NCBI Home Page NCBI Site Search page NCBI Guide that lists and describes the NCBI resources
Conserved domains on  [gi|1658212342|ref|XP_029108941|]
View 

Krueppel-like factor 4 isoform X3 [Scleropages formosus]

Protein Classification

Graphical summary

 Zoom to residue level

show extra options »

Show site features     Horizontal zoom: ×

List of domain hits

 Name Accession Description Interval E-value
KLF4_N cd21582
N-terminal domain of Kruppel-like factor 4; Kruppel-like factor 4 (KLF4; also known as ...
 7-333 2.76e-169

N-terminal domain of Kruppel-like factor 4; Kruppel-like factor 4 (KLF4; also known as Krueppel-like factor 4 or gut-enriched Kruppel-like factor/GKLF) is a protein that, in humans, is encoded by the KLF4 gene. Evidence also suggests that KLF4 is a tumor suppressor in certain cancers, including colorectal cancer, gastric cancer, esophageal squamous cell carcinoma, intestinal cancer, prostate cancer, bladder cancer and lung cancer. It may act as a tumor promoter where increased KLF4 expression has been reported, such as in oral squamous cell carcinoma and in primary breast ductal carcinoma. KLF4 is one of four key factors that are essential for inducing pluripotent stem cells. KLF4 is highly expressed in non-dividing cells and its overexpression induces cell cycle arrest. KLF proteins KLF1, KLF2, KLF4, KLF5, KLF6, and KLF7 are transcriptional activators. KLF4 belongs to a family of proteins called the Specificity Protein (SP)/KLF family, characterized by a C-terminal DNA-binding domain of 81 amino acids consisting of three Kruppel-like C2H2 zinc fingers. These factors bind to a loose consensus motif, namely NNRCRCCYY (where N is any nucleotide; R is A/G, and Y is C/T), such as the recurring motifs in GC and GT boxes (5'-GGGGCGGGG-3' and 5-GGTGTGGGG-3') that are present in promoters and more distal regulatory elements of mammalian genes. Members of the KLF family can act as activators or repressors of transcription depending on cell and promoter context. KLFs regulate various cellular functions, such as proliferation, differentiation, and apoptosis, as well as the development and homeostasis of several types of tissue. In addition to the C-terminal DNA-binding domain, each KLF also has a unique N-terminal activation/repression domain that confers specificity and allows it to bind specifically to a certain partner, leading to distinct activities in vivo. This model represents the N-terminal domain of KLF4, which is related to the N-terminal domains of KLF1 and KLF2.


:

Pssm-ID: 409228 [Multi-domain]  Cd Length: 335  Bit Score: 477.26  E-value: 2.76e-169
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1658212342   7 DFDMALSGTLLPSISTFASGPAGvrsKSAGAAAGLAASRWREELSHLKRPCGPSSGGYPDHDIHANLVKKDPEDLDLLDY 86
Cdd:cd21582     1 EFDMALSGTLLPSISTFASGPAV---KEKALGQGSANNRWREELSHLKRPCPPLGGRCYDQDPPLGMRRKEEEFNDLLDY 77

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1658212342  87 DFILSNTMQQ-QQQQQQQDSTVGSCRNLPTSPASYQLPSPQGTASDVLYTIP-----DVNDVPPSGGFVAELMRPELDPA 160
Cdd:cd21582    78 DFILSNSLLQqQQQQQQAASTSSSTSSSSSSPYSYQLPSPQGTATSFLYPIPranlaDINDVSPSGGFVAELMRPDLDPA 157

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1658212342 161 YLQPTSLHGKFVVKTTMDMAEYSQSINVGK------SSTSLEPSVSFVCPRIKQENPSTCTISQPMDTHHGVNSHPGTCQ 234
Cdd:cd21582   158 YLQPTSLHGKFVVKTTMDMGDYSQSINVSKsapmtkSSVAPSSSLPFMCPRIKQENPSTCTISRPMDGHLGGNSQHGFSQ 237

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1658212342 235 RPLLGPHDFTSGRPRVASQTSASlpqEVMSRDHLPTAQVLSHPPLPLSQGYHISAGYPPFQAPPSL--QYQELLSPGDSL 312
Cdd:cd21582   238 RAPLPSRTTPSGGPGGGNSSTAE---SLMSRDHHPSSQVLSHPPLPLPQGYHPSPGYPPFPPPPSQpqQYQELMSPGSCL 314

                         330       340
                  ....*....|....*....|.
gi 1658212342 313 PEEPKPKRGRRSWPRKRIATH 333
Cdd:cd21582   315 PEEPKPKRGRRSWPRKRTATH 335
zf-H2C2_2 pfam13465
Zinc-finger double domain;
379-404 4.02e-05

Zinc-finger double domain;


:

Pssm-ID: 463886 [Multi-domain]  Cd Length: 26  Bit Score: 40.05  E-value: 4.02e-05
                          10        20
                  ....*....|....*....|....*.
gi 1658212342 379 ELTRHYRKHTGHRPFQCQKCDRAFSR 404
Cdd:pfam13465   1 NLKRHMRTHTGEKPYKCPECGKSFKS 26
zf-C2H2 pfam00096
Zinc finger, C2H2 type; The C2H2 zinc finger is the classical zinc finger domain. The two ...
 333-357 3.16e-03

Zinc finger, C2H2 type; The C2H2 zinc finger is the classical zinc finger domain. The two conserved cysteines and histidines co-ordinate a zinc ion. The following pattern describes the zinc finger. #-X-C-X(1-5)-C-X3-#-X5-#-X2-H-X(3-6)-[H/C] Where X can be any amino acid, and numbers in brackets indicate the number of residues. The positions marked # are those that are important for the stable fold of the zinc finger. The final position can be either his or cys. The C2H2 zinc finger is composed of two short beta strands followed by an alpha helix. The amino terminal part of the helix binds the major groove in DNA binding zinc fingers. The accepted consensus binding sequence for Sp1 is usually defined by the asymmetric hexanucleotide core GGGCGG but this sequence does not include, among others, the GAG (=CTC) repeat that constitutes a high-affinity site for Sp1 binding to the wt1 promoter.


:

Pssm-ID: 395048 [Multi-domain]  Cd Length: 23  Bit Score: 34.97  E-value: 3.16e-03
                          10        20
                  ....*....|....*....|....*
gi 1658212342 333 HTCDYagCGKTYTKSSHLKAHHRTH 357
Cdd:pfam00096   1 YKCPD--CGKSFSRKSNLKRHLRTH 23
 
Name Accession Description Interval E-value
KLF4_N cd21582
N-terminal domain of Kruppel-like factor 4; Kruppel-like factor 4 (KLF4; also known as ...
 7-333 2.76e-169

N-terminal domain of Kruppel-like factor 4; Kruppel-like factor 4 (KLF4; also known as Krueppel-like factor 4 or gut-enriched Kruppel-like factor/GKLF) is a protein that, in humans, is encoded by the KLF4 gene. Evidence also suggests that KLF4 is a tumor suppressor in certain cancers, including colorectal cancer, gastric cancer, esophageal squamous cell carcinoma, intestinal cancer, prostate cancer, bladder cancer and lung cancer. It may act as a tumor promoter where increased KLF4 expression has been reported, such as in oral squamous cell carcinoma and in primary breast ductal carcinoma. KLF4 is one of four key factors that are essential for inducing pluripotent stem cells. KLF4 is highly expressed in non-dividing cells and its overexpression induces cell cycle arrest. KLF proteins KLF1, KLF2, KLF4, KLF5, KLF6, and KLF7 are transcriptional activators. KLF4 belongs to a family of proteins called the Specificity Protein (SP)/KLF family, characterized by a C-terminal DNA-binding domain of 81 amino acids consisting of three Kruppel-like C2H2 zinc fingers. These factors bind to a loose consensus motif, namely NNRCRCCYY (where N is any nucleotide; R is A/G, and Y is C/T), such as the recurring motifs in GC and GT boxes (5'-GGGGCGGGG-3' and 5-GGTGTGGGG-3') that are present in promoters and more distal regulatory elements of mammalian genes. Members of the KLF family can act as activators or repressors of transcription depending on cell and promoter context. KLFs regulate various cellular functions, such as proliferation, differentiation, and apoptosis, as well as the development and homeostasis of several types of tissue. In addition to the C-terminal DNA-binding domain, each KLF also has a unique N-terminal activation/repression domain that confers specificity and allows it to bind specifically to a certain partner, leading to distinct activities in vivo. This model represents the N-terminal domain of KLF4, which is related to the N-terminal domains of KLF1 and KLF2.


Pssm-ID: 409228 [Multi-domain]  Cd Length: 335  Bit Score: 477.26  E-value: 2.76e-169
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1658212342   7 DFDMALSGTLLPSISTFASGPAGvrsKSAGAAAGLAASRWREELSHLKRPCGPSSGGYPDHDIHANLVKKDPEDLDLLDY 86
Cdd:cd21582     1 EFDMALSGTLLPSISTFASGPAV---KEKALGQGSANNRWREELSHLKRPCPPLGGRCYDQDPPLGMRRKEEEFNDLLDY 77

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1658212342  87 DFILSNTMQQ-QQQQQQQDSTVGSCRNLPTSPASYQLPSPQGTASDVLYTIP-----DVNDVPPSGGFVAELMRPELDPA 160
Cdd:cd21582    78 DFILSNSLLQqQQQQQQAASTSSSTSSSSSSPYSYQLPSPQGTATSFLYPIPranlaDINDVSPSGGFVAELMRPDLDPA 157

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1658212342 161 YLQPTSLHGKFVVKTTMDMAEYSQSINVGK------SSTSLEPSVSFVCPRIKQENPSTCTISQPMDTHHGVNSHPGTCQ 234
Cdd:cd21582   158 YLQPTSLHGKFVVKTTMDMGDYSQSINVSKsapmtkSSVAPSSSLPFMCPRIKQENPSTCTISRPMDGHLGGNSQHGFSQ 237

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1658212342 235 RPLLGPHDFTSGRPRVASQTSASlpqEVMSRDHLPTAQVLSHPPLPLSQGYHISAGYPPFQAPPSL--QYQELLSPGDSL 312
Cdd:cd21582   238 RAPLPSRTTPSGGPGGGNSSTAE---SLMSRDHHPSSQVLSHPPLPLPQGYHPSPGYPPFPPPPSQpqQYQELMSPGSCL 314

                         330       340
                  ....*....|....*....|.
gi 1658212342 313 PEEPKPKRGRRSWPRKRIATH 333
Cdd:cd21582   315 PEEPKPKRGRRSWPRKRTATH 335
zf-H2C2_2 pfam13465
Zinc-finger double domain;
379-404 4.02e-05

Zinc-finger double domain;


Pssm-ID: 463886 [Multi-domain]  Cd Length: 26  Bit Score: 40.05  E-value: 4.02e-05
                          10        20
                  ....*....|....*....|....*.
gi 1658212342 379 ELTRHYRKHTGHRPFQCQKCDRAFSR 404
Cdd:pfam13465   1 NLKRHMRTHTGEKPYKCPECGKSFKS 26
zf-C2H2 pfam00096
Zinc finger, C2H2 type; The C2H2 zinc finger is the classical zinc finger domain. The two ...
 333-357 3.16e-03

Zinc finger, C2H2 type; The C2H2 zinc finger is the classical zinc finger domain. The two conserved cysteines and histidines co-ordinate a zinc ion. The following pattern describes the zinc finger. #-X-C-X(1-5)-C-X3-#-X5-#-X2-H-X(3-6)-[H/C] Where X can be any amino acid, and numbers in brackets indicate the number of residues. The positions marked # are those that are important for the stable fold of the zinc finger. The final position can be either his or cys. The C2H2 zinc finger is composed of two short beta strands followed by an alpha helix. The amino terminal part of the helix binds the major groove in DNA binding zinc fingers. The accepted consensus binding sequence for Sp1 is usually defined by the asymmetric hexanucleotide core GGGCGG but this sequence does not include, among others, the GAG (=CTC) repeat that constitutes a high-affinity site for Sp1 binding to the wt1 promoter.


Pssm-ID: 395048 [Multi-domain]  Cd Length: 23  Bit Score: 34.97  E-value: 3.16e-03
                          10        20
                  ....*....|....*....|....*
gi 1658212342 333 HTCDYagCGKTYTKSSHLKAHHRTH 357
Cdd:pfam00096   1 YKCPD--CGKSFSRKSNLKRHLRTH 23
 
Name Accession Description Interval E-value
KLF4_N cd21582
N-terminal domain of Kruppel-like factor 4; Kruppel-like factor 4 (KLF4; also known as ...
 7-333 2.76e-169

N-terminal domain of Kruppel-like factor 4; Kruppel-like factor 4 (KLF4; also known as Krueppel-like factor 4 or gut-enriched Kruppel-like factor/GKLF) is a protein that, in humans, is encoded by the KLF4 gene. Evidence also suggests that KLF4 is a tumor suppressor in certain cancers, including colorectal cancer, gastric cancer, esophageal squamous cell carcinoma, intestinal cancer, prostate cancer, bladder cancer and lung cancer. It may act as a tumor promoter where increased KLF4 expression has been reported, such as in oral squamous cell carcinoma and in primary breast ductal carcinoma. KLF4 is one of four key factors that are essential for inducing pluripotent stem cells. KLF4 is highly expressed in non-dividing cells and its overexpression induces cell cycle arrest. KLF proteins KLF1, KLF2, KLF4, KLF5, KLF6, and KLF7 are transcriptional activators. KLF4 belongs to a family of proteins called the Specificity Protein (SP)/KLF family, characterized by a C-terminal DNA-binding domain of 81 amino acids consisting of three Kruppel-like C2H2 zinc fingers. These factors bind to a loose consensus motif, namely NNRCRCCYY (where N is any nucleotide; R is A/G, and Y is C/T), such as the recurring motifs in GC and GT boxes (5'-GGGGCGGGG-3' and 5-GGTGTGGGG-3') that are present in promoters and more distal regulatory elements of mammalian genes. Members of the KLF family can act as activators or repressors of transcription depending on cell and promoter context. KLFs regulate various cellular functions, such as proliferation, differentiation, and apoptosis, as well as the development and homeostasis of several types of tissue. In addition to the C-terminal DNA-binding domain, each KLF also has a unique N-terminal activation/repression domain that confers specificity and allows it to bind specifically to a certain partner, leading to distinct activities in vivo. This model represents the N-terminal domain of KLF4, which is related to the N-terminal domains of KLF1 and KLF2.


Pssm-ID: 409228 [Multi-domain]  Cd Length: 335  Bit Score: 477.26  E-value: 2.76e-169
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1658212342   7 DFDMALSGTLLPSISTFASGPAGvrsKSAGAAAGLAASRWREELSHLKRPCGPSSGGYPDHDIHANLVKKDPEDLDLLDY 86
Cdd:cd21582     1 EFDMALSGTLLPSISTFASGPAV---KEKALGQGSANNRWREELSHLKRPCPPLGGRCYDQDPPLGMRRKEEEFNDLLDY 77

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1658212342  87 DFILSNTMQQ-QQQQQQQDSTVGSCRNLPTSPASYQLPSPQGTASDVLYTIP-----DVNDVPPSGGFVAELMRPELDPA 160
Cdd:cd21582    78 DFILSNSLLQqQQQQQQAASTSSSTSSSSSSPYSYQLPSPQGTATSFLYPIPranlaDINDVSPSGGFVAELMRPDLDPA 157

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1658212342 161 YLQPTSLHGKFVVKTTMDMAEYSQSINVGK------SSTSLEPSVSFVCPRIKQENPSTCTISQPMDTHHGVNSHPGTCQ 234
Cdd:cd21582   158 YLQPTSLHGKFVVKTTMDMGDYSQSINVSKsapmtkSSVAPSSSLPFMCPRIKQENPSTCTISRPMDGHLGGNSQHGFSQ 237

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1658212342 235 RPLLGPHDFTSGRPRVASQTSASlpqEVMSRDHLPTAQVLSHPPLPLSQGYHISAGYPPFQAPPSL--QYQELLSPGDSL 312
Cdd:cd21582   238 RAPLPSRTTPSGGPGGGNSSTAE---SLMSRDHHPSSQVLSHPPLPLPQGYHPSPGYPPFPPPPSQpqQYQELMSPGSCL 314

                         330       340
                  ....*....|....*....|.
gi 1658212342 313 PEEPKPKRGRRSWPRKRIATH 333
Cdd:cd21582   315 PEEPKPKRGRRSWPRKRTATH 335
KLF2_N cd21583
N-terminal domain of Kruppel-like factor 2; Kruppel-like Factor 2 (KLF2, also known as ...
 13-333 2.65e-33

N-terminal domain of Kruppel-like factor 2; Kruppel-like Factor 2 (KLF2, also known as Krueppel-like factor 2 or lung Kruppel-like Factor/LKLF) is a protein that, in humans, is encoded by the KLF2 gene on chromosome 19. It has been implicated in a variety of biochemical processes in the human body, including lung development, embryonic erythropoiesis, epithelial integrity, T-cell viability, and adipogenesis. KLF proteins KLF1, KLF2, KLF4, KLF5, KLF6, and KLF7 are transcriptional activators. KLF2 belongs to a family of proteins called the Specificity Protein (SP)/KLF family, characterized by a C-terminal DNA-binding domain of 81 amino acids consisting of three Kruppel-like C2H2 zinc fingers. These factors bind to a loose consensus motif, namely NNRCRCCYY (where N is any nucleotide; R is A/G, and Y is C/T), such as the recurring motifs in GC and GT boxes (5'-GGGGCGGGG-3' and 5-GGTGTGGGG-3') that are present in promoters and more distal regulatory elements of mammalian genes. Members of the KLF family can act as activators or repressors of transcription depending on cell and promoter context. KLFs regulate various cellular functions, such as proliferation, differentiation, and apoptosis, as well as the development and homeostasis of several types of tissue. In addition to the C-terminal DNA-binding domain, each KLF also has a unique N-terminal activation/repression domain that confers specificity and allows it to bind specifically to a certain partner, leading to distinct activities in vivo. This model represents the N-terminal domain of KLF2, which is related to the N-terminal domains of KLF1 and KLF4.


Pssm-ID: 409229 [Multi-domain]  Cd Length: 299  Bit Score: 126.69  E-value: 2.65e-33
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1658212342  13 SGTLLPSISTFASGpagvRSKSAGaaaglaaSRWREELSHlkrpCGPSSGGYPDHDIHANLVKKDPEDLDL-LDYDFILS 91
Cdd:cd21583     1 SGTILPSISTFANQ----KEKCWE-------DRWKGEEDK----SSLSSCLLDLQNGDSCLGRKDDEDLDNyLDLDFILA 65

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1658212342  92 NTMQQQQQQQQQDSTVGSCrnlPTSPASYQLPSPQGTasdvlYTIPDVNDVPP-SGGFVAELMRPELDPAYLQPTSLHGK 170
Cdd:cd21583    66 NTAGSDSAGAAGGGYYGSL---PEPAAPYSASNPPGA-----YSVPEQGSPPPpYSSSLMAELLRDSDSDYGPSNSIQGR 137

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1658212342 171 FVVkTTMDMAEYSQ--SINVGKSSTSLEPSVSFV---CPRIKQENPSTCTISQPMDTHhGVNSHPGTCQRPLLGPHDfts 245
Cdd:cd21583   138 FLV-SSAFFPRQDDpdSIKVEPSMDSYGPVMGMVpqsCPKIKQEGNVSCMMSFEQPRL-ANSPQAAGSMTPPLSPDD--- 212

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1658212342 246 grprvasQTSASLPQEVMSRDHLPTAQVLSHPPLPLSQGYHISAG-YPPFQAPPSLQYQE---LLSPGDSLPE--EPKPK 319
Cdd:cd21583   213 -------LMASECQQQMMCSPSFPQPHHPHYPPPPQPPLQYQSPHqFGLFEDGLPLQPSAqrvLLTPPSSPLEllESKPK 285

                         330
                  ....*....|....
gi 1658212342 320 RGRRSWPRKRIATH 333
Cdd:cd21583   286 RGRRSWPRKRTATH 299
KLF1_2_4_N-like cd22056
N-terminal domain of Kruppel-like factors with similarity to the N-terminal domains of ...
 16-333 2.27e-13

N-terminal domain of Kruppel-like factors with similarity to the N-terminal domains of Kruppel-like factor (KLF)1, KLF2, and KLF4; Kruppel/Krueppel-like transcription factors (KLFs) belong to a family of proteins called the Specificity Protein (SP)/KLF family, characterized by a C-terminal DNA-binding domain of 81 amino acids consisting of three Kruppel-like C2H2 zinc fingers. These factors bind to a loose consensus motif, namely NNRCRCCYY (where N is any nucleotide; R is A/G, and Y is C/T), such as the recurring motifs in GC and GT boxes (5'-GGGGCGGGG-3' and 5-GGTGTGGGG-3') that are present in promoters and more distal regulatory elements of mammalian genes. Members of the KLF family can act as activators or repressors of transcription depending on cell and promoter context. KLFs regulate various cellular functions, such as proliferation, differentiation, and apoptosis, as well as the development and homeostasis of several types of tissue. In addition to the C-terminal DNA-binding domain, each KLF also has a unique N-terminal activation/repression domain that confers specifity and allows it to bind specifically to a certain partner, leading to distinct activities in vivo. This model represents the N-terminal domains of an unknown subfamily of KLFs, predominantly found in fish, related to the N-terminal domains of KLF1, KLF2, and KLF4.


Pssm-ID: 409231 [Multi-domain]  Cd Length: 339  Bit Score: 70.84  E-value: 2.27e-13
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1658212342  16 LLPSISTFASGPAGVRSKSAGAAAGLAASRWRE-ELSHLKRPCGPssggyPDHDIHANLVKKDPEDL-DLLDYDFILSNT 93
Cdd:cd22056     2 MLPSISTFASLQPLEEKQPEILCRWSVDAASSEvEFSIVQSPIEV-----PKDDDELGKFLDEEDILsNFLDLEFILSSS 76

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1658212342  94 MQqqqqqqqqdstvGSCRNLPTSPASYQLP-SPQ--GTASDvlytiPDVNDVP-------------PSGGFVAELMRPEL 157
Cdd:cd22056    77 NG------------SSSGPSQQQQCAYPLPeSPEscSTGSD-----SDGSDAPqpyhgsagftsssPVHSLMAELLTPEM 139

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1658212342 158 DPAYLQPTSLHGKFVVKttmDMAEYSQSINVGKSSTSLEPSVSFVCPRIKQENPS-TCtisqpMDTHHGVNSHPGTCQRP 236
Cdd:cd22056   140 NYPGESSPDSAGKARDG---REYTELRALPTAPPAHQPATSPPPLGYKIKTEPVEqSC-----MMAAGGGGFMGQQKPKH 211

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1658212342 237 LLGPHDFTSGRPRVASQTSASLPQEVMSRDHLPTAQVLSHPPLPLSQGYH-ISAGYPPFQAPPSL-QYQ----------- 303
Cdd:cd22056   212 QMHSVHPQAFTHHQAAGPGALQGRGGRGGPDCHLLHSSHHHHHHHHLQYQyMNAPYPPHYAHQGApQFHgqysvfrepmr 291

                         330       340       350       360       370
                  ....*....|....*....|....*....|....*....|....*....|
gi 1658212342 304 --------------------ELLSPGDslPEEPKPKRGRRSWPRKRIATH 333
Cdd:cd22056   292 vhhqghpgsmltppssppllEFYAQDE--PEDIKPKRGRRSWARKRTATH 339
KLF1_2_4_N cd21972
N-terminal domain of Kruppel-like factor (KLF) 1, KLF2, KLF4, and similar proteins; Kruppel ...
 114-333 1.32e-09

N-terminal domain of Kruppel-like factor (KLF) 1, KLF2, KLF4, and similar proteins; Kruppel/Krueppel-like transcription factors (KLFs) belong to a family of proteins called the Specificity Protein (SP)/KLF family, characterized by a C-terminal DNA-binding domain of 81 amino acids consisting of three Kruppel-like C2H2 zinc fingers. These factors bind to a loose consensus motif, namely NNRCRCCYY (where N is any nucleotide; R is A/G, and Y is C/T), such as the recurring motifs in GC and GT boxes (5'-GGGGCGGGG-3' and 5-GGTGTGGGG-3') that are present in promoters and more distal regulatory elements of mammalian genes. Members of the KLF family can act as activators or repressors of transcription depending on cell and promoter context. KLFs regulate various cellular functions, such as proliferation, differentiation, and apoptosis, as well as the development and homeostasis of several types of tissue. In addition to the C-terminal DNA-binding domain, each KLF also has a unique N-terminal activation/repression domain that confers specifity and allows it to bind specifically to a certain partner, leading to distinct activities in vivo. This model represents the related N-terminal domains of KLF1, KLF2, KLF4, and similar proteins.


Pssm-ID: 409230 [Multi-domain]  Cd Length: 194  Bit Score: 57.30  E-value: 1.32e-09
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1658212342 114 PTSPASYQLPSPQgtasDVLYTIPDVNDVPPSGGFVAELMRPELDPAYLQPTSLHGKFVVKTTMDM----AEYSQSINVG 189
Cdd:cd21972     3 SSLVSSGPFAKPE----DDLSKFLDLEFILSNTVTSDNDNPPPPDPAYPPPESPESCSTVYDSDGChptpNAYCGPNGPG 78

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1658212342 190 KSSTSLEPSVS-FVCPRIKQENPSTCTisqpmdthHGVNSHPGtcqrpllgpHDFTSGRPRVasqtsaslpqevMSRDHL 268
Cdd:cd21972    79 LPGHFLLAGNSpNLGPKIKTENQEQAC--------MPVAGYSG---------HYGPREPQRV------------PPAPPP 129

                         170       180       190       200       210       220
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 1658212342 269 PTAQVLSHPPLPLSQGYHISAGYPPFQAPPSLQYQELLSPGDSLPEEPKPKRGRRSWPRKRIATH 333
Cdd:cd21972   130 PQYAGHFQYHGHFNMFSPPLRANHPGMSTVMLTPLSTPPLGFLSPEEAKPKRGRRSWARKRTATH 194
zf-H2C2_2 pfam13465
Zinc-finger double domain;
379-404 4.02e-05

Zinc-finger double domain;


Pssm-ID: 463886 [Multi-domain]  Cd Length: 26  Bit Score: 40.05  E-value: 4.02e-05
                          10        20
                  ....*....|....*....|....*.
gi 1658212342 379 ELTRHYRKHTGHRPFQCQKCDRAFSR 404
Cdd:pfam13465   1 NLKRHMRTHTGEKPYKCPECGKSFKS 26
zf-C2H2 pfam00096
Zinc finger, C2H2 type; The C2H2 zinc finger is the classical zinc finger domain. The two ...
 393-415 1.56e-04

Zinc finger, C2H2 type; The C2H2 zinc finger is the classical zinc finger domain. The two conserved cysteines and histidines co-ordinate a zinc ion. The following pattern describes the zinc finger. #-X-C-X(1-5)-C-X3-#-X5-#-X2-H-X(3-6)-[H/C] Where X can be any amino acid, and numbers in brackets indicate the number of residues. The positions marked # are those that are important for the stable fold of the zinc finger. The final position can be either his or cys. The C2H2 zinc finger is composed of two short beta strands followed by an alpha helix. The amino terminal part of the helix binds the major groove in DNA binding zinc fingers. The accepted consensus binding sequence for Sp1 is usually defined by the asymmetric hexanucleotide core GGGCGG but this sequence does not include, among others, the GAG (=CTC) repeat that constitutes a high-affinity site for Sp1 binding to the wt1 promoter.


Pssm-ID: 395048 [Multi-domain]  Cd Length: 23  Bit Score: 38.44  E-value: 1.56e-04
                          10        20
                  ....*....|....*....|...
gi 1658212342 393 FQCQKCDRAFSRSDHLALHMKRH 415
Cdd:pfam00096   1 YKCPDCGKSFSRKSNLKRHLRTH 23
KLF1_N cd21581
N-terminal domain of Kruppel-like Factor 1; Kruppel-like Factor 1 (KLF1, also known as ...
 283-333 1.87e-03

N-terminal domain of Kruppel-like Factor 1; Kruppel-like Factor 1 (KLF1, also known as Krueppel-like factor 1 or Erythroid Kruppel-like Factor/EKLF) was the first Kruppel-like factor discovered. It was found to be vitally important for embryonic erythropoiesis in promoting the switch from fetal hemoglobin (Hemoglobin F) to adult hemoglobin (Hemoglobin A) gene expression by binding to highly conserved CACCC domains. EKLF ablation in mouse embryos produces a lethal anemic phenotype, causing death by embryonic day 14, and natural mutations lead to beta+ thalassemia in humans. However, expression of embryonic hemoglobin and fetal hemoglobin genes is normal in EKLF-deficient mice, suggesting other factors may be involved. KLF1 functions as a transcriptional activator. It belongs to a family of proteins, called the Specificity Protein (SP)/KLF family, characterized by a C-terminal DNA-binding domain of 81 amino acids consisting of three Kruppel-like C2H2 zinc fingers. These factors bind to a loose consensus motif, namely NNRCRCCYY (where N is any nucleotide; R is A/G, and Y is C/T), such as the recurring motifs in GC and GT boxes (5'-GGGGCGGGG-3' and 5-GGTGTGGGG-3') that are present in promoters and more distal regulatory elements of mammalian genes. Members of the KLF family can act as activators or repressors of transcription depending on cell and promoter context. KLFs regulate various cellular functions, such as proliferation, differentiation, and apoptosis, as well as the development and homeostasis of several types of tissue. In addition to the C-terminal DNA-binding domain, each KLF also has a unique N-terminal activation/repression domain that confers specifity and allows it to bind specifically to a certain partner, leading to distinct activities in vivo. This model represents the N-terminal domain of KLF1, which is related to the N-terminal domains of KLF2 and KLF4.


Pssm-ID: 409227 [Multi-domain]  Cd Length: 278  Bit Score: 40.03  E-value: 1.87e-03
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....*..
gi 1658212342 283 QGYHISAGYPPFQAPPS-----LQYQELLSPGDSLPEE-PKPKRGRRSWPRKRIATH 333
Cdd:cd21581   222 QLFRHNAALFPDYAHSPgpghlPLGQQPLLPDPPLPPGgAEGKRGRRSWAKKRPAVH 278
zf-C2H2 pfam00096
Zinc finger, C2H2 type; The C2H2 zinc finger is the classical zinc finger domain. The two ...
 333-357 3.16e-03

Zinc finger, C2H2 type; The C2H2 zinc finger is the classical zinc finger domain. The two conserved cysteines and histidines co-ordinate a zinc ion. The following pattern describes the zinc finger. #-X-C-X(1-5)-C-X3-#-X5-#-X2-H-X(3-6)-[H/C] Where X can be any amino acid, and numbers in brackets indicate the number of residues. The positions marked # are those that are important for the stable fold of the zinc finger. The final position can be either his or cys. The C2H2 zinc finger is composed of two short beta strands followed by an alpha helix. The amino terminal part of the helix binds the major groove in DNA binding zinc fingers. The accepted consensus binding sequence for Sp1 is usually defined by the asymmetric hexanucleotide core GGGCGG but this sequence does not include, among others, the GAG (=CTC) repeat that constitutes a high-affinity site for Sp1 binding to the wt1 promoter.


Pssm-ID: 395048 [Multi-domain]  Cd Length: 23  Bit Score: 34.97  E-value: 3.16e-03
                          10        20
                  ....*....|....*....|....*
gi 1658212342 333 HTCDYagCGKTYTKSSHLKAHHRTH 357
Cdd:pfam00096   1 YKCPD--CGKSFSRKSNLKRHLRTH 23
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
Help | Disclaimer | Write to the Help Desk
NCBI | NLM | NIH