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Conserved domains on  [gi|1622949777|ref|XP_028706035|]
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DDB1- and CUL4-associated factor 4 isoform X2 [Macaca mulatta]

Protein Classification

WD40 repeat domain-containing protein( domain architecture ID 1000017)

WD40 repeat domain-containing protein folds into a beta-propeller structure and functions as a scaffold, providing a platform for the interaction and assembly of several proteins into a signalosome; similar to a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly

CATH:  2.130.10.10
Gene Ontology:  GO:0005515
PubMed:  10322433|8090199|30069656|29026209
SCOP:  4002744

Graphical summary

 Zoom to residue level

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List of domain hits

 Name Accession Description Interval E-value
WD40 super family cl29593
WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions ...
 155-255 2.56e-11

WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from its N-terminus and the WD dipeptide at its C-terminus and is 40 residues long, hence the name WD40; between GH and WD lies a conserved core; serves as a stable propeller-like platform to which proteins can bind either stably or reversibly; forms a propeller-like structure with several blades where each blade is composed of a four-stranded anti-parallel b-sheet; instances with few detectable copies are hypothesized to form larger structures by dimerization; each WD40 sequence repeat forms the first three strands of one blade and the last strand in the next blade; the last C-terminal WD40 repeat completes the blade structure of the first WD40 repeat to create the closed ring propeller-structure; residues on the top and bottom surface of the propeller are proposed to coordinate interactions with other proteins and/or small ligands; 7 copies of the repeat are present in this alignment.


The actual alignment was detected with superfamily member cd00200:

Pssm-ID: 475233 [Multi-domain]  Cd Length: 289  Bit Score: 62.74  E-value: 2.56e-11
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1622949777 155 HDSAVTSVRILQDEQYLMASDMAGKIKLWDLRTTKCIRQYEGH---VNEyaylpLHVHEEEGILVAVGQDCYTRIWSLHD 231
Cdd:cd00200    92 HTSYVSSVAFSPDGRILSSSSRDKTIKVWDVETGKCLTTLRGHtdwVNS-----VAFSPDGTFVASSSQDGTIKLWDLRT 166

                          90       100
                  ....*....|....*....|....
gi 1622949777 232 AHLLRTIpspyPASKADIPSVAFS 255
Cdd:cd00200   167 GKCVATL----TGHTGEVNSVAFS 186
 
Name Accession Description Interval E-value
WD40 cd00200
WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions ...
 155-255 2.56e-11

WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from its N-terminus and the WD dipeptide at its C-terminus and is 40 residues long, hence the name WD40; between GH and WD lies a conserved core; serves as a stable propeller-like platform to which proteins can bind either stably or reversibly; forms a propeller-like structure with several blades where each blade is composed of a four-stranded anti-parallel b-sheet; instances with few detectable copies are hypothesized to form larger structures by dimerization; each WD40 sequence repeat forms the first three strands of one blade and the last strand in the next blade; the last C-terminal WD40 repeat completes the blade structure of the first WD40 repeat to create the closed ring propeller-structure; residues on the top and bottom surface of the propeller are proposed to coordinate interactions with other proteins and/or small ligands; 7 copies of the repeat are present in this alignment.


Pssm-ID: 238121 [Multi-domain]  Cd Length: 289  Bit Score: 62.74  E-value: 2.56e-11
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1622949777 155 HDSAVTSVRILQDEQYLMASDMAGKIKLWDLRTTKCIRQYEGH---VNEyaylpLHVHEEEGILVAVGQDCYTRIWSLHD 231
Cdd:cd00200    92 HTSYVSSVAFSPDGRILSSSSRDKTIKVWDVETGKCLTTLRGHtdwVNS-----VAFSPDGTFVASSSQDGTIKLWDLRT 166

                          90       100
                  ....*....|....*....|....
gi 1622949777 232 AHLLRTIpspyPASKADIPSVAFS 255
Cdd:cd00200   167 GKCVATL----TGHTGEVNSVAFS 186
WD40 COG2319
WD40 repeat [General function prediction only];
71-255 1.27e-10

WD40 repeat [General function prediction only];


Pssm-ID: 441893 [Multi-domain]  Cd Length: 403  Bit Score: 61.08  E-value: 1.27e-10
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1622949777  71 AWSCAWSLNIQANNCFSTGLSRRVLLTNVVTGHRQSFGTNSDVLAQQFALMVPLLFNGCRSGEIFAIDLrcgnQGKGWKA 150
Cdd:COG2319    39 VASLAASPDGARLAAGAGDLTLLLLDAAAGALLATLLGHTAAVLSVAFSPDGRLLASASADGTVRLWDL----ATGLLLR 114

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1622949777 151 TRLFHDSAVTSVRILQDEQYLMASDMAGKIKLWDLRTTKCIRQYEGH---VNEYAYLPlhvheeEG-ILVAVGQDCYTRI 226
Cdd:COG2319   115 TLTGHTGAVRSVAFSPDGKTLASGSADGTVRLWDLATGKLLRTLTGHsgaVTSVAFSP------DGkLLASGSDDGTVRL 188

                         170       180
                  ....*....|....*....|....*....
gi 1622949777 227 WSLHDAHLLRTIpspyPASKADIPSVAFS 255
Cdd:COG2319   189 WDLATGKLLRTL----TGHTGAVRSVAFS 213
WD40 smart00320
WD40 repeats; Note that these repeats are permuted with respect to the structural repeats ...
 155-184 5.40e-03

WD40 repeats; Note that these repeats are permuted with respect to the structural repeats (blades) of the beta propeller domain.


Pssm-ID: 197651 [Multi-domain]  Cd Length: 40  Bit Score: 34.21  E-value: 5.40e-03
                           10        20        30
                   ....*....|....*....|....*....|
gi 1622949777  155 HDSAVTSVRILQDEQYLMASDMAGKIKLWD 184
Cdd:smart00320  11 HTGPVTSVAFSPDGKYLASGSDDGTIKLWD 40
WD40 pfam00400
WD domain, G-beta repeat;
155-184 6.12e-03

WD domain, G-beta repeat;


Pssm-ID: 459801 [Multi-domain]  Cd Length: 39  Bit Score: 33.86  E-value: 6.12e-03
                          10        20        30
                  ....*....|....*....|....*....|
gi 1622949777 155 HDSAVTSVRILQDEQYLMASDMAGKIKLWD 184
Cdd:pfam00400  10 HTGSVTSLAFSPDGKLLASGSDDGTVKVWD 39
 
Name Accession Description Interval E-value
WD40 cd00200
WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions ...
 155-255 2.56e-11

WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from its N-terminus and the WD dipeptide at its C-terminus and is 40 residues long, hence the name WD40; between GH and WD lies a conserved core; serves as a stable propeller-like platform to which proteins can bind either stably or reversibly; forms a propeller-like structure with several blades where each blade is composed of a four-stranded anti-parallel b-sheet; instances with few detectable copies are hypothesized to form larger structures by dimerization; each WD40 sequence repeat forms the first three strands of one blade and the last strand in the next blade; the last C-terminal WD40 repeat completes the blade structure of the first WD40 repeat to create the closed ring propeller-structure; residues on the top and bottom surface of the propeller are proposed to coordinate interactions with other proteins and/or small ligands; 7 copies of the repeat are present in this alignment.


Pssm-ID: 238121 [Multi-domain]  Cd Length: 289  Bit Score: 62.74  E-value: 2.56e-11
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1622949777 155 HDSAVTSVRILQDEQYLMASDMAGKIKLWDLRTTKCIRQYEGH---VNEyaylpLHVHEEEGILVAVGQDCYTRIWSLHD 231
Cdd:cd00200    92 HTSYVSSVAFSPDGRILSSSSRDKTIKVWDVETGKCLTTLRGHtdwVNS-----VAFSPDGTFVASSSQDGTIKLWDLRT 166

                          90       100
                  ....*....|....*....|....
gi 1622949777 232 AHLLRTIpspyPASKADIPSVAFS 255
Cdd:cd00200   167 GKCVATL----TGHTGEVNSVAFS 186
WD40 COG2319
WD40 repeat [General function prediction only];
71-255 1.27e-10

WD40 repeat [General function prediction only];


Pssm-ID: 441893 [Multi-domain]  Cd Length: 403  Bit Score: 61.08  E-value: 1.27e-10
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1622949777  71 AWSCAWSLNIQANNCFSTGLSRRVLLTNVVTGHRQSFGTNSDVLAQQFALMVPLLFNGCRSGEIFAIDLrcgnQGKGWKA 150
Cdd:COG2319    39 VASLAASPDGARLAAGAGDLTLLLLDAAAGALLATLLGHTAAVLSVAFSPDGRLLASASADGTVRLWDL----ATGLLLR 114

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1622949777 151 TRLFHDSAVTSVRILQDEQYLMASDMAGKIKLWDLRTTKCIRQYEGH---VNEYAYLPlhvheeEG-ILVAVGQDCYTRI 226
Cdd:COG2319   115 TLTGHTGAVRSVAFSPDGKTLASGSADGTVRLWDLATGKLLRTLTGHsgaVTSVAFSP------DGkLLASGSDDGTVRL 188

                         170       180
                  ....*....|....*....|....*....
gi 1622949777 227 WSLHDAHLLRTIpspyPASKADIPSVAFS 255
Cdd:COG2319   189 WDLATGKLLRTL----TGHTGAVRSVAFS 213
WD40 COG2319
WD40 repeat [General function prediction only];
155-255 1.57e-10

WD40 repeat [General function prediction only];


Pssm-ID: 441893 [Multi-domain]  Cd Length: 403  Bit Score: 61.08  E-value: 1.57e-10
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1622949777 155 HDSAVTSVRILQDEQYLMASDMAGKIKLWDLRTTKCIRQYEGHVNeyAYLPLHVHEEEGILVAVGQDCYTRIWSLHDAHL 234
Cdd:COG2319   287 HSGGVNSVAFSPDGKLLASGSDDGTVRLWDLATGKLLRTLTGHTG--AVRSVAFSPDGKTLASGSDDGTVRLWDLATGEL 364

                          90       100
                  ....*....|....*....|.
gi 1622949777 235 LRTIpspyPASKADIPSVAFS 255
Cdd:COG2319   365 LRTL----TGHTGAVTSVAFS 381
WD40 COG2319
WD40 repeat [General function prediction only];
155-255 1.62e-10

WD40 repeat [General function prediction only];


Pssm-ID: 441893 [Multi-domain]  Cd Length: 403  Bit Score: 61.08  E-value: 1.62e-10
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1622949777 155 HDSAVTSVRILQDEQYLMASDMAGKIKLWDLRTTKCIRQYEGH---VNEYAYLPlhvheeEG-ILVAVGQDCYTRIWSLH 230
Cdd:COG2319   245 HSGSVRSVAFSPDGRLLASGSADGTVRLWDLATGELLRTLTGHsggVNSVAFSP------DGkLLASGSDDGTVRLWDLA 318

                          90       100
                  ....*....|....*....|....*
gi 1622949777 231 DAHLLRTIpspyPASKADIPSVAFS 255
Cdd:COG2319   319 TGKLLRTL----TGHTGAVRSVAFS 339
WD40 COG2319
WD40 repeat [General function prediction only];
155-255 2.68e-10

WD40 repeat [General function prediction only];


Pssm-ID: 441893 [Multi-domain]  Cd Length: 403  Bit Score: 60.31  E-value: 2.68e-10
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1622949777 155 HDSAVTSVRILQDEQYLMASDMAGKIKLWDLRTTKCIRQYEGH---VNEYAYLPlhvheeEG-ILVAVGQDCYTRIWSLH 230
Cdd:COG2319   161 HSGAVTSVAFSPDGKLLASGSDDGTVRLWDLATGKLLRTLTGHtgaVRSVAFSP------DGkLLASGSADGTVRLWDLA 234

                          90       100
                  ....*....|....*....|....*
gi 1622949777 231 DAHLLRTIPSPypasKADIPSVAFS 255
Cdd:COG2319   235 TGKLLRTLTGH----SGSVRSVAFS 255
WD40 cd00200
WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions ...
 149-256 5.21e-10

WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from its N-terminus and the WD dipeptide at its C-terminus and is 40 residues long, hence the name WD40; between GH and WD lies a conserved core; serves as a stable propeller-like platform to which proteins can bind either stably or reversibly; forms a propeller-like structure with several blades where each blade is composed of a four-stranded anti-parallel b-sheet; instances with few detectable copies are hypothesized to form larger structures by dimerization; each WD40 sequence repeat forms the first three strands of one blade and the last strand in the next blade; the last C-terminal WD40 repeat completes the blade structure of the first WD40 repeat to create the closed ring propeller-structure; residues on the top and bottom surface of the propeller are proposed to coordinate interactions with other proteins and/or small ligands; 7 copies of the repeat are present in this alignment.


Pssm-ID: 238121 [Multi-domain]  Cd Length: 289  Bit Score: 58.89  E-value: 5.21e-10
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1622949777 149 KATRLFHDSAVTSVRILQDEQYLMASDMAGKIKLWDLRTTKCIRQYEGHVNE-YAylpLHVHEEEGILVAVGQDCYTRIW 227
Cdd:cd00200   128 LTTLRGHTDWVNSVAFSPDGTFVASSSQDGTIKLWDLRTGKCVATLTGHTGEvNS---VAFSPDGEKLLSSSSDGTIKLW 204

                          90       100
                  ....*....|....*....|....*....
gi 1622949777 228 SLHDAHLLRTipspYPASKADIPSVAFSS 256
Cdd:cd00200   205 DLSTGKCLGT----LRGHENGVNSVAFSP 229
WD40 cd00200
WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions ...
 125-228 6.23e-10

WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from its N-terminus and the WD dipeptide at its C-terminus and is 40 residues long, hence the name WD40; between GH and WD lies a conserved core; serves as a stable propeller-like platform to which proteins can bind either stably or reversibly; forms a propeller-like structure with several blades where each blade is composed of a four-stranded anti-parallel b-sheet; instances with few detectable copies are hypothesized to form larger structures by dimerization; each WD40 sequence repeat forms the first three strands of one blade and the last strand in the next blade; the last C-terminal WD40 repeat completes the blade structure of the first WD40 repeat to create the closed ring propeller-structure; residues on the top and bottom surface of the propeller are proposed to coordinate interactions with other proteins and/or small ligands; 7 copies of the repeat are present in this alignment.


Pssm-ID: 238121 [Multi-domain]  Cd Length: 289  Bit Score: 58.50  E-value: 6.23e-10
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1622949777 125 LFNGCRSGEIFAIDLRCGNqgkgWKATRLFHDSAVTSVRILQDEQYLMASDMAGKIKLWDLRTTKCIRQYEGHVNE-YAy 203
Cdd:cd00200   192 LLSSSSDGTIKLWDLSTGK----CLGTLRGHENGVNSVAFSPDGYLLASGSEDGTIRVWDLRTGECVQTLSGHTNSvTS- 266

                          90       100
                  ....*....|....*....|....*
gi 1622949777 204 lpLHVHEEEGILVAVGQDCYTRIWS 228
Cdd:cd00200   267 --LAWSPDGKRLASGSADGTIRIWD 289
WD40 cd00200
WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions ...
 155-255 4.75e-08

WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from its N-terminus and the WD dipeptide at its C-terminus and is 40 residues long, hence the name WD40; between GH and WD lies a conserved core; serves as a stable propeller-like platform to which proteins can bind either stably or reversibly; forms a propeller-like structure with several blades where each blade is composed of a four-stranded anti-parallel b-sheet; instances with few detectable copies are hypothesized to form larger structures by dimerization; each WD40 sequence repeat forms the first three strands of one blade and the last strand in the next blade; the last C-terminal WD40 repeat completes the blade structure of the first WD40 repeat to create the closed ring propeller-structure; residues on the top and bottom surface of the propeller are proposed to coordinate interactions with other proteins and/or small ligands; 7 copies of the repeat are present in this alignment.


Pssm-ID: 238121 [Multi-domain]  Cd Length: 289  Bit Score: 53.11  E-value: 4.75e-08
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1622949777 155 HDSAVTSVRILQDEQYLMASDMAGKIKLWDLRTTKCIRQYEGHVNEYAYLPLHVHEEEgiLVAVGQDCYTRIWSLHDAHL 234
Cdd:cd00200     8 HTGGVTCVAFSPDGKLLATGSGDGTIKVWDLETGELLRTLKGHTGPVRDVAASADGTY--LASGSSDKTIRLWDLETGEC 85

                          90       100
                  ....*....|....*....|.
gi 1622949777 235 LRTipspYPASKADIPSVAFS 255
Cdd:cd00200    86 VRT----LTGHTSYVSSVAFS 102
WD40 smart00320
WD40 repeats; Note that these repeats are permuted with respect to the structural repeats ...
 155-184 5.40e-03

WD40 repeats; Note that these repeats are permuted with respect to the structural repeats (blades) of the beta propeller domain.


Pssm-ID: 197651 [Multi-domain]  Cd Length: 40  Bit Score: 34.21  E-value: 5.40e-03
                           10        20        30
                   ....*....|....*....|....*....|
gi 1622949777  155 HDSAVTSVRILQDEQYLMASDMAGKIKLWD 184
Cdd:smart00320  11 HTGPVTSVAFSPDGKYLASGSDDGTIKLWD 40
WD40 COG2319
WD40 repeat [General function prediction only];
150-255 5.46e-03

WD40 repeat [General function prediction only];


Pssm-ID: 441893 [Multi-domain]  Cd Length: 403  Bit Score: 37.97  E-value: 5.46e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1622949777 150 ATRLFHDSAVTSVRILQDEQYLMASDMAGKIKLWDLRTTKCIRQYEGHVNeyAYLPLHVHEEEGILVAVGQDCYTRIWSL 229
Cdd:COG2319    30 LLLLGLAAAVASLAASPDGARLAAGAGDLTLLLLDAAAGALLATLLGHTA--AVLSVAFSPDGRLLASASADGTVRLWDL 107

                          90       100
                  ....*....|....*....|....*.
gi 1622949777 230 HDAHLLRTIpspyPASKADIPSVAFS 255
Cdd:COG2319   108 ATGLLLRTL----TGHTGAVRSVAFS 129
WD40 pfam00400
WD domain, G-beta repeat;
155-184 6.12e-03

WD domain, G-beta repeat;


Pssm-ID: 459801 [Multi-domain]  Cd Length: 39  Bit Score: 33.86  E-value: 6.12e-03
                          10        20        30
                  ....*....|....*....|....*....|
gi 1622949777 155 HDSAVTSVRILQDEQYLMASDMAGKIKLWD 184
Cdd:pfam00400  10 HTGSVTSLAFSPDGKLLASGSDDGTVKVWD 39
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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