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Conserved domains on  [gi|115430091|ref|NP_001068578|]
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OTU domain-containing protein 5-A [Danio rerio]

Protein Classification

OTU domain-containing protein 5( domain architecture ID 17784769)

OTU domain-containing protein 5 (OTUD5) acts as a ubiquitinating enzyme that functions as a negative regulator of the innate immune system

CATH:  1.10.10.1640|3.30.200.90|3.90.70.80
Gene Ontology:  GO:0004843
PubMed:  10664582|23827681|12704427|31884527
SCOP:  4004307

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
OTU_OTUD5-like cd22752
OTU (ovarian tumor) domain of OTU domain-containing protein 5 and similar proteins; OTU ...
 210-333 5.28e-88

OTU (ovarian tumor) domain of OTU domain-containing protein 5 and similar proteins; OTU domain-containing protein 5 (OTUD5), also called deubiquitinating enzyme A (DUBA), is a phosphorylation-dependent deubiquitinase (DUB)/ubiquitin thioesterase (EC 3.4.19.12) that can hydrolyze 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains, and may function as negative regulator of the innate immune system. It limits type I interferon production in macrophages and suppresses interleukin-17A production in T cells. OTUD5 also functions in an apoptotic signaling cascade by mediating the sequential activation of PDCD5 (programmed cell death 5) and p53 in response to genotoxic stress. In Drosophila, OTUD5/DUBA is essential for spermatogenesis. This subfamily also includes Arabidopsis thaliana OTU domain-containing protein 6, also called deubiquitinating enzyme OTU6 or otubain-like deubiquitinase 1 (OTLD1), which binds chromatin and has enzymatic histone deubiquitinase activity specific for the H2B histone. This subfamily belongs to the OTU family of cysteine proteases that use a conserved cysteine, histidine, and an aspartate, as the catalytic triad. It also includes plant OTU6.


:

Pssm-ID: 438589 [Multi-domain]  Cd Length: 124  Bit Score: 267.11  E-value: 5.28e-88
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 115430091 210 GFVIKKMKEDGACLFRAVADQVYGDQDMHEVVRKHCMDYLMKNADYFSNYVTEDFTTYINRKRKNNCHGNHIEMQAMAEM 289
Cdd:cd22752    1 GFIIKEMEEDGNCLFRAVADQVYGDQEMHDVVRKHCMDYMEKNRDYFSQFVTEDFEEYINRKRQDGVWGNHIEIQAMSEL 80

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....
gi 115430091 290 YNRPVEVYQSGTEPINTFHGIHQNNDEPIRVSYHRNIHYNSVVN 333
Cdd:cd22752   81 YNRPIEVYAYSTEPINTFHEASSSDNEPIRLSYHGNSHYNSIVD 124
DUF6264 super family cl45176
Family of unknown function (DUF6264); This family of putative integral membrane proteins is ...
 433-538 1.92e-03

Family of unknown function (DUF6264); This family of putative integral membrane proteins is functionally uncharacterized. This family of proteins is found in bacteria. Proteins in this family are typically between 179 and 218 amino acids in length.


The actual alignment was detected with superfamily member pfam19779:

Pssm-ID: 466182  Cd Length: 182  Bit Score: 39.56  E-value: 1.92e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 115430091  433 EEWNARSPRQRSSAPSPEIPDPAHSDTAAKPPSPAGALALSKPPSPCAPGPSNQACVGP--DRP------------TSSS 498
Cdd:pfam19779  10 PGWQRAPIGDPAAAAAAAPPAAPAPAAPAPPAAPAAPPAAPPPPGAPAPGAPAAARRARrwDRIatiallvfglfnVLSS 89

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|
gi 115430091  499 LVSLypaLGYRAIMQEMSpTAFGLTDWEDDEILASVLAVS 538
Cdd:pfam19779  90 IPSL---LDLPSTLQTLF-AQLGIDEFTPPAAARTWGIVA 125
 
Name Accession Description Interval E-value
OTU_OTUD5-like cd22752
OTU (ovarian tumor) domain of OTU domain-containing protein 5 and similar proteins; OTU ...
 210-333 5.28e-88

OTU (ovarian tumor) domain of OTU domain-containing protein 5 and similar proteins; OTU domain-containing protein 5 (OTUD5), also called deubiquitinating enzyme A (DUBA), is a phosphorylation-dependent deubiquitinase (DUB)/ubiquitin thioesterase (EC 3.4.19.12) that can hydrolyze 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains, and may function as negative regulator of the innate immune system. It limits type I interferon production in macrophages and suppresses interleukin-17A production in T cells. OTUD5 also functions in an apoptotic signaling cascade by mediating the sequential activation of PDCD5 (programmed cell death 5) and p53 in response to genotoxic stress. In Drosophila, OTUD5/DUBA is essential for spermatogenesis. This subfamily also includes Arabidopsis thaliana OTU domain-containing protein 6, also called deubiquitinating enzyme OTU6 or otubain-like deubiquitinase 1 (OTLD1), which binds chromatin and has enzymatic histone deubiquitinase activity specific for the H2B histone. This subfamily belongs to the OTU family of cysteine proteases that use a conserved cysteine, histidine, and an aspartate, as the catalytic triad. It also includes plant OTU6.


Pssm-ID: 438589 [Multi-domain]  Cd Length: 124  Bit Score: 267.11  E-value: 5.28e-88
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 115430091 210 GFVIKKMKEDGACLFRAVADQVYGDQDMHEVVRKHCMDYLMKNADYFSNYVTEDFTTYINRKRKNNCHGNHIEMQAMAEM 289
Cdd:cd22752    1 GFIIKEMEEDGNCLFRAVADQVYGDQEMHDVVRKHCMDYMEKNRDYFSQFVTEDFEEYINRKRQDGVWGNHIEIQAMSEL 80

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....
gi 115430091 290 YNRPVEVYQSGTEPINTFHGIHQNNDEPIRVSYHRNIHYNSVVN 333
Cdd:cd22752   81 YNRPIEVYAYSTEPINTFHEASSSDNEPIRLSYHGNSHYNSIVD 124
OTU pfam02338
OTU-like cysteine protease; This family is comprised of a group of predicted cysteine ...
 219-328 1.58e-18

OTU-like cysteine protease; This family is comprised of a group of predicted cysteine proteases, homologous to the Ovarian Tumour (OTU) gene in Drosophila. Members include proteins from eukaryotes, viruses and pathogenic bacterium. The conserved cysteine and histidine, and possibly the aspartate, represent the catalytic residues in this putative group of proteases.


Pssm-ID: 426728 [Multi-domain]  Cd Length: 127  Bit Score: 81.73  E-value: 1.58e-18
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 115430091  219 DGACLFRAVADQVYGDQDM-----HEVVRKHCMDYLMKNADYFSNYVTEDFTTYINRKRKNNCHGNHIEMQAMAEMYNRP 293
Cdd:pfam02338   3 DGNCLYRSISHQLWGVHDVlrkmlVQELRETLAEYMREHKEEFEPFLEDDETGDIIEIEQTGAWGGEIEIFALAHILRRP 82

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|
gi 115430091  294 VEVYQSGTEPIN---TFHGIHQNN--DEPIRVSYHRNIHY 328
Cdd:pfam02338  83 IIVYKSEGGEELgglKEYGIYLPLgwDPSLCLVYPRHLYY 122
DUF6264 pfam19779
Family of unknown function (DUF6264); This family of putative integral membrane proteins is ...
 433-538 1.92e-03

Family of unknown function (DUF6264); This family of putative integral membrane proteins is functionally uncharacterized. This family of proteins is found in bacteria. Proteins in this family are typically between 179 and 218 amino acids in length.


Pssm-ID: 466182  Cd Length: 182  Bit Score: 39.56  E-value: 1.92e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 115430091  433 EEWNARSPRQRSSAPSPEIPDPAHSDTAAKPPSPAGALALSKPPSPCAPGPSNQACVGP--DRP------------TSSS 498
Cdd:pfam19779  10 PGWQRAPIGDPAAAAAAAPPAAPAPAAPAPPAAPAAPPAAPPPPGAPAPGAPAAARRARrwDRIatiallvfglfnVLSS 89

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|
gi 115430091  499 LVSLypaLGYRAIMQEMSpTAFGLTDWEDDEILASVLAVS 538
Cdd:pfam19779  90 IPSL---LDLPSTLQTLF-AQLGIDEFTPPAAARTWGIVA 125
 
Name Accession Description Interval E-value
OTU_OTUD5-like cd22752
OTU (ovarian tumor) domain of OTU domain-containing protein 5 and similar proteins; OTU ...
 210-333 5.28e-88

OTU (ovarian tumor) domain of OTU domain-containing protein 5 and similar proteins; OTU domain-containing protein 5 (OTUD5), also called deubiquitinating enzyme A (DUBA), is a phosphorylation-dependent deubiquitinase (DUB)/ubiquitin thioesterase (EC 3.4.19.12) that can hydrolyze 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains, and may function as negative regulator of the innate immune system. It limits type I interferon production in macrophages and suppresses interleukin-17A production in T cells. OTUD5 also functions in an apoptotic signaling cascade by mediating the sequential activation of PDCD5 (programmed cell death 5) and p53 in response to genotoxic stress. In Drosophila, OTUD5/DUBA is essential for spermatogenesis. This subfamily also includes Arabidopsis thaliana OTU domain-containing protein 6, also called deubiquitinating enzyme OTU6 or otubain-like deubiquitinase 1 (OTLD1), which binds chromatin and has enzymatic histone deubiquitinase activity specific for the H2B histone. This subfamily belongs to the OTU family of cysteine proteases that use a conserved cysteine, histidine, and an aspartate, as the catalytic triad. It also includes plant OTU6.


Pssm-ID: 438589 [Multi-domain]  Cd Length: 124  Bit Score: 267.11  E-value: 5.28e-88
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 115430091 210 GFVIKKMKEDGACLFRAVADQVYGDQDMHEVVRKHCMDYLMKNADYFSNYVTEDFTTYINRKRKNNCHGNHIEMQAMAEM 289
Cdd:cd22752    1 GFIIKEMEEDGNCLFRAVADQVYGDQEMHDVVRKHCMDYMEKNRDYFSQFVTEDFEEYINRKRQDGVWGNHIEIQAMSEL 80

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....
gi 115430091 290 YNRPVEVYQSGTEPINTFHGIHQNNDEPIRVSYHRNIHYNSVVN 333
Cdd:cd22752   81 YNRPIEVYAYSTEPINTFHEASSSDNEPIRLSYHGNSHYNSIVD 124
OTU_plant_OTU6-like cd22796
OTU (ovarian tumor) domain of deubiquitinating enzyme OTU6 from plants and similar proteins; ...
 208-333 6.37e-68

OTU (ovarian tumor) domain of deubiquitinating enzyme OTU6 from plants and similar proteins; Deubiquitinating enzyme OTU6, also called OTU domain-containing protein 6 or otubain-like deubiquitinase 1 (OTLD1), is a deubiquitinase (DUB) or ubiquitin thiolesterase (EC 3.4.19.12) that catalyzes the thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin, a small regulatory protein that can be conjugated to a large range of target proteins. Protein ubiquitination is a post-translational modification of mostly Lys residues that regulates many cellular processes, including protein degradation, intracellular trafficking, cell signaling, autophagy, transcription, translation, and the DNA damage response. OTU6 binds chromatin and has enzymatic histone deubiquitinase activity specific for the H2B histone. OTU6 belongs to the OTU family of cysteine proteases that use a conserved cysteine, histidine, and an aspartate, as the catalytic triad.


Pssm-ID: 438617 [Multi-domain]  Cd Length: 128  Bit Score: 215.37  E-value: 6.37e-68
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 115430091 208 KKGFVIKKMKEDGACLFRAVADQVYGDQDMHEVVRKHCMDYLMKNADYFSNYVTEDFTTYINRKRKNNCHGNHIEMQAMA 287
Cdd:cd22796    2 KKGLEIRRMDGDGNCLFRAVADQVYGDQEMHDEVREMCMDYMEKERDHFSQFVTEDFTQYVKRKRRDRVFGNNLEIQAMS 81

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....*..
gi 115430091 288 EMYNRPVEVYQ-SGTEPINTFHGIHQNNDEPIRVSYHRNIHYNSVVN 333
Cdd:cd22796   82 EIYNRPIEVYSySNGEPINIFHGSYEGDDPPIRLSYHDGNHYNSIID 128
OTU cd22744
OTU (ovarian tumor) domain family; The OTU family of cysteine proteases use a conserved ...
 212-331 1.99e-36

OTU (ovarian tumor) domain family; The OTU family of cysteine proteases use a conserved cysteine and histidine, and in most cases an aspartate, as the catalytic triad. OTU domains typically function as deubiquitinases (DUBs)/ubiquitin thiolesterases (EC 3.4.19.12) that catalyze the thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin, a small regulatory protein that can be conjugated to a large range of target proteins. Protein ubiquitination is a post-translational modification of mostly Lys residues that regulates many cellular processes, including protein degradation, intracellular trafficking, cell signaling, autophagy, transcription, translation, and the DNA damage response. These DUBs may play important regulatory roles at the level of protein turnover by preventing degradation.


Pssm-ID: 438581 [Multi-domain]  Cd Length: 128  Bit Score: 131.79  E-value: 1.99e-36
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 115430091 212 VIKKMKEDGACLFRAVADQVYGDQDMHEVVRKHCMDYLMKNADYFSN------YVTEDFTTYINRKRKNNCHGNHIEMQA 285
Cdd:cd22744    1 RVVDVPGDGNCLFRALAHALYGDQESHRELRQEVVDYLRENPDLYEPaeladeDDGEDFDEYLQRMRKPGTWGGELELQA 80

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....*...
gi 115430091 286 MAEMYNRPVEVYQS--GTEPINTFHGIHQNNDEPIRVSYHRNIHYNSV 331
Cdd:cd22744   81 LANALNVPIVVYSEdgGFLPVSVFGPGPGPSGRPIHLLYTGGNHYDAL 128
OTU_plant_OTU7-like cd22771
OTU (ovarian tumor) domain of Arabidopsis thaliana deubiquitinating enzyme OTU7 and similar ...
 210-331 1.85e-33

OTU (ovarian tumor) domain of Arabidopsis thaliana deubiquitinating enzyme OTU7 and similar proteins; Arabidopsis thaliana deubiquitinating enzyme OTU7, also called OTU domain-containing protein 7, is a deubiquitinase (DUB)/ubiquitin thioesterase (EC 3.4.19.12) that shows a preference for 'Lys-63' over 'Lys-48' over 'Met-1'-linked ubiquitin (UB) tetramers as substrates. DUBs catalyze the thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin, a small regulatory protein that can be conjugated to a large range of target proteins. Protein ubiquitination is a post-translational modification of mostly Lys residues that regulates many cellular processes, including protein degradation, intracellular trafficking, cell signaling, autophagy, transcription, translation, and the DNA damage response. These DUBs may play important regulatory roles at the level of protein turnover by preventing degradation. OTU7 belongs to the OTU family of cysteine proteases that use a conserved cysteine, histidine, and an aspartate, as the catalytic triad.


Pssm-ID: 438608 [Multi-domain]  Cd Length: 124  Bit Score: 123.43  E-value: 1.85e-33
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 115430091 210 GFVIKKMKEDGACLFRAVADQVYGDQDMHEVVRKHCMDYLMKNADYFSNYVT--EDFTTYINRKRKNNCHGNHIEMQAMA 287
Cdd:cd22771    1 GLRIRDVEGDGNCLFRALADQLYGDEERHAELRKKVVDYMEAHEEDFEPFFEddETFEDYVSRMREDGTWGGNLELQAAS 80

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....*...
gi 115430091 288 EMYNRPVEVYQSGtEPINTFhgihQNNDEP----IRVSYHRNIHYNSV 331
Cdd:cd22771   81 LVYRVNIVVHQLG-QPRWEI----ENFPDKgartIHLSYHDGEHYNSV 123
OTU_OTUD6-like cd22748
OTU (ovarian tumor) domain of OTU domain-containing proteins 6A, 6B, and similar proteins; ...
 207-332 8.31e-30

OTU (ovarian tumor) domain of OTU domain-containing proteins 6A, 6B, and similar proteins; This subfamily is composed of mammalian OTU domain-containing protein 6A (OTUD6A, also called DUBA-2, vertebrate OTU domain-containing protein 6B (OTUD6B, also called DUBA-5), fungal OTU domain-containing protein 2 (OTU2), and similar proteins. OTUD6A, OTUD6B, and Schizosaccharomyces pombe OTU2 are deubiquitinating enzymes/ubiquitinyl hydrolases (EC 3.4.19.12). OTUD6A hydrolyzes 'Lys-27'-, 'Lys-29'-, and 'Lys-33'-linked polyubiquitin chains, and may also be able to hydrolyze 'Lys-11'-linked ubiquitin chains. It deubiquitylates and stabilizes dynamin-related protein 1 (Drp1), a cytosolic protein responsible for mitochondrial fission and is essential in the initiation and development of several human diseases including cancer, thereby facilitating tumorigenesis. OTUD6B is a functional deubiquitinase in in vitro enzyme assays. It may play a role in the ubiquitin-dependent regulation of protein synthesis downstream of mTORC1, and may modify the ubiquitination of the protein synthesis initiation complex to repress translation. Biallelic variants in OTUD6B cause an intellectual disability syndrome that is associated with seizures and dysmorphic features. This subfamily belongs to the OTU family of cysteine proteases that use a conserved cysteine, histidine, and an aspartate, as the catalytic triad.


Pssm-ID: 438585 [Multi-domain]  Cd Length: 144  Bit Score: 114.20  E-value: 8.31e-30
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 115430091 207 EKKGFVIKKMKEDGACLFRAVADQV---YGDQDMHEV--VRKHCMDYLMKNADYFSNYVT---------EDFTTYINRKR 272
Cdd:cd22748    2 KPLGLRIKEIPPDGHCLYRAIADQLklrGGSEEPYSYkeLRKLAADYMRAHRDDFLPFLTnddgdlmteEEFEEYCDKIE 81

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 115430091 273 KNNCHGNHIEMQAMAEMYNRPVEVYQSGTEPINtfHGIHQNNDEPIRVSYHRNI-----HYNSVV 332
Cdd:cd22748   82 NTAEWGGQLELRALSKALKRPIHVYQAGSPPLV--IGEEFDSGEPLRLSYHRHAyglgeHYNSVV 144
OTU_ALG13-like cd22753
OTU (ovarian tumor) domain of bifunctional UDP-N-acetylglucosamine transferase and ...
 207-332 7.72e-28

OTU (ovarian tumor) domain of bifunctional UDP-N-acetylglucosamine transferase and deubiquitinase ALG13 and similar proteins; Bifunctional UDP-N-acetylglucosamine transferase and deubiquitinase ALG13 is alco called asparagine-linked glycosylation 13 homolog, glycosyltransferase 28 domain-containing protein 1 (GLT28D1), or UDP-N-acetylglucosamine transferase subunit ALG13 homolog. It displays both glycosyltransferase (EC 2.4.1.141) and deubiquitinase (DUB)/ubiquitin thioesterase (EC 3.4.19.12) activities. With ALG14, it forms a UDP-N-acetylglucosamine transferase that catalyzes the second step of eukaryotic N-linked glycosylation in the endoplasmic reticulum. ALG13 variants cause a form of early infantile epileptic encephalopathy known as EIEE36 refractory seizures, neurodevelopmental impairment, and poor prognosis; given the essential role of ALG13 in glycosylation, it is also considered a congenital disorder of glycosylation (CDG). This subfamily also contains OTU domain-containing protein 4 (OTUD4), also called HIV-1-induced protein HIN-1, a DUB that specifically deubiquitinates 'Lys-63'-polyubiquitinated MYD88 adapter protein upon phosphorylation, triggering down-regulation of NF-kappa-B-dependent transcription of inflammatory mediators. This subfamily belongs to the OTU family of cysteine proteases that use a conserved cysteine, histidine, and an aspartate, as the catalytic triad.


Pssm-ID: 438590 [Multi-domain]  Cd Length: 130  Bit Score: 108.01  E-value: 7.72e-28
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 115430091 207 EKKGFVIKKMKEDGACLFRAVADQVYGDQDMHEVVRKHCMDYLMKNADYFSNYVTEDFTTYINRKRKNNCHGNHIEMQAM 286
Cdd:cd22753    6 DSLGLYRKHIPRDGSCLFRAVSEQLFFTQSYHQQVRQACVEYLEKNREEFEKFSEISFDDYLERLSDPKEWGGLLELEAL 85

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....*..
gi 115430091 287 AEMYNRPVEVYQ-SGTEPINTFHGIHQnndEPIRVSYHRNIHYNSVV 332
Cdd:cd22753   86 SLLYKVDFIVYSiPDQPPSNITNNGYP---KKIMLCYSGGNHYDSVY 129
OTU_OTUD3-like cd22756
OTU (ovarian tumor) domain of OTU domain-containing protein 3 and similar proteins; This ...
 213-331 7.03e-27

OTU (ovarian tumor) domain of OTU domain-containing protein 3 and similar proteins; This subfamily includes bilaterial OTU domain-containing protein 3 (OTUD3), Arabidopsis thaliana deubiquitinating enzyme OTU7, also called OTU domain-containing protein 7, and similar proteins. OTUD3 is a deubiquitinase (DUB)/ubiquitin thioesterase (EC 3.4.19.12) that hydrolyzes 'Lys-6'- and 'Lys-11'-linked polyubiquitin. It is an acetylation-dependent deubiquitinase that restricts innate antiviral immune signaling. It directly hydrolyzes lysine 63 (Lys63)-linked polyubiquitination of MAVS (mitochondrial antiviral-signaling protein) and shuts off innate antiviral immune response. OTUD3 can elicit tumor-suppressing or tumor-promoting activities in a cell- and tissue-dependent manner. It is a DUB for PTEN (phosphatase and tension homologue deleted on chromosome 10); the OTUD3-PTEN signaling axis plays a critical role in suppression of breast tumorigenesis. OTUD3 is also a DUB for glucose-regulated protein GRP78, stabilizing it and promoting lung tumorigenesis. OTU7 is a DUB that shows a preference for 'Lys-63' over 'Lys-48' over 'Met-1'-linked ubiquitin (UB) tetramers as substrates. This subfamily belongs to the OTU family of cysteine proteases that use a conserved cysteine, histidine, and an aspartate, as the catalytic triad.


Pssm-ID: 438593 [Multi-domain]  Cd Length: 131  Bit Score: 105.72  E-value: 7.03e-27
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 115430091 213 IKKMKEDGACLFRAVADQVYGDQDMHEVVRKHCMDYLMKNADYFSNYVT--------EDFTTYINRKRKNNCHGNHIEMQ 284
Cdd:cd22756    2 AKDITGDGNCLFRALSDQLYGDPDRHLEIRAEVVEYMRANPDDFKPFSEaatfaeddEAFEDYLARMAKDGTYGDNLEIV 81

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|.
gi 115430091 285 AMAEMYNRPVEVYQSGtePINTFHGIHQNNDEPIR----VSYHRNIHYNSV 331
Cdd:cd22756   82 AFARAYNVDVKVYQPD--PVYVISAPEDGSPGPARrvlhIAYHNWEHYSSV 130
OTU_232R-like cd22758
OTU (ovarian tumor) domain of Invertebrate iridescent virus putative ubiquitin thioesterase ...
 207-331 1.00e-25

OTU (ovarian tumor) domain of Invertebrate iridescent virus putative ubiquitin thioesterase 232R and similar proteins; This subfamily contains putative ubiquitin thioesterases 232R from Invertebrate iridescent virus and L96 from Tipula iridescent virus (TIV), Dictyostelium discoideum OTU domain-containing protein DDB_G0284757, and similar proteins. L96 may be involved in TIV genomic DNA packaging in a manner related to the Gag polyproteins of the mammalian viruses. Proteins in this subfamily contain an OTU domain. OTU domains typically function as deubiquitinases (DUBs)/ubiquitin thiolesterases (EC 3.4.19.12) that catalyze the thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin, a small regulatory protein that can be conjugated to a large range of target proteins. Protein ubiquitination is a post-translational modification of mostly Lys residues that regulates many cellular processes, including protein degradation, intracellular trafficking, cell signaling, autophagy, transcription, translation, and the DNA damage response. These DUBs may play important regulatory roles at the level of protein turnover by preventing degradation. They belong to the OTU family of cysteine proteases that use a conserved cysteine, histidine, and an aspartate, as the catalytic triad.


Pssm-ID: 438595 [Multi-domain]  Cd Length: 135  Bit Score: 102.35  E-value: 1.00e-25
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 115430091 207 EKKGFVIKKMKEDGACLFRAVADQVYGDQ--DMHEVVRKHCMDYLMKNADYFSNYV------TEDFTTYINRKRKNNCHG 278
Cdd:cd22758    2 KENGFEIRDVPGDGNCFFHAVSDQLYGNGieHSHKELRQQAVNYLRENPELYDGFFlsefdeEESWEEYLNRMSKDGTWG 81

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|....
gi 115430091 279 NHIEMQAMAEMYNRPVEVYQS-GTEPINTFHGIHQNNDEPIRVSYHRNIHYNSV 331
Cdd:cd22758   82 DHIILQAAANLFNVRIVIISSdGSDETTIIEPGNSKNGRTIYLGHIGENHYVSL 135
OTU_plant_OTU9-like cd22751
OTU (ovarian tumor) domain of plant deubiquitinating enzyme OTU9 and similar proteins; This ...
 210-331 6.36e-25

OTU (ovarian tumor) domain of plant deubiquitinating enzyme OTU9 and similar proteins; This subfamily contains Arabidopsis thaliana deubiquitinating enzymes OTU8, OTU9, OTU10, OTU11, and OTU12, and similar proteins from plants and other eukaryotes. OTU8-OTU12 are deubiquitinases (DUBs)/ubiquitin thiolesterases (EC 3.4.19.12) that catalyze the thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin, a small regulatory protein that can be conjugated to a large range of target proteins. Protein ubiquitination is a post-translational modification of mostly Lys residues that regulates many cellular processes, including protein degradation, intracellular trafficking, cell signaling, autophagy, transcription, translation, and the DNA damage response. These DUBs may play important regulatory roles at the level of protein turnover by preventing degradation. They belong to the OTU family of cysteine proteases that use a conserved cysteine, histidine, and an aspartate, as the catalytic triad.


Pssm-ID: 438588 [Multi-domain]  Cd Length: 134  Bit Score: 100.31  E-value: 6.36e-25
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 115430091 210 GFVIKKMKEDGACLFRAVADQVYGDQDMHEVVRKHCMDYLMKNAD-YFSNYVTEDFTTYINRKRKNNCHGNHIEMQAMAE 288
Cdd:cd22751    9 GLVERKVEGDGNCQFRALSDQLFGTQDHHAEVRELVVKQLRAHPElYYEFYVPEEYDEYLKKMSKDGEWGDELTLQAAAD 88

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....*..
gi 115430091 289 MYNRPVEVYQSgtEPINTFHGI-HQNNDEPIRV---SYHRNIHYNSV 331
Cdd:cd22751   89 AFGVKIHVITS--FEDNWFLEIePRGLVRSKRVlflSYWAEVHYNSI 133
OTU_OTUD6 cd22761
OTU (ovarian tumor) domain of OTU domain-containing proteins 6A and 6B and similar proteins; ...
 203-332 5.00e-23

OTU (ovarian tumor) domain of OTU domain-containing proteins 6A and 6B and similar proteins; This subfamily is composed of mammalian OTU domain-containing protein 6A (OTUD6A, also called DUBA-2) and vertebrate OTU domain-containing protein 6B (OTUD6B, also called DUBA-5), which are deubiquitinating enzymes/ubiquitinyl hydrolases (EC 3.4.19.12). OTUD6A hydrolyzes 'Lys-27'-, 'Lys-29'-, and 'Lys-33'-linked polyubiquitin chains, and may also be able to hydrolyze 'Lys-11'-linked ubiquitin chains. It deubiquitylates and stabilizes dynamin-related protein 1 (Drp1), a cytosolic protein responsible for mitochondrial fission and is essential in the initiation and development of several human diseases including cancer, thereby facilitating tumorigenesis. OTUD6B is a functional deubiquitinase in in vitro enzyme assays. It may play a role in the ubiquitin-dependent regulation of protein synthesis downstream of mTORC1, and may modify the ubiquitination of the protein synthesis initiation complex to repress translation. Biallelic variants in OTUD6B cause an intellectual disability syndrome that is associated with seizures and dysmorphic features. This subfamily belongs to the OTU family of cysteine proteases that use a conserved cysteine, histidine, and an aspartate, as the catalytic triad.


Pssm-ID: 438598 [Multi-domain]  Cd Length: 146  Bit Score: 95.26  E-value: 5.00e-23
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 115430091 203 KALQEKKGFVIKKMKEDGACLFRAVADQ---VYGDQDMHEVvRKHCMDYLMKNADYF----SN------YVTEDFTTYIN 269
Cdd:cd22761    2 KKILKERGLKIHEIPSDGDCLYNAIAHQlslRGIETSVEEL-RKQTADYMRENKDDFlpflTNpdtgdpLTEEEFEKYCD 80

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 115430091 270 RKRKNNCHGNHIEMQAMAEMYNRPVEVYQSGTEPIntFHGIHQNNDEPIRVSYHRNI-----HYNSVV 332
Cdd:cd22761   81 DVENTGAWGGQLELRALSHVLKRPIEVIQAEGPPI--IIGEEFKSGKPLILTYHRHAyglgeHYNSVE 146
OTU_OTUD4 cd22794
OTU (ovarian tumor) domain of OTU domain-containing protein 4 and similar proteins; OTU ...
 210-331 1.09e-21

OTU (ovarian tumor) domain of OTU domain-containing protein 4 and similar proteins; OTU domain-containing protein 4 (OTUD4), also called HIV-1-induced protein HIN-1, is a deubiquitinase that specifically deubiquitinates 'Lys-63'-polyubiquitinated MYD88 adapter protein upon phosphorylation, triggering down-regulation of NF-kappa-B-dependent transcription of inflammatory mediators. OTUD4 belongs to the OTU family of cysteine proteases that use a conserved cysteine, histidine, and an aspartate, as the catalytic triad.


Pssm-ID: 438615 [Multi-domain]  Cd Length: 130  Bit Score: 90.89  E-value: 1.09e-21
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 115430091 210 GFVIKKMKEDGACLFRAVADQVYGDQDMHEVVRKHCMDYLMKNADYFSNYVTEDFTTYINRKRKNNCHGNHIEMQAMAEM 289
Cdd:cd22794    9 GLYRKQIAKDGSCLFRAVAEQVFHTQSRHLEVRKACVDYLRRNREKFEAFIEGPFEQYLKNLENPKEWAGQVEISALSLM 88

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....
gi 115430091 290 YNRPVEVYQS-GTEPINtfhgIHQNN-DEPIRVSYHRNIHYNSV 331
Cdd:cd22794   89 YKRDFIIYQEpGKPPSN----VTENGfPDKILLCFSNGNHYDSV 128
OTU_fungi_OTU2-like cd22762
OTU (ovarian tumor) domain of fungal OTU domain-containing protein 2 and similar proteins; ...
 207-332 1.45e-18

OTU (ovarian tumor) domain of fungal OTU domain-containing protein 2 and similar proteins; This subfamily includes Schizosaccharomyces pombe and Saccharomyces cerevisiae OTU domain-containing protein 2 (OTU2) and similar proteins. S. pombe OTU2 is a ubiquitin thioesterase/hydrolase (EC 3.4.19.12) that can remove conjugated ubiquitin from protein substrates and may therefore play an important regulatory role at the level of protein turnover by preventing degradation. Fungal OTU2 bbelongs to the OTU family of cysteine proteases that use a conserved cysteine, histidine, and an aspartate, as the catalytic triad.


Pssm-ID: 438599 [Multi-domain]  Cd Length: 142  Bit Score: 82.28  E-value: 1.45e-18
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 115430091 207 EKKGFVIKKMKEDGACLFRAVADQ---VYGDQDM-HEVVRKHCMDYLMKNADYFSNYVTE------DFTTYINRKRKNNC 276
Cdd:cd22762    3 EELGLEEHDIKPDGHCLFAAIADQlqlRGSEINLdYKELRKLAAEYIRKHPDDFEPFLFEetdeleDIDEYCKKIENTAE 82

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|.
gi 115430091 277 HGNHIEMQAMAEMYNRPVEVYQSGTEPInTFHGIHQNNDEPIRVSYHRNI-----HYNSVV 332
Cdd:cd22762   83 WGGELELLALAKAFGVPIHVVQAEGRVI-KINEEGDSDKPELWLAYYKHSyglgeHYNSLR 142
OTU pfam02338
OTU-like cysteine protease; This family is comprised of a group of predicted cysteine ...
 219-328 1.58e-18

OTU-like cysteine protease; This family is comprised of a group of predicted cysteine proteases, homologous to the Ovarian Tumour (OTU) gene in Drosophila. Members include proteins from eukaryotes, viruses and pathogenic bacterium. The conserved cysteine and histidine, and possibly the aspartate, represent the catalytic residues in this putative group of proteases.


Pssm-ID: 426728 [Multi-domain]  Cd Length: 127  Bit Score: 81.73  E-value: 1.58e-18
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 115430091  219 DGACLFRAVADQVYGDQDM-----HEVVRKHCMDYLMKNADYFSNYVTEDFTTYINRKRKNNCHGNHIEMQAMAEMYNRP 293
Cdd:pfam02338   3 DGNCLYRSISHQLWGVHDVlrkmlVQELRETLAEYMREHKEEFEPFLEDDETGDIIEIEQTGAWGGEIEIFALAHILRRP 82

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|
gi 115430091  294 VEVYQSGTEPIN---TFHGIHQNN--DEPIRVSYHRNIHY 328
Cdd:pfam02338  83 IIVYKSEGGEELgglKEYGIYLPLgwDPSLCLVYPRHLYY 122
OTU_CeDUB-like cd22755
OTU (ovarian tumor) domain of Caenorhabditis elegans deubiquitylating enzyme with USP/UBP and ...
 213-332 3.56e-18

OTU (ovarian tumor) domain of Caenorhabditis elegans deubiquitylating enzyme with USP/UBP and OTU domains, and similar proteins; This subfamily is composed of mostly uncharacterized proteins containing an OTU domain, similar to Caenorhabditis elegans deubiquitylating enzyme with USP/UBP and OTU domains. OTU domain-containing proteins function as deubiquitinases (DUBs)/ubiquitin thiolesterases (EC 3.4.19.12) that catalyze the thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin, a small regulatory protein that can be conjugated to a large range of target proteins. Protein ubiquitination is a post-translational modification of mostly Lys residues that regulates many cellular processes, including protein degradation, intracellular trafficking, cell signaling, autophagy, transcription, translation, and the DNA damage response. These DUBs may play important regulatory roles at the level of protein turnover by preventing degradation. They belong to the OTU family of cysteine proteases that use a conserved cysteine, histidine, and an aspartate, as the catalytic triad.


Pssm-ID: 438592 [Multi-domain]  Cd Length: 132  Bit Score: 80.77  E-value: 3.56e-18
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 115430091 213 IKKMKEDGACLFRAVADQVYGDQDMHEVVRKHCMDYLMKNADYFSNYVTEDFTT---YINRKRKNNCH--GNHIEMQAMA 287
Cdd:cd22755    3 TIKIVGDGNCFFRALSYAITGSEKYHRKIRKAIVDFLEKNPDEFRNLLRSDYESveeYLEKSRMRYDGtwATDVEIFAAA 82

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|.
gi 115430091 288 EMYNRPVEVYQSGTE------PiNTFHGIHQNNDEPIRVSYHRNIHYNSVV 332
Cdd:cd22755   83 TLLGVDIYVYSKGGYkwllysP-RFKLGKRNGSREAIYLKNTNGNHFEPVV 132
OTU_P87_VP80-like cd22757
OTU (ovarian tumor) domain of nucleopolyhedrovirus P87/VP80 protein and similar proteins; The ...
 211-329 6.69e-18

OTU (ovarian tumor) domain of nucleopolyhedrovirus P87/VP80 protein and similar proteins; The VP80 protein is a capsid-associated structural protein that was first identified as P87 in Orgyia pseudotsugata multicapsid nuclear polyhedrosis virus (OpMNPV); its homologs are found only in NPV genomes. The Autographa californica multicapsid nucleopolyhedrovirus (AcMNPV) VP80 protein is essential for the formation of both budded virus (BV) and occlusion-derived virus (ODV). It has also been shown to interact with the virus-triggered, nuclear F-actin cytoskeleton. P87/VP80 contains an N-terminal OTU domain. OTU domains typically function as deubiquitinases (DUBs)/ubiquitin thiolesterases (EC 3.4.19.12) that catalyze the thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin, a small regulatory protein that can be conjugated to a large range of target proteins. Protein ubiquitination is a post-translational modification of mostly Lys residues that regulates many cellular processes, including protein degradation, intracellular trafficking, cell signaling, autophagy, transcription, translation, and the DNA damage response. These DUBs may play important regulatory roles at the level of protein turnover by preventing degradation. They belong to the OTU family of cysteine proteases that use a conserved cysteine, histidine, and an aspartate, as the catalytic triad.


Pssm-ID: 438594 [Multi-domain]  Cd Length: 128  Bit Score: 79.94  E-value: 6.69e-18
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 115430091 211 FVIKKMKEDGACLFRAVADQVYGDQDMHEVVRKHCMDYLMKNADYFSNYVTE-DFTTYINRKR------KNNCHGNHIEM 283
Cdd:cd22757    1 FRVIPIPGDGACLFRALSYLLYGTQSRHLEVRKEVVDYVVNNWDEFSIYTHDsEGNNYKSAEEyradmsKPGTYGTLCEL 80

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....*..
gi 115430091 284 QAMAEMYNRPVEVYQSGTEpINTFhGIHQNNDEPIRVSYH-RNIHYN 329
Cdd:cd22757   81 VAAAELYPFHFEVYRNGKL-YASF-GDPSNPVKRLKFSGDlSNGHFD 125
OTU_plant_OTU5-like cd22797
OTU (ovarian tumor) domain of deubiquitinating enzyme OTU5 from plants and similar proteins; ...
 210-332 2.75e-15

OTU (ovarian tumor) domain of deubiquitinating enzyme OTU5 from plants and similar proteins; Deubiquitinating enzyme OTU5, also called OTU domain-containing protein 6, is a deubiquitinase (DUB) or ubiquitin thiolesterase (EC 3.4.19.12) that catalyzes the thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin, a small regulatory protein that can be conjugated to a large range of target proteins. Protein ubiquitination is a post-translational modification of mostly Lys residues that regulates many cellular processes, including protein degradation, intracellular trafficking, cell signaling, autophagy, transcription, translation, and the DNA damage response. OTU5 is an inactive cysteine protease. It regulates gene expression by contributing to chromatin organization and DNA methylation patterns (e.g. H3K4me3 and H3K27me3). It is required for phosphate (Pi) homeostasis. OTU5 belongs to the OTU family of cysteine proteases that use a conserved cysteine, histidine, and an aspartate, as the catalytic triad.


Pssm-ID: 438618 [Multi-domain]  Cd Length: 149  Bit Score: 73.15  E-value: 2.75e-15
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 115430091 210 GFVIKKMKEDGACLFRAVADQVYGDQDMHEVV-----RKHCMDYLMKNADYFSNYV---------TEDFTTYINRKRKNN 275
Cdd:cd22797    9 GLAIKEIKADGHCLYRAVEDQLQLRGGGAPAPdyqqlRELAADYMRAHPDDFLPFLededeggdgDEAFEAYCREVESTA 88

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|..
gi 115430091 276 CHGNHIEMQAMAEMYNRPVEVYqSGTEPINTFHGIHQNNDEPIRVSYHRNI-----HYNSVV 332
Cdd:cd22797   89 AWGGQLELGALAHALRRHIKVY-SAGMPDVEMGEEYAGTGPPLRLCYHRHAfglgeHYNSVV 149
OTU_ALG13 cd22795
OTU (ovarian tumor) domain of bifunctional UDP-N-acetylglucosamine transferase and ...
 210-331 2.67e-14

OTU (ovarian tumor) domain of bifunctional UDP-N-acetylglucosamine transferase and deubiquitinase ALG13; Bifunctional UDP-N-acetylglucosamine transferase and deubiquitinase ALG13 is also called asparagine-linked glycosylation 13 homolog, glycosyltransferase 28 domain-containing protein 1 (GLT28D1), or UDP-N-acetylglucosamine transferase subunit ALG13 homolog. It displays both glycosyltransferase (EC 2.4.1.141) and deubiquitinase (DUB)/ubiquitin thioesterase (EC 3.4.19.12) activities. With ALG14, it forms a UDP-N-acetylglucosamine transferase that catalyzes the second step of eukaryotic N-linked glycosylation in the endoplasmic reticulum. ALG13 variants cause a form of early infantile epileptic encephalopathy known as EIEE36 refractory seizures, neurodevelopmental impairment, and poor prognosis; given the essential role of ALG13 in glycosylation, it is also considered a congenital disorder of glycosylation (CDG). ALG13 belongs to the OTU family of cysteine proteases that use a conserved cysteine, histidine, and an aspartate, as the catalytic triad.


Pssm-ID: 438616 [Multi-domain]  Cd Length: 130  Bit Score: 69.84  E-value: 2.67e-14
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 115430091 210 GFVIKKMKEDGACLFRAVADQVYGDQDMHEVVRKHCMDYLMKNADYFSNYVTEDFTTYINRKRKNNCHGNHIEMQAMAEM 289
Cdd:cd22795    9 GLYRKLTAKDASCLFRAVSEQLFLCQIHHLEIRKACVSYMRANQCNFESYVEGSFEKYLERLEDPKESAGQLEISALSLI 88

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|...
gi 115430091 290 YNRPVEVYQS-GTEPIntfHGIHQNNDEPIRVSYHRNIHYNSV 331
Cdd:cd22795   89 YNRDFILYRYpGKPPT---YATDNGFEDKILLCCSSNGHYDSV 128
OTU_OTUD1 cd22747
OTU (ovarian tumor) domain of OTU domain-containing protein 1 and similar proteins; OTU ...
 219-331 4.11e-12

OTU (ovarian tumor) domain of OTU domain-containing protein 1 and similar proteins; OTU domain-containing protein 1 (OTUD1), also called DUBA-7 in humans, is a deubiquitinating enzyme/ubiquitinyl hydrolase (EC 3.4.19.12) that specifically hydrolyzes 'Lys-63'-linked polyubiquitin to monoubiquitin; this specificity is facilitated by the C-terminal Ub-interacting motif (UIM) of OTUD1. It interacts and promotes the deubiquitination of myeloid cell leukemia 1 (MCL1), a pro-survival Bcl-2 family protein that plays important roles in cell survival, proliferation, differentiation and tumorigenesis. OTUD1 also deubiquitinates IFN regulatory factor 3 (IRF3) and attenuates its function; IRF3 is critical for the transcription of type I IFNs in defensing virus and promoting inflammatory responses. Loss-of-function mutations of OTUD1 associated with multiple autoimmune diseases including systemic lupus erythematosus (SLE), rheumatoid arthritis (RA), ulcerative colitis (UC) and Hashimoto's thyroiditis (HT). OTUD1 belongs to the OTU family of cysteine proteases that use a conserved cysteine, histidine, and an aspartate, as the catalytic triad.


Pssm-ID: 438584 [Multi-domain]  Cd Length: 149  Bit Score: 64.06  E-value: 4.11e-12
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 115430091 219 DGACLFRAVADQVYGDQDMHEVVRKHCMDYLMKNADYFSNYVTEDFTTYINRKRKNNCHGNHIEMQAMAEMYNRPVEVYQ 298
Cdd:cd22747   29 DGNCLYRAVSKAVYGDQALHRELREQTVHYIADHLDEFNPIIEGDVGEFLIKAAQDGAWAGYPELLAMGQMLNVNIRLTT 108

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|
gi 115430091 299 SGT--EPINTfHGIHQNNDE-----PIRVSYHRNIHYNSV 331
Cdd:cd22747  109 GGSleSPTVS-TMVHYLGPEdsgkpSIWLSWLSNGHYDAV 147
OTU_OTUD3 cd22770
OTU (ovarian tumor) domain of OTU domain-containing protein 3 and similar proteins; OTU ...
 201-331 1.99e-10

OTU (ovarian tumor) domain of OTU domain-containing protein 3 and similar proteins; OTU domain-containing protein 3 (OTUD3) is a deubiquitinase (DUB)/ubiquitin thioesterase (EC 3.4.19.12) that hydrolyzes 'Lys-6'- and 'Lys-11'-linked polyubiquitin. It is an acetylation-dependent deubiquitinase that restricts innate antiviral immune signaling. It directly hydrolyzes lysine 63 (Lys63)-linked polyubiquitination of MAVS (mitochondrial antiviral-signaling protein) and shuts off innate antiviral immune response. OTUD3 can elicit tumor-suppressing or tumor-promoting activities in a cell- and tissue-dependent manner. It is a DUB for PTEN (phosphatase and tension homologue deleted on chromosome 10); the OTUD3-PTEN signaling axis plays a critical role in suppression of breast tumorigenesis. OTUD3 is also a DUB for glucose-regulated protein GRP78, stabilizing it and promoting lung tumorigenesis. It belongs to the OTU family of cysteine proteases that use a conserved cysteine, histidine, and an aspartate, as the catalytic triad.


Pssm-ID: 438607 [Multi-domain]  Cd Length: 145  Bit Score: 59.22  E-value: 1.99e-10
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 115430091 201 FEKALQeKKGFVIKKMKEDGACLFRAVADQVYGDQDMHEVVRKHCMDYLMKNADYFSNYVTED--FTTYINRKRKNNCHG 278
Cdd:cd22770    5 FANQLQ-ALGLKLRDIPGDGNCLFRALGDQLEGHSRNHLKHRQETVQYMIEHREDFEPFVEDDvpFDKHVANLSKPGTYA 83

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|...
gi 115430091 279 NHIEMQAMAEMYNRPVEVYQSGTePINTFHGIHQNNDEPIRVSYHRNIHYNSV 331
Cdd:cd22770   84 GNDAIVAFARLHQVNVVIHQLNA-PLWQIRGTEKSSSRELHISYHNGDHYSSV 135
OTU_plant_OTU3_4-like cd22746
OTU (ovarian tumor) domain of deubiquitinating enzymes OTU3 and OTU4 from plants, and similar ...
 211-332 4.79e-09

OTU (ovarian tumor) domain of deubiquitinating enzymes OTU3 and OTU4 from plants, and similar proteins; Deubiquitinating enzyme OTU3 (also called OTU domain-containing protein 3) and deubiquitinating enzyme OTU4 (also called OTU domain-containing protein 4) are deubiquitinases (DUBs) or ubiquitin thiolesterases (EC 3.4.19.12) that catalyze the thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin, a small regulatory protein that can be conjugated to a large range of target proteins. Protein ubiquitination is a post-translational modification of mostly Lys residues that regulates many cellular processes, including protein degradation, intracellular trafficking, cell signaling, autophagy, transcription, translation, and the DNA damage response. OTU3 and OTU4 may play important regulatory roles at the level of protein turnover by preventing degradation. They belong to the OTU family of cysteine proteases that use a conserved cysteine, histidine, and an aspartate, as the catalytic triad.


Pssm-ID: 438583 [Multi-domain]  Cd Length: 141  Bit Score: 54.97  E-value: 4.79e-09
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 115430091 211 FVIKKMKEDGACLFRAVA----------------DQVYGDQ---DMHEVVRKHCMDYLMKNADyfsnyVTEDFTTYINRK 271
Cdd:cd22746    2 LRVVPVKGDGRCLFRAVArglalatggrplserrERADADAlrkAVVEEIRKRRDELFEGSLV-----IEGDFDAYCQRM 76

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 115430091 272 RKNNCHGNHIEMQAMAEMYNRPVEVY-----QSGTEPINTfHGIHQNNDEPIRVSYHRNIHYNSVV 332
Cdd:cd22746   77 SHPDTWGGEPELLMLADVLQRPIAVYlptpgKGGLRKIQE-YGEEYLGGEPIRLLYNGGNHYDLLL 141
OTU_plant_OTU4-like cd22760
OTU (ovarian tumor) domain of deubiquitinating enzyme OTU4 from plants and similar proteins; ...
 210-332 1.52e-07

OTU (ovarian tumor) domain of deubiquitinating enzyme OTU4 from plants and similar proteins; Deubiquitinating enzyme OTU4, also called OTU domain-containing protein 4, is a deubiquitinase (DUB) or ubiquitin thiolesterase (EC 3.4.19.12) that catalyzes the thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin, a small regulatory protein that can be conjugated to a large range of target proteins. Protein ubiquitination is a post-translational modification of mostly Lys residues that regulates many cellular processes, including protein degradation, intracellular trafficking, cell signaling, autophagy, transcription, translation, and the DNA damage response. OTU4 may play an important regulatory role at the level of protein turnover by preventing degradation. OTU4 belongs to the OTU family of cysteine proteases that use a conserved cysteine, histidine, and an aspartate, as the catalytic triad.


Pssm-ID: 438597 [Multi-domain]  Cd Length: 138  Bit Score: 50.45  E-value: 1.52e-07
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 115430091 210 GFVIKKMKEDGACLFRAVAdqvYG----------DQDMHEV----VRKHCMDYLMKNADYFSNYVTEDFTTYINRKRKNN 275
Cdd:cd22760    1 DYRVHGIAGDGRCLFRAVA---HGeclargkaapDEEREREladeLRTRAADELVKRREETEWFIEGDFDEYVARMRRPG 77

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|.
gi 115430091 276 CHGNHIEMQAMAEMYNRPVEVY--QSGTEPINTF--HGIHQNNDEPIRVSYHRNIHYNSVV 332
Cdd:cd22760   78 VWGGEPELLMLSHVLQRPITVYmaDEGEGGLISIaeYGQEYGKGNPIRVLFHGFGHYEALL 138
OTU_VRTN cd22791
OTU (ovarian tumor) domain of vertnin and similar proteins; Vertnin (VRTN) is an OTU ...
 219-327 8.67e-05

OTU (ovarian tumor) domain of vertnin and similar proteins; Vertnin (VRTN) is an OTU domain-containing protein that is required for the development of thoracic vertebrae in mammals. OTU domains typically function as deubiquitinases (DUBs)/ubiquitin thiolesterases (EC 3.4.19.12) that catalyze the thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin, a small regulatory protein that can be conjugated to a large range of target proteins. Protein ubiquitination is a post-translational modification of mostly Lys residues that regulates many cellular processes, including protein degradation, intracellular trafficking, cell signaling, autophagy, transcription, translation, and the DNA damage response. These DUBs may play important regulatory roles at the level of protein turnover by preventing degradation. Vertnin and some subfamily members do not possess the conserved catalytic residues and may not have DUB activity. VRTN gene is associated with variations in vertebral number.


Pssm-ID: 438612  Cd Length: 137  Bit Score: 42.59  E-value: 8.67e-05
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 115430091 219 DGACLFRAVADQVYGDQDMHEVVRKHCMDYLMKNADYFSnyvtEDFTTYINRKRKNNCHGNHIEMQAMAEMYNRPVE-VY 297
Cdd:cd22791    9 DGNCLFRAASLLLFGDESLHLELRLRTVLELVLNSEFYE----AIYEAEIKATCKPGSYSGIWHIYALSSVLQRPIFsVY 84

                         90       100       110
                 ....*....|....*....|....*....|
gi 115430091 298 QsgtepintfhgihQNNDEPIRVSYHRNIH 327
Cdd:cd22791   85 P-------------EVGNQKIRPLLNRTIR 101
OTU_OTU1 cd22745
OTU (ovarian tumor) domain of ubiquitin thioesterase OTU1 and similar proteins; Ubiquitin ...
 211-332 3.80e-04

OTU (ovarian tumor) domain of ubiquitin thioesterase OTU1 and similar proteins; Ubiquitin thioesterase (EC 3.4.19.12) OTU1 is also called OTU domain-containing protein 1 in yeast, while human OTU1 is also called HIV-1-induced protease 7 (HIN7), DUBA-8, or OTU domain-containing protein 2 (OTUD2). OTU1 is a deubiquitinase (DUB) that catalyzes the thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin, a small regulatory protein that can be conjugated to a large range of target proteins. Protein ubiquitination is a post-translational modification of mostly Lys residues that regulates many cellular processes, including protein degradation, intracellular trafficking, cell signaling, autophagy, transcription, translation, and the DNA damage response. OTU1 has been implicated in the ER-associated degradation (ERAD) pathway. In yeast, it counteracts the activity of Ufd2 by deubiquitinating Ufd2 substrates; Ufd2 is a E4 ubiquitin ligase that interacts with Cdc48, an AAA ATPase that plays a central role in the ERAD pathway by chaperoning proteins to the proteasome for destruction. OTU1 also functions as a substrate-processing factor of valosin-containing protein (VCP, the mammalian counterpart of yeast Cdc48) that is required for the retrotranslocation of the ERAD pathway. OTU1 has been shown to preferentially hydrolyze polyubiquitin chains with Lys48 linkages. It contains ubiquitin-like (Ubl) domain with a beta-grasp Ubl fold, a C2H2-type zinc finger, and an OTU (ovarian tumor) domain. This model represents the OTU domain that interacts with ubiquitin and possesses catalytic activity. OTU1 belongs to the OTU family of cysteine proteases that use a conserved cysteine, histidine, and an aspartate, as the catalytic triad. This family also contains plant OTU1 and OTU2.


Pssm-ID: 438582  Cd Length: 161  Bit Score: 41.32  E-value: 3.80e-04
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 115430091 211 FVIKKMKEDGACLFRAVADQVYGDQDMH-EVVRKHCMDYLMKNADYFSNYV----TEDfttYINRKRKNNCHGNHIEMQA 285
Cdd:cd22745    3 LVRRVVPDDNSCLFTSISYLLEGGLLDSaPELREIVADAILSDPDTYNEAIlgkpPDE---YCAWILKPDSWGGAIELSI 79

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|.
gi 115430091 286 MAEMYNrpVEVY----QSGTepINTFhGIHQNNDEPIRVSYHrNIHYNSVV 332
Cdd:cd22745   80 LSKHFG--VEICvvdvQTGR--VDRF-GEDKGYSKRIFLLYS-GIHYDALA 124
OTU_RNAP_L_virus cd21880
OTU (ovarian tumor) domain of viral RNA-directed RNA polymerase L; RNA-directed RNA polymerase ...
 211-294 5.16e-04

OTU (ovarian tumor) domain of viral RNA-directed RNA polymerase L; RNA-directed RNA polymerase L is also called protein L, large structural protein, replicase, transcriptase, or ubiquitin thioesterase. It displays RNA-directed RNA polymerase (EC 2.7.7.48), deubiquitinase (DUB)/ubiquitin thiolesterase (EC 3.4.19.12), and deISGylating activities. It is a viral homolog of ovarian tumor protease (vOTU) that has been implicated in the downregulation of type I interferon immune response by removing post-translational modifying proteins ubiquitin (Ub) and the Ub-like interferon-simulated gene 15 (ISG15) from host cellular proteins. The attachment of Ub and ISG15 to cellular proteins mediates important innate antiviral responses, and their removal inhibits these antiviral pathways. This subfamily belongs to the OTU family of cysteine proteases that use a conserved cysteine, histidine, and an aspartate, as the catalytic triad.


Pssm-ID: 438580  Cd Length: 148  Bit Score: 40.66  E-value: 5.16e-04
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 115430091 211 FVIKKMKEDGACLFRAVADQVYGDQDMHEVVRKHCMDYLMKNADYFS----NYVTEDftTYINRKRKNNCHGNHIEMQAM 286
Cdd:cd21880   22 FEIERVPGDGNCFFRSIAELLFDTEDEWRLVKNTIESYARANWDECPearlYYLSLE--EYLRDAMKDGYWGGSLEAEIL 99


                 ....*...
gi 115430091 287 AEMYNRPV 294
Cdd:cd21880  100 SKALGITI 107
DUF6264 pfam19779
Family of unknown function (DUF6264); This family of putative integral membrane proteins is ...
 433-538 1.92e-03

Family of unknown function (DUF6264); This family of putative integral membrane proteins is functionally uncharacterized. This family of proteins is found in bacteria. Proteins in this family are typically between 179 and 218 amino acids in length.


Pssm-ID: 466182  Cd Length: 182  Bit Score: 39.56  E-value: 1.92e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 115430091  433 EEWNARSPRQRSSAPSPEIPDPAHSDTAAKPPSPAGALALSKPPSPCAPGPSNQACVGP--DRP------------TSSS 498
Cdd:pfam19779  10 PGWQRAPIGDPAAAAAAAPPAAPAPAAPAPPAAPAAPPAAPPPPGAPAPGAPAAARRARrwDRIatiallvfglfnVLSS 89

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|
gi 115430091  499 LVSLypaLGYRAIMQEMSpTAFGLTDWEDDEILASVLAVS 538
Cdd:pfam19779  90 IPSL---LDLPSTLQTLF-AQLGIDEFTPPAAARTWGIVA 125
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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