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Conserved domains on  [gi|2462522647|ref|XP_054223508|]
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splicing factor 3B subunit 2 isoform X2 [Homo sapiens]

Protein Classification

SAP domain-containing protein( domain architecture ID 13670223)

SAP (SAF-A/B, Acinus and PIAS) domain-containing protein may bind DNA or RNA and act as a transcriptional regulator

Graphical summary

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List of domain hits

Name Accession Description Interval E-value
DUF382 pfam04037
Domain of unknown function (DUF382); This domain is specific to the human splicing factor 3b ...
470-595 3.04e-85

Domain of unknown function (DUF382); This domain is specific to the human splicing factor 3b subunit 2 and it's orthologues. Splicing factor 3b subunit 2 or SAP145 is a suppressor of U2 snRNA mutations. Pre-mRNA splicing is catalyzed by a large ribonucleoprotein complex called the spliceosome. Spliceosomes are multi-component enzymes that catalyze pre-mRNA splicing and form step-wise by the ordered interaction of UsnRNPs and non-snRNP proteins with short conserved regions of the pre-mRNA at the 5' and 3' splice sites and branch site.


:

Pssm-ID: 461140  Cd Length: 126  Bit Score: 268.22  E-value: 3.04e-85
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462522647 470 RPDVVEMHDVTAQDPKLLVHLKATRNSVPVPRHWCFKRKYLQGKRGIEKPPFELPDFIKRTGIQEMREALQEKEEQKTMK 549
Cdd:pfam04037   1 RPEVVEWYDVDAPDPYLLVHLKSSRNTVPVPSHWSQKRKYLSGKRGIEKPPFQLPDFIEDTGIEEMRDALLEKEDEKSLK 80
                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*.
gi 2462522647 550 SKMREKVRPKMGKIDIDYQKLHDAFFKWQTKPKLTIHGDLYYEGKE 595
Cdd:pfam04037  81 QKQRERVQPKMGKLDIDYQKLHDAFFKFQTKPRLTPFGDLYYEGKE 126
CUS1 super family cl34931
Splicing factor 3b, subunit 2 [RNA processing and modification];
441-705 1.88e-83

Splicing factor 3b, subunit 2 [RNA processing and modification];


The actual alignment was detected with superfamily member COG5182:

Pssm-ID: 227509 [Multi-domain]  Cd Length: 429  Bit Score: 274.94  E-value: 1.88e-83
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462522647 441 KKPEAPKLSKKKLRRMNRFTVAELKQLVARPDVVEMHDVTAQDPKLLVHLKATRNSVPVPRHWCFKRKYLQGKRGIEKPP 520
Cdd:COG5182   116 KPYREESLSRQKKRKALQHRYEQLKLVVPYPEIFEWEDATCPDPMSLNRMKGCSNGVPVPRHWRSKSRYLSGHGYHKPRP 195
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462522647 521 FELPDFIKRTGIQEMREALQEKEEQKTMKSKMREKVRPKMGKIDIDYQKLHDAFFKWQTKPKLTIHGDLYYEGKEFETRL 600
Cdd:COG5182   196 FELPRHIIGTGIPQMRRMMREREAGMSLRERIRERVQPKMGGLDVDYRKLHDAFFSLGPKPYLSKFGEFYEEVDNDYRFV 275
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462522647 601 KEKKPGDLSDELRISLGMPVGpnahkVPPPWLIAMQRYGPPPSYPNLKIPGLN-SPIP-ESCSFGYHAGGWGKPPVDETG 678
Cdd:COG5182   276 KKKRPGAISAELREALGIDSG-----TPPPWLFNMQKHGMPPSYPDLKIPGLNwAPIPlEGDVYGYQPPGWHEPLFEVGP 350
                         250       260
                  ....*....|....*....|....*..
gi 2462522647 679 KPLYGDVFGTNAAEFqtktEEEEIDRT 705
Cdd:COG5182   351 ETAEGELLQFEAEEI----YNDEEQYT 373
SAP pfam02037
SAP domain; The SAP (after SAF-A/B, Acinus and PIAS) motif is a putative DNA/RNA binding ...
24-57 1.53e-05

SAP domain; The SAP (after SAF-A/B, Acinus and PIAS) motif is a putative DNA/RNA binding domain found in diverse nuclear and cytoplasmic proteins.


:

Pssm-ID: 460424 [Multi-domain]  Cd Length: 35  Bit Score: 42.39  E-value: 1.53e-05
                          10        20        30
                  ....*....|....*....|....*....|....
gi 2462522647  24 YGAWAAQELQAKLAEIGAPIQGNREELVERLQSY 57
Cdd:pfam02037   1 LSKLTVAELKEELRKRGLPTSGKKAELIERLQEY 34
 
Name Accession Description Interval E-value
DUF382 pfam04037
Domain of unknown function (DUF382); This domain is specific to the human splicing factor 3b ...
470-595 3.04e-85

Domain of unknown function (DUF382); This domain is specific to the human splicing factor 3b subunit 2 and it's orthologues. Splicing factor 3b subunit 2 or SAP145 is a suppressor of U2 snRNA mutations. Pre-mRNA splicing is catalyzed by a large ribonucleoprotein complex called the spliceosome. Spliceosomes are multi-component enzymes that catalyze pre-mRNA splicing and form step-wise by the ordered interaction of UsnRNPs and non-snRNP proteins with short conserved regions of the pre-mRNA at the 5' and 3' splice sites and branch site.


Pssm-ID: 461140  Cd Length: 126  Bit Score: 268.22  E-value: 3.04e-85
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462522647 470 RPDVVEMHDVTAQDPKLLVHLKATRNSVPVPRHWCFKRKYLQGKRGIEKPPFELPDFIKRTGIQEMREALQEKEEQKTMK 549
Cdd:pfam04037   1 RPEVVEWYDVDAPDPYLLVHLKSSRNTVPVPSHWSQKRKYLSGKRGIEKPPFQLPDFIEDTGIEEMRDALLEKEDEKSLK 80
                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*.
gi 2462522647 550 SKMREKVRPKMGKIDIDYQKLHDAFFKWQTKPKLTIHGDLYYEGKE 595
Cdd:pfam04037  81 QKQRERVQPKMGKLDIDYQKLHDAFFKFQTKPRLTPFGDLYYEGKE 126
CUS1 COG5182
Splicing factor 3b, subunit 2 [RNA processing and modification];
441-705 1.88e-83

Splicing factor 3b, subunit 2 [RNA processing and modification];


Pssm-ID: 227509 [Multi-domain]  Cd Length: 429  Bit Score: 274.94  E-value: 1.88e-83
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462522647 441 KKPEAPKLSKKKLRRMNRFTVAELKQLVARPDVVEMHDVTAQDPKLLVHLKATRNSVPVPRHWCFKRKYLQGKRGIEKPP 520
Cdd:COG5182   116 KPYREESLSRQKKRKALQHRYEQLKLVVPYPEIFEWEDATCPDPMSLNRMKGCSNGVPVPRHWRSKSRYLSGHGYHKPRP 195
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462522647 521 FELPDFIKRTGIQEMREALQEKEEQKTMKSKMREKVRPKMGKIDIDYQKLHDAFFKWQTKPKLTIHGDLYYEGKEFETRL 600
Cdd:COG5182   196 FELPRHIIGTGIPQMRRMMREREAGMSLRERIRERVQPKMGGLDVDYRKLHDAFFSLGPKPYLSKFGEFYEEVDNDYRFV 275
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462522647 601 KEKKPGDLSDELRISLGMPVGpnahkVPPPWLIAMQRYGPPPSYPNLKIPGLN-SPIP-ESCSFGYHAGGWGKPPVDETG 678
Cdd:COG5182   276 KKKRPGAISAELREALGIDSG-----TPPPWLFNMQKHGMPPSYPDLKIPGLNwAPIPlEGDVYGYQPPGWHEPLFEVGP 350
                         250       260
                  ....*....|....*....|....*..
gi 2462522647 679 KPLYGDVFGTNAAEFqtktEEEEIDRT 705
Cdd:COG5182   351 ETAEGELLQFEAEEI----YNDEEQYT 373
PSP smart00581
proline-rich domain in spliceosome associated proteins;
600-658 3.07e-23

proline-rich domain in spliceosome associated proteins;


Pssm-ID: 128850  Cd Length: 54  Bit Score: 93.19  E-value: 3.07e-23
                           10        20        30        40        50
                   ....*....|....*....|....*....|....*....|....*....|....*....
gi 2462522647  600 LKEKKPGDLSDELRISLGMPVGpnahkVPPPWLIAMQRYGPPPSYPNLKIPGLNSPIPE 658
Cdd:smart00581   1 FKHFKPGRISDELREALGLPPG-----QPPPWLYRMRRLGYPPGYPRLKIPGLNAPIPL 54
PSP pfam04046
PSP; Proline rich domain found in numerous spliceosome associated proteins.
604-653 4.77e-21

PSP; Proline rich domain found in numerous spliceosome associated proteins.


Pssm-ID: 461145  Cd Length: 46  Bit Score: 86.73  E-value: 4.77e-21
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|
gi 2462522647 604 KPGDLSDELRISLGMPVGpnahkVPPPWLIAMQRYGPPPSYPNLKIPGLN 653
Cdd:pfam04046   1 KPGKLSDELREALGLPEG-----QPPPWLYRMQRLGPPPSYPNLKIPGLN 45
SAP pfam02037
SAP domain; The SAP (after SAF-A/B, Acinus and PIAS) motif is a putative DNA/RNA binding ...
24-57 1.53e-05

SAP domain; The SAP (after SAF-A/B, Acinus and PIAS) motif is a putative DNA/RNA binding domain found in diverse nuclear and cytoplasmic proteins.


Pssm-ID: 460424 [Multi-domain]  Cd Length: 35  Bit Score: 42.39  E-value: 1.53e-05
                          10        20        30
                  ....*....|....*....|....*....|....
gi 2462522647  24 YGAWAAQELQAKLAEIGAPIQGNREELVERLQSY 57
Cdd:pfam02037   1 LSKLTVAELKEELRKRGLPTSGKKAELIERLQEY 34
SAP smart00513
Putative DNA-binding (bihelical) motif predicted to be involved in chromosomal organisation;
24-58 8.05e-05

Putative DNA-binding (bihelical) motif predicted to be involved in chromosomal organisation;


Pssm-ID: 128789 [Multi-domain]  Cd Length: 35  Bit Score: 40.55  E-value: 8.05e-05
                           10        20        30
                   ....*....|....*....|....*....|....*
gi 2462522647   24 YGAWAAQELQAKLAEIGAPIQGNREELVERLQSYT 58
Cdd:smart00513   1 LAKLKVSELKDELKKRGLSTSGTKAELVDRLLEAL 35
 
Name Accession Description Interval E-value
DUF382 pfam04037
Domain of unknown function (DUF382); This domain is specific to the human splicing factor 3b ...
470-595 3.04e-85

Domain of unknown function (DUF382); This domain is specific to the human splicing factor 3b subunit 2 and it's orthologues. Splicing factor 3b subunit 2 or SAP145 is a suppressor of U2 snRNA mutations. Pre-mRNA splicing is catalyzed by a large ribonucleoprotein complex called the spliceosome. Spliceosomes are multi-component enzymes that catalyze pre-mRNA splicing and form step-wise by the ordered interaction of UsnRNPs and non-snRNP proteins with short conserved regions of the pre-mRNA at the 5' and 3' splice sites and branch site.


Pssm-ID: 461140  Cd Length: 126  Bit Score: 268.22  E-value: 3.04e-85
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462522647 470 RPDVVEMHDVTAQDPKLLVHLKATRNSVPVPRHWCFKRKYLQGKRGIEKPPFELPDFIKRTGIQEMREALQEKEEQKTMK 549
Cdd:pfam04037   1 RPEVVEWYDVDAPDPYLLVHLKSSRNTVPVPSHWSQKRKYLSGKRGIEKPPFQLPDFIEDTGIEEMRDALLEKEDEKSLK 80
                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*.
gi 2462522647 550 SKMREKVRPKMGKIDIDYQKLHDAFFKWQTKPKLTIHGDLYYEGKE 595
Cdd:pfam04037  81 QKQRERVQPKMGKLDIDYQKLHDAFFKFQTKPRLTPFGDLYYEGKE 126
CUS1 COG5182
Splicing factor 3b, subunit 2 [RNA processing and modification];
441-705 1.88e-83

Splicing factor 3b, subunit 2 [RNA processing and modification];


Pssm-ID: 227509 [Multi-domain]  Cd Length: 429  Bit Score: 274.94  E-value: 1.88e-83
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462522647 441 KKPEAPKLSKKKLRRMNRFTVAELKQLVARPDVVEMHDVTAQDPKLLVHLKATRNSVPVPRHWCFKRKYLQGKRGIEKPP 520
Cdd:COG5182   116 KPYREESLSRQKKRKALQHRYEQLKLVVPYPEIFEWEDATCPDPMSLNRMKGCSNGVPVPRHWRSKSRYLSGHGYHKPRP 195
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462522647 521 FELPDFIKRTGIQEMREALQEKEEQKTMKSKMREKVRPKMGKIDIDYQKLHDAFFKWQTKPKLTIHGDLYYEGKEFETRL 600
Cdd:COG5182   196 FELPRHIIGTGIPQMRRMMREREAGMSLRERIRERVQPKMGGLDVDYRKLHDAFFSLGPKPYLSKFGEFYEEVDNDYRFV 275
                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462522647 601 KEKKPGDLSDELRISLGMPVGpnahkVPPPWLIAMQRYGPPPSYPNLKIPGLN-SPIP-ESCSFGYHAGGWGKPPVDETG 678
Cdd:COG5182   276 KKKRPGAISAELREALGIDSG-----TPPPWLFNMQKHGMPPSYPDLKIPGLNwAPIPlEGDVYGYQPPGWHEPLFEVGP 350
                         250       260
                  ....*....|....*....|....*..
gi 2462522647 679 KPLYGDVFGTNAAEFqtktEEEEIDRT 705
Cdd:COG5182   351 ETAEGELLQFEAEEI----YNDEEQYT 373
PSP smart00581
proline-rich domain in spliceosome associated proteins;
600-658 3.07e-23

proline-rich domain in spliceosome associated proteins;


Pssm-ID: 128850  Cd Length: 54  Bit Score: 93.19  E-value: 3.07e-23
                           10        20        30        40        50
                   ....*....|....*....|....*....|....*....|....*....|....*....
gi 2462522647  600 LKEKKPGDLSDELRISLGMPVGpnahkVPPPWLIAMQRYGPPPSYPNLKIPGLNSPIPE 658
Cdd:smart00581   1 FKHFKPGRISDELREALGLPPG-----QPPPWLYRMRRLGYPPGYPRLKIPGLNAPIPL 54
PSP pfam04046
PSP; Proline rich domain found in numerous spliceosome associated proteins.
604-653 4.77e-21

PSP; Proline rich domain found in numerous spliceosome associated proteins.


Pssm-ID: 461145  Cd Length: 46  Bit Score: 86.73  E-value: 4.77e-21
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|
gi 2462522647 604 KPGDLSDELRISLGMPVGpnahkVPPPWLIAMQRYGPPPSYPNLKIPGLN 653
Cdd:pfam04046   1 KPGKLSDELREALGLPEG-----QPPPWLYRMQRLGPPPSYPNLKIPGLN 45
SAP pfam02037
SAP domain; The SAP (after SAF-A/B, Acinus and PIAS) motif is a putative DNA/RNA binding ...
24-57 1.53e-05

SAP domain; The SAP (after SAF-A/B, Acinus and PIAS) motif is a putative DNA/RNA binding domain found in diverse nuclear and cytoplasmic proteins.


Pssm-ID: 460424 [Multi-domain]  Cd Length: 35  Bit Score: 42.39  E-value: 1.53e-05
                          10        20        30
                  ....*....|....*....|....*....|....
gi 2462522647  24 YGAWAAQELQAKLAEIGAPIQGNREELVERLQSY 57
Cdd:pfam02037   1 LSKLTVAELKEELRKRGLPTSGKKAELIERLQEY 34
SAP smart00513
Putative DNA-binding (bihelical) motif predicted to be involved in chromosomal organisation;
24-58 8.05e-05

Putative DNA-binding (bihelical) motif predicted to be involved in chromosomal organisation;


Pssm-ID: 128789 [Multi-domain]  Cd Length: 35  Bit Score: 40.55  E-value: 8.05e-05
                           10        20        30
                   ....*....|....*....|....*....|....*
gi 2462522647   24 YGAWAAQELQAKLAEIGAPIQGNREELVERLQSYT 58
Cdd:smart00513   1 LAKLKVSELKDELKKRGLSTSGTKAELVDRLLEAL 35
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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