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Conserved domains on  [gi|1227964537|ref|XP_021913577|]
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protein inscuteable homolog [Zootermopsis nevadensis]

Protein Classification

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
INSC_LBD cd21966
LGN-binding domain (LBD) of protein Inscuteable (INSC) and similar proteins; Inscuteable (INSC) ...
 463-756 1.77e-117

LGN-binding domain (LBD) of protein Inscuteable (INSC) and similar proteins; Inscuteable (INSC) functions as an adapter protein linking the Par polarity complex (composed of Par3, Par6 and protein kinase C iota) to the Gpsm1/Gpsm2 complex, and is involved in cell polarity and spindle orientation during mitosis. Par3 interacts with INSC which binds directly to Pins (partner of Insc, homolog of vertebrate LGN/Gpsm2 and AGS3/Gpsm1). Pins then associates with the heterotrimeric G proteins and NuMA, which interacts directly with the cell spindle to control the orientation of the spindle and the division plane of mitotic cells. INSC may regulate cell proliferation and differentiation in the developing nervous system, may play a role in the asymmetric division of fibroblasts, and may also participate in the process of stratification of the squamous epithelium. This model corresponds to the Leu-Gly-Asn Repeat-Enriched Protein (LGN)-binding domain (LBD) of INSC. It interacts with the tetratricopeptide repeat (TPR) motifs of LGN, also called G-protein-signaling modulator 2 (Gpsm2).


:

Pssm-ID: 439320 [Multi-domain]  Cd Length: 300  Bit Score: 361.19  E-value: 1.77e-117
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1227964537 463 PVQRWLGELATLCEPECMTTLQSKSLAVDLSHQVAMMAAATTDTVKMLQEGTKLISTEFAKLCQQLDYGKMEHVGPLVQS 542
Cdd:cd21966     4 SVQQWLSELRLMTEPECMTTLQSKSIAGEEDQKVKLLVSSATDSIRKLQQRAHIISAEFDKLYRKLEKGRWKQVHPLIQS 83

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1227964537 543 LVGLVTEFIHEHCNKQEETRKRSQTEQETLTEVCDRLRITAAR---HPPNKEELTSEITELGNNFTNIVDNILTHHISVL 619
Cdd:cd21966    84 LTGHIRSLLQEYNATLPELPPELQQYEQRLLEICEELRTAAERslgSKPNLEELLKLLTTLGQSFSKLVDLLLLKELQIL 163

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1227964537 620 VEVLEEPTSDMALRSALSSLTAFGLEGPHLSRLVAHCSGVRALLSICLESRSSTIRTAALRALATVCCVAEAIRQLEQAG 699
Cdd:cd21966   164 VDSLEEPTSELSLRAALSALTSLGLEGPHLCRLIAKEGGVRALLIVCRDCKSSSVRALALRALATVCCVPEAIRQFEKAG 243

                         250       260       270       280       290
                  ....*....|....*....|....*....|....*....|....*....|....*..
gi 1227964537 700 GVEILSEMLSEEARPEPEVSEAAAVLAQITAPWVEDNHTVHGLSEQLSSLVHSLTRL 756
Cdd:cd21966   244 GIEILSDILTDESRSEPERSEAAGVLAQITSPWIDDNHHLSGLIEHMEDLVKALTRL 300
INSC_LBD super family cl41917
LGN-binding domain (LBD) of protein Inscuteable (INSC) and similar proteins; Inscuteable (INSC) ...
 618-958 5.66e-60

LGN-binding domain (LBD) of protein Inscuteable (INSC) and similar proteins; Inscuteable (INSC) functions as an adapter protein linking the Par polarity complex (composed of Par3, Par6 and protein kinase C iota) to the Gpsm1/Gpsm2 complex, and is involved in cell polarity and spindle orientation during mitosis. Par3 interacts with INSC which binds directly to Pins (partner of Insc, homolog of vertebrate LGN/Gpsm2 and AGS3/Gpsm1). Pins then associates with the heterotrimeric G proteins and NuMA, which interacts directly with the cell spindle to control the orientation of the spindle and the division plane of mitotic cells. INSC may regulate cell proliferation and differentiation in the developing nervous system, may play a role in the asymmetric division of fibroblasts, and may also participate in the process of stratification of the squamous epithelium. This model corresponds to the Leu-Gly-Asn Repeat-Enriched Protein (LGN)-binding domain (LBD) of INSC. It interacts with the tetratricopeptide repeat (TPR) motifs of LGN, also called G-protein-signaling modulator 2 (Gpsm2).


The actual alignment was detected with superfamily member pfam19427:

Pssm-ID: 477865  Cd Length: 394  Bit Score: 210.15  E-value: 5.66e-60
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1227964537 618 VLVEVLEEPTSDMALRSALSSLTAFGLEGPHLSRLVAHCSGVRALLSICLESRSSTIRTAALRALATVCCVAEAIRQLEQ 697
Cdd:pfam19427  56 ILVRQIESSDNIYTIESAISSLFSLTQEGAQLCSIIAKEGGVVALFKICRQDCFRCLYPHALRTLASICCVEEGINQLEK 135

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1227964537 698 AGGVEILSEMLSEEARPEPEVSEAAAVLAQITAPWVEDNHTVHGLSEQLSSLVHSLTRLASTTASCETLLLSAAALANLT 777
Cdd:pfam19427 136 VDGILCLADILTDETHSEATRAEAAAVVAQITSPHLPFTQHLSSFLENMEDIVTALIKLCQEASSGEVFLLASAALANIT 215

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1227964537 778 FMEPRTVWPLLEQGTAGKLLQAVKRQGPKVSVFLQEQAATLIANMAAVPESRPHLAEQRAVVALLCFLQIRHSPlQRAPE 857
Cdd:pfam19427 216 FFDTMACEMLLQLNAMKILLEACTDKQRVDTPYSRDQVVTILANMSVLEQCASDIIQENGIELLMEMLSEKPSS-GSPAE 294

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1227964537 858 IAAAERVQQKSAIALSRLCSDPVVAAQVVELQGVNRLVRLCKEERERNHSDGVLVACLAALRKISANCgTKVIEDLDAME 937
Cdd:pfam19427 295 VAACERVQQKAAVTLARLSRDPDVARTAIKLNCIPRLIELCRSPAERNNSDSVLVACLAALRRLAAMC-PEGLEDSDFQQ 373

                         330       340
                  ....*....|....*....|.
gi 1227964537 938 LVEPRLLDSFLIYSSRQESYV 958
Cdd:pfam19427 374 LVKPRLVDSFLLCSNMEESFV 394
 
Name Accession Description Interval E-value
INSC_LBD cd21966
LGN-binding domain (LBD) of protein Inscuteable (INSC) and similar proteins; Inscuteable (INSC) ...
 463-756 1.77e-117

LGN-binding domain (LBD) of protein Inscuteable (INSC) and similar proteins; Inscuteable (INSC) functions as an adapter protein linking the Par polarity complex (composed of Par3, Par6 and protein kinase C iota) to the Gpsm1/Gpsm2 complex, and is involved in cell polarity and spindle orientation during mitosis. Par3 interacts with INSC which binds directly to Pins (partner of Insc, homolog of vertebrate LGN/Gpsm2 and AGS3/Gpsm1). Pins then associates with the heterotrimeric G proteins and NuMA, which interacts directly with the cell spindle to control the orientation of the spindle and the division plane of mitotic cells. INSC may regulate cell proliferation and differentiation in the developing nervous system, may play a role in the asymmetric division of fibroblasts, and may also participate in the process of stratification of the squamous epithelium. This model corresponds to the Leu-Gly-Asn Repeat-Enriched Protein (LGN)-binding domain (LBD) of INSC. It interacts with the tetratricopeptide repeat (TPR) motifs of LGN, also called G-protein-signaling modulator 2 (Gpsm2).


Pssm-ID: 439320 [Multi-domain]  Cd Length: 300  Bit Score: 361.19  E-value: 1.77e-117
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1227964537 463 PVQRWLGELATLCEPECMTTLQSKSLAVDLSHQVAMMAAATTDTVKMLQEGTKLISTEFAKLCQQLDYGKMEHVGPLVQS 542
Cdd:cd21966     4 SVQQWLSELRLMTEPECMTTLQSKSIAGEEDQKVKLLVSSATDSIRKLQQRAHIISAEFDKLYRKLEKGRWKQVHPLIQS 83

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1227964537 543 LVGLVTEFIHEHCNKQEETRKRSQTEQETLTEVCDRLRITAAR---HPPNKEELTSEITELGNNFTNIVDNILTHHISVL 619
Cdd:cd21966    84 LTGHIRSLLQEYNATLPELPPELQQYEQRLLEICEELRTAAERslgSKPNLEELLKLLTTLGQSFSKLVDLLLLKELQIL 163

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1227964537 620 VEVLEEPTSDMALRSALSSLTAFGLEGPHLSRLVAHCSGVRALLSICLESRSSTIRTAALRALATVCCVAEAIRQLEQAG 699
Cdd:cd21966   164 VDSLEEPTSELSLRAALSALTSLGLEGPHLCRLIAKEGGVRALLIVCRDCKSSSVRALALRALATVCCVPEAIRQFEKAG 243

                         250       260       270       280       290
                  ....*....|....*....|....*....|....*....|....*....|....*..
gi 1227964537 700 GVEILSEMLSEEARPEPEVSEAAAVLAQITAPWVEDNHTVHGLSEQLSSLVHSLTRL 756
Cdd:cd21966   244 GIEILSDILTDESRSEPERSEAAGVLAQITSPWIDDNHHLSGLIEHMEDLVKALTRL 300
Insc_C pfam19427
Protein inscuteable C-terminal; This entry represents the C-terminal region of protein ...
 618-958 5.66e-60

Protein inscuteable C-terminal; This entry represents the C-terminal region of protein Inscuteable (Insc) containing four putative armadillo repeats and the conserved extreme C-terminal tetrapeptide Glu-Ser-Phe-Val which is in agreement with the class I PDZ-binding motif X-Ser/Thr-X-/ (X and /are unspecified and hydrophobic residues, respectively). Insc functions as an adaptor protein that binds simultaneously with Partner of Insc (Pins, consisting of alpha subunit (Ga) of heterotrimeric G proteins and its guanine nucleotide dissociation inhibitor) and Par3 (PDZ-containing proteins) to regulate their apical localization in neuroblasts, which is required for the correct execution of the asymmetric division.


Pssm-ID: 466076  Cd Length: 394  Bit Score: 210.15  E-value: 5.66e-60
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1227964537 618 VLVEVLEEPTSDMALRSALSSLTAFGLEGPHLSRLVAHCSGVRALLSICLESRSSTIRTAALRALATVCCVAEAIRQLEQ 697
Cdd:pfam19427  56 ILVRQIESSDNIYTIESAISSLFSLTQEGAQLCSIIAKEGGVVALFKICRQDCFRCLYPHALRTLASICCVEEGINQLEK 135

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1227964537 698 AGGVEILSEMLSEEARPEPEVSEAAAVLAQITAPWVEDNHTVHGLSEQLSSLVHSLTRLASTTASCETLLLSAAALANLT 777
Cdd:pfam19427 136 VDGILCLADILTDETHSEATRAEAAAVVAQITSPHLPFTQHLSSFLENMEDIVTALIKLCQEASSGEVFLLASAALANIT 215

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1227964537 778 FMEPRTVWPLLEQGTAGKLLQAVKRQGPKVSVFLQEQAATLIANMAAVPESRPHLAEQRAVVALLCFLQIRHSPlQRAPE 857
Cdd:pfam19427 216 FFDTMACEMLLQLNAMKILLEACTDKQRVDTPYSRDQVVTILANMSVLEQCASDIIQENGIELLMEMLSEKPSS-GSPAE 294

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1227964537 858 IAAAERVQQKSAIALSRLCSDPVVAAQVVELQGVNRLVRLCKEERERNHSDGVLVACLAALRKISANCgTKVIEDLDAME 937
Cdd:pfam19427 295 VAACERVQQKAAVTLARLSRDPDVARTAIKLNCIPRLIELCRSPAERNNSDSVLVACLAALRRLAAMC-PEGLEDSDFQQ 373

                         330       340
                  ....*....|....*....|.
gi 1227964537 938 LVEPRLLDSFLIYSSRQESYV 958
Cdd:pfam19427 374 LVKPRLVDSFLLCSNMEESFV 394
INSC_LBD pfam16748
Inscuteable LGN-binding domain; This is the LGN-binding domain (LBD) of the inscuteable ...
 464-491 3.12e-03

Inscuteable LGN-binding domain; This is the LGN-binding domain (LBD) of the inscuteable homolog protein. It interacts with the TPR motifs of G-protein-signaling modulator 2 (GPSM2) (LGN) and stabilizes LGN.


Pssm-ID: 465257  Cd Length: 47  Bit Score: 36.44  E-value: 3.12e-03
                          10        20
                  ....*....|....*....|....*...
gi 1227964537 464 VQRWLGELATLCEPECMTTLQSKSLAVD 491
Cdd:pfam16748   6 VQRWMEDLRLMTEVECMCVLQSKPISVE 33
 
Name Accession Description Interval E-value
INSC_LBD cd21966
LGN-binding domain (LBD) of protein Inscuteable (INSC) and similar proteins; Inscuteable (INSC) ...
 463-756 1.77e-117

LGN-binding domain (LBD) of protein Inscuteable (INSC) and similar proteins; Inscuteable (INSC) functions as an adapter protein linking the Par polarity complex (composed of Par3, Par6 and protein kinase C iota) to the Gpsm1/Gpsm2 complex, and is involved in cell polarity and spindle orientation during mitosis. Par3 interacts with INSC which binds directly to Pins (partner of Insc, homolog of vertebrate LGN/Gpsm2 and AGS3/Gpsm1). Pins then associates with the heterotrimeric G proteins and NuMA, which interacts directly with the cell spindle to control the orientation of the spindle and the division plane of mitotic cells. INSC may regulate cell proliferation and differentiation in the developing nervous system, may play a role in the asymmetric division of fibroblasts, and may also participate in the process of stratification of the squamous epithelium. This model corresponds to the Leu-Gly-Asn Repeat-Enriched Protein (LGN)-binding domain (LBD) of INSC. It interacts with the tetratricopeptide repeat (TPR) motifs of LGN, also called G-protein-signaling modulator 2 (Gpsm2).


Pssm-ID: 439320 [Multi-domain]  Cd Length: 300  Bit Score: 361.19  E-value: 1.77e-117
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1227964537 463 PVQRWLGELATLCEPECMTTLQSKSLAVDLSHQVAMMAAATTDTVKMLQEGTKLISTEFAKLCQQLDYGKMEHVGPLVQS 542
Cdd:cd21966     4 SVQQWLSELRLMTEPECMTTLQSKSIAGEEDQKVKLLVSSATDSIRKLQQRAHIISAEFDKLYRKLEKGRWKQVHPLIQS 83

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1227964537 543 LVGLVTEFIHEHCNKQEETRKRSQTEQETLTEVCDRLRITAAR---HPPNKEELTSEITELGNNFTNIVDNILTHHISVL 619
Cdd:cd21966    84 LTGHIRSLLQEYNATLPELPPELQQYEQRLLEICEELRTAAERslgSKPNLEELLKLLTTLGQSFSKLVDLLLLKELQIL 163

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1227964537 620 VEVLEEPTSDMALRSALSSLTAFGLEGPHLSRLVAHCSGVRALLSICLESRSSTIRTAALRALATVCCVAEAIRQLEQAG 699
Cdd:cd21966   164 VDSLEEPTSELSLRAALSALTSLGLEGPHLCRLIAKEGGVRALLIVCRDCKSSSVRALALRALATVCCVPEAIRQFEKAG 243

                         250       260       270       280       290
                  ....*....|....*....|....*....|....*....|....*....|....*..
gi 1227964537 700 GVEILSEMLSEEARPEPEVSEAAAVLAQITAPWVEDNHTVHGLSEQLSSLVHSLTRL 756
Cdd:cd21966   244 GIEILSDILTDESRSEPERSEAAGVLAQITSPWIDDNHHLSGLIEHMEDLVKALTRL 300
Insc_C pfam19427
Protein inscuteable C-terminal; This entry represents the C-terminal region of protein ...
 618-958 5.66e-60

Protein inscuteable C-terminal; This entry represents the C-terminal region of protein Inscuteable (Insc) containing four putative armadillo repeats and the conserved extreme C-terminal tetrapeptide Glu-Ser-Phe-Val which is in agreement with the class I PDZ-binding motif X-Ser/Thr-X-/ (X and /are unspecified and hydrophobic residues, respectively). Insc functions as an adaptor protein that binds simultaneously with Partner of Insc (Pins, consisting of alpha subunit (Ga) of heterotrimeric G proteins and its guanine nucleotide dissociation inhibitor) and Par3 (PDZ-containing proteins) to regulate their apical localization in neuroblasts, which is required for the correct execution of the asymmetric division.


Pssm-ID: 466076  Cd Length: 394  Bit Score: 210.15  E-value: 5.66e-60
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1227964537 618 VLVEVLEEPTSDMALRSALSSLTAFGLEGPHLSRLVAHCSGVRALLSICLESRSSTIRTAALRALATVCCVAEAIRQLEQ 697
Cdd:pfam19427  56 ILVRQIESSDNIYTIESAISSLFSLTQEGAQLCSIIAKEGGVVALFKICRQDCFRCLYPHALRTLASICCVEEGINQLEK 135

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1227964537 698 AGGVEILSEMLSEEARPEPEVSEAAAVLAQITAPWVEDNHTVHGLSEQLSSLVHSLTRLASTTASCETLLLSAAALANLT 777
Cdd:pfam19427 136 VDGILCLADILTDETHSEATRAEAAAVVAQITSPHLPFTQHLSSFLENMEDIVTALIKLCQEASSGEVFLLASAALANIT 215

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1227964537 778 FMEPRTVWPLLEQGTAGKLLQAVKRQGPKVSVFLQEQAATLIANMAAVPESRPHLAEQRAVVALLCFLQIRHSPlQRAPE 857
Cdd:pfam19427 216 FFDTMACEMLLQLNAMKILLEACTDKQRVDTPYSRDQVVTILANMSVLEQCASDIIQENGIELLMEMLSEKPSS-GSPAE 294

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1227964537 858 IAAAERVQQKSAIALSRLCSDPVVAAQVVELQGVNRLVRLCKEERERNHSDGVLVACLAALRKISANCgTKVIEDLDAME 937
Cdd:pfam19427 295 VAACERVQQKAAVTLARLSRDPDVARTAIKLNCIPRLIELCRSPAERNNSDSVLVACLAALRRLAAMC-PEGLEDSDFQQ 373

                         330       340
                  ....*....|....*....|.
gi 1227964537 938 LVEPRLLDSFLIYSSRQESYV 958
Cdd:pfam19427 374 LVKPRLVDSFLLCSNMEESFV 394
INSC_LBD pfam16748
Inscuteable LGN-binding domain; This is the LGN-binding domain (LBD) of the inscuteable ...
 464-491 3.12e-03

Inscuteable LGN-binding domain; This is the LGN-binding domain (LBD) of the inscuteable homolog protein. It interacts with the TPR motifs of G-protein-signaling modulator 2 (GPSM2) (LGN) and stabilizes LGN.


Pssm-ID: 465257  Cd Length: 47  Bit Score: 36.44  E-value: 3.12e-03
                          10        20
                  ....*....|....*....|....*...
gi 1227964537 464 VQRWLGELATLCEPECMTTLQSKSLAVD 491
Cdd:pfam16748   6 VQRWMEDLRLMTEVECMCVLQSKPISVE 33
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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