMACGPALAGLLQ 396
Cdd:cd17489   80 L---ATSVALLLVLRLIHGIGwgAFTTAAATLVADIIPPSRRGEGIGYYGLATTLAMALGPALGLFLY 144
MFS_MdtG_SLC18_like cd17325
bacterial MdtG-like and eukaryotic solute carrier 18 (SLC18) family of the Major Facilitator ...
 249-395 5.28e-08

bacterial MdtG-like and eukaryotic solute carrier 18 (SLC18) family of the Major Facilitator Superfamily of transporters; This family is composed of eukaryotic solute carrier 18 (SLC18) family transporters and related bacterial multidrug resistance (MDR) transporters including several proteins from Escherichia coli such as multidrug resistance protein MdtG, from Bacillus subtilis such as multidrug resistance proteins 1 (Bmr1) and 2 (Bmr2), and from Staphylococcus aureus such as quinolone resistance protein NorA. The family also includes Escherichia coli arabinose efflux transporters YfcJ and YhhS. MDR transporters are drug/H+ antiporters (DHA) that mediate the efflux of a variety of drugs and toxic compounds, and confer resistance to these compounds. The SLC18 transporter family includes vesicular monoamine transporters (VAT1 and VAT2), vesicular acetylcholine transporter (VAChT), and SLC18B1, which is proposed to be a vesicular polyamine transporter (VPAT). The MdtG/SLC18 family belongs to the Major Facilitator Superfamily (MFS) of membrane transport proteins, which are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.


Pssm-ID: 340883 [Multi-domain]  Cd Length: 375  Bit Score: 55.66  E-value: 5.28e-08
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 145326108 249 SLLLNLGNTFLYMVNTYIIVPTADDYSMSLGAA-ATVCGVVIGSMAVAQVFSSVYFSAWSNKSYFKPLVFssIALFIGNL 327
Cdd:cd17325  200 RLLALFLAIFVLAFAFGALEPFLPLYAAELGGLsPAQIGLLFGAQGLASALSQPPAGKLSDRIGRKPLIL--IGLLLSAV 277

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 145326108 328 MYALAYDANSIALLLLGRVCCGLGSA--RAVNRRYISDCVPLRIRMQASAGFVSASALGMACGPALAGLL 395
Cdd:cd17325  278 ALLLLPLATSFWLLLLLLALLGLGLGlvFPATLALLADIVPPEGRGTAMGLFNTAFSLGMVLGPLLGGFL 347
MFS_MdtH_MDR_like cd17329
Multidrug resistance protein MdtH and similar multidrug resistance (MDR) transporters of the ...
 246-433 1.27e-07

Multidrug resistance protein MdtH and similar multidrug resistance (MDR) transporters of the Major Facilitator Superfamily; This family is composed of Escherichia coli MdtH and similar multidrug resistance (MDR) transporters from bacteria and archaea, many of which remain uncharacterized. MDR transporters are drug/H+ antiporters (DHA) that mediate the efflux of a variety of drugs and toxic compounds, and confer resistance to these compounds. MdtH confers resistance to norfloxacin and enoxacin. MdtH-like MDR transporters belong to the Major Facilitator Superfamily (MFS) of membrane transport proteins, which are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.


Pssm-ID: 340887 [Multi-domain]  Cd Length: 376  Bit Score: 54.54  E-value: 1.27e-07
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 145326108 246 HFNSLLLNLGntfLYMVNTYIIVPTADDYSMSLGAAatvcGVVIGSMAVAQVFSSVYFSAWSNKSYFKPLVFssIALFIG 325
Cdd:cd17329    5 LIGSFLNRLG---FFMVWPFMAIYLHQQLGLSASIV----GLVLALSAVAGIVASLIGGRLADRFGRKPVML--AGLLLR 75

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 145326108 326 NLMYALAYDANSIALLLLGRVCCGLGSA--RAVNRRYISDCVPLRIRMQASAGFVSASALGMACGPALAGLLqikfkfYK 403
Cdd:cd17329   76 ALGFALLGFAHSPWLFAIALVLTGFGGGlfEPASEAMIADVTTPENRTRAFSLLYWAINLGVAIGPLLGGLL------LL 149

                        170       180       190
                 ....*....|....*....|....*....|
gi 145326108 404 FTFNqstLPGWVMAVAWLFYLVWLCISFRE 433
Cdd:cd17329  150 YSFR---LLFLAAAVLFLLAALVLLFLLPE 176
MFS_MFSD9 cd17390
Major facilitator superfamily domain-containing protein 9; Major facilitator superfamily ...
 266-431 3.57e-07

Major facilitator superfamily domain-containing protein 9; Major facilitator superfamily domain-containing protein 9 (MFSD9) is expressed in the central nervous system (CNS) and in most peripheral tissues but at very low expression levels. The function of MFSD9 is unknown. MFSD9 belongs to the Eukaryotic Solute carrier 46 (SLC46)/Bacterial Tetracycline resistance (TetA) -like (SLC46/TetA-like) family of the Major Facilitator Superfamily (MFS) of transporters. MFS proteins are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.


Pssm-ID: 340948 [Multi-domain]  Cd Length: 350  Bit Score: 53.00  E-value: 3.57e-07
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 145326108 266 IIVPTADDYSMSLGAAATVCGVVIGSMAVAQVFSSVYFSAWSNKSYFKPLVFSSIAL-FIGnlmYALAYDANSIALLLLG 344
Cdd:cd17390   14 MILPLLSTHIRSLGASPTVIGLIGSVYGALQLFSSPLVGSWSDVVGRRPVLLICLLLtALG---YVLLGFSTSLALLFLS 90

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 145326108 345 RVCCGL-GSARAVNRRYISDCVPLRIRMQASAGFVSASALGMACGPALAGLL-QIKFKFYKFTFnqstlpgwvmAVAWLF 422
Cdd:cd17390   91 RIPLGIfKHTQSLCKAYLSDLVSEQERSRVLGRFNAASSLGFILGPVVGGHLaETDGGFYYVAL----------LCSAIF 160

                        170
                 ....*....|...
gi 145326108 423 Y----LVWLCISF 431
Cdd:cd17390  161 IlnagLVWFLLSK 173
ProP COG0477
MFS family permease, includes anhydromuropeptide permease AmpG [Carbohydrate transport and ...
 257-395 1.72e-06

MFS family permease, includes anhydromuropeptide permease AmpG [Carbohydrate transport and metabolism, Amino acid transport and metabolism, Inorganic ion transport and metabolism, General function prediction only];


Pssm-ID: 440245 [Multi-domain]  Cd Length: 295  Bit Score: 50.19  E-value: 1.72e-06
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 145326108 257 TFLYMVNTYIIVPTADDYSMSLGAAATVCGVVIGSMAVAQVFSSVYFSAWSNKSYFKPLVFSSIALFIgnLMYALAYDAN 336
Cdd:COG0477   23 TFLEGLDFTIVNVALPSIAADLGASSAQLGWIVSAYLLGRAIGLLLFGRLGDRYGRKRVLLIGLLLFG--LASLLCGLAP 100

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|.
gi 145326108 337 SIALLLLGRVCCGLGSA--RAVNRRYISDCVPLRIRMQASAGFVSASALGMACGPALAGLL 395
Cdd:COG0477  101 SPELLIAARALQGIGAGglMPGALALIAELFPARERGRALGLWGAAIGLGLALGPLLGGLL 161
MFS_NepI_like cd17324
Purine ribonucleoside efflux pump NepI and similar transporters of the Major Facilitator ...
 257-395 2.11e-06

Purine ribonucleoside efflux pump NepI and similar transporters of the Major Facilitator Superfamily; This family is composed of purine efflux pumps such as Escherichia coli NepI and Bacillus subtilis PbuE, sugar efflux transporters such as Corynebacterium glutamicum arabinose efflux permease, multidrug resistance (MDR) transporters such as Streptomyces lividans chloramphenicol resistance protein (CmlR), and similar proteins. NepI and PbuE are involved in the efflux of purine ribonucleosides such as guanosine, adenosine and inosine, as well as purine bases like guanine, adenine, and hypoxanthine, and purine base analogs. They play a role in the maintenance of cellular purine base pools, as well as in protecting the cells and conferring resistance against toxic purine base analogs such as 6-mercaptopurine. MDR transporters are drug/H+ antiporters (DHA) that mediate the efflux of a variety of drugs and toxic compounds, and confer resistance to these compounds. The NepI-like family belongs to the Major Facilitator Superfamily (MFS) of membrane transport proteins, which are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.


Pssm-ID: 340882 [Multi-domain]  Cd Length: 370  Bit Score: 50.63  E-value: 2.11e-06
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 145326108 257 TFLYMVNTYIIVP----TADDYSMSLGAAATVCGVVIGSMAVAQVFSSVYFSAWSNKsyfKPLVFSSIALFIGNLMYALA 332
Cdd:cd17324    8 AFAIGTTEYVIQGllpdIARDFGVSVAQAGLLVSAYALGYALGALLLGPLSDRIERR---RLLLVLLLLFILGNLLAALA 84

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 145326108 333 ydaNSIALLLLGRVCCGLGSAR--AVNRRYISDCVPLRIRMQASAGFVSASALGMACGPALAGLL 395
Cdd:cd17324   85 ---PSFALLLLARALAGLAHGGfwAIAAAYAADLVPPEKRGRAIGLVFSGLTLGLVLGRPLGGLL 146
MFS_YajR_like cd17472
Escherichia coli inner membrane transport protein YajR and similar multidrug-efflux ...
 258-402 2.85e-06

Escherichia coli inner membrane transport protein YajR and similar multidrug-efflux transporters of the Major Facilitator Superfamily; This family is composed of Escherichia coli inner membrane transport protein YajR and some uncharacterized multidrug-efflux transporters. YajR is a putative proton-driven major facilitator superfamily (MFS) transporter found in many gram-negative bacteria. Unlike most MFS transporters, YajR contains a C-terminal, cytosolic YAM domain, which may play an essential role for the proper functioning of the transporter. YajR-like transporters belong to the Major Facilitator Superfamily (MFS) of membrane transport proteins, which are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.


Pssm-ID: 341025 [Multi-domain]  Cd Length: 371  Bit Score: 49.91  E-value: 2.85e-06
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 145326108 258 FLYMVNTYIIVPTADDYSMSL-GAAATVCGVVIGSMAVAQVFSSVYFSAWSNKSYFKPLVFSSIALFI-GNLmyaLAYDA 335
Cdd:cd17472    6 SLRMLGMFLVLPVLSVYAKSLpGATPTLIGLALGIYGLTQAIFQIPFGLLSDRIGRKPVILFGLLLFIaGSL---LAAYA 82

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 145326108 336 NSIALLLLGRVCCGLGSARAVNRRYISDCVPLRIRMQASAgFVSAS-----ALGMACGPALAGLLQIKFKFY 402
Cdd:cd17472   83 TSIYGLILGRALQGSGAISSVVMALLADLTREENRTKAMA-LIGASigiafAISMVLGPLLAAHLGVSGLFL 153
MFS_TetA cd17388
Tetracycline resistance protein TetA and related proteins of the Major Facilitator Superfamily ...
 267-594 3.73e-06

Tetracycline resistance protein TetA and related proteins of the Major Facilitator Superfamily of transporters; This subfamily is composed of tetracycline resistance proteins similar to Escherichia coli TetA(A), TetA(B), and TetA(E), which are metal-tetracycline/H(+) antiporters that confer resistance to tetracycline by an active tetracycline efflux, which is an energy-dependent process that decreases the accumulation of the antibiotic in cells. TetA-like tetracycline resistance proteins belongs to the Eukaryotic Solute carrier 46 (SLC46)/Bacterial Tetracycline resistance (TetA) -like (SLC46/TetA-like) family of the Major Facilitator Superfamily (MFS) of transporters. MFS proteins are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.


Pssm-ID: 340946 [Multi-domain]  Cd Length: 385  Bit Score: 49.94  E-value: 3.73e-06
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 145326108 267 IVPT-ADDYSMSLGAAATVCGVVIGSMAVAQVFSSVYFSAWSNKSYFKPLVFSSIALFigNLMYALAYDANSIALLLLGR 345
Cdd:cd17388   21 VLPTlLREFVGDSADAATHYGVLLALYALMQFIFAPILGALSDRFGRRPVLLLSLAGA--ALDYLLMAFAPTLWWLYLGR 98

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 145326108 346 VCCGL-GSARAVNRRYISDCVPLRIRMQAsAGFVSAS-ALGMACGPALAGLL-QIkfkfykftfnQSTLPGWVMAVAWLF 422
Cdd:cd17388   99 IIAGItGATGAVAAAYIADVTPPEQRARN-FGLLGAAfGLGFIAGPALGGLLgEI----------SPRAPFLVAAGLNGL 167

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 145326108 423 YLVWLCISFREPLRdtedGEKNNRNETTSAtdrvessrveeglrlpllitsgikpedeeecdeseespedshKPANSFie 502
Cdd:cd17388  168 NFLLGLFVLPESLP----PERRRAFRWRRA------------------------------------------NPFGSL-- 199

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 145326108 503 AYRLLTPSVKVQLLIYFMLKYSMEILLSESSVITSYYFSWTTSSVAIFLACLGLTVLPINILVGSYISNMFEDRQILLTS 582
Cdd:cd17388  200 RWLTGFPGLLGLLAVFFLIQLIGQVPATTWVLFTEDRFDWSPTMVGLSLAVFGLLHALFQAFVTGPIAKRLGEKRAILLG 279

                        330
                 ....*....|...
gi 145326108 583 EIIVFLG-ILFSF 594
Cdd:cd17388  280 LAADALGfLLLAF 292
SPX_PHO1_like cd14476
SPX domain of the plant protein PHOSPHATE1 (PHO1); This region has been named the SPX domain ...
 4-133 4.93e-06

SPX domain of the plant protein PHOSPHATE1 (PHO1); This region has been named the SPX domain after (Syg1, Pho81 and XPR1). The domain is found at the amino terminus of a variety of proteins. The PHO1 gene family conserved in plants is involved in a variety of processes, most notably the transport of inorganic phosphate from the root to the shoot of the plant and mediating the response to low levels of inorganic phosphate. More recently it has become evident that PHO1 gene families have diverged in various plants and may play roles in stress response as well as the stomatal response to abscisic acid.


Pssm-ID: 269897 [Multi-domain]  Cd Length: 139  Bit Score: 46.48  E-value: 4.93e-06
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 145326108   4 FGKYLQRKQIEEWSGYYINYKLMKKKVKQYAEQIQG--------GSQHPRHVLKDFSRmLDTQIETTVLFMLEQQGLLSG 75
Cdd:cd14476    2 FGKEFESQMVPEWQEAYVDYKQLKKDLKRIQKFRDEyettfleaAEEGGEYELVFFRR-LDDELNKVNKFYRSKVEEVLK 80

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|....*....
gi 145326108  76 RLAKLRESHDAILEQPDisrIFELREAYRDvgrdlLQLLK-FVELNAIGLRKILKKFDK 133
Cdd:cd14476   81 EAAALNKQMDALIAFRV---KVENPQFYRK-----LRLLKsYSFLNMLAFSKILKKYDK 131
SPX pfam03105
SPX domain; We have named this region the SPX domain after SYG1, Pho81 and XPR1. This 180 ...
 4-46 9.03e-06

SPX domain; We have named this region the SPX domain after SYG1, Pho81 and XPR1. This 180 residue long domain is found at the amino terminus of a variety of proteins. In the yeast protein SYG1, the N-terminus directly binds to the G-protein beta subunit and inhibits transduction of the mating pheromone signal. Similarly, the N-terminus of the human XPR1 protein binds directly to the beta subunit of the G-protein heterotrimer leading to increased production of cAMP. These findings suggest that all the members of this family are involved in G-protein associated signal transduction. The N-termini of several proteins involved in the regulation of phosphate transport, including the putative phosphate level sensors PHO81 from Saccharomyces cerevisiae and NUC-2 from Neurospora crassa, are also members of this family. The SPX domain of S. cerevisiae low-affinity phosphate transporters Pho87 and Pho90 auto-regulates uptake and prevents efflux. This SPX dependent inhibition is mediated by the physical interaction with Spl2 NUC-2 contains several ankyrin repeats pfam00023. Several members of this family are annotated as XPR1 proteins: the xenotropic and polytropic retrovirus receptor confers susceptibility to infection with murine xenotropic and polytropic leukaemia viruses (MLV). Infection by these retroviruses can inhibit XPR1-mediated cAMP signalling and result in cell toxicity and death. The similarity between SYG1, phosphate regulators and XPR1 sequences has been previously noted, as has the additional similarity to several predicted proteins, of unknown function, from Drosophila melanogaster, Arabidopsis thaliana, Caenorhabditis elegans, Schizosaccharomyces pombe, and Saccharomyces cerevisiae, and many other diverse organizms. In addition, given the similarities between XPR1 and SYG1 and phosphate regulatory proteins, it has been proposed that XPR1 might be involved in G-protein associated signal transduction and may itself function as a phosphate sensor.


Pssm-ID: 460807 [Multi-domain]  Cd Length: 339  Bit Score: 48.33  E-value: 9.03e-06
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|....*
gi 145326108    4 FGKYLQRKQIEEWSGYYINYKLMKKKVK--QYAEQIQGGSQHPRH 46
Cdd:pfam03105   3 FGKELEENLVPEWRDAYLDYKQLKKLIKkiQRELESTPPSSSPSS 47
Sugar_tr pfam00083
Sugar (and other) transporter;
260-351 7.17e-05

Sugar (and other) transporter;


Pssm-ID: 395036 [Multi-domain]  Cd Length: 452  Bit Score: 45.73  E-value: 7.17e-05
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 145326108  260 YMVNTYIIVPTADDYSMSLGAAATVCGVVIGSMAVAQVFSSVYFSAWSNK-SYFKPLVFSSIALFIGNLMYALAYDANSI 338
Cdd:pfam00083  23 LTLIDFFKNFGLSKSVSSLAALSVLSGLIVSIFSVGCFIGSLFAGKLGDRfGRKKSLLIANVLFVIGAVLQGAAKGKWSV 102

                          90
                  ....*....|...
gi 145326108  339 ALLLLGRVCCGLG 351
Cdd:pfam00083 103 YQLIVGRVLVGIG 115
UhpC COG2271
Sugar phosphate permease [Carbohydrate transport and metabolism];
250-431 8.22e-05

Sugar phosphate permease [Carbohydrate transport and metabolism];


Pssm-ID: 441872 [Multi-domain]  Cd Length: 363  Bit Score: 45.25  E-value: 8.22e-05
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 145326108 250 LLLNLGNTFLYMVNT---YIIVPTADDYSMSLGAAATVCGVVIGSMAVAQVFSSVYFSAWSNKsyfKPLVFSSIALFIGN 326
Cdd:COG2271   13 ALLFLAYFLNYLDRSnlsVAAPPLQADLGLSAAQLGLLASAFFLGYALGQIPAGLLADRFGRR---RVLAIGLLLWGLAT 89

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 145326108 327 LMYALAydaNSIALLLLGRVCCGLGSARA--VNRRYISDCVPLRIRMQASAGFVSASALGMACGPALAGLLQIKFKfYKF 404
Cdd:COG2271   90 LLFGFA---TSFWQLLLLRFLLGLGEAGFfpAALKLIAEWFPPKERGRALGIFNAGGPLGGALAPPLLGWLLAAFG-WRA 165

                        170       180
                 ....*....|....*....|....*....
gi 145326108 405 TFNQSTLPGWVMAVA--WLFYLVWLCISF 431
Cdd:COG2271  166 AFLILGLPGLLLALLrfWLLALAYFLVYF 194
MFS cd06174
Major Facilitator Superfamily; The Major Facilitator Superfamily (MFS) is a large and diverse ...
 258-442 9.14e-05

Major Facilitator Superfamily; The Major Facilitator Superfamily (MFS) is a large and diverse group of secondary transporters that includes uniporters, symporters, and antiporters. MFS proteins facilitate the transport across cytoplasmic or internal membranes of a variety of substrates including ions, sugar phosphates, drugs, neurotransmitters, nucleosides, amino acids, and peptides. They do so using the electrochemical potential of the transported substrates. Uniporters transport a single substrate, while symporters and antiporters transport two substrates in the same or in opposite directions, respectively, across membranes. MFS proteins are typically 400 to 600 amino acids in length, and the majority contain 12 transmembrane alpha helices (TMs) connected by hydrophilic loops. The N- and C-terminal halves of these proteins display weak similarity and may be the result of a gene duplication/fusion event. Based on kinetic studies and the structures of a few bacterial superfamily members, GlpT (glycerol-3-phosphate transporter), LacY (lactose permease), and EmrD (multidrug transporter), MFS proteins are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement. Bacterial members function primarily for nutrient uptake, and as drug-efflux pumps to confer antibiotic resistance. Some MFS proteins have medical significance in humans such as the glucose transporter Glut4, which is impaired in type II diabetes, and glucose-6-phosphate transporter (G6PT), which causes glycogen storage disease when mutated.


Pssm-ID: 349949 [Multi-domain]  Cd Length: 378  Bit Score: 45.50  E-value: 9.14e-05
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 145326108 258 FLYMVNTYIIVPTADDYSMSLGAAATVCGVVIGSMAVAQVFSSVYFSAWSNKSYFKPLVFSSIALFIgnLMYALAYDANS 337
Cdd:cd06174    6 FLTGLARGLISPLLPALLQSFGLSASQLGLLFALFSLGYALLQPLAGLLADRFGRRPVLLLGLLLFA--LGALLFAFAPS 83

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 145326108 338 IALLLLGRVCCGLGSA---RAVNrRYISDCVPLRIRMQASAGFVSASALGMACGPALAGLLqikfkFYKFTFNQSTLPGW 414
Cdd:cd06174   84 FWLLLLGRFLLGLGSGlidPAVL-ALIADLFPERERGRALGLLQAFGSVGGILGPLLGGIL-----ASSLGFGWRAVFLI 157

                        170       180
                 ....*....|....*....|....*...
gi 145326108 415 VMAVAWLFYLVWLCISFREPLRDTEDGE 442
Cdd:cd06174  158 AAALALLAAILLLLVVPDPPESARAKNE 185
MFS_NepI_like cd17324
Purine ribonucleoside efflux pump NepI and similar transporters of the Major Facilitator ...
 279-395 1.77e-04

Purine ribonucleoside efflux pump NepI and similar transporters of the Major Facilitator Superfamily; This family is composed of purine efflux pumps such as Escherichia coli NepI and Bacillus subtilis PbuE, sugar efflux transporters such as Corynebacterium glutamicum arabinose efflux permease, multidrug resistance (MDR) transporters such as Streptomyces lividans chloramphenicol resistance protein (CmlR), and similar proteins. NepI and PbuE are involved in the efflux of purine ribonucleosides such as guanosine, adenosine and inosine, as well as purine bases like guanine, adenine, and hypoxanthine, and purine base analogs. They play a role in the maintenance of cellular purine base pools, as well as in protecting the cells and conferring resistance against toxic purine base analogs such as 6-mercaptopurine. MDR transporters are drug/H+ antiporters (DHA) that mediate the efflux of a variety of drugs and toxic compounds, and confer resistance to these compounds. The NepI-like family belongs to the Major Facilitator Superfamily (MFS) of membrane transport proteins, which are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.


Pssm-ID: 340882 [Multi-domain]  Cd Length: 370  Bit Score: 44.46  E-value: 1.77e-04
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 145326108 279 GAAATVCGVVIGSMAVAQVFSSVYFSAWSNKSYFKPLVFSSIALFIgnLMYALAYDANSIALLLLGRVCCGLG--SARAV 356
Cdd:cd17324  230 GFSSSAIIGLLLLFGVAGVVGSPLAGRLADRGGRRALLIALLLLAA--ALLLLTLLGPSPLLLLVGLVLWGLGffAAHSA 307

                         90       100       110
                 ....*....|....*....|....*....|....*....
gi 145326108 357 NRRYISDCVPlRIRMQASAGFVSASALGMACGPALAGLL 395
Cdd:cd17324  308 LQTRVLRLAP-EARGLASSLYLAAYNLGGALGAALGGLV 345
MFS cd06174
Major Facilitator Superfamily; The Major Facilitator Superfamily (MFS) is a large and diverse ...
 254-425 2.77e-04

Major Facilitator Superfamily; The Major Facilitator Superfamily (MFS) is a large and diverse group of secondary transporters that includes uniporters, symporters, and antiporters. MFS proteins facilitate the transport across cytoplasmic or internal membranes of a variety of substrates including ions, sugar phosphates, drugs, neurotransmitters, nucleosides, amino acids, and peptides. They do so using the electrochemical potential of the transported substrates. Uniporters transport a single substrate, while symporters and antiporters transport two substrates in the same or in opposite directions, respectively, across membranes. MFS proteins are typically 400 to 600 amino acids in length, and the majority contain 12 transmembrane alpha helices (TMs) connected by hydrophilic loops. The N- and C-terminal halves of these proteins display weak similarity and may be the result of a gene duplication/fusion event. Based on kinetic studies and the structures of a few bacterial superfamily members, GlpT (glycerol-3-phosphate transporter), LacY (lactose permease), and EmrD (multidrug transporter), MFS proteins are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement. Bacterial members function primarily for nutrient uptake, and as drug-efflux pumps to confer antibiotic resistance. Some MFS proteins have medical significance in humans such as the glucose transporter Glut4, which is impaired in type II diabetes, and glucose-6-phosphate transporter (G6PT), which causes glycogen storage disease when mutated.


Pssm-ID: 349949 [Multi-domain]  Cd Length: 378  Bit Score: 43.95  E-value: 2.77e-04
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 145326108 254 LGNTFLYMVNTYIIVPTADDYSMSLGAAatvcGVVIGSMAVAQVFSSVYFSAWSNKSYF-KPLVFSSIALFIGNLMYALA 332
Cdd:cd06174  214 LVNLAYYSFSTLLPLFLLDLGGLSVAVA----GLLLSLFGLAGALGSLLLGLLSDRLIGrKPLLLIGLLLMALGLALLLL 289

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 145326108 333 YDANSIALLLLGRVCCGLGSARAVNRRYISDCVPLRIRMQASAGFVSASALGMACGPALAGLLQIKFKFYKFTFnqstlp 412
Cdd:cd06174  290 APSLLLLLLLLLLLGFGLGGLLPLSFALIAELFPPEIRGTAFGLLNTFGFLGGAIGPLLAGFLLAATFGLTGAF------ 363

                        170
                 ....*....|...
gi 145326108 413 gWVMAVAWLFYLV 425
Cdd:cd06174  364 -LVLAVLLLLAAI 375
SPX_XPR1_like cd14477
SPX domain of the xenotropic and polytropic retrovirus receptor 1 (XPR1) and related proteins; ...
 4-133 2.90e-04

SPX domain of the xenotropic and polytropic retrovirus receptor 1 (XPR1) and related proteins; This region has been named the SPX domain after (Syg1, Pho81 and XPR1). The domain is found at the amino terminus of a variety of proteins. The N-terminus of the human XPR1 protein (xenotropic and polytropic retrovirus receptor 1) binds directly to the beta subunit of the G-protein heterotrimer leading to increased production of cAMP. These findings suggest that all members of this family are involved in G-protein associated signal transduction. Several members of this family are annotated as XPR1 proteins: the xenotropic and polytropic retrovirus receptor confers susceptibility to infection with xenotropic and polytropic murine leukaemia viruses (MLV). Infection by these retroviruses can inhibit XPR1-mediated cAMP signaling and result in cell toxicity and death. Similarity between Syg1, phosphate regulators and XPR1 sequences has been previously noted, as has the additional similarity to several predicted proteins, of unknown function, from Drosophila melanogaster, Arabidopsis thaliana, Caenorhabditis elegans, Schizosaccharomyces pombe, and Saccharomyces cerevisiae, and many other diverse organisms.


Pssm-ID: 269898 [Multi-domain]  Cd Length: 161  Bit Score: 41.89  E-value: 2.90e-04
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 145326108   4 FGKYLQRKQIEEWSGYYINYKLMKKKVKQYAEQ---IQGGSQHP--RHVLK---DFSRMLDTQIETTVLFMLEQQGLLSG 75
Cdd:cd14477    2 FGEHLSAHITPEWRKQYINYEELKAMLYAAVEQapsPEVTDEDVvkRYFAKfeeEFFQECDKELAKVNTFFSEKLAEAQR 81

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 145326108  76 RLAKLRESHDAILEQPD--------ISRIFELREAYRDVGRDLLQL-LKFVE-------------LNAIGLRKILKKFDK 133
Cdd:cd14477   82 KFATLKNELLSSLEAQGesgaasslIRRVFALLRKERVKPRKLRDLkLAFSEfylslillqnyqnLNFTGFRKILKKHDK 161
SPX_BAH1-like cd14482
SPX domain of the E3 ubiquitin-protein ligase BAH1/NLA and similar proteins; This region has ...
 79-133 9.91e-04

SPX domain of the E3 ubiquitin-protein ligase BAH1/NLA and similar proteins; This region has been named the SPX domain after (Syg1, Pho81 and XPR1). The domain is found at the amino terminus of a variety of proteins. BAH1 (benzoic acid hypersensitive 1) appears to function as an E3 ubiquitin ligase; the protein contains an SPX and a RING finger domain. It has been suggested that BAH1/NLA is involved in the regulation of plant immune responses, probably via a pathway of salicylic acid biosynthesis that includes benzoic acid as an intermediate.


Pssm-ID: 269903  Cd Length: 156  Bit Score: 40.41  E-value: 9.91e-04
                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*
gi 145326108  79 KLRESHDAILEQpdisrifelreayrdvGRDLLQllkFVELNAIGLRKILKKFDK 133
Cdd:cd14482  113 CFKDDHQALIQE----------------GRDLVN---YATMNAIAIRKILKKYDK 148
COG5408 COG5408
SPX domain-containing protein [Signal transduction mechanisms];
109-152 1.13e-03

SPX domain-containing protein [Signal transduction mechanisms];


Pssm-ID: 227695 [Multi-domain]  Cd Length: 296  Bit Score: 41.74  E-value: 1.13e-03
                         10        20        30        40
                 ....*....|....*....|....*....|....*....|....
gi 145326108 109 DLLQLLKFVELNAIGLRKILKKFDKRFGYRFADYYVKtRANHPY 152
Cdd:COG5408  253 QLHQLKSFIELNYTGFSKITKKYDKTLHQNLRHEYMS-RSVNEY 295
AraJ COG2814
Predicted arabinose efflux permease AraJ, MFS family [Carbohydrate transport and metabolism];
254-386 1.19e-03

Predicted arabinose efflux permease AraJ, MFS family [Carbohydrate transport and metabolism];


Pssm-ID: 442063 [Multi-domain]  Cd Length: 348  Bit Score: 41.88  E-value: 1.19e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 145326108 254 LGNTFLYMVNTYIIVPTADDYSMSLGAAatvcGVVIGSMAVAQVFSSVYFSAWSNKSYFKPLVFSSIALFIgnLMYALAY 333
Cdd:COG2814  218 LLGFGFFALFTYLPLYLQEVLGLSASAA----GLLLALFGLGGVLGALLAGRLADRFGRRRLLLIGLLLLA--LGLLLLA 291

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|....*
gi 145326108 334 DANSIALLLLGRVCCGLG--SARAVNRRYISDCVPLRIRMQASAGFVSASALGMA 386
Cdd:COG2814  292 LAGSLWLLLLALFLLGFGfgLLFPLLQALVAELAPPEARGRASGLYNSAFFLGGP 346
MFS_arabinose_efflux_permease_like cd17473
Putative arabinose efflux permease family transporters of the Major Facilitator Superfamily; ...
 249-427 1.32e-03

Putative arabinose efflux permease family transporters of the Major Facilitator Superfamily; This family includes a group of putative arabinose efflux permease family transporters, such as alpha proteobacterium quinolone resistance protein NorA (characterized Staphylococcus aureus Quinolone resistance protein NorA belongs to a different group), Desulfovibrio dechloracetivorans bacillibactin exporter, Vibrio aerogenes antiseptic resistance protein. The biological function of those transporters remain unclear. They belong to the Major Facilitator Superfamily (MFS) of membrane transport proteins, which are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.


Pssm-ID: 341026 [Multi-domain]  Cd Length: 374  Bit Score: 41.79  E-value: 1.32e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 145326108 249 SLLLNLGNTFLYMVNTYIIVPTADDYSMSLG-AAATVCGVVIGSMAVAQVFSSVYFSawsnksYFKPLVFSSIALFIGNL 327
Cdd:cd17473  198 RVLPIYFLAFLAMLLFYVIPIQLPFLLQERGgGSAALIGLALAIASLAGAIGALLFG------RLKRRLGKRRLLAIGFA 271

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 145326108 328 MYALAY----DANSIALLLLGRVCCGLGSA---RAVNRRyISDCVPLRIRMQASAGFVSASALGMACGPALAGLLQIKFK 400
Cdd:cd17473  272 LMALGFlllaLASGLWVVLLGAILAGLGFGlllPTLNSW-AMSLVPPELRGRAMGIVTSAFFLGQFLSPLVLGPLVNLTG 350

                        170       180
                 ....*....|....*....|....*..
gi 145326108 401 FYKFTFnqsTLPGWVMAVAWLFYLVWL 427
Cdd:cd17473  351 GLSGAF---LILGVLALVLAIVLLLLR 374
MFS_GLUT_like cd17315
Glucose transporters (GLUTs) and other similar sugar transporters of the Major Facilitator ...
 254-422 2.36e-03

Glucose transporters (GLUTs) and other similar sugar transporters of the Major Facilitator Superfamily; This family is composed of glucose transporters (GLUTs) and other sugar transporters including fungal hexose transporters (HXT), bacterial xylose transporter (XylE), plant sugar transport proteins (STP) and polyol transporters (PLT), H(+)-myo-inositol cotransporter (HMIT), and similar proteins. GLUTs, also called Solute carrier family 2, facilitated glucose transporters (SLC2A), are a family of proteins that facilitate the transport of hexoses such as glucose and fructose. There are fourteen GLUTs found in humans; they display different substrate specificities and tissue expression. They have been categorized into three classes based on sequence similarity: Class 1 (GLUTs 1-4, 14); Class 2 (GLUTs 5, 7, 9, and 11); and Class 3 (GLUTs 6, 8, 10, 12, and HMIT). GLUT proteins are comprised of about 500 amino acid residues, possess a single N-linked oligosaccharide, and have 12 transmembrane segments. The GLUT-like family belongs to the Major Facilitator Superfamily (MFS) of membrane transport proteins, which are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.


Pssm-ID: 340873 [Multi-domain]  Cd Length: 365  Bit Score: 40.64  E-value: 2.36e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 145326108 254 LGNtFLYMVNTYIIVPTADDYSMSLGAA--ATVCGVVIGSMAVAQVFSSVyFSAWSNKSY--FKPLVFSSIALFIGNLMY 329
Cdd:cd17315    4 LGG-LLFGYDLGVINGALLYIAKDLGFGlsTSLQGLVVSSLLLGAAIGSL-FGGPLADRFgrRKSLLIAAVLYVIGALLS 81

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 145326108 330 ALAydaNSIALLLLGRVCCGL--GSARAVNRRYISDCVPLRIR--------MQASAGFVSASALGMACGPALAGLLQIKF 399
Cdd:cd17315   82 ALA---PNVWVLIVGRFLLGLgvGLASVLVPLYISEIAPAKIRgalgtlnqLMITFGILLAYLLGLALSLSPPGWWRLMF 158

                        170       180
                 ....*....|....*....|...
gi 145326108 400 KFykftfnqSTLPGWVMAVAWLF 422
Cdd:cd17315  159 AL-------AAVPALLQLLLMFF 174
MFS_YfcJ_like cd17489
Escherichia coli YfcJ, YhhS, and similar transporters of the Major Facilitator Superfamily; ...
 274-395 4.73e-03

Escherichia coli YfcJ, YhhS, and similar transporters of the Major Facilitator Superfamily; This subfamily is composed of Escherichia coli membrane proteins, YfcJ and YhhS, Bacillus subtilis uncharacterized MFS-type transporter YwoG, and similar proteins. YfcJ and YhhS are putative arabinose efflux transporters. YhhS has been implicated glyphosate resistance. YfcJ-like arabinose efflux transporters belong to the bacterial MdtG-like and eukaryotic solute carrier 18 (SLC18) family of the Major Facilitator Superfamily (MFS) of transporters. MFS proteins are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.


Pssm-ID: 341042 [Multi-domain]  Cd Length: 367  Bit Score: 39.88  E-value: 4.73e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 145326108 274 YSMSLGAAATvcGVVIGSMAVAQVFSSVYFSAWSNKsyFKPLVFSSIALFIGNLMYALAYDANSIALLLLGRVCCGLGSA 353
Cdd:cd17489  220 YAAERGISNA--GLFFTVYAIALLLSRPFSGKLSDR--KGPKTVIIPGLLLLALGLLLLSFAGSPWMLLLAAVLYGLGFG 295

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....
gi 145326108 354 RAVN--RRYISDCVPLRIRMQASAGFVSASALGMACGPALAGLL 395
Cdd:cd17489  296 LLFPalQALAVDLVPPHRRGAANGTFFIFFDLGIGLGGLLLGLV 339
MFS_Set cd17471
Sugar efflux transporter (Set) family of the Major Facilitator Superfamily of transporters; ...
 278-432 4.80e-03

Sugar efflux transporter (Set) family of the Major Facilitator Superfamily of transporters; This family is composed of sugar transporters such as Escherichia coli Sugar efflux transporter SetA, SetB, SetC and other sugar transporters. SetA, SetB, and SetC are involved in the efflux of sugars such as lactose, glucose, IPTG, and substituted glucosides or galactosides. They may be involved in the detoxification of non-metabolizable sugar analogs. The Set family belongs to the Major Facilitator Superfamily (MFS) of membrane transport proteins, which are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.


Pssm-ID: 341024 [Multi-domain]  Cd Length: 371  Bit Score: 39.84  E-value: 4.80e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 145326108 278 LGAAATVCGVVIGSMAVAQVFSSVYFSAWSNK--SYFKPLVFSSIALFIGNLMYALAydANSIALLLLGRVCCGLGSAR- 354
Cdd:cd17471   27 LGASPLQIGIFMTLSALSGIVVSQWLGRLSDRggDRKPLILLALLAGALGYLLFAFL--RNYWVLLLVGVVLLSLGAAAf 104

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 145326108 355 ----AVNRRYISDCVPLRIRMqASAGFVSASALGMACGPALAGLLQIKFKFYkftfnqstlpgWVMAVAWLFYLVWLCIS 430
Cdd:cd17471  105 pqlfALAREYADRSSGRDATL-FNSVLRAAFSLAWVIGPPLAFLLLDVLGFT-----------GLFLLAALLYLVVALLV 172


                 ..
gi 145326108 431 FR 432
Cdd:cd17471  173 LF 174
UhpC COG2271
Sugar phosphate permease [Carbohydrate transport and metabolism];
247-395 7.62e-03

Sugar phosphate permease [Carbohydrate transport and metabolism];


Pssm-ID: 441872 [Multi-domain]  Cd Length: 363  Bit Score: 39.08  E-value: 7.62e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 145326108 247 FNSLLLNLGNTFLYMVNTYIIV--PT--ADDYSMSLGAAATVCGVVIGSMAVAQVFSSvYFSAWSNKSYFKPLVFSSIAL 322
Cdd:COG2271  180 LRFWLLALAYFLVYFALYGFLTwlPTylVEVRGLSLAQAGLLLSLPFLAGIVGSLLGG-WLSDRLGRRRKLVLAIGLLLA 258

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 145326108 323 FIGnLMYALAYDANSIALLLLGRVCCGLGSARAVNRRYISDCVPLRIRMQASAGFVSASALGMACGPALAGLL 395
Cdd:COG2271  259 ALA-LLLLALLPSPALAIALLFLAGFGLGGAFGLLWALAAELFPKKARGTASGLVNTFGFLGGALGPLLVGYL 330
MelB COG2211
Na+/melibiose symporter or related transporter [Carbohydrate transport and metabolism];
250-425 8.08e-03

Na+/melibiose symporter or related transporter [Carbohydrate transport and metabolism];


Pssm-ID: 441813 [Multi-domain]  Cd Length: 447  Bit Score: 39.12  E-value: 8.08e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 145326108 250 LLLNLGNTFLYMVNTYIIVptaddYSMSLGAAATvcGVVIGSMAVAQVFSSVYFSAWSNKSYFKPLVFSSIALFIGNLMY 329
Cdd:COG2211  238 LLFFLALALVAALLLYYFK-----YVLGLSAALV--GLLLALYFLAALLGAPLWPRLAKRFGKKKAFIIGLLLAALGLLL 310

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 145326108 330 ALAYDANSIALLLLGRVCCGLGSA--RAVNRRYISDCVPL-------RIRMQASAGFVSASALGMACGPALAGLLqikFK 400
Cdd:COG2211  311 LFFLGPGNLWLLLVLAALAGIGLGaiLVLPWAMLADVVDYdewktgrRREGLYFGIFTFAIKLGQALAGALAGLL---LA 387

                        170       180
                 ....*....|....*....|....*
gi 145326108 401 FYKFTFNQSTLPGWVMAVAWLFYLV 425
Cdd:COG2211  388 LFGYVAGAAQSPSALTGIRLLFFLL 412
CynX COG2807
Cyanate permease [Inorganic ion transport and metabolism];
254-427 1.00e-02

Cyanate permease [Inorganic ion transport and metabolism];


Pssm-ID: 442057 [Multi-domain]  Cd Length: 399  Bit Score: 38.70  E-value: 1.00e-02
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 145326108 254 LGNTFLYMVNTYIivPTaddYSMSLGAAATVCGVVIGSMAVAQVFSSVYFSAWSNK--SYFKPLVFSSIALFIGNLMYAL 331
Cdd:COG2807  226 LQSLLYYAVVAWL--PP---ILRDAGLSAATAGLLLSLFQLAGIPGSLLVPLLADRlgDRRPLLLLLGLLGLAGLLGLLL 300

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 145326108 332 AYDANS-IALLLLGrvcCGLGSARAVNRRYISDCVP-LRIRMQASAGFVSASALGMACGPALAGLLqikfkfykFTFNQS 409
Cdd:COG2807  301 APGSLPwLWAVLLG---LGQGGLFPLALTLIGLRARtPAEAAALSGMAQSVGYLLAALGPLLVGAL--------HDATGS 369

                        170
                 ....*....|....*...
gi 145326108 410 TLPGWVMAVAWLFYLVWL 427
Cdd:COG2807  370 WTAALLLLAALAVLMLVA 387
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
Help | Disclaimer | Write to the Help Desk
NCBI | NLM | NIH