dsRNA-specific ribonuclease [Transcription];

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                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 72255535 197 AVIGALLQSSGPERAALFIRDFLITQMtgKELFEMWTIVNPMGLLVEELKKRNISAPESRLTRQSGSTTAlPLYFVGLYC 276
Cdd:COG0571 121 ALIGAIYLDGGLEAARKFVLRLFEPRL--EEIAPGGAGKDYKTALQEWLQARGLPLPEYEVVEEEGPDHA-KTFTVEVLV 197

                        90       100
                ....*....|....*....|....*...
gi 72255535 277 DKRLIAEGPGETVLVAEEEAARVALRKL 304
Cdd:COG0571 198 GGKVLGEGTGRSKKEAEQAAAKAALEKL 225

double-stranded RNA binding motif of mL44 subfamily proteins; The mitochondrion-specific ribosomal protein mL44 subfamily is composed of mitochondrial 54S ribosomal protein L3 (MRPL3) and mitochondrial 39S ribosomal protein L44 (MRPL44). MRPL3 (also known as mitochondrial large ribosomal subunit protein mL44) is a component of the mitochondrial ribosome (mitoribosome), a dedicated translation machinery responsible for the synthesis of mitochondrial genome-encoded proteins, including at least some of the essential transmembrane subunits of the mitochondrial respiratory chain. MRPL44 (also called L44mt, MRP-L44, or mitochondrial large ribosomal subunit protein mL44) is a component of the 39S subunit of mitochondrial ribosome. It may play a role in the assembly/stability of nascent mitochondrial polypeptides exiting the ribosome. Members of this family contain a RNase III-like domain and a double-stranded RNA binding motif (DSRM). DSRM is not sequence specific, but highly specific for dsRNAs of various origin and structure.

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                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 72255535 236 NPMGLLVEELKKRNISAPESRLTRQSGSTTALPLYFVGLYCDKRLIAEGPGETVLVAEEEAARVALRKLY 305
Cdd:cd20315   2 NPTRELAEILRREGIEKPVSRLIAESGRLTKSPVYIVGVYCDEETIGEGYGYSIKEAKAEAATDALMKLF 71

double-stranded RNA binding motif of ribonuclease III (RNase III) and similar proteins; RNase III (EC 3.1.26.3; also known as ribonuclease 3) digests double-stranded RNA formed within single-strand substrates, but not RNA-DNA hybrids. It is involved in the processing of rRNA precursors, viral transcripts, some mRNAs, and at least 1 tRNA (metY, a minor form of tRNA-init-Met). It cleaves the 30S primary rRNA transcript to yield the immediate precursors to the 16S and 23S rRNAs. The cleavage can occur in assembled 30S, 50S, and even 70S subunits and is influenced by the presence of ribosomal proteins. The RNase III family also includes the mitochondrion-specific ribosomal protein mL44 subfamily, which is composed of mitochondrial 54S ribosomal protein L3 (MRPL3) and mitochondrial 39S ribosomal protein L44 (MRPL44). Members of this family contain an RNase III domain and a C-terminal double-stranded RNA binding motif (DSRM). DSRM is not sequence specific, but highly specific for dsRNAs of various origin and structure.

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                ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 72255535 236 NPMGLLVEELKKRNISAPESRLTRQSGsTTALPLYFVGLYCDKRLIAEGPGETVLVAEEEAARVALRKL 304
Cdd:cd10845   2 DYKTALQEYLQKRGLPLPEYELVEEEG-PDHNKTFTVEVKVNGKVIGEGTGRSKKEAEQAAAKAALEKL 69

double-stranded RNA binding motif of metazoan ribonuclease III (RNase III) and similar proteins; RNase III (EC 3.1.26.3; also known as Drosha, or ribonuclease 3) is a double-stranded RNA (dsRNA)-specific endoribonuclease that is involved in the initial step of microRNA (miRNA) biogenesis. It is a component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, RNase III cleaves the 3' and 5' strands of a stem-loop in pri-miRNAs (processing center 11 bp from the dsRNA-ssRNA junction) to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs. It is also involved in pre-rRNA processing. Metazoan RNase III is a larger protein than bacterial RNase III. It contains two RNase III domains in the C-terminal half of the protein followed by a double-stranded RNA binding motif (DSRM). DSRM is not sequence specific, but highly specific for dsRNAs of various origin and structure.

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                ....*....|....*....|....*....|....*....|....*....|....*....|..
gi 72255535 243 EELKKRNIsaPESRLTRQSGSTTAlPLYFVGLYCDKRLIAEGPGETVLVAEEEAARVALRKL 304
Cdd:cd19877  17 EGKKEPDI--PEYKVLQKSGPTNT-RVYTVAVYFRGERIATGTGSSIQQAEMNAAEKALEKL 75