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Conserved domains on  [gi|2217349969|ref|XP_047305949|]
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major facilitator superfamily domain-containing protein 8 isoform X3 [Homo sapiens]

Protein Classification

MFSD8-like MFS transporter( domain architecture ID 13023588)

MFSD8-like major facilitator superfamily (MFS) transporter similar to MFS domain-containing protein 8, also called ceroid-lipofuscinosis neuronal protein 7 (CLN7), a polytopic lysosomal membrane protein that may transport small solutes by using chemiosmotic ion gradients

CATH:  1.20.1250.20
Gene Ontology:  GO:0022857|GO:0055085|GO:0016020
PubMed:  26098515|26758938|26234418
SCOP:  3000310
TCDB:  2.A.1

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
MFS_MFSD8 cd17326
Major facilitator superfamily domain-containing protein 8; Major facilitator superfamily (MFS) ...
 1-395 2.18e-130

Major facilitator superfamily domain-containing protein 8; Major facilitator superfamily (MFS) domain-containing protein 8 (MFSD8) is also called ceroid-lipofuscinosis neuronal protein 7 (CLN7). It is a polytopic lysosomal membrane protein that may transport small solutes by using chemiosmotic ion gradients. Mutations in MFSD8/CLN7 cause a variant of late-infantile neuronal ceroid lipofuscinoses (vLINCL), a neurodegenerative lysosomal storage disorder. Some variants are associated with nonsyndromic autosomal recessive macular dystrophy. MFSD8/CLN7 belongs to the Eukaryotic Solute carrier 46 (SLC46)/Bacterial Tetracycline resistance -like (SLC46/TetA-like) family of the Major Facilitator Superfamily (MFS) of transporters. MFS proteins are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.


:

Pssm-ID: 340884 [Multi-domain]  Cd Length: 374  Bit Score: 379.63  E-value: 2.18e-130
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2217349969   1 MFLSSVGFSVVMMSIWPYLQKIDPTADTSFLGWVIASYSLGQMVASPIFGLWSNYRP-RKEPLIVSILISVAANCLYAYL 79
Cdd:cd17326     8 MFLSSLQFSIVLTSIWPYLQKIDPTATASFFGWVVAAYSLGQLVFSPVFGYWSNKTSsVKEPLIVSILIAIVGNILYALA 87

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2217349969  80 HIPASHNKYYMLVARGLLGIGAGNVAVVRSYTAGATSLQERTSSMANISMCQALGFILGPA-----------STDEAQVP 148
Cdd:cd17326    88 EYFPSNRKYVMLVARFLIGVGAGNVAVLRAYVAAASTLKDRTRAMAVISAAQALGFIVGPAlqaaftpigypGYVIGIKL 167

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2217349969 149 QGNIDQVAV--------------------VAINVLFFVTLFIFALFETIITPLTMDMYAWTQEQAVLYNGIILAALGVEA 208
Cdd:cd17326   168 RFNMYTAPAwlmailgiiniililfffkeVVCIFLFFVVLFIFANLETIGTPLTMDMYGWTREEAVLYNGIILAVSGVLS 247

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2217349969 209 VVIFLGVKLLSKK-IGERAILLGGLIVVWVGFFILLPWGnqfpkiqwedlhnnsipnttfgeiiiglwkspmeddnerpt 287
Cdd:cd17326   248 VLVFLLFKFLSLGlIDERKILLGGLILFFLGFVVTLPWG----------------------------------------- 286

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2217349969 288 gcsieqawclytpvihlaqfLTSAVLIGLGYPVCNLMSYTLYSKILGPKPQGVYMGWLTASGSGARILGPMFISQVYAHW 367
Cdd:cd17326   287 --------------------ITGVILFGLGYPIGNVMLYTLYSKILGPKPQGTMQGVLTASGSLARTLGPIFVTAIYTHF 346

                         410       420
                  ....*....|....*....|....*...
gi 2217349969 368 GPRWAFSLVCGIIVLTITLLGVVYKRLI 395
Cdd:cd17326   347 GPRWTWIITLGLLLLAIILLIVFYKRLV 374
 
Name Accession Description Interval E-value
MFS_MFSD8 cd17326
Major facilitator superfamily domain-containing protein 8; Major facilitator superfamily (MFS) ...
 1-395 2.18e-130

Major facilitator superfamily domain-containing protein 8; Major facilitator superfamily (MFS) domain-containing protein 8 (MFSD8) is also called ceroid-lipofuscinosis neuronal protein 7 (CLN7). It is a polytopic lysosomal membrane protein that may transport small solutes by using chemiosmotic ion gradients. Mutations in MFSD8/CLN7 cause a variant of late-infantile neuronal ceroid lipofuscinoses (vLINCL), a neurodegenerative lysosomal storage disorder. Some variants are associated with nonsyndromic autosomal recessive macular dystrophy. MFSD8/CLN7 belongs to the Eukaryotic Solute carrier 46 (SLC46)/Bacterial Tetracycline resistance -like (SLC46/TetA-like) family of the Major Facilitator Superfamily (MFS) of transporters. MFS proteins are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.


Pssm-ID: 340884 [Multi-domain]  Cd Length: 374  Bit Score: 379.63  E-value: 2.18e-130
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2217349969   1 MFLSSVGFSVVMMSIWPYLQKIDPTADTSFLGWVIASYSLGQMVASPIFGLWSNYRP-RKEPLIVSILISVAANCLYAYL 79
Cdd:cd17326     8 MFLSSLQFSIVLTSIWPYLQKIDPTATASFFGWVVAAYSLGQLVFSPVFGYWSNKTSsVKEPLIVSILIAIVGNILYALA 87

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2217349969  80 HIPASHNKYYMLVARGLLGIGAGNVAVVRSYTAGATSLQERTSSMANISMCQALGFILGPA-----------STDEAQVP 148
Cdd:cd17326    88 EYFPSNRKYVMLVARFLIGVGAGNVAVLRAYVAAASTLKDRTRAMAVISAAQALGFIVGPAlqaaftpigypGYVIGIKL 167

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2217349969 149 QGNIDQVAV--------------------VAINVLFFVTLFIFALFETIITPLTMDMYAWTQEQAVLYNGIILAALGVEA 208
Cdd:cd17326   168 RFNMYTAPAwlmailgiiniililfffkeVVCIFLFFVVLFIFANLETIGTPLTMDMYGWTREEAVLYNGIILAVSGVLS 247

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2217349969 209 VVIFLGVKLLSKK-IGERAILLGGLIVVWVGFFILLPWGnqfpkiqwedlhnnsipnttfgeiiiglwkspmeddnerpt 287
Cdd:cd17326   248 VLVFLLFKFLSLGlIDERKILLGGLILFFLGFVVTLPWG----------------------------------------- 286

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2217349969 288 gcsieqawclytpvihlaqfLTSAVLIGLGYPVCNLMSYTLYSKILGPKPQGVYMGWLTASGSGARILGPMFISQVYAHW 367
Cdd:cd17326   287 --------------------ITGVILFGLGYPIGNVMLYTLYSKILGPKPQGTMQGVLTASGSLARTLGPIFVTAIYTHF 346

                         410       420
                  ....*....|....*....|....*...
gi 2217349969 368 GPRWAFSLVCGIIVLTITLLGVVYKRLI 395
Cdd:cd17326   347 GPRWTWIITLGLLLLAIILLIVFYKRLV 374
AraJ COG2814
Predicted arabinose efflux permease AraJ, MFS family [Carbohydrate transport and metabolism];
1-247 3.23e-13

Predicted arabinose efflux permease AraJ, MFS family [Carbohydrate transport and metabolism];


Pssm-ID: 442063 [Multi-domain]  Cd Length: 348  Bit Score: 70.39  E-value: 3.23e-13
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2217349969   1 MFLSSVGFSVVMmSIWPYLQKiDPTADTSFLGWVIASYSLGQMVASPIFGLWSNYRPRKEPLIVSILISVAANCLYAylh 80
Cdd:COG2814    19 AFLSGLGIGIVL-PALPLIAA-DLGASPAQAGLVVTAYLLGAALGAPLAGRLADRFGRRRVLLLGLLLFALGSLLCA--- 93

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2217349969  81 ipASHNKYYMLVARGLLGIGAG-NVAVVRSYTAGATSLQERTSSMANISMCQALGFILGPA------------------- 140
Cdd:COG2814    94 --LAPSLWLLLAARFLQGLGAGaLFPAALALIADLVPPERRGRALGLLGAGLGLGPALGPLlgglladlfgwrwvflvna 171

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2217349969 141 -----------------STDEAQVPQGNIDQV----AVVAINVLFFVTLFIFALFETIITPLTMDMYAWTQEQAvlynGI 199
Cdd:COG2814   172 vlallalllllrllpesRPAARARLRGSLRELlrrpRLLLLLLLAFLLGFGFFALFTYLPLYLQEVLGLSASAA----GL 247

                         250       260       270       280
                  ....*....|....*....|....*....|....*....|....*...
gi 2217349969 200 ILAALGVEAVVIFLGVKLLSKKIGERAILLGGLIVVWVGFFILLPWGN 247
Cdd:COG2814   248 LLALFGLGGVLGALLAGRLADRFGRRRLLLIGLLLLALGLLLLALAGS 295
MFS_1 pfam07690
Major Facilitator Superfamily;
1-357 1.76e-09

Major Facilitator Superfamily;


Pssm-ID: 429598 [Multi-domain]  Cd Length: 344  Bit Score: 58.97  E-value: 1.76e-09
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2217349969   1 MFLSSVGFSVVMMSIWPYLQKiDPTADTSFLGWVIASYSLGQMVASPIFGLWSNYRPRKEPLIVSILISVAANCLYAYlh 80
Cdd:pfam07690   5 AFLAALGRSLLGPALPLLLAE-DLGISPTEIGLLLTLFSLGYALAQPLAGRLSDRFGRRRVLLIGLLLFALGLLLLLF-- 81

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2217349969  81 ipaSHNKYYMLVARGLLGIGAGNV-AVVRSYTAGATSLQERTSSMANISMCQALGFILGPAStdeAQVPQGNIDQVAVVA 159
Cdd:pfam07690  82 ---ASSLWLLLVLRVLQGLGAGALfPAALALIADWFPPEERGRALGLVSAGFGLGAALGPLL---GGLLASLFGWRAAFL 155

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2217349969 160 INVLFFVTLFIFALF--------------ETIITPLTMDMYAWTQEQAVLYNGIILAALGVEAVVIFLGVKLLSKKIGER 225
Cdd:pfam07690 156 ILAILSLLAAVLLLLprpppeskrpkpaeEARLSLIVAWKALLRDPVLWLLLALLLFGFAFFGLLTYLPLYQEVLGLSAL 235

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2217349969 226 AILLGGLIVVWVGFFILLPWGNQFPKIQwedlhNNSIPNTTFGEIIIGLWkspmeddnerptgcsieqAWCLYTPVIHLA 305
Cdd:pfam07690 236 LAGLLLGLGGLLGAIGRLLLGRLSDRLG-----RRRRLLLALLLLILAAL------------------GLLLLSLTLSSL 292

                         330       340       350       360       370
                  ....*....|....*....|....*....|....*....|....*....|..
gi 2217349969 306 QFLTSAVLIGLGYPVCNLMSYTLYSKILGPKPQGVYMGWLTASGSGARILGP 357
Cdd:pfam07690 293 WLLLALLLLGFGFGLVFPALNALVSDLAPKEERGTASGLYNTAGSLGGALGP 344
 
Name Accession Description Interval E-value
MFS_MFSD8 cd17326
Major facilitator superfamily domain-containing protein 8; Major facilitator superfamily (MFS) ...
 1-395 2.18e-130

Major facilitator superfamily domain-containing protein 8; Major facilitator superfamily (MFS) domain-containing protein 8 (MFSD8) is also called ceroid-lipofuscinosis neuronal protein 7 (CLN7). It is a polytopic lysosomal membrane protein that may transport small solutes by using chemiosmotic ion gradients. Mutations in MFSD8/CLN7 cause a variant of late-infantile neuronal ceroid lipofuscinoses (vLINCL), a neurodegenerative lysosomal storage disorder. Some variants are associated with nonsyndromic autosomal recessive macular dystrophy. MFSD8/CLN7 belongs to the Eukaryotic Solute carrier 46 (SLC46)/Bacterial Tetracycline resistance -like (SLC46/TetA-like) family of the Major Facilitator Superfamily (MFS) of transporters. MFS proteins are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.


Pssm-ID: 340884 [Multi-domain]  Cd Length: 374  Bit Score: 379.63  E-value: 2.18e-130
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2217349969   1 MFLSSVGFSVVMMSIWPYLQKIDPTADTSFLGWVIASYSLGQMVASPIFGLWSNYRP-RKEPLIVSILISVAANCLYAYL 79
Cdd:cd17326     8 MFLSSLQFSIVLTSIWPYLQKIDPTATASFFGWVVAAYSLGQLVFSPVFGYWSNKTSsVKEPLIVSILIAIVGNILYALA 87

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2217349969  80 HIPASHNKYYMLVARGLLGIGAGNVAVVRSYTAGATSLQERTSSMANISMCQALGFILGPA-----------STDEAQVP 148
Cdd:cd17326    88 EYFPSNRKYVMLVARFLIGVGAGNVAVLRAYVAAASTLKDRTRAMAVISAAQALGFIVGPAlqaaftpigypGYVIGIKL 167

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2217349969 149 QGNIDQVAV--------------------VAINVLFFVTLFIFALFETIITPLTMDMYAWTQEQAVLYNGIILAALGVEA 208
Cdd:cd17326   168 RFNMYTAPAwlmailgiiniililfffkeVVCIFLFFVVLFIFANLETIGTPLTMDMYGWTREEAVLYNGIILAVSGVLS 247

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2217349969 209 VVIFLGVKLLSKK-IGERAILLGGLIVVWVGFFILLPWGnqfpkiqwedlhnnsipnttfgeiiiglwkspmeddnerpt 287
Cdd:cd17326   248 VLVFLLFKFLSLGlIDERKILLGGLILFFLGFVVTLPWG----------------------------------------- 286

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2217349969 288 gcsieqawclytpvihlaqfLTSAVLIGLGYPVCNLMSYTLYSKILGPKPQGVYMGWLTASGSGARILGPMFISQVYAHW 367
Cdd:cd17326   287 --------------------ITGVILFGLGYPIGNVMLYTLYSKILGPKPQGTMQGVLTASGSLARTLGPIFVTAIYTHF 346

                         410       420
                  ....*....|....*....|....*...
gi 2217349969 368 GPRWAFSLVCGIIVLTITLLGVVYKRLI 395
Cdd:cd17326   347 GPRWTWIITLGLLLLAIILLIVFYKRLV 374
MFS_SLC46_TetA_like cd17330
Eukaryotic Solute carrier 46 (SLC46) family, Bacterial Tetracycline resistance proteins, and ...
 1-389 1.37e-54

Eukaryotic Solute carrier 46 (SLC46) family, Bacterial Tetracycline resistance proteins, and similar proteins of the Major Facilitator Superfamily of transporters; This family is composed of the eukaryotic proteins MFSD9, MFSD10, MFSD14, and SLC46 family proteins, as well as bacterial multidrug resistance (MDR) transporters such as tetracycline resistance protein TetA and multidrug resistance protein MdtG. MDR transporters are drug/H+ antiporters (DHA) that mediate the efflux of a variety of drugs and toxic compounds, and confer resistance to these compounds. TetA proteins confer resistance to tetracycline while MdtG confers resistance to fosfomycin and deoxycholate. The Solute carrier 46 (SLC46) family is composed of three vertebrate members (SLC46A1, SLC46A2, and SLC46A3), the best-studied of which is SLC46A1, which functions both as an intestinal proton-coupled high-affinity folate transporter involved in the absorption of folates and as an intestinal heme transporter which mediates heme uptake. MFSD10 facilitates the uptake of organic anions such as some non-steroidal anti-inflammatory drugs (NSAIDs) and confers resistance to such NSAIDs. The SLC46/TetA-like family belongs to the Major Facilitator Superfamily (MFS) of membrane transport proteins, which are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.


Pssm-ID: 340888 [Multi-domain]  Cd Length: 349  Bit Score: 184.32  E-value: 1.37e-54
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2217349969   1 MFLSSVGFSVVMMSIWPYLQKI-DPTADTSFLGWVIASYSLGQMVASPIFGLWSNYRPRKEPLIVSILISVAANCLYAYl 79
Cdd:cd17330     5 VFLDALGFGIVFPVLPFYVREFnKLGASGYYVGLLQSSYSLGQFLASPLWGRLSDRYGRKPVLLLSLAGSAVSYVLLGL- 83

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2217349969  80 hipaSHNKYYMLVARGLLGIGAGNVAVVRSYTAGATSLQERTSSMANISMCQALGFILGPASTDEAQVPQGNIDQVAVVA 159
Cdd:cd17330    84 ----SRNLWLLFLARFLDGLTGGNVSVAQAYIADITDEEERAKAFGIISAAFGLGFILGPALGGFLSTPYGYALPFLVAA 159

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2217349969 160 ----------------INVLFFVTLFI---FALFETIITPLTMDMYAWTQEQAvlynGIILAALGVEAVVIFLGV-KLLS 219
Cdd:cd17330   160 alallnlvlvlfflppLRRLLLVYFLIalaFAGLESVFSLFLKDRFGFSPSQI----GLLLALVGIAGILAQLLLiPRLA 235

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2217349969 220 KKIGERAILLGGLIVVWVGFFiLLPWGNQFPkiqwedlhnnsipnttfgeiiiglwkspmeddnerptgcsieqawclyt 299
Cdd:cd17330   236 KRLGERKLLRLGLLLAIIGLL-LLPFASSVP------------------------------------------------- 265

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2217349969 300 pvihlaQFLTSAVLIGLGYPVCNLMSYTLYSKILGPKPQGVYMGWLTASGSGARILGPMFISQVYAHWGPRWAFSLVCGI 379
Cdd:cd17330   266 ------LLVLAAAILAFGTGIVFPALSALLSKSVPSKEQGKAQGIIQSLQSLARIIGPLLAGPLYSFLGPGVPFLLAAGL 339

                         410
                  ....*....|
gi 2217349969 380 IVLTITLLGV 389
Cdd:cd17330   340 LLIALFLLSL 349
MFS_MdtG_SLC18_like cd17325
bacterial MdtG-like and eukaryotic solute carrier 18 (SLC18) family of the Major Facilitator ...
 1-392 1.42e-15

bacterial MdtG-like and eukaryotic solute carrier 18 (SLC18) family of the Major Facilitator Superfamily of transporters; This family is composed of eukaryotic solute carrier 18 (SLC18) family transporters and related bacterial multidrug resistance (MDR) transporters including several proteins from Escherichia coli such as multidrug resistance protein MdtG, from Bacillus subtilis such as multidrug resistance proteins 1 (Bmr1) and 2 (Bmr2), and from Staphylococcus aureus such as quinolone resistance protein NorA. The family also includes Escherichia coli arabinose efflux transporters YfcJ and YhhS. MDR transporters are drug/H+ antiporters (DHA) that mediate the efflux of a variety of drugs and toxic compounds, and confer resistance to these compounds. The SLC18 transporter family includes vesicular monoamine transporters (VAT1 and VAT2), vesicular acetylcholine transporter (VAChT), and SLC18B1, which is proposed to be a vesicular polyamine transporter (VPAT). The MdtG/SLC18 family belongs to the Major Facilitator Superfamily (MFS) of membrane transport proteins, which are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.


Pssm-ID: 340883 [Multi-domain]  Cd Length: 375  Bit Score: 77.62  E-value: 1.42e-15
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2217349969   1 MFLSSVGFSVVMMSIWPYLQKIDptADTSFLGWVIASYSLGQMVASPIFGLWSNYRPRKEPLIVSILISVAANCLYAYLH 80
Cdd:cd17325     5 LFLDMLGYGIIIPILPLYAESLG--ASVAQIGLIVAAYALAQLLFSPPAGRLSDRFGRKRLLLLGLLLLAVSTLLFAFAT 82

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2217349969  81 IPashnkYYMLVARGLLGIGAGNVAVVR-SYTAGATSLQERTSSMANISMCQALGFILGPA------------------- 140
Cdd:cd17325    83 SY-----WQLLLARFLQGLASAAVWPAAmALIADIAPPEERGRAMGIFSSAIGLGFLIGPLlgglladalgyrapflvca 157

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2217349969 141 ------------------STDEAQVPQGNIDQV---------AVVAINVLFFVTLFIFALFETIITPLTMDMYAWTQEQA 193
Cdd:cd17325   158 alallalvlallllpeprPPPNKRAVSAARLRSglrlllrdrRLLALFLAIFVLAFAFGALEPFLPLYAAELGGLSPAQI 237

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2217349969 194 vlynGIILAALGVEAVVIFLGVKLLSKKIGERAILLGGLIVVWVGFFillpwgnqfpkiqwedlhnnsipnttfgeiiig 273
Cdd:cd17325   238 ----GLLFGAQGLASALSQPPAGKLSDRIGRKPLILIGLLLSAVALL--------------------------------- 280

                         330       340       350       360       370       380       390       400
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2217349969 274 lwkspmeddnerptgcsieqawcLYTPVIHLAQFLTSAVLIGLGYPVCNLMSYTLYSKILGPKPQGVYMGWLTASGSGAR 353
Cdd:cd17325   281 -----------------------LLPLATSFWLLLLLLALLGLGLGLVFPATLALLADIVPPEGRGTAMGLFNTAFSLGM 337

                         410       420       430
                  ....*....|....*....|....*....|....*....
gi 2217349969 354 ILGPMFISQVYAHWGPRWAFsLVCGIIVLTITLLGVVYK 392
Cdd:cd17325   338 VLGPLLGGFLYDAFGFATPF-LAAAALLLLAAVLFLLLR 375
AraJ COG2814
Predicted arabinose efflux permease AraJ, MFS family [Carbohydrate transport and metabolism];
1-247 3.23e-13

Predicted arabinose efflux permease AraJ, MFS family [Carbohydrate transport and metabolism];


Pssm-ID: 442063 [Multi-domain]  Cd Length: 348  Bit Score: 70.39  E-value: 3.23e-13
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2217349969   1 MFLSSVGFSVVMmSIWPYLQKiDPTADTSFLGWVIASYSLGQMVASPIFGLWSNYRPRKEPLIVSILISVAANCLYAylh 80
Cdd:COG2814    19 AFLSGLGIGIVL-PALPLIAA-DLGASPAQAGLVVTAYLLGAALGAPLAGRLADRFGRRRVLLLGLLLFALGSLLCA--- 93

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2217349969  81 ipASHNKYYMLVARGLLGIGAG-NVAVVRSYTAGATSLQERTSSMANISMCQALGFILGPA------------------- 140
Cdd:COG2814    94 --LAPSLWLLLAARFLQGLGAGaLFPAALALIADLVPPERRGRALGLLGAGLGLGPALGPLlgglladlfgwrwvflvna 171

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2217349969 141 -----------------STDEAQVPQGNIDQV----AVVAINVLFFVTLFIFALFETIITPLTMDMYAWTQEQAvlynGI 199
Cdd:COG2814   172 vlallalllllrllpesRPAARARLRGSLRELlrrpRLLLLLLLAFLLGFGFFALFTYLPLYLQEVLGLSASAA----GL 247

                         250       260       270       280
                  ....*....|....*....|....*....|....*....|....*...
gi 2217349969 200 ILAALGVEAVVIFLGVKLLSKKIGERAILLGGLIVVWVGFFILLPWGN 247
Cdd:COG2814   248 LLALFGLGGVLGALLAGRLADRFGRRRLLLIGLLLLALGLLLLALAGS 295
MFS_MFSD9 cd17390
Major facilitator superfamily domain-containing protein 9; Major facilitator superfamily ...
 2-140 1.68e-10

Major facilitator superfamily domain-containing protein 9; Major facilitator superfamily domain-containing protein 9 (MFSD9) is expressed in the central nervous system (CNS) and in most peripheral tissues but at very low expression levels. The function of MFSD9 is unknown. MFSD9 belongs to the Eukaryotic Solute carrier 46 (SLC46)/Bacterial Tetracycline resistance (TetA) -like (SLC46/TetA-like) family of the Major Facilitator Superfamily (MFS) of transporters. MFS proteins are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.


Pssm-ID: 340948 [Multi-domain]  Cd Length: 350  Bit Score: 61.86  E-value: 1.68e-10
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2217349969   2 FLSSVGFSVVMMSIWPYLQKIDptADTSFLGWVIASYSLGQMVASPIFGLWSNYRPRKEPLIVSILIsvaanCLYAYLHI 81
Cdd:cd17390     6 FLDLFGVSMILPLLSTHIRSLG--ASPTVIGLIGSVYGALQLFSSPLVGSWSDVVGRRPVLLICLLL-----TALGYVLL 78

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|....*....
gi 2217349969  82 PASHNKYYMLVARGLLGIGAGNVAVVRSYTAGATSLQERTSSMANISMCQALGFILGPA 140
Cdd:cd17390    79 GFSTSLALLFLSRIPLGIFKHTQSLCKAYLSDLVSEQERSRVLGRFNAASSLGFILGPV 137
MFS_1 pfam07690
Major Facilitator Superfamily;
1-357 1.76e-09

Major Facilitator Superfamily;


Pssm-ID: 429598 [Multi-domain]  Cd Length: 344  Bit Score: 58.97  E-value: 1.76e-09
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2217349969   1 MFLSSVGFSVVMMSIWPYLQKiDPTADTSFLGWVIASYSLGQMVASPIFGLWSNYRPRKEPLIVSILISVAANCLYAYlh 80
Cdd:pfam07690   5 AFLAALGRSLLGPALPLLLAE-DLGISPTEIGLLLTLFSLGYALAQPLAGRLSDRFGRRRVLLIGLLLFALGLLLLLF-- 81

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2217349969  81 ipaSHNKYYMLVARGLLGIGAGNV-AVVRSYTAGATSLQERTSSMANISMCQALGFILGPAStdeAQVPQGNIDQVAVVA 159
Cdd:pfam07690  82 ---ASSLWLLLVLRVLQGLGAGALfPAALALIADWFPPEERGRALGLVSAGFGLGAALGPLL---GGLLASLFGWRAAFL 155

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2217349969 160 INVLFFVTLFIFALF--------------ETIITPLTMDMYAWTQEQAVLYNGIILAALGVEAVVIFLGVKLLSKKIGER 225
Cdd:pfam07690 156 ILAILSLLAAVLLLLprpppeskrpkpaeEARLSLIVAWKALLRDPVLWLLLALLLFGFAFFGLLTYLPLYQEVLGLSAL 235

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2217349969 226 AILLGGLIVVWVGFFILLPWGNQFPKIQwedlhNNSIPNTTFGEIIIGLWkspmeddnerptgcsieqAWCLYTPVIHLA 305
Cdd:pfam07690 236 LAGLLLGLGGLLGAIGRLLLGRLSDRLG-----RRRRLLLALLLLILAAL------------------GLLLLSLTLSSL 292

                         330       340       350       360       370
                  ....*....|....*....|....*....|....*....|....*....|..
gi 2217349969 306 QFLTSAVLIGLGYPVCNLMSYTLYSKILGPKPQGVYMGWLTASGSGARILGP 357
Cdd:pfam07690 293 WLLLALLLLGFGFGLVFPALNALVSDLAPKEERGTASGLYNTAGSLGGALGP 344
MFS_MFSD10 cd17389
Major facilitator superfamily domain-containing protein 10; Major facilitator superfamily ...
 31-181 2.47e-08

Major facilitator superfamily domain-containing protein 10; Major facilitator superfamily domain-containing protein 10 (MFSD10) is also called tetracycline transporter-like protein (TETRAN). It is expressed in various human tissues, including the kidney. In cultured cells, its overexpression facilitated the uptake of organic anions such as some non-steroidal anti-inflammatory drugs (NSAIDs). MFSD10/TETRAN overexpression cause resistance to some NSAIDs, suggesting that it may be an organic anion transporter that serves as an efflux pump for some NSAIDs and various other organic anions at the final excretion step. MFSD10 belongs to the Eukaryotic Solute carrier 46 (SLC46)/Bacterial Tetracycline resistance (TetA) -like (SLC46/TetA-like) family of the Major Facilitator Superfamily (MFS) of transporters. MFS proteins are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.


Pssm-ID: 340947 [Multi-domain]  Cd Length: 391  Bit Score: 55.36  E-value: 2.47e-08
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2217349969  31 LGWVIAS-YSLGQMVASPIFGLWSNYRPRKEPLIVSILISVAANCLYAylhipASHNKYYMLVARGLLGIGAGNVAVVRS 109
Cdd:cd17389    65 FGGLIGSlFSLLQFLSSPITGAASDVYGRKPVLLLTMIGLAISYALWA-----VSRSFTIFLLSRIVGGISKGNVSLSTA 139

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 2217349969 110 YTAGATSLQERTSSMANISMCQALGFILGPA-----STDEAQVPQGNIDQVAVVAINVLFFVTLFIFALF-ETIITPL 181
Cdd:cd17389   140 IVADVTSPKKRSKGMALIGVAFSLGFIIGPMigayfASFTRSLGEVFFSMPALFALVLALADLLFIFFFLpETLPSNL 217
MFS_TetA cd17388
Tetracycline resistance protein TetA and related proteins of the Major Facilitator Superfamily ...
 2-242 1.50e-06

Tetracycline resistance protein TetA and related proteins of the Major Facilitator Superfamily of transporters; This subfamily is composed of tetracycline resistance proteins similar to Escherichia coli TetA(A), TetA(B), and TetA(E), which are metal-tetracycline/H(+) antiporters that confer resistance to tetracycline by an active tetracycline efflux, which is an energy-dependent process that decreases the accumulation of the antibiotic in cells. TetA-like tetracycline resistance proteins belongs to the Eukaryotic Solute carrier 46 (SLC46)/Bacterial Tetracycline resistance (TetA) -like (SLC46/TetA-like) family of the Major Facilitator Superfamily (MFS) of transporters. MFS proteins are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.


Pssm-ID: 340946 [Multi-domain]  Cd Length: 385  Bit Score: 49.94  E-value: 1.50e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2217349969   2 FLSSVGFSVvMMSIWPYL--QKIDPTAD-TSFLGWVIASYSLGQMVASPIFGLWSNYRPRKEPLIVSILISVAAnclyaY 78
Cdd:cd17388     9 LLDAMGIGL-IMPVLPTLlrEFVGDSADaATHYGVLLALYALMQFIFAPILGALSDRFGRRPVLLLSLAGAALD-----Y 82

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2217349969  79 LHIPASHNKYYMLVARGLLGIGAGNVAVVRSYTAGATSLQERTSSMANISMCQALGFILGPA------------------ 140
Cdd:cd17388    83 LLMAFAPTLWWLYLGRIIAGITGATGAVAAAYIADVTPPEQRARNFGLLGAAFGLGFIAGPAlggllgeisprapflvaa 162

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2217349969 141 -------------------------STDEAQVPQGNIDQVAVVAINVLFFVTLFIFALFETIITPL----TMDMYAWTQE 191
Cdd:cd17388   163 glnglnfllglfvlpeslpperrraFRWRRANPFGSLRWLTGFPGLLGLLAVFFLIQLIGQVPATTwvlfTEDRFDWSPT 242

                         250       260       270       280       290
                  ....*....|....*....|....*....|....*....|....*....|..
gi 2217349969 192 QavlyNGIILAALGVEAVVI-FLGVKLLSKKIGERAILLGGLIVVWVGFFIL 242
Cdd:cd17388   243 M----VGLSLAVFGLLHALFqAFVTGPIAKRLGEKRAILLGLAADALGFLLL 290
UhpC COG2271
Sugar phosphate permease [Carbohydrate transport and metabolism];
31-394 2.41e-06

Sugar phosphate permease [Carbohydrate transport and metabolism];


Pssm-ID: 441872 [Multi-domain]  Cd Length: 363  Bit Score: 49.10  E-value: 2.41e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2217349969  31 LGWVIASYSLGQMVASPIFGLWSNYRPRKEPLIVSILISVAANCLYAYlhipaSHNKYYMLVARGLLGIG-AGNVAVVRS 109
Cdd:COG2271    47 LGLLASAFFLGYALGQIPAGLLADRFGRRRVLAIGLLLWGLATLLFGF-----ATSFWQLLLLRFLLGLGeAGFFPAALK 121

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2217349969 110 YTAGATSLQERTSSMANISMCQALGFILGPASTdeaqvpQGNIDQ-------------------VAVVAINVLFFVTLFI 170
Cdd:COG2271   122 LIAEWFPPKERGRALGIFNAGGPLGGALAPPLL------GWLLAAfgwraaflilglpglllalLRFWLLALAYFLVYFA 195

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2217349969 171 FALFETIITPLTMDMYAWTQEQAVLYNGIILAAlgveAVVIFLGVKLLSKKIGERaillgGLIVVWVGFFILLPwgnqfp 250
Cdd:COG2271   196 LYGFLTWLPTYLVEVRGLSLAQAGLLLSLPFLA----GIVGSLLGGWLSDRLGRR-----RKLVLAIGLLLAAL------ 260

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2217349969 251 kiqwedlhnnsipnttfgeiiiglwkspmeddnerptgcsieqAWCLYTPVIHLAQFLTSAVLIGLGYPVCNLMSYTLYS 330
Cdd:COG2271   261 -------------------------------------------ALLLLALLPSPALAIALLFLAGFGLGGAFGLLWALAA 297

                         330       340       350       360       370       380
                  ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 2217349969 331 KILGPKPQGVYMGWLTASGSGARILGPMFISQVYAHWGPRWAFSLVCGIIVLTITLLGVVYKRL 394
Cdd:COG2271   298 ELFPKKARGTASGLVNTFGFLGGALGPLLVGYLLDATGYQAAFLLLAALALLAALLALLLLRET 361
Sugar_tr pfam00083
Sugar (and other) transporter;
26-102 4.66e-06

Sugar (and other) transporter;


Pssm-ID: 395036 [Multi-domain]  Cd Length: 452  Bit Score: 48.42  E-value: 4.66e-06
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 2217349969  26 ADTSFLGWVIASYSLGQMVASPIFGLWSNYRPRKEPLIVSILISVAANCLyayLHIPASHNKYYML-VARGLLGIGAG 102
Cdd:pfam00083  43 ALSVLSGLIVSIFSVGCFIGSLFAGKLGDRFGRKKSLLIANVLFVIGAVL---QGAAKGKWSVYQLiVGRVLVGIGVG 117
MFS_arabinose_efflux_permease_like cd17473
Putative arabinose efflux permease family transporters of the Major Facilitator Superfamily; ...
 17-242 1.99e-05

Putative arabinose efflux permease family transporters of the Major Facilitator Superfamily; This family includes a group of putative arabinose efflux permease family transporters, such as alpha proteobacterium quinolone resistance protein NorA (characterized Staphylococcus aureus Quinolone resistance protein NorA belongs to a different group), Desulfovibrio dechloracetivorans bacillibactin exporter, Vibrio aerogenes antiseptic resistance protein. The biological function of those transporters remain unclear. They belong to the Major Facilitator Superfamily (MFS) of membrane transport proteins, which are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.


Pssm-ID: 341026 [Multi-domain]  Cd Length: 374  Bit Score: 46.41  E-value: 1.99e-05
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2217349969  17 PYLQKIDPTADTSFLGWVIASYSLGQMVASPIFGLWSNYRPRKEPLIVSILISVAANCLYAYLhipashNKYY-MLVARG 95
Cdd:cd17473    23 PAIAQHFVPNSELLVPLLLTLPALFIALFAPLAGWLADRFGRKRLLIAGLLLYGIGGVAGLFL------DSLYaLLASRA 96

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2217349969  96 LLGIGAGNVAVVrsytagATSL-------QERTSSMANISMCQALG--------------------------FILGPAS- 141
Cdd:cd17473    97 LLGIGVAGIMTL------ATALigdyfegEERAKMLGLQSAAMSLGgvvflllggalaeigwrapflvyllaLPVLILVl 170

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2217349969 142 ---TDEAQVPQGNIDQVAVVA-------INVLFFVTLFIFALFETIITPLTMDMYAwTQEQAVLYNGIILAALGVEAVVI 211
Cdd:cd17473   171 lflPEPARKSKSAASPGAATAaalpwkrVLPIYFLAFLAMLLFYVIPIQLPFLLQE-RGGGSAALIGLALAIASLAGAIG 249

                         250       260       270
                  ....*....|....*....|....*....|.
gi 2217349969 212 FLGVKLLSKKIGERAILLGGLIVVWVGFFIL 242
Cdd:cd17473   250 ALLFGRLKRRLGKRRLLAIGFALMALGFLLL 280
ProP COG0477
MFS family permease, includes anhydromuropeptide permease AmpG [Carbohydrate transport and ...
 1-139 5.82e-05

MFS family permease, includes anhydromuropeptide permease AmpG [Carbohydrate transport and metabolism, Amino acid transport and metabolism, Inorganic ion transport and metabolism, General function prediction only];


Pssm-ID: 440245 [Multi-domain]  Cd Length: 295  Bit Score: 44.42  E-value: 5.82e-05
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2217349969   1 MFLSSVGFSVVMMSIwPYLQKiDPTADTSFLGWVIASYSLGQMVASPIFGLWSNYRPRKEPLIVSILISVAANCLYAYLH 80
Cdd:COG0477    23 TFLEGLDFTIVNVAL-PSIAA-DLGASSAQLGWIVSAYLLGRAIGLLLFGRLGDRYGRKRVLLIGLLLFGLASLLCGLAP 100

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|
gi 2217349969  81 IPAShnkyyMLVARGLLGIGAG-NVAVVRSYTAGATSLQERTSSMANISMCQALGFILGP 139
Cdd:COG0477   101 SPEL-----LIAARALQGIGAGgLMPGALALIAELFPARERGRALGLWGAAIGLGLALGP 155
MFS_Azr1_MDR_like cd17502
Saccharomyces cerevisiae Azole resistance protein 1 (Azr1p), and similar multidrug resistance ...
 27-243 9.03e-05

Saccharomyces cerevisiae Azole resistance protein 1 (Azr1p), and similar multidrug resistance (MDR) transporters of the Major Facilitator Superfamily; This subfamily is composed of multidrug resistance (MDR) transporters including various Saccharomyces cerevisiae proteins such as azole resistance protein 1 (Azr1p), vacuolar basic amino acid transporter 1 (Vba1p), vacuolar basic amino acid transporter 5 (Vba5p), and Sge1p (also known as Nor1p, 10-N-nonyl acridine orange resistance protein, and crystal violet resistance protein). MDR transporters are drug/H+ antiporters (DHA) that mediate the efflux of a variety of drugs and toxic compounds, and confer resistance to these compounds. This subfamily belongs to the Methylenomycin A resistance protein (also called MMR peptide) and similar multidrug resistance (MDR) transporters (MMR-like MDR transporter) family of the Major Facilitator Superfamily (MFS) of transporters. MFS proteins are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.


Pssm-ID: 341045 [Multi-domain]  Cd Length: 337  Bit Score: 44.09  E-value: 9.03e-05
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2217349969  27 DTSFLGWVIASYSLGQMVASPIFGLWSNYRPRKEPLIVSILISVAANCLYAylhipASHNKYYMLVARGLLGIGAGNVAV 106
Cdd:cd17502    28 GLSLVSWVGTAYLLTSTAFQPIYGKLSDLFGRKWVLLIAIVIFELGSLLCG-----LAPNMTMLIVGRAIQGIGGGGLMS 102

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2217349969 107 VrSYTAGA--TSLQERTSSMANISMCQALGFILGP----ASTDEA--------QVPQGNIDQVAVVAINVLFFVTLFIFA 172
Cdd:cd17502   103 L-AFIIIAdlVPPRERGKYQGYIGAVFGIASVAGPliggAFTDHLswrwcfyiNLPIGGLALALLRTILLSSIFGFLFGG 181

                         170       180       190       200       210       220       230
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 2217349969 173 LFETIITPLTMdMYAWTQEQAVLYNGIILAALGVEAVV-IFLGVKLLSKKIGERAILLGGLIVVWVGFFILL 243
Cdd:cd17502   182 ALFGLIYYLPL-YFQLVLGDSATKSGVRLLPLIIGLVIgSIISGALISKTGRYKPLIVIGTILMSVGAGLLT 252
MFS cd06174
Major Facilitator Superfamily; The Major Facilitator Superfamily (MFS) is a large and diverse ...
 26-174 1.32e-04

Major Facilitator Superfamily; The Major Facilitator Superfamily (MFS) is a large and diverse group of secondary transporters that includes uniporters, symporters, and antiporters. MFS proteins facilitate the transport across cytoplasmic or internal membranes of a variety of substrates including ions, sugar phosphates, drugs, neurotransmitters, nucleosides, amino acids, and peptides. They do so using the electrochemical potential of the transported substrates. Uniporters transport a single substrate, while symporters and antiporters transport two substrates in the same or in opposite directions, respectively, across membranes. MFS proteins are typically 400 to 600 amino acids in length, and the majority contain 12 transmembrane alpha helices (TMs) connected by hydrophilic loops. The N- and C-terminal halves of these proteins display weak similarity and may be the result of a gene duplication/fusion event. Based on kinetic studies and the structures of a few bacterial superfamily members, GlpT (glycerol-3-phosphate transporter), LacY (lactose permease), and EmrD (multidrug transporter), MFS proteins are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement. Bacterial members function primarily for nutrient uptake, and as drug-efflux pumps to confer antibiotic resistance. Some MFS proteins have medical significance in humans such as the glucose transporter Glut4, which is impaired in type II diabetes, and glucose-6-phosphate transporter (G6PT), which causes glycogen storage disease when mutated.


Pssm-ID: 349949 [Multi-domain]  Cd Length: 378  Bit Score: 43.95  E-value: 1.32e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2217349969  26 ADTSFLGWVIASYSLGQMVASPIFGLWSNYRPRKEPLIVSILISVAANCLYAYLHIPAShnkyyMLVARGLLGIGAGNVA 105
Cdd:cd06174    28 LSASQLGLLFALFSLGYALLQPLAGLLADRFGRRPVLLLGLLLFALGALLFAFAPSFWL-----LLLGRFLLGLGSGLID 102

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2217349969 106 VVR-SYTAGATSLQERTSSMANISMCQALGFILGPASTDEAqVPQGNIDQVAVVAINVLFFVTLFIFALF 174
Cdd:cd06174   103 PAVlALIADLFPERERGRALGLLQAFGSVGGILGPLLGGIL-ASSLGFGWRAVFLIAAALALLAAILLLL 171
MFS_NepI_like cd17324
Purine ribonucleoside efflux pump NepI and similar transporters of the Major Facilitator ...
 32-138 1.87e-04

Purine ribonucleoside efflux pump NepI and similar transporters of the Major Facilitator Superfamily; This family is composed of purine efflux pumps such as Escherichia coli NepI and Bacillus subtilis PbuE, sugar efflux transporters such as Corynebacterium glutamicum arabinose efflux permease, multidrug resistance (MDR) transporters such as Streptomyces lividans chloramphenicol resistance protein (CmlR), and similar proteins. NepI and PbuE are involved in the efflux of purine ribonucleosides such as guanosine, adenosine and inosine, as well as purine bases like guanine, adenine, and hypoxanthine, and purine base analogs. They play a role in the maintenance of cellular purine base pools, as well as in protecting the cells and conferring resistance against toxic purine base analogs such as 6-mercaptopurine. MDR transporters are drug/H+ antiporters (DHA) that mediate the efflux of a variety of drugs and toxic compounds, and confer resistance to these compounds. The NepI-like family belongs to the Major Facilitator Superfamily (MFS) of membrane transport proteins, which are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.


Pssm-ID: 340882 [Multi-domain]  Cd Length: 370  Bit Score: 43.31  E-value: 1.87e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2217349969  32 GWVIASYSLGQMVASPIFGLWSNYRPRKEPLIVSILISVAANCLYAylhipASHNKYYMLVARGLLGIGAGNV-AVVRSY 110
Cdd:cd17324    37 GLLVSAYALGYALGALLLGPLSDRIERRRLLLVLLLLFILGNLLAA-----LAPSFALLLLARALAGLAHGGFwAIAAAY 111

                          90       100
                  ....*....|....*....|....*...
gi 2217349969 111 TAGATSLQERTSSMANISMCQALGFILG 138
Cdd:cd17324   112 AADLVPPEKRGRAIGLVFSGLTLGLVLG 139
AraJ COG2814
Predicted arabinose efflux permease AraJ, MFS family [Carbohydrate transport and metabolism];
303-387 1.12e-03

Predicted arabinose efflux permease AraJ, MFS family [Carbohydrate transport and metabolism];


Pssm-ID: 442063 [Multi-domain]  Cd Length: 348  Bit Score: 40.73  E-value: 1.12e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2217349969 303 HLAQFLTSAVLIGLGYPVCNLMSYTLYSKILGPKPQGVYMGWLTASGSGARILGPMFISQVYAHWGPRWAFsLVCGIIVL 382
Cdd:COG2814    97 SLWLLLAARFLQGLGAGALFPAALALIADLVPPERRGRALGLLGAGLGLGPALGPLLGGLLADLFGWRWVF-LVNAVLAL 175


                  ....*
gi 2217349969 383 TITLL 387
Cdd:COG2814   176 LALLL 180
MFS_MMR_MDR_like cd17321
Methylenomycin A resistance protein (also called MMR peptide) and similar multidrug resistance ...
 1-275 1.19e-03

Methylenomycin A resistance protein (also called MMR peptide) and similar multidrug resistance (MDR) transporters of the Major Facilitator Superfamily; This family is composed of bacterial, fungal, and archaeal multidrug resistance (MDR) transporters including several proteins from Bacilli such as methylenomycin A resistance protein (also called MMR peptide), tetracycline resistance protein (TetB), and lincomycin resistance protein LmrB, as well as fungal proteins such as vacuolar basic amino acid transporters, which are involved in the transport into vacuoles of the basic amino acids histidine, lysine, and arginine in Saccharomyces cerevisiae, and aminotriazole/azole resistance proteins. MDR transporters are drug/H+ antiporters (DHA) that mediate the efflux of a variety of drugs and toxic compounds, and confer resistance to these compounds. For example, MMR confers resistance to the epoxide antibiotic methylenomycin while TetB resistance to tetracycline by an active tetracycline efflux. MMR-like MDR transporters belong to the Major Facilitator Superfamily (MFS) of membrane transport proteins, which are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.


Pssm-ID: 340879 [Multi-domain]  Cd Length: 370  Bit Score: 40.62  E-value: 1.19e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2217349969   1 MFLSSVGFSVVMMSIwPYLQKiDPTADTSFLGWVIASYSLGQMVASPIFGLWSNYRPRKEPLIVSILISVAANCLYAYlh 80
Cdd:cd17321     5 VFLAALDVTIVNVAL-PTIAR-DLGASPSQLQWVVNAYLLAFAALLLLAGRLGDRFGRKRVFLIGLALFGLASLLCAL-- 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2217349969  81 ipaSHNKYYMLVARGLLGIGAG-----NVAVVRSYTAGAtslqERTSSMANISMCQALGFILGPA------------STD 143
Cdd:cd17321    81 ---APSLAFLIAARALQGIGAAaimpaSLALIAATFPGE----RRGRALGLWGAVVGIGAALGPLlgglllelfgwrWIF 153

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2217349969 144 EAQVPqgnidqVAVVAI---------NVLFFVTLFIFALFETIITPLTMDMYAWTQEQAvlynGIILAALGVE-AVVIFL 213
Cdd:cd17321   154 LINVP------IGLVALllartfsgaVLAAFLLGAALGGLLFLLPLYLQGVLGYSPLQA----GLALLPLALAmLVAAPL 223

                         250       260       270       280       290       300
                  ....*....|....*....|....*....|....*....|....*....|....*....|..
gi 2217349969 214 GVKLLsKKIGERAILLGGLIVVWVGFFILLPWGnqfpkiqwedlHNNSIPNTTFGEIIIGLW 275
Cdd:cd17321   224 AGRLA-DRFGPRLVLVAGLLLTAVGLLLLALLG-----------ADSSVWLLLPGLVLLGLG 273
MFS_arabinose_efflux_permease_like cd17473
Putative arabinose efflux permease family transporters of the Major Facilitator Superfamily; ...
 1-173 2.28e-03

Putative arabinose efflux permease family transporters of the Major Facilitator Superfamily; This family includes a group of putative arabinose efflux permease family transporters, such as alpha proteobacterium quinolone resistance protein NorA (characterized Staphylococcus aureus Quinolone resistance protein NorA belongs to a different group), Desulfovibrio dechloracetivorans bacillibactin exporter, Vibrio aerogenes antiseptic resistance protein. The biological function of those transporters remain unclear. They belong to the Major Facilitator Superfamily (MFS) of membrane transport proteins, which are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.


Pssm-ID: 341026 [Multi-domain]  Cd Length: 374  Bit Score: 39.86  E-value: 2.28e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2217349969   1 MFLSSVGFSVVMMSIWPYLQKIDpTADTSFLGWVIASYSLGQMVASPIFGLWSNYRPrKEPLIVSILISVAAnclyAYLH 80
Cdd:cd17473   206 AFLAMLLFYVIPIQLPFLLQERG-GGSAALIGLALAIASLAGAIGALLFGRLKRRLG-KRRLLAIGFALMAL----GFLL 279

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2217349969  81 IPASHNKYYMLVARGLLGIGAG-NVAVVRSYTAGATSLQERTSSMANISMCQALGFILGPASTDEAQVPQGNIDQVAVVA 159
Cdd:cd17473   280 LALASGLWVVLLGAILAGLGFGlLLPTLNSWAMSLVPPELRGRAMGIVTSAFFLGQFLSPLVLGPLVNLTGGLSGAFLIL 359

                         170
                  ....*....|....
gi 2217349969 160 INVLFFVTLFIFAL 173
Cdd:cd17473   360 GVLALVLAIVLLLL 373
MFS_YfcJ_like cd17489
Escherichia coli YfcJ, YhhS, and similar transporters of the Major Facilitator Superfamily; ...
 297-390 2.87e-03

Escherichia coli YfcJ, YhhS, and similar transporters of the Major Facilitator Superfamily; This subfamily is composed of Escherichia coli membrane proteins, YfcJ and YhhS, Bacillus subtilis uncharacterized MFS-type transporter YwoG, and similar proteins. YfcJ and YhhS are putative arabinose efflux transporters. YhhS has been implicated glyphosate resistance. YfcJ-like arabinose efflux transporters belong to the bacterial MdtG-like and eukaryotic solute carrier 18 (SLC18) family of the Major Facilitator Superfamily (MFS) of transporters. MFS proteins are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.


Pssm-ID: 341042 [Multi-domain]  Cd Length: 367  Bit Score: 39.50  E-value: 2.87e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2217349969 297 LYTPVIHLAQFLTSAVLIGLGYPVCNLMSYTLYSKILGPKPQGVYMGWLTASGSGARILGPMFISQVYAHWGPRWAFSLV 376
Cdd:cd17489    77 LYLLATSVALLLVLRLIHGIGWGAFTTAAATLVADIIPPSRRGEGIGYYGLATTLAMALGPALGLFLYQHLGFAVLFIVA 156

                          90
                  ....*....|....
gi 2217349969 377 CGIIVLTITLLGVV 390
Cdd:cd17489   157 AVLALLALLLVFLV 170
MFS_YfcJ_like cd17489
Escherichia coli YfcJ, YhhS, and similar transporters of the Major Facilitator Superfamily; ...
 1-140 3.06e-03

Escherichia coli YfcJ, YhhS, and similar transporters of the Major Facilitator Superfamily; This subfamily is composed of Escherichia coli membrane proteins, YfcJ and YhhS, Bacillus subtilis uncharacterized MFS-type transporter YwoG, and similar proteins. YfcJ and YhhS are putative arabinose efflux transporters. YhhS has been implicated glyphosate resistance. YfcJ-like arabinose efflux transporters belong to the bacterial MdtG-like and eukaryotic solute carrier 18 (SLC18) family of the Major Facilitator Superfamily (MFS) of transporters. MFS proteins are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.


Pssm-ID: 341042 [Multi-domain]  Cd Length: 367  Bit Score: 39.50  E-value: 3.06e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2217349969   1 MFLSSVGFSVVMMSIWPYLQKIDPTADTSflGWVIASYSLGQMVASPIFGLWSNYRPRKEPLIVSILISVAANCLYAYLH 80
Cdd:cd17489     5 NFFFFLSFYLLLPVLPLYAHDLGGSEAVA--GLVVGLFTLAALLARPFAGRLLDRFGRKRLLLIGLLIFALATLLYLLAT 82

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|.
gi 2217349969  81 IPAShnkyyMLVARGLLGIGAGNVAVVRS-YTAGATSLQERTSSMANISMCQALGFILGPA 140
Cdd:cd17489    83 SVAL-----LLVLRLIHGIGWGAFTTAAAtLVADIIPPSRRGEGIGYYGLATTLAMALGPA 138
MFS_GLUT_like cd17315
Glucose transporters (GLUTs) and other similar sugar transporters of the Major Facilitator ...
 27-107 6.58e-03

Glucose transporters (GLUTs) and other similar sugar transporters of the Major Facilitator Superfamily; This family is composed of glucose transporters (GLUTs) and other sugar transporters including fungal hexose transporters (HXT), bacterial xylose transporter (XylE), plant sugar transport proteins (STP) and polyol transporters (PLT), H(+)-myo-inositol cotransporter (HMIT), and similar proteins. GLUTs, also called Solute carrier family 2, facilitated glucose transporters (SLC2A), are a family of proteins that facilitate the transport of hexoses such as glucose and fructose. There are fourteen GLUTs found in humans; they display different substrate specificities and tissue expression. They have been categorized into three classes based on sequence similarity: Class 1 (GLUTs 1-4, 14); Class 2 (GLUTs 5, 7, 9, and 11); and Class 3 (GLUTs 6, 8, 10, 12, and HMIT). GLUT proteins are comprised of about 500 amino acid residues, possess a single N-linked oligosaccharide, and have 12 transmembrane segments. The GLUT-like family belongs to the Major Facilitator Superfamily (MFS) of membrane transport proteins, which are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.


Pssm-ID: 340873 [Multi-domain]  Cd Length: 365  Bit Score: 38.32  E-value: 6.58e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2217349969  27 DTSFLGWVIASYSLGQMVASPIFGLWSNYRPRKEPLIVSILISVAANCLYAylhipASHNKYYMLVARGLLGIGAGNVAV 106
Cdd:cd17315    32 STSLQGLVVSSLLLGAAIGSLFGGPLADRFGRRKSLLIAAVLYVIGALLSA-----LAPNVWVLIVGRFLLGLGVGLASV 106


                  .
gi 2217349969 107 V 107
Cdd:cd17315   107 L 107
MFS_SLC46 cd17386
Solute carrier 46 (SLC46) family of the Major Facilitator Superfamily of transporters; The ...
 44-230 7.72e-03

Solute carrier 46 (SLC46) family of the Major Facilitator Superfamily of transporters; The solute carrier 46 (SLC46) family is composed of three vertebrate members (SLC46A1, SLC46A2, and SLC46A3) and similar proteins from insects and nematodes. The best-studied member is SLC46A1, also called proton-coupled folate transporter (PCFT), which functions both as an intestinal proton-coupled high-affinity folate transporter involved in the absorption of folates and as an intestinal heme transporter which mediates heme uptake. SLC46A2, also called thymic stromal cotransporter protein (TSCOT), is a putative 12-transmembrane protein mainly expressed in the thymic cortex in a specific thymic epithelial cell (TEC) subpopulation. SLC46A3 is a lysosomal membrane protein that functions as a direct transporter of noncleavable antibody maytansine-based catabolites from the lysosome to the cytoplasm. The SLC46 family belongs to the Eukaryotic Solute carrier 46 (SLC46)/Bacterial Tetracycline resistance (TetA) -like (SLC46/TetA-like) family of the Major Facilitator Superfamily (MFS) of transporters. MFS proteins are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.


Pssm-ID: 340944 [Multi-domain]  Cd Length: 360  Bit Score: 38.03  E-value: 7.72e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2217349969  44 VASPIFGLWSNYRPRKEPLIVSI---LISVAANCLYAYLHIPASHNKYYM-LVARGLLGIGAGNVAVVRSYTAGATSLQE 119
Cdd:cd17386    45 FSSLFLGSWSDRYGRKPPLLMSLvglLLSSLILVLIVYFTTVPSVWWFYLsAIPLALFGGFTAFLSASFAYISDISSEKK 124

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2217349969 120 RTSSMANISMCQALGFILGPASTDEAQVPQGNIDQVAVVA-------INVLFFVTLFIFALFETIITPLTMD-----MY- 186
Cdd:cd17386   125 RTYRMAILEVALGLGGFLGSLLSGHLFKALGYAWSFSISAalllialLYILFFLTLLILLLLAAFLTIFVLSgesdvLYl 204

                         170       180       190       200       210
                  ....*....|....*....|....*....|....*....|....*....|
gi 2217349969 187 ------AWTQEQAVLYNGiilAALGVEAVVIFLGVKLLSKKIGERAILLG 230
Cdd:cd17386   205 fvlekfAWNVKDFSYFST---LGSLIYLTGMLGGVLLLRCLLKMSDSTLA 251
MFS_MdtH_MDR_like cd17329
Multidrug resistance protein MdtH and similar multidrug resistance (MDR) transporters of the ...
 1-140 8.04e-03

Multidrug resistance protein MdtH and similar multidrug resistance (MDR) transporters of the Major Facilitator Superfamily; This family is composed of Escherichia coli MdtH and similar multidrug resistance (MDR) transporters from bacteria and archaea, many of which remain uncharacterized. MDR transporters are drug/H+ antiporters (DHA) that mediate the efflux of a variety of drugs and toxic compounds, and confer resistance to these compounds. MdtH confers resistance to norfloxacin and enoxacin. MdtH-like MDR transporters belong to the Major Facilitator Superfamily (MFS) of membrane transport proteins, which are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.


Pssm-ID: 340887 [Multi-domain]  Cd Length: 376  Bit Score: 37.98  E-value: 8.04e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2217349969   1 MFLSSVGFSVVMMSIWPYLQKiDPTADTSFLGWVIASYSLGQMVASPIFGLWSNYRPRKEPLIVSILISVAANCLYAYLH 80
Cdd:cd17329     8 SFLNRLGFFMVWPFMAIYLHQ-QLGLSASIVGLVLALSAVAGIVASLIGGRLADRFGRKPVMLAGLLLRALGFALLGFAH 86

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|...
gi 2217349969  81 IPAshnkyymLVARGLLGIGAGN---VAVVRSYTAGATSLQERTSSMANISMCQALGFILGPA 140
Cdd:cd17329    87 SPW-------LFAIALVLTGFGGglfEPASEAMIADVTTPENRTRAFSLLYWAINLGVAIGPL 142
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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