Sugar (and other) transporter;

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2217375326  41 ATFAAVLGNFSFGYALVYTSPVIPALERSLDP-------DLHLTKSQASWFGSVFTLGAAAGGLSAMILNDLLGRKLSIM 113
Cdd:pfam00083   1 VALVAALGGFLFGYDTGVIGAFLTLIDFFKNFglsksvsSLAALSVLSGLIVSIFSVGCFIGSLFAGKLGDRFGRKKSLL 80

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 2217375326 114 FSAVPSAAGYALMAGAHG---LWMLLLGRTLTGFAGGLTAACIPVYVSEIAPPGVRGALGATPQLMAVFGSL 182
Cdd:pfam00083  81 IANVLFVIGAVLQGAAKGkwsVYQLIVGRVLVGIGVGGASVLAPMYISEIAPKKLRGALGSLYQLAITFGIL 152

Predicted arabinose efflux permease AraJ, MFS family [Carbohydrate transport and metabolism];

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2217375326  36 KRVFLATFAAVLGNFSFGYALVYTSPVIPALERsldpDLHLTKSQASWFGSVFTLGAAAGGLSAMILNDLLGRKLSIMFS 115
Cdd:COG2814     6 RRRWLALLALALGAFLSGLGIGIVLPALPLIAA----DLGASPAQAGLVVTAYLLGAALGAPLAGRLADRFGRRRVLLLG 81

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 2217375326 116 AVPSAAGYALMAGAHGLWMLLLGRTLTGFAGGLTAACIPVYVSEIAPPGVRGALGATPQLMAVFGSLSLYALG 188
Cdd:COG2814    82 LLLFALGSLLCALAPSLWLLLAARFLQGLGAGALFPAALALIADLVPPERRGRALGLLGAGLGLGPALGPLLG 154

Glucose transporter (GLUT) types 6 and 8, Class 3 GLUTs, and similar transporters of the Major Facilitator Superfamily; This subfamily is composed of glucose transporter type 6 (GLUT6), GLUT8, plant early dehydration-induced gene ERD6-like proteins, and similar insect proteins including facilitated trehalose transporter Tret1-1. GLUTs, also called Solute carrier family 2, facilitated glucose transporters (SLC2A), are a family of proteins that facilitate the transport of hexoses such as glucose and fructose. There are fourteen GLUTs found in humans; they display different substrate specificities and tissue expression. They have been categorized into three classes based on sequence similarity: Class 1 (GLUTs 1-4, 14); Class 2 (GLUTs 5, 7, 9, and 11); and Class 3 (GLUTs 6, 8, 10, 12, and HMIT). Insect Tret1-1 is a low-capacity facilitative transporter for trehalose that mediates the transport of trehalose synthesized in the fat body and the incorporation of trehalose into other tissues that require a carbon source. GLUT proteins are comprised of about 500 amino acid residues, possess a single N-linked oligosaccharide, and have 12 transmembrane segments. They belong to the Glucose transporter -like (GLUT-like) family of the Major Facilitator Superfamily (MFS) of membrane transport proteins. MFS proteins are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2217375326  39 FLATFAAVLGNFSFGYALVYTSPVIPALERSLDPDLHLTKSQASWFGSVFTLGAAAGGLSAMILNDLLGRKLSIMFSAVP 118
Cdd:cd17358     1 LLATFVANLGSFSFGIAVGWSSPALPSLMSDNSLSLPLTVAQFSWFGSLLTLGALIGALLSGKLADRIGRKRTLLISAIP 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2217375326 119 SAAGYALMAGAHGLWMLLLGRTLTGFAGGLTAACIPVYVSEIAPPGVRGALGATPQLMAVFGSLSLYALGK--PPAAVAL 196
Cdd:cd17358    81 CILGWLLIAFAKDVWMLYLGRFLAGFGGGAASVVVPVYIAEIAPKNVRGALGSLNQLLVNIGILLGYVLGSflPWRTLAL 160

                  ....
gi 2217375326 197 AGCG 200
Cdd:cd17358   161 IGAI 164

Glucose transporters (GLUTs) and other similar sugar transporters of the Major Facilitator Superfamily; This family is composed of glucose transporters (GLUTs) and other sugar transporters including fungal hexose transporters (HXT), bacterial xylose transporter (XylE), plant sugar transport proteins (STP) and polyol transporters (PLT), H(+)-myo-inositol cotransporter (HMIT), and similar proteins. GLUTs, also called Solute carrier family 2, facilitated glucose transporters (SLC2A), are a family of proteins that facilitate the transport of hexoses such as glucose and fructose. There are fourteen GLUTs found in humans; they display different substrate specificities and tissue expression. They have been categorized into three classes based on sequence similarity: Class 1 (GLUTs 1-4, 14); Class 2 (GLUTs 5, 7, 9, and 11); and Class 3 (GLUTs 6, 8, 10, 12, and HMIT). GLUT proteins are comprised of about 500 amino acid residues, possess a single N-linked oligosaccharide, and have 12 transmembrane segments. The GLUT-like family belongs to the Major Facilitator Superfamily (MFS) of membrane transport proteins, which are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2217375326  44 AAVLGNFSFGYALVYTSPVIPALERSLDpdLHLTKSQASWFGSVFTLGAAAGGLSAMILNDLLGRKLSIMFSAVPSAAGY 123
Cdd:cd17315     1 VAALGGLLFGYDLGVINGALLYIAKDLG--FGLSTSLQGLVVSSLLLGAAIGSLFGGPLADRFGRRKSLLIAAVLYVIGA 78

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 2217375326 124 ALMAGAHGLWMLLLGRTLTGFAGGLTAACIPVYVSEIAPPGVRGALGATPQLMAVFGSLSLYALG 188
Cdd:cd17315    79 LLSALAPNVWVLIVGRFLLGLGVGLASVLVPLYISEIAPAKIRGALGTLNQLMITFGILLAYLLG 143

H(+)-myo-inositol cotransporter and similar transporters of the Major Facilitator Superfamily; This subfamily is composed of myo-inositol/inositol transporters and similar transporters from vertebrates, plant, and fungi. The human protein is called H(+)-myo-inositol cotransporter/Proton myo-inositol cotransporter (HMIT), or H(+)-myo-inositol symporter, or Solute carrier family 2 member 13 (SLC2A13). HMIT is classified as a Class 3 GLUT (glucose transporter) based on sequence similarity with GLUTs, but it does not transport glucose. It specifically transports myo-inositol and is expressed predominantly in the brain, with high expression in the hippocampus, hypothalamus, cerebellum and brainstem. HMIT may be involved in regulating processes that require high levels of myo-inositol or its phosphorylated derivatives, such as membrane recycling, growth cone dynamics, and synaptic vesicle exocytosis. Arabidopsis Inositol transporter 4 (AtINT4) mediates high-affinity H+ symport of myo-inositol across the plasma membrane. The HMIT-like subfamily belongs to the Glucose transporter -like (GLUT-like) family of the Major Facilitator Superfamily (MFS) of membrane transport proteins. MFS proteins are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2217375326  44 AAVLGNFSFGYALVYTSPVIPALERSLDPDLHLTKSQASwFGSVFTLGAAAGGLSAMILNDLLGRKLSIMFSAVPSAAGY 123
Cdd:cd17360     1 FAGIGGFLFGYDTGVISGALLYIKDDLGEVVLSTGWQEL-IVSSTVAGAAVGAAIGGWLNDRFGRRPCILLADALFTIGA 79

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 2217375326 124 ALMAGAHGLWMLLLGRTLTGFAGGLTAACIPVYVSEIAPPGVRGALGATPQLMAVFGSLSLYALG 188
Cdd:cd17360    80 IVMAAAPNKEVLIVGRVLVGLGVGIASMTVPLYIAEAAPPRIRGRLVTINVLFITGGQFVAYVIN 144

MFS transporter, sugar porter (SP) family; This model represent the sugar porter subfamily of the major facilitator superfamily (pfam00083) [Transport and binding proteins, Carbohydrates, organic alcohols, and acids]

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2217375326  37 RVFLATFAAVLGNFSFGY--ALVYTSPVIPALERSLDPDLH--LTKSQASWFGSVFTLGAAAGGLSAMILNDLLGRKLSI 112
Cdd:TIGR00879  25 KVALLSLIAAIGGLMFGYdtGVIGGALALPAFEFKFTSANSdsYSSSLWGLVVSIFLVGGFIGALFAGWLSDRFGRKKSL 104

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 2217375326 113 MFSAVPSAAGYALM---AGAHGLWMLLLGRTLTGFAGGLTAACIPVYVSEIAPPGVRGALGATPQLMAVFGSLSLYALG 188
Cdd:TIGR00879 105 LIIALLFVIGAILMglaAFALSVEMLIVGRVLLGIGVGIASALVPMYLSEIAPKALRGALTSLYQLAITFGILVAYGFG 183

Class 1 and Class 2 Glucose transporters (GLUTs) of the Major Facilitator Superfamily; This subfamily includes Class 1 and Class 2 glucose transporters (GLUTs) including Solute carrier family 2, facilitated glucose transporter member 1 (SLC2A1, also called glucose transporter type 1 or GLUT1), SLC2A2-5 (GLUT2-5), SLC2A7 (GLUT7), SLC2A9 (GLUT9), SLC2A11 (GLUT11), SLC2A14 (GLUT14), and similar proteins. GLUTs are a family of proteins that facilitate the transport of hexoses such as glucose and fructose. There are fourteen GLUTs found in humans; they display different substrate specificities and tissue expression. They have been categorized into three classes based on sequence similarity: Class 1 (GLUTs 1-4, 14); Class 2 (GLUTs 5, 7, 9, and 11); and Class 3 (GLUTs 6, 8, 10, 12, and HMIT). GLUTs 1-5 are the most thoroughly studied and are well-established as glucose and/or fructose transporters in various tissues and cell types. GLUT proteins are comprised of about 500 amino acid residues, possess a single N-linked oligosaccharide, and have 12 transmembrane segments. They belong to the Glucose transporter -like (GLUT-like) family of the Major Facilitator Superfamily (MFS) of membrane transport proteins. MFS proteins are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2217375326  41 ATFAAVLGNFSFGYALVYTSPVIPALERSLDPDLH------LTKSQASW----FGSVFTLGAAAGGLSAMILNDLLGRKL 110
Cdd:cd17357     1 AVIVAFGGSFQFGYNIGVVNAPQEVFKEFINDTLVdryglnLSDSELDLlwslIVSIFFIGGAIGSFISAFLANRFGRKN 80

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 2217375326 111 SIMFSAVPSAAGyALMAGAHGLW----MLLLGRTLTGFAGGLTAACIPVYVSEIAPPGVRGALGATPQLMAVFGSL 182
Cdd:cd17357    81 GLLISNALLVVS-SLLMFLSKSAkspeLLIFGRFLVGIACGLSTGFVPMYLQEISPSELRGALGSLTQIGVTLGIL 155

Major Facilitator Superfamily; The Major Facilitator Superfamily (MFS) is a large and diverse group of secondary transporters that includes uniporters, symporters, and antiporters. MFS proteins facilitate the transport across cytoplasmic or internal membranes of a variety of substrates including ions, sugar phosphates, drugs, neurotransmitters, nucleosides, amino acids, and peptides. They do so using the electrochemical potential of the transported substrates. Uniporters transport a single substrate, while symporters and antiporters transport two substrates in the same or in opposite directions, respectively, across membranes. MFS proteins are typically 400 to 600 amino acids in length, and the majority contain 12 transmembrane alpha helices (TMs) connected by hydrophilic loops. The N- and C-terminal halves of these proteins display weak similarity and may be the result of a gene duplication/fusion event. Based on kinetic studies and the structures of a few bacterial superfamily members, GlpT (glycerol-3-phosphate transporter), LacY (lactose permease), and EmrD (multidrug transporter), MFS proteins are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement. Bacterial members function primarily for nutrient uptake, and as drug-efflux pumps to confer antibiotic resistance. Some MFS proteins have medical significance in humans such as the glucose transporter Glut4, which is impaired in type II diabetes, and glucose-6-phosphate transporter (G6PT), which causes glycogen storage disease when mutated.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2217375326  45 AVLGNFSFGYALVYTSPVIPALERsldpDLHLTKSQASWFGSVFTLGAAAGGLSAMILNDLLGRKLSIMFSAVPSAAGYA 124
Cdd:cd06174     1 LLLGFFLTGLARGLISPLLPALLQ----SFGLSASQLGLLFALFSLGYALLQPLAGLLADRFGRRPVLLLGLLLFALGAL 76

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 2217375326 125 LMAGAHGLWMLLLGRTLTGFAGGLTAACIPVYVSEIAPPGVRGALGATPQLMAVFGSLSLYALG 188
Cdd:cd06174    77 LFAFAPSFWLLLLGRFLLGLGSGLIDPAVLALIADLFPERERGRALGLLQAFGSVGGILGPLLG 140

Plant Sugar transport protein subfamily of the Major Facilitator Superfamily of transporters; The plant Sugar transport protein (STP) subfamily includes STP1-STP14; they are also called hexose transporters. They mediate the active uptake of hexoses such as glucose, 3-O-methylglucose, fructose, xylose, mannose, galactose, fucose, 2-deoxyglucose and arabinose, by sugar/hydrogen symport. Several STP family transporters are expressed in a tissue-specific manner, or at specific developmental stages. STP1 is the member with the highest expression level of all members and high expression is detected in photosynthetic tissues, such as leaves and stems, while roots, siliques, and flowers show lower expression levels. It plays a major role in the uptake and response of Arabidopsis seeds and seedlings to sugars. The STP subfamily belongs to the Glucose transporter -like (GLUT-like) family of the Major Facilitator Superfamily (MFS) of membrane transport proteins. MFS proteins are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2217375326  44 AAVLGNFSFGYALVYTSPVI--PALERSLDPDLHLTKSQAS-------------WFGSVFTLGAAAGGLSAMILNDLLGR 108
Cdd:cd17361     1 VAALGGLIFGYDIGISGGVTsmDPFLEKFFPSVYEKKKGASennyckyddqllqLFTSSLYLAGLVASLLASYVTRKWGR 80

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 2217375326 109 KLSIMFSAVPSAAGYALMAGAHGLWMLLLGRTLTGFAGGLTAACIPVYVSEIAPPGVRGALGATPQLMAVFGSL 182
Cdd:cd17361    81 KPSMLIGGVLFLVGAALNAAAQNIAMLIVGRILLGFGVGFGNQAVPLYLSEMAPAKLRGALNIGFQLAITIGIL 154

Purine ribonucleoside efflux pump NepI and similar transporters of the Major Facilitator Superfamily; This family is composed of purine efflux pumps such as Escherichia coli NepI and Bacillus subtilis PbuE, sugar efflux transporters such as Corynebacterium glutamicum arabinose efflux permease, multidrug resistance (MDR) transporters such as Streptomyces lividans chloramphenicol resistance protein (CmlR), and similar proteins. NepI and PbuE are involved in the efflux of purine ribonucleosides such as guanosine, adenosine and inosine, as well as purine bases like guanine, adenine, and hypoxanthine, and purine base analogs. They play a role in the maintenance of cellular purine base pools, as well as in protecting the cells and conferring resistance against toxic purine base analogs such as 6-mercaptopurine. MDR transporters are drug/H+ antiporters (DHA) that mediate the efflux of a variety of drugs and toxic compounds, and confer resistance to these compounds. The NepI-like family belongs to the Major Facilitator Superfamily (MFS) of membrane transport proteins, which are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2217375326  42 TFAAVLGNFSFGYALVYTSPVIPALERsldpDLHLTKSQASWFGSVFTLGAAAGGLSAMILNDLLGRKLSIMFSAVPSAA 121
Cdd:cd17324     1 LLALALAAFAIGTTEYVIQGLLPDIAR----DFGVSVAQAGLLVSAYALGYALGALLLGPLSDRIERRRLLLVLLLLFIL 76

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*.
gi 2217375326 122 GYALMAGAHGLWMLLLGRTLTGFAGGLTAACIPVYVSEIAPPGVRG 167
Cdd:cd17324    77 GNLLAALAPSFALLLLARALAGLAHGGFWAIAAAYAADLVPPEKRG 122

Major Facilitator Superfamily;

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2217375326  38 VFLATFAAVLGNFSFGyalvytspviPALERSLDPDLHLTKSQASWFGSVFTLGAAAGGLSAMILNDLLGRKLSIMFSAV 117
Cdd:pfam07690   1 LFLAAFLAALGRSLLG----------PALPLLLAEDLGISPTEIGLLLTLFSLGYALAQPLAGRLSDRFGRRRVLLIGLL 70

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 2217375326 118 PSAAGYALMAGAHGLWMLLLGRTLTGFAGGLTAACIPVYVSEIAPPGVRGALGATPQLMAVFGSLSLYALG 188
Cdd:pfam07690  71 LFALGLLLLLFASSLWLLLVLRVLQGLGAGALFPAALALIADWFPPEERGRALGLVSAGFGLGAALGPLLG 141

Glucose transporter (GLUT) types 10 and 12, Class 3 GLUTs, and similar transporters of the Major Facilitator Superfamily; This subfamily is composed of glucose transporter type 10, GLUT12, plant polyol transporters (PLTs), and similar proteins. GLUTs, also called Solute carrier family 2, facilitated glucose transporters (SLC2A), are a family of proteins that facilitate the transport of hexoses such as glucose and fructose. There are fourteen GLUTs found in humans; they display different substrate specificities and tissue expression. They have been categorized into three classes based on sequence similarity: Class 1 (GLUTs 1-4, 14); Class 2 (GLUTs 5, 7, 9, and 11); and Class 3 (GLUTs 6, 8, 10, 12, and HMIT). GLUT proteins are comprised of about 500 amino acid residues, possess a single N-linked oligosaccharide, and have 12 transmembrane segments. They belong to the Glucose transporter -like (GLUT-like) family of the Major Facilitator Superfamily (MFS) of membrane transport proteins. MFS proteins are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2217375326  45 AVLGNFSFGYALVYTSPV---IPALERSLDPDLHLTKSQASWFGSVFTLGAAAGGLSAMILNDLLGRKLSIMFSAVPSAA 121
Cdd:cd17362     2 PALGGLLFGYDIGATSGAllsITSPALSGTDWYNLSSLQSGLVVSGSLLGALLGSLVAGALIDRLGRRKELILAALLYLV 81

                          90       100       110       120       130       140
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 2217375326 122 GYALMAGAHGLWMLLLGRTLTGFAGGLTAACIPVYVSEIAPPGVRGALGATPQLMAVFGSLSLYALG 188
Cdd:cd17362    82 GSLVTGLAPSYPVLLVGRLIYGVGIGLAMHAAPVYIAETSPSHIRGLLVSLKELFIVLGILLGYVSG 148

Class 2 Glucose transporters (GLUTs) of the Major Facilitator Superfamily; GLUTs, also called Solute carrier family 2, facilitated glucose transporters (SLC2A), are a family of proteins that facilitate the transport of hexoses such as glucose and fructose. There are fourteen GLUTs found in humans; they display different substrate specificities and tissue expression. They have been categorized into three classes based on sequence similarity: Class 1 (GLUTs 1-4, 14); Class 2 (GLUTs 5, 7, 9, and 11); and Class 3 (GLUTs 6, 8, 10, 12, and HMIT). GLUT5, also called Solute carrier family 2, facilitated glucose transporter member 5 (SLC2A5), is a well-established fructose transporter found in the small intestine. GLUT7 (or SLC2A7) is a high-affinity glucose and fructose transporter expressed in the small intestine and colon. GLUT9 (or SLC2A9) transports urate and fructose, and is most strongly expressed in the basolateral membranes of proximal renal tubular cells, liver and placenta. It may play a role in urate reabsorption by proximal tubules. GLUT11 (or SLC2A11) is a facilitative glucose transporter expressed in heart and skeletal muscle. GLUT proteins are comprised of about 500 amino acid residues, possess a single N-linked oligosaccharide, and have 12 transmembrane segments. They belong to the Glucose transporter -like (GLUT-like) family of the Major Facilitator Superfamily (MFS) of membrane transport proteins. MFS proteins are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2217375326  86 SVFTLGAAAGGLSAMILNDLLGRKLSIMFSAVPSAAGYALMA---GAHGLWMLLLGRTLTGFAGGLTAACIPVYVSEIAP 162
Cdd:cd17432    57 SIFPLGGLFGSLLVGPLVIRLGRKGTLLLNNIFAIVAAILMGlskIAKSFEMIIVGRFLVGINAGISLNVVPMYLGESAP 136

                          90       100
                  ....*....|....*....|....*.
gi 2217375326 163 PGVRGALGATPQLMAVFGSLSLYALG 188
Cdd:cd17432   137 KNLRGAVGLVPAIFITLGILLGQVLG 162

Fucose permease [Carbohydrate transport and metabolism];

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2217375326  34 QNKRVFLATFAAVLGNFSFGYALVYTSPVIPALERsldpDLHLTKSQASWFGSVFTLGAAAGGLSAMILNDLLGRKLSIM 113
Cdd:COG0738     2 SKKNYRRALIAIYLLFFLWGFATALWGPLIPALKD----AFGLSDAQAGLLLFAFFLGYLLASLPAGRLIDRFGYKRGLL 77

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*....
gi 2217375326 114 FSAVPSAAGYALMAGAHGLWMLLLGRTLTGFAGGLTAACIPVYVSEIAP 162
Cdd:COG0738    78 LGLLLMALGLLLFALAPSYPLLLLALFLLGLGLGLLDVAANPYVAALGP 126

Putative arabinose efflux permease family transporters of the Major Facilitator Superfamily; This family includes a group of putative arabinose efflux permease family transporters, such as alpha proteobacterium quinolone resistance protein NorA (characterized Staphylococcus aureus Quinolone resistance protein NorA belongs to a different group), Desulfovibrio dechloracetivorans bacillibactin exporter, Vibrio aerogenes antiseptic resistance protein. The biological function of those transporters remain unclear. They belong to the Major Facilitator Superfamily (MFS) of membrane transport proteins, which are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2217375326  17 PEKPPPSPGDRARVGTLQNKRVFLATFAAVLGNFSFgYALVYTSPVIPALERSLDPdlhltkSQASWFGSVFTLGAAAGG 96
Cdd:cd17473   178 RKSKSAASPGAATAAALPWKRVLPIYFLAFLAMLLF-YVIPIQLPFLLQERGGGSA------ALIGLALAIASLAGAIGA 250

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 2217375326  97 LSAMILNDLLGRKLSIMFSAVPSAAGYALMAGAHGLWMLLLGRTLTGFAGGLTAACIPVYVSEIAPPGVRG 167
Cdd:cd17473   251 LLFGRLKRRLGKRRLLAIGFALMALGFLLLALASGLWVVLLGAILAGLGFGLLLPTLNSWAMSLVPPELRG 321

Metazoan Synaptic vesicle glycoprotein 2 (SV2) and related small molecule transporters of the Major Facilitator Superfamily; This family is composed of metazoan synaptic vesicle glycoprotein 2 (SV2) and related small molecule transporters including those that transport inorganic phosphate (Pht), aromatic compounds (PcaK and related proteins), proline/betaine (ProP), alpha-ketoglutarate (KgtP), citrate (CitA), shikimate (ShiA), and cis,cis-muconate (MucK), among others. SV2 is a transporter-like protein that serves as the receptor for botulinum neurotoxin A (BoNT/A), one of seven neurotoxins produced by the bacterium Clostridium botulinum. BoNT/A blocks neurotransmitter release by cleaving synaptosome-associated protein of 25 kD (SNAP-25) within presynaptic nerve terminals. Also included in this family is synaptic vesicle 2 (SV2)-related protein (SVOP) and similar proteins. SVOP is a transporter-like nucleotide binding protein that localizes to neurotransmitter-containing vesicles. The SV2-like family belongs to the Major Facilitator Superfamily (MFS) of membrane transport proteins, which are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2217375326  30 VGTLQNKRVFLATFAAVLGNFSFgYALVYTSPVIpalersLDPDLHLTKSQASWFGSVFTLGAAAGGLSAMILNDLLGRK 109
Cdd:cd17316   171 LALLLRRRTLLLILLWFFISFGY-YGLTTFLPTY------LQTVLGLSPATSSLYLLLISLGALVGALIAGLLSDRIGRK 243

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2217375326 110 LSIMFSAVPSAAGYAL--MAGAHGLWMLLLGRTLTGFAGGLTAACIPVYVSEIAPPGVRG-ALGATPQLMAVFGSLSLYA 186
Cdd:cd17316   244 KTLVIGLILSGILALPlfYLLSGSPTLLLLLLFILSFFVGGVWGALYAYLAELFPTEVRAtGVGLSYNLGRLGGGGAPPL 323

                  ..
gi 2217375326 187 LG 188
Cdd:cd17316   324 IA 325

Cyanate permease [Inorganic ion transport and metabolism];

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2217375326  36 KRVFLATFAAVLGNFSFGYALVYTSPVIPALERsldpDLHLTKSQASWFGSVFTLGAAAGGLSAMILNDLLGRKLSIMFS 115
Cdd:COG2807    14 AGRWLLLLAIVLVALNLRPAITSVGPLLPEIRA----DLGLSATQAGLLTTLPLLAFGLFAPLAPRLARRFGLERTLLLA 89

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*..
gi 2217375326 116 AVPSAAGYALMAGAHGLWMLLLGRTLTGFAGGLTAACIPVYVSEIAP 162
Cdd:COG2807    90 LLLLAAGLLLRSLAPSVALLLAGTALIGAGIAVGNVLLPGLIKRWFP 136

Sugar phosphate permease [Carbohydrate transport and metabolism];

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2217375326  74 LHLTKSQASWFGSVFTLGAAAGGLSAMILNDLLGR--KLSIMFSAVPSAAGYALMAGAHGLWMLLLGRTLTGFAGGLTAA 151
Cdd:COG2271   211 RGLSLAQAGLLLSLPFLAGIVGSLLGGWLSDRLGRrrKLVLAIGLLLAALALLLLALLPSPALAIALLFLAGFGLGGAFG 290

                          90       100       110
                  ....*....|....*....|....*....|
gi 2217375326 152 CIPVYVSEIAPPGVRGALGATPQLMAVFGS 181
Cdd:COG2271   291 LLWALAAELFPKKARGTASGLVNTFGFLGG 320

bacterial MdtG-like and eukaryotic solute carrier 18 (SLC18) family of the Major Facilitator Superfamily of transporters; This family is composed of eukaryotic solute carrier 18 (SLC18) family transporters and related bacterial multidrug resistance (MDR) transporters including several proteins from Escherichia coli such as multidrug resistance protein MdtG, from Bacillus subtilis such as multidrug resistance proteins 1 (Bmr1) and 2 (Bmr2), and from Staphylococcus aureus such as quinolone resistance protein NorA. The family also includes Escherichia coli arabinose efflux transporters YfcJ and YhhS. MDR transporters are drug/H+ antiporters (DHA) that mediate the efflux of a variety of drugs and toxic compounds, and confer resistance to these compounds. The SLC18 transporter family includes vesicular monoamine transporters (VAT1 and VAT2), vesicular acetylcholine transporter (VAChT), and SLC18B1, which is proposed to be a vesicular polyamine transporter (VPAT). The MdtG/SLC18 family belongs to the Major Facilitator Superfamily (MFS) of membrane transport proteins, which are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2217375326  38 VFLATFAavlgnFSFGYALVytSPVIPALERSLdpdlHLTKSQASWFGSVFTLGAAAGGLSAMILNDLLGRKLSIMFSAV 117
Cdd:cd17325     1 LSLALFL-----DMLGYGII--IPILPLYAESL----GASVAQIGLIVAAYALAQLLFSPPAGRLSDRFGRKRLLLLGLL 69

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|
gi 2217375326 118 PSAAGYALMAGAHGLWMLLLGRTLTGFAGGLTAACIPVYVSEIAPPGVRG 167
Cdd:cd17325    70 LLAVSTLLFAFATSYWQLLLARFLQGLASAAVWPAAMALIADIAPPEERG 119

Plant Nitrate transporter NRT2 family and Bacterial Nitrate/Nitrite transporters of the Major Facilitator Superfamily; This family is composed of plant NRT2 family high-affinity nitrate transporters as well as nitrate and nitrite transporters from bacteria including Bacillus subtilis nitrate transporter NasA and nitrite extrusion protein NarK, Staphylococcus aureus NarT, Synechococcus sp. nitrate permease NapA, Mycobacterium tuberculosis NarK2 and nitrite extrusion protein NarU. NRT2 family proteins are involved in the uptake of nitrate by plant roots from the soil through the high-affinity transport system (HATS). There are seven Arabidopsis thaliana NRT2 proteins, called AtNRT2:1 to AtNRT2:7. The NRT2-like family belongs to the Major Facilitator Superfamily (MFS) of membrane transport proteins, which are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2217375326  38 VFLATFAAVLGnFSFGYALvytSPVIPALERsldpDLHLTKSQASWFGSVFTLGAAAGGLSAMILNDLLG-RKLSIMFSA 116
Cdd:cd17341     1 LHLSWLSFFLG-FMVWFLF---APLGPVIRD----DLGLSTSQIGLLVAIPVLAGALLRLPYGFLCDRFGgRRVFIASLL 72

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|....*
gi 2217375326 117 VPSAAGYALMAGAHGLWMLLLGRTLTGFAGGLTAACIPvYVSEIAPPG-VRGALG 170
Cdd:cd17341    73 LLLIPLLALALAVSSYWGLLVLGFLLGFAGANFASGQP-WASFWFPKEkIGGAAG 126

Glucose transporter type 12 (GLUT12), a Class 3 GLUT, of the Major Facilitator Superfamily of transporters; Glucose transporter type 12 (GLUT12) is also called Solute carrier family 2, facilitated glucose transporter member 12 (SLC2A12). It is a facilitative glucose transporter, classified as a Class 3 GLUT, and is expressed in the heart, skeletal muscle, prostate, and small intestine, and is highly upregulated in breast ductal cell carcinoma. It plays a role as a secondary insulin-sensitive glucose transporter in insulin-dependent tissues. The GLUT12 subfamily belongs to the Glucose transporter -like (GLUT-like) family of the Major Facilitator Superfamily (MFS) of membrane transport proteins. MFS proteins are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2217375326  74 LHLTKSQASWFGSVFTLGAAAGGLSAMILNDLLGRKLSIMFSAVPSAAGYALMAGAHGLWMLLLGRTLTGFAGGLTAACI 153
Cdd:cd17435    31 LALSCHEQEMVVSSLLIGALLASLTGGVLIDRYGRRTAIILTSCLLVLGSLLLVCSVSYTVLIVGRIAIGVSISLSSIAT 110

                          90       100       110
                  ....*....|....*....|....*....|..
gi 2217375326 154 PVYVSEIAPPGVRGALGATPQLMAVFGSLSLY 185
Cdd:cd17435   111 CVYIAEIAPQHRRGLLVSLNELMIVIGILLAY 142

Macrolide efflux protein A and similar proteins of the Major Facilitator Superfamily of transporters; This family is composed of Streptococcus pyogenes macrolide efflux protein A (MefA) and similar transporters, many of which remain uncharacterized. Some members may be multidrug resistance (MDR) transporters, which are drug/H+ antiporters (DHAs) that mediate the efflux of a variety of drugs and toxic compounds, conferring resistance to these compounds. MefA confers resistance to 14-membered macrolides including erythromycin and to 15-membered macrolides. It functions as an efflux pump to regulate intracellular macrolide levels. The MefA-like family belongs to the Major Facilitator Superfamily (MFS) of membrane transport proteins, which are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2217375326  17 PEKPPPSPGDRARVGTLQNKRVFLATFAAVLGNFSFGYALVYTSPVIPALERsldpDLHLTKSQASWFGSVFTLGAAAGG 96
Cdd:cd06173   186 PGESSSLLLRDLREGLRYLRRSPLLRLLLLALALFALLGGALTVLLPLLAKE----VLGGGAAGYGLLLAAFGVGALLGA 261

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2217375326  97 LSAMILNDLLGRKLSIMFSAVPSAAGYALMAGAHGLWMLLLGRTLTGFAGGLTAACIPVYVSEIAPPGVRGalgatpqlm 176
Cdd:cd06173   262 LLLGRLSKRRRRGRLLLIGALLLGLALLVLGLSPSLWLLLAALFLLGLAGGLFNVPLNTLLQLRVPDELRG--------- 332