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Conserved domains on  [gi|62955755|ref|NP_001017891|]
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uncharacterized protein LOC550590 [Danio rerio]

Protein Classification

transposase family protein( domain architecture ID 10659611)

transposase family protein might bind to the end of a transposon and catalyze the movement of the transposon to another part of the genome by a cut and paste mechanism or a replicative transposition mechanism

Graphical summary

 Zoom to residue level

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List of domain hits

 Name Accession Description Interval E-value
DDE_Tnp_4 pfam13359
DDE superfamily endonuclease; This family of proteins are related to pfam00665 and are ...
 339-498 2.94e-38

DDE superfamily endonuclease; This family of proteins are related to pfam00665 and are probably endonucleases of the DDE superfamily. Transposase proteins are necessary for efficient DNA transposition. This domain is a member of the DDE superfamily, which contain three carboxylate residues that are believed to be responsible for coordinating metal ions needed for catalysis. The catalytic activity of this enzyme involves DNA cleavage at a specific site followed by a strand transfer reaction.


:

Pssm-ID: 463855 [Multi-domain]  Cd Length: 158  Bit Score: 137.02  E-value: 2.94e-38
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 62955755   339 VDSIEIDIDRVSNGKPKAQMVPQNKK--TLKYLICSIPRGAITFISKPRESQVDDKSLIESSGLLKKIQ----SGDLVLS 412
Cdd:pfam13359   1 VDGTEIPIQRPSNLEARGQTYSNYKHhnTLKVLIVVTPNGKIIFVSKGYPGSVSDKSILENSGLLKALNlafgPGDYILA 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 62955755   413 ECGFDIRDGVGLTCAEVDEGQLKVRDVETTRQISNLRLHVKRVTACVRTKYTFLNGPVLVSMtvpdvgedITFLDKIVTV 492
Cdd:pfam13359  81 DKGFPISDWLLVPYKKPGGAQLSPEEVEFNRRIASARIHVERVIGRLKGRFRILRGRLPISL--------PERLDKIVTV 152


                  ....*.
gi 62955755   493 CCALTN 498
Cdd:pfam13359 153 CCVLHN 158
THAP smart00980
The THAP domain is a putative DNA-binding domain (DBD) and probably also binds a zinc ion; It ...
 4-95 4.93e-16

The THAP domain is a putative DNA-binding domain (DBD) and probably also binds a zinc ion; It features the conserved C2CH architecture (consensus sequence: Cys - 2-4 residues - Cys - 35-50 residues - Cys - 2 residues - His). Other universal features include the location of the domain at the N-termini of proteins, its size of about 90 residues, a C-terminal AVPTIF box and several other conserved residues. Orthologues of the human THAP domain have been identified in other vertebrates and probably worms and flies, but not in other eukaryotes or any prokaryotes.


:

Pssm-ID: 214951  Cd Length: 80  Bit Score: 72.85  E-value: 4.93e-16
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 62955755      4 SCCVLNCRHRtHERRNGVRFFHFPTwkqgygpqvseLSKRRRMAWIAAVRLKHISFNNIPRHMVVCSRHFHKGKPAYEML 83
Cdd:smart00980   1 KCCVPGCGNR-SKKNPGLSFFRFPK-----------EDPELRKKWLENLGLPDDPNRKPKKRSRICSRHFEPDDFDNSGR 68

                           90
                   ....*....|..
gi 62955755     84 ECDPDWAPSLHL 95
Cdd:smart00980  69 RLKPGAVPTLFL 80
HTH_Tnp_4 pfam13613
Helix-turn-helix of DDE superfamily endonuclease; This domain is the probable DNA-binding ...
 257-309 2.27e-14

Helix-turn-helix of DDE superfamily endonuclease; This domain is the probable DNA-binding region of transposase enzymes, necessary for efficient DNA transposition. Most of the members derive from the IS superfamily IS5 and rather fewer from IS4.


:

Pssm-ID: 463934  Cd Length: 53  Bit Score: 67.16  E-value: 2.27e-14
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|...
gi 62955755   257 RKLSPFQMLILTLMRLKLNLPDQLFAHIFSVGRKTASSIFKETVSMMYVRLNP 309
Cdd:pfam13613   1 RKLSLEDQLLLTLVYLRLNLTFQDLAYRFGVSESTASRIFHKWLDLLAQRLPP 53
transpos_IS3 super family cl41295
IS3 family transposase;
195-224 6.59e-03

IS3 family transposase;


The actual alignment was detected with superfamily member NF033516:

Pssm-ID: 468052 [Multi-domain]  Cd Length: 369  Bit Score: 38.70  E-value: 6.59e-03
                         10        20        30
                 ....*....|....*....|....*....|
gi 62955755  195 RAEINRLLEENRRLKKELAQKTMDEDFFKE 224
Cdd:NF033516  51 KELLTPEEEENRRLKRELAELRLENEILKK 80
 
Name Accession Description Interval E-value
DDE_Tnp_4 pfam13359
DDE superfamily endonuclease; This family of proteins are related to pfam00665 and are ...
 339-498 2.94e-38

DDE superfamily endonuclease; This family of proteins are related to pfam00665 and are probably endonucleases of the DDE superfamily. Transposase proteins are necessary for efficient DNA transposition. This domain is a member of the DDE superfamily, which contain three carboxylate residues that are believed to be responsible for coordinating metal ions needed for catalysis. The catalytic activity of this enzyme involves DNA cleavage at a specific site followed by a strand transfer reaction.


Pssm-ID: 463855 [Multi-domain]  Cd Length: 158  Bit Score: 137.02  E-value: 2.94e-38
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 62955755   339 VDSIEIDIDRVSNGKPKAQMVPQNKK--TLKYLICSIPRGAITFISKPRESQVDDKSLIESSGLLKKIQ----SGDLVLS 412
Cdd:pfam13359   1 VDGTEIPIQRPSNLEARGQTYSNYKHhnTLKVLIVVTPNGKIIFVSKGYPGSVSDKSILENSGLLKALNlafgPGDYILA 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 62955755   413 ECGFDIRDGVGLTCAEVDEGQLKVRDVETTRQISNLRLHVKRVTACVRTKYTFLNGPVLVSMtvpdvgedITFLDKIVTV 492
Cdd:pfam13359  81 DKGFPISDWLLVPYKKPGGAQLSPEEVEFNRRIASARIHVERVIGRLKGRFRILRGRLPISL--------PERLDKIVTV 152


                  ....*.
gi 62955755   493 CCALTN 498
Cdd:pfam13359 153 CCVLHN 158
THAP smart00980
The THAP domain is a putative DNA-binding domain (DBD) and probably also binds a zinc ion; It ...
 4-95 4.93e-16

The THAP domain is a putative DNA-binding domain (DBD) and probably also binds a zinc ion; It features the conserved C2CH architecture (consensus sequence: Cys - 2-4 residues - Cys - 35-50 residues - Cys - 2 residues - His). Other universal features include the location of the domain at the N-termini of proteins, its size of about 90 residues, a C-terminal AVPTIF box and several other conserved residues. Orthologues of the human THAP domain have been identified in other vertebrates and probably worms and flies, but not in other eukaryotes or any prokaryotes.


Pssm-ID: 214951  Cd Length: 80  Bit Score: 72.85  E-value: 4.93e-16
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 62955755      4 SCCVLNCRHRtHERRNGVRFFHFPTwkqgygpqvseLSKRRRMAWIAAVRLKHISFNNIPRHMVVCSRHFHKGKPAYEML 83
Cdd:smart00980   1 KCCVPGCGNR-SKKNPGLSFFRFPK-----------EDPELRKKWLENLGLPDDPNRKPKKRSRICSRHFEPDDFDNSGR 68

                           90
                   ....*....|..
gi 62955755     84 ECDPDWAPSLHL 95
Cdd:smart00980  69 RLKPGAVPTLFL 80
HTH_Tnp_4 pfam13613
Helix-turn-helix of DDE superfamily endonuclease; This domain is the probable DNA-binding ...
 257-309 2.27e-14

Helix-turn-helix of DDE superfamily endonuclease; This domain is the probable DNA-binding region of transposase enzymes, necessary for efficient DNA transposition. Most of the members derive from the IS superfamily IS5 and rather fewer from IS4.


Pssm-ID: 463934  Cd Length: 53  Bit Score: 67.16  E-value: 2.27e-14
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|...
gi 62955755   257 RKLSPFQMLILTLMRLKLNLPDQLFAHIFSVGRKTASSIFKETVSMMYVRLNP 309
Cdd:pfam13613   1 RKLSLEDQLLLTLVYLRLNLTFQDLAYRFGVSESTASRIFHKWLDLLAQRLPP 53
THAP pfam05485
THAP domain; The THAP domain is a putative DNA-binding domain (DBD) and probably also binds a ...
 5-94 4.96e-08

THAP domain; The THAP domain is a putative DNA-binding domain (DBD) and probably also binds a zinc ion. It features the conserved C2CH architecture (consensus sequence: Cys - 2-4 residues - Cys - 35-50 residues - Cys - 2 residues - His). Other universal features include the location of the domain at the N-termini of proteins, its size of about 90 residues, a C-terminal AVPTIF box and several other conserved residues. Orthologues of the human THAP domain have been identified in other vertebrates and probably worms and flies, but not in other eukaryotes or any prokaryotes.


Pssm-ID: 461662  Cd Length: 76  Bit Score: 50.22  E-value: 4.96e-08
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 62955755     5 CCVLNCRHRtHERRNGVRFFHFPtwkqgygpqvseLSKRRRMAWIAAVRLKHisfNNIPRHMVVCSRHFHKG--KPAYEM 82
Cdd:pfam05485   1 CSVPGCTNR-KKKNPRTSFHKFP------------KDPERRKKWLNACKRKD---LPPPSNSYVCSLHFEENdfEKSGGK 64

                          90
                  ....*....|..
gi 62955755    83 LECDPDWAPSLH 94
Cdd:pfam05485  65 RKLKPGAIPTLF 76
transpos_IS3 NF033516
IS3 family transposase;
195-224 6.59e-03

IS3 family transposase;


Pssm-ID: 468052 [Multi-domain]  Cd Length: 369  Bit Score: 38.70  E-value: 6.59e-03
                         10        20        30
                 ....*....|....*....|....*....|
gi 62955755  195 RAEINRLLEENRRLKKELAQKTMDEDFFKE 224
Cdd:NF033516  51 KELLTPEEEENRRLKRELAELRLENEILKK 80
 
Name Accession Description Interval E-value
DDE_Tnp_4 pfam13359
DDE superfamily endonuclease; This family of proteins are related to pfam00665 and are ...
 339-498 2.94e-38

DDE superfamily endonuclease; This family of proteins are related to pfam00665 and are probably endonucleases of the DDE superfamily. Transposase proteins are necessary for efficient DNA transposition. This domain is a member of the DDE superfamily, which contain three carboxylate residues that are believed to be responsible for coordinating metal ions needed for catalysis. The catalytic activity of this enzyme involves DNA cleavage at a specific site followed by a strand transfer reaction.


Pssm-ID: 463855 [Multi-domain]  Cd Length: 158  Bit Score: 137.02  E-value: 2.94e-38
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 62955755   339 VDSIEIDIDRVSNGKPKAQMVPQNKK--TLKYLICSIPRGAITFISKPRESQVDDKSLIESSGLLKKIQ----SGDLVLS 412
Cdd:pfam13359   1 VDGTEIPIQRPSNLEARGQTYSNYKHhnTLKVLIVVTPNGKIIFVSKGYPGSVSDKSILENSGLLKALNlafgPGDYILA 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 62955755   413 ECGFDIRDGVGLTCAEVDEGQLKVRDVETTRQISNLRLHVKRVTACVRTKYTFLNGPVLVSMtvpdvgedITFLDKIVTV 492
Cdd:pfam13359  81 DKGFPISDWLLVPYKKPGGAQLSPEEVEFNRRIASARIHVERVIGRLKGRFRILRGRLPISL--------PERLDKIVTV 152


                  ....*.
gi 62955755   493 CCALTN 498
Cdd:pfam13359 153 CCVLHN 158
THAP smart00980
The THAP domain is a putative DNA-binding domain (DBD) and probably also binds a zinc ion; It ...
 4-95 4.93e-16

The THAP domain is a putative DNA-binding domain (DBD) and probably also binds a zinc ion; It features the conserved C2CH architecture (consensus sequence: Cys - 2-4 residues - Cys - 35-50 residues - Cys - 2 residues - His). Other universal features include the location of the domain at the N-termini of proteins, its size of about 90 residues, a C-terminal AVPTIF box and several other conserved residues. Orthologues of the human THAP domain have been identified in other vertebrates and probably worms and flies, but not in other eukaryotes or any prokaryotes.


Pssm-ID: 214951  Cd Length: 80  Bit Score: 72.85  E-value: 4.93e-16
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 62955755      4 SCCVLNCRHRtHERRNGVRFFHFPTwkqgygpqvseLSKRRRMAWIAAVRLKHISFNNIPRHMVVCSRHFHKGKPAYEML 83
Cdd:smart00980   1 KCCVPGCGNR-SKKNPGLSFFRFPK-----------EDPELRKKWLENLGLPDDPNRKPKKRSRICSRHFEPDDFDNSGR 68

                           90
                   ....*....|..
gi 62955755     84 ECDPDWAPSLHL 95
Cdd:smart00980  69 RLKPGAVPTLFL 80
HTH_Tnp_4 pfam13613
Helix-turn-helix of DDE superfamily endonuclease; This domain is the probable DNA-binding ...
 257-309 2.27e-14

Helix-turn-helix of DDE superfamily endonuclease; This domain is the probable DNA-binding region of transposase enzymes, necessary for efficient DNA transposition. Most of the members derive from the IS superfamily IS5 and rather fewer from IS4.


Pssm-ID: 463934  Cd Length: 53  Bit Score: 67.16  E-value: 2.27e-14
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|...
gi 62955755   257 RKLSPFQMLILTLMRLKLNLPDQLFAHIFSVGRKTASSIFKETVSMMYVRLNP 309
Cdd:pfam13613   1 RKLSLEDQLLLTLVYLRLNLTFQDLAYRFGVSESTASRIFHKWLDLLAQRLPP 53
THAP pfam05485
THAP domain; The THAP domain is a putative DNA-binding domain (DBD) and probably also binds a ...
 5-94 4.96e-08

THAP domain; The THAP domain is a putative DNA-binding domain (DBD) and probably also binds a zinc ion. It features the conserved C2CH architecture (consensus sequence: Cys - 2-4 residues - Cys - 35-50 residues - Cys - 2 residues - His). Other universal features include the location of the domain at the N-termini of proteins, its size of about 90 residues, a C-terminal AVPTIF box and several other conserved residues. Orthologues of the human THAP domain have been identified in other vertebrates and probably worms and flies, but not in other eukaryotes or any prokaryotes.


Pssm-ID: 461662  Cd Length: 76  Bit Score: 50.22  E-value: 4.96e-08
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 62955755     5 CCVLNCRHRtHERRNGVRFFHFPtwkqgygpqvseLSKRRRMAWIAAVRLKHisfNNIPRHMVVCSRHFHKG--KPAYEM 82
Cdd:pfam05485   1 CSVPGCTNR-KKKNPRTSFHKFP------------KDPERRKKWLNACKRKD---LPPPSNSYVCSLHFEENdfEKSGGK 64

                          90
                  ....*....|..
gi 62955755    83 LECDPDWAPSLH 94
Cdd:pfam05485  65 RKLKPGAIPTLF 76
transpos_IS3 NF033516
IS3 family transposase;
195-224 6.59e-03

IS3 family transposase;


Pssm-ID: 468052 [Multi-domain]  Cd Length: 369  Bit Score: 38.70  E-value: 6.59e-03
                         10        20        30
                 ....*....|....*....|....*....|
gi 62955755  195 RAEINRLLEENRRLKKELAQKTMDEDFFKE 224
Cdd:NF033516  51 KELLTPEEEENRRLKRELAELRLENEILKK 80
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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