Putative adipose-regulatory protein (Seipin); Seipin is a protein of approximately 400 residues, in humans, which is the product of a gene homologous to the murine guanine nucleotide-binding protein (G protein) gamma-3 linked gene. This gene is implicated in the regulation of body fat distribution and insulin resistance and particularly in the auto-immune disease Berardinelli-Seip congenital lipodystrophy type 2. Seipin has no similarity with other known proteins or consensus motifs that might predict its function, but it is predicted to contain two transmembrane domains at residues 28-49 and 237-258, in human, and a third transmembrane domain might be present at residues 155-173. Seipin may also be implicated in Silver spastic paraplegia syndrome and distal hereditary motor neuropathy type V.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2312341291  42 WIAVFLYGSFYYSYMPTVSYVSPVHYTFRTDCGspgpdlcsfPIANISLVKNNRdrVLMYGQPYRISLELELPESPVNQE 121
Cdd:pfam06775   5 VLSVVAYGLFYYAYVPEPVLSRPLHFQYGTGSN---------PYATVSLTSRAS--LLPPGQPYDVSVELTLPESPYNLE 73

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2312341291 122 LGMFMVVMSCYTKGGRIVSSTTRSTMLHYRSGLLQTLDTLVFAGLFFLGFAEQKQMVEVELYSDYREDSYVPTVGAVIEI 201
Cdd:pfam06775  74 LGNFMVRLELLSSNGKVLASSRRPAMLPYRSPLVRLLRTLLLLPPYLLGLREESQTLRVPMFESVVEGRENPPTSARVEI 153

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|..
gi 2312341291 202 QT-KRIQIYGAQLRIHAHFTGLRYLLYNFPVTSAILGVASNF 242
Cdd:pfam06775 154 QSaGLLQIYSAELIFEARLPGLRYLMYNWPITSFVVGTALFW 195

Putative adipose-regulatory protein (Seipin); Seipin is a protein of approximately 400 residues, in humans, which is the product of a gene homologous to the murine guanine nucleotide-binding protein (G protein) gamma-3 linked gene. This gene is implicated in the regulation of body fat distribution and insulin resistance and particularly in the auto-immune disease Berardinelli-Seip congenital lipodystrophy type 2. Seipin has no similarity with other known proteins or consensus motifs that might predict its function, but it is predicted to contain two transmembrane domains at residues 28-49 and 237-258, in human, and a third transmembrane domain might be present at residues 155-173. Seipin may also be implicated in Silver spastic paraplegia syndrome and distal hereditary motor neuropathy type V.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2312341291  42 WIAVFLYGSFYYSYMPTVSYVSPVHYTFRTDCGspgpdlcsfPIANISLVKNNRdrVLMYGQPYRISLELELPESPVNQE 121
Cdd:pfam06775   5 VLSVVAYGLFYYAYVPEPVLSRPLHFQYGTGSN---------PYATVSLTSRAS--LLPPGQPYDVSVELTLPESPYNLE 73

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2312341291 122 LGMFMVVMSCYTKGGRIVSSTTRSTMLHYRSGLLQTLDTLVFAGLFFLGFAEQKQMVEVELYSDYREDSYVPTVGAVIEI 201
Cdd:pfam06775  74 LGNFMVRLELLSSNGKVLASSRRPAMLPYRSPLVRLLRTLLLLPPYLLGLREESQTLRVPMFESVVEGRENPPTSARVEI 153

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|..
gi 2312341291 202 QT-KRIQIYGAQLRIHAHFTGLRYLLYNFPVTSAILGVASNF 242
Cdd:pfam06775 154 QSaGLLQIYSAELIFEARLPGLRYLMYNWPITSFVVGTALFW 195

Putative adipose-regulatory protein (Seipin); Seipin is a protein of approximately 400 residues, in humans, which is the product of a gene homologous to the murine guanine nucleotide-binding protein (G protein) gamma-3 linked gene. This gene is implicated in the regulation of body fat distribution and insulin resistance and particularly in the auto-immune disease Berardinelli-Seip congenital lipodystrophy type 2. Seipin has no similarity with other known proteins or consensus motifs that might predict its function, but it is predicted to contain two transmembrane domains at residues 28-49 and 237-258, in human, and a third transmembrane domain might be present at residues 155-173. Seipin may also be implicated in Silver spastic paraplegia syndrome and distal hereditary motor neuropathy type V.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2312341291  42 WIAVFLYGSFYYSYMPTVSYVSPVHYTFRTDCGspgpdlcsfPIANISLVKNNRdrVLMYGQPYRISLELELPESPVNQE 121
Cdd:pfam06775   5 VLSVVAYGLFYYAYVPEPVLSRPLHFQYGTGSN---------PYATVSLTSRAS--LLPPGQPYDVSVELTLPESPYNLE 73

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2312341291 122 LGMFMVVMSCYTKGGRIVSSTTRSTMLHYRSGLLQTLDTLVFAGLFFLGFAEQKQMVEVELYSDYREDSYVPTVGAVIEI 201
Cdd:pfam06775  74 LGNFMVRLELLSSNGKVLASSRRPAMLPYRSPLVRLLRTLLLLPPYLLGLREESQTLRVPMFESVVEGRENPPTSARVEI 153

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|..
gi 2312341291 202 QT-KRIQIYGAQLRIHAHFTGLRYLLYNFPVTSAILGVASNF 242
Cdd:pfam06775 154 QSaGLLQIYSAELIFEARLPGLRYLMYNWPITSFVVGTALFW 195

Seipin and similar proteins; Seipin is a homo-oligomeric integral membrane protein in the endoplasmic reticulum (ER) that concentrates at junctions with cytoplasmic lipid droplets (LDs). It acts as a cell-autonomous regulator of lipolysis essential for adipocyte differentiation. Seipin is predicted to contain two transmembrane domains at N-terminus and C-terminus, respectively. Human seipin, also called Bernardinelli-Seip congenital lipodystrophy type 2 protein (BSCL2), may contain a third transmembrane domain in the middle region. Mutations in the seipin gene underlie human congenital generalized lipodystrophy. Seipin may also be implicated in Silver spastic paraplegia syndrome and distal hereditary motor neuropathy type V. Seipin homologs from fungi, plants, and insects are also included in this family. There are three SEIPIN homologs in Arabidopsis thaliana, designated SEIPIN1, SEIPIN2, and SEIPIN3. Saccharomyces cerevisiae Seipin (Sei1) is also called few lipid droplets protein 1 (Fld1p). Similar to their animal homologs, plant and yeast seipins also play roles in lipid droplet (LD) biogenesis. Human seipin exists as an undecamer, and this oligomerization state is critical for its physiological function. In contrast to Human seipin, S. cerevisiae Sei1-mediated LD formation depends on Ldb16, a yeast specific binding partner. Sei1 forms a homodecameric ring that scaffolds and positions Ldb16.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2312341291  57 PTVSYVSPVHYTFRTDCGSPGPdlCSFPIANISLVKNNRDRVLMYGQPYRISLELELPESPVNQELGMFMVVMSCYTKGG 136
Cdd:cd23993     1 PSNSHPLPVHNGVQTCLETSTP--CTFPGAKVSLTKKSIPVGDMMGQKYDVNLQLYCPESPQNDHLGMFNVLIDVYRGPD 78

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2312341291 137 RIVSSTTRSTMLHYRSGLLQTLDTLVFAGLFFLGFAEQKQMVEVELYSDYREDSYVPTVGAVIEIQTKRIQIYGAQLRIH 216
Cdd:cd23993    79 EKIFHSSRSIMCLYRSDLMSMQETEVQSGLSRLGVYEEEQLNTVEIEDKYSEESSYPTTSVFLEIESAQIQLYIHPLGIK 158

                  ....*
gi 2312341291 217 AHFTG 221
Cdd:cd23993   159 ARFTG 163