ste24c prenyl protease type I, isoform D [Drosophila melanogaster]
Protein Classification
M48 family metallopeptidase( domain architecture ID 11574938)
M48 family metallopeptidase similar to CAAX prenyl protease 1 which proteolytically removes the C-terminal three residues of farnesylated A-factor mating pheromone
List of domain hits
| Name | Accession | Description | Interval | E-value | |||||||
| M48A_Zmpste24p_like | cd07343 | Peptidase M48 subfamily A, a type 1 CaaX endopeptidase; This family contains peptidase family ... |
28-445 | 9.13e-113 | |||||||
Peptidase M48 subfamily A, a type 1 CaaX endopeptidase; This family contains peptidase family M48 subfamily A which includes a number of well-characterized genes such as those found in humans (ZMPSTE24, also known as farnesylated protein-converting enzyme 1 or FACE-1 or Hs Ste24), Taenia solium metacestode (TsSte24p), Arabidopsis (AtSte24) and yeast (Ste24p). Ste24p contains the zinc metalloprotease motif (HEXXH), likely exposed on the cytoplasmic side. It is thought to be intimately associated with the endoplasmic reticulum (ER), regardless of whether its genes possess the conventional signal motif (KKXX) in the C-terminal. Proteins in this family proteolytically remove the C-terminal three residues of farnesylated proteins. Ste24p is involved in the post-translational processing of prelamin A to mature lamin A, a major component of the nuclear envelope. ZmpSte24 deficiency causes an accumulation of prelamin A leading to lipodystrophy and other disease phenotypes, while mutations in this gene or in that encoding its substrate, prelamin A, result in a series of human inherited diseases known as laminopathies, the most severe of which are Hutchinson Gilford progeria syndrome (HGPS) and restrictive dermopathy (RD) which arise due to unsuccessful maturation of prelamin A. Two forms of mandibuloacral dysplasia, a condition that causes a variety of abnormalities involving bone development, skin pigmentation, and fat distribution, are caused by mutations in two different genes; mutations in the LMNA gene, which normally provides instructions for making lamin A and lamin C, cause mandibuloacral dysplasia with A-type lipodystrophy (MAD-A), and mutations in the ZMPSTE24 gene cause mandibuloacral dysplasia with B-type lipodystrophy (MAD-B). Within cells, these genes are involved in maintaining the structure of the nucleus and may play a role in many cellular processes. Certain HIV protease inhibitors have been shown to inhibit the enzymatic activity of ZMPSTE24, but not enzymes involved in prelamin A processing. : Pssm-ID: 320702 [Multi-domain] Cd Length: 405 Bit Score: 337.53 E-value: 9.13e-113
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| Name | Accession | Description | Interval | E-value | |||||||
| M48A_Zmpste24p_like | cd07343 | Peptidase M48 subfamily A, a type 1 CaaX endopeptidase; This family contains peptidase family ... |
28-445 | 9.13e-113 | |||||||
Peptidase M48 subfamily A, a type 1 CaaX endopeptidase; This family contains peptidase family M48 subfamily A which includes a number of well-characterized genes such as those found in humans (ZMPSTE24, also known as farnesylated protein-converting enzyme 1 or FACE-1 or Hs Ste24), Taenia solium metacestode (TsSte24p), Arabidopsis (AtSte24) and yeast (Ste24p). Ste24p contains the zinc metalloprotease motif (HEXXH), likely exposed on the cytoplasmic side. It is thought to be intimately associated with the endoplasmic reticulum (ER), regardless of whether its genes possess the conventional signal motif (KKXX) in the C-terminal. Proteins in this family proteolytically remove the C-terminal three residues of farnesylated proteins. Ste24p is involved in the post-translational processing of prelamin A to mature lamin A, a major component of the nuclear envelope. ZmpSte24 deficiency causes an accumulation of prelamin A leading to lipodystrophy and other disease phenotypes, while mutations in this gene or in that encoding its substrate, prelamin A, result in a series of human inherited diseases known as laminopathies, the most severe of which are Hutchinson Gilford progeria syndrome (HGPS) and restrictive dermopathy (RD) which arise due to unsuccessful maturation of prelamin A. Two forms of mandibuloacral dysplasia, a condition that causes a variety of abnormalities involving bone development, skin pigmentation, and fat distribution, are caused by mutations in two different genes; mutations in the LMNA gene, which normally provides instructions for making lamin A and lamin C, cause mandibuloacral dysplasia with A-type lipodystrophy (MAD-A), and mutations in the ZMPSTE24 gene cause mandibuloacral dysplasia with B-type lipodystrophy (MAD-B). Within cells, these genes are involved in maintaining the structure of the nucleus and may play a role in many cellular processes. Certain HIV protease inhibitors have been shown to inhibit the enzymatic activity of ZMPSTE24, but not enzymes involved in prelamin A processing. Pssm-ID: 320702 [Multi-domain] Cd Length: 405 Bit Score: 337.53 E-value: 9.13e-113
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| Peptidase_M48_N | pfam16491 | CAAX prenyl protease N-terminal, five membrane helices; The five N-terminal five transmembrane ... |
37-215 | 1.22e-43 | |||||||
CAAX prenyl protease N-terminal, five membrane helices; The five N-terminal five transmembrane alpha-helices of peptidase_M48 family proteins including the CAAX prenyl proteases reside completely within the membrane of the endoplasmic reticulum. Pssm-ID: 465138 Cd Length: 179 Bit Score: 151.10 E-value: 1.22e-43
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| HtpX | COG0501 | Zn-dependent protease with chaperone function [Posttranslational modification, protein ... |
228-442 | 7.22e-27 | |||||||
Zn-dependent protease with chaperone function [Posttranslational modification, protein turnover, chaperones]; Pssm-ID: 440267 [Multi-domain] Cd Length: 210 Bit Score: 107.28 E-value: 7.22e-27
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| PRK03982 | PRK03982 | heat shock protein HtpX; Provisional |
225-340 | 2.06e-03 | |||||||
heat shock protein HtpX; Provisional Pssm-ID: 235186 [Multi-domain] Cd Length: 288 Bit Score: 39.99 E-value: 2.06e-03
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| Name | Accession | Description | Interval | E-value | |||||||
| M48A_Zmpste24p_like | cd07343 | Peptidase M48 subfamily A, a type 1 CaaX endopeptidase; This family contains peptidase family ... |
28-445 | 9.13e-113 | |||||||
Peptidase M48 subfamily A, a type 1 CaaX endopeptidase; This family contains peptidase family M48 subfamily A which includes a number of well-characterized genes such as those found in humans (ZMPSTE24, also known as farnesylated protein-converting enzyme 1 or FACE-1 or Hs Ste24), Taenia solium metacestode (TsSte24p), Arabidopsis (AtSte24) and yeast (Ste24p). Ste24p contains the zinc metalloprotease motif (HEXXH), likely exposed on the cytoplasmic side. It is thought to be intimately associated with the endoplasmic reticulum (ER), regardless of whether its genes possess the conventional signal motif (KKXX) in the C-terminal. Proteins in this family proteolytically remove the C-terminal three residues of farnesylated proteins. Ste24p is involved in the post-translational processing of prelamin A to mature lamin A, a major component of the nuclear envelope. ZmpSte24 deficiency causes an accumulation of prelamin A leading to lipodystrophy and other disease phenotypes, while mutations in this gene or in that encoding its substrate, prelamin A, result in a series of human inherited diseases known as laminopathies, the most severe of which are Hutchinson Gilford progeria syndrome (HGPS) and restrictive dermopathy (RD) which arise due to unsuccessful maturation of prelamin A. Two forms of mandibuloacral dysplasia, a condition that causes a variety of abnormalities involving bone development, skin pigmentation, and fat distribution, are caused by mutations in two different genes; mutations in the LMNA gene, which normally provides instructions for making lamin A and lamin C, cause mandibuloacral dysplasia with A-type lipodystrophy (MAD-A), and mutations in the ZMPSTE24 gene cause mandibuloacral dysplasia with B-type lipodystrophy (MAD-B). Within cells, these genes are involved in maintaining the structure of the nucleus and may play a role in many cellular processes. Certain HIV protease inhibitors have been shown to inhibit the enzymatic activity of ZMPSTE24, but not enzymes involved in prelamin A processing. Pssm-ID: 320702 [Multi-domain] Cd Length: 405 Bit Score: 337.53 E-value: 9.13e-113
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| Peptidase_M48_N | pfam16491 | CAAX prenyl protease N-terminal, five membrane helices; The five N-terminal five transmembrane ... |
37-215 | 1.22e-43 | |||||||
CAAX prenyl protease N-terminal, five membrane helices; The five N-terminal five transmembrane alpha-helices of peptidase_M48 family proteins including the CAAX prenyl proteases reside completely within the membrane of the endoplasmic reticulum. Pssm-ID: 465138 Cd Length: 179 Bit Score: 151.10 E-value: 1.22e-43
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| HtpX | COG0501 | Zn-dependent protease with chaperone function [Posttranslational modification, protein ... |
228-442 | 7.22e-27 | |||||||
Zn-dependent protease with chaperone function [Posttranslational modification, protein turnover, chaperones]; Pssm-ID: 440267 [Multi-domain] Cd Length: 210 Bit Score: 107.28 E-value: 7.22e-27
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| Peptidase_M48 | pfam01435 | Peptidase family M48; Peptidase_M48 is the largely extracellular catalytic region of CAAX ... |
229-441 | 1.23e-26 | |||||||
Peptidase family M48; Peptidase_M48 is the largely extracellular catalytic region of CAAX prenyl protease homologs such as Human FACE-1 protease. These are metallopeptidases, with the characteriztic HExxH motif giving the two histidine-zinc-ligands and an adjacent glutamate on the next helix being the third. The whole molecule folds to form a deep groove/cleft into which the substrate can fit. Pssm-ID: 426263 [Multi-domain] Cd Length: 201 Bit Score: 106.36 E-value: 1.23e-26
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| M48A_Ste24p | cd07330 | Peptidase M48 CaaX prenyl protease type 1, an integral membrane, Zn-dependent protein; This ... |
117-445 | 1.20e-22 | |||||||
Peptidase M48 CaaX prenyl protease type 1, an integral membrane, Zn-dependent protein; This family of M48 CaaX prenyl protease 1-like family includes a number of well characterized genes such as those found in Taenia solium metacestode (TsSte24p), Arabidopsis (AtSte24), yeast Ste24p and human (Hs Ste24p) as well as several uncharacterized genes such as YhfN, some of which also containing tetratricopeptide (TPR) repeats. All members of this family contain the zinc metalloprotease motif (HEXXH), likely exposed on the cytoplasmic side. They are thought to be intimately associated with the endoplasmic reticulum (ER), regardless of whether their genes possess the conventional signal motif (KKXX) in the C-terminal. Proteins in this family proteolytically remove the C-terminal three residues of farnesylated proteins. The gene ZmpSte24, also known as FACE-1 in humans, a member of this family, is involved in the post-translational processing of prelamin A to mature lamin A, a major component of the nuclear envelope. ZmpSte24 deficiency causes an accumulation of prelamin A leading to lipodystrophy and other disease phenotypes while mutations in the protein lead to diseases of lamin processing (laminopathies), such as premature aging disease progeria and metabolic disorders. Some of these mutations map to the peptide-binding site. Pssm-ID: 320689 [Multi-domain] Cd Length: 285 Bit Score: 97.51 E-value: 1.20e-22
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| M48B_HtpX_like | cd07337 | Peptidase M48 subfamily B HtpX-like membrane-bound metallopeptidase; This HtpX family of ... |
175-441 | 1.36e-12 | |||||||
Peptidase M48 subfamily B HtpX-like membrane-bound metallopeptidase; This HtpX family of peptidase M48 subfamily B includes uncharacterized HtpX homologs and consists of proteins smaller than Ste24p, with homology restricted to the C-terminal half of Ste24p. HtpX expression is controlled by the Cpx stress response system, which senses abnormal membrane proteins. HtpX participates in the proteolytic quality control of these misfolded proteins by undergoing self-degradation and collaborating with FtsH, a membrane-bound and ATP-dependent protease, to eliminate them. HtpX, a zinc metalloprotease with an active site motif HEXXH, has an FtsH-like topology, and is capable of introducing endoproteolytic cleavages into SecY (also an FtsH substrate). However, HtpX does not have an ATPase activity and will only act against cytoplasmic regions of a target membrane protein. Thus, HtpX and FtsH have overlapping and/or complementary functions, which are especially important at high temperature; in E. coli and Xylella fastidiosa, HtpX is heat-inducible, while in Streptococcus gordonii it is not. Pssm-ID: 320696 [Multi-domain] Cd Length: 203 Bit Score: 66.57 E-value: 1.36e-12
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| M48B_HtpX_like | cd07338 | Peptidase M48 subfamily B HtpX-like membrane-bound metallopeptidase; This HtpX family of ... |
224-441 | 4.31e-07 | |||||||
Peptidase M48 subfamily B HtpX-like membrane-bound metallopeptidase; This HtpX family of peptidase M48 subfamily B includes uncharacterized HtpX homologs and consists of proteins smaller than Ste24p, with homology restricted to the C-terminal half of Ste24p. HtpX expression is controlled by the Cpx stress response system, which senses abnormal membrane proteins. HtpX participates in the proteolytic quality control of these misfolded proteins by undergoing self-degradation and collaborating with FtsH, a membrane-bound and ATP-dependent protease, to eliminate them. HtpX, a zinc metalloprotease with an active site motif HEXXH, has an FtsH-like topology, and is capable of introducing endoproteolytic cleavages into SecY (also an FtsH substrate). However, HtpX does not have an ATPase activity and will only act against cytoplasmic regions of a target membrane protein. Thus, HtpX and FtsH have overlapping and/or complementary functions, which are especially important at high temperature; in E. coli and Xylella fastidiosa, HtpX is heat-inducible, while in Streptococcus gordonii it is not. Pssm-ID: 320697 [Multi-domain] Cd Length: 216 Bit Score: 50.66 E-value: 4.31e-07
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| M48A_Ste24p-like | cd07345 | Peptidase M48 subfamily A-like, putative CaaX prenyl protease; This family contains peptidase ... |
65-447 | 5.04e-07 | |||||||
Peptidase M48 subfamily A-like, putative CaaX prenyl protease; This family contains peptidase family M48 subfamily A-like CaaX prenyl protease 1, most of which are uncharacterized. Some of these contain tetratricopeptide (TPR) repeats at the C-terminus. Proteins in this family contain the zinc metalloprotease motif (HEXXH), likely exposed on the cytoplasmic side. They are thought to be possibly associated with the endoplasmic reticulum (ER), regardless of whether their genes possess the conventional signal motif (KKXX) in the C-terminal. These proteins putatively remove the C-terminal three residues of farnesylated proteins proteolytically. Pssm-ID: 320704 [Multi-domain] Cd Length: 346 Bit Score: 51.51 E-value: 5.04e-07
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| Peptidase_M48_M56 | cd05843 | Peptidases M48 (Ste24 endopeptidase or htpX homolog) and M56 (in MecR1 and BlaR1), integral ... |
232-321 | 1.37e-05 | |||||||
Peptidases M48 (Ste24 endopeptidase or htpX homolog) and M56 (in MecR1 and BlaR1), integral membrane metallopeptidases; This family contains peptidase M48 (also known as Ste24 peptidase, Ste24p, Ste24 endopeptidase, a-factor converting enzyme, AFC1), M56 (also known as BlaR1 peptidase) as well as a novel family called minigluzincins. Peptidase M48 belongs to Ste24 endopeptidase family. Members of this family include Ste24 protease (peptidase M48A), protease htpX homolog (peptidase M48B), or CAAX prenyl protease 1, and mitochondrial metalloendopeptidase OMA1 (peptidase M48C). They proteolytically remove the C-terminal three residues of farnesylated proteins. They are integral membrane proteins associated with the endoplasmic reticulum and golgi, binding one zinc ion per subunit. In eukaryotes, Ste24p is required for the first NH2-terminal proteolytic processing event within the a-factor precursor, which takes place after COOH-terminal CAAX modification (C is cysteine; A is usually aliphatic; X is one of several amino acids) is complete. The Ste24p contains multiple membrane spans, a zinc metalloprotease motif (HEXXH), and a COOH-terminal ER retrieval signal (KKXX). Mutation studies have shown that the HEXXH protease motif, which is extracellular but adjacent to a transmembrane domain and therefore close to the membrane surface, is critical for Ste24p activity. Ste24p has limited homology to HtpX family of prokaryotic proteins; HtpX proteins, also part of the M48 peptidase family, are smaller and homology is restricted to the C-terminal half of Ste24p. HtpX expression is controlled by the Cpx stress response system, which senses abnormal membrane proteins; HtpX then undergoes self-degradation and collaborates with FtsH to eliminate these misfolded proteins. Peptidase M56 includes zinc metalloprotease domain in MecR1 and BlaR1. MecR1 is a transmembrane beta-lactam sensor/signal transducer protein that regulates the expression of an altered penicillin-binding protein PBP2a, which resists inactivation by beta-lactam antibiotics, in methicillin-resistant Staphylococcus aureus (MRSA). BlaR1 regulates the inducible expression of a class A beta-lactamase that hydrolytically destroys certain beta-lactam antibiotics in MRSA. Also included are a novel family of related proteins that consist of the soluble minimal scaffold similar to the catalytic domains of the integral-membrane metallopeptidase M48 and M56, thus called minigluzincins. Pssm-ID: 320682 [Multi-domain] Cd Length: 94 Bit Score: 43.59 E-value: 1.37e-05
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| PRK03982 | PRK03982 | heat shock protein HtpX; Provisional |
225-340 | 2.06e-03 | |||||||
heat shock protein HtpX; Provisional Pssm-ID: 235186 [Multi-domain] Cd Length: 288 Bit Score: 39.99 E-value: 2.06e-03
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| PRK02391 | PRK02391 | heat shock protein HtpX; Provisional |
170-446 | 2.72e-03 | |||||||
heat shock protein HtpX; Provisional Pssm-ID: 179418 [Multi-domain] Cd Length: 296 Bit Score: 39.53 E-value: 2.72e-03
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| M48B_HtpX_like | cd07339 | Peptidase M48 subfamily B HtpX-like membrane-bound metallopeptidase; This HtpX family of ... |
295-439 | 3.55e-03 | |||||||
Peptidase M48 subfamily B HtpX-like membrane-bound metallopeptidase; This HtpX family of peptidase M48 subfamily B includes uncharacterized HtpX homologs and consists of proteins smaller than Ste24p, with homology restricted to the C-terminal half of Ste24p. HtpX expression is controlled by the Cpx stress response system, which senses abnormal membrane proteins. HtpX participates in the proteolytic quality control of these misfolded proteins by undergoing self-degradation and collaborating with FtsH, a membrane-bound and ATP-dependent protease, to eliminate them. HtpX, a zinc metalloprotease with an active site motif HEXXH, has an FtsH-like topology, and is capable of introducing endoproteolytic cleavages into SecY (also an FtsH substrate). However, HtpX does not have an ATPase activity and will only act against cytoplasmic regions of a target membrane protein. Thus, HtpX and FtsH have overlapping and/or complementary functions, which are especially important at high temperature; in E. coli and Xylella fastidiosa, HtpX is heat-inducible, while in Streptococcus gordonii it is not. Pssm-ID: 320698 [Multi-domain] Cd Length: 229 Bit Score: 38.70 E-value: 3.55e-03
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| Blast search parameters | ||||
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