|
Name |
Accession |
Description |
Interval |
E-value |
| PLN02654 |
PLN02654 |
acetate-CoA ligase |
42-707 |
0e+00 |
|
acetate-CoA ligase
Pssm-ID: 215353 [Multi-domain] Cd Length: 666 Bit Score: 1469.36 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 42 VLGESLAAEENDLIFPSSDFSANALVSSPKQYREMYERSIKNPAGFWSEIAETFYWKEKWNPAEVCSENLDVTKGPVQIN 121
Cdd:PLN02654 1 VLGESLASEENDLVFPSKDFSAQALVSSPQQYMEMYKRSVDDPAGFWSDIASQFYWKQKWEGDEVCSENLDVRKGPISIE 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 122 WFKGGKTNICYNAVDRNIESGNGDKIAMYWEGNEPSQDGKLTYSELLEKVCQLANYLKSLGVGKGDAVIIYLPMLLELPI 201
Cdd:PLN02654 81 WFKGGKTNICYNCLDRNVEAGNGDKIAIYWEGNEPGFDASLTYSELLDRVCQLANYLKDVGVKKGDAVVIYLPMLMELPI 160
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 202 AMLACARIGAVHSVVFAGFSADSLAQRIVDCKPKLVITCNAVKRGVKPIFLKDIVDVALVESEKNGVSVGLCLTYENQSA 281
Cdd:PLN02654 161 AMLACARIGAVHSVVFAGFSAESLAQRIVDCKPKVVITCNAVKRGPKTINLKDIVDAALDESAKNGVSVGICLTYENQLA 240
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 282 MKREDTKWQPERDVWWQDVVTKFPTKCDVEWVDAEDPLFLLYTSGSTGKPKGVLHTSGGYMVYTATTFKYAFDYKPTDIY 361
Cdd:PLN02654 241 MKREDTKWQEGRDVWWQDVVPNYPTKCEVEWVDAEDPLFLLYTSGSTGKPKGVLHTTGGYMVYTATTFKYAFDYKPTDVY 320
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 362 WCTADCGWITGHSYVTYGPLLNGATVLVFEGTPNYPDSGRCWDIVDKYNVTIFYTAPTLVRSLMRDGTEYVTRYSRKSLR 441
Cdd:PLN02654 321 WCTADCGWITGHSYVTYGPMLNGATVLVFEGAPNYPDSGRCWDIVDKYKVTIFYTAPTLVRSLMRDGDEYVTRHSRKSLR 400
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 442 VLGSVGEPINPSAWRWFYNIVGDSRCPISDTWWQTETGGFMITPLPGAWPQKPGSATFPFFGVQPVIVDEKGQEIEGECS 521
Cdd:PLN02654 401 VLGSVGEPINPSAWRWFFNVVGDSRCPISDTWWQTETGGFMITPLPGAWPQKPGSATFPFFGVQPVIVDEKGKEIEGECS 480
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 522 GYLCIKKSWPGAFRTLYGDHERYETTYFKPFAGYYFTGDGCSRDKDGYHWLTGRVDDVINVSGHRIGTAEVESALVSHPQ 601
Cdd:PLN02654 481 GYLCVKKSWPGAFRTLYGDHERYETTYFKPFAGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVSHPQ 560
|
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 602 CAEAAVVGVEHEVKGQGIYAFVTLVDGVPYSEELRKSLIMTVRNQIGAFAAPDKIHWAPGLPKTRSGKIMRRILRKIAAR 681
Cdd:PLN02654 561 CAEAAVVGIEHEVKGQGIYAFVTLVEGVPYSEELRKSLILTVRNQIGAFAAPDKIHWAPGLPKTRSGKIMRRILRKIASR 640
|
650 660
....*....|....*....|....*.
gi 242061796 682 QLDELGDISTLAEPAVVDQLIALSDC 707
Cdd:PLN02654 641 QLDELGDTSTLADPGVVDQLIALADS 666
|
|
| ACS |
cd05966 |
Acetyl-CoA synthetase (also known as acetate-CoA ligase and acetyl-activating enzyme); ... |
72-694 |
0e+00 |
|
Acetyl-CoA synthetase (also known as acetate-CoA ligase and acetyl-activating enzyme); Acetyl-CoA synthetase (ACS, EC 6.2.1.1, acetate#CoA ligase or acetate:CoA ligase (AMP-forming)) catalyzes the formation of acetyl-CoA from acetate, CoA, and ATP. Synthesis of acetyl-CoA is carried out in a two-step reaction. In the first step, the enzyme catalyzes the synthesis of acetyl-AMP intermediate from acetate and ATP. In the second step, acetyl-AMP reacts with CoA to produce acetyl-CoA. This enzyme is widely present in all living organisms. The activity of this enzyme is crucial for maintaining the required levels of acetyl-CoA, a key intermediate in many important biosynthetic and catabolic processes. Acetyl-CoA is used in the biosynthesis of glucose, fatty acids, and cholesterol. It can also be used in the production of energy in the citric acid cycle. Eukaryotes typically have two isoforms of acetyl-CoA synthetase, a cytosolic form involved in biosynthetic processes and a mitochondrial form primarily involved in energy generation.
Pssm-ID: 341270 [Multi-domain] Cd Length: 608 Bit Score: 1153.08 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 72 QYREMYERSIKNPAGFWSEIAETFYWKEKWnpaevcSENLDVTKGPVQINWFKGGKTNICYNAVDRNIESgNGDKIAMYW 151
Cdd:cd05966 2 QYKELYKQSIEDPEEFWGEIAKELDWFKPW------DKVLDWSKGPPFIKWFEGGKLNISYNCLDRHLKE-RGDKVAIIW 74
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 152 EGNEPSQDGKLTYSELLEKVCQLANYLKSLGVGKGDAVIIYLPMLLELPIAMLACARIGAVHSVVFAGFSADSLAQRIVD 231
Cdd:cd05966 75 EGDEPDQSRTITYRELLREVCRFANVLKSLGVKKGDRVAIYMPMIPELVIAMLACARIGAVHSVVFAGFSAESLADRIND 154
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 232 CKPKLVITCNAVKRGVKPIFLKDIVDVALveseKNGVSVGLCLTYENQsamkREDTKWQPERDVWWQDVVTKFPTKCDVE 311
Cdd:cd05966 155 AQCKLVITADGGYRGGKVIPLKEIVDEAL----EKCPSVEKVLVVKRT----GGEVPMTEGRDLWWHDLMAKQSPECEPE 226
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 312 WVDAEDPLFLLYTSGSTGKPKGVLHTSGGYMVYTATTFKYAFDYKPTDIYWCTADCGWITGHSYVTYGPLLNGATVLVFE 391
Cdd:cd05966 227 WMDSEDPLFILYTSGSTGKPKGVVHTTGGYLLYAATTFKYVFDYHPDDIYWCTADIGWITGHSYIVYGPLANGATTVMFE 306
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 392 GTPNYPDSGRCWDIVDKYNVTIFYTAPTLVRSLMRDGTEYVTRYSRKSLRVLGSVGEPINPSAWRWFYNIVGDSRCPISD 471
Cdd:cd05966 307 GTPTYPDPGRYWDIVEKHKVTIFYTAPTAIRALMKFGDEWVKKHDLSSLRVLGSVGEPINPEAWMWYYEVIGKERCPIVD 386
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 472 TWWQTETGGFMITPLPGAWPQKPGSATFPFFGVQPVIVDEKGQEIEGECSGYLCIKKSWPGAFRTLYGDHERYETTYFKP 551
Cdd:cd05966 387 TWWQTETGGIMITPLPGATPLKPGSATRPFFGIEPAILDEEGNEVEGEVEGYLVIKRPWPGMARTIYGDHERYEDTYFSK 466
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 552 FAGYYFTGDGCSRDKDGYHWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAAVVGVEHEVKGQGIYAFVTLVDGVPY 631
Cdd:cd05966 467 FPGYYFTGDGARRDEDGYYWITGRVDDVINVSGHRLGTAEVESALVAHPAVAEAAVVGRPHDIKGEAIYAFVTLKDGEEP 546
|
570 580 590 600 610 620
....*....|....*....|....*....|....*....|....*....|....*....|...
gi 242061796 632 SEELRKSLIMTVRNQIGAFAAPDKIHWAPGLPKTRSGKIMRRILRKIAARQlDELGDISTLAE 694
Cdd:cd05966 547 SDELRKELRKHVRKEIGPIATPDKIQFVPGLPKTRSGKIMRRILRKIAAGE-EELGDTSTLAD 608
|
|
| PRK00174 |
PRK00174 |
acetyl-CoA synthetase; Provisional |
57-703 |
0e+00 |
|
acetyl-CoA synthetase; Provisional
Pssm-ID: 234677 [Multi-domain] Cd Length: 637 Bit Score: 1139.48 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 57 PSSDFSANALVSsPKQYREMYERSIKNPAGFWSEIAETFYWKEKWNpaevcsENLDvtKGPVQINWFKGGKTNICYNAVD 136
Cdd:PRK00174 4 PPAEFAANALID-MEQYKALYQESVEDPEGFWAEQAKRLDWFKPFD------TVLD--WNAPFIKWFEDGELNVSYNCLD 74
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 137 RNIESGnGDKIAMYWEGNEPSQDGKLTYSELLEKVCQLANYLKSLGVGKGDAVIIYLPMLLELPIAMLACARIGAVHSVV 216
Cdd:PRK00174 75 RHLKTR-GDKVAIIWEGDDPGDSRKITYRELHREVCRFANALKSLGVKKGDRVAIYMPMIPEAAVAMLACARIGAVHSVV 153
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 217 FAGFSADSLAQRIVDCKPKLVITCNAVKRGVKPIFLKDIVDVALVEsekngvsvglCLTYENQSAMKR--EDTKWQPERD 294
Cdd:PRK00174 154 FGGFSAEALADRIIDAGAKLVITADEGVRGGKPIPLKANVDEALAN----------CPSVEKVIVVRRtgGDVDWVEGRD 223
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 295 VWWQDVVTKFPTKCDVEWVDAEDPLFLLYTSGSTGKPKGVLHTSGGYMVYTATTFKYAFDYKPTDIYWCTADCGWITGHS 374
Cdd:PRK00174 224 LWWHELVAGASDECEPEPMDAEDPLFILYTSGSTGKPKGVLHTTGGYLVYAAMTMKYVFDYKDGDVYWCTADVGWVTGHS 303
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 375 YVTYGPLLNGATVLVFEGTPNYPDSGRCWDIVDKYNVTIFYTAPTLVRSLMRDGTEYVTRYSRKSLRVLGSVGEPINPSA 454
Cdd:PRK00174 304 YIVYGPLANGATTLMFEGVPNYPDPGRFWEVIDKHKVTIFYTAPTAIRALMKEGDEHPKKYDLSSLRLLGSVGEPINPEA 383
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 455 WRWFYNIVGDSRCPISDTWWQTETGGFMITPLPGAWPQKPGSATFPFFGVQPVIVDEKGQEIEGECSGYLCIKKSWPGAF 534
Cdd:PRK00174 384 WEWYYKVVGGERCPIVDTWWQTETGGIMITPLPGATPLKPGSATRPLPGIQPAVVDEEGNPLEGGEGGNLVIKDPWPGMM 463
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 535 RTLYGDHERYETTYFKPFAGYYFTGDGCSRDKDGYHWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAAVVGVEHEV 614
Cdd:PRK00174 464 RTIYGDHERFVKTYFSTFKGMYFTGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVAHPKVAEAAVVGRPDDI 543
|
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 615 KGQGIYAFVTLVDGVPYSEELRKSLIMTVRNQIGAFAAPDKIHWAPGLPKTRSGKIMRRILRKIAARQLdELGDISTLAE 694
Cdd:PRK00174 544 KGQGIYAFVTLKGGEEPSDELRKELRNWVRKEIGPIAKPDVIQFAPGLPKTRSGKIMRRILRKIAEGEE-ILGDTSTLAD 622
|
....*....
gi 242061796 695 PAVVDQLIA 703
Cdd:PRK00174 623 PSVVEKLIE 631
|
|
| Ac_CoA_lig_AcsA |
TIGR02188 |
acetate--CoA ligase; This model describes acetate-CoA ligase (EC 6.2.1.1), also called ... |
67-703 |
0e+00 |
|
acetate--CoA ligase; This model describes acetate-CoA ligase (EC 6.2.1.1), also called acetyl-CoA synthetase and acetyl-activating enzyme. It catalyzes the reaction ATP + acetate + CoA = AMP + diphosphate + acetyl-CoA and belongs to the family of AMP-binding enzymes described by pfam00501.
Pssm-ID: 274022 [Multi-domain] Cd Length: 626 Bit Score: 1073.03 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 67 VSSPKQYREMYERSIKNPAGFWSEIA-ETFYWKEKWNpaEVCSENLdvtkgPVQINWFKGGKTNICYNAVDRNIESgNGD 145
Cdd:TIGR02188 1 IANLEQYKELYEESIEDPDKFWAKLArELLDWFKPFT--KVLDWSF-----PPFYKWFVGGELNVSYNCVDRHLEA-RPD 72
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 146 KIAMYWEGNEPSQDGKLTYSELLEKVCQLANYLKSLGVGKGDAVIIYLPMLLELPIAMLACARIGAVHSVVFAGFSADSL 225
Cdd:TIGR02188 73 KVAIIWEGDEPGEVRKITYRELHREVCRFANVLKSLGVKKGDRVAIYMPMIPEAAIAMLACARIGAIHSVVFGGFSAEAL 152
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 226 AQRIVDCKPKLVITCNAVKRGVKPIFLKDIVDVALvesEKNGVSVGLCLTYenqsamKR---EDTKWQPERDVWWQDVVT 302
Cdd:TIGR02188 153 ADRINDAGAKLVITADEGLRGGKVIPLKAIVDEAL---EKCPVSVEHVLVV------RRtgnPVVPWVEGRDVWWHDLMA 223
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 303 KFPTKCDVEWVDAEDPLFLLYTSGSTGKPKGVLHTSGGYMVYTATTFKYAFDYKPTDIYWCTADCGWITGHSYVTYGPLL 382
Cdd:TIGR02188 224 KASAYCEPEPMDSEDPLFILYTSGSTGKPKGVLHTTGGYLLYAAMTMKYVFDIKDGDIFWCTADVGWITGHSYIVYGPLA 303
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 383 NGATVLVFEGTPNYPDSGRCWDIVDKYNVTIFYTAPTLVRSLMRDGTEYVTRYSRKSLRVLGSVGEPINPSAWRWFYNIV 462
Cdd:TIGR02188 304 NGATTVMFEGVPTYPDPGRFWEIIEKHKVTIFYTAPTAIRALMRLGDEWVKKHDLSSLRLLGSVGEPINPEAWMWYYKVV 383
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 463 GDSRCPISDTWWQTETGGFMITPLPGAWPQKPGSATFPFFGVQPVIVDEKGQEIEG-ECSGYLCIKKSWPGAFRTLYGDH 541
Cdd:TIGR02188 384 GKERCPIVDTWWQTETGGIMITPLPGATPTKPGSATLPFFGIEPAVVDEEGNPVEGpGEGGYLVIKQPWPGMLRTIYGDH 463
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 542 ERYETTYFKPFAGYYFTGDGCSRDKDGYHWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAAVVGVEHEVKGQGIYA 621
Cdd:TIGR02188 464 ERFVDTYFSPFPGYYFTGDGARRDKDGYIWITGRVDDVINVSGHRLGTAEIESALVSHPAVAEAAVVGIPDDIKGQAIYA 543
|
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 622 FVTLVDGVPYSEELRKSLIMTVRNQIGAFAAPDKIHWAPGLPKTRSGKIMRRILRKIAARQLDELGDISTLAEPAVVDQL 701
Cdd:TIGR02188 544 FVTLKDGYEPDDELRKELRKHVRKEIGPIAKPDKIRFVPGLPKTRSGKIMRRLLRKIAAGEAEILGDTSTLEDPSVVEEL 623
|
..
gi 242061796 702 IA 703
Cdd:TIGR02188 624 IE 625
|
|
| Acs |
COG0365 |
Acyl-coenzyme A synthetase/AMP-(fatty) acid ligase [Lipid transport and metabolism]; |
122-703 |
0e+00 |
|
Acyl-coenzyme A synthetase/AMP-(fatty) acid ligase [Lipid transport and metabolism];
Pssm-ID: 440134 [Multi-domain] Cd Length: 565 Bit Score: 883.30 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 122 WFKGGKTNICYNAVDRNIEsGNGDKIAMYWEGnEPSQDGKLTYSELLEKVCQLANYLKSLGVGKGDAVIIYLPMLLELPI 201
Cdd:COG0365 2 WFVGGRLNIAYNCLDRHAE-GRGDKVALIWEG-EDGEERTLTYAELRREVNRFANALRALGVKKGDRVAIYLPNIPEAVI 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 202 AMLACARIGAVHSVVFAGFSADSLAQRIVDCKPKLVITCNAVKRGVKPIFLKDIVDVALVESEkngvSVGLCLTYenqsa 281
Cdd:COG0365 80 AMLACARIGAVHSPVFPGFGAEALADRIEDAEAKVLITADGGLRGGKVIDLKEKVDEALEELP----SLEHVIVV----- 150
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 282 mKREDTKWQPERDVWWQDVVTKFPTKCDVEWVDAEDPLFLLYTSGSTGKPKGVLHTSGGYMVYTATTFKYAFDYKPTDIY 361
Cdd:COG0365 151 -GRTGADVPMEGDLDWDELLAAASAEFEPEPTDADDPLFILYTSGTTGKPKGVVHTHGGYLVHAATTAKYVLDLKPGDVF 229
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 362 WCTADCGWITGHSYVTYGPLLNGATVLVFEGTPNYPDSGRCWDIVDKYNVTIFYTAPTLVRSLMRDGTEYVTRYSRKSLR 441
Cdd:COG0365 230 WCTADIGWATGHSYIVYGPLLNGATVVLYEGRPDFPDPGRLWELIEKYGVTVFFTAPTAIRALMKAGDEPLKKYDLSSLR 309
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 442 VLGSVGEPINPSAWRWFYNIVGdsrCPISDTWWQTETGGFMITPLPGaWPQKPGSATFPFFGVQPVIVDEKGQEIEGECS 521
Cdd:COG0365 310 LLGSAGEPLNPEVWEWWYEAVG---VPIVDGWGQTETGGIFISNLPG-LPVKPGSMGKPVPGYDVAVVDEDGNPVPPGEE 385
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 522 GYLCIKKSWPGAFRTLYGDHERYETTYFKPFAGYYFTGDGCSRDKDGYHWLTGRVDDVINVSGHRIGTAEVESALVSHPQ 601
Cdd:COG0365 386 GELVIKGPWPGMFRGYWNDPERYRETYFGRFPGWYRTGDGARRDEDGYFWILGRSDDVINVSGHRIGTAEIESALVSHPA 465
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 602 CAEAAVVGVEHEVKGQGIYAFVTLVDGVPYSEELRKSLIMTVRNQIGAFAAPDKIHWAPGLPKTRSGKIMRRILRKIAAR 681
Cdd:COG0365 466 VAEAAVVGVPDEIRGQVVKAFVVLKPGVEPSDELAKELQAHVREELGPYAYPREIEFVDELPKTRSGKIMRRLLRKIAEG 545
|
570 580
....*....|....*....|..
gi 242061796 682 QldELGDISTLAEPAVVDQLIA 703
Cdd:COG0365 546 R--PLGDTSTLEDPEALDEIKE 565
|
|
| ACS-like |
cd17634 |
acetate-CoA ligase; This family includes acyl- and aryl-CoA ligases, as well as the ... |
73-671 |
0e+00 |
|
acetate-CoA ligase; This family includes acyl- and aryl-CoA ligases, as well as the adenylation domain of nonribosomal peptide synthetases and firefly luciferases. The adenylate-forming enzymes catalyze an ATP-dependent two-step reaction to first activate a carboxylate substrate as an adenylate and then transfer the carboxylate to the pantetheine group of either coenzyme A or an acyl-carrier protein. The active site of the domain is located at the interface of a large N-terminal subdomain and a smaller C-terminal subdomain.
Pssm-ID: 341289 [Multi-domain] Cd Length: 587 Bit Score: 718.20 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 73 YREMYERSIKNPAGFWSEIAETFYWKekWNPAEVcsENLDVTKGPVQINWFKGGKTNICYNAVDRNIESgNGDKIAMYWE 152
Cdd:cd17634 1 YETKYRQSINDPDTFWGEAGKILDWI--TPYQKV--KNTSFAPGAPSIKWFEDATLNLAANALDRHLRE-NGDRTAIIYE 75
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 153 GNEPSQDGKLTYSELLEKVCQLANYLKSLGVGKGDAVIIYLPMLLELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDC 232
Cdd:cd17634 76 GDDTSQSRTISYRELHREVCRFAGTLLDLGVKKGDRVAIYMPMIPEAAVAMLACARIGAVHSVIFGGFAPEAVAGRIIDS 155
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 233 KPKLVITCNAVKRGVKPIFLKDIVDVALvesEKNGVSVglcltyENQSAMKRE--DTKWQPERDVWWQDVVTKFPTKCDV 310
Cdd:cd17634 156 SSRLLITADGGVRAGRSVPLKKNVDDAL---NPNVTSV------EHVIVLKRTgsDIDWQEGRDLWWRDLIAKASPEHQP 226
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 311 EWVDAEDPLFLLYTSGSTGKPKGVLHTSGGYMVYTATTFKYAFDYKPTDIYWCTADCGWITGHSYVTYGPLLNGATVLVF 390
Cdd:cd17634 227 EAMNAEDPLFILYTSGTTGKPKGVLHTTGGYLVYAATTMKYVFDYGPGDIYWCTADVGWVTGHSYLLYGPLACGATTLLY 306
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 391 EGTPNYPDSGRCWDIVDKYNVTIFYTAPTLVRSLMRDGTEYVTRYSRKSLRVLGSVGEPINPSAWRWFYNIVGDSRCPIS 470
Cdd:cd17634 307 EGVPNWPTPARMWQVVDKHGVNILYTAPTAIRALMAAGDDAIEGTDRSSLRILGSVGEPINPEAYEWYWKKIGKEKCPVV 386
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 471 DTWWQTETGGFMITPLPGAWPQKPGSATFPFFGVQPVIVDEKGQEIEGECSGYLCIKKSWPGAFRTLYGDHERYETTYFK 550
Cdd:cd17634 387 DTWWQTETGGFMITPLPGAIELKAGSATRPVFGVQPAVVDNEGHPQPGGTEGNLVITDPWPGQTRTLFGDHERFEQTYFS 466
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 551 PFAGYYFTGDGCSRDKDGYHWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAAVVGVEHEVKGQGIYAFVTLVDGVP 630
Cdd:cd17634 467 TFKGMYFSGDGARRDEDGYYWITGRSDDVINVAGHRLGTAEIESVLVAHPKVAEAAVVGIPHAIKGQAPYAYVVLNHGVE 546
|
570 580 590 600
....*....|....*....|....*....|....*....|.
gi 242061796 631 YSEELRKSLIMTVRNQIGAFAAPDKIHWAPGLPKTRSGKIM 671
Cdd:cd17634 547 PSPELYAELRNWVRKEIGPLATPDVVHWVDSLPKTRSGKIM 587
|
|
| PrpE |
cd05967 |
Propionyl-CoA synthetase (PrpE); EC 6.2.1.17: propanoate:CoA ligase (AMP-forming) or ... |
73-701 |
0e+00 |
|
Propionyl-CoA synthetase (PrpE); EC 6.2.1.17: propanoate:CoA ligase (AMP-forming) or propionate#CoA ligase (PrpE) catalyzes the first step of the 2-methylcitric acid cycle for propionate catabolism. It activates propionate to propionyl-CoA in a two-step reaction, which proceeds through a propionyl-AMP intermediate and requires ATP and Mg2+. In Salmonella enterica, the PrpE protein is required for growth of Salmonella enterica on propionate and can substitute for the acetyl-CoA synthetase (Acs) enzyme during growth on acetate. PrpE can also activate acetate, 3HP, and butyrate to their corresponding CoA-thioesters, although with less efficiency.
Pssm-ID: 341271 [Multi-domain] Cd Length: 617 Bit Score: 642.83 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 73 YREMYERSIKNPAGFWSEIAETFYWKEKWNPAevcsenLDVTKGPVQiNWFKGGKTNICYNAVDRNIESGNGDKIAMYWE 152
Cdd:cd05967 1 YEEVYARSIAEPEAFWAEQARLIDWFKPPEKI------LDNSNPPFT-RWFVGGRLNTCYNALDRHVEAGRGDQIALIYD 73
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 153 GNEPSQDGKLTYSELLEKVCQLANYLKSLGVGKGDAVIIYLPMLLELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDC 232
Cdd:cd05967 74 SPVTGTERTYTYAELLDEVSRLAGVLRKLGVVKGDRVIIYMPMIPEAAIAMLACARIGAIHSVVFGGFAAKELASRIDDA 153
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 233 KPKLVITCNAVKRGVKPIFLKDIVDVALVESeknGVSVGLCLTYeNQSAMKREDTKwqPERDVWWQDVVTKfPTKCDVEW 312
Cdd:cd05967 154 KPKLIVTASCGIEPGKVVPYKPLLDKALELS---GHKPHHVLVL-NRPQVPADLTK--PGRDLDWSELLAK-AEPVDCVP 226
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 313 VDAEDPLFLLYTSGSTGKPKGVLHTSGGYMVYTATTFKYAFDYKPTDIYWCTADCGWITGHSYVTYGPLLNGATVLVFEG 392
Cdd:cd05967 227 VAATDPLYILYTSGTTGKPKGVVRDNGGHAVALNWSMRNIYGIKPGDVWWAASDVGWVVGHSYIVYGPLLHGATTVLYEG 306
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 393 TP-NYPDSGRCWDIVDKYNVTIFYTAPTLVRSLMRDGT--EYVTRYSRKSLRVLGSVGEPINPSAWRWFYNIVGdsrCPI 469
Cdd:cd05967 307 KPvGTPDPGAFWRVIEKYQVNALFTAPTAIRAIRKEDPdgKYIKKYDLSSLRTLFLAGERLDPPTLEWAENTLG---VPV 383
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 470 SDTWWQTETGGFMITPLPG--AWPQKPGSATFPFFGVQPVIVDEKGQEIEGECSGYLCIKKSW-PGAFRTLYGDHERYET 546
Cdd:cd05967 384 IDHWWQTETGWPITANPVGlePLPIKAGSPGKPVPGYQVQVLDEDGEPVGPNELGNIVIKLPLpPGCLLTLWKNDERFKK 463
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 547 TYFKPFAGYYFTGDGCSRDKDGYHWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAAVVGVEHEVKGQGIYAFVTLV 626
Cdd:cd05967 464 LYLSKFPGYYDTGDAGYKDEDGYLFIMGRTDDVINVAGHRLSTGEMEESVLSHPAVAECAVVGVRDELKGQVPLGLVVLK 543
|
570 580 590 600 610 620 630
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 242061796 627 DGVPYS-EELRKSLIMTVRNQIGAFAAPDKIHWAPGLPKTRSGKIMRRILRKIAarQLDELGDISTLAEPAVVDQL 701
Cdd:cd05967 544 EGVKITaEELEKELVALVREQIGPVAAFRLVIFVKRLPKTRSGKILRRTLRKIA--DGEDYTIPSTIEDPSVLDEI 617
|
|
| prpE |
PRK10524 |
propionyl-CoA synthetase; Provisional |
73-701 |
0e+00 |
|
propionyl-CoA synthetase; Provisional
Pssm-ID: 182517 [Multi-domain] Cd Length: 629 Bit Score: 593.46 E-value: 0e+00
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 73 YREMYERSIKNPAGFWSEIAETFYWKEKwnPAEVCsenlDVTKGPVqINWFKGGKTNICYNAVDRNIESgNGDKIAMYWE 152
Cdd:PRK10524 4 YSEFYQRSIDDPEAFWAEQARRIDWQTP--FTQVL----DYSNPPF-ARWFVGGRTNLCHNAVDRHLAK-RPEQLALIAV 75
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 153 GNEPSQDGKLTYSELLEKVCQLANYLKSLGVGKGDAVIIYLPMLLELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDC 232
Cdd:PRK10524 76 STETDEERTYTFRQLHDEVNRMAAMLRSLGVQRGDRVLIYMPMIAEAAFAMLACARIGAIHSVVFGGFASHSLAARIDDA 155
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 233 KPKLVITCNAVKRGVKPIFLKDIVDVALVESEKNGVSVGLCltyeNQSAMKREdtkWQPERDVWWQDVVTKF-PTKCDVE 311
Cdd:PRK10524 156 KPVLIVSADAGSRGGKVVPYKPLLDEAIALAQHKPRHVLLV----DRGLAPMA---RVAGRDVDYATLRAQHlGARVPVE 228
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 312 WVDAEDPLFLLYTSGSTGKPKGVLHTSGGYMVYTATTFKYAFDYKPTDIYWCTADCGWITGHSYVTYGPLLNGATVLVFE 391
Cdd:PRK10524 229 WLESNEPSYILYTSGTTGKPKGVQRDTGGYAVALATSMDTIFGGKAGETFFCASDIGWVVGHSYIVYAPLLAGMATIMYE 308
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 392 GTPNYPDSGRCWDIVDKYNVTIFYTAPTLVRSLMRDGTEYVTRYSRKSLRVLGSVGEPINPSAWRWFYNIVGdsrCPISD 471
Cdd:PRK10524 309 GLPTRPDAGIWWRIVEKYKVNRMFSAPTAIRVLKKQDPALLRKHDLSSLRALFLAGEPLDEPTASWISEALG---VPVID 385
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 472 TWWQTETGGFMITPLPG--AWPQKPGSATFPFFGVQPVIVDEK-GQEIEGECSGYLCIKKSW-PGAFRTLYGDHERYETT 547
Cdd:PRK10524 386 NYWQTETGWPILAIARGveDRPTRLGSPGVPMYGYNVKLLNEVtGEPCGPNEKGVLVIEGPLpPGCMQTVWGDDDRFVKT 465
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 548 YFKPFAG-YYFTGDGCSRDKDGYHWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAAVVGVEHEVKGQGIYAFVTLV 626
Cdd:PRK10524 466 YWSLFGRqVYSTFDWGIRDADGYYFILGRTDDVINVAGHRLGTREIEESISSHPAVAEVAVVGVKDALKGQVAVAFVVPK 545
|
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 627 DGVPYSEE-----LRKSLIMTVRNQIGAFAAPDKIHWAPGLPKTRSGKIMRRILRKIAARQldELGDISTLAEPAVVDQL 701
Cdd:PRK10524 546 DSDSLADRearlaLEKEIMALVDSQLGAVARPARVWFVSALPKTRSGKLLRRAIQAIAEGR--DPGDLTTIEDPAALQQI 623
|
|
| PRK04319 |
PRK04319 |
acetyl-CoA synthetase; Provisional |
119-692 |
4.56e-179 |
|
acetyl-CoA synthetase; Provisional
Pssm-ID: 235279 [Multi-domain] Cd Length: 570 Bit Score: 522.53 E-value: 4.56e-179
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 119 QINWFKGGKTNICYNAVDRNIESGNGDKIAMYWEGnePSQDGKLTYSELLEKVCQLANYLKSLGVGKGDAVIIYLPMLLE 198
Cdd:PRK04319 33 EFSWLETGKVNIAYEAIDRHADGGRKDKVALRYLD--ASRKEKYTYKELKELSNKFANVLKELGVEKGDRVFIFMPRIPE 110
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 199 LPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPKLVITCNAV-KRGVKPIF--LKDIVDVALVESEKNGvsvglclT 275
Cdd:PRK04319 111 LYFALLGALKNGAIVGPLFEAFMEEAVRDRLEDSEAKVLITTPALlERKPADDLpsLKHVLLVGEDVEEGPG-------T 183
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 276 YENQSAMKREdtkwqperdvwwqdvvtkfPTKCDVEWVDAEDPLFLLYTSGSTGKPKGVLHTSGGyMVYTATTFKYAFDY 355
Cdd:PRK04319 184 LDFNALMEQA-------------------SDEFDIEWTDREDGAILHYTSGSTGKPKGVLHVHNA-MLQHYQTGKYVLDL 243
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 356 KPTDIYWCTADCGWITGHSYVTYGPLLNGATVLVFEGTPnypDSGRCWDIVDKYNVTIFYTAPTLVRSLMRDGTEYVTRY 435
Cdd:PRK04319 244 HEDDVYWCTADPGWVTGTSYGIFAPWLNGATNVIDGGRF---SPERWYRILEDYKVTVWYTAPTAIRMLMGAGDDLVKKY 320
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 436 SRKSLRVLGSVGEPINPSAWRWFYNIVGdsrCPISDTWWQTETGGFMITPLPgAWPQKPGSATFPFFGVQPVIVDEKGQE 515
Cdd:PRK04319 321 DLSSLRHILSVGEPLNPEVVRWGMKVFG---LPIHDNWWMTETGGIMIANYP-AMDIKPGSMGKPLPGIEAAIVDDQGNE 396
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 516 IEGECSGYLCIKKSWPGAFRTLYGDHERYETtYFKPfaGYYFTGDGCSRDKDGYHWLTGRVDDVINVSGHRIGTAEVESA 595
Cdd:PRK04319 397 LPPNRMGNLAIKKGWPSMMRGIWNNPEKYES-YFAG--DWYVSGDSAYMDEDGYFWFQGRVDDVIKTSGERVGPFEVESK 473
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 596 LVSHPQCAEAAVVGVEHEVKGQGIYAFVTLVDGVPYSEELRKSLIMTVRNQIGAFAAPDKIHWAPGLPKTRSGKIMRRIL 675
Cdd:PRK04319 474 LMEHPAVAEAGVIGKPDPVRGEIIKAFVALRPGYEPSEELKEEIRGFVKKGLGAHAAPREIEFKDKLPKTRSGKIMRRVL 553
|
570
....*....|....*...
gi 242061796 676 RkiaARQLD-ELGDISTL 692
Cdd:PRK04319 554 K---AWELGlPEGDLSTM 568
|
|
| AACS_like |
cd05968 |
Uncharacterized acyl-CoA synthetase subfamily similar to Acetoacetyl-CoA synthetase; This ... |
73-695 |
1.25e-169 |
|
Uncharacterized acyl-CoA synthetase subfamily similar to Acetoacetyl-CoA synthetase; This uncharacterized acyl-CoA synthetase family (EC 6.2.1.16, or acetoacetate#CoA ligase or acetoacetate:CoA ligase (AMP-forming)) is highly homologous to acetoacetyl-CoA synthetase. However, the proteins in this family exist in only bacteria and archaea. AACS is a cytosolic ligase that specifically activates acetoacetate to its coenzyme A ester by a two-step reaction. Acetoacetate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is the first step of the mevalonate pathway of isoprenoid biosynthesis via isopentenyl diphosphate. Isoprenoids are a large class of compounds found in all living organisms.
Pssm-ID: 341272 [Multi-domain] Cd Length: 610 Bit Score: 499.71 E-value: 1.25e-169
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 73 YREMYERSIKNPAGFWSEIAETF-YWKekwnpAEVCSENLDVTKGPVQINWFKGGKTNICYNAVDRNIESGNgDKIAMYW 151
Cdd:cd05968 9 LEAFLERSAEDNAWFWGEFVKDVgIEW-----YEPPYQTLDLSGGKPWAAWFVGGRMNIVEQLLDKWLADTR-TRPALRW 82
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 152 EGnEPSQDGKLTYSELLEKVCQLANYLKSLGVGKGDAVIIYLPMLLELPIAMLACARIGAVHSVVFAGFSADSLAQRIVD 231
Cdd:cd05968 83 EG-EDGTSRTLTYGELLYEVKRLANGLRALGVGKGDRVGIYLPMIPEIVPAFLAVARIGGIVVPIFSGFGKEAAATRLQD 161
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 232 CKPKLVITCNAVKRGVKPIFLKDIVDVAlvesekngvsVGLCLTYENQSAMKR--EDTKWQPERDVWWQDVVTKFPTKcd 309
Cdd:cd05968 162 AEAKALITADGFTRRGREVNLKEEADKA----------CAQCPTVEKVVVVRHlgNDFTPAKGRDLSYDEEKETAGDG-- 229
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 310 VEWVDAEDPLFLLYTSGSTGKPKGVLHTSGGYMVYTATTFKYAFDYKPTD-IYWCTaDCGWITGhSYVTYGPLLNGATVL 388
Cdd:cd05968 230 AERTESEDPLMIIYTSGTTGKPKGTVHVHAGFPLKAAQDMYFQFDLKPGDlLTWFT-DLGWMMG-PWLIFGGLILGATMV 307
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 389 VFEGTPNYPDSGRCWDIVDKYNVTIFYTAPTLVRSLMRDGTEYVTRYSRKSLRVLGSVGEPINPSAWRWFYNIVGDSRCP 468
Cdd:cd05968 308 LYDGAPDHPKADRLWRMVEDHEITHLGLSPTLIRALKPRGDAPVNAHDLSSLRVLGSTGEPWNPEPWNWLFETVGKGRNP 387
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 469 ISDTWWQTET-----GGFMITPLpgawpqKPGSATFPFFGVQPVIVDEKGQEIEGEcSGYLCIKKSWPGAFRTLYGDHER 543
Cdd:cd05968 388 IINYSGGTEIsggilGNVLIKPI------KPSSFNGPVPGMKADVLDESGKPARPE-VGELVLLAPWPGMTRGFWRDEDR 460
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 544 YETTYFKPFAGYYFTGDGCSRDKDGYHWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAAVVGVEHEVKGQGIYAFV 623
Cdd:cd05968 461 YLETYWSRFDNVWVHGDFAYYDEEGYFYILGRSDDTINVAGKRVGPAEIESVLNAHPAVLESAAIGVPHPVKGEAIVCFV 540
|
570 580 590 600 610 620 630
....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 242061796 624 TLVDGVPYSEELRKSLIMTVRNQIGAFAAPDKIHWAPGLPKTRSGKIMRRILRkiAARQLDELGDISTLAEP 695
Cdd:cd05968 541 VLKPGVTPTEALAEELMERVADELGKPLSPERILFVKDLPKTRNAKVMRRVIR--AAYLGKELGDLSSLENP 610
|
|
| MACS_like_4 |
cd05969 |
Uncharacterized subfamily of Acetyl-CoA synthetase like family (ACS); This family is most ... |
162-679 |
6.18e-144 |
|
Uncharacterized subfamily of Acetyl-CoA synthetase like family (ACS); This family is most similar to acetyl-CoA synthetase. Acetyl-CoA synthetase (ACS) catalyzes the formation of acetyl-CoA from acetate, CoA, and ATP. Synthesis of acetyl-CoA is carried out in a two-step reaction. In the first step, the enzyme catalyzes the synthesis of acetyl-AMP intermediate from acetate and ATP. In the second step, acetyl-AMP reacts with CoA to produce acetyl-CoA. This enzyme is only present in bacteria.
Pssm-ID: 341273 [Multi-domain] Cd Length: 442 Bit Score: 427.69 E-value: 6.18e-144
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 162 LTYSELLEKVCQLANYLKSLGVGKGDAVIIYLPMLLELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPKLVITCN 241
Cdd:cd05969 1 YTFAQLKVLSARFANVLKSLGVGKGDRVFVLSPRSPELYFSMLGIGKIGAVICPLFSAFGPEAIRDRLENSEAKVLITTE 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 242 AVKRGVKPiflkdivdvalvesekngvsvglcltyenqsamkredtkwqperdvwwqdvvtkfptkcdvewvdaEDPLFL 321
Cdd:cd05969 81 ELYERTDP------------------------------------------------------------------EDPTLL 94
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 322 LYTSGSTGKPKGVLHtSGGYMVYTATTFKYAFDYKPTDIYWCTADCGWITGHSYVTYGPLLNGATVLVFEGTPnypDSGR 401
Cdd:cd05969 95 HYTSGTTGTPKGVLH-VHDAMIFYYFTGKYVLDLHPDDIYWCTADPGWVTGTVYGIWAPWLNGVTNVVYEGRF---DAES 170
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 402 CWDIVDKYNVTIFYTAPTLVRSLMRDGTEYVTRYSRKSLRVLGSVGEPINPSAWRWFYNIVGdsrCPISDTWWQTETGGF 481
Cdd:cd05969 171 WYGIIERVKVTVWYTAPTAIRMLMKEGDELARKYDLSSLRFIHSVGEPLNPEAIRWGMEVFG---VPIHDTWWQTETGSI 247
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 482 MITPLPGAwPQKPGSATFPFFGVQPVIVDEKGQEIEGECSGYLCIKKSWPGAFRTLYGDHERYETtYFKpfAGYYFTGDG 561
Cdd:cd05969 248 MIANYPCM-PIKPGSMGKPLPGVKAAVVDENGNELPPGTKGILALKPGWPSMFRGIWNDEERYKN-SFI--DGWYLTGDL 323
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 562 CSRDKDGYHWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAAVVGVEHEVKGQGIYAFVTLVDGVPYSEELRKSLIM 641
Cdd:cd05969 324 AYRDEDGYFWFVGRADDIIKTSGHRVGPFEVESALMEHPAVAEAGVIGKPDPLRGEIIKAFISLKEGFEPSDELKEEIIN 403
|
490 500 510
....*....|....*....|....*....|....*...
gi 242061796 642 TVRNQIGAFAAPDKIHWAPGLPKTRSGKIMRRILRKIA 679
Cdd:cd05969 404 FVRQKLGAHVAPREIEFVDNLPKTRSGKIMRRVLKAKE 441
|
|
| MACS_like |
cd05972 |
Medium-chain acyl-CoA synthetase (MACS or ACSM); MACS catalyzes the two-step activation of ... |
162-677 |
7.58e-122 |
|
Medium-chain acyl-CoA synthetase (MACS or ACSM); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. The acyl-CoA is a key intermediate in many important biosynthetic and catabolic processes.
Pssm-ID: 341276 [Multi-domain] Cd Length: 428 Bit Score: 370.51 E-value: 7.58e-122
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 162 LTYSELLEKVCQLANYLKSLGVGKGDAVIIYLPMLLELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPKLVITcn 241
Cdd:cd05972 1 WSFRELKRESAKAANVLAKLGLRKGDRVAVLLPRVPELWAVILAVIKLGAVYVPLTTLLGPKDIEYRLEAAGAKAIVT-- 78
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 242 avkrgvkpiflkdivdvalvesekngvsvglcltyenqsamkredtkwqperdvwwqdvvtkfptkcdvewvDAEDPLFL 321
Cdd:cd05972 79 ------------------------------------------------------------------------DAEDPALI 86
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 322 LYTSGSTGKPKGVLHTSGgYMVYTATTFKYAFDYKPTDIYWCTADCGWITGHSYVTYGPLLNGATVLVFEGTPNYPDsgR 401
Cdd:cd05972 87 YFTSGTTGLPKGVLHTHS-YPLGHIPTAAYWLGLRPDDIHWNIADPGWAKGAWSSFFGPWLLGATVFVYEGPRFDAE--R 163
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 402 CWDIVDKYNVTIFYTAPTLVRSLMRDGTEyvtRYSRKSLRVLGSVGEPINPSAWRWFYNIVGdsrCPISDTWWQTETGgF 481
Cdd:cd05972 164 ILELLERYGVTSFCGPPTAYRMLIKQDLS---SYKFSHLRLVVSAGEPLNPEVIEWWRAATG---LPIRDGYGQTETG-L 236
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 482 MITPLPGAwPQKPGSATFPFFGVQPVIVDEKGQEIEGECSGYLCIKKSWPGAFRTLYGDHERYETTYFkpfAGYYFTGDG 561
Cdd:cd05972 237 TVGNFPDM-PVKPGSMGRPTPGYDVAIIDDDGRELPPGEEGDIAIKLPPPGLFLGYVGDPEKTEASIR---GDYYLTGDR 312
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 562 CSRDKDGYHWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAAVVGVEHEVKGQGIYAFVTLVDGVPYSEELRKSLIM 641
Cdd:cd05972 313 AYRDEDGYFWFVGRADDIIKSSGYRIGPFEVESALLEHPAVAEAAVVGSPDPVRGEVVKAFVVLTSGYEPSEELAEELQG 392
|
490 500 510
....*....|....*....|....*....|....*.
gi 242061796 642 TVRNQIGAFAAPDKIHWAPGLPKTRSGKIMRRILRK 677
Cdd:cd05972 393 HVKKVLAPYKYPREIEFVEELPKTISGKIRRVELRD 428
|
|
| AMP-binding |
pfam00501 |
AMP-binding enzyme; |
142-583 |
4.44e-114 |
|
AMP-binding enzyme;
Pssm-ID: 459834 [Multi-domain] Cd Length: 417 Bit Score: 350.07 E-value: 4.44e-114
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 142 GNGDKIAMywegnEPSQDGKLTYSELLEKVCQLANYLKSLGVGKGDAVIIYLPMLLELPIAMLACARIGAVHSVVFAGFS 221
Cdd:pfam00501 7 RTPDKTAL-----EVGEGRRLTYRELDERANRLAAGLRALGVGKGDRVAILLPNSPEWVVAFLACLKAGAVYVPLNPRLP 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 222 ADSLAQRIVDCKPKLVITCNAVKrgvkpiflkdivDVALVESEKNGVSVGLCLTYENQSAMKREDTKWQPERDVWWQdvv 301
Cdd:pfam00501 82 AEELAYILEDSGAKVLITDDALK------------LEELLEALGKLEVVKLVLVLDRDPVLKEEPLPEEAKPADVPP--- 146
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 302 tkfptkCDVEWVDAEDPLFLLYTSGSTGKPKGVLHTSgGYMVYTATTFK----YAFDYKPTDIYWCTADCGWITGHSYVT 377
Cdd:pfam00501 147 ------PPPPPPDPDDLAYIIYTSGTTGKPKGVMLTH-RNLVANVLSIKrvrpRGFGLGPDDRVLSTLPLFHDFGLSLGL 219
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 378 YGPLLNGATVLVFEGTPNyPDSGRCWDIVDKYNVTIFYTAPTLVRSLMRDGTEYVTRYSrkSLRVLGSVGEPINPSAWRW 457
Cdd:pfam00501 220 LGPLLAGATVVLPPGFPA-LDPAALLELIERYKVTVLYGVPTLLNMLLEAGAPKRALLS--SLRLVLSGGAPLPPELARR 296
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 458 FYNIVGdsrCPISDTWWQTETGGFMITPLPGAWPQ-KPGSATFPFFGVQPVIVDEK-GQEIEGECSGYLCIKKswPGAFR 535
Cdd:pfam00501 297 FRELFG---GALVNGYGLTETTGVVTTPLPLDEDLrSLGSVGRPLPGTEVKIVDDEtGEPVPPGEPGELCVRG--PGVMK 371
|
410 420 430 440
....*....|....*....|....*....|....*....|....*...
gi 242061796 536 TLYGDHERYETTYFKPfaGYYFTGDGCSRDKDGYHWLTGRVDDVINVS 583
Cdd:pfam00501 372 GYLNDPELTAEAFDED--GWYRTGDLGRRDEDGYLEIVGRKKDQIKLG 417
|
|
| MenE/FadK |
COG0318 |
O-succinylbenzoic acid-CoA ligase MenE or related acyl-CoA synthetase (AMP-forming) [Lipid ... |
143-685 |
3.93e-94 |
|
O-succinylbenzoic acid-CoA ligase MenE or related acyl-CoA synthetase (AMP-forming) [Lipid transport and metabolism]; O-succinylbenzoic acid-CoA ligase MenE or related acyl-CoA synthetase (AMP-forming) is part of the Pathway/BioSystem: Menaquinone biosynthesis
Pssm-ID: 440087 [Multi-domain] Cd Length: 452 Bit Score: 299.42 E-value: 3.93e-94
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 143 NGDKIAMYWEGNEpsqdgkLTYSELLEKVCQLANYLKSLGVGKGDAVIIYLPMLLELPIAMLACARIGAVHSVVFAGFSA 222
Cdd:COG0318 12 HPDRPALVFGGRR------LTYAELDARARRLAAALRALGVGPGDRVALLLPNSPEFVVAFLAALRAGAVVVPLNPRLTA 85
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 223 DSLAQRIVDCKPKLVITCnavkrgvkpiflkdivdvalvesekngvsvglcltyenqsamkredtkwqperdvwwqdvvt 302
Cdd:COG0318 86 EELAYILEDSGARALVTA-------------------------------------------------------------- 103
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 303 kfptkcdvewvdaedplFLLYTSGSTGKPKGVLHTSGGyMVYTATTFKYAFDYKPTDIYWCTADCGWITGHSYVTYGPLL 382
Cdd:COG0318 104 -----------------LILYTSGTTGRPKGVMLTHRN-LLANAAAIAAALGLTPGDVVLVALPLFHVFGLTVGLLAPLL 165
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 383 NGATVLVFEGtpnyPDSGRCWDIVDKYNVTIFYTAPTLVRSLMRDGTeyVTRYSRKSLRVLGSVGEPINPSAWRWFYNIV 462
Cdd:COG0318 166 AGATLVLLPR----FDPERVLELIERERVTVLFGVPTMLARLLRHPE--FARYDLSSLRLVVSGGAPLPPELLERFEERF 239
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 463 GdsrCPISDTWWQTETGGFMITPLPGAWPQKPGSATFPFFGVQPVIVDEKGQEI----EGEcsgyLCIKKswPGAFRTLY 538
Cdd:COG0318 240 G---VRIVEGYGLTETSPVVTVNPEDPGERRPGSVGRPLPGVEVRIVDEDGRELppgeVGE----IVVRG--PNVMKGYW 310
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 539 GDHEryETTyfKPFA-GYYFTGDGCSRDKDGYHWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAAVVGVEHEVKGQ 617
Cdd:COG0318 311 NDPE--ATA--EAFRdGWLRTGDLGRLDEDGYLYIVGRKKDMIISGGENVYPAEVEEVLAAHPGVAEAAVVGVPDEKWGE 386
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 242061796 618 GIYAFVTLVDGVPYSEElrkSLIMTVRNQIGAFAAPDKIHWAPGLPKTRSGKIMRRILRKIAARQLDE 685
Cdd:COG0318 387 RVVAFVVLRPGAELDAE---ELRAFLRERLARYKVPRRVEFVDELPRTASGKIDRRALRERYAAGALE 451
|
|
| AFD_class_I |
cd04433 |
Adenylate forming domain, Class I, also known as the ANL superfamily; This family is known as ... |
317-671 |
2.44e-87 |
|
Adenylate forming domain, Class I, also known as the ANL superfamily; This family is known as the ANL (acyl-CoA synthetases, the NRPS adenylation domains, and the Luciferase enzymes) superfamily. It includes acyl- and aryl-CoA ligases, as well as the adenylation domain of nonribosomal peptide synthetases and firefly luciferases.The adenylate-forming enzymes catalyze an ATP-dependent two-step reaction to first activate a carboxylate substrate as an adenylate and then transfer the carboxylate to the pantetheine group of either coenzyme A or an acyl-carrier protein. The active site of the domain is located at the interface of a large N-terminal subdomain and a smaller C-terminal subdomain.
Pssm-ID: 341228 [Multi-domain] Cd Length: 336 Bit Score: 277.63 E-value: 2.44e-87
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 317 DPLFLLYTSGSTGKPKGVLHTSGGYMVYTATTFKYaFDYKPTDIYWCTADCGWItGHSYVTYGPLLNGATVLVFEGtpny 396
Cdd:cd04433 1 DPALILYTSGTTGKPKGVVLSHRNLLAAAAALAAS-GGLTEGDVFLSTLPLFHI-GGLFGLLGALLAGGTVVLLPK---- 74
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 397 PDSGRCWDIVDKYNVTIFYTAPTLVRSLMRdgTEYVTRYSRKSLRVLGSVGEPINPSAWRWFYNIVGdsrCPISDTWWQT 476
Cdd:cd04433 75 FDPEAALELIEREKVTILLGVPTLLARLLK--APESAGYDLSSLRALVSGGAPLPPELLERFEEAPG---IKLVNGYGLT 149
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 477 ETGGFMITPLPGAWPQKPGSATFPFFGVQPVIVDEKGQEIEGECSGYLCIKKSWPGAFrtlYGDHEryETTYFKPFAGYY 556
Cdd:cd04433 150 ETGGTVATGPPDDDARKPGSVGRPVPGVEVRIVDPDGGELPPGEIGELVVRGPSVMKG---YWNNP--EATAAVDEDGWY 224
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 557 FTGDGCSRDKDGYHWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAAVVGVEHEVKGQGIYAFVTLVDGVPYSEElr 636
Cdd:cd04433 225 RTGDLGRLDEDGYLYIVGRLKDMIKSGGENVYPAEVEAVLLGHPGVAEAAVVGVPDPEWGERVVAVVVLRPGADLDAE-- 302
|
330 340 350
....*....|....*....|....*....|....*
gi 242061796 637 kSLIMTVRNQIGAFAAPDKIHWAPGLPKTRSGKIM 671
Cdd:cd04433 303 -ELRAHVRERLAPYKVPRRVVFVDALPRTASGKID 336
|
|
| AACS |
cd05943 |
Acetoacetyl-CoA synthetase (acetoacetate-CoA ligase, AACS); AACS is a cytosolic ligase that ... |
73-678 |
4.28e-87 |
|
Acetoacetyl-CoA synthetase (acetoacetate-CoA ligase, AACS); AACS is a cytosolic ligase that specifically activates acetoacetate to its coenzyme A ester by a two-step reaction. Acetoacetate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is the first step of the mevalonate pathway of isoprenoid biosynthesis via isopentenyl diphosphate. Isoprenoids are a large class of compounds found in all living organisms. AACS is widely distributed in bacteria, archaea and eukaryotes. In bacteria, AACS is known to exhibit an important role in the metabolism of poly-b-hydroxybutyrate, an intracellular reserve of organic carbon and chemical energy by some microorganisms. In mammals, AACS influences the rate of ketone body utilization for the formation of physiologically important fatty acids and cholesterol.
Pssm-ID: 341265 [Multi-domain] Cd Length: 629 Bit Score: 286.09 E-value: 4.28e-87
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 73 YREMYERSIKNPAGFWSEIAEtfYWKEKWN-PAEVCSENLDVTKGPVqinWFKGGKTNICYNAVDRnieSGNGDKIAMYW 151
Cdd:cd05943 19 YAALHRWSVDDPGAFWAAVWD--FSGVRGSkPYDVVVVSGRIMPGAR---WFPGARLNYAENLLRH---ADADDPAAIYA 90
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 152 EGNEPSQdgKLTYSELLEKVCQLANYLKSLGVGKGDAVIIYLPMLLELPIAMLACARIGAVHSVVFAGFSADSLAQRIVD 231
Cdd:cd05943 91 AEDGERT--EVTWAELRRRVARLAAALRALGVKPGDRVAGYLPNIPEAVVAMLATASIGAIWSSCSPDFGVPGVLDRFGQ 168
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 232 CKPKLVITCNAVKRGVKPIFLKDIVD--VALVESEKNGVSVGLCLTYENQSAMKREdtkwqpeRDVWWQDVVTKFPT-KC 308
Cdd:cd05943 169 IEPKVLFAVDAYTYNGKRHDVREKVAelVKGLPSLLAVVVVPYTVAAGQPDLSKIA-------KALTLEDFLATGAAgEL 241
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 309 DVEWVDAEDPLFLLYTSGSTGKPKGVLHTSGGYMVYTATTFKYAFDYKPTDIYWCTADCGWITGHSYVTYgpLLNGATVL 388
Cdd:cd05943 242 EFEPLPFDHPLYILYSSGTTGLPKCIVHGAGGTLLQHLKEHILHCDLRPGDRLFYYTTCGWMMWNWLVSG--LAVGATIV 319
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 389 VFEGTPNYPDSGRCWDIVDKYNVTIFYTAPTLVRSLMRDGTEYVTRYSRKSLRVLGSVGEPINPSAWRWFYNIVGdsrcp 468
Cdd:cd05943 320 LYDGSPFYPDTNALWDLADEEGITVFGTSAKYLDALEKAGLKPAETHDLSSLRTILSTGSPLKPESFDYVYDHIK----- 394
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 469 iSDTWWQTETGG------FMIT-PLpgaWPQKPGSATFPFFGVQPVIVDEKGQEIEGEcSGYLCIKKSWPGAFRTLYGDH 541
Cdd:cd05943 395 -PDVLLASISGGtdiiscFVGGnPL---LPVYRGEIQCRGLGMAVEAFDEEGKPVWGE-KGELVCTKPFPSMPVGFWNDP 469
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 542 E--RYETTYFKPFAGYYFTGDGCSRDKDGYHWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAAVVGVEHEVKGQGI 619
Cdd:cd05943 470 DgsRYRAAYFAKYPGVWAHGDWIEITPRGGVVILGRSDGTLNPGGVRIGTAEIYRVVEKIPEVEDSLVVGQEWKDGDERV 549
|
570 580 590 600 610
....*....|....*....|....*....|....*....|....*....|....*....
gi 242061796 620 YAFVTLVDGVPYSEELRKSLIMTVRNQIGAFAAPDKIHWAPGLPKTRSGKIMRRILRKI 678
Cdd:cd05943 550 ILFVKLREGVELDDELRKRIRSTIRSALSPRHVPAKIIAVPDIPRTLSGKKVEVAVKKI 608
|
|
| MACS_like_2 |
cd05973 |
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the ... |
162-677 |
4.17e-84 |
|
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. MACS enzymes are localized to mitochondria.
Pssm-ID: 341277 [Multi-domain] Cd Length: 437 Bit Score: 272.47 E-value: 4.17e-84
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 162 LTYSELLEKVCQLANYLKSLGVGKGDAVIIYLPMLLELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPKLVItCN 241
Cdd:cd05973 1 LTFGELRALSARFANALQELGVGPGDVVAGLLPRTPELVVTILGIWRLGAVYQPLFTAFGPKAIEHRLRTSGARLVV-TD 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 242 AVKRgvkpiflkdivdvalvesekngvsvglcltyenqsamkredtkwqperdvwwqdvvtkfpTKCDvewvdaEDPLFL 321
Cdd:cd05973 80 AANR------------------------------------------------------------HKLD------SDPFVM 93
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 322 LYTSGSTGKPKGVLH------TSGGYMvytattfKYAFDYKPTDIYWCTADCGWITGHSYVTYGPLLNGATVLVFEGtPN 395
Cdd:cd05973 94 MFTSGTTGLPKGVPVplralaAFGAYL-------RDAVDLRPEDSFWNAADPGWAYGLYYAITGPLALGHPTILLEG-GF 165
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 396 YPDSgrCWDIVDKYNVTIFYTAPTLVRSLMRDGTEYVTRySRKSLRVLGSVGEPINPSAWRWFYNIVGdsrCPISDTWWQ 475
Cdd:cd05973 166 SVES--TWRVIERLGVTNLAGSPTAYRLLMAAGAEVPAR-PKGRLRRVSSAGEPLTPEVIRWFDAALG---VPIHDHYGQ 239
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 476 TETGGFMITPLPGAWPQKPGSATFPFFGVQPVIVDEKGQEIEGECSGYLCIKKS-----WpgaFRTLYGDHERyettyfK 550
Cdd:cd05973 240 TELGMVLANHHALEHPVHAGSAGRAMPGWRVAVLDDDGDELGPGEPGRLAIDIAnsplmW---FRGYQLPDTP------A 310
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 551 PFAGYYFTGDGCSRDKDGYHWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAAVVGVEHEVKGQGIYAFVTLVDGVP 630
Cdd:cd05973 311 IDGGYYLTGDTVEFDPDGSFSFIGRADDVITMSGYRIGPFDVESALIEHPAVAEAAVIGVPDPERTEVVKAFVVLRGGHE 390
|
490 500 510 520
....*....|....*....|....*....|....*....|....*..
gi 242061796 631 YSEELRKSLIMTVRNQIGAFAAPDKIHWAPGLPKTRSGKIMRRILRK 677
Cdd:cd05973 391 GTPALADELQLHVKKRLSAHAYPRTIHFVDELPKTPSGKIQRFLLRR 437
|
|
| PTZ00237 |
PTZ00237 |
acetyl-CoA synthetase; Provisional |
68-677 |
3.77e-81 |
|
acetyl-CoA synthetase; Provisional
Pssm-ID: 240325 [Multi-domain] Cd Length: 647 Bit Score: 270.84 E-value: 3.77e-81
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 68 SSPKQYREMYERSIKNPAGFWSEIAETF-YWKEKWNpaEVCSENldvtkgPVQINWFKGGKTNICYNAVDRNIESG-NGD 145
Cdd:PTZ00237 5 SDPFDYENDSNYANSNPESFWDEVAKKYvHWDKMYD--KVYSGD------EIYPDWFKGGELNTCYNVLDIHVKNPlKRD 76
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 146 KIAMYWEGNEPSQDGKLTYSELLEKVCQLANYLKSLGVGKGDAVIIYLPMLLELPIAMLACARIGAVHSVVFAGFSADSL 225
Cdd:PTZ00237 77 QDALIYECPYLKKTIKLTYYQLYEKVCEFSRVLLNLNISKNDNVLIYMANTLEPLIAMLSCARIGATHCVLFDGYSVKSL 156
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 226 AQRIVDCKPKLVITCNAvkrgvkPIFLKDIVD--------VALVESEKNGVSVGLCLTYENQSAMKR-EDTKWQPErDVW 296
Cdd:PTZ00237 157 IDRIETITPKLIITTNY------GILNDEIITftpnlkeaIELSTFKPSNVITLFRNDITSESDLKKiETIPTIPN-TLS 229
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 297 WQDVVTKFP---TKCDVEWVDAED--PLFLLYTSGSTGKPKGVLHTSGGYMVYTATTFKYAFDYKPTDIYWCTADCGWIT 371
Cdd:PTZ00237 230 WYDEIKKIKennQSPFYEYVPVESshPLYILYTSGTTGNSKAVVRSNGPHLVGLKYYWRSIIEKDIPTVVFSHSSIGWVS 309
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 372 GHSYVtYGPLLNGATVLVFEG--TPNYPDSGRCWDIVDKYNVTIFYTAPTLVRSLMR---DGTEYVTRYSRKSLRVLGSV 446
Cdd:PTZ00237 310 FHGFL-YGSLSLGNTFVMFEGgiIKNKHIEDDLWNTIEKHKVTHTLTLPKTIRYLIKtdpEATIIRSKYDLSNLKEIWCG 388
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 447 GEPINPSAWRWFYNIVgdsRCPISDTWWQTETGgfmITPLPG-AWPQKPGSAT-FPFFGVQPVIVDEKGQEIEGECSGYL 524
Cdd:PTZ00237 389 GEVIEESIPEYIENKL---KIKSSRGYGQTEIG---ITYLYCyGHINIPYNATgVPSIFIKPSILSEDGKELNVNEIGEV 462
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 525 CIKKSWPGAF-RTLYGDHERYETTYFKpFAGYYFTGDGCSRDKDGYHWLTGRVDDVINVSGHRIGTAEVESALVSHPQCA 603
Cdd:PTZ00237 463 AFKLPMPPSFaTTFYKNDEKFKQLFSK-FPGYYNSGDLGFKDENGYYTIVSRSDDQIKISGNKVQLNTIETSILKHPLVL 541
|
570 580 590 600 610 620 630
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 242061796 604 EAAVVGVEHEVKGQGIYAFVTLVDGVPYSE----ELRKSLIMTVRNQIGAFAAPDKIHWAPGLPKTRSGKIMRRILRK 677
Cdd:PTZ00237 542 ECCSIGIYDPDCYNVPIGLLVLKQDQSNQSidlnKLKNEINNIITQDIESLAVLRKIIIVNQLPKTKTGKIPRQIISK 619
|
|
| PRK03584 |
PRK03584 |
acetoacetate--CoA ligase; |
73-705 |
2.61e-77 |
|
acetoacetate--CoA ligase;
Pssm-ID: 235134 [Multi-domain] Cd Length: 655 Bit Score: 260.88 E-value: 2.61e-77
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 73 YREMYERSIKNPAGFWSEIAEtfYWKEKWNPAEVCSENLDVTKGPVqinWFKGGKTNICYNAVdRNiesGNGDKIAMYWE 152
Cdd:PRK03584 36 YAALWRWSVEDLEAFWQSVWD--FFGVIGSTPYTVVLAGRRMPGAR---WFPGARLNYAENLL-RH---RRDDRPAIIFR 106
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 153 GnEPSQDGKLTYSELLEKVCQLANYLKSLGVGKGDAVIIYLPMLLELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDC 232
Cdd:PRK03584 107 G-EDGPRRELSWAELRRQVAALAAALRALGVGPGDRVAAYLPNIPETVVAMLATASLGAIWSSCSPDFGVQGVLDRFGQI 185
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 233 KPKLVITCNAVKRGVKPIflkDIVDV--ALVE---SEKNGVSVglclTYENQSAMKREDTKWQPerdvwWQDVVTKFPTK 307
Cdd:PRK03584 186 EPKVLIAVDGYRYGGKAF---DRRAKvaELRAalpSLEHVVVV----PYLGPAAAAAALPGALL-----WEDFLAPAEAA 253
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 308 -CDVEWVDAEDPLFLLYTSGSTGKPKGVLHTSGGYMVYTATTFKYAFDYKPTD-IYWCTAdCGWITGHSYVtyGPLLNGA 385
Cdd:PRK03584 254 eLEFEPVPFDHPLWILYSSGTTGLPKCIVHGHGGILLEHLKELGLHCDLGPGDrFFWYTT-CGWMMWNWLV--SGLLVGA 330
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 386 TVLVFEGTPNYPDSGRCWDIVDKYNVTIFYTAPTLVRSLMRDGTEYVTRYSRKSLRVLGSVGEPINPSAWRWFYNIVGds 465
Cdd:PRK03584 331 TLVLYDGSPFYPDPNVLWDLAAEEGVTVFGTSAKYLDACEKAGLVPGETHDLSALRTIGSTGSPLPPEGFDWVYEHVK-- 408
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 466 rcpiSDTWWQTETGGfmiTPLPGAW-------PQKPGSATFPFFGVQPVIVDEKGQEIEGEcSGYLCIKKSWPGafRTLY 538
Cdd:PRK03584 409 ----ADVWLASISGG---TDICSCFvggnpllPVYRGEIQCRGLGMAVEAWDEDGRPVVGE-VGELVCTKPFPS--MPLG 478
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 539 ----GDHERYETTYFKPFAGYYFTGDGCSRDKDGYHWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAAVVGVEHEV 614
Cdd:PRK03584 479 fwndPDGSRYRDAYFDTFPGVWRHGDWIEITEHGGVVIYGRSDATLNRGGVRIGTAEIYRQVEALPEVLDSLVIGQEWPD 558
|
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 615 KGQGIYAFVTLVDGVPYSEELRKSLIMTVRNQIGAFAAPDKIHWAPGLPKTRSGKIM----RRILRKiaaRQLDELGDIS 690
Cdd:PRK03584 559 GDVRMPLFVVLAEGVTLDDALRARIRTTIRTNLSPRHVPDKIIAVPDIPRTLSGKKVelpvKKLLHG---RPVKKAVNRD 635
|
650
....*....|....*
gi 242061796 691 TLAEPAVVDQLIALS 705
Cdd:PRK03584 636 ALANPEALDWFADLA 650
|
|
| MACS_like_3 |
cd05971 |
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the ... |
161-676 |
3.59e-76 |
|
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. MACS enzymes are localized to mitochondria.
Pssm-ID: 341275 [Multi-domain] Cd Length: 439 Bit Score: 251.58 E-value: 3.59e-76
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 161 KLTYSELLEKVCQLANYLKSLGVGKGDAVIIYLPMLLELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPKLVITc 240
Cdd:cd05971 6 KVTFKELKTASNRFANVLKEIGLEKGDRVGVFLSQGPECAIAHIAILRSGAIAVPLFALFGPEALEYRLSNSGASALVT- 84
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 241 navkrgvkpiflkDIVDvalvesekngvsvglcltyenqsamkredtkwqperdvwwqdvvtkfptkcdvewvdaeDPLF 320
Cdd:cd05971 85 -------------DGSD-----------------------------------------------------------DPAL 92
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 321 LLYTSGSTGKPKGVLHtsgGYMVYT--ATTFKYAFDYKPT--DIYWCTADCGWITGHSYVTYGPLLNGATVLVFEGTPNy 396
Cdd:cd05971 93 IIYTSGTTGPPKGALH---AHRVLLghLPGVQFPFNLFPRdgDLYWTPADWAWIGGLLDVLLPSLYFGVPVLAHRMTKF- 168
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 397 pDSGRCWDIVDKYNVTIFYTAPTLVRsLMRDGTEYVTRYSRKsLRVLGSVGEPINPSAWRWFYNIVGDsrcPISDTWWQT 476
Cdd:cd05971 169 -DPKAALDLMSRYGVTTAFLPPTALK-MMRQQGEQLKHAQVK-LRAIATGGESLGEELLGWAREQFGV---EVNEFYGQT 242
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 477 EtGGFMITPLPGAWPQKPGSATFPFFGVQPVIVDEKGQEIEGECSGYLCIKKSWPGAFRTLYGDHERYEttyfKPFAGYY 556
Cdd:cd05971 243 E-CNLVIGNCSALFPIKPGSMGKPIPGHRVAIVDDNGTPLPPGEVGEIAVELPDPVAFLGYWNNPSATE----KKMAGDW 317
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 557 F-TGDGCSRDKDGYHWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAAVVGVEHEVKGQGIYAFVTLVDGVPYSEEL 635
Cdd:cd05971 318 LlTGDLGRKDSDGYFWYVGRDDDVITSSGYRIGPAEIEECLLKHPAVLMAAVVGIPDPIRGEIVKAFVVLNPGETPSDAL 397
|
490 500 510 520
....*....|....*....|....*....|....*....|.
gi 242061796 636 RKSLIMTVRNQIGAFAAPDKIHWAPGLPKTRSGKIMRRILR 676
Cdd:cd05971 398 AREIQELVKTRLAAHEYPREIEFVNELPRTATGKIRRRELR 438
|
|
| MACS_AAE_MA_like |
cd05970 |
Medium-chain acyl-CoA synthetase (MACS) of AAE_MA like; MACS catalyzes the two-step activation ... |
129-676 |
1.30e-73 |
|
Medium-chain acyl-CoA synthetase (MACS) of AAE_MA like; MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This family of MACS enzymes is found in archaea and bacteria. It is represented by the acyl-adenylating enzyme from Methanosarcina acetivorans (AAE_MA). AAE_MA is most active with propionate, butyrate, and the branched analogs: 2-methyl-propionate, butyrate, and pentanoate. The specific activity is weaker for smaller or larger acids.
Pssm-ID: 341274 [Multi-domain] Cd Length: 537 Bit Score: 247.79 E-value: 1.30e-73
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 129 NICYNAVDRNIESgNGDKIAMYWeGNEPSQDGKLTYSELLEKVCQLANYLKSLGVGKGDAVIIYLPMLLELPIAMLACAR 208
Cdd:cd05970 17 NFAYDVVDAMAKE-YPDKLALVW-CDDAGEERIFTFAELADYSDKTANFFKAMGIGKGDTVMLTLKRRYEFWYSLLALHK 94
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 209 IGAVHSVVFAGFSADSLAQRIVDCKPKLVItcnAVKRGVKPiflkDIVDVALVE--SEKNGVSVGlcltyenqsamkred 286
Cdd:cd05970 95 LGAIAIPATHQLTAKDIVYRIESADIKMIV---AIAEDNIP----EEIEKAAPEcpSKPKLVWVG--------------- 152
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 287 tkwQPERDVW------WQDVVTKFPTKCDVEWVDAEDPLFLLYTSGSTGKPKGVLHTSGgYMVYTATTFKYAFDYKPTDI 360
Cdd:cd05970 153 ---DPVPEGWidfrklIKNASPDFERPTANSYPCGEDILLVYFSSGTTGMPKMVEHDFT-YPLGHIVTAKYWQNVREGGL 228
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 361 YWCTADCGWITGHSYVTYGPLLNGATVLVFEGTPNYPDSgrCWDIVDKYNVTIFYTAPTLVRSLMRdgtEYVTRYSRKSL 440
Cdd:cd05970 229 HLTVADTGWGKAVWGKIYGQWIAGAAVFVYDYDKFDPKA--LLEKLSKYGVTTFCAPPTIYRFLIR---EDLSRYDLSSL 303
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 441 RVLGSVGEPINPSAWRWFYNIVGDSrcpISDTWWQTETGgFMITPLPGAWPqKPGSATFPFFGVQPVIVDEKGQEIEGEC 520
Cdd:cd05970 304 RYCTTAGEALNPEVFNTFKEKTGIK---LMEGFGQTETT-LTIATFPWMEP-KPGSMGKPAPGYEIDLIDREGRSCEAGE 378
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 521 SGYLCIK--KSWP-GAFRTLYGDHERYETTYFKpfaGYYFTGDGCSRDKDGYHWLTGRVDDVINVSGHRIGTAEVESALV 597
Cdd:cd05970 379 EGEIVIRtsKGKPvGLFGGYYKDAEKTAEVWHD---GYYHTGDAAWMDEDGYLWFVGRTDDLIKSSGYRIGPFEVESALI 455
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 242061796 598 SHPQCAEAAVVGVEHEVKGQGIYAFVTLVDGVPYSEELRKSLIMTVRNQIGAFAAPDKIHWAPGLPKTRSGKIMRRILR 676
Cdd:cd05970 456 QHPAVLECAVTGVPDPIRGQVVKATIVLAKGYEPSEELKKELQDHVKKVTAPYKYPRIVEFVDELPKTISGKIRRVEIR 534
|
|
| BCL_4HBCL |
cd05959 |
Benzoate CoA ligase (BCL) and 4-Hydroxybenzoate-Coenzyme A Ligase (4-HBA-CoA ligase); Benzoate ... |
132-676 |
1.19e-69 |
|
Benzoate CoA ligase (BCL) and 4-Hydroxybenzoate-Coenzyme A Ligase (4-HBA-CoA ligase); Benzoate CoA ligase and 4-hydroxybenzoate-coenzyme A ligase catalyze the first activating step for benzoate and 4-hydroxybenzoate catabolic pathways, respectively. Although these two enzymes share very high sequence homology, they have their own substrate preference. The reaction proceeds via a two-step process; the first ATP-dependent step forms the substrate-AMP intermediate, while the second step forms the acyl-CoA ester, releasing the AMP. Aromatic compounds represent the second most abundant class of organic carbon compounds after carbohydrates. Some bacteria can use benzoic acid or benzenoid compounds as the sole source of carbon and energy through degradation. Benzoate CoA ligase and 4-hydroxybenzoate-Coenzyme A ligase are key enzymes of this process.
Pssm-ID: 341269 [Multi-domain] Cd Length: 508 Bit Score: 236.50 E-value: 1.19e-69
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 132 YNA---VDRNIESGNGDKIAMYwegnepSQDGKLTYSELLEKVCQLANYLKSLGVGKGDAVIIYLPMLLELPIAMLACAR 208
Cdd:cd05959 3 YNAatlVDLNLNEGRGDKTAFI------DDAGSLTYAELEAEARRVAGALRALGVKREERVLLIMLDTVDFPTAFLGAIR 76
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 209 IGAVHSVVFAGFSADSLAQRIVDCKPKLVItcnavkrgVKPIFLkdivdvALVESEKNGVSVGLCLTYENQSAMKREDTK 288
Cdd:cd05959 77 AGIVPVPVNTLLTPDDYAYYLEDSRARVVV--------VSGELA------PVLAAALTKSEHTLVVLIVSGGAGPEAGAL 142
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 289 WQPERDVWWQDVVTKFPTKCDvewvdaeDPLFLLYTSGSTGKPKGVLHTSGGyMVYTATTF-KYAFDYKPTDIYWCTADC 367
Cdd:cd05959 143 LLAELVAAEAEQLKPAATHAD-------DPAFWLYSSGSTGRPKGVVHLHAD-IYWTAELYaRNVLGIREDDVCFSAAKL 214
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 368 GWITGHSYVTYGPLLNGATVLVFegtPNYPDSGRCWDIVDKYNVTIFYTAPTLVRSLMRDgtEYVTRYSRKSLRVLGSVG 447
Cdd:cd05959 215 FFAYGLGNSLTFPLSVGATTVLM---PERPTPAAVFKRIRRYRPTVFFGVPTLYAAMLAA--PNLPSRDLSSLRLCVSAG 289
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 448 EPINPSAWRWFYNIVGdsrCPISDTWWQTETGGFMITPLPGAwpQKPGSATFPFFGVQPVIVDEKGQEIEGECSGYLCIK 527
Cdd:cd05959 290 EALPAEVGERWKARFG---LDILDGIGSTEMLHIFLSNRPGR--VRYGTTGKPVPGYEVELRDEDGGDVADGEPGELYVR 364
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 528 KswpGAFRTLYGDheRYETTYfKPFAGYYF-TGDGCSRDKDGYHWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAA 606
Cdd:cd05959 365 G---PSSATMYWN--NRDKTR-DTFQGEWTrTGDKYVRDDDGFYTYAGRADDMLKVSGIWVSPFEVESALVQHPAVLEAA 438
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 607 VVGVEHEVKGQGIYAFVTLVDGVPYSEELRKSLIMTVRNQIGAFAAPDKIHWAPGLPKTRSGKIMRRILR 676
Cdd:cd05959 439 VVGVEDEDGLTKPKAFVVLRPGYEDSEALEEELKEFVKDRLAPYKYPRWIVFVDELPKTATGKIQRFKLR 508
|
|
| BCL_like |
cd05919 |
Benzoate CoA ligase (BCL) and similar adenylate forming enzymes; This family contains benzoate ... |
159-676 |
4.66e-63 |
|
Benzoate CoA ligase (BCL) and similar adenylate forming enzymes; This family contains benzoate CoA ligase (BCL) and related ligases that catalyze the acylation of benzoate derivatives, 2-aminobenzoate and 4-hydroxybenzoate. Aromatic compounds represent the second most abundant class of organic carbon compounds after carbohydrates. Xenobiotic aromatic compounds are also a major class of man-made pollutants. Some bacteria use benzoate as the sole source of carbon and energy through benzoate degradation. Benzoate degradation starts with its activation to benzoyl-CoA by benzoate CoA ligase. The reaction catalyzed by benzoate CoA ligase proceeds via a two-step process; the first ATP-dependent step forms an acyl-AMP intermediate, and the second step forms the acyl-CoA ester with release of the AMP.
Pssm-ID: 341243 [Multi-domain] Cd Length: 436 Bit Score: 216.56 E-value: 4.66e-63
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 159 DGKLTYSELLEKVCQLANYLKSLGVGKGDAVIIYLPMLLELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPKLVI 238
Cdd:cd05919 8 DRSVTYGQLHDGANRLGSALRNLGVSSGDRVLLLMLDSPELVQLFLGCLARGAIAVVINPLLHPDDYAYIARDCEARLVV 87
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 239 TcnavkrgvkpiflkdivdvalvesekngvsvglcltyenqsamkredtkwqperdvwwqdvvtkfptkcdvewvDAEDP 318
Cdd:cd05919 88 T--------------------------------------------------------------------------SADDI 93
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 319 LFLLYTSGSTGKPKGVLHTSGGYMVYTATTFKYAFDYKPTDIYWCTADC--GWITGHSyvTYGPLLNGATVLVFegtPNY 396
Cdd:cd05919 94 AYLLYSSGTTGPPKGVMHAHRDPLLFADAMAREALGLTPGDRVFSSAKMffGYGLGNS--LWFPLAVGASAVLN---PGW 168
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 397 PDSGRCWDIVDKYNVTIFYTAPTLVRSLMRDGTeyVTRYSRKSLRVLGSVGEPINPSAWRWFYNIVGdsrCPISDTWWQT 476
Cdd:cd05919 169 PTAERVLATLARFRPTVLYGVPTFYANLLDSCA--GSPDALRSLRLCVSAGEALPRGLGERWMEHFG---GPILDGIGAT 243
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 477 ETGGFMITPLPGAWpqKPGSATFPFFGVQPVIVDEKGQEIEGECSGYLCIKKswPGAFRTLYGDHERYETTYFkpfAGYY 556
Cdd:cd05919 244 EVGHIFLSNRPGAW--RLGSTGRPVPGYEIRLVDEEGHTIPPGEEGDLLVRG--PSAAVGYWNNPEKSRATFN---GGWY 316
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 557 FTGDGCSRDKDGYHWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAAVVGVEHEVKGQGIYAFVTLVDGVPYSEELR 636
Cdd:cd05919 317 RTGDKFCRDADGWYTHAGRADDMLKVGGQWVSPVEVESLIIQHPAVAEAAVVAVPESTGLSRLTAFVVLKSPAAPQESLA 396
|
490 500 510 520
....*....|....*....|....*....|....*....|
gi 242061796 637 KSLIMTVRNQIGAFAAPDKIHWAPGLPKTRSGKIMRRILR 676
Cdd:cd05919 397 RDIHRHLLERLSAHKVPRRIAFVDELPRTATGKLQRFKLR 436
|
|
| PRK06187 |
PRK06187 |
long-chain-fatty-acid--CoA ligase; Validated |
143-676 |
1.87e-61 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 235730 [Multi-domain] Cd Length: 521 Bit Score: 214.67 E-value: 1.87e-61
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 143 NGDKIAMYWEGnepsqdGKLTYSELLEKVCQLANYLKSLGVGKGDAVIIYL---PMLLElpiAMLACARIGAV-HSV-VF 217
Cdd:PRK06187 19 HPDKEAVYFDG------RRTTYAELDERVNRLANALRALGVKKGDRVAVFDwnsHEYLE---AYFAVPKIGAVlHPInIR 89
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 218 agFSADSLAQRIVDCKPKLVItcnaVKRGVKPIF------LKDIVDVALVESEKNGVSVGLCLTYENQSAMkredtkwQP 291
Cdd:PRK06187 90 --LKPEEIAYILNDAEDRVVL----VDSEFVPLLaailpqLPTVRTVIVEGDGPAAPLAPEVGEYEELLAA-------AS 156
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 292 ERDVWWQdvvtkfptkcdvewVDAEDPLFLLYTSGSTGKPKGV--------LHTSGGymvytattfKYAFDYKPTDIY-- 361
Cdd:PRK06187 157 DTFDFPD--------------IDENDAAAMLYTSGTTGHPKGVvlshrnlfLHSLAV---------CAWLKLSRDDVYlv 213
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 362 ---------WctadcGWitghsyvTYGPLLNGATVLvfegtpnYPDS---GRCWDIVDKYNVTIFYTAPTLVRSLMRDGT 429
Cdd:PRK06187 214 ivpmfhvhaW-----GL-------PYLALMAGAKQV-------IPRRfdpENLLDLIETERVTFFFAVPTIWQMLLKAPR 274
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 430 EYVTRYSrkSLRVLGSVGEPINPSAWRWFYNIVGdsrCPISDTWWQTETGGFM-ITPL----PGAWPqKPGSATFPFFGV 504
Cdd:PRK06187 275 AYFVDFS--SLRLVIYGGAALPPALLREFKEKFG---IDLVQGYGMTETSPVVsVLPPedqlPGQWT-KRRSAGRPLPGV 348
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 505 QPVIVDEKGQEIE--GECSGYLCIKKSWpgafRTL--YGDHERYETTYFKpfaGYYFTGDGCSRDKDGYHWLTGRVDDVI 580
Cdd:PRK06187 349 EARIVDDDGDELPpdGGEVGEIIVRGPW----LMQgyWNRPEATAETIDG---GWLHTGDVGYIDEDGYLYITDRIKDVI 421
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 581 NVSGHRIGTAEVESALVSHPQCAEAAVVGVEHEVKGQGIYAFVTLVDGVPYSE-ELRKSLimtvRNQIGAFAAPDKIHWA 659
Cdd:PRK06187 422 ISGGENIYPRELEDALYGHPAVAEVAVIGVPDEKWGERPVAVVVLKPGATLDAkELRAFL----RGRLAKFKLPKRIAFV 497
|
570
....*....|....*..
gi 242061796 660 PGLPKTRSGKIMRRILR 676
Cdd:PRK06187 498 DELPRTSVGKILKRVLR 514
|
|
| MACS_euk |
cd05928 |
Eukaryotic Medium-chain acyl-CoA synthetase (MACS or ACSM); MACS catalyzes the two-step ... |
150-677 |
1.41e-60 |
|
Eukaryotic Medium-chain acyl-CoA synthetase (MACS or ACSM); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. The acyl-CoA is a key intermediate in many important biosynthetic and catabolic processes. MACS enzymes are localized to mitochondria. Two murine MACS family proteins are found in liver and kidney. In rodents, a MACS member is detected particularly in the olfactory epithelium and is called O-MACS. O-MACS demonstrates substrate preference for the fatty acid lengths of C6-C12.
Pssm-ID: 341251 [Multi-domain] Cd Length: 530 Bit Score: 212.33 E-value: 1.41e-60
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 150 YWEGNEPSQDGKLTYSELLEKVCQLANYL-KSLGVGKGDAVIIYLPMLLELPIAMLACARIGAVHSVVFAGFSADSLAQR 228
Cdd:cd05928 30 LWWVNGKGDEVKWSFRELGSLSRKAANVLsGACGLQRGDRVAVILPRVPEWWLVNVACIRTGLVFIPGTIQLTAKDILYR 109
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 229 IVDCKPKLVITCNAVKRGVKPIFLK-DIVDVALVESEKNgvsvglcltyenqsamkredtkwqpeRDVW--WQDVVTKFP 305
Cdd:cd05928 110 LQASKAKCIVTSDELAPEVDSVASEcPSLKTKLLVSEKS--------------------------RDGWlnFKELLNEAS 163
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 306 TKCDVEWVDAEDPLFLLYTSGSTGKPKGVLHTSGGYMVYTATTFKYAFDYKPTDIYWCTADCGWITGHSYVTYGPLLNGA 385
Cdd:cd05928 164 TEHHCVETGSQEPMAIYFTSGTTGSPKMAEHSHSSLGLGLKVNGRYWLDLTASDIMWNTSDTGWIKSAWSSLFEPWIQGA 243
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 386 TVLVFEgTPNYpDSGRCWDIVDKYNVTIFYTAPTLVRSLMRDGteyVTRYSRKSLRVLGSVGEPINPSAWRWFYNIVGds 465
Cdd:cd05928 244 CVFVHH-LPRF-DPLVILKTLSSYPITTFCGAPTVYRMLVQQD---LSSYKFPSLQHCVTGGEPLNPEVLEKWKAQTG-- 316
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 466 rCPISDTWWQTETGgfMITPLPGAWPQKPGS--ATFPFFGVQpvIVDEKGQEI----EGECSgyLCIKKSWPGAFRTLY- 538
Cdd:cd05928 317 -LDIYEGYGQTETG--LICANFKGMKIKPGSmgKASPPYDVQ--IIDDNGNVLppgtEGDIG--IRVKPIRPFGLFSGYv 389
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 539 GDHERYETTYFKPFagyYFTGDGCSRDKDGYHWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAAVVGVEHEVKGQG 618
Cdd:cd05928 390 DNPEKTAATIRGDF---YLTGDRGIMDEDGYFWFMGRADDVINSSGYRIGPFEVESALIEHPAVVESAVVSSPDPIRGEV 466
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|...
gi 242061796 619 IYAFVTLVdgVPYS----EELRKSLIMTVRNQIGAFAAPDKIHWAPGLPKTRSGKIMRRILRK 677
Cdd:cd05928 467 VKAFVVLA--PQFLshdpEQLTKELQQHVKSVTAPYKYPRKVEFVQELPKTVTGKIQRNELRD 527
|
|
| FC-FACS_FadD_like |
cd05936 |
Prokaryotic long-chain fatty acid CoA synthetases similar to Escherichia coli FadD; This ... |
143-676 |
2.87e-60 |
|
Prokaryotic long-chain fatty acid CoA synthetases similar to Escherichia coli FadD; This subfamily of the AMP-forming adenylation family contains Escherichia coli FadD and similar prokaryotic fatty acid CoA synthetases. FadD was characterized as a long-chain fatty acid CoA synthetase. The gene fadD is regulated by the fatty acid regulatory protein FadR. Fatty acid CoA synthetase catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, followed by the formation of a fatty acyl-CoA. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341259 [Multi-domain] Cd Length: 468 Bit Score: 209.73 E-value: 2.87e-60
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 143 NGDKIAMYWegnepsQDGKLTYSELLEKVCQLANYLKSLGVGKGDAVIIYLPMLLELPIAMLACARIGAVhsVVFAG--F 220
Cdd:cd05936 12 FPDKTALIF------MGRKLTYRELDALAEAFAAGLQNLGVQPGDRVALMLPNCPQFPIAYFGALKAGAV--VVPLNplY 83
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 221 SADSLAQRIVDCKPKLVITcnavkrgvkpiflkdivdvalvesekngvsvglcltyenqsamkredtkwqperDVWWQDV 300
Cdd:cd05936 84 TPRELEHILNDSGAKALIV------------------------------------------------------AVSFTDL 109
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 301 VTKFPTKCDVEWVDAEDPLFLLYTSGSTGKPKGVLHTSGGyMVYTATTFK--YAFDYKPTDIYWCTADCgwitghsYVTY 378
Cdd:cd05936 110 LAAGAPLGERVALTPEDVAVLQYTSGTTGVPKGAMLTHRN-LVANALQIKawLEDLLEGDDVVLAALPL-------FHVF 181
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 379 G-------PLLNGATVLVFegtPNyPDSGRCWDIVDKYNVTIFYTAPTLVRSLMrdGTEYVTRYSRKSLRVLGSVGEPIN 451
Cdd:cd05936 182 GltvalllPLALGATIVLI---PR-FRPIGVLKEIRKHRVTIFPGVPTMYIALL--NAPEFKKRDFSSLRLCISGGAPLP 255
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 452 PSAWRWFYNIVGdsrCPISDTWWQTETGgfmitPL----PGAWPQKPGSATFPFFGVQPVIVDEKGQEIE-GEcSGYLCI 526
Cdd:cd05936 256 VEVAERFEELTG---VPIVEGYGLTETS-----PVvavnPLDGPRKPGSIGIPLPGTEVKIVDDDGEELPpGE-VGELWV 326
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 527 KKswPGAFRTLYGDHERYETTyFKpfAGYYFTGDGCSRDKDGYHWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAA 606
Cdd:cd05936 327 RG--PQVMKGYWNRPEETAEA-FV--DGWLRTGDIGYMDEDGYFFIVDRKKDMIIVGGFNVYPREVEEVLYEHPAVAEAA 401
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 607 VVGVEHEVKGQGIYAFVTLVDGVPYSEElrkSLIMTVRNQIGAFAAPDKIHWAPGLPKTRSGKIMRRILR 676
Cdd:cd05936 402 VVGVPDPYSGEAVKAFVVLKEGASLTEE---EIIAFCREQLAGYKVPRQVEFRDELPKSAVGKILRRELR 468
|
|
| FACL_FadD13-like |
cd17631 |
fatty acyl-CoA synthetase, including FadD13; This family contains fatty acyl-CoA synthetases, ... |
143-672 |
1.41e-58 |
|
fatty acyl-CoA synthetase, including FadD13; This family contains fatty acyl-CoA synthetases, including Mycobacterium tuberculosis acid-induced operon MymA encoding the fatty acyl-CoA synthetase FadD13 which is essential for virulence and intracellular growth of the pathogen. The fatty acyl-CoA synthetase activates lipids before entering into the metabolic pathways and is also involved in transmembrane lipid transport. However, unlike soluble fatty acyl-CoA synthetases, but like the mammalian integral-membrane very-long-chain acyl-CoA synthetases, FadD13 accepts lipid substrates up to the maximum length of C26, and this is facilitated by an extensive hydrophobic tunnel from the active site to a positively charged patch. Also included is feruloyl-CoA synthetase (Fcs) in Rhodococcus strains where it is involved in biotechnological vanillin production from eugenol and ferulic acid via a non-beta-oxidative pathway.
Pssm-ID: 341286 [Multi-domain] Cd Length: 435 Bit Score: 204.38 E-value: 1.41e-58
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 143 NGDKIAMYWEGNEpsqdgkLTYSELLEKVCQLANYLKSLGVGKGDAVIIYLPMLLELPIAMLACARIGAVHSVVFAGFSA 222
Cdd:cd17631 8 HPDRTALVFGGRS------LTYAELDERVNRLAHALRALGVAKGDRVAVLSKNSPEFLELLFAAARLGAVFVPLNFRLTP 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 223 DSLAQRIVDCKPKLVItcnavkrgvkpiflkdivdvalvesekngvsvglcltyenqsamkredtkwqperdvwwqdvvt 302
Cdd:cd17631 82 PEVAYILADSGAKVLF---------------------------------------------------------------- 97
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 303 kfptkcdvewvdaEDPLFLLYTSGSTGKPKGVLHTSGGYMVYTATTFkYAFDYKPTDIYWCTADCGWITGHSYVTYGPLL 382
Cdd:cd17631 98 -------------DDLALLMYTSGTTGRPKGAMLTHRNLLWNAVNAL-AALDLGPDDVLLVVAPLFHIGGLGVFTLPTLL 163
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 383 NGATVLVFEGtpnyPDSGRCWDIVDKYNVTIFYTAPTLVRSLMRDGTEYVTRYSrkSLRVLGSVGEPINPS---AWRWFY 459
Cdd:cd17631 164 RGGTVVILRK----FDPETVLDLIERHRVTSFFLVPTMIQALLQHPRFATTDLS--SLRAVIYGGAPMPERllrALQARG 237
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 460 nivgdsrCPISDTWWQTETGGfMITPLPGAWPQ-KPGSATFPFFGVQPVIVDEKGQEIEGECSGYLCIKKswPGAFRTLY 538
Cdd:cd17631 238 -------VKFVQGYGMTETSP-GVTFLSPEDHRrKLGSAGRPVFFVEVRIVDPDGREVPPGEVGEIVVRG--PHVMAGYW 307
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 539 GDHERYETTYFKpfaGYYFTGDGCSRDKDGYHWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAAVVGVEHEVKGQG 618
Cdd:cd17631 308 NRPEATAAAFRD---GWFHTGDLGRLDEDGYLYIVDRKKDMIISGGENVYPAEVEDVLYEHPAVAEVAVIGVPDEKWGEA 384
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....
gi 242061796 619 IYAFVTLVDGVPYSEElrkSLIMTVRNQIGAFAAPDKIHWAPGLPKTRSGKIMR 672
Cdd:cd17631 385 VVAVVVPRPGAELDED---ELIAHCRERLARYKIPKSVEFVDALPRNATGKILK 435
|
|
| FACL_fum10p_like |
cd05926 |
Subfamily of fatty acid CoA ligase (FACL) similar to Fum10p of Gibberella moniliformis; FACL ... |
156-677 |
2.30e-54 |
|
Subfamily of fatty acid CoA ligase (FACL) similar to Fum10p of Gibberella moniliformis; FACL catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, followed by the formation of a fatty acyl-CoA. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions. Fum10p is a fatty acid CoA ligase involved in the synthesis of fumonisin, a polyketide mycotoxin, in Gibberella moniliformis.
Pssm-ID: 341249 [Multi-domain] Cd Length: 493 Bit Score: 194.45 E-value: 2.30e-54
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 156 PSQDGKLTYSELLEKVCQLANYLKSLGVGKGDAVIIYLPMLLELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPK 235
Cdd:cd05926 9 PGSTPALTYADLAELVDDLARQLAALGIKKGDRVAIALPNGLEFVVAFLAAARAGAVVAPLNPAYKKAEFEFYLADLGSK 88
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 236 LVITCN-----AVKRGVKP-IFLKDI-VDVALVESEKNGVSVGLCLtyenqsamkrEDTKWQPERDVwwqdvvtkfptkc 308
Cdd:cd05926 89 LVLTPKgelgpASRAASKLgLAILELaLDVGVLIRAPSAESLSNLL----------ADKKNAKSEGV------------- 145
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 309 dvewVDAEDPLFLLYTSGSTGKPKGVLHTSGGyMVYTATTFKYAFDYKPTDIYWCTADCGWITGHSYVTYGPLLNGATVL 388
Cdd:cd05926 146 ----PLPDDLALILHTSGTTGRPKGVPLTHRN-LAASATNITNTYKLTPDDRTLVVMPLFHVHGLVASLLSTLAAGGSVV 220
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 389 VfegtPNYPDSGRCWDIVDKYNVTiFYTA-PTLVRS-LMRDGTEYVTRYSrkSLRVLGSVGEPINPSAWRWFYNIVGdsr 466
Cdd:cd05926 221 L----PPRFSASTFWPDVRDYNAT-WYTAvPTIHQIlLNRPEPNPESPPP--KLRFIRSCSASLPPAVLEALEATFG--- 290
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 467 CPISDTWWQTETGGFMIT-PLPGAwPQKPGSATFPFfGVQPVIVDEKGQEIEGECSGYLCIKKswPGAFRTLYGDHERYE 545
Cdd:cd05926 291 APVLEAYGMTEAAHQMTSnPLPPG-PRKPGSVGKPV-GVEVRILDEDGEILPPGVVGEICLRG--PNVTRGYLNNPEANA 366
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 546 TTYFKpfAGYYFTGDGCSRDKDGYHWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAAVVGVEHEVKGQGIYAFVTL 625
Cdd:cd05926 367 EAAFK--DGWFRTGDLGYLDADGYLFLTGRIKELINRGGEKISPLEVDGVLLSHPAVLEAVAFGVPDEKYGEEVAAAVVL 444
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|..
gi 242061796 626 VDGVPYSEElrkSLIMTVRNQIGAFAAPDKIHWAPGLPKTRSGKIMRRILRK 677
Cdd:cd05926 445 REGASVTEE---ELRAFCRKHLAAFKVPKKVYFVDELPKTATGKIQRRKVAE 493
|
|
| PRK08316 |
PRK08316 |
acyl-CoA synthetase; Validated |
145-681 |
1.39e-53 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 181381 [Multi-domain] Cd Length: 523 Bit Score: 192.84 E-value: 1.39e-53
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 145 DKIAMYWEgnepsqDGKLTYSELLEKVCQLANYLKSLGVGKGDAVIIYLPMLLELPIAMLACARIGAVHSVVFAGFSADS 224
Cdd:PRK08316 26 DKTALVFG------DRSWTYAELDAAVNRVAAALLDLGLKKGDRVAALGHNSDAYALLWLACARAGAVHVPVNFMLTGEE 99
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 225 LAQRIVDCKPKLVItcnavkrgvkpiflkdiVDVALVESEKNGVSvglcltyenqsAMKREDTKWQP-----ERDVWWQD 299
Cdd:PRK08316 100 LAYILDHSGARAFL-----------------VDPALAPTAEAALA-----------LLPVDTLILSLvlggrEAPGGWLD 151
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 300 V----VTKFPTKCDVEwVDAEDPLFLLYTSGSTGKPKGVLHTSGGYMV-YTATTFkyAFDYKPTDI-------YWCTAdc 367
Cdd:PRK08316 152 FadwaEAGSVAEPDVE-LADDDLAQILYTSGTESLPKGAMLTHRALIAeYVSCIV--AGDMSADDIplhalplYHCAQ-- 226
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 368 gwitghSYVTYGP-LLNGATVLVFEGtpnyPDSGRCWDIVDKYNVTIFYTAPTLVRSLMR----DgteyvtRYSRKSLR- 441
Cdd:PRK08316 227 ------LDVFLGPyLYVGATNVILDA----PDPELILRTIEAERITSFFAPPTVWISLLRhpdfD------TRDLSSLRk 290
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 442 -----------VLGSVGEPInPSAWrwFYNIVGdsrcpisdtwwQTEtggfmITPL-----PGAWPQKPGSATFPFFGVQ 505
Cdd:PRK08316 291 gyygasimpveVLKELRERL-PGLR--FYNCYG-----------QTE-----IAPLatvlgPEEHLRRPGSAGRPVLNVE 351
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 506 PVIVDEKGQEI-EGE-----------CSGYlcikkswpgafrtlYGDHERYETTyfkpFAGYYF-TGDGCSRDKDGYHWL 572
Cdd:PRK08316 352 TRVVDDDGNDVaPGEvgeivhrspqlMLGY--------------WDDPEKTAEA----FRGGWFhSGDLGVMDEEGYITV 413
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 573 TGRVDDVINVSGHRIGTAEVESALVSHPQCAEAAVVGVEHEVKGQGIYAFVTLVDGVPYSEElrkSLIMTVRNQIGAFAA 652
Cdd:PRK08316 414 VDRKKDMIKTGGENVASREVEEALYTHPAVAEVAVIGLPDPKWIEAVTAVVVPKAGATVTED---ELIAHCRARLAGFKV 490
|
570 580
....*....|....*....|....*....
gi 242061796 653 PDKIHWAPGLPKTRSGKIMRRILRKIAAR 681
Cdd:PRK08316 491 PKRVIFVDELPRNPSGKILKRELRERYAG 519
|
|
| Firefly_Luc_like |
cd05911 |
Firefly luciferase of light emitting insects and 4-Coumarate-CoA Ligase (4CL); This family ... |
161-671 |
2.83e-53 |
|
Firefly luciferase of light emitting insects and 4-Coumarate-CoA Ligase (4CL); This family contains insect firefly luciferases that share significant sequence similarity to plant 4-coumarate:coenzyme A ligases, despite their functional diversity. Luciferase catalyzes the production of light in the presence of MgATP, molecular oxygen, and luciferin. In the first step, luciferin is activated by acylation of its carboxylate group with ATP, resulting in an enzyme-bound luciferyl adenylate. In the second step, luciferyl adenylate reacts with molecular oxygen, producing an enzyme-bound excited state product (Luc=O*) and releasing AMP. This excited-state product then decays to the ground state (Luc=O), emitting a quantum of visible light.
Pssm-ID: 341237 [Multi-domain] Cd Length: 486 Bit Score: 191.27 E-value: 2.83e-53
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 161 KLTYSELLEKVCQLANYLKSLGVGKGDAVIIYLPMLLELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPKLVITC 240
Cdd:cd05911 10 ELTYAQLRTLSRRLAAGLRKLGLKKGDVVGIISPNSTYYPPVFLGCLFAGGIFSAANPIYTADELAHQLKISKPKVIFTD 89
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 241 navkrgvkpiflKDIVDVALvESEKNGVSVG--LCLTYENQSAMKRED-TKWQPERDVWWQDVVTKfptkcdvewVDAED 317
Cdd:cd05911 90 ------------PDGLEKVK-EAAKELGPKDkiIVLDDKPDGVLSIEDlLSPTLGEEDEDLPPPLK---------DGKDD 147
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 318 PLFLLYTSGSTGKPKGVL--H---TSGGYMVYTAttFKYAFDYKPTDIYWCTADcgWITG-HSYVTYgpLLNGATVLVFe 391
Cdd:cd05911 148 TAAILYSSGTTGLPKGVClsHrnlIANLSQVQTF--LYGNDGSNDVILGFLPLY--HIYGlFTTLAS--LLNGATVIIM- 220
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 392 gtpNYPDSGRCWDIVDKYNVTIFYTAPTLVRSLMRDGTeyVTRYSRKSLRVLGSVGEPInpsaWRWFYNIVG--DSRCPI 469
Cdd:cd05911 221 ---PKFDSELFLDLIEKYKITFLYLVPPIAAALAKSPL--LDKYDLSSLRVILSGGAPL----SKELQELLAkrFPNATI 291
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 470 SDTWWQTETGGfMITPLPGaWPQKPGSATFPFFGVQPVIVDEKGQEIEGECS-GYLCIKKswPGAFRTLYGDHERYETTY 548
Cdd:cd05911 292 KQGYGMTETGG-ILTVNPD-GDDKPGSVGRLLPNVEAKIVDDDGKDSLGPNEpGEICVRG--PQVMKGYYNNPEATKETF 367
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 549 FKpfAGYYFTGDGCSRDKDGYHWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAAVVGVEHEVKGQGIYAFVTLVDG 628
Cdd:cd05911 368 DE--DGWLHTGDIGYFDEDGYLYIVDRKKELIKYKGFQVAPAELEAVLLEHPGVADAAVIGIPDEVSGELPRAYVVRKPG 445
|
490 500 510 520
....*....|....*....|....*....|....*....|....*...
gi 242061796 629 vpySEELRKSLIMTVRNQIGAFaapdK-----IHWAPGLPKTRSGKIM 671
Cdd:cd05911 446 ---EKLTEKEVKDYVAKKVASY----KqlrggVVFVDEIPKSASGKIL 486
|
|
| MACS_like_1 |
cd05974 |
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the ... |
162-679 |
2.42e-52 |
|
Uncharacterized subfamily of medium-chain acyl-CoA synthetase (MACS); MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. MACS enzymes are localized to mitochondria.
Pssm-ID: 341278 [Multi-domain] Cd Length: 432 Bit Score: 187.39 E-value: 2.42e-52
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 162 LTYSELLEKVCQLANYLKSLGVGKGDAVIIYLPMLLELPIAMLACARIGAVHSVVFAGFSADSLAQRivdckpklvitcn 241
Cdd:cd05974 1 VSFAEMSARSSRVANFLRSIGVGRGDRILLMLGNVVELWEAMLAAMKLGAVVIPATTLLTPDDLRDR------------- 67
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 242 aVKRGVKPIflkdivdvalvesekngvsvglcltyenqsAMKREDTKwqperdvwwqdvvtkfptkcdvewvdAEDPLFL 321
Cdd:cd05974 68 -VDRGGAVY------------------------------AAVDENTH--------------------------ADDPMLL 90
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 322 LYTSGSTGKPKGVLHTSGGYMVYTATTFkYAFDYKPTDIYWCTADCGWiTGHSYVT-YGPLLNGATVLVFegtpNYP--D 398
Cdd:cd05974 91 YFTSGTTSKPKLVEHTHRSYPVGHLSTM-YWIGLKPGDVHWNISSPGW-AKHAWSCfFAPWNAGATVFLF----NYArfD 164
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 399 SGRCWDIVDKYNVTIFYTAPTLVRSLMRdgtEYVTRYSRKSLRVLGSvGEPINPSAWRWFYNIVGDSrcpISDTWWQTET 478
Cdd:cd05974 165 AKRVLAALVRYGVTTLCAPPTVWRMLIQ---QDLASFDVKLREVVGA-GEPLNPEVIEQVRRAWGLT---IRDGYGQTET 237
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 479 GGfMITPLPGAwPQKPGSATFPFFGVQPVIVDEKGQEI-EGECSgyLCIKKSWPGAFRTLY-GDHERyetTYFKPFAGYY 556
Cdd:cd05974 238 TA-LVGNSPGQ-PVKAGSMGRPLPGYRVALLDPDGAPAtEGEVA--LDLGDTRPVGLMKGYaGDPDK---TAHAMRGGYY 310
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 557 FTGDGCSRDKDGYHWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAAVVGVEHEVKGQGIYAFVTLVDGVPYSEELR 636
Cdd:cd05974 311 RTGDIAMRDEDGYLTYVGRADDVFKSSDYRISPFELESVLIEHPAVAEAAVVPSPDPVRLSVPKAFIVLRAGYEPSPETA 390
|
490 500 510 520
....*....|....*....|....*....|....*....|...
gi 242061796 637 KSLIMTVRNQIGAFAAPDKIHWAPgLPKTRSGKIMRRILRKIA 679
Cdd:cd05974 391 LEIFRFSRERLAPYKRIRRLEFAE-LPKTISGKIRRVELRRRE 432
|
|
| benz_CoA_lig |
TIGR02262 |
benzoate-CoA ligase family; Characterized members of this protein family include benzoate-CoA ... |
132-677 |
1.03e-51 |
|
benzoate-CoA ligase family; Characterized members of this protein family include benzoate-CoA ligase, 4-hydroxybenzoate-CoA ligase, 2-aminobenzoate-CoA ligase, etc. Members are related to fatty acid and acetate CoA ligases.
Pssm-ID: 274059 [Multi-domain] Cd Length: 505 Bit Score: 187.35 E-value: 1.03e-51
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 132 YNA----VDRNIESGNGDKIAMYwegnepSQDGKLTYSELLEKVCQLANYLKSLGVGKGDAVIIYLPMLLELPIAMLACA 207
Cdd:TIGR02262 3 YNAaedlLDRNVVEGRGGKTAFI------DDISSLSYGELEAQVRRLAAALRRLGVKREERVLLLMLDGVDFPIAFLGAI 76
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 208 RIGAVHSVVFAGFSADSLAQRIVDCKPKLVITCNAVKRGVKPIflkdivdVALVESEKNGVSVGLCLTYENQSAMKREDT 287
Cdd:TIGR02262 77 RAGIVPVALNTLLTADDYAYMLEDSRARVVFVSGALLPVIKAA-------LGKSPHLEHRVVVGRPEAGEVQLAELLATE 149
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 288 kwqperdvwwQDVVTKFPTKCDvewvdaeDPLFLLYTSGSTGKPKGVLHTSGGYMVYTATTFKYAFDYKPTDIYWCTADC 367
Cdd:TIGR02262 150 ----------SEQFKPAATQAD-------DPAFWLYSSGSTGMPKGVVHTHSNPYWTAELYARNTLGIREDDVCFSAAKL 212
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 368 GWITGHSYVTYGPLLNGATVLVFegtPNYPDSGRCWDIVDKYNVTIFYTAPTLVRSLMRDgtEYVTRYSRKSLRVLGSVG 447
Cdd:TIGR02262 213 FFAYGLGNALTFPMSVGATTVLM---GERPTPDAVFDRLRRHQPTIFYGVPTLYAAMLAD--PNLPSEDQVRLRLCTSAG 287
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 448 EPINPSAWRWFYNIVGDSrcpISDTWWQTETGGFMITPLPGAwpQKPGSATFPFFGVQPVIVDEKGQEIEGECSGYLCIk 527
Cdd:TIGR02262 288 EALPAEVGQRWQARFGVD---IVDGIGSTEMLHIFLSNLPGD--VRYGTSGKPVPGYRLRLVGDGGQDVADGEPGELLI- 361
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 528 kSWPGAFRTLYGDHERYETTYFKPFAGyyfTGDGCSRDKDGYHWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAAV 607
Cdd:TIGR02262 362 -SGPSSATMYWNNRAKSRDTFQGEWTR---SGDKYVRNDDGSYTYAGRTDDMLKVSGIYVSPFEIESALIQHPAVLEAAV 437
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 608 VGVEHEVKGQGIYAFVTLVDGvpySEELRKSLIMTVRNQIGAFAAPDKIHWAPGLPKTRSGKIMRRILRK 677
Cdd:TIGR02262 438 VGVADEDGLIKPKAFVVLRPG---QTALETELKEHVKDRLAPYKYPRWIVFVDDLPKTATGKIQRFKLRE 504
|
|
| A_NRPS |
cd05930 |
The adenylation domain of nonribosomal peptide synthetases (NRPS); The adenylation (A) domain ... |
159-673 |
1.51e-51 |
|
The adenylation domain of nonribosomal peptide synthetases (NRPS); The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341253 [Multi-domain] Cd Length: 444 Bit Score: 185.42 E-value: 1.51e-51
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 159 DGKLTYSELLEKVCQLANYLKSLGVGKGDAVIIYLPMLLELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPKLVI 238
Cdd:cd05930 10 DQSLTYAELDARANRLARYLRERGVGPGDLVAVLLERSLEMVVAILAVLKAGAAYVPLDPSYPAERLAYILEDSGAKLVL 89
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 239 TcnavkrgvkpiflkdivdvalvesekngvsvglcltyenqsamkredtkwqperdvwwqdvvtkfptkcdvewvDAEDP 318
Cdd:cd05930 90 T--------------------------------------------------------------------------DPDDL 95
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 319 LFLLYTSGSTGKPKGVLHTSGGYmVYTATTFKYAFDYKPTDIYWCTADCGWItGHSYVTYGPLLNGATVLVFEGTPNYpD 398
Cdd:cd05930 96 AYVIYTSGSTGKPKGVMVEHRGL-VNLLLWMQEAYPLTPGDRVLQFTSFSFD-VSVWEIFGALLAGATLVVLPEEVRK-D 172
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 399 SGRCWDIVDKYNVTIFYTAPTLVRSLMrdgtEYVTRYSRKSLRVLGSVGEPINPSAWR-WF--------YNIVGDSRCPI 469
Cdd:cd05930 173 PEALADLLAEEGITVLHLTPSLLRLLL----QELELAALPSLRLVLVGGEALPPDLVRrWRellpgarlVNLYGPTEATV 248
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 470 SDTWWQTETGGFMITPLP-GAwpqkpgsatfPFFGVQPVIVDEKGQE----IEGEcsgyLCIkkSWPGAFRtlyGDHERY 544
Cdd:cd05930 249 DATYYRVPPDDEEDGRVPiGR----------PIPNTRVYVLDENLRPvppgVPGE----LYI--GGAGLAR---GYLNRP 309
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 545 ETTYFK----PFAG---YYFTGDGCSRDKDG---YHwltGRVDDVINVSGHRIGTAEVESALVSHPQCAEAAVVGVEHEV 614
Cdd:cd05930 310 ELTAERfvpnPFGPgerMYRTGDLVRWLPDGnleFL---GRIDDQVKIRGYRIELGEIEAALLAHPGVREAAVVAREDGD 386
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 615 KGQGIYAFVTLVDGVPYS-EELRKSLimtvRNQIGAFAAPDKIHWAPGLPKTRSGKIMRR 673
Cdd:cd05930 387 GEKRLVAYVVPDEGGELDeEELRAHL----AERLPDYMVPSAFVVLDALPLTPNGKVDRK 442
|
|
| FACL_DitJ_like |
cd05934 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ... |
159-676 |
8.00e-51 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions. Members of this family include DitJ from Pseudomonas and similar proteins.
Pssm-ID: 341257 [Multi-domain] Cd Length: 422 Bit Score: 182.88 E-value: 8.00e-51
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 159 DGKLTYSELLEKVCQLANYLKSLGVGKGDAVIIYLPMLLELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPKLVI 238
Cdd:cd05934 1 GRRWTYAELLRESARIAAALAALGIRPGDRVALMLDNCPEFLFAWFALAKLGAVLVPINTALRGDELAYIIDHSGAQLVV 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 239 TcnavkrgvkpiflkdivdvalvesekngvsvglcltyenqsamkredtkwqperdvwwqdvvtkfptkcdvewvdaeDP 318
Cdd:cd05934 81 V-----------------------------------------------------------------------------DP 83
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 319 LFLLYTSGSTGKPKGVLHTSGgYMVYTATTFKYAFDYKPTDIYWCTADCGWITGHSYVTYGPLLNGATVLVfegTPNYPD 398
Cdd:cd05934 84 ASILYTSGTTGPPKGVVITHA-NLTFAGYYSARRFGLGEDDVYLTVLPLFHINAQAVSVLAALSVGATLVL---LPRFSA 159
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 399 SgRCWDIVDKYNVTIFYTAPTLVRSLMRdgTEYVTRYSRKSLRVLGsvGEPINPSAWRWFYNIVGdsrCPISDTWWQTET 478
Cdd:cd05934 160 S-RFWSDVRRYGATVTNYLGAMLSYLLA--QPPSPDDRAHRLRAAY--GAPNPPELHEEFEERFG---VRLLEGYGMTET 231
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 479 GGFMITPLPGawPQKPGSATFPFFGVQPVIVDEKGQEIEGECSGYLCIKKSWP-GAFRTLYGDHERYEttyfKPFA-GYY 556
Cdd:cd05934 232 IVGVIGPRDE--PRRPGSIGRPAPGYEVRIVDDDGQELPAGEPGELVIRGLRGwGFFKGYYNMPEATA----EAMRnGWF 305
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 557 FTGDGCSRDKDGYHWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAAVVGVEHEVKGQGIYAFVTLVDGVPYSEElr 636
Cdd:cd05934 306 HTGDLGYRDADGFFYFVDRKKDMIRRRGENISSAEVERAILRHPAVREAAVVAVPDEVGEDEVKAVVVLRPGETLDPE-- 383
|
490 500 510 520
....*....|....*....|....*....|....*....|
gi 242061796 637 kSLIMTVRNQIGAFAAPDKIHWAPGLPKTRSGKIMRRILR 676
Cdd:cd05934 384 -ELFAFCEGQLAYFKVPRYIRFVDDLPKTPTEKVAKAQLR 422
|
|
| CHC_CoA_lg |
cd05903 |
Cyclohexanecarboxylate-CoA ligase (also called cyclohex-1-ene-1-carboxylate:CoA ligase); ... |
162-677 |
6.11e-46 |
|
Cyclohexanecarboxylate-CoA ligase (also called cyclohex-1-ene-1-carboxylate:CoA ligase); Cyclohexanecarboxylate-CoA ligase activates the aliphatic ring compound, cyclohexanecarboxylate, for degradation. It catalyzes the synthesis of cyclohexanecarboxylate-CoA thioesters in a two-step reaction involving the formation of cyclohexanecarboxylate-AMP anhydride, followed by the nucleophilic substitution of AMP by CoA.
Pssm-ID: 341229 [Multi-domain] Cd Length: 437 Bit Score: 169.48 E-value: 6.11e-46
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 162 LTYSELLEKVCQLANYLKSLGVGKGDAVIIYLPMLLELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPKLVITCN 241
Cdd:cd05903 2 LTYSELDTRADRLAAGLAALGVGPGDVVAFQLPNWWEFAVLYLACLRIGAVTNPILPFFREHELAFILRRAKAKVFVVPE 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 242 AVKRgvkpiflkdivdvalvesekngvsvglcltyenqsamkredTKWQPERDvwwqdvvtkfptkcdvewvdaeDPLFL 321
Cdd:cd05903 82 RFRQ-----------------------------------------FDPAAMPD----------------------AVALL 98
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 322 LYTSGSTGKPKGVLHTSGGYMvytATTFKYAFDY--KPTDIYWCTADCGWITGHSYVTYGPLLNGATVLVFEGTpnypDS 399
Cdd:cd05903 99 LFTSGTTGEPKGVMHSHNTLS---ASIRQYAERLglGPGDVFLVASPMAHQTGFVYGFTLPLLLGAPVVLQDIW----DP 171
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 400 GRCWDIVDKYNVTIFYTAPTLVRSLMRDGTEYVTRYSRksLRVLGSVGEPINPSAWRWFYNIVGDSRCPIsdtWWQTETG 479
Cdd:cd05903 172 DKALALMREHGVTFMMGATPFLTDLLNAVEEAGEPLSR--LRTFVCGGATVPRSLARRAAELLGAKVCSA---YGSTECP 246
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 480 GFMITPLPGAWPQKPGSATFPFFGVQPVIVDEKGQEIEGECSGYLCIKKswPGAFrtlYGDHERYETTYFKPFAGYYFTG 559
Cdd:cd05903 247 GAVTSITPAPEDRRLYTDGRPLPGVEIKVVDDTGATLAPGVEGELLSRG--PSVF---LGYLDRPDLTADAAPEGWFRTG 321
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 560 DGCSRDKDGYHWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAAVVGVEHEVKGQGIYAFVTLVDG-VPYSEELRKS 638
Cdd:cd05903 322 DLARLDEDGYLRITGRSKDIIIRGGENIPVLEVEDLLLGHPGVIEAAVVALPDERLGERACAVVVTKSGaLLTFDELVAY 401
|
490 500 510
....*....|....*....|....*....|....*....
gi 242061796 639 LimtVRNQIGAFAAPDKIHWAPGLPKTRSGKIMRRILRK 677
Cdd:cd05903 402 L---DRQGVAKQYWPERLVHVDDLPRTPSGKVQKFRLRE 437
|
|
| PRK07529 |
PRK07529 |
AMP-binding domain protein; Validated |
162-699 |
1.05e-44 |
|
AMP-binding domain protein; Validated
Pssm-ID: 236043 [Multi-domain] Cd Length: 632 Bit Score: 169.75 E-value: 1.05e-44
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 162 LTYSELLEKVCQLANYLKSLGVGKGDAVIIYLPMLLELPIAMLAcariGAVHSVVFA---GFSADSLAQRIVDCKPKLVI 238
Cdd:PRK07529 59 WTYAELLADVTRTANLLHSLGVGPGDVVAFLLPNLPETHFALWG----GEAAGIANPinpLLEPEQIAELLRAAGAKVLV 134
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 239 TcnavkrgVKPIFLKDIVD-----VALVESEKNGVSVGL--CLTYENQSAMKREDTKwQPERDVWWQDVVTKFPTKC--D 309
Cdd:PRK07529 135 T-------LGPFPGTDIWQkvaevLAALPELRTVVEVDLarYLPGPKRLAVPLIRRK-AHARILDFDAELARQPGDRlfS 206
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 310 VEWVDAEDPLFLLYTSGSTGKPKGVLHTSGGyMVYTATTFKYAFDYKPTDiywcTADCGWITGH---SYVT-YGPLLNGA 385
Cdd:PRK07529 207 GRPIGPDDVAAYFHTGGTTGMPKLAQHTHGN-EVANAWLGALLLGLGPGD----TVFCGLPLFHvnaLLVTgLAPLARGA 281
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 386 TVLVfeGTP-NYPDSG---RCWDIVDKYNVTIFYTAPTLVRSLMRdgtEYVTRYSRKSLRVLGSVGEPINPSAWRWFYNI 461
Cdd:PRK07529 282 HVVL--ATPqGYRGPGviaNFWKIVERYRINFLSGVPTVYAALLQ---VPVDGHDISSLRYALCGAAPLPVEVFRRFEAA 356
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 462 VGdsrCPISDTWWQTE-TGGFMITPLPGawPQKPGSA--TFPFFGVQPVIVDEKGQEIEgECS----GYLCIkkSWPGAF 534
Cdd:PRK07529 357 TG---VRIVEGYGLTEaTCVSSVNPPDG--ERRIGSVglRLPYQRVRVVILDDAGRYLR-DCAvdevGVLCI--AGPNVF 428
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 535 RT-LYGDHER---YETTYFKpfagyyfTGDGCSRDKDGYHWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAAVVGV 610
Cdd:PRK07529 429 SGyLEAAHNKglwLEDGWLN-------TGDLGRIDADGYFWLTGRAKDLIIRGGHNIDPAAIEEALLRHPAVALAAAVGR 501
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 611 EHEVKGQGIYAFVTLVDGVPYSEElrkSLIMTVRNQIGAFAA-PDKIHWAPGLPKTRSGKIMRRILRKIAARQ-----LD 684
Cdd:PRK07529 502 PDAHAGELPVAYVQLKPGASATEA---ELLAFARDHIAERAAvPKHVRILDALPKTAVGKIFKPALRRDAIRRvlraaLR 578
|
570
....*....|....*
gi 242061796 685 ELGdistlAEPAVVD 699
Cdd:PRK07529 579 DAG-----VEAEVVD 588
|
|
| ABCL |
cd05958 |
2-aminobenzoate-CoA ligase (ABCL); ABCL catalyzes the initial step in the 2-aminobenzoate ... |
308-676 |
4.89e-44 |
|
2-aminobenzoate-CoA ligase (ABCL); ABCL catalyzes the initial step in the 2-aminobenzoate aerobic degradation pathway by activating 2-aminobenzoate to 2-aminobenzoyl-CoA. The reaction is carried out via a two-step process; the first step is ATP-dependent and forms a 2-aminobenzoyl-AMP intermediate, and the second step forms the 2-aminobenzoyl-CoA ester and releases the AMP. 2-Aminobenzoyl-CoA is further converted to 2-amino-5-oxo-cyclohex-1-ene-1-carbonyl-CoA catalyzed by 2-aminobenzoyl-CoA monooxygenase/reductase. ABCL has been purified from cells aerobically grown with 2-aminobenzoate as sole carbon, energy, and nitrogen source, and has been characterized as a monomer.
Pssm-ID: 341268 [Multi-domain] Cd Length: 439 Bit Score: 164.19 E-value: 4.89e-44
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 308 CDVEWVDAEDPLFLLYTSGSTGKPKGVLHTSGGYMVYTATTFKYAFDYKPTDIYWCTADCGWITGHSYVTYGPLLNGATV 387
Cdd:cd05958 89 CAHALTASDDICILAFTSGTTGAPKATMHFHRDPLASADRYAVNVLRLREDDRFVGSPPLAFTFGLGGVLLFPFGVGASG 168
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 388 LVFEGTPnyPDsgRCWDIVDKYNVTIFYTAPTLVRSLMRDGTEYVTRYSrkSLRVLGSVGEPINPSAWRWFYNIVGdsrC 467
Cdd:cd05958 169 VLLEEAT--PD--LLLSAIARYKPTVLFTAPTAYRAMLAHPDAAGPDLS--SLRKCVSAGEALPAALHRAWKEATG---I 239
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 468 PISDTWWQTETGGFMITPLPGAwpQKPGSATFPFFGVQPVIVDEKGQEIEGECSGYLCIKKswPGAFRtlyGDHERYETT 547
Cdd:cd05958 240 PIIDGIGSTEMFHIFISARPGD--ARPGATGKPVPGYEAKVVDDEGNPVPDGTIGRLAVRG--PTGCR---YLADKRQRT 312
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 548 YFKpfAGYYFTGDGCSRDKDGYHWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAAVVGVEHEVKGQGIYAFVTLVD 627
Cdd:cd05958 313 YVQ--GGWNITGDTYSRDPDGYFRHQGRSDDMIVSGGYNIAPPEVEDVLLQHPAVAECAVVGHPDESRGVVVKAFVVLRP 390
|
330 340 350 360
....*....|....*....|....*....|....*....|....*....
gi 242061796 628 GVPYSEELRKSLIMTVRNQIGAFAAPDKIHWAPGLPKTRSGKIMRRILR 676
Cdd:cd05958 391 GVIPGPVLARELQDHAKAHIAPYKYPRAIEFVTELPRTATGKLQRFALR 439
|
|
| 4CL |
cd05904 |
4-Coumarate-CoA Ligase (4CL); 4-Coumarate:coenzyme A ligase is a key enzyme in the ... |
162-675 |
1.96e-43 |
|
4-Coumarate-CoA Ligase (4CL); 4-Coumarate:coenzyme A ligase is a key enzyme in the phenylpropanoid metabolic pathway for monolignol and flavonoid biosynthesis. It catalyzes the synthesis of hydroxycinnamate-CoA thioesters in a two-step reaction, involving the formation of hydroxycinnamate-AMP anhydride and the nucleophilic substitution of AMP by CoA. The phenylpropanoid pathway is one of the most important secondary metabolism pathways in plants and hydroxycinnamate-CoA thioesters are the precursors of lignin and other important phenylpropanoids.
Pssm-ID: 341230 [Multi-domain] Cd Length: 505 Bit Score: 163.94 E-value: 1.96e-43
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 162 LTYSELLEKVCQLANYLKSLGVGKGDAVIIYLPMLLELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPKLVITCN 241
Cdd:cd05904 33 LTYAELERRVRRLAAGLAKRGGRKGDVVLLLSPNSIEFPVAFLAVLSLGAVVTTANPLSTPAEIAKQVKDSGAKLAFTTA 112
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 242 AVKRGVKPIflkdivdvalveseknGVSVGLCLTYENQSAMKREDTKWQPERDvwwqdvvtkfptkCDVEWVDAEDPLFL 321
Cdd:cd05904 113 ELAEKLASL----------------ALPVVLLDSAEFDSLSFSDLLFEADEAE-------------PPVVVIKQDDVAAL 163
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 322 LYTSGSTGKPKGVLHTSGGY--MVyTATTFKYAFDYKPTDIYWCTADCGWITGHSYVTYGPLLNGATVLVFegtPNYpDS 399
Cdd:cd05904 164 LYSSGTTGRSKGVMLTHRNLiaMV-AQFVAGEGSNSDSEDVFLCVLPMFHIYGLSSFALGLLRLGATVVVM---PRF-DL 238
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 400 GRCWDIVDKYNVTIFYTAPTLVRSLMRDGTeyVTRYSRKSLRVLGSVGEPINPSAWRWFYNIVGDsrCPISDTWWQTE-T 478
Cdd:cd05904 239 EELLAAIERYKVTHLPVVPPIVLALVKSPI--VDKYDLSSLRQIMSGAAPLGKELIEAFRAKFPN--VDLGQGYGMTEsT 314
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 479 GGFMITPLPGAWPQKPGSATFPFFGVQPVIVDEKGQEIEGEC-SGYLCIKKswPGAFRTLYGDHERYETTYFKpfAGYYF 557
Cdd:cd05904 315 GVVAMCFAPEKDRAKYGSVGRLVPNVEAKIVDPETGESLPPNqTGELWIRG--PSIMKGYLNNPEATAATIDK--EGWLH 390
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 558 TGDGCSRDKDGYHWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAAVVGVEHEVKGQGIYAFVTLVDGVPYSEelrK 637
Cdd:cd05904 391 TGDLCYIDEDGYLFIVDRLKELIKYKGFQVAPAELEALLLSHPEILDAAVIPYPDEEAGEVPMAFVVRKPGSSLTE---D 467
|
490 500 510 520
....*....|....*....|....*....|....*....|..
gi 242061796 638 SLIMTVRNQIgafaAPDK----IHWAPGLPKTRSGKIMRRIL 675
Cdd:cd05904 468 EIMDFVAKQV----APYKkvrkVAFVDAIPKSPSGKILRKEL 505
|
|
| PRK07656 |
PRK07656 |
long-chain-fatty-acid--CoA ligase; Validated |
144-677 |
2.10e-42 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 236072 [Multi-domain] Cd Length: 513 Bit Score: 161.23 E-value: 2.10e-42
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 144 GDKIAmYWEGnepsqDGKLTYSELLEKVCQLANYLKSLGVGKGDAVIIYLPMLLELPIAMLACARIGAVHSVVFAGFSAD 223
Cdd:PRK07656 19 GDKEA-YVFG-----DQRLTYAELNARVRRAAAALAALGIGKGDRVAIWAPNSPHWVIAALGALKAGAVVVPLNTRYTAD 92
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 224 SLAQRIVDCKPKLVITCNAvkrgvkpiFLKdiVDVALVESEKNGVSVGLCLTYENQSAMKREDTkwqperdvwWQDVVTK 303
Cdd:PRK07656 93 EAAYILARGDAKALFVLGL--------FLG--VDYSATTRLPALEHVVICETEEDDPHTEKMKT---------FTDFLAA 153
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 304 FPTKCDVEWVDAEDPLFLLYTSGSTGKPKGVLHTSGG-YMVYTATTfKYAfDYKPTDIYWC----------TAdcGWITg 372
Cdd:PRK07656 154 GDPAERAPEVDPDDVADILFTSGTTGRPKGAMLTHRQlLSNAADWA-EYL-GLTEGDRYLAanpffhvfgyKA--GVNA- 228
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 373 hsyvtygPLLNGATVL---VFegtpnypDSGRCWDIVDKYNVTIFYTAPTLVRSLMR--DGTEYvtrySRKSLRVLGSVG 447
Cdd:PRK07656 229 -------PLMRGATILplpVF-------DPDEVFRLIETERITVLPGPPTMYNSLLQhpDRSAE----DLSSLRLAVTGA 290
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 448 EPINPSAWRWFYNIVGDSRcpISDTWWQTETGGFM-ITPLPGAWPQKPGSATFPFFGVQPVIVDEKGQEIEGECSGYLCI 526
Cdd:PRK07656 291 ASMPVALLERFESELGVDI--VLTGYGLSEASGVTtFNRLDDDRKTVAGTIGTAIAGVENKIVNELGEEVPVGEVGELLV 368
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 527 KKswPGAFRTLYGDHEryETTYFKPFAGYYFTGDGCSRDKDGYHWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAA 606
Cdd:PRK07656 369 RG--PNVMKGYYDDPE--ATAAAIDADGWLHTGDLGRLDEEGYLYIVDRKKDMFIVGGFNVYPAEVEEVLYEHPAVAEAA 444
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 242061796 607 VVGVEHEVKGQGIYAFVTLVDGVPYSEElrkSLIMTVRNQIGAFAAPDKIHWAPGLPKTRSGKIMRRILRK 677
Cdd:PRK07656 445 VIGVPDERLGEVGKAYVVLKPGAELTEE---ELIAYCREHLAKYKVPRSIEFLDELPKNATGKVLKRALRE 512
|
|
| A_NRPS_VisG_like |
cd17651 |
similar to adenylation domain of virginiamycin S synthetase; This family of the adenylation (A) ... |
145-676 |
2.21e-42 |
|
similar to adenylation domain of virginiamycin S synthetase; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes virginiamycin S synthetase (VisG) in Streptomyces virginiae; VisG is involved in virginiamycin S (VS) biosynthesis as the provider of an L-pheGly molecule, a highly specific substrate for the last condensation step by VisF. This family also includes linear gramicidin synthetase B (LgrB) in Brevibacillus brevis. Substrate specificity analysis using residues of the substrate-binding pockets of all 16 adenylation domains has shown good agreement of the substrate amino acids predicted with the sequence of linear gramicidin. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341306 [Multi-domain] Cd Length: 491 Bit Score: 160.59 E-value: 2.21e-42
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 145 DKIAMYWEGnepsqdGKLTYSELLEKVCQLANYLKSLGVGKGDAVIIYLPMLLELPIAMLACARIGAVHSVVFAGFSADS 224
Cdd:cd17651 10 DAPALVAEG------RRLTYAELDRRANRLAHRLRARGVGPGDLVALCARRSAELVVALLAILKAGAAYVPLDPAYPAER 83
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 225 LAQRIVDCKPKLVITCNAVKrgvkpiflkdivDVALVEsekngvsvglcltyenqsamkredtkwqpERDVWWQDV---V 301
Cdd:cd17651 84 LAFMLADAGPVLVLTHPALA------------GELAVE-----------------------------LVAVTLLDQpgaA 122
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 302 TKFPTKCDVEwVDAEDPLFLLYTSGSTGKPKGVL------------HTSGGYMVYTATTFKYA---FDYKPTDIywctad 366
Cdd:cd17651 123 AGADAEPDPA-LDADDLAYVIYTSGSTGRPKGVVmphrslanlvawQARASSLGPGARTLQFAglgFDVSVQEI------ 195
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 367 cgwitghsyvtYGPLLNGATvLVFEGTPNYPDSGRCWDIVDKYNVTIFYTAPTLVRSLMRDGTEYVTRYSrkSLRVLGSV 446
Cdd:cd17651 196 -----------FSTLCAGAT-LVLPPEEVRTDPPALAAWLDEQRISRVFLPTVALRALAEHGRPLGVRLA--ALRYLLTG 261
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 447 GEP-INPSAWRWFYNIVGDSRcpISDTWWQTET---GGFMITPLPGAWPQKPGSATfPFFGVQPVIVDEKGQEIEGECSG 522
Cdd:cd17651 262 GEQlVLTEDLREFCAGLPGLR--LHNHYGPTEThvvTALSLPGDPAAWPAPPPIGR-PIDNTRVYVLDAALRPVPPGVPG 338
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 523 YLCIkkSWPGAFRTLYGD----HERYETTYFKPFAGYYFTGDGCSRDKDG-YHWLtGRVDDVINVSGHRIGTAEVESALV 597
Cdd:cd17651 339 ELYI--GGAGLARGYLNRpeltAERFVPDPFVPGARMYRTGDLARWLPDGeLEFL-GRADDQVKIRGFRIELGEIEAALA 415
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 598 SHPQCAEAAVVGVEHEVKGQGIYAFVTLVDGVPYS-EELRKSLimtvRNQIGAFAAPDKIHWAPGLPKTRSGKIMRRILR 676
Cdd:cd17651 416 RHPGVREAVVLAREDRPGEKRLVAYVVGDPEAPVDaAELRAAL----ATHLPEYMVPSAFVLLDALPLTPNGKLDRRALP 491
|
|
| A_NRPS_Ta1_like |
cd12116 |
The adenylation domain of nonribosomal peptide synthetases (NRPS), including salinosporamide A ... |
159-675 |
9.38e-42 |
|
The adenylation domain of nonribosomal peptide synthetases (NRPS), including salinosporamide A polyketide synthase; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions. This family includes the myxovirescin (TA) antibiotic biosynthetic gene in Myxococcus xanthus; TA production plays a role in predation. It also includes the salinosporamide A polyketide synthase which is involved in the biosynthesis of salinosporamide A, a marine microbial metabolite whose chlorine atom is crucial for potent proteasome inhibition and anticancer activity.
Pssm-ID: 341281 [Multi-domain] Cd Length: 470 Bit Score: 158.61 E-value: 9.38e-42
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 159 DGKLTYSELLEKVCQLANYLKSLGVGKGDAVIIYLPMLLELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPKLVI 238
Cdd:cd12116 10 DRSLSYAELDERANRLAARLRARGVGPGDRVAVYLPRSARLVAAMLAVLKAGAAYVPLDPDYPADRLRYILEDAEPALVL 89
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 239 TcnavkrgvkpiflkdivDVALVEseknGVSVGLCLTYENQSAMKREDTKWQPErdvwwqdvvtkfptkcdvewVDAEDP 318
Cdd:cd12116 90 T-----------------DDALPD----RLPAGLPVLLLALAAAAAAPAAPRTP--------------------VSPDDL 128
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 319 LFLLYTSGSTGKPKGV-----------------LHTSGGYMVYTATTfkYAFDYKPTDIYWctadcgwitghsyvtygPL 381
Cdd:cd12116 129 AYVIYTSGSTGRPKGVvvshrnlvnflhsmrerLGLGPGDRLLAVTT--YAFDISLLELLL-----------------PL 189
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 382 LNGATVLVFEGTPNYpDSGRCWDIVDKYNVTIFYTAPTLVRSLMRDGTEyvtrySRKSLRVL-GsvGEPINP-------S 453
Cdd:cd12116 190 LAGARVVIAPRETQR-DPEALARLIEAHSITVMQATPATWRMLLDAGWQ-----GRAGLTALcG--GEALPPdlaarllS 261
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 454 AWRWFYNIVGdsrcPISDTWWQTetggfmITPLPGAWPQKPgsATFPFFGVQPVIVDEKGQEI-EGECsGYLCIkkSWPG 532
Cdd:cd12116 262 RVGSLWNLYG----PTETTIWST------AARVTAAAGPIP--IGRPLANTQVYVLDAALRPVpPGVP-GELYI--GGDG 326
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 533 AFRtlyGDHERYETTYFK----PFAG----YYFTGDGCSRDKDG---YHwltGRVDDVINVSGHRIGTAEVESALVSHPQ 601
Cdd:cd12116 327 VAQ---GYLGRPALTAERfvpdPFAGpgsrLYRTGDLVRRRADGrleYL---GRADGQVKIRGHRIELGEIEAALAAHPG 400
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 242061796 602 CAEAAVVgVEHEVKGQGIYAFVTLVDGVPYS-EELRKSLimtvRNQIGAFAAPDKIHWAPGLPKTRSGKIMRRIL 675
Cdd:cd12116 401 VAQAAVV-VREDGGDRRLVAYVVLKAGAAPDaAALRAHL----RATLPAYMVPSAFVRLDALPLTANGKLDRKAL 470
|
|
| AA-adenyl-dom |
TIGR01733 |
amino acid adenylation domain; This model represents a domain responsible for the specific ... |
163-607 |
1.19e-40 |
|
amino acid adenylation domain; This model represents a domain responsible for the specific recognition of amino acids and activation as adenylyl amino acids. The reaction catalyzed is aa + ATP -> aa-AMP + PPi. These domains are usually found as components of multi-domain non-ribosomal peptide synthetases and are usually called "A-domains" in that context. A-domains are almost invariably followed by "T-domains" (thiolation domains, pfam00550) to which the amino acid adenylate is transferred as a thiol-ester to a bound pantetheine cofactor with the release of AMP (these are also called peptide carrier proteins, or PCPs. When the A-domain does not represent the first module (corresponding to the first amino acid in the product molecule) it is usually preceded by a "C-domain" (condensation domain, pfam00668) which catalyzes the ligation of two amino acid thiol-esters from neighboring modules. This domain is a subset of the AMP-binding domain found in Pfam (pfam00501) which also hits substrate--CoA ligases and luciferases. Sequences scoring in between trusted and noise for this model may be ambiguous as to whether they activate amino acids or other molecules lacking an alpha amino group.
Pssm-ID: 273779 [Multi-domain] Cd Length: 409 Bit Score: 153.96 E-value: 1.19e-40
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 163 TYSELLEKVCQLANYLKSL-GVGKGDAVIIYLPMLLELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPKLVITCn 241
Cdd:TIGR01733 1 TYRELDERANRLARHLRAAgGVGPGDRVAVLLERSAELVVAILAVLKAGAAYVPLDPAYPAERLAFILEDAGARLLLTD- 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 242 avkrgvkPIFLKDIVDVALVesekngvsvGLCLTyenqsamkredtkwQPERDVWWQDVVTKFPTkcdvEWVDAEDPLFL 321
Cdd:TIGR01733 80 -------SALASRLAGLVLP---------VILLD--------------PLELAALDDAPAPPPPD----APSGPDDLAYV 125
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 322 LYTSGSTGKPKGVLHTSGGyMVYTATTFKYAFDYKPTDIywctadcgWITGHSYV-------TYGPLLNGATVLVFEGTP 394
Cdd:TIGR01733 126 IYTSGSTGRPKGVVVTHRS-LVNLLAWLARRYGLDPDDR--------VLQFASLSfdasveeIFGALLAGATLVVPPEDE 196
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 395 NYPDSGRCWDIVDKYNVTIFYTAPTLVRSLMRDGteyvtRYSRKSLRVLGSVGEPINPSA---WRW------FYNIVGds 465
Cdd:TIGR01733 197 ERDDAALLAALIAEHPVTVLNLTPSLLALLAAAL-----PPALASLRLVILGGEALTPALvdrWRArgpgarLINLYG-- 269
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 466 rcPisdtwwqTE-TGGFMITPLPGAWPQKPGSATF--PFFGVQPVIVDEKGQEIEGECSGYLCIkkSWPGAFRTLYGDHE 542
Cdd:TIGR01733 270 --P-------TEtTVWSTATLVDPDDAPRESPVPIgrPLANTRLYVLDDDLRPVPVGVVGELYI--GGPGVARGYLNRPE 338
|
410 420 430 440 450 460 470
....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 242061796 543 RYETTYFK-PFAG-----YYFTGDGCSRDKDGYHWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAAV 607
Cdd:TIGR01733 339 LTAERFVPdPFAGgdgarLYRTGDLVRYLPDGNLEFLGRIDDQVKIRGYRIELGEIEAALLRHPGVREAVV 409
|
|
| A_NRPS_Srf_like |
cd12117 |
The adenylation domain of nonribosomal peptide synthetases (NRPS), including Bacillus subtilis ... |
159-675 |
1.03e-39 |
|
The adenylation domain of nonribosomal peptide synthetases (NRPS), including Bacillus subtilis termination module Surfactin (SrfA-C); The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and, in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions. This family includes the adenylation domain of the Bacillus subtilis termination module (Surfactin domain, SrfA-C) which recognizes a specific amino acid building block, which is then activated and transferred to the terminal thiol of the 4'-phosphopantetheine (Ppan) arm of the downstream peptidyl carrier protein (PCP) domain.
Pssm-ID: 341282 [Multi-domain] Cd Length: 483 Bit Score: 152.74 E-value: 1.03e-39
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 159 DGKLTYSELLEKVCQLANYLKSLGVGKGDAVIIYLPMLLELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPKLVI 238
Cdd:cd12117 20 DRSLTYAELNERANRLARRLRAAGVGPGDVVGVLAERSPELVVALLAVLKAGAAYVPLDPELPAERLAFMLADAGAKVLL 99
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 239 TCNAVKRGVKPIFLKDIVDVALVESEKNGVSVGlcltyenqsamkredtkwqperdvwwqdvvtkfptkcdvewVDAEDP 318
Cdd:cd12117 100 TDRSLAGRAGGLEVAVVIDEALDAGPAGNPAVP-----------------------------------------VSPDDL 138
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 319 LFLLYTSGSTGKPKGV---------LHTSGGYMVYTA-TTF----KYAFDYKPTDIYwctadcgwitghsyvtyGPLLNG 384
Cdd:cd12117 139 AYVMYTSGSTGRPKGVavthrgvvrLVKNTNYVTLGPdDRVlqtsPLAFDASTFEIW-----------------GALLNG 201
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 385 AT-VLVFEGTPNYPDSGRcwDIVDKYNVT-IFYTAPtLVRSLMRDGTEYVTrysrkSLRVLGSVGEPINPSAWRWFYNIV 462
Cdd:cd12117 202 ARlVLAPKGTLLDPDALG--ALIAEEGVTvLWLTAA-LFNQLADEDPECFA-----GLRELLTGGEVVSPPHVRRVLAAC 273
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 463 GDSRcpISDTWWQTETGGF----MITPLPGAWPQKP-GSatfPFFGVQPVIVDEKGQEIEGECSGYLCIkkSWPGAFRTL 537
Cdd:cd12117 274 PGLR--LVNGYGPTENTTFttshVVTELDEVAGSIPiGR---PIANTRVYVLDEDGRPVPPGVPGELYV--GGDGLALGY 346
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 538 YGD----HERYETTYFKPFAGYYFTGDGCSRDKDGYHWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAAVVGVEHE 613
Cdd:cd12117 347 LNRpaltAERFVADPFGPGERLYRTGDLARWLPDGRLEFLGRIDDQVKIRGFRIELGEIEAALRAHPGVREAVVVVREDA 426
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|..
gi 242061796 614 VKGQGIYAFVTLVDGVPySEELRKSLimtvRNQIGAFAAPDKIHWAPGLPKTRSGKIMRRIL 675
Cdd:cd12117 427 GGDKRLVAYVVAEGALD-AAELRAFL----RERLPAYMVPAAFVVLDELPLTANGKVDRRAL 483
|
|
| PLN03052 |
PLN03052 |
acetate--CoA ligase; Provisional |
73-683 |
5.72e-39 |
|
acetate--CoA ligase; Provisional
Pssm-ID: 215553 [Multi-domain] Cd Length: 728 Bit Score: 154.08 E-value: 5.72e-39
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 73 YREMYERSIKNPAGFWSEIAETFYWKEKWNPAEVCSENLDVTKGPVqinWFKGGKTNICYNAVDRNIESgNGDKIAMYW- 151
Cdd:PLN03052 122 FSEFQRFSVENPEVYWSIVLDELSLVFSVPPRCILDTSDESNPGGQ---WLPGAVLNVAECCLTPKPSK-TDDSIAIIWr 197
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 152 -EGNEPSQDGKLTYSELLEKVCQLANYLKSLGVGKGDAVIIYLPMLLELPIAMLACARIGAVHSVVFAGFSADSLAQRIV 230
Cdd:PLN03052 198 dEGSDDLPVNRMTLSELRSQVSRVANALDALGFEKGDAIAIDMPMNVHAVIIYLAIILAGCVVVSIADSFAPSEIATRLK 277
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 231 DCKPKLVITCNAVKRGVKPIFL-KDIVDVA-----LVESEKNGVSVGLcltyenqsamkREDtkwqperDVWWQDVVTKF 304
Cdd:PLN03052 278 ISKAKAIFTQDVIVRGGKSIPLySRVVEAKapkaiVLPADGKSVRVKL-----------REG-------DMSWDDFLARA 339
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 305 -PTKCDVEWVDAEDPL----FLLYTSGSTGKPKGVLHTSggymvytATTFKYA------FDYKPTDIY-WCTaDCGWITG 372
Cdd:PLN03052 340 nGLRRPDEYKAVEQPVeaftNILFSSGTTGEPKAIPWTQ-------LTPLRAAadawahLDIRKGDIVcWPT-NLGWMMG 411
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 373 HsYVTYGPLLNGATVLVFEGTPNYPDSGRcwdIVDKYNVTIFYTAPTLVRSLMRdgTEYVTRYSRKSLRVLGSVGEPINP 452
Cdd:PLN03052 412 P-WLVYASLLNGATLALYNGSPLGRGFAK---FVQDAKVTMLGTVPSIVKTWKN--TNCMAGLDWSSIRCFGSTGEASSV 485
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 453 SAWRWFYnivgdSRC---PISDTWWQTETGGFMITplpGAWPQKPGSATF--PFFGVQPVIVDEKGQEI--EGECSGYLC 525
Cdd:PLN03052 486 DDYLWLM-----SRAgykPIIEYCGGTELGGGFVT---GSLLQPQAFAAFstPAMGCKLFILDDSGNPYpdDAPCTGELA 557
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 526 IKKSWPGAFRTLY-GDHERyetTYFKPFAGYYFT-----GDGCSRDKDGYHWLTGRVDDVINVSGHRIGTAEVESAL-VS 598
Cdd:PLN03052 558 LFPLMFGASSTLLnADHYK---VYFKGMPVFNGKilrrhGDIFERTSGGYYRAHGRADDTMNLGGIKVSSVEIERVCnAA 634
|
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 599 HPQCAEAAVVGVEHevKGQGIYAFVTLV------DGVPYSEELRKSLIMTVRNQIGAFAAPDKIHWAPGLPKTRSGKIMR 672
Cdd:PLN03052 635 DESVLETAAIGVPP--PGGGPEQLVIAAvlkdppGSNPDLNELKKIFNSAIQKKLNPLFKVSAVVIVPSFPRTASNKVMR 712
|
650
....*....|.
gi 242061796 673 RILRKIAARQL 683
Cdd:PLN03052 713 RVLRQQLAQEL 723
|
|
| EntE |
COG1021 |
EntE, 2,3-dihydroxybenzoate-AMP synthase component of non-ribosomal peptide synthetase ... |
159-681 |
2.16e-38 |
|
EntE, 2,3-dihydroxybenzoate-AMP synthase component of non-ribosomal peptide synthetase [Secondary metabolites biosynthesis, transport and catabolism];
Pssm-ID: 440644 [Multi-domain] Cd Length: 533 Bit Score: 149.91 E-value: 2.16e-38
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 159 DGKLTYSELLEKVCQLANYLKSLGVGKGDAVIIYLPMLLELPIAMLACARIGAVhsVVFAGFS-----ADSLAQRIvdcK 233
Cdd:COG1021 48 ERRLSYAELDRRADRLAAGLLALGLRPGDRVVVQLPNVAEFVIVFFALFRAGAI--PVFALPAhrraeISHFAEQS---E 122
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 234 PKLVITCnAVKRGvkpiF-LKDIVD--VALVESEKNGVSVGlcltyenqsamkredtkwQPERDVWWQDVVTKfPTKCDV 310
Cdd:COG1021 123 AVAYIIP-DRHRG----FdYRALARelQAEVPSLRHVLVVG------------------DAGEFTSLDALLAA-PADLSE 178
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 311 EWVDAEDPLFLLYTSGSTGKPKGVLHTSGGYmVYTATTFKYAFDYKPTDIYWCTADcgwiTGHSY-----VTYGPLLNGA 385
Cdd:COG1021 179 PRPDPDDVAFFQLSGGTTGLPKLIPRTHDDY-LYSVRASAEICGLDADTVYLAALP----AAHNFplsspGVLGVLYAGG 253
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 386 TVlVFEGTPnYPDsgRCWDIVDKYNVTIfyTA--PTLVRSLMRDGTEYvtRYSRKSLRVLGSVGEPINPSAWR------- 456
Cdd:COG1021 254 TV-VLAPDP-SPD--TAFPLIERERVTV--TAlvPPLALLWLDAAERS--RYDLSSLRVLQVGGAKLSPELARrvrpalg 325
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 457 -----WF--------YNIVGDSR--------CPIS--DTWWqtetggfmitplpgawpqkpgsatfpffgvqpvIVDEKG 513
Cdd:COG1021 326 ctlqqVFgmaeglvnYTRLDDPEevilttqgRPISpdDEVR---------------------------------IVDEDG 372
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 514 QEI-EGECsGYLCIKKswPGAFRTLYGDHErYETTYFKPfAGYYFTGDGCSRDKDGYHWLTGRVDDVINVSGHRIGTAEV 592
Cdd:COG1021 373 NPVpPGEV-GELLTRG--PYTIRGYYRAPE-HNARAFTP-DGFYRTGDLVRRTPDGYLVVEGRAKDQINRGGEKIAAEEV 447
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 593 ESALVSHPQCAEAAVVGVEHEVKGQGIYAFVTLVDGVPYSEELRKSLimtvRNQ-IGAFAAPDKIHWAPGLPKTRSGKIM 671
Cdd:COG1021 448 ENLLLAHPAVHDAAVVAMPDEYLGERSCAFVVPRGEPLTLAELRRFL----RERgLAAFKLPDRLEFVDALPLTAVGKID 523
|
570
....*....|
gi 242061796 672 RRILRKIAAR 681
Cdd:COG1021 524 KKALRAALAA 533
|
|
| MCS |
cd05941 |
Malonyl-CoA synthetase (MCS); MCS catalyzes the formation of malonyl-CoA in a two-step ... |
317-677 |
8.62e-38 |
|
Malonyl-CoA synthetase (MCS); MCS catalyzes the formation of malonyl-CoA in a two-step reaction consisting of the adenylation of malonate with ATP, followed by malonyl transfer from malonyl-AMP to CoA. Malonic acid and its derivatives are the building blocks of polyketides and malonyl-CoA serves as the substrate of polyketide synthases. Malonyl-CoA synthetase has broad substrate tolerance and can activate a variety of malonyl acid derivatives. MCS may play an important role in biosynthesis of polyketides, the important secondary metabolites with therapeutic and agrochemical utility.
Pssm-ID: 341264 [Multi-domain] Cd Length: 442 Bit Score: 146.28 E-value: 8.62e-38
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 317 DPLFLLYTSGSTGKPKGVLHTSGGyMVYTATTFKYAFDYKPTD-IYWCTAdcgWITGHSYVT--YGPLLNGATVlvfEGT 393
Cdd:cd05941 90 DPALILYTSGTTGRPKGVVLTHAN-LAANVRALVDAWRWTEDDvLLHVLP---LHHVHGLVNalLCPLFAGASV---EFL 162
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 394 PNYpDSGRCWDIVDKYNVTIFYTAPTLVRSLMRD------GTEYVTRYSRKSLRVLGSVGEPINPSAWRWFYNIVGDsrc 467
Cdd:cd05941 163 PKF-DPKEVAISRLMPSITVFMGVPTIYTRLLQYyeahftDPQFARAAAAERLRLMVSGSAALPVPTLEEWEAITGH--- 238
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 468 PISDTWWQTETGGFMITPLPGawPQKPGSATFPFFGVQPVIVD-EKGQEIEGECSGYLCIKKswPGAFRTLYGDHERYEt 546
Cdd:cd05941 239 TLLERYGMTEIGMALSNPLDG--ERRPGTVGMPLPGVQARIVDeETGEPLPRGEVGEIQVRG--PSVFKEYWNKPEATK- 313
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 547 tyfKPFA--GYYFTGDGCSRDKDGYHWLTGRV-DDVINVSGHRIGTAEVESALVSHPQCAEAAVVGVEHEVKGQGIYAFV 623
Cdd:cd05941 314 ---EEFTddGWFKTGDLGVVDEDGYYWILGRSsVDIIKSGGYKVSALEIERVLLAHPGVSECAVIGVPDPDWGERVVAVV 390
|
330 340 350 360 370
....*....|....*....|....*....|....*....|....*....|....*.
gi 242061796 624 TLVDGVP--YSEELRKSLimtvRNQIGAFAAPDKIHWAPGLPKTRSGKIMRRILRK 677
Cdd:cd05941 391 VLRAGAAalSLEELKEWA----KQRLAPYKRPRRLILVDELPRNAMGKVNKKELRK 442
|
|
| 23DHB-AMP_lg |
cd05920 |
2,3-dihydroxybenzoate-AMP ligase; 2,3-dihydroxybenzoate-AMP ligase activates 2, ... |
159-675 |
1.79e-37 |
|
2,3-dihydroxybenzoate-AMP ligase; 2,3-dihydroxybenzoate-AMP ligase activates 2,3-dihydroxybenzoate (DHB) by ligation of AMP from ATP with the release of pyrophosphate. However, it can also catalyze the ATP-PPi exchange for 2,3-DHB analogs, such as salicyclic acid (o-hydrobenzoate), as well as 2,4-DHB and 2,5-DHB, but with less efficiency. Proteins in this family are the stand-alone adenylation components of non-ribosomal peptide synthases (NRPSs) involved in the biosynthesis of siderophores, which are low molecular weight iron-chelating compounds synthesized by many bacteria to aid in the acquisition of this vital trace elements. In Escherichia coli, the 2,3-dihydroxybenzoate-AMP ligase is called EntE, the adenylation component of the enterobactin NRPS system.
Pssm-ID: 341244 [Multi-domain] Cd Length: 482 Bit Score: 146.32 E-value: 1.79e-37
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 159 DGKLTYSELLEKVCQLANYLKSLGVGKGDAVIIYLPMLLELPIAMLACARIGAVhsVVFAGFSadslaQRIVDCkpklvi 238
Cdd:cd05920 38 DRRLTYRELDRRADRLAAGLRGLGIRPGDRVVVQLPNVAEFVVLFFALLRLGAV--PVLALPS-----HRRSEL------ 104
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 239 tcNAVKRGVKPiflkdivdVALVESEKNGVSVGLcltyenqsAMKREDTKWQPerdvwwqdvvtkfptkcdvewvdaeDP 318
Cdd:cd05920 105 --SAFCAHAEA--------VAYIVPDRHAGFDHR--------ALARELAESIP-------------------------EV 141
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 319 LFLLYTSGSTGKPKGVLHTSGGYmVYTATTFKYAFDYKPTDIYWCTADcgwiTGHSYVTYGP-----LLNGATVLVfegT 393
Cdd:cd05920 142 ALFLLSGGTTGTPKLIPRTHNDY-AYNVRASAEVCGLDQDTVYLAVLP----AAHNFPLACPgvlgtLLAGGRVVL---A 213
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 394 PNyPDSGRCWDIVDKYNVTifytAPTLVRSLMRDGTEYVTRYSR--KSLRVLGSVGEPINPSAWR------------WF- 458
Cdd:cd05920 214 PD-PSPDAAFPLIEREGVT----VTALVPALVSLWLDAAASRRAdlSSLRLLQVGGARLSPALARrvppvlgctlqqVFg 288
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 459 -------YNIVGDSRCPIsdtwwqTETGGFMITPLPGAWpqkpgsatfpffgvqpvIVDEKGQEI-EGEcSGYLCIKKsw 530
Cdd:cd05920 289 maegllnYTRLDDPDEVI------IHTQGRPMSPDDEIR-----------------VVDEEGNPVpPGE-EGELLTRG-- 342
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 531 PGAFRTLYgDHERYETTYFKPfAGYYFTGDGCSRDKDGYHWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAAVVGV 610
Cdd:cd05920 343 PYTIRGYY-RAPEHNARAFTP-DGFYRTGDLVRRTPDGYLVVEGRIKDQINRGGEKIAAEEVENLLLRHPAVHDAAVVAM 420
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 242061796 611 EHEVKGQGIYAFVTLVDGVPYSEELRKSLimtVRNQIGAFAAPDKIHWAPGLPKTRSGKIMRRIL 675
Cdd:cd05920 421 PDELLGERSCAFVVLRDPPPSAAQLRRFL---RERGLAAYKLPDRIEFVDSLPLTAVGKIDKKAL 482
|
|
| PRK13391 |
PRK13391 |
acyl-CoA synthetase; Provisional |
156-676 |
2.80e-37 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 184022 [Multi-domain] Cd Length: 511 Bit Score: 146.37 E-value: 2.80e-37
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 156 PSQDGKLTYSELLEKVCQLANYLKSLGVGKGDAVIIYLPMLLELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPK 235
Cdd:PRK13391 19 ASTGEVVTYRELDERSNRLAHLFRSLGLKRGDHVAIFMENNLRYLEVCWAAERSGLYYTCVNSHLTPAEAAYIVDDSGAR 98
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 236 LVITCNAvKRGVKPIFLKDIVDVALvesekngvsvglCLTYENQSAMKRedtkWQPerdvwWQDVVTKFP-TKCDVEWVD 314
Cdd:PRK13391 99 ALITSAA-KLDVARALLKQCPGVRH------------RLVLDGDGELEG----FVG-----YAEAVAGLPaTPIADESLG 156
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 315 AEdplfLLYTSGSTGKPKGVLHTSGGYMVYTATT----FKYAFDYKPTDIYWCTADCGWITGHSYVTYGPLLnGATVLVF 390
Cdd:PRK13391 157 TD----MLYSSGTTGRPKGIKRPLPEQPPDTPLPltafLQRLWGFRSDMVYLSPAPLYHSAPQRAVMLVIRL-GGTVIVM 231
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 391 EgtpnYPDSGRCWDIVDKYNVTIFYTAPTLVRSLMRDGTEYVTRYSRKSLRVLGSVGEPINPS------AWrWfynivgd 464
Cdd:PRK13391 232 E----HFDAEQYLALIEEYGVTHTQLVPTMFSRMLKLPEEVRDKYDLSSLEVAIHAAAPCPPQvkeqmiDW-W------- 299
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 465 srCPISDTWW-QTETGGFMITPlPGAWPQKPGSATFPFFGVqPVIVDEKGQEIEGECSGYLCIKKSWPgaFRTLYGDHER 543
Cdd:PRK13391 300 --GPIIHEYYaATEGLGFTACD-SEEWLAHPGTVGRAMFGD-LHILDDDGAELPPGEPGTIWFEGGRP--FEYLNDPAKT 373
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 544 YETTYfkPFAGYYFTGDGCSRDKDGYHWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAAVVGVEHEVKGQGIYAFV 623
Cdd:PRK13391 374 AEARH--PDGTWSTVGDIGYVDEDGYLYLTDRAAFMIISGGVNIYPQEAENLLITHPKVADAAVFGVPNEDLGEEVKAVV 451
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|...
gi 242061796 624 TLVDGVPYSEELRKSLIMTVRNQIGAFAAPDKIHWAPGLPKTRSGKIMRRILR 676
Cdd:PRK13391 452 QPVDGVDPGPALAAELIAFCRQRLSRQKCPRSIDFEDELPRLPTGKLYKRLLR 504
|
|
| PRK08314 |
PRK08314 |
long-chain-fatty-acid--CoA ligase; Validated |
145-675 |
3.09e-37 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 236235 [Multi-domain] Cd Length: 546 Bit Score: 146.64 E-value: 3.09e-37
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 145 DKIAMYWEGNEpsqdgkLTYSELLEKVCQLANYLKS-LGVGKGDAVIIYLPMLLELPIAMLACARIGAVHSVVFAGFSAD 223
Cdd:PRK08314 25 DKTAIVFYGRA------ISYRELLEEAERLAGYLQQeCGVRKGDRVLLYMQNSPQFVIAYYAILRANAVVVPVNPMNREE 98
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 224 SLAQRIVDCKPKLVITCNAVKRGVKPIFLKDIVDVALVESEKNGVSVGLCLTYENQSAMKREDTKWQPERDVWWQDVVTK 303
Cdd:PRK08314 99 ELAHYVTDSGARVAIVGSELAPKVAPAVGNLRLRHVIVAQYSDYLPAEPEIAVPAWLRAEPPLQALAPGGVVAWKEALAA 178
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 304 FPTKCDVEwVDAEDPLFLLYTSGSTGKPKGVLHTSGGYMVyTATTFKYAFDYKPTDIYWCTADCGWITGHSYVTYGPLLN 383
Cdd:PRK08314 179 GLAPPPHT-AGPDDLAVLPYTSGTTGVPKGCMHTHRTVMA-NAVGSVLWSNSTPESVVLAVLPLFHVTGMVHSMNAPIYA 256
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 384 GATVLVFEgtpnypdsgRcWD------IVDKYNVTIFYTAPTLVRSLMrdGTEYVTRYSRKSLRVLGSVGEPINPSAWRW 457
Cdd:PRK08314 257 GATVVLMP---------R-WDreaaarLIERYRVTHWTNIPTMVVDFL--ASPGLAERDLSSLRYIGGGGAAMPEAVAER 324
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 458 FYNIVGdsrCPISDTWWQTETGGFMITPLPGAwpQKPGSATFPFFGVQPVIVD-EKGQEI----EGEcsgylcIKKSWPG 532
Cdd:PRK08314 325 LKELTG---LDYVEGYGLTETMAQTHSNPPDR--PKLQCLGIPTFGVDARVIDpETLEELppgeVGE------IVVHGPQ 393
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 533 AFRTLYGDHERYETTyFKPFAGYYF--TGDGCSRDKDGYHWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAAVVGV 610
Cdd:PRK08314 394 VFKGYWNRPEATAEA-FIEIDGKRFfrTGDLGRMDEEGYFFITDRLKRMINASGFKVWPAEVENLLYKHPAIQEACVIAT 472
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 242061796 611 EHEVKGQGIYAFVTLVD---GVPYSEElrksLIMTVRNQIGAFAAPDKIHWAPGLPKTRSGKIMRRIL 675
Cdd:PRK08314 473 PDPRRGETVKAVVVLRPearGKTTEEE----IIAWAREHMAAYKYPRIVEFVDSLPKSGSGKILWRQL 536
|
|
| A_NRPS_Sfm_like |
cd12115 |
The adenylation domain of nonribosomal peptide synthetases (NRPS), including Saframycin A gene ... |
145-675 |
2.24e-36 |
|
The adenylation domain of nonribosomal peptide synthetases (NRPS), including Saframycin A gene cluster from Streptomyces lavendulae; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions. This family includes the saframycin A gene cluster from Streptomyces lavendulae which implicates the NRPS system for assembling the unusual tetrapeptidyl skeleton in an iterative manner. It also includes saframycin Mx1 produced by Myxococcus xanthus NRPS.
Pssm-ID: 341280 [Multi-domain] Cd Length: 447 Bit Score: 142.46 E-value: 2.24e-36
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 145 DKIAMYwegnepSQDGKLTYSELLEKVCQLANYLKSLGVGKGDAVIIYLPMLLELPIAMLACARIGAVHSVVFAGFSADS 224
Cdd:cd12115 14 DAIALV------CGDESLTYAELNRRANRLAARLRAAGVGPESRVGVCLERTPDLVVALLAVLKAGAAYVPLDPAYPPER 87
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 225 LAQRIVDCKPKLVITcnavkrgvkpiflkdivdvalvesekngvsvglcltyenqsamkredtkwqperdvwwqdvvtkf 304
Cdd:cd12115 88 LRFILEDAQARLVLT----------------------------------------------------------------- 102
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 305 ptkcdvewvDAEDPLFLLYTSGSTGKPKGVLHTSGGymvytATTFkyafdykptdIYWCTADCG--WITGHSYVT----- 377
Cdd:cd12115 103 ---------DPDDLAYVIYTSGSTGRPKGVAIEHRN-----AAAF----------LQWAAAAFSaeELAGVLASTsicfd 158
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 378 ------YGPLLNGATVLVFEGTPNYPDSGRCWDivdkynVTIFYTAPTLVRSLMR-DGTEyvtrysrKSLRVLGSVGEPI 450
Cdd:cd12115 159 lsvfelFGPLATGGKVVLADNVLALPDLPAAAE------VTLINTVPSAAAELLRhDALP-------ASVRVVNLAGEPL 225
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 451 NPSAWRWFY---------NIVGdsrcPISDTWWQTetgGFMITPLPGAWPqkpgSATFPFFGVQPVIVDEKGQEIEGECS 521
Cdd:cd12115 226 PRDLVQRLYarlqvervvNLYG----PSEDTTYST---VAPVPPGASGEV----SIGRPLANTQAYVLDRALQPVPLGVP 294
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 522 GYLCIkkSWPGAFRTLYGD----HERYETTYFKPFAGYYFTGDGCSRDKDGYHWLTGRVDDVINVSGHRIGTAEVESALV 597
Cdd:cd12115 295 GELYI--GGAGVARGYLGRpgltAERFLPDPFGPGARLYRTGDLVRWRPDGLLEFLGRADNQVKVRGFRIELGEIEAALR 372
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 242061796 598 SHPQCAEAAVVGVEHEVKGQGIYAFVTLVDGV-PYSEELRKSLimtvRNQIGAFAAPDKIHWAPGLPKTRSGKIMRRIL 675
Cdd:cd12115 373 SIPGVREAVVVAIGDAAGERRLVAYIVAEPGAaGLVEDLRRHL----GTRLPAYMVPSRFVRLDALPLTPNGKIDRSAL 447
|
|
| PLN03051 |
PLN03051 |
acyl-activating enzyme; Provisional |
187-676 |
3.56e-36 |
|
acyl-activating enzyme; Provisional
Pssm-ID: 215552 [Multi-domain] Cd Length: 499 Bit Score: 143.03 E-value: 3.56e-36
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 187 DAVIIYLPMLLElpiamlacariGAVHSVVFAGFSADSLAQRIVDCKPKLVITCNAVKRGVKPIFLKDIVdvalVESEKN 266
Cdd:PLN03051 6 DAVIIYLAIVLA-----------GCVVVSVADSFSAKEIATRLDISGAKGVFTQDVVLRGGRALPLYSKV----VEAAPA 70
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 267 GVSVgLCLTYENQSAMKREDtkwqperDVWWQDVV-TKFPTKCDVE------WVDAEDPLFLLYTSGSTGKPKGV--LHT 337
Cdd:PLN03051 71 KAIV-LPAAGEPVAVPLREQ-------DLSWCDFLgVAAAQGSVGGneyspvYAPVESVTNILFSSGTTGEPKAIpwTHL 142
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 338 SGgymVYTATTFKYAFDYKPTDIYWCTADCGWITGhSYVTYGPLLNGATVLVFEGTPNYPDSGrcwDIVDKYNVTIFYTA 417
Cdd:PLN03051 143 SP---LRCASDGWAHMDIQPGDVVCWPTNLGWMMG-PWLLYSAFLNGATLALYGGAPLGRGFG---KFVQDAGVTVLGLV 215
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 418 PTLVRSLMRDGTEYVTRYSRKSLRVLGSVGEPINPSAWRWFYNIVGDSRcPISDTWWQTETGGFMI--TPLPgawPQKPG 495
Cdd:PLN03051 216 PSIVKAWRHTGAFAMEGLDWSKLRVFASTGEASAVDDVLWLSSVRGYYK-PVIEYCGGTELASGYIssTLLQ---PQAPG 291
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 496 SATFPFFGVQPVIVDEKGQEI--EGECSGYLCIKKSWPGAF-RTLYGDHERyetTYFKPFAGYYFT-------GDGCSRD 565
Cdd:PLN03051 292 AFSTASLGTRFVLLNDNGVPYpdDQPCVGEVALAPPMLGASdRLLNADHDK---VYYKGMPMYGSKgmplrrhGDIMKRT 368
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 566 KDGYHWLTGRVDDVINVSGHRIGTAEVESALVSHPQC-AEAAVVGVEHEVKG-QGIYAFVTLVD-----GVPYSEELRKS 638
Cdd:PLN03051 369 PGGYFCVQGRADDTMNLGGIKTSSVEIERACDRAVAGiAETAAVGVAPPDGGpELLVIFLVLGEekkgfDQARPEALQKK 448
|
490 500 510
....*....|....*....|....*....|....*...
gi 242061796 639 LIMTVRNQIGAFAAPDKIHWAPGLPKTRSGKIMRRILR 676
Cdd:PLN03051 449 FQEAIQTNLNPLFKVSRVKIVPELPRNASNKLLRRVLR 486
|
|
| ttLC_FACS_AlkK_like |
cd12119 |
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles; This family includes ... |
140-677 |
6.08e-35 |
|
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles; This family includes fatty acyl-CoA synthetases that can activate medium-chain to long-chain fatty acids. They catalyze the ATP-dependent acylation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. The fatty acyl-CoA synthetases are responsible for fatty acid degradation as well as physiological regulation of cellular functions via the production of fatty acyl-CoA esters. The fatty acyl-CoA synthetase from Thermus thermophiles in this family catalyzes the long-chain fatty acid, myristoyl acid, while another member in this family, the AlkK protein identified from Pseudomonas oleovorans, targets medium chain fatty acids. This family also includes uncharacterized FACS proteins.
Pssm-ID: 341284 [Multi-domain] Cd Length: 518 Bit Score: 139.69 E-value: 6.08e-35
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 140 ESGNGDKIAMYWEGNEPsqDGKLTYSELLEKVCQLANYLKSLGVGKGDAV-------IIYLPmllelpiAMLACARIGAV 212
Cdd:cd12119 6 ARLHGDREIVSRTHEGE--VHRYTYAEVAERARRLANALRRLGVKPGDRVatlawntHRHLE-------LYYAVPGMGAV 76
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 213 HSVVFAGFSADSLAQRIVDCKPKLVItcnavkrgVKPIFLKdIVDVALVESEKngVSVGLCLTYENQSAMKRedtkwqPE 292
Cdd:cd12119 77 LHTINPRLFPEQIAYIINHAEDRVVF--------VDRDFLP-LLEAIAPRLPT--VEHVVVMTDDAAMPEPA------GV 139
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 293 RDVWWQDVVTKFPTKCDVEWVDAEDPLFLLYTSGSTGKPKGV--------LHTSGGYMvytattfKYAFDYKPTDIY--- 361
Cdd:cd12119 140 GVLAYEELLAAESPEYDWPDFDENTAAAICYTSGTTGNPKGVvyshrslvLHAMAALL-------TDGLGLSESDVVlpv 212
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 362 --------WCTADCGWITGHSYVTYGPLLNGATVLvfegtpnypdsgrcwDIVDKYNVTIFYTAPTLVRSLMrdgtEYVT 433
Cdd:cd12119 213 vpmfhvnaWGLPYAAAMVGAKLVLPGPYLDPASLA---------------ELIEREGVTFAAGVPTVWQGLL----DHLE 273
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 434 R--YSRKSLRVLGSVGEPINPSAWRWF-YNIVgdsrcPISDTWWQTETG--GFMITPLPG----------AWPQKPGsat 498
Cdd:cd12119 274 AngRDLSSLRRVVIGGSAVPRSLIEAFeERGV-----RVIHAWGMTETSplGTVARPPSEhsnlsedeqlALRAKQG--- 345
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 499 FPFFGVQPVIVDEKGQEIE--GECSGYLCIKKSW-PGAFrtlYGDHERYEttyfKPFAGYYF-TGDGCSRDKDGYHWLTG 574
Cdd:cd12119 346 RPVPGVELRIVDDDGRELPwdGKAVGELQVRGPWvTKSY---YKNDEESE----ALTEDGWLrTGDVATIDEDGYLTITD 418
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 575 RVDDVINVSGHRIGTAEVESALVSHPQCAEAAVVGVEHEVKGQGIYAFVTLVDGV-PYSEELRKSLimtvRNQIGAFAAP 653
Cdd:cd12119 419 RSKDVIKSGGEWISSVELENAIMAHPAVAEAAVIGVPHPKWGERPLAVVVLKEGAtVTAEELLEFL----ADKVAKWWLP 494
|
570 580
....*....|....*....|....
gi 242061796 654 DKIHWAPGLPKTRSGKIMRRILRK 677
Cdd:cd12119 495 DDVVFVDEIPKTSTGKIDKKALRE 518
|
|
| PRK07798 |
PRK07798 |
acyl-CoA synthetase; Validated |
159-669 |
2.35e-34 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236100 [Multi-domain] Cd Length: 533 Bit Score: 138.09 E-value: 2.35e-34
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 159 DGKLTYSELLEKVCQLANYLKSLGVGKGDAVIIYLPMLLELPIAMLACARIGAVH------------------SVVFAGF 220
Cdd:PRK07798 26 DRRLTYAELEERANRLAHYLIAQGLGPGDHVGIYARNRIEYVEAMLGAFKARAVPvnvnyryvedelryllddSDAVALV 105
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 221 SADSLAQRIVDCKPKL--VITCNAVKRGVKPIFLKDIVDvalvesekngvsvglcltYEnqSAMKREDtkwqPERDvwwq 298
Cdd:PRK07798 106 YEREFAPRVAEVLPRLpkLRTLVVVEDGSGNDLLPGAVD------------------YE--DALAAGS----PERD---- 157
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 299 dvvtkFPTKCdvewvdaEDPLFLLYTSGSTGKPKGVL-------HTS-GGYMVYTATTfkyafdykPTDIYWCTADCGWI 370
Cdd:PRK07798 158 -----FGERS-------PDDLYLLYTGGTTGMPKGVMwrqedifRVLlGGRDFATGEP--------IEDEEELAKRAAAG 217
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 371 TG----------H---SYVTYGPLLNGATVlVFEGTPNYpDSGRCWDIVDKYNVT-IFYT----APTLVRSLMRDGteyv 432
Cdd:PRK07798 218 PGmrrfpapplmHgagQWAAFAALFSGQTV-VLLPDVRF-DADEVWRTIEREKVNvITIVgdamARPLLDALEARG---- 291
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 433 tRYSRKSLRVLGSVGEPINPS---AW-RWFYNIVgdsrcpISDTWWQTETGgFMITPLPGAWPQKPGSATFPFfGVQPVI 508
Cdd:PRK07798 292 -PYDLSSLFAIASGGALFSPSvkeALlELLPNVV------LTDSIGSSETG-FGGSGTVAKGAVHTGGPRFTI-GPRTVV 362
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 509 VDEKGQEIE--GECSGYLCIKKSWPGAFrtlYGDHERYETTyFKPFAG--YYFTGDGCSRDKDGYHWLTGRVDDVINVSG 584
Cdd:PRK07798 363 LDEDGNPVEpgSGEIGWIARRGHIPLGY---YKDPEKTAET-FPTIDGvrYAIPGDRARVEADGTITLLGRGSVCINTGG 438
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 585 HRIGTAEVESALVSHPQCAEAAVVGVEHEVKGQGIYAFVTLVDGVPYSEElrkSLIMTVRNQIGAFAAPDKIHWAPGLPK 664
Cdd:PRK07798 439 EKVFPEEVEEALKAHPDVADALVVGVPDERWGQEVVAVVQLREGARPDLA---ELRAHCRSSLAGYKVPRAIWFVDEVQR 515
|
....*
gi 242061796 665 TRSGK 669
Cdd:PRK07798 516 SPAGK 520
|
|
| DltA |
cd05945 |
D-alanine:D-alanyl carrier protein ligase (DltA) and similar proteins; This family includes ... |
158-675 |
4.01e-34 |
|
D-alanine:D-alanyl carrier protein ligase (DltA) and similar proteins; This family includes D-alanyl carrier protein ligase DltA and aliphatic beta-amino acid adenylation enzymes IdnL1 and CmiS6. DltA incorporates D-ala in techoic acids in gram-positive bacteria via a two-step process, starting with adenylation of D-alanine that transfers D-alanine to the D-alanyl carrier protein. IdnL1, a short-chain aliphatic beta-amino acid adenylation enzyme, recognizes 3-aminobutanoic acid, and is involved in the synthesis of the macrolactam antibiotic incednine. CmiS6 is a medium-chain beta-amino acid adenylation enzyme that recognizes 3-aminononanoic acid, and is involved in the synthesis of cremimycin, also a macrolactam antibiotic. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341267 [Multi-domain] Cd Length: 449 Bit Score: 135.84 E-value: 4.01e-34
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 158 QDGKLTYSELLEKVCQLANYLKSLGVGKGDAVIIYLPMLLELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPKLV 237
Cdd:cd05945 13 GGRTLTYRELKERADALAAALASLGLDAGDPVVVYGHKSPDAIAAFLAALKAGHAYVPLDASSPAERIREILDAAKPALL 92
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 238 ItcnavkrgvkpiflkdivdvalvesekngvsvglcltyenqsamkredtkwqperdvwwqdvvtkfptkcdvewVDAED 317
Cdd:cd05945 93 I--------------------------------------------------------------------------ADGDD 98
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 318 PLFLLYTSGSTGKPKGVLHTSGGYMVYT---------------ATTFKYAFDYKPTDIYwctadCGWITGHSYVTYGPLL 382
Cdd:cd05945 99 NAYIIFTSGSTGRPKGVQISHDNLVSFTnwmlsdfplgpgdvfLNQAPFSFDLSVMDLY-----PALASGATLVPVPRDA 173
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 383 NGATVLVFEGTPnypdsgrcwdivdKYNVTIFYTAPTLVRSLMRDGTeyVTRYSRKSLRVLGSVGEPI-NPSAWRWFyni 461
Cdd:cd05945 174 TADPKQLFRFLA-------------EHGITVWVSTPSFAAMCLLSPT--FTPESLPSLRHFLFCGEVLpHKTARALQ--- 235
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 462 vgdSR---CPISDTWWQTETggfMITPLPGAWPQKPGSAT------FPFFGVQPVIVDEKGQEIEGECSGYLCIkkSWPG 532
Cdd:cd05945 236 ---QRfpdARIYNTYGPTEA---TVAVTYIEVTPEVLDGYdrlpigYAKPGAKLVILDEDGRPVPPGEKGELVI--SGPS 307
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 533 AFRTLYGDHERYETTYFkPFAGY--YFTGDGCSRDKDGYHWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAAVVGV 610
Cdd:cd05945 308 VSKGYLNNPEKTAAAFF-PDEGQraYRTGDLVRLEADGLLFYRGRLDFQVKLNGYRIELEEIEAALRQVPGVKEAVVVPK 386
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 242061796 611 EHEVKGQGIYAFVTLVDGVPY--SEELRKSLimtvRNQIGAFAAPDKIHWAPGLPKTRSGKIMRRIL 675
Cdd:cd05945 387 YKGEKVTELIAFVVPKPGAEAglTKAIKAEL----AERLPPYMIPRRFVYLDELPLNANGKIDRKAL 449
|
|
| PRK08276 |
PRK08276 |
long-chain-fatty-acid--CoA ligase; Validated |
162-676 |
7.06e-34 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 236215 [Multi-domain] Cd Length: 502 Bit Score: 136.19 E-value: 7.06e-34
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 162 LTYSELLEKVCQLANYLKSLGVGKGDAVIIYLPMLLELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPKLVIT-- 239
Cdd:PRK08276 12 VTYGELEARSNRLAHGLRALGLREGDVVAILLENNPEFFEVYWAARRSGLYYTPINWHLTAAEIAYIVDDSGAKVLIVsa 91
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 240 -CNAVKRGVKPIFLKDIVDVALVESEKNGvsvglCLTYENQSAMkredtkwQPERDVwwqdvvtkfptkcDVEWVDAEdp 318
Cdd:PRK08276 92 aLADTAAELAAELPAGVPLLLVVAGPVPG-----FRSYEEALAA-------QPDTPI-------------ADETAGAD-- 144
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 319 lfLLYTSGSTGKPKGVL--------HTSGGYMvytATTFKYAFDYKPTDIYWCTADcgwitghSYVTyGPLLNGATVLVF 390
Cdd:PRK08276 145 --MLYSSGTTGRPKGIKrplpgldpDEAPGMM---LALLGFGMYGGPDSVYLSPAP-------LYHT-APLRFGMSALAL 211
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 391 EGT----PNYpDSGRCWDIVDKYNVTIFYTAPTLVRSLMRDGTEYVTRYSRKSLRVLGSVGEPinpsawrwfynivgdsr 466
Cdd:PRK08276 212 GGTvvvmEKF-DAEEALALIERYRVTHSQLVPTMFVRMLKLPEEVRARYDVSSLRVAIHAAAP----------------- 273
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 467 CPIS------DtWW---------QTETGGF-MITPlpGAWPQKPGSATFPFFGVQPvIVDEKGQEI----EG----ECSG 522
Cdd:PRK08276 274 CPVEvkramiD-WWgpiiheyyaSSEGGGVtVITS--EDWLAHPGSVGKAVLGEVR-ILDEDGNELppgeIGtvyfEMDG 349
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 523 YlcikkswpgAFrTLYGDHERYETTYFKpfAGYYFTGDGCSRDKDGYHWLTGRVDDVINVSGHRIGTAEVESALVSHPQC 602
Cdd:PRK08276 350 Y---------PF-EYHNDPEKTAAARNP--HGWVTVGDVGYLDEDGYLYLTDRKSDMIISGGVNIYPQEIENLLVTHPKV 417
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 242061796 603 AEAAVVGVEHEVKGQGIYAFVTLVDGVPYSEELRKSLIMTVRNQIGAFAAPDKIHWAPGLPKTRSGKIMRRILR 676
Cdd:PRK08276 418 ADVAVFGVPDEEMGERVKAVVQPADGADAGDALAAELIAWLRGRLAHYKCPRSIDFEDELPRTPTGKLYKRRLR 491
|
|
| LC_FACS_like |
cd05935 |
Putative long-chain fatty acid CoA ligase; The members of this family are putative long-chain ... |
162-675 |
7.80e-34 |
|
Putative long-chain fatty acid CoA ligase; The members of this family are putative long-chain fatty acyl-CoA synthetases, which catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. Fatty acyl-CoA synthetases are responsible for fatty acid degradation as well as physiological regulation of cellular functions via the production of fatty acyl-CoA esters.
Pssm-ID: 341258 [Multi-domain] Cd Length: 430 Bit Score: 134.53 E-value: 7.80e-34
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 162 LTYSELLEKVCQLANYLKSLGVGKGDAVIIYLPMLLELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPKLVITcn 241
Cdd:cd05935 2 LTYLELLEVVKKLASFLSNKGVRKGDRVGICLQNSPQYVIAYFAIWRANAVVVPINPMLKERELEYILNDSGAKVAVV-- 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 242 avkrgvkpifLKDIVDVALvesekngvsvglcltyenqsamkredtkwqperdvwwqdvvtkfptkcdvewvdaedplfL 321
Cdd:cd05935 80 ----------GSELDDLAL------------------------------------------------------------I 89
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 322 LYTSGSTGKPKGVLHTSGGyMVYTATTFKYAFDYKPTDIYWCTADCGWITGHSYVTYGPLLNGATVLVFEGTpnypDSGR 401
Cdd:cd05935 90 PYTSGTTGLPKGCMHTHFS-AAANALQSAVWTGLTPSDVILACLPLFHVTGFVGSLNTAVYVGGTYVLMARW----DRET 164
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 402 CWDIVDKYNVTIFYTAPTLVRSLMrdGTEYVTRYSRKSLRVLGSVGEPINPSAWRWFYNIVGdsrCPISDTWWQTETggf 481
Cdd:cd05935 165 ALELIEKYKVTFWTNIPTMLVDLL--ATPEFKTRDLSSLKVLTGGGAPMPPAVAEKLLKLTG---LRFVEGYGLTET--- 236
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 482 mITPLPGAWPQKPGSATF--PFFGVQPVIVD-EKGQEIEGECSGYLCIKKswPGAFRTlYGDHERYETTYFKPFAG--YY 556
Cdd:cd05935 237 -MSQTHTNPPLRPKLQCLgiP*FGVDARVIDiETGRELPPNEVGEIVVRG--PQIFKG-YWNRPEETEESFIEIKGrrFF 312
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 557 FTGDGCSRDKDGYHWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAAVVGVEHEVKGQGIYAFVTLvdgvpySEELR 636
Cdd:cd05935 313 RTGDLGYMDEEGYFFFVDRVKRMINVSGFKVWPAEVEAKLYKHPAI*EVCVISVPDERVGEEVKAFIVL------RPEYR 386
|
490 500 510 520
....*....|....*....|....*....|....*....|....
gi 242061796 637 -----KSLIMTVRNQIGAFAAPDKIHWAPGLPKTRSGKIMRRIL 675
Cdd:cd05935 387 gkvteEDIIEWAREQMAAYKYPREVEFVDELPRSASGKILWRLL 430
|
|
| A_NRPS_Cytc1-like |
cd17643 |
similar to adenylation domain of cytotrienin synthetase CytC1; This family of the adenylation ... |
145-675 |
3.57e-33 |
|
similar to adenylation domain of cytotrienin synthetase CytC1; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes Streptomyces sp. cytotrienin synthetase (CytC1), a relatively promiscuous adenylation enzyme that installs the aminoacyl moieties on the phosphopantetheinyl arm of the holo carrier protein CytC2. Also included are Streptomyces sp Thr1, involved in the biosynthesis of 4-chlorothreonine, Pseudomonas aeruginosa pyoverdine synthetase D (PvdD), involved in the biosynthesis of the siderophore pyoverdine and Pseudomonas syringae syringopeptin synthetase, where syringpeptin is a necrosis-inducing phytotoxin that functions as a virulence determinant in the plant-pathogen interaction. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341298 [Multi-domain] Cd Length: 450 Bit Score: 133.20 E-value: 3.57e-33
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 145 DKIAMYWEGNEpsqdgkLTYSELLEKVCQLANYLKSLGVGKGDAVIIYLPMLLELPIAMLACARIGAVHSVVFAGFSADS 224
Cdd:cd17643 2 EAVAVVDEDRR------LTYGELDARANRLARTLRAEGVGPGDRVALALPRSAELIVALLAILKAGGAYVPIDPAYPVER 75
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 225 LAQRIVDCKPKLVITcnavkrgvkpiflkdivdvalvesekngvsvglcltyenqsamkredtkwqperdvwwqdvvtkf 304
Cdd:cd17643 76 IAFILADSGPSLLLT----------------------------------------------------------------- 90
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 305 ptkcdvewvDAEDPLFLLYTSGSTGKPKGVLHTSGGYM-VYTATTfkYAFDYKPTDIywctadcgWITGHSYV------- 376
Cdd:cd17643 91 ---------DPDDLAYVIYTSGSTGRPKGVVVSHANVLaLFAATQ--RWFGFNEDDV--------WTLFHSYAfdfsvwe 151
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 377 TYGPLLNGAT-VLVFEGTPNYPDsgRCWDIVDKYNVTIFYTAPTLVRSLM----RDGTEyvtrysRKSLR--VLGsvGEP 449
Cdd:cd17643 152 IWGALLHGGRlVVVPYEVARSPE--DFARLLRDEGVTVLNQTPSAFYQLVeaadRDGRD------PLALRyvIFG--GEA 221
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 450 INPSAWRWFYNIVGDSRCPISDTWWQTETGGFM----ITP--LPGAWPQKPGsATFPFFGVQpvIVDEKGQEIEGECSGY 523
Cdd:cd17643 222 LEAAMLRPWAGRFGLDRPQLVNMYGITETTVHVtfrpLDAadLPAAAASPIG-RPLPGLRVY--VLDADGRPVPPGVVGE 298
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 524 LCIkkSWPGAFRtlyGDHERYETTYFK----PFAG----YYFTGDGCSRDKDGYHWLTGRVDDVINVSGHRIGTAEVESA 595
Cdd:cd17643 299 LYV--SGAGVAR---GYLGRPELTAERfvanPFGGpgsrMYRTGDLARRLPDGELEYLGRADEQVKIRGFRIELGEIEAA 373
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 596 LVSHPQCAEAAVVGVEHEVKGQGIYAFVTLVDGV-PYSEELRKSLimtvRNQIGAFAAPDKIHWAPGLPKTRSGKIMRRI 674
Cdd:cd17643 374 LATHPSVRDAAVIVREDEPGDTRLVAYVVADDGAaADIAELRALL----KELLPDYMVPARYVPLDALPLTVNGKLDRAA 449
|
.
gi 242061796 675 L 675
Cdd:cd17643 450 L 450
|
|
| CBAL |
cd05923 |
4-Chlorobenzoate-CoA ligase (CBAL); CBAL catalyzes the conversion of 4-chlorobenzoate (4-CB) ... |
156-675 |
4.64e-33 |
|
4-Chlorobenzoate-CoA ligase (CBAL); CBAL catalyzes the conversion of 4-chlorobenzoate (4-CB) to 4-chlorobenzoyl-coenzyme A (4-CB-CoA) by the two-step adenylation and thioester-forming reactions. 4-Chlorobenzoate (4-CBA) is an environmental pollutant derived from microbial breakdown of aromatic pollutants, such as polychlorinated biphenyls (PCBs), DDT, and certain herbicides. The 4-CBA degrading pathway converts 4-CBA to the metabolite 4-hydroxybezoate (4-HBA), allowing some soil-dwelling microbes to utilize 4-CBA as an alternate carbon source. This pathway consists of three chemical steps catalyzed by 4-CBA-CoA ligase, 4-CBA-CoA dehalogenase, and 4HBA-CoA thioesterase in sequential reactions.
Pssm-ID: 341247 [Multi-domain] Cd Length: 493 Bit Score: 133.40 E-value: 4.64e-33
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 156 PSQDGKLTYSELLEKVCQLANYLKSLGVGKGDAVIIYLPMLLELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPK 235
Cdd:cd05923 23 PARGLRLTYSELRARIEAVAARLHARGLRPGQRVAVVLPNSVEAVIALLALHRLGAVPALINPRLKAAELAELIERGEMT 102
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 236 LVITCNAvKRGVKPIFLkdivdvALVESEKNGVSVGLcltyenqsamkREDTKWQPerdvwwqdvVTKFPTKcdvewvDA 315
Cdd:cd05923 103 AAVIAVD-AQVMDAIFQ------SGVRVLALSDLVGL-----------GEPESAGP---------LIEDPPR------EP 149
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 316 EDPLFLLYTSGSTGKPKGVL---HTSGGYMVYTATTFKYAFDYKPTDIywctadcGWITGHSYVTYGPLLNGAtvLVFEG 392
Cdd:cd05923 150 EQPAFVFYTSGTTGLPKGAVipqRAAESRVLFMSTQAGLRHGRHNVVL-------GLMPLYHVIGFFAVLVAA--LALDG 220
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 393 T---PNYPDSGRCWDIVDKYNVTIFYTAPTLVRSLMrdGTEYVTRYSRKSLRVLGSVGEPINPSAWRWFYNIVGDsrcPI 469
Cdd:cd05923 221 TyvvVEEFDPADALKLIEQERVTSLFATPTHLDALA--AAAEFAGLKLSSLRHVTFAGATMPDAVLERVNQHLPG---EK 295
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 470 SDTWWQTETGGFMITPLPgawpqKPGSATFPFFG--VQPVIVDEKGQEI-----EGEcsgyLCIKKSWPGAFRtlyGDHE 542
Cdd:cd05923 296 VNIYGTTEAMNSLYMRDA-----RTGTEMRPGFFseVRIVRIGGSPDEAlangeEGE----LIVAAAADAAFT---GYLN 363
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 543 RYETTYFKPFAGYYFTGDGCSRDKDGYHWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAAVVGVEHEVKGQGIYAF 622
Cdd:cd05923 364 QPEATAKKLQDGWYRTGDVGYVDPSGDVRILGRVDDMIISGGENIHPSEIERVLSRHPGVTEVVVIGVADERWGQSVTAC 443
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|...
gi 242061796 623 VTLVDGVPYSEELRKSLIMTvrnQIGAFAAPDKIHWAPGLPKTRSGKIMRRIL 675
Cdd:cd05923 444 VVPREGTLSADELDQFCRAS---ELADFKRPRRYFFLDELPKNAMNKVLRRQL 493
|
|
| A_NRPS_AB3403-like |
cd17646 |
Peptide Synthetase; The adenylation (A) domain of NRPS recognizes a specific amino acid or ... |
144-675 |
9.04e-33 |
|
Peptide Synthetase; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341301 [Multi-domain] Cd Length: 488 Bit Score: 132.78 E-value: 9.04e-33
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 144 GDKIAMYWEGnepsqdGKLTYSELLEKVCQLANYLKSLGVGKGDAVIIYLPMLLELPIAMLACARIGAVHSVVFAGFSAD 223
Cdd:cd17646 12 PDAPAVVDEG------RTLTYRELDERANRLAHLLRARGVGPEDRVAVLLPRSADLVVALLAVLKAGAAYLPLDPGYPAD 85
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 224 SLAQRIVDCKPKLVITcnavkRGVKPIFLKDIVDVALVEsekngvsvglcltyenqsamkREDTKWQPERDVWwqdvvtk 303
Cdd:cd17646 86 RLAYMLADAGPAVVLT-----TADLAARLPAGGDVALLG---------------------DEALAAPPATPPL------- 132
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 304 fptkcdvEWVDAEDPLFLLYTSGSTGKPKGVLHTSGGyMVYTATTFKYAFDYKPTDIYWCTADCG-----WitghsyVTY 378
Cdd:cd17646 133 -------VPPRPDNLAYVIYTSGSTGRPKGVMVTHAG-IVNRLLWMQDEYPLGPGDRVLQKTPLSfdvsvW------ELF 198
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 379 GPLLNGATVLVFEgtP-NYPDSGRCWDIVDKYNVTIFYTAPTLVRSLMRDgteyVTRYSRKSLRVLGSVGEPINPSAWRW 457
Cdd:cd17646 199 WPLVAGARLVVAR--PgGHRDPAYLAALIREHGVTTCHFVPSMLRVFLAE----PAAGSCASLRRVFCSGEALPPELAAR 272
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 458 F--------YNIVGDSRCPISDTWWQTEtggfmitplpGAWPQKPGSATFPFFGVQPVIVDEKGQEIEGECSGYLCIkks 529
Cdd:cd17646 273 FlalpgaelHNLYGPTEAAIDVTHWPVR----------GPAETPSVPIGRPVPNTRLYVLDDALRPVPVGVPGELYL--- 339
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 530 wpGAFRTLYGDHERYETTY-------FKPFAGYYFTGDGCSRDKDGYHWLTGRVDDVINVSGHRIGTAEVESALVSHPQC 602
Cdd:cd17646 340 --GGVQLARGYLGRPALTAerfvpdpFGPGSRMYRTGDLARWRPDGALEFLGRSDDQVKIRGFRVEPGEIEAALAAHPAV 417
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 242061796 603 AEAAVVGVEHEVKGQGIYAFVTLVDG--VPYSEELRKSLimtvRNQIGAFAAPDKIHWAPGLPKTRSGKIMRRIL 675
Cdd:cd17646 418 THAVVVARAAPAGAARLVGYVVPAAGaaGPDTAALRAHL----AERLPEYMVPAAFVVLDALPLTANGKLDRAAL 488
|
|
| EntF |
COG1020 |
EntF, seryl-AMP synthase component of non-ribosomal peptide synthetase [Secondary metabolites ... |
159-639 |
1.97e-32 |
|
EntF, seryl-AMP synthase component of non-ribosomal peptide synthetase [Secondary metabolites biosynthesis, transport and catabolism];
Pssm-ID: 440643 [Multi-domain] Cd Length: 1329 Bit Score: 134.98 E-value: 1.97e-32
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 159 DGKLTYSELLEKVCQLANYLKSLGVGKGDAVIIYLPMLLELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPKLVI 238
Cdd:COG1020 499 DQSLTYAELNARANRLAHHLRALGVGPGDLVGVCLERSLEMVVALLAVLKAGAAYVPLDPAYPAERLAYMLEDAGARLVL 578
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 239 TCNAVKRGVkpiflkdivdvalvesEKNGVSVgLCLTYENQSAMKREDtkwqPERDvwwqdvvtkfptkcdvewVDAEDP 318
Cdd:COG1020 579 TQSALAARL----------------PELGVPV-LALDALALAAEPATN----PPVP------------------VTPDDL 619
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 319 LFLLYTSGSTGKPKGVLHTSGGyMVYTATTFKYAFDYKPTDIywctadCGWITGHS-----YVTYGPLLNGAT-VLVFEG 392
Cdd:COG1020 620 AYVIYTSGSTGRPKGVMVEHRA-LVNLLAWMQRRYGLGPGDR------VLQFASLSfdasvWEIFGALLSGATlVLAPPE 692
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 393 TPnyPDSGRCWDIVDKYNVTIFYTAPTLVRSLMRDGTEyvtrySRKSLRVLGSVGEPINPSAWRWFYNIVGDSRC----- 467
Cdd:COG1020 693 AR--RDPAALAELLARHRVTVLNLTPSLLRALLDAAPE-----ALPSLRLVLVGGEALPPELVRRWRARLPGARLvnlyg 765
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 468 PisdtwwqTETG----GFMITPLPGAWPQKP-GSatfPFFGVQPVIVDEKGQ--------E--IEGEC--SGYLcikksw 530
Cdd:COG1020 766 P-------TETTvdstYYEVTPPDADGGSVPiGR---PIANTRVYVLDAHLQpvpvgvpgElyIGGAGlaRGYL------ 829
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 531 pgafrtlyGDH----ERYETTYFkPFAG--YYFTGDGCSRDKDG---YHwltGRVDDVINVSGHRIGTAEVESALVSHPQ 601
Cdd:COG1020 830 --------NRPeltaERFVADPF-GFPGarLYRTGDLARWLPDGnleFL---GRADDQVKIRGFRIELGEIEAALLQHPG 897
|
490 500 510
....*....|....*....|....*....|....*...
gi 242061796 602 CAEAAVVGVEHEVKGQGIYAFVTLVDGVPYSEELRKSL 639
Cdd:COG1020 898 VREAVVVAREDAPGDKRLVAYVVPEAGAAAAAALLRLA 935
|
|
| caiC |
PRK08008 |
putative crotonobetaine/carnitine-CoA ligase; Validated |
144-676 |
1.52e-31 |
|
putative crotonobetaine/carnitine-CoA ligase; Validated
Pssm-ID: 181195 [Multi-domain] Cd Length: 517 Bit Score: 129.42 E-value: 1.52e-31
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 144 GDKIAMYWEgnepSQDGK---LTYSELLEKVCQLANYLKSLGVGKGDAVIIYLPMLLELPIAMLACARIGAVHSVVFAGF 220
Cdd:PRK08008 21 GHKTALIFE----SSGGVvrrYSYLELNEEINRTANLFYSLGIRKGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINARL 96
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 221 SADSLAQRIVDCKPKLVITCNAVK------RGVKPIFLKDIVDVALVESEKNGVSvglcltyeNQSAMKREdtkwQPerd 294
Cdd:PRK08008 97 LREESAWILQNSQASLLVTSAQFYpmyrqiQQEDATPLRHICLTRVALPADDGVS--------SFTQLKAQ----QP--- 161
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 295 vwwqdvvtkfPTKCDVEWVDAEDPLFLLYTSGSTGKPKGVLHTS-----GGYmvYTAttFKYAFDYKptDIYWCTADCGW 369
Cdd:PRK08008 162 ----------ATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHynlrfAGY--YSA--WQCALRDD--DVYLTVMPAFH 225
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 370 ITGHSYVTYGPLLNGATVLVFEgtpNYPDSgRCWDIVDKYNVTIFYTAPTLVRSLMrdgTEYVTRYSRK-SLRVLgsvge 448
Cdd:PRK08008 226 IDCQCTAAMAAFSAGATFVLLE---KYSAR-AFWGQVCKYRATITECIPMMIRTLM---VQPPSANDRQhCLREV----- 293
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 449 pinpsawrWFYnivgdsrCPISD----------------TWWQTETGGFMITPLPGA---WPqkpgSATFPFFGVQPVIV 509
Cdd:PRK08008 294 --------MFY-------LNLSDqekdafeerfgvrlltSYGMTETIVGIIGDRPGDkrrWP----SIGRPGFCYEAEIR 354
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 510 DEKGQEIEGECSGYLCIKkSWPGA--FRTLYGDHERYETTyFKPfAGYYFTGDGCSRDKDGYHWLTGRVDDVINVSGHRI 587
Cdd:PRK08008 355 DDHNRPLPAGEIGEICIK-GVPGKtiFKEYYLDPKATAKV-LEA-DGWLHTGDTGYVDEEGFFYFVDRRCNMIKRGGENV 431
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 588 GTAEVESALVSHPQCAEAAVVGVEHEVKGQGIYAFVTLVDGVPYSEElrkSLIMTVRNQIGAFAAPDKIHWAPGLPKTRS 667
Cdd:PRK08008 432 SCVELENIIATHPKIQDIVVVGIKDSIRDEAIKAFVVLNEGETLSEE---EFFAFCEQNMAKFKVPSYLEIRKDLPRNCS 508
|
....*....
gi 242061796 668 GKIMRRILR 676
Cdd:PRK08008 509 GKIIKKNLK 517
|
|
| PRK13295 |
PRK13295 |
cyclohexanecarboxylate-CoA ligase; Reviewed |
161-680 |
1.63e-31 |
|
cyclohexanecarboxylate-CoA ligase; Reviewed
Pssm-ID: 171961 [Multi-domain] Cd Length: 547 Bit Score: 129.79 E-value: 1.63e-31
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 161 KLTYSELLEKVCQLANYLKSLGVGKGDAVIIYLPMLLELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPKLVITC 240
Cdd:PRK13295 55 RFTYRELAALVDRVAVGLARLGVGRGDVVSCQLPNWWEFTVLYLACSRIGAVLNPLMPIFRERELSFMLKHAESKVLVVP 134
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 241 N--------AVKRGVKPIfLKDIVDVALVESE-KNGVSVGLCltyenqsamkreDTKWQPERDVwwqdvvtkfPTKCDVE 311
Cdd:PRK13295 135 KtfrgfdhaAMARRLRPE-LPALRHVVVVGGDgADSFEALLI------------TPAWEQEPDA---------PAILARL 192
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 312 WVDAEDPLFLLYTSGSTGKPKGVLHTSGGYMvytATTFKYA--FDYKPTDIYWCTADCGWITGHSYVTYGPLLNGATVLV 389
Cdd:PRK13295 193 RPGPDDVTQLIYTSGTTGEPKGVMHTANTLM---ANIVPYAerLGLGADDVILMASPMAHQTGFMYGLMMPVMLGATAVL 269
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 390 FEgtpnYPDSGRCWDIVDKYNVTiFYTAPTlvrSLMRDGTEYV--TRYSRKSLRVLGSVGEPINPSAWRWFYNIVGDSRC 467
Cdd:PRK13295 270 QD----IWDPARAAELIRTEGVT-FTMAST---PFLTDLTRAVkeSGRPVSSLRTFLCAGAPIPGALVERARAALGAKIV 341
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 468 PisdTWWQTETGGFMITpLPGAWPQKpGSAT--FPFFGVQPVIVDEKGQEIEGECSGYLCIKKswPGAFRTLYGDHERYE 545
Cdd:PRK13295 342 S---AWGMTENGAVTLT-KLDDPDER-ASTTdgCPLPGVEVRVVDADGAPLPAGQIGRLQVRG--CSNFGGYLKRPQLNG 414
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 546 TTyfkpFAGYYFTGDGCSRDKDGYHWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAAVVGVEHEVKGQGIYAFVTL 625
Cdd:PRK13295 415 TD----ADGWFDTGDLARIDADGYIRISGRSKDVIIRGGENIPVVEIEALLYRHPAIAQVAIVAYPDERLGERACAFVVP 490
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*.
gi 242061796 626 VDGVPYS-EELRKSLimtVRNQIGAFAAPDKIHWAPGLPKTRSGKIMRRILRKIAA 680
Cdd:PRK13295 491 RPGQSLDfEEMVEFL---KAQKVAKQYIPERLVVRDALPRTPSGKIQKFRLREMLR 543
|
|
| A_NRPS_PvdJ-like |
cd17649 |
non-ribosomal peptide synthetase; This family of the adenylation (A) domain of nonribosomal ... |
159-676 |
2.83e-31 |
|
non-ribosomal peptide synthetase; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes pyoverdine biosynthesis protein PvdJ involved in the synthesis of pyoverdine, which consists of a chromophore group attached to a variable peptide chain and comprises around 6-12 amino acids that are specific for each Pseudomonas species, and for which the peptide might be first synthesized before the chromophore assembly. Also included is ornibactin biosynthesis protein OrbI; ornibactin is a tetrapeptide siderophore with an l-ornithine-d-hydroxyaspartate-l-serine-l-ornithine backbone. The adenylation domain at the N-terminal of OrbI possibly initiates the ornibactin with the binding of N5-hydroxyornithine. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341304 [Multi-domain] Cd Length: 450 Bit Score: 127.48 E-value: 2.83e-31
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 159 DGKLTYSELLEKVCQLANYLKSLGVGKGDAVIIYLPMLLELPIAMLACARIGAVhsvvFAGFSADSLAQRIvdckpklvi 238
Cdd:cd17649 10 DQSLSYAELDARANRLAHRLRALGVGPEVRVGIALERSLEMVVALLAILKAGGA----YVPLDPEYPAERL--------- 76
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 239 tcnavkrgvkpiflkdivDVALVESEkngvsVGLCLTYENQSamkredtkwqperdvwwqdvvtkfptkcdvewvdaedP 318
Cdd:cd17649 77 ------------------RYMLEDSG-----AGLLLTHHPRQ-------------------------------------L 96
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 319 LFLLYTSGSTGKPKGVLHTSGGYMVYTATTFKYaFDYKPTDIYWCTADCGWITGHSYVtYGPLLNGATVLVfEGTPNYPD 398
Cdd:cd17649 97 AYVIYTSGSTGTPKGVAVSHGPLAAHCQATAER-YGLTPGDRELQFASFNFDGAHEQL-LPPLICGACVVL-RPDELWAS 173
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 399 SGRCWDIVDKYNVTIFYTAPTLVRSLMRDgTEYVTRYSRKSLRVLGSVGEPINPS-AWRWfynivgdsrCPISDTWWQ-- 475
Cdd:cd17649 174 ADELAEMVRELGVTVLDLPPAYLQQLAEE-ADRTGDGRPPSLRLYIFGGEALSPElLRRW---------LKAPVRLFNay 243
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 476 --TETggfMITPLpgAWPQKPGSATF--------PFFGVQPVIVDEKGQEIEGECSGYLCIkkSWPGAFRtlyGDHERYE 545
Cdd:cd17649 244 gpTEA---TVTPL--VWKCEAGAARAgasmpigrPLGGRSAYILDADLNPVPVGVTGELYI--GGEGLAR---GYLGRPE 313
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 546 TT--------YFKPFAGYYFTGDGCSRDKDGYHWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAAVVGVEHEVKGQ 617
Cdd:cd17649 314 LTaerfvpdpFGAPGSRLYRTGDLARWRDDGVIEYLGRVDHQVKIRGFRIELGEIEAALLEHPGVREAAVVALDGAGGKQ 393
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*....
gi 242061796 618 gIYAFVTLVDGVPySEELRKSLIMTVRNQIGAFAAPDKIHWAPGLPKTRSGKIMRRILR 676
Cdd:cd17649 394 -LVAYVVLRAAAA-QPELRAQLRTALRASLPDYMVPAHLVFLARLPLTPNGKLDRKALP 450
|
|
| PRK09088 |
PRK09088 |
acyl-CoA synthetase; Validated |
163-676 |
4.80e-31 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 181644 [Multi-domain] Cd Length: 488 Bit Score: 127.62 E-value: 4.80e-31
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 163 TYSELLEKVCQLANYLKSLGVGKGDAVIIYLPMLLELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPKLVITCNA 242
Cdd:PRK09088 24 TYAELDALVGRLAAVLRRRGCVDGERLAVLARNSVWLVALHFACARVGAIYVPLNWRLSASELDALLQDAEPRLLLGDDA 103
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 243 VKRGVKpiflkDIVDVALVESEKNGVsvglcltyenqsamkredtkwqperdvwwqdvvtkfpTKCDVEWVDAEDPLFLL 322
Cdd:PRK09088 104 VAAGRT-----DVEDLAAFIASADAL-------------------------------------EPADTPSIPPERVSLIL 141
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 323 YTSGSTGKPKGVLhTSGGYMVYTATTFKYAFDYKPTDIYWCTADCGWITGHSYVTYGPLLNGATVLVFEGTPNYPDSGRC 402
Cdd:PRK09088 142 FTSGTSGQPKGVM-LSERNLQQTAHNFGVLGRVDAHSSFLCDAPMFHIIGLITSVRPVLAVGGSILVSNGFEPKRTLGRL 220
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 403 WDIvdKYNVTIFYTAPTLVRSLMRDGTEYVTRYSRksLRVLGSVGEPINPSAWRWFYnivgDSRCPISDTWWQTETGGFM 482
Cdd:PRK09088 221 GDP--ALGITHYFCVPQMAQAFRAQPGFDAAALRH--LTALFTGGAPHAAEDILGWL----DDGIPMVDGFGMSEAGTVF 292
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 483 ITPL-PGAWPQKPGSATFPFFGVQPVIVDEKGQEIEGECSGYLCIKKswPGAFRTLYGDHEryETTYFKPFAGYYFTGDG 561
Cdd:PRK09088 293 GMSVdCDVIRAKAGAAGIPTPTVQTRVVDDQGNDCPAGVPGELLLRG--PNLSPGYWRRPQ--ATARAFTGDGWFRTGDI 368
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 562 CSRDKDGYHWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAAVVGVEHEVKGQGIYAFVTLVDGVPYS-EELRKSLI 640
Cdd:PRK09088 369 ARRDADGFFWVVDRKKDMFISGGENVYPAEIEAVLADHPGIRECAVVGMADAQWGEVGYLAIVPADGAPLDlERIRSHLS 448
|
490 500 510
....*....|....*....|....*....|....*.
gi 242061796 641 mtvrNQIGAFAAPDKIHWAPGLPKTRSGKIMRRILR 676
Cdd:PRK09088 449 ----TRLAKYKVPKHLRLVDALPRTASGKLQKARLR 480
|
|
| PRK13382 |
PRK13382 |
bile acid CoA ligase; |
160-676 |
9.21e-31 |
|
bile acid CoA ligase;
Pssm-ID: 172019 [Multi-domain] Cd Length: 537 Bit Score: 127.18 E-value: 9.21e-31
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 160 GKLTYSELLEKVCQLANYLKSLGVGKGDAVIIYLPMLLELPIAMLACARIGAvHSVVF-AGFSADSLAQRIVDCKPKLVI 238
Cdd:PRK13382 67 GTLTWRELDERSDALAAALQALPIGEPRVVGIMCRNHRGFVEALLAANRIGA-DILLLnTSFAGPALAEVVTREGVDTVI 145
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 239 tcnaVKRGVKPIFLKDIVD-------VALVESEKNGVSVGLCLTYENQsamkredtkwQPERDvwwqdvvtkfPTKCDVe 311
Cdd:PRK13382 146 ----YDEEFSATVDRALADcpqatriVAWTDEDHDLTVEVLIAAHAGQ----------RPEPT----------GRKGRV- 200
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 312 wvdaedplfLLYTSGSTGKPKGVLHT-SGGYMvytatTFKYAFDYKPtdiywctadcgWITGHSYVTYGPLLN--GATVL 388
Cdd:PRK13382 201 ---------ILLTSGTTGTPKGARRSgPGGIG-----TLKAILDRTP-----------WRAEEPTVIVAPMFHawGFSQL 255
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 389 VFEGTPNYP-------DSGRCWDIVDKYNVTIFYTAPTLVRSLMRDGTEYVTRYSRKSLRVLGSVGEPINPSAWRWFYNI 461
Cdd:PRK13382 256 VLAASLACTivtrrrfDPEATLDLIDRHRATGLAVVPVMFDRIMDLPAEVRNRYSGRSLRFAAASGSRMRPDVVIAFMDQ 335
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 462 VGDSrcpISDTWWQTETGgfMI-TPLPGAWPQKPGSATFPFFGVQPVIVDEKGQEI-EGECsGYLCIKKSwpgafrTLYg 539
Cdd:PRK13382 336 FGDV---IYNNYNATEAG--MIaTATPADLRAAPDTAGRPAEGTEIRILDQDFREVpTGEV-GTIFVRND------TQF- 402
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 540 dhERYETTYFKPF-AGYYFTGDGCSRDKDGYHWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAAVVGVEHEVKGQG 618
Cdd:PRK13382 403 --DGYTSGSTKDFhDGFMASGDVGYLDENGRLFVVGRDDEMIVSGGENVYPIEVEKTLATHPDVAEAAVIGVDDEQYGQR 480
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*...
gi 242061796 619 IYAFVTLVDGVPYSEELRKSlimTVRNQIGAFAAPDKIHWAPGLPKTRSGKIMRRILR 676
Cdd:PRK13382 481 LAAFVVLKPGASATPETLKQ---HVRDNLANYKVPRDIVVLDELPRGATGKILRRELQ 535
|
|
| A_NRPS_TlmIV_like |
cd12114 |
The adenylation domain of nonribosomal peptide synthetases (NRPS), including ... |
159-675 |
1.23e-30 |
|
The adenylation domain of nonribosomal peptide synthetases (NRPS), including Streptoalloteichus tallysomycin biosynthesis genes; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions. This family includes the TLM biosynthetic gene cluster from Streptoalloteichus that consists of nine NRPS genes; the N-terminal module of TlmVI (NRPS-5) and the starter module of BlmVI (NRPS-5) are comprised of the acyl CoA ligase (AL) and acyl carrier protein (ACP)-like domains, which are thought to be involved in the biosynthesis of the beta-aminoalaninamide moiety.
Pssm-ID: 341279 [Multi-domain] Cd Length: 477 Bit Score: 126.23 E-value: 1.23e-30
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 159 DGKLTYSELLEKVCQLANYLKSLGVGKGDAVIIYLPMLLELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPKLVI 238
Cdd:cd12114 10 DGTLTYGELAERARRVAGALKAAGVRPGDLVAVTLPKGPEQVVAVLGILAAGAAYVPVDIDQPAARREAILADAGARLVL 89
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 239 TCNAVKRGVKPIFLKDIVDVALVESEKNGVSVGlcltyenqsamkredtkwqperdvwwqdvvtkfptkcdvewVDAEDP 318
Cdd:cd12114 90 TDGPDAQLDVAVFDVLILDLDALAAPAPPPPVD-----------------------------------------VAPDDL 128
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 319 LFLLYTSGSTGKPKGVLHTSGGYM-VYTATTFKYAFDykPTDIYWCTADCGwitgHSYVTY---GPLLNGAT-VLVFEGT 393
Cdd:cd12114 129 AYVIFTSGSTGTPKGVMISHRAALnTILDINRRFAVG--PDDRVLALSSLS----FDLSVYdifGALSAGATlVLPDEAR 202
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 394 PNYPDSgrcW-DIVDKYNVTIFYTAPTLVRSLMRDGTEYVTRYSrkSLR-VLGSvGEPIN---PSAWRwfyNIVGDSRcP 468
Cdd:cd12114 203 RRDPAH---WaELIERHGVTLWNSVPALLEMLLDVLEAAQALLP--SLRlVLLS-GDWIPldlPARLR---ALAPDAR-L 272
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 469 IS------DTWWQTEtggFMITPLPGAWPQKP-GsatFPFFGVQPVIVDEKGQEIEGECSGYLCIkkSWPGAFRTLYGDH 541
Cdd:cd12114 273 ISlggateASIWSIY---HPIDEVPPDWRSIPyG---RPLANQRYRVLDPRGRDCPDWVPGELWI--GGRGVALGYLGDP 344
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 542 ERYETTYFKPFAG--YYFTGD-GCSRDkDGYHWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAAVVGVEhEVKGQG 618
Cdd:cd12114 345 ELTAARFVTHPDGerLYRTGDlGRYRP-DGTLEFLGRRDGQVKVRGYRIELGEIEAALQAHPGVARAVVVVLG-DPGGKR 422
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*....
gi 242061796 619 IYAFVTLVDGVP--YSEELRKSLIMTVRnqigAFAAPDKIHWAPGLPKTRSGKIMRRIL 675
Cdd:cd12114 423 LAAFVVPDNDGTpiAPDALRAFLAQTLP----AYMIPSRVIALEALPLTANGKVDRAAL 477
|
|
| FACL_like_6 |
cd05922 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ... |
171-676 |
1.43e-30 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341246 [Multi-domain] Cd Length: 457 Bit Score: 125.63 E-value: 1.43e-30
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 171 VCQLANYLKSLGVGKGDAVIIYLPMLLELPIAMLACARIGAVHSVVFAGFSADSLAQ--RIV--DCKPKLVITCNAvkrg 246
Cdd:cd05922 3 VSAAASALLEAGGVRGERVVLILPNRFTYIELSFAVAYAGGRLGLVFVPLNPTLKESvlRYLvaDAGGRIVLADAG---- 78
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 247 vkpifLKDIVDVALVESEKNGVSVglcltyenqsamkREDTKWQPERDVwwqdvvTKFPtkcdvewVDAEDPLFLLYTSG 326
Cdd:cd05922 79 -----AADRLRDALPASPDPGTVL-------------DADGIRAARASA------PAHE-------VSHEDLALLLYTSG 127
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 327 STGKPKGVL--HTSggyMVYTATTFKYAFDYKPTDIYWCTADCGWITGHSYVTYGpLLNGATvLVFEGTPNYPDSgrCWD 404
Cdd:cd05922 128 STGSPKLVRlsHQN---LLANARSIAEYLGITADDRALTVLPLSYDYGLSVLNTH-LLRGAT-LVLTNDGVLDDA--FWE 200
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 405 IVDKYNVTIFYTAPT---LVRSLMRDGTEYvtrysrKSLRVLGSVGEPINPSAWRWFYNIVGDSRcpISDTWWQTETGGF 481
Cdd:cd05922 201 DLREHGATGLAGVPStyaMLTRLGFDPAKL------PSLRYLTQAGGRLPQETIARLRELLPGAQ--VYVMYGQTEATRR 272
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 482 MITPLPGAWPQKPGSATFPFFGVQPVIVDEKGQEIEGECSGYLCIKkswpGAFRTL-YGDHERYETtyfKP--FAGYYFT 558
Cdd:cd05922 273 MTYLPPERILEKPGSIGLAIPGGEFEILDDDGTPTPPGEPGEIVHR----GPNVMKgYWNDPPYRR---KEgrGGGVLHT 345
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 559 GDGCSRDKDGYHWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAAVVGVEHEVkGQGIYAFVTLVDGVPYSEELRKs 638
Cdd:cd05922 346 GDLARRDEDGFLFIVGRRDRMIKLFGNRISPTEIEAAARSIGLIIEAAAVGLPDPL-GEKLALFVTAPDKIDPKDVLRS- 423
|
490 500 510
....*....|....*....|....*....|....*...
gi 242061796 639 limtVRNQIGAFAAPDKIHWAPGLPKTRSGKIMRRILR 676
Cdd:cd05922 424 ----LAERLPPYKVPATVRVVDELPLTASGKVDYAALR 457
|
|
| PRK06188 |
PRK06188 |
acyl-CoA synthetase; Validated |
133-676 |
1.56e-30 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 235731 [Multi-domain] Cd Length: 524 Bit Score: 126.25 E-value: 1.56e-30
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 133 NAVDRNiesgnGDKIAMYWEgnepsqDGKLTYSELLEKVCQLANYLKSLGVGKGDAVIIYLPMLLELPIAMLACARIGAV 212
Cdd:PRK06188 20 SALKRY-----PDRPALVLG------DTRLTYGQLADRISRYIQAFEALGLGTGDAVALLSLNRPEVLMAIGAAQLAGLR 88
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 213 HSVVFAGFSADSLAQRIVDCKPK-LVITCNA-VKRGVkpiflkdiVDVALVESEKNGVSVGLCltyenqsamkredtkwq 290
Cdd:PRK06188 89 RTALHPLGSLDDHAYVLEDAGIStLIVDPAPfVERAL--------ALLARVPSLKHVLTLGPV----------------- 143
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 291 PE-RDVWwqDVVTKFPTKCDVEWVDAEDPLFLLYTSGSTGKPKGVLHTSGGYMVYTAttfkyafdykptdiyWCTADCGW 369
Cdd:PRK06188 144 PDgVDLL--AAAAKFGPAPLVAAALPPDIAGLAYTGGTTGKPKGVMGTHRSIATMAQ---------------IQLAEWEW 206
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 370 ITGHSYVTYGP------------LLNGATVLVFEGTpnypDSGRCWDIVDKYNVTIFYTAPTLVRSLMrDGTEYVTRySR 437
Cdd:PRK06188 207 PADPRFLMCTPlshaggafflptLLRGGTVIVLAKF----DPAEVLRAIEEQRITATFLVPTMIYALL-DHPDLRTR-DL 280
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 438 KSLRVLGSVGEPINPSAWRWFYNIVGdsrcPI-SDTWWQTETGGFmITPLP-----GAWPQKPGSATFPFFGVQPVIVDE 511
Cdd:PRK06188 281 SSLETVYYGASPMSPVRLAEAIERFG----PIfAQYYGQTEAPMV-ITYLRkrdhdPDDPKRLTSCGRPTPGLRVALLDE 355
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 512 KGQEIE-GEcSGYLCIKKswPGafrTLYGDHERYETTYfKPFA-GYYFTGDGCSRDKDGYHWLTGRVDDVINVSGHRIGT 589
Cdd:PRK06188 356 DGREVAqGE-VGEICVRG--PL---VMDGYWNRPEETA-EAFRdGWLHTGDVAREDEDGFYYIVDRKKDMIVTGGFNVFP 428
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 590 AEVESALVSHPQCAEAAVVGVEHEVKGQGIYAFVTLVDGVPYSEElrkSLIMTVRNQIGAFAAPDKIHWAPGLPKTRSGK 669
Cdd:PRK06188 429 REVEDVLAEHPAVAQVAVIGVPDEKWGEAVTAVVVLRPGAAVDAA---ELQAHVKERKGSVHAPKQVDFVDSLPLTALGK 505
|
....*..
gi 242061796 670 IMRRILR 676
Cdd:PRK06188 506 PDKKALR 512
|
|
| FACL_like_4 |
cd05944 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ... |
323-682 |
1.61e-30 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341266 [Multi-domain] Cd Length: 359 Bit Score: 123.36 E-value: 1.61e-30
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 323 YTSGSTGKPKGVLHTSGGyMVYTATTFKYAFDYKPTDIYWCTADCGWITGhSYVTYG-PLLNGATVlVFEGTPNYPDSG- 400
Cdd:cd05944 9 HTGGTTGTPKLAQHTHSN-EVYNAWMLALNSLFDPDDVLLCGLPLFHVNG-SVVTLLtPLASGAHV-VLAGPAGYRNPGl 85
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 401 --RCWDIVDKYNVTIFYTAPTLVRSLM-RDGTEYVTrysrkSLRVLGSVGEPINPSAWRWFYNIVGdsrCPISDTWWQTE 477
Cdd:cd05944 86 fdNFWKLVERYRITSLSTVPTVYAALLqVPVNADIS-----SLRFAMSGAAPLPVELRARFEDATG---LPVVEGYGLTE 157
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 478 TGGFMITPLPGAwPQKPGSA--TFPFFGVQPVIVDEKGQ---EIEGECSGYLCIKKswPGAFR-TLYGDHERyettyfKP 551
Cdd:cd05944 158 ATCLVAVNPPDG-PKRPGSVglRLPYARVRIKVLDGVGRllrDCAPDEVGEICVAG--PGVFGgYLYTEGNK------NA 228
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 552 FA--GYYFTGDGCSRDKDGYHWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAAVVGVEHEVKGQGIYAFVTLVDGV 629
Cdd:cd05944 229 FVadGWLNTGDLGRLDADGYLFITGRAKDLIIRGGHNIDPALIEEALLRHPAVAFAGAVGQPDAHAGELPVAYVQLKPGA 308
|
330 340 350 360 370
....*....|....*....|....*....|....*....|....*....|....
gi 242061796 630 PYSEElrkSLIMTVRNQIGAFAA-PDKIHWAPGLPKTRSGKIMRRILRKIAARQ 682
Cdd:cd05944 309 VVEEE---ELLAWARDHVPERAAvPKHIEVLEELPVTAVGKVFKPALRADAIHR 359
|
|
| PRK06155 |
PRK06155 |
crotonobetaine/carnitine-CoA ligase; Provisional |
162-678 |
4.48e-30 |
|
crotonobetaine/carnitine-CoA ligase; Provisional
Pssm-ID: 235719 [Multi-domain] Cd Length: 542 Bit Score: 125.26 E-value: 4.48e-30
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 162 LTYSELLEKVCQLANYLKSLGVGKGDAVIIYLPMLLELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPKLVItcn 241
Cdd:PRK06155 47 WTYAEAARAAAAAAHALAAAGVKRGDRVALMCGNRIEFLDVFLGCAWLGAIAVPINTALRGPQLEHILRNSGARLLV--- 123
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 242 avkrgvkpiflkdiVDVALVEsekngvsvglcltyenqsAMKREDTKWQPERDVWWQD--VVTKFPTKCDV-------EW 312
Cdd:PRK06155 124 --------------VEAALLA------------------ALEAADPGDLPLPAVWLLDapASVSVPAGWSTaplppldAP 171
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 313 VDAE-----DPLFLLYTSGSTGKPKGVLHTSGGYmvytattfkyafdykptdiYW----CTADCGWITGHSYVTYGPL-- 381
Cdd:PRK06155 172 APAAavqpgDTAAILYTSGTTGPSKGVCCPHAQF-------------------YWwgrnSAEDLEIGADDVLYTTLPLfh 232
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 382 -----------LNGATVLVfegTPNYPDSGrCWDIVDKYNVTIFYTAPTLVRSLMRDGTEYVTRYSRksLRVLGSVGEPi 450
Cdd:PRK06155 233 tnalnaffqalLAGATYVL---EPRFSASG-FWPAVRRHGATVTYLLGAMVSILLSQPARESDRAHR--VRVALGPGVP- 305
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 451 nPSAWRWFYNIVGdsrCPISDTWWQTETGGFMITPLPGawpQKPGSATFPFFGVQPVIVDEKGQEIEGECSGYLCIKKSW 530
Cdd:PRK06155 306 -AALHAAFRERFG---VDLLDGYGSTETNFVIAVTHGS---QRPGSMGRLAPGFEARVVDEHDQELPDGEPGELLLRADE 378
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 531 PGAFRTLY-GDHEryETTyfKPFAGYYF-TGDGCSRDKDGYHWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAAVV 608
Cdd:PRK06155 379 PFAFATGYfGMPE--KTV--EAWRNLWFhTGDRVVRDADGWFRFVDRIKDAIRRRGENISSFEVEQVLLSHPAVAAAAVF 454
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 609 GVEHEVKGQGIYAFVTLVDGVPYSEElrkSLIMTVRNQIGAFAAPDKIHWAPGLPKTRSGKIMRRILRKI 678
Cdd:PRK06155 455 PVPSELGEDEVMAAVVLRDGTALEPV---ALVRHCEPRLAYFAVPRYVEFVAALPKTENGKVQKFVLREQ 521
|
|
| ttLC_FACS_AEE21_like |
cd12118 |
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles and Arabidopsis; This ... |
161-677 |
8.23e-30 |
|
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles and Arabidopsis; This family includes fatty acyl-CoA synthetases that can activate medium to long-chain fatty acids. These enzymes catalyze the ATP-dependent acylation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. Fatty acyl-CoA synthetases are responsible for fatty acid degradation as well as physiological regulation of cellular functions via the production of fatty acyl-CoA esters. The fatty acyl-CoA synthetase from Thermus thermophiles in this family has been shown to catalyze the long-chain fatty acid, myristoyl acid. Also included in this family are acyl activating enzymes from Arabidopsis, which contains a large number of proteins from this family with up to 63 different genes, many of which are uncharacterized.
Pssm-ID: 341283 [Multi-domain] Cd Length: 486 Bit Score: 123.56 E-value: 8.23e-30
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 161 KLTYSELLEKVCQLANYLKSLGVGKGDAVIIYL---PMLLELPIAMLACariGAVHSVVFAGFSADSLAQRIVDCKPKLV 237
Cdd:cd12118 29 RYTWRQTYDRCRRLASALAALGISRGDTVAVLApntPAMYELHFGVPMA---GAVLNALNTRLDAEEIAFILRHSEAKVL 105
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 238 ItcnavkrgvkpiflkdiVDVALvesekngvsvglclTYENQSAMKREDTKWQPERDvwwqdvvtkfptkcdvEWvdaeD 317
Cdd:cd12118 106 F-----------------VDREF--------------EYEDLLAEGDPDFEWIPPAD----------------EW----D 134
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 318 PLFLLYTSGSTGKPKGVLHTSGGYMVyTATTFKYAFDYKPTDIYWCTAD----CGWItghsyVTYGPLLNGATVLVFEGT 393
Cdd:cd12118 135 PIALNYTSGTTGRPKGVVYHHRGAYL-NALANILEWEMKQHPVYLWTLPmfhcNGWC-----FPWTVAAVGGTNVCLRKV 208
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 394 pnypDSGRCWDIVDKYNVTIFYTAPTlVRSLMRDGTEYVTRYSRKSLRVLgSVGEPINPSAWRWFYNIVGDsrcpISDTW 473
Cdd:cd12118 209 ----DAKAIYDLIEKHKVTHFCGAPT-VLNMLANAPPSDARPLPHRVHVM-TAGAPPPAAVLAKMEELGFD----VTHVY 278
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 474 WQTETGGFMITplpGAWpqKPGSATFP--------------FFGVQPVIVDEK---------GQEIeGEC--------SG 522
Cdd:cd12118 279 GLTETYGPATV---CAW--KPEWDELPteerarlkarqgvrYVGLEEVDVLDPetmkpvprdGKTI-GEIvfrgnivmKG 352
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 523 YLCIKKSWPGAFRtlygdheryettyfkpfAGYYFTGDGCSRDKDGYHWLTGRVDDVINVSGHRIGTAEVESALVSHPQC 602
Cdd:cd12118 353 YLKNPEATAEAFR-----------------GGWFHSGDLAVIHPDGYIEIKDRSKDIIISGGENISSVEVEGVLYKHPAV 415
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 242061796 603 AEAAVVGVEHEVKGQGIYAFVTLVDGVPYSEElrkSLIMTVRNQIGAFAAPDKIHWAPgLPKTRSGKIMRRILRK 677
Cdd:cd12118 416 LEAAVVARPDEKWGEVPCAFVELKEGAKVTEE---EIIAFCREHLAGFMVPKTVVFGE-LPKTSTGKIQKFVLRD 486
|
|
| PRK07470 |
PRK07470 |
acyl-CoA synthetase; Validated |
143-695 |
8.55e-30 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 180988 [Multi-domain] Cd Length: 528 Bit Score: 124.38 E-value: 8.55e-30
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 143 NGDKIAMYWegnepsQDGKLTYSELLEKVCQLANYLKSLGVGKGDAVIIYLPMLLELPIAMLACARIGAVhsVVFAGF-- 220
Cdd:PRK07470 20 FPDRIALVW------GDRSWTWREIDARVDALAAALAARGVRKGDRILVHSRNCNQMFESMFAAFRLGAV--WVPTNFrq 91
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 221 SADSLAQRIVDCKPKLVItCNAVKRGvkpiflkdivDVALVESEknGVSVGLCLTYENQSAmkredtkwqpERDVwwQDV 300
Cdd:PRK07470 92 TPDEVAYLAEASGARAMI-CHADFPE----------HAAAVRAA--SPDLTHVVAIGGARA----------GLDY--EAL 146
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 301 VTKFP-TKCDVEWVDAEDPLFLLYTSGSTGKPK-GVLhtSGGYMVYTATTfkYAFDYKP--TDiywctADCgwitghSYV 376
Cdd:PRK07470 147 VARHLgARVANAAVDHDDPCWFFFTSGTTGRPKaAVL--THGQMAFVITN--HLADLMPgtTE-----QDA------SLV 211
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 377 TyGPLLNGA-------------TVLvfegTPNYP-DSGRCWDIVDKYNVTIFYTAPTLVRSLMRDgtEYVTRYSRKSLRV 442
Cdd:PRK07470 212 V-APLSHGAgihqlcqvargaaTVL----LPSERfDPAEVWALVERHRVTNLFTVPTILKMLVEH--PAVDRYDHSSLRY 284
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 443 LGSVGEP------------INPSAWRWFynivGDSRCpisdtwwqteTGGfmITPLPGAW------PQ-KPGSATFPFFG 503
Cdd:PRK07470 285 VIYAGAPmyradqkralakLGKVLVQYF----GLGEV----------TGN--ITVLPPALhdaedgPDaRIGTCGFERTG 348
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 504 VQPVIVDEKGQEIEGECSGYLCIKKswPGAFRTLYGDHERYEttyfKPFAGYYF-TGDGCSRDKDGYHWLTGRVDDVINV 582
Cdd:PRK07470 349 MEVQIQDDEGRELPPGETGEICVIG--PAVFAGYYNNPEANA----KAFRDGWFrTGDLGHLDARGFLYITGRASDMYIS 422
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 583 SGHRIGTAEVESALVSHPQCAEAAVVGVEHEVKGQGIYAFVTLVDGVPYSEElrkSLIMTVRNQIGAFAAPDKIHWAPGL 662
Cdd:PRK07470 423 GGSNVYPREIEEKLLTHPAVSEVAVLGVPDPVWGEVGVAVCVARDGAPVDEA---ELLAWLDGKVARYKLPKRFFFWDAL 499
|
570 580 590
....*....|....*....|....*....|...
gi 242061796 663 PKTRSGKIMRRILRKiaarQLDELGDISTLAEP 695
Cdd:PRK07470 500 PKSGYGKITKKMVRE----ELEERGLLDLERAP 528
|
|
| PRK06087 |
PRK06087 |
medium-chain fatty-acid--CoA ligase; |
163-683 |
6.53e-29 |
|
medium-chain fatty-acid--CoA ligase;
Pssm-ID: 180393 [Multi-domain] Cd Length: 547 Bit Score: 121.78 E-value: 6.53e-29
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 163 TYSELLEKVCQLANYLKSLGVGKGDAVIIYLPMLLELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPKLVITcna 242
Cdd:PRK06087 51 TYSALDHAASRLANWLLAKGIEPGDRVAFQLPGWCEFTIIYLACLKVGAVSVPLLPSWREAELVWVLNKCQAKMFFA--- 127
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 243 vkrgvkPIFLKDIVDVALVESEKNGV-SVGLCLTYENQSAMKREDTKWQperdvwwqdVVTKFPTKCDVEWVDAEDPLFL 321
Cdd:PRK06087 128 ------PTLFKQTRPVDLILPLQNQLpQLQQIVGVDKLAPATSSLSLSQ---------IIADYEPLTTAITTHGDELAAV 192
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 322 LYTSGSTGKPKGVLHTSGGyMVYTATTFKYAFDYKPTDIYWCTADCGWITG-HSYVTYGPLLNGATVLVFEGTPNypdsg 400
Cdd:PRK06087 193 LFTSGTEGLPKGVMLTHNN-ILASERAYCARLNLTWQDVFMMPAPLGHATGfLHGVTAPFLIGARSVLLDIFTPD----- 266
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 401 RCWDIVDKYNVTIFYTAPTLVRSLMRDGTEYVTRYSrkSLRVLGSVGEPInPS-----AWRwfYNIVgdsrcpISDTWWQ 475
Cdd:PRK06087 267 ACLALLEQQRCTCMLGATPFIYDLLNLLEKQPADLS--ALRFFLCGGTTI-PKkvareCQQ--RGIK------LLSVYGS 335
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 476 TETGGFMITPLPGAWPQKPGSATFPFFGVQPVIVDEKGQEIEGECSGYLCIKKswPGAFRTLYGDHERyeTTYFKPFAGY 555
Cdd:PRK06087 336 TESSPHAVVNLDDPLSRFMHTDGYAAAGVEIKVVDEARKTLPPGCEGEEASRG--PNVFMGYLDEPEL--TARALDEEGW 411
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 556 YFTGDGCSRDKDGYHWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAAVVGVEHEVKGQGIYAFVTLVDGVpYSEEL 635
Cdd:PRK06087 412 YYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVEDILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPH-HSLTL 490
|
490 500 510 520
....*....|....*....|....*....|....*....|....*...
gi 242061796 636 RKSLIMTVRNQIGAFAAPDKIHWAPGLPKTRSGKIMRRILRKIAARQL 683
Cdd:PRK06087 491 EEVVAFFSRKRVAKYKYPEHIVVIDKLPRTASGKIQKFLLRKDIMRRL 538
|
|
| AMP-binding_C |
pfam13193 |
AMP-binding enzyme C-terminal domain; This is a small domain that is found C terminal to ... |
591-669 |
3.26e-28 |
|
AMP-binding enzyme C-terminal domain; This is a small domain that is found C terminal to pfam00501. It has a central beta sheet core that is flanked by alpha helices.
Pssm-ID: 463804 [Multi-domain] Cd Length: 76 Bit Score: 108.02 E-value: 3.26e-28
10 20 30 40 50 60 70
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 242061796 591 EVESALVSHPQCAEAAVVGVEHEVKGQGIYAFVTLVDGVpysEELRKSLIMTVRNQIGAFAAPDKIHWAPGLPKTRSGK 669
Cdd:pfam13193 1 EVESALVSHPAVAEAAVVGVPDELKGEAPVAFVVLKPGV---ELLEEELVAHVREELGPYAVPKEVVFVDELPKTRSGK 76
|
|
| PRK06178 |
PRK06178 |
acyl-CoA synthetase; Validated |
145-681 |
5.95e-28 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 235724 [Multi-domain] Cd Length: 567 Bit Score: 118.99 E-value: 5.95e-28
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 145 DKIAMYWEGNEpsqdgkLTYSELLEKVCQLANYLKSLGVGKGDAVIIYLPMLLELPIAMLACARIGAVHSVVFAGFSADS 224
Cdd:PRK06178 48 QRPAIIFYGHV------ITYAELDELSDRFAALLRQRGVGAGDRVAVFLPNCPQFHIVFFGILKLGAVHVPVSPLFREHE 121
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 225 LAQRIVDCKPKLVITCNAVkrgvkpiflkdivdVALVESEKNGVSVGLCLTYENQSAMKREDTKWQPE-------RDVWW 297
Cdd:PRK06178 122 LSYELNDAGAEVLLALDQL--------------APVVEQVRAETSLRHVIVTSLADVLPAEPTLPLPDslraprlAAAGA 187
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 298 QDVVTKF---PTKCDVEWVDAEDPLFLLYTSGSTGKPKGVLHTSGgYMVYTATTFKYAFDYKPTD--------IYWctad 366
Cdd:PRK06178 188 IDLLPALracTAPVPLPPPALDALAALNYTGGTTGMPKGCEHTQR-DMVYTAAAAYAVAVVGGEDsvflsflpEFW---- 262
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 367 cgwITGHSYVTYGPLLNGATVLVFegtpNYPDSGRCWDIVDKYNVTIfytAPTLVRS---LMRdgTEYVTRYSRKSLRVL 443
Cdd:PRK06178 263 ---IAGENFGLLFPLFSGATLVLL----ARWDAVAFMAAVERYRVTR---TVMLVDNaveLMD--HPRFAEYDLSSLRQV 330
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 444 GSVG--EPINPsawrwfynivgDSRcpisdTWWQTETGGFMITplpGAW--PQKPGSATF--------------PFFGVQ 505
Cdd:PRK06178 331 RVVSfvKKLNP-----------DYR-----QRWRALTGSVLAE---AAWgmTETHTCDTFtagfqdddfdllsqPVFVGL 391
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 506 PV------IVDEKGQEI-----EGEcsgyLCIKKswPGAFRTLYGdheRYETTYFKPFAGYYFTGDGCSRDKDGY-HWLt 573
Cdd:PRK06178 392 PVpgtefkICDFETGELlplgaEGE----IVVRT--PSLLKGYWN---KPEATAEALRDGWLHTGDIGKIDEQGFlHYL- 461
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 574 GRVDDVINVSGHRIGTAEVESALVSHPQCAEAAVVGVEHEVKGQGIYAFVTLVDGVPYSEElrkSLIMTVRNQIGAFAAP 653
Cdd:PRK06178 462 GRRKEMLKVNGMSVFPSEVEALLGQHPAVLGSAVVGRPDPDKGQVPVAFVQLKPGADLTAA---ALQAWCRENMAVYKVP 538
|
570 580
....*....|....*....|....*...
gi 242061796 654 DkIHWAPGLPKTRSGKIMRRILRKIAAR 681
Cdd:PRK06178 539 E-IRIVDALPMTATGKVRKQDLQALAEE 565
|
|
| PRK06710 |
PRK06710 |
long-chain-fatty-acid--CoA ligase; Validated |
162-675 |
1.06e-27 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 180666 [Multi-domain] Cd Length: 563 Bit Score: 118.21 E-value: 1.06e-27
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 162 LTYSELLEKVCQLANYLKSLGVGKGDAVIIYLPMLLELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPKLVITCN 241
Cdd:PRK06710 50 ITFSVFHDKVKRFANYLQKLGVEKGDRVAIMLPNCPQAVIGYYGTLLAGGIVVQTNPLYTERELEYQLHDSGAKVILCLD 129
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 242 AVKRGVKPIFLKDIVDVALVESEKNGVSVGLCLTYenqSAMKREDTKW-----QPERDVWWQDVVTKFPTKCDVEwVDAE 316
Cdd:PRK06710 130 LVFPRVTNVQSATKIEHVIVTRIADFLPFPKNLLY---PFVQKKQSNLvvkvsESETIHLWNSVEKEVNTGVEVP-CDPE 205
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 317 DPLFLL-YTSGSTGKPKGVLHTSGGYMVYTATTFKYAFDYKP-TDIYWCTADCGWITGHSYVTYGPLLNGATVLVFegtP 394
Cdd:PRK06710 206 NDLALLqYTGGTTGFPKGVMLTHKNLVSNTLMGVQWLYNCKEgEEVVLGVLPFFHVYGMTAVMNLSIMQGYKMVLI---P 282
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 395 NYpDSGRCWDIVDKYNVTIFYTAPTLVRSLMRdgTEYVTRYSRKSLRVLGSVGEPINPSAWRWFYNIVGDSrcpISDTWW 474
Cdd:PRK06710 283 KF-DMKMVFEAIKKHKVTLFPGAPTIYIALLN--SPLLKEYDISSIRACISGSAPLPVEVQEKFETVTGGK---LVEGYG 356
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 475 QTETGGfmITPLPGAWPQK-PGSATFPFFGVQPVIVD-EKGQEIEGECSGYLCIKKSwpgafRTLYGDHERYETTYFKPF 552
Cdd:PRK06710 357 LTESSP--VTHSNFLWEKRvPGSIGVPWPDTEAMIMSlETGEALPPGEIGEIVVKGP-----QIMKGYWNKPEETAAVLQ 429
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 553 AGYYFTGDGCSRDKDGYHWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAAVVGVEHEVKGQGIYAFVTLVDGVPYS 632
Cdd:PRK06710 430 DGWLHTGDVGYMDEDGFFYVKDRKKDMIVASGFNVYPREVEEVLYEHEKVQEVVTIGVPDPYRGETVKAFVVLKEGTECS 509
|
490 500 510 520
....*....|....*....|....*....|....*....|...
gi 242061796 633 EElrkSLIMTVRNQIGAFAAPDKIHWAPGLPKTRSGKIMRRIL 675
Cdd:PRK06710 510 EE---ELNQFARKYLAAYKVPKVYEFRDELPKTTVGKILRRVL 549
|
|
| PRK12316 |
PRK12316 |
peptide synthase; Provisional |
159-683 |
1.68e-27 |
|
peptide synthase; Provisional
Pssm-ID: 237054 [Multi-domain] Cd Length: 5163 Bit Score: 120.06 E-value: 1.68e-27
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 159 DGKLTYSELLEKVCQLANYLKSLGVGKGDAVIIYLPMLLELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPKLVI 238
Cdd:PRK12316 2026 DQHLSYAELDSRANRLAHRLRARGVGPEVRVAIAAERSFELVVALLAVLKAGGAYVPLDPNYPAERLAYMLEDSGAALLL 2105
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 239 TcnavKRGVkpiflkdivdvalveSEKNGVSVGL-CLTYEnqsamkredtkwqpeRDVWWQDvvtkFPTKCDVEWVDAED 317
Cdd:PRK12316 2106 T----QRHL---------------LERLPLPAGVaRLPLD---------------RDAEWAD----YPDTAPAVQLAGEN 2147
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 318 PLFLLYTSGSTGKPKGVLHTSGGYMVYTATTFKYafdYKPTDiywctADC--------------GWITghsyvtygPLLN 383
Cdd:PRK12316 2148 LAYVIYTSGSTGLPKGVAVSHGALVAHCQAAGER---YELSP-----ADCelqfmsfsfdgaheQWFH--------PLLN 2211
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 384 GATVLVFEGTpnYPDSGRCWDIVDKYNVTIFYTAPTLVRSLM----RDGteyvtrySRKSLRVLGSVGEPINPSAWRWFY 459
Cdd:PRK12316 2212 GARVLIRDDE--LWDPEQLYDEMERHGVTILDFPPVYLQQLAehaeRDG-------RPPAVRVYCFGGEAVPAASLRLAW 2282
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 460 NIVGDSRcpISDTWWQTETggfMITPLpgAW---PQKPGSATFPFFGvQPV------IVDEKGQEIEGECSGYLCIkksw 530
Cdd:PRK12316 2283 EALRPVY--LFNGYGPTEA---VVTPL--LWkcrPQDPCGAAYVPIG-RALgnrrayILDADLNLLAPGMAGELYL---- 2350
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 531 pGAFRTLYGDHERYETTYFK----PFAG----YYFTGDGCSRDKDGYHWLTGRVDDVINVSGHRIGTAEVESALVSHPQC 602
Cdd:PRK12316 2351 -GGEGLARGYLNRPGLTAERfvpdPFSAsgerLYRTGDLARYRADGVVEYLGRIDHQVKIRGFRIELGEIEARLQAHPAV 2429
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 603 AEAAVVGVEhEVKGQGIYAFVTLVDGvpySEELRKSLIMTVRNQIGAFAAPDKIHWAPGLPKTRSGKIMRRILRKIAARQ 682
Cdd:PRK12316 2430 REAVVVAQD-GASGKQLVAYVVPDDA---AEDLLAELRAWLAARLPAYMVPAHWVVLERLPLNPNGKLDRKALPKPDVSQ 2505
|
.
gi 242061796 683 L 683
Cdd:PRK12316 2506 L 2506
|
|
| PRK03640 |
PRK03640 |
o-succinylbenzoate--CoA ligase; |
145-678 |
1.77e-27 |
|
o-succinylbenzoate--CoA ligase;
Pssm-ID: 235146 [Multi-domain] Cd Length: 483 Bit Score: 116.60 E-value: 1.77e-27
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 145 DKIAMYWEgnepsqDGKLTYSELLEKVCQLANYLKSLGVGKGDAVIIYLPMLLELPIAMLACARIGAVhsVVFAG--FSA 222
Cdd:PRK03640 17 DRTAIEFE------EKKVTFMELHEAVVSVAGKLAALGVKKGDRVALLMKNGMEMILVIHALQQLGAV--AVLLNtrLSR 88
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 223 DSLAQRIVDCKPKLVITcnavkrgvKPIFLKDIVdvalvesekngvsVGLCLTYENQSAMKREDTKWQPERDVwwQDVVT 302
Cdd:PRK03640 89 EELLWQLDDAEVKCLIT--------DDDFEAKLI-------------PGISVKFAELMNGPKEEAEIQEEFDL--DEVAT 145
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 303 kfptkcdvewvdaedplfLLYTSGSTGKPKGVLHTSGGYMvYTATTfkYAFDYKPTDiywctADCgW--------ITGHS 374
Cdd:PRK03640 146 ------------------IMYTSGTTGKPKGVIQTYGNHW-WSAVG--SALNLGLTE-----DDC-WlaavpifhISGLS 198
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 375 YVTYGpLLNGATVLVFEGTpnypDSGRCWDIVDKYNVTIFYTAPTLVRSLMRDGTEyvTRYSrKSLR--VLGsvGEPINP 452
Cdd:PRK03640 199 ILMRS-VIYGMRVVLVEKF----DAEKINKLLQTGGVTIISVVSTMLQRLLERLGE--GTYP-SSFRcmLLG--GGPAPK 268
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 453 S----AWRWfyNIvgdsrcPISDTWWQTETGGFMITPLPGAWPQKPGSATFPFFGVQPVIVDEkGQEI----EGE----- 519
Cdd:PRK03640 269 PlleqCKEK--GI------PVYQSYGMTETASQIVTLSPEDALTKLGSAGKPLFPCELKIEKD-GVVVppfeEGEivvkg 339
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 520 ---CSGYLCIKKSWPGAFRTlygdheryettyfkpfaGYYFTGDGCSRDKDGYHWLTGRVDDVINVSGHRIGTAEVESAL 596
Cdd:PRK03640 340 pnvTKGYLNREDATRETFQD-----------------GWFKTGDIGYLDEEGFLYVLDRRSDLIISGGENIYPAEIEEVL 402
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 597 VSHPQCAEAAVVGVEHEVKGQGIYAFVtlVDGVPYSEElrkSLIMTVRNQIGAFAAPDKIHWAPGLPKTRSGKIMRRILR 676
Cdd:PRK03640 403 LSHPGVAEAGVVGVPDDKWGQVPVAFV--VKSGEVTEE---ELRHFCEEKLAKYKVPKRFYFVEELPRNASGKLLRHELK 477
|
..
gi 242061796 677 KI 678
Cdd:PRK03640 478 QL 479
|
|
| PRK06839 |
PRK06839 |
o-succinylbenzoate--CoA ligase; |
145-678 |
2.14e-27 |
|
o-succinylbenzoate--CoA ligase;
Pssm-ID: 168698 [Multi-domain] Cd Length: 496 Bit Score: 116.50 E-value: 2.14e-27
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 145 DKIAMYweGNEPSqdgkLTYSELLEKVCQLANYLK-SLGVGKGDAVIIYLPMLLELPIAMLACARIGAVhsvvfagfsad 223
Cdd:PRK06839 17 DRIAII--TEEEE----MTYKQLHEYVSKVAAYLIyELNVKKGERIAILSQNSLEYIVLLFAIAKVECI----------- 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 224 slaqrivdckpklvitcnAVkrgvkPIFLKDIVDVALVESEKNGVSVgLCLTYENQSAMKREDTKWQPERDVWwqdvVTK 303
Cdd:PRK06839 80 ------------------AV-----PLNIRLTENELIFQLKDSGTTV-LFVEKTFQNMALSMQKVSYVQRVIS----ITS 131
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 304 FPTKCDVEWVDAED-----PLFLLYTSGSTGKPKGVLHTSGGyMVYTATTFKYAFDYKPTDIYWCTADCGWITGHSYVTY 378
Cdd:PRK06839 132 LKEIEDRKIDNFVEknesaSFIICYTSGTTGKPKGAVLTQEN-MFWNALNNTFAIDLTMHDRSIVLLPLFHIGGIGLFAF 210
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 379 GPLLNGATVLVfegtPNYPDSGRCWDIVDKYNVTIFYTAPTLVRSLMRdgteyvtrysrkslrvlGSVGEPINPSAWRWF 458
Cdd:PRK06839 211 PTLFAGGVIIV----PRKFEPTKALSMIEKHKVTVVMGVPTIHQALIN-----------------CSKFETTNLQSVRWF 269
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 459 YNivGDSRCPI-------------SDTWWQTETGG--FMITPLPGAwpQKPGSATFPFFGVQPVIVDEKGQEIEGECSGY 523
Cdd:PRK06839 270 YN--GGAPCPEelmrefidrgflfGQGFGMTETSPtvFMLSEEDAR--RKVGSIGKPVLFCDYELIDENKNKVEVGEVGE 345
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 524 LCIKKswPGAFRTLYGDHERYETTYFKpfaGYYFTGDGCSRDKDGYHWLTGRVDDVINVSGHRIGTAEVESALVSHPQCA 603
Cdd:PRK06839 346 LLIRG--PNVMKEYWNRPDATEETIQD---GWLCTGDLARVDEDGFVYIVGRKKEMIISGGENIYPLEVEQVINKLSDVY 420
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 242061796 604 EAAVVGVEHEVKGQGIYAFVTLVDGVPYSEElrkSLIMTVRNQIGAFAAPDKIHWAPGLPKTRSGKIMRRILRKI 678
Cdd:PRK06839 421 EVAVVGRQHVKWGEIPIAFIVKKSSSVLIEK---DVIEHCRLFLAKYKIPKEIVFLKELPKNATGKIQKAQLVNQ 492
|
|
| PRK07638 |
PRK07638 |
acyl-CoA synthetase; Validated |
145-683 |
2.31e-27 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236071 [Multi-domain] Cd Length: 487 Bit Score: 116.42 E-value: 2.31e-27
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 145 DKIAMYWEgnepsqDGKLTYSELLEKVCQLANYLKSLGvGKGDAVIIYLPMLLELPIAMLACARIGAVHSVVFAGFSADS 224
Cdd:PRK07638 16 NKIAIKEN------DRVLTYKDWFESVCKVANWLNEKE-SKNKTIAILLENRIEFLQLFAGAAMAGWTCVPLDIKWKQDE 88
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 225 LAQRIVDCKPKLVITcnavkrgvKPIFLKDIVDV--ALVESEKngvsvglcltyenqsamkredtkwqperdvWWQDVVT 302
Cdd:PRK07638 89 LKERLAISNADMIVT--------ERYKLNDLPDEegRVIEIDE------------------------------WKRMIEK 130
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 303 KFPT---KCDVEwvdaEDPLFLLYTSGSTGKPKGVLHTSGGYMvytattfkYAFDYKPTDIYWCTADCGWITG---HSYV 376
Cdd:PRK07638 131 YLPTyapIENVQ----NAPFYMGFTSGSTGKPKAFLRAQQSWL--------HSFDCNVHDFHMKREDSVLIAGtlvHSLF 198
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 377 TYGP---LLNGATVLVFEG-TPNypdsgRCWDIVDKYNVTIFYTAPTLVRSLMRdgteyVTRYSRKSLRVLGSVGE---- 448
Cdd:PRK07638 199 LYGAistLYVGQTVHLMRKfIPN-----QVLDKLETENISVMYTVPTMLESLYK-----ENRVIENKMKIISSGAKweae 268
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 449 ------PINPSAWRW-FYnivGDSRCpisdtwwqtetgGFMITPLPGAWPQKPGSATFPFFGVQPVIVDEKGQEIEGECS 521
Cdd:PRK07638 269 akekikNIFPYAKLYeFY---GASEL------------SFVTALVDEESERRPNSVGRPFHNVQVRICNEAGEEVQKGEI 333
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 522 GYLCIKKswPGAFRTLYGDHERYETTyfkPFAGYYFTGDGCSRDKDGYHWLTGRVDDVINVSGHRIGTAEVESALVSHPQ 601
Cdd:PRK07638 334 GTVYVKS--PQFFMGYIIGGVLAREL---NADGWMTVRDVGYEDEEGFIYIVGREKNMILFGGINIFPEEIESVLHEHPA 408
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 602 CAEAAVVGVEHEVKGQgiyAFVTLVDGVPYSEELRKSLimtvRNQIGAFAAPDKIHWAPGLPKTRSGKIMRRILRKIAAR 681
Cdd:PRK07638 409 VDEIVVIGVPDSYWGE---KPVAIIKGSATKQQLKSFC----LQRLSSFKIPKEWHFVDEIPYTNSGKIARMEAKSWIEN 481
|
..
gi 242061796 682 QL 683
Cdd:PRK07638 482 QE 483
|
|
| A_NRPS_PpsD_like |
cd17650 |
similar to adenylation domain of plipastatin synthase (PpsD); This family of the adenylation ... |
161-675 |
3.83e-27 |
|
similar to adenylation domain of plipastatin synthase (PpsD); This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes bacitracin synthetase 1 (BacA) in Bacillus licheniformis, tyrocidine synthetase in Brevibacillus brevis, plipastatin synthase (PpsD, an important antifungal protein) in Bacillus subtilis and mannopeptimycin peptide synthetase (MppB) in Streptomyces hygroscopicus. Plipastatin has strong fungitoxic activity and is involved in inhibition of phospholipase A2 and biofilm formation. Bacitracin, a mixture of related cyclic peptides, is used as a polypeptide antibiotic while function of tyrocidine is thought to be regulation of sporulation. MppB is involved in biosynthetic pathway of mannopeptimycin, a novel class of mannosylated lipoglycopeptides. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341305 [Multi-domain] Cd Length: 447 Bit Score: 115.26 E-value: 3.83e-27
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 161 KLTYSELLEKVCQLANYLKSLGVGKGDAVIIYLPMLLELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPKLVITc 240
Cdd:cd17650 12 QLTYRELNERANQLARTLRGLGVAPGSVVGVCADRSLDAIVGLLAVLKAGGAYVPIDPDYPAERLQYMLEDSGAKLLLT- 90
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 241 navkrgvkpiflkdivdvalvesekngvsvglcltyenqsamkredtkwQPErdvwwqdvvtkfptkcdvewvdaeDPLF 320
Cdd:cd17650 91 -------------------------------------------------QPE------------------------DLAY 97
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 321 LLYTSGSTGKPKGVL--HTSGGYMVYTATTfKYAFDYKPTDIYWctadcgwITGHSY-VTYG----PLLNGATVLVfegT 393
Cdd:cd17650 98 VIYTSGTTGKPKGVMveHRNVAHAAHAWRR-EYELDSFPVRLLQ-------MASFSFdVFAGdfarSLLNGGTLVI---C 166
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 394 PN--YPDSGRCWDIVDKYNVTIFYTAPTLVRSLMrdgtEYVTRYSRK--SLRVLgSVGEPINPSAW-RWFY--------- 459
Cdd:cd17650 167 PDevKLDPAALYDLILKSRITLMESTPALIRPVM----AYVYRNGLDlsAMRLL-IVGSDGCKAQDfKTLAarfgqgmri 241
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 460 -NIVGDSRCPISDTWWQTETGgfmitPLPGAWPQKPGSatfPFFGVQPVIVDEKGQEIEGECSGYLCIKKSwpGAFRTLY 538
Cdd:cd17650 242 iNSYGVTEATIDSTYYEEGRD-----PLGDSANVPIGR---PLPNTAMYVLDERLQPQPVGVAGELYIGGA--GVARGYL 311
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 539 GD----HERYETTYFKPFAGYYFTGDGCSRDKDGYHWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAAVVgVEHEV 614
Cdd:cd17650 312 NRpeltAERFVENPFAPGERMYRTGDLARWRADGNVELLGRVDHQVKIRGFRIELGEIESQLARHPAIDEAVVA-VREDK 390
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|.
gi 242061796 615 KGQGIYAFVTLVDGVPYSEELRKSLImtvrNQIGAFAAPDKIHWAPGLPKTRSGKIMRRIL 675
Cdd:cd17650 391 GGEARLCAYVVAAATLNTAELRAFLA----KELPSYMIPSYYVQLDALPLTPNGKVDRRAL 447
|
|
| PRK07788 |
PRK07788 |
acyl-CoA synthetase; Validated |
159-678 |
5.46e-27 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236097 [Multi-domain] Cd Length: 549 Bit Score: 115.79 E-value: 5.46e-27
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 159 DGKLTYSELLEKVCQLANYLKSLGVGKGDAVIIYLPMLLELPIAMLACARIGAVHSVVFAGFSADslaqRIVDCkpklvi 238
Cdd:PRK07788 72 RGTLTYAELDEQSNALARGLLALGVRAGDGVAVLARNHRGFVLALYAAGKVGARIILLNTGFSGP----QLAEV------ 141
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 239 tcnAVKRGVKPIFLKDIVD--VALVESEkngvsVGLCLTYenqsAMKREDTKWQPERDVWWQDVV-----TKFPTkcdve 311
Cdd:PRK07788 142 ---AAREGVKALVYDDEFTdlLSALPPD-----LGRLRAW----GGNPDDDEPSGSTDETLDDLIagsstAPLPK----- 204
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 312 wvdAEDP-LFLLYTSGSTGKPKGVLHT------------------SGGYMVYTATTFKYafdykptdiyWCTADCGWITG 372
Cdd:PRK07788 205 ---PPKPgGIVILTSGTTGTPKGAPRPepsplaplagllsrvpfrAGETTLLPAPMFHA----------TGWAHLTLAMA 271
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 373 HsyvtygpllnGATVLV---FegtpnypDSGRCWDIVDKYNVTIFYTAPTLVRSLMRDGTEYVTRYSRKSLRVLGSVGEP 449
Cdd:PRK07788 272 L----------GSTVVLrrrF-------DPEATLEDIAKHKATALVVVPVMLSRILDLGPEVLAKYDTSSLKIIFVSGSA 334
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 450 INPSAWRWFYNIVGDSRCpisDTWWQTETGgFMITPLPGAWPQKPGSATFPFFGVQPVIVDEKGQEIEGECSGYLCIKKS 529
Cdd:PRK07788 335 LSPELATRALEAFGPVLY---NLYGSTEVA-FATIATPEDLAEAPGTVGRPPKGVTVKILDENGNEVPRGVVGRIFVGNG 410
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 530 WPgaFrtlygdhERYETTYFKPFA-GYYFTGDGCSRDKDGYHWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAAVV 608
Cdd:PRK07788 411 FP--F-------EGYTDGRDKQIIdGLLSSGDVGYFDEDGLLFVDGRDDDMIVSGGENVFPAEVEDLLAGHPDVVEAAVI 481
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 609 GVEHEVKGQGIYAFVTLVDGVPYSEELRKSLimtVRNQIGAFAAPDKIHWAPGLPKTRSGKIMRRILRKI 678
Cdd:PRK07788 482 GVDDEEFGQRLRAFVVKAPGAALDEDAIKDY---VRDNLARYKVPRDVVFLDELPRNPTGKVLKRELREM 548
|
|
| A_NRPS_CmdD_like |
cd17652 |
similar to adenylation domain of chondramide synthase cmdD; This family of the adenylation (A) ... |
159-675 |
1.52e-26 |
|
similar to adenylation domain of chondramide synthase cmdD; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes phosphinothricin tripeptide (PTT, phosphinothricylalanylalanine) synthetase, where PTT is a natural-product antibiotic and potent herbicide that is produced by Streptomyces hygroscopicus. This adenylation domain has been confirmed to directly activate beta-tyrosine, and fluorinated chondramides are produced through precursor-directed biosynthesis. Also included in this family is chondramide synthase D (also known as ATP-dependent phenylalanine adenylase or phenylalanine activase or tyrosine activase). Chondramides A-D are depsipeptide antitumor and antifungal antibiotics produced by C. crocatus, are a class of mixed peptide/polyketide depsipeptides comprised of three amino acids (alanine, N-methyltryptophan, plus the unusual amino acid beta-tyrosine or alpha-methoxy-beta-tyrosine) and a polyketide chain ([E]-7-hydroxy-2,4,6-trimethyloct-4-enoic acid).
Pssm-ID: 341307 [Multi-domain] Cd Length: 436 Bit Score: 113.12 E-value: 1.52e-26
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 159 DGKLTYSELLEKVCQLANYLKSLGVGKGDAVIIYLPMLLELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPKLVI 238
Cdd:cd17652 10 DETLTYAELNARANRLARLLAARGVGPERLVALALPRSAELVVAILAVLKAGAAYLPLDPAYPAERIAYMLADARPALLL 89
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 239 TcnavkrgvkpiflkdivdvalvesekngvsvglcltyenqsamkredtkwqperdvwwqdvvtkfptkcdvewvDAEDP 318
Cdd:cd17652 90 T--------------------------------------------------------------------------TPDNL 95
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 319 LFLLYTSGSTGKPKGVL--HTSGGYMVYTATTfkyAFDYKPTDiywCTADCGWITGHSYVT--YGPLLNGATVLVfegtp 394
Cdd:cd17652 96 AYVIYTSGSTGRPKGVVvtHRGLANLAAAQIA---AFDVGPGS---RVLQFASPSFDASVWelLMALLAGATLVL----- 164
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 395 nyPDSGRCW------DIVDKYNVTIFYTAPTLVRSLMRDGTEyvtrysrkSLRVLGSVGEPINPS-AWRW-----FYNIV 462
Cdd:cd17652 165 --APAEELLpgeplaDLLREHRITHVTLPPAALAALPPDDLP--------DLRTLVVAGEACPAElVDRWapgrrMINAY 234
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 463 GDSRCPISDTWWQTETGGFMItPLPGAWPqkpgsatfpffGVQPVIVDEKGQEIEGECSGYLCIkkSWPGAFRtlyGDHE 542
Cdd:cd17652 235 GPTETTVCATMAGPLPGGGVP-PIGRPVP-----------GTRVYVLDARLRPVPPGVPGELYI--AGAGLAR---GYLN 297
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 543 RYETTYFK----PFAG----YYFTGDGCSRDKDGYHWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAAVVGVEHEV 614
Cdd:cd17652 298 RPGLTAERfvadPFGApgsrMYRTGDLARWRADGQLEFLGRADDQVKIRGFRIELGEVEAALTEHPGVAEAVVVVRDDRP 377
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|..
gi 242061796 615 KGQGIYAFVTLVDGV-PYSEELRKSLimtvRNQIGAFAAPDKIHWAPGLPKTRSGKIMRRIL 675
Cdd:cd17652 378 GDKRLVAYVVPAPGAaPTAAELRAHL----AERLPGYMVPAAFVVLDALPLTPNGKLDRRAL 435
|
|
| PRK13390 |
PRK13390 |
acyl-CoA synthetase; Provisional |
315-676 |
2.38e-26 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 139538 [Multi-domain] Cd Length: 501 Bit Score: 113.57 E-value: 2.38e-26
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 315 AEDPL--FLLYTSGSTGKPKGV--------LHTSGGYMVYTATTFkyaFDYKPTDIYWCTADCGWITGHSYVTYGPLLNG 384
Cdd:PRK13390 145 TEQPCgaVMLYSSGTTGFPKGIqpdlpgrdVDAPGDPIVAIARAF---YDISESDIYYSSAPIYHAAPLRWCSMVHALGG 221
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 385 ATVLVfegtpNYPDSGRCWDIVDKYNVTIFYTAPTLVRSLMRDGTEYVTRYSRKSLRVLGSVGEP----INPSAWRWFYN 460
Cdd:PRK13390 222 TVVLA-----KRFDAQATLGHVERYRITVTQMVPTMFVRLLKLDADVRTRYDVSSLRAVIHAAAPcpvdVKHAMIDWLGP 296
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 461 IvgdsrcpISDTWWQTETGGFMITPLPgAWPQKPGSATFPFFGVQPvIVDEKGQEI-EGECSGYLCIKKSWPgaFRTLYG 539
Cdd:PRK13390 297 I-------VYEYYSSTEAHGMTFIDSP-DWLAHPGSVGRSVLGDLH-ICDDDGNELpAGRIGTVYFERDRLP--FRYLND 365
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 540 DHERYETTYfkPFAGYYFT-GDGCSRDKDGYHWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAAVVGVEHEVKGQG 618
Cdd:PRK13390 366 PEKTAAAQH--PAHPFWTTvGDLGSVDEDGYLYLADRKSFMIISGGVNIYPQETENALTMHPAVHDVAVIGVPDPEMGEQ 443
|
330 340 350 360 370
....*....|....*....|....*....|....*....|....*....|....*...
gi 242061796 619 IYAFVTLVDGVPYSEELRKSLIMTVRNQIGAFAAPDKIHWAPGLPKTRSGKIMRRILR 676
Cdd:PRK13390 444 VKAVIQLVEGIRGSDELARELIDYTRSRIAHYKAPRSVEFVDELPRTPTGKLVKGLLR 501
|
|
| PLN02246 |
PLN02246 |
4-coumarate--CoA ligase |
162-680 |
2.98e-26 |
|
4-coumarate--CoA ligase
Pssm-ID: 215137 [Multi-domain] Cd Length: 537 Bit Score: 113.54 E-value: 2.98e-26
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 162 LTYSELLEKVCQLANYLKSLGVGKGDAVIIYLPMLLELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPKLVITCN 241
Cdd:PLN02246 51 YTYADVELLSRRVAAGLHKLGIRQGDVVMLLLPNCPEFVLAFLGASRRGAVTTTANPFYTPAEIAKQAKASGAKLIITQS 130
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 242 AVKRGVKPIFLKDIVDVALVESEKNGvsvglCLTYenqSAMKREDTKWQPERDVWWQDVVTkfptkcdvewvdaedplfL 321
Cdd:PLN02246 131 CYVDKLKGLAEDDGVTVVTIDDPPEG-----CLHF---SELTQADENELPEVEISPDDVVA------------------L 184
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 322 LYTSGSTGKPKGVLHTSGGYMvytaTTFKYAFDYKPTDIYWCTAD---CGWITGHSYVTYGPLLN----GATVLVFegtP 394
Cdd:PLN02246 185 PYSSGTTGLPKGVMLTHKGLV----TSVAQQVDGENPNLYFHSDDvilCVLPMFHIYSLNSVLLCglrvGAAILIM---P 257
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 395 NYpDSGRCWDIVDKYNVTIFYTAPTLVRSLMRdgTEYVTRYSRKSLRVLGSVGEPINPS---AWRwfynivgdSRCP--- 468
Cdd:PLN02246 258 KF-EIGALLELIQRHKVTIAPFVPPIVLAIAK--SPVVEKYDLSSIRMVLSGAAPLGKEledAFR--------AKLPnav 326
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 469 ISDTWWQTETG-------GFMITPLpgawPQKPGSATFPFFGVQPVIVD-EKGQEIEGECSGYLCIKKswPGAFRTLYGD 540
Cdd:PLN02246 327 LGQGYGMTEAGpvlamclAFAKEPF----PVKSGSCGTVVRNAELKIVDpETGASLPRNQPGEICIRG--PQIMKGYLND 400
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 541 HERYETTYFKpfAGYYFTGDGCSRDKDGYHWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAAVVGVEHEVKGQGIY 620
Cdd:PLN02246 401 PEATANTIDK--DGWLHTGDIGYIDDDDELFIVDRLKELIKYKGFQVAPAELEALLISHPSIADAAVVPMKDEVAGEVPV 478
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|.
gi 242061796 621 AFVTLVDGVPYSEELRKSLImtvRNQIGAFAAPDKIHWAPGLPKTRSGKIMRRILR-KIAA 680
Cdd:PLN02246 479 AFVVRSNGSEITEDEIKQFV---AKQVVFYKRIHKVFFVDSIPKAPSGKILRKDLRaKLAA 536
|
|
| BACL_like |
cd05929 |
Bacterial Bile acid CoA ligases and similar proteins; Bile acid-Coenzyme A ligase catalyzes ... |
321-676 |
3.92e-26 |
|
Bacterial Bile acid CoA ligases and similar proteins; Bile acid-Coenzyme A ligase catalyzes the formation of bile acid-CoA conjugates in a two-step reaction: the formation of a bile acid-AMP molecule as an intermediate, followed by the formation of a bile acid-CoA. This ligase requires a bile acid with a free carboxyl group, ATP, Mg2+, and CoA for synthesis of the final bile acid-CoA conjugate. The bile acid-CoA ligation is believed to be the initial step in the bile acid 7alpha-dehydroxylation pathway in the intestinal bacterium Eubacterium sp.
Pssm-ID: 341252 [Multi-domain] Cd Length: 473 Bit Score: 112.47 E-value: 3.92e-26
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 321 LLYTSGSTGKPKGVLHTSGGYMVYTATTFKYAFDykptdiywctadCGWITGHSYVTYGPLLNGA------TVLVFEGT- 393
Cdd:cd05929 130 MLYSGGTTGRPKGIKRGLPGGPPDNDTLMAAALG------------FGPGADSVYLSPAPLYHAApfrwsmTALFMGGTl 197
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 394 ---PNYpDSGRCWDIVDKYNVTIFYTAPTLVRSLMRDGTEYVTRYSRKSLRVLGSVGEPINPsawrWFYNIVGDSRCPIS 470
Cdd:cd05929 198 vlmEKF-DPEEFLRLIERYRVTFAQFVPTMFVRLLKLPEAVRNAYDLSSLKRVIHAAAPCPP----WVKEQWIDWGGPII 272
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 471 DTWWQ-TETGGFmiTPLPGA-WPQKPGSATFPFFGVQPvIVDEKGQEI----EGEcsgylcIKKSWPGAFrtLYgdHERY 544
Cdd:cd05929 273 WEYYGgTEGQGL--TIINGEeWLTHPGSVGRAVLGKVH-ILDEDGNEVppgeIGE------VYFANGPGF--EY--TNDP 339
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 545 ETTYFKPFAGYYFT-GDGCSRDKDGYHWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAAVVGVEHEVKGQGIYAFV 623
Cdd:cd05929 340 EKTAAARNEGGWSTlGDVGYLDEDGYLYLTDRRSDMIISGGVNIYPQEIENALIAHPKVLDAAVVGVPDEELGQRVHAVV 419
|
330 340 350 360 370
....*....|....*....|....*....|....*....|....*....|...
gi 242061796 624 TLVDGVPYSEELRKSLIMTVRNQIGAFAAPDKIHWAPGLPKTRSGKIMRRILR 676
Cdd:cd05929 420 QPAPGADAGTALAEELIAFLRDRLSRYKCPRSIEFVAELPRDDTGKLYRRLLR 472
|
|
| AFD_YhfT-like |
cd17633 |
fatty acid-CoA ligase VraA; This family of acyl-CoA ligases includes Bacillus subtilis YhfT, ... |
317-672 |
8.95e-26 |
|
fatty acid-CoA ligase VraA; This family of acyl-CoA ligases includes Bacillus subtilis YhfT, as well as long-chain fatty acid-CoA ligase VraA, all of which are as yet to be characterized. These proteins belong to the adenylate-forming enzymes which catalyze an ATP-dependent two-step reaction to first activate a carboxylate substrate as an adenylate and then transfer the carboxylate to the pantetheine group of either coenzyme A or an acyl-carrier protein. The active site of the domain is located at the interface of a large N-terminal subdomain and a smaller C-terminal subdomain
Pssm-ID: 341288 [Multi-domain] Cd Length: 320 Bit Score: 108.65 E-value: 8.95e-26
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 317 DPLFLLYTSGSTGKPKGVLHTSGGYMVytattfkyAFDYKPTDIYWCTADCGWITG---HSYVTYG---PLLNGATVLVF 390
Cdd:cd17633 1 NPFYIGFTSGTTGLPKAYYRSERSWIE--------SFVCNEDLFNISGEDAILAPGplsHSLFLYGaisALYLGGTFIGQ 72
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 391 EGTpnypDSGRCWDIVDKYNVTIFYTAPTLVRSLMRDGTeyvtrySRKSLRVLGSVGEPINPSAWRWFYNIVGDSRcpIS 470
Cdd:cd17633 73 RKF----NPKSWIRKINQYNATVIYLVPTMLQALARTLE------PESKIKSIFSSGQKLFESTKKKLKNIFPKAN--LI 140
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 471 DTWWQTETGgfMITPLPGAWPQKPGSATFPFFGVQPVIVDEKGQEIegecsGYLCIKKswPGAFrTLYGDHERYETTyfk 550
Cdd:cd17633 141 EFYGTSELS--FITYNFNQESRPPNSVGRPFPNVEIEIRNADGGEI-----GKIFVKS--EMVF-SGYVRGGFSNPD--- 207
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 551 pfaGYYFTGDGCSRDKDGYHWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAAVVGVEHEVKGQgIYAFVTLVDGVP 630
Cdd:cd17633 208 ---GWMSVGDIGYVDEEGYLYLVGRESDMIIIGGINIFPTEIESVLKAIPGIEEAIVVGIPDARFGE-IAVALYSGDKLT 283
|
330 340 350 360
....*....|....*....|....*....|....*....|..
gi 242061796 631 YSEELRKSLIMTVRNQIgafaaPDKIHWAPGLPKTRSGKIMR 672
Cdd:cd17633 284 YKQLKRFLKQKLSRYEI-----PKKIIFVDSLPYTSSGKIAR 320
|
|
| PRK05605 |
PRK05605 |
long-chain-fatty-acid--CoA ligase; Validated |
133-685 |
1.29e-25 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 235531 [Multi-domain] Cd Length: 573 Bit Score: 112.02 E-value: 1.29e-25
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 133 NAVDRNiesgnGDKIAMYWEGNEpsqdgkLTYSELLEKVCQLANYLKSLGVGKGDAVIIYLPMLLELPIAMLACARIGAV 212
Cdd:PRK05605 40 NAVARF-----GDRPALDFFGAT------TTYAELGKQVRRAAAGLRALGVRPGDRVAIVLPNCPQHIVAFYAVLRLGAV 108
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 213 hsVVFAG--FSADSLAQRIVDCKPKLVI----TCNAVKRGVKPIFLKDIVDVALVESEKNGVSVGLCLTYENQSAMKRED 286
Cdd:PRK05605 109 --VVEHNplYTAHELEHPFEDHGARVAIvwdkVAPTVERLRRTTPLETIVSVNMIAAMPLLQRLALRLPIPALRKARAAL 186
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 287 TKWQPERDVWWQDVVTKFP---TKCDVEWVDAEDPLFLLYTSGSTGKPKGVLHTSGGYMvytattfkyafdykptdiywC 363
Cdd:PRK05605 187 TGPAPGTVPWETLVDAAIGgdgSDVSHPRPTPDDVALILYTSGTTGKPKGAQLTHRNLF--------------------A 246
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 364 TADCG--WITG---------------HSY-----VTYGPLLNGATVLvfegTPNyPDSGRCWDIVDKYNVTIFYTAPTLV 421
Cdd:PRK05605 247 NAAQGkaWVPGlgdgpervlaalpmfHAYgltlcLTLAVSIGGELVL----LPA-PDIDLILDAMKKHPPTWLPGVPPLY 321
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 422 RSLMRDGTEYvtRYSRKSLRvlgsvgepinpsawrwfYNIVGDSRCPISDT-WWQTETGGFMI--------TPLPGAWP- 491
Cdd:PRK05605 322 EKIAEAAEER--GVDLSGVR-----------------NAFSGAMALPVSTVeLWEKLTGGLLVegygltetSPIIVGNPm 382
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 492 ---QKPGSATFPFFGVQPVIVDEK--------GQEieGEcsgyLCIKKswPGAFRtlyGDHERYETTYFKPFAGYYFTGD 560
Cdd:PRK05605 383 sddRRPGYVGVPFPDTEVRIVDPEdpdetmpdGEE--GE----LLVRG--PQVFK---GYWNRPEETAKSFLDGWFRTGD 451
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 561 GCSRDKDGYHWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAAVVGVEHEVKGQGIYAFVTLVDGVPYSEElrkSLI 640
Cdd:PRK05605 452 VVVMEEDGFIRIVDRIKELIITGGFNVYPAEVEEVLREHPGVEDAAVVGLPREDGSEEVVAAVVLEPGAALDPE---GLR 528
|
570 580 590 600
....*....|....*....|....*....|....*....|....*
gi 242061796 641 MTVRNQIGAFAAPDKIHWAPGLPKTRSGKIMRRILRKIAARQLDE 685
Cdd:PRK05605 529 AYCREHLTRYKVPRRFYHVDELPRDQLGKVRRREVREELLEKLGA 573
|
|
| PLN02574 |
PLN02574 |
4-coumarate--CoA ligase-like |
162-678 |
1.78e-25 |
|
4-coumarate--CoA ligase-like
Pssm-ID: 215312 [Multi-domain] Cd Length: 560 Bit Score: 111.47 E-value: 1.78e-25
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 162 LTYSELLEKVCQLANYL-KSLGVGKGDAVIIYLPMLLELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPKLVITC 240
Cdd:PLN02574 67 ISYSELQPLVKSMAAGLyHVMGVRQGDVVLLLLPNSVYFPVIFLAVLSLGGIVTTMNPSSSLGEIKKRVVDCSVGLAFTS 146
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 241 NAVKRGVKPIflkdivdvalveseknGVSVGLCLTYENQSAMKREDTKWQperdvwwqDVVTKFPTKCDVEWVDAEDPLF 320
Cdd:PLN02574 147 PENVEKLSPL----------------GVPVIGVPENYDFDSKRIEFPKFY--------ELIKEDFDFVPKPVIKQDDVAA 202
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 321 LLYTSGSTGKPKGVLHTSGGY--MVYTATTFKYA-FDYKPTD-IYWCTADCGWITGHSYVTYGPLLNGATVLVFEGTpny 396
Cdd:PLN02574 203 IMYSSGTTGASKGVVLTHRNLiaMVELFVRFEASqYEYPGSDnVYLAALPMFHIYGLSLFVVGLLSLGSTIVVMRRF--- 279
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 397 pDSGRCWDIVDKYNVTIFYTAPTLVRSLMRdGTEYVTRYSRKSLRVLGSVGEPINPSAWRWFYNIVgdSRCPISDTWWQT 476
Cdd:PLN02574 280 -DASDMVKVIDRFKVTHFPVVPPILMALTK-KAKGVCGEVLKSLKQVSCGAAPLSGKFIQDFVQTL--PHVDFIQGYGMT 355
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 477 E-----TGGFMITPLpgawpQKPGSATFPFFGVQPVIVD-EKGQEIEGECSGYLCIKKswPGAFRTLYGDHERYETTYFK 550
Cdd:PLN02574 356 EstavgTRGFNTEKL-----SKYSSVGLLAPNMQAKVVDwSTGCLLPPGNCGELWIQG--PGVMKGYLNNPKATQSTIDK 428
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 551 PfaGYYFTGDGCSRDKDGYHWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAAVVGVEHEVKGQGIYAFVTLVDGVP 630
Cdd:PLN02574 429 D--GWLRTGDIAYFDEDGYLYIVDRLKEIIKYKGFQIAPADLEAVLISHPEIIDAAVTAVPDKECGEIPVAFVVRRQGST 506
|
490 500 510 520
....*....|....*....|....*....|....*....|....*...
gi 242061796 631 YSEElrkSLIMTVRNQIGAFAAPDKIHWAPGLPKTRSGKIMRRILRKI 678
Cdd:PLN02574 507 LSQE---AVINYVAKQVAPYKKVRKVVFVQSIPKSPAGKILRRELKRS 551
|
|
| A_NRPS_Bac |
cd17655 |
bacitracin synthetase and related proteins; This family of the adenylation (A) domain of ... |
145-675 |
3.04e-25 |
|
bacitracin synthetase and related proteins; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes bacitracin synthetases 1, 2, and 3 (BA1, also known as ATP-dependent cysteine adenylase or cysteine activase, BA2, also known as ATP-dependent lysine adenylase or lysine activase, and BA3, also known as ATP-dependent isoleucine adenylase or isoleucine activase) in Bacilli. Bacitracin is a mixture of related cyclic peptides used as a polypeptide antibiotic. This family also includes gramicidin synthetase 1 involved in synthesis of the cyclic peptide antibiotic gramicidin S via activation of phenylalanine. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341310 [Multi-domain] Cd Length: 490 Bit Score: 110.11 E-value: 3.04e-25
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 145 DKIAMYWEgnepsqDGKLTYSELLEKVCQLANYLKSLGVGKGDAVIIYLPMLLELPIAMLACARIGAVHSVVFAGFSADS 224
Cdd:cd17655 12 DHTAVVFE------DQTLTYRELNERANQLARTLREKGVGPDTIVGIMAERSLEMIVGILGILKAGGAYLPIDPDYPEER 85
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 225 LAQRIVDCKPKLVITCNAVKRGVKPIflkdivdvalvesekngvsvGLCLTYENQSAMKREDTKWQPErdvwwqdvvtkf 304
Cdd:cd17655 86 IQYILEDSGADILLTQSHLQPPIAFI--------------------GLIDLLDEDTIYHEESENLEPV------------ 133
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 305 ptkcdvewVDAEDPLFLLYTSGSTGKPKGV---------LHTSGGYMVYTATTFK------YAFDYKPTDIYwctadcgw 369
Cdd:cd17655 134 --------SKSDDLAYVIYTSGSTGKPKGVmiehrgvvnLVEWANKVIYQGEHLRvalfasISFDASVTEIF-------- 197
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 370 itghsyvtyGPLLNGATVLVfegtpnYPDSGRcWDIV------DKYNVTIFYTAPTLVRSL--MRDGTEYvtrysrkSLR 441
Cdd:cd17655 198 ---------ASLLSGNTLYI------VRKETV-LDGQaltqyiRQNRITIIDLTPAHLKLLdaADDSEGL-------SLK 254
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 442 VLGSVGEPINPSAWRWFYNIVGDSrCPISDTWWQTETG-GFMITPLPGAWPQKP----GSatfPFFGVQPVIVDEKGQEI 516
Cdd:cd17655 255 HLIVGGEALSTELAKKIIELFGTN-PTITNAYGPTETTvDASIYQYEPETDQQVsvpiGK---PLGNTRIYILDQYGRPQ 330
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 517 EGECSGYLCIkkSWPGAFRtlyGDHERYETTYFK----PFAG---YYFTGDGCSRDKDGYHWLTGRVDDVINVSGHRIGT 589
Cdd:cd17655 331 PVGVAGELYI--GGEGVAR---GYLNRPELTAEKfvddPFVPgerMYRTGDLARWLPDGNIEFLGRIDHQVKIRGYRIEL 405
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 590 AEVESALVSHPQCAEAAVVGVEHEVKGQGIYAFVTLVDGVPySEELRKSLimtvRNQIGAFAAP------DKIhwapglP 663
Cdd:cd17655 406 GEIEARLLQHPDIKEAVVIARKDEQGQNYLCAYIVSEKELP-VAQLREFL----ARELPDYMIPsyfiklDEI------P 474
|
570
....*....|..
gi 242061796 664 KTRSGKIMRRIL 675
Cdd:cd17655 475 LTPNGKVDRKAL 486
|
|
| PRK12406 |
PRK12406 |
long-chain-fatty-acid--CoA ligase; Provisional |
157-676 |
7.40e-25 |
|
long-chain-fatty-acid--CoA ligase; Provisional
Pssm-ID: 183506 [Multi-domain] Cd Length: 509 Bit Score: 109.02 E-value: 7.40e-25
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 157 SQDGKLTYSELLEKVCQLANYLKSLGVGKGDAVIIYLPMLLELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPKL 236
Cdd:PRK12406 7 SGDRRRSFDELAQRAARAAGGLAALGVRPGDCVALLMRNDFAFFEAAYAAMRLGAYAVPVNWHFKPEEIAYILEDSGARV 86
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 237 VITCNAVKRGVKPIFLKDiVDVALVESEKngvsvglcltyENQSAMKREDTKWQ-PERDVWWQDVVTKFPTkcdVEWVDA 315
Cdd:PRK12406 87 LIAHADLLHGLASALPAG-VTVLSVPTPP-----------EIAAAYRISPALLTpPAGAIDWEGWLAQQEP---YDGPPV 151
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 316 EDPLFLLYTSGSTGKPKGVLHTSGgymvyTATTFKYAFDYKPTdIYwctadcGWITGHSYVTYGPLLNGA---------- 385
Cdd:PRK12406 152 PQPQSMIYTSGTTGHPKGVRRAAP-----TPEQAAAAEQMRAL-IY------GLKPGIRALLTGPLYHSApnayglragr 219
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 386 --TVLVFEgtPNYpDSGRCWDIVDKYNVTIFYTAPTLVRSLMRDGTEYVTRYSRKSLRVLGSVGEPINPSAWR----WFY 459
Cdd:PRK12406 220 lgGVLVLQ--PRF-DPEELLQLIERHRITHMHMVPTMFIRLLKLPEEVRAKYDVSSLRHVIHAAAPCPADVKRamieWWG 296
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 460 NIvgdsrcpISDTWWQTETGGfMITPLPGAWPQKPGSATFPFFGVQPVIVDEKGQEIE-GECSGYLCIKKSWPGaFRTLY 538
Cdd:PRK12406 297 PV-------IYEYYGSTESGA-VTFATSEDALSHPGTVGKAAPGAELRFVDEDGRPLPqGEIGEIYSRIAGNPD-FTYHN 367
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 539 GDHERYETTYfkpfAGYYFTGDGCSRDKDGYHWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAAVVGVEHEVKGQG 618
Cdd:PRK12406 368 KPEKRAEIDR----GGFITSGDVGYLDADGYLFLCDRKRDMVISGGVNIYPAEIEAVLHAVPGVHDCAVFGIPDAEFGEA 443
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*....
gi 242061796 619 IYAFVTLVDGVPYSEE-LRKSLimtvRNQIGAFAAPDKIHWAPGLPKTRSGKIMRRILR 676
Cdd:PRK12406 444 LMAVVEPQPGATLDEAdIRAQL----KARLAGYKVPKHIEIMAELPREDSGKIFKRRLR 498
|
|
| PRK07786 |
PRK07786 |
long-chain-fatty-acid--CoA ligase; Validated |
162-677 |
1.04e-24 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 169098 [Multi-domain] Cd Length: 542 Bit Score: 108.71 E-value: 1.04e-24
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 162 LTYSELLEKVCQLANYLKSLGVGKGDAVIIYLPMLLELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPKLVITCN 241
Cdd:PRK07786 43 TTWRELDDRVAALAGALSRRGVGFGDRVLILMLNRTEFVESVLAANMLGAIAVPVNFRLTPPEIAFLVSDCGAHVVVTEA 122
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 242 A---VKRGVKPIF-LKDIVDVALVESEKNgvsvglCLTYENQSAmkrEDTKWQPERDVwwqdvvtkfptkcdvewvdAED 317
Cdd:PRK07786 123 AlapVATAVRDIVpLLSTVVVAGGSSDDS------VLGYEDLLA---EAGPAHAPVDI-------------------PND 174
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 318 -PLFLLYTSGSTGKPKG-VL-HTSggyMVYTATTFKYAFDY-KPTDIYWCTADCGWITGHSYVTYGPLLNGATVLvfegt 393
Cdd:PRK07786 175 sPALIMYTSGTTGRPKGaVLtHAN---LTGQAMTCLRTNGAdINSDVGFVGVPLFHIAGIGSMLPGLLLGAPTVI----- 246
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 394 pnYP----DSGRCWDIVDKYNVTIFYTAPTLVRSLMRDGTeyvTRYSRKSLRVLGSVGEPINPSAWRWFYNIVGDSRcpI 469
Cdd:PRK07786 247 --YPlgafDPGQLLDVLEAEKVTGIFLVPAQWQAVCAEQQ---ARPRDLALRVLSWGAAPASDTLLRQMAATFPEAQ--I 319
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 470 SDTWWQTETGGFMITPLPGAWPQKPGSATFPFFGVQPVIVDEKGQEIE----GEcsgylcIKKSWPgafrTLYGDHERYE 545
Cdd:PRK07786 320 LAAFGQTEMSPVTCMLLGEDAIRKLGSVGKVIPTVAARVVDENMNDVPvgevGE------IVYRAP----TLMSGYWNNP 389
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 546 TTYFKPFAGYYF-TGDGCSRDKDGYHWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAAVVGVEHEVKGQGIYAFVT 624
Cdd:PRK07786 390 EATAEAFAGGWFhSGDLVRQDEEGYVWVVDRKKDMIISGGENIYCAEVENVLASHPDIVEVAVIGRADEKWGEVPVAVAA 469
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....
gi 242061796 625 LVDGvpySEELR-KSLIMTVRNQIGAFAAPDKIHWAPGLPKTRSGKIMRRILRK 677
Cdd:PRK07786 470 VRND---DAALTlEDLAEFLTDRLARYKHPKALEIVDALPRNPAGKVLKTELRE 520
|
|
| PRK06145 |
PRK06145 |
acyl-CoA synthetase; Validated |
143-676 |
1.20e-24 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 102207 [Multi-domain] Cd Length: 497 Bit Score: 108.05 E-value: 1.20e-24
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 143 NGDKIAMYWEGNEpsqdgkLTYSELLEKVCQLANYLKSLGVGKGDAVIIYL---PMLLELpiaMLACARIGAVHSVVFAG 219
Cdd:PRK06145 15 TPDRAALVYRDQE------ISYAEFHQRILQAAGMLHARGIGQGDVVALLMknsAAFLEL---AFAASYLGAVFLPINYR 85
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 220 FSADSLAQRIVDCKPKLvitcnavkrgvkpiflkdivdvaLVESEKNGVSVGLCLTYENQSAMKREDTKwqperdvwwqd 299
Cdd:PRK06145 86 LAADEVAYILGDAGAKL-----------------------LLVDEEFDAIVALETPKIVIDAAAQADSR----------- 131
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 300 VVTKFPTKCDVEWVDAEDPLF-LLYTSGSTGKPKGVLHTSGgymvytattfkyafdykptDIYWCTAD----CGWITGHS 374
Cdd:PRK06145 132 RLAQGGLEIPPQAAVAPTDLVrLMYTSGTTDRPKGVMHSYG-------------------NLHWKSIDhviaLGLTASER 192
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 375 YVTYGPL-------LNGATVLVFEGT----PNYpDSGRCWDIVDKYNVTIFYTAPTLVRSLMRDGTEYvtRYSRKSLRVL 443
Cdd:PRK06145 193 LLVVGPLyhvgafdLPGIAVLWVGGTlrihREF-DPEAVLAAIERHRLTCAWMAPVMLSRVLTVPDRD--RFDLDSLAWC 269
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 444 GSVGEPINPSAWRWFYNIVGDSRcpISDTWWQTETGGFMITPLPGAWPQKPGSATFPFFGVQPVIVDEKGQEIEGECSGY 523
Cdd:PRK06145 270 IGGGEKTPESRIRDFTRVFTRAR--YIDAYGLTETCSGDTLMEAGREIEKIGSTGRALAHVEIRIADGAGRWLPPNMKGE 347
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 524 LCIKKswPGAFRTLYGDHERYETTYFkpfAGYYFTGDGCSRDKDGYHWLTGRVDDVINVSGHRIGTAEVESALVSHPQCA 603
Cdd:PRK06145 348 ICMRG--PKVTKGYWKDPEKTAEAFY---GDWFRSGDVGYLDEEGFLYLTDRKKDMIISGGENIASSEVERVIYELPEVA 422
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 242061796 604 EAAVVGVEHEVKGQGIYAFVTLVDGVPYS-EELRKSlimtVRNQIGAFAAPDKIHWAPGLPKTRSGKIMRRILR 676
Cdd:PRK06145 423 EAAVIGVHDDRWGERITAVVVLNPGATLTlEALDRH----CRQRLASFKVPRQLKVRDELPRNPSGKVLKRVLR 492
|
|
| FAA1 |
COG1022 |
Long-chain acyl-CoA synthetase (AMP-forming) [Lipid transport and metabolism]; |
143-609 |
1.27e-24 |
|
Long-chain acyl-CoA synthetase (AMP-forming) [Lipid transport and metabolism];
Pssm-ID: 440645 [Multi-domain] Cd Length: 603 Bit Score: 109.03 E-value: 1.27e-24
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 143 NGDKIAMYWEGNEPSQDgkLTYSELLEKVCQLANYLKSLGVGKGDAVIIYLPMLLELPIAMLACARIGAVHSVVFAGFSA 222
Cdd:COG1022 24 FPDRVALREKEDGIWQS--LTWAEFAERVRALAAGLLALGVKPGDRVAILSDNRPEWVIADLAILAAGAVTVPIYPTSSA 101
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 223 DSLAQRIVDCKPKLVITCNA--VKRgVKPI-----FLKDIVdvalVESEKNGVSVGLCLTYENqsAMKREDTKWQPER-D 294
Cdd:COG1022 102 EEVAYILNDSGAKVLFVEDQeqLDK-LLEVrdelpSLRHIV----VLDPRGLRDDPRLLSLDE--LLALGREVADPAElE 174
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 295 VWWQDvvtkfptkcdvewVDAEDPLFLLYTSGSTGKPKGVLHTSGGyMVYTATTFKYAFDYKPTDIY-----WCtadcgW 369
Cdd:COG1022 175 ARRAA-------------VKPDDLATIIYTSGTTGRPKGVMLTHRN-LLSNARALLERLPLGPGDRTlsflpLA-----H 235
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 370 ITGHSyVTYGPLLNGATVlvfegtpNYPDSGRcwDIVD---KYNVTIF----------YT--------APTLVRSLMRDG 428
Cdd:COG1022 236 VFERT-VSYYALAAGATV-------AFAESPD--TLAEdlrEVKPTFMlavprvwekvYAgiqakaeeAGGLKRKLFRWA 305
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 429 TEYVTRYSRKS--------------------------------LRVLGSVGEPINPSAWRWFYNI-VgdsrcPISDTWWQ 475
Cdd:COG1022 306 LAVGRRYARARlagkspslllrlkhaladklvfsklrealggrLRFAVSGGAALGPELARFFRALgI-----PVLEGYGL 380
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 476 TETGGFMITPLPGAwpQKPGSATFPFFGVQpVIVDEKGqEIE--GEC--SGYlcikkswpgafrtlYGDHEryETTyfKP 551
Cdd:COG1022 381 TETSPVITVNRPGD--NRIGTVGPPLPGVE-VKIAEDG-EILvrGPNvmKGY--------------YKNPE--ATA--EA 438
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|..
gi 242061796 552 FA--GYYFTGD-GcSRDKDGYHWLTGRVDDVINVS-GHRIGTAEVESALVSHPQCAEAAVVG 609
Cdd:COG1022 439 FDadGWLHTGDiG-ELDEDGFLRITGRKKDLIVTSgGKNVAPQPIENALKASPLIEQAVVVG 499
|
|
| OSB_CoA_lg |
cd05912 |
O-succinylbenzoate-CoA ligase (also known as O-succinylbenzoate-CoA synthase, OSB-CoA ... |
313-677 |
1.63e-24 |
|
O-succinylbenzoate-CoA ligase (also known as O-succinylbenzoate-CoA synthase, OSB-CoA synthetase, or MenE); O-succinylbenzoic acid-CoA synthase catalyzes the coenzyme A (CoA)- and ATP-dependent conversion of o-succinylbenzoic acid to o-succinylbenzoyl-CoA. The reaction is the fourth step of the biosynthesis pathway of menaquinone (vitamin K2). In certain bacteria, menaquinone is used during fumarate reduction in anaerobic respiration. In cyanobacteria, the product of the menaquinone pathway is phylloquinone (2-methyl-3-phytyl-1,4-naphthoquinone), a molecule used exclusively as an electron transfer cofactor in Photosystem 1. In green sulfur bacteria and heliobacteria, menaquinones are used as loosely bound secondary electron acceptors in the photosynthetic reaction center.
Pssm-ID: 341238 [Multi-domain] Cd Length: 411 Bit Score: 106.66 E-value: 1.63e-24
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 313 VDAEDPLFLLYTSGSTGKPKGVLHTSGGYMvYTATTFKYAFDYKPTDIYWCTADCGWITGHSYVTYGpLLNGATVLVfeg 392
Cdd:cd05912 74 VKLDDIATIMYTSGTTGKPKGVQQTFGNHW-WSAIGSALNLGLTEDDNWLCALPLFHISGLSILMRS-VIYGMTVYL--- 148
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 393 tpnypdsgrcwdiVDKYN------------VTIFYTAPTLVRSLMRDGTEyvtRYSrKSLR--VLGsvGEPINPSAWRWF 458
Cdd:cd05912 149 -------------VDKFDaeqvlhlinsgkVTIISVVPTMLQRLLEILGE---GYP-NNLRciLLG--GGPAPKPLLEQC 209
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 459 --YNIvgdsrcPISDTWWQTETGGFMITPLPGAWPQKPGSATFPFFGVQPVIVDEKGQEIE-GE--------CSGYLCIK 527
Cdd:cd05912 210 keKGI------PVYQSYGMTETCSQIVTLSPEDALNKIGSAGKPLFPVELKIEDDGQPPYEvGEillkgpnvTKGYLNRP 283
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 528 KSWPGAFrtlygdheryETTYFKpfagyyfTGDGCSRDKDGYHWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAAV 607
Cdd:cd05912 284 DATEESF----------ENGWFK-------TGDIGYLDEEGFLYVLDRRSDLIISGGENIYPAEIEEVLLSHPAIKEAGV 346
|
330 340 350 360 370 380 390
....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 608 VGVEHEVKGQGIYAFVtlVDGVPYSEElrkSLIMTVRNQIGAFAAPDKIHWAPGLPKTRSGKIMRRILRK 677
Cdd:cd05912 347 VGIPDDKWGQVPVAFV--VSERPISEE---ELIAYCSEKLAKYKVPKKIYFVDELPRTASGKLLRHELKQ 411
|
|
| AAS_C |
cd05909 |
C-terminal domain of the acyl-acyl carrier protein synthetase (also called ... |
160-679 |
5.65e-24 |
|
C-terminal domain of the acyl-acyl carrier protein synthetase (also called 2-acylglycerophosphoethanolamine acyltransferase, Aas); Acyl-acyl carrier protein synthase (Aas) is a membrane protein responsible for a minor pathway of incorporating exogenous fatty acids into membrane phospholipids. Its in vitro activity is characterized by the ligation of free fatty acids between 8 and 18 carbons in length to the acyl carrier protein sulfydryl group (ACP-SH) in the presence of ATP and Mg2+. However, its in vivo function is as a 2-acylglycerophosphoethanolamine (2-acyl-GPE) acyltransferase. The reaction occurs in two steps: the acyl chain is first esterified to acyl carrier protein (ACP) via a thioester bond, followed by a second step where the acyl chain is transferred to a 2-acyllysophospholipid, thus completing the transacylation reaction. This model represents the C-terminal domain of the enzyme, which belongs to the class I adenylate-forming enzyme family, including acyl-CoA synthetases.
Pssm-ID: 341235 [Multi-domain] Cd Length: 490 Bit Score: 105.88 E-value: 5.65e-24
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 160 GKLTYSELLEKVCQLANYLKSLGVGkGDAVIIYLPMLLELPIAMLACARIGAVhsVVFAGFSADSLAQR--IVDCKPKLV 237
Cdd:cd05909 6 TSLTYRKLLTGAIALARKLAKMTKE-GENVGVMLPPSAGGALANFALALSGKV--PVMLNYTAGLRELRacIKLAGIKTV 82
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 238 ITCNAV--KRGVKPIFLKDI-VDVALVESEKNGVSvglcltyenqsamkredtkwqperdvWWQDVVTKFPTKCDVEW-- 312
Cdd:cd05909 83 LTSKQFieKLKLHHLFDVEYdARIVYLEDLRAKIS--------------------------KADKCKAFLAGKFPPKWll 136
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 313 -------VDAEDPLFLLYTSGSTGKPKGVLHTSGGYM--VYTATTFkyaFDYKPTDIYwctadCGWITG-HSY----VTY 378
Cdd:cd05909 137 rifgvapVQPDDPAVILFTSGSEGLPKGVVLSHKNLLanVEQITAI---FDPNPEDVV-----FGALPFfHSFgltgCLW 208
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 379 GPLLNGATVlVFEgtPNYPDSGRCWDIVDKYNVTIFYTAPTLVRSLMRdgteYVTRYSRKSLRVLGSVGEPINPSAWRWF 458
Cdd:cd05909 209 LPLLSGIKV-VFH--PNPLDYKKIPELIYDKKATILLGTPTFLRGYAR----AAHPEDFSSLRLVVAGAEKLKDTLRQEF 281
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 459 YNIVGdsrCPISDTWWQTETGGFMITPLPGAwPQKPGSATFPFFGVQPVIVDEKGQE--IEGEcSGYLCIKKswPGAFRT 536
Cdd:cd05909 282 QEKFG---IRILEGYGTTECSPVISVNTPQS-PNKEGTVGRPLPGMEVKIVSVETHEevPIGE-GGLLLVRG--PNVMLG 354
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 537 LYGDHERyetTYFKPFAGYYFTGDGCSRDKDGYHWLTGRVDDVINVSGHRIGTAEVESALVSH-PQCAEAAVVGVEHEVK 615
Cdd:cd05909 355 YLNEPEL---TSFAFGDGWYDTGDIGKIDGEGFLTITGRLSRFAKIAGEMVSLEAIEDILSEIlPEDNEVAVVSVPDGRK 431
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 242061796 616 GQGIYAFVTLVDGVPYS--EELRKSlimtvrnQIGAFAAPDKIHWAPGLPKTRSGKIMRRILRKIA 679
Cdd:cd05909 432 GEKIVLLTTTTDTDPSSlnDILKNA-------GISNLAKPSYIHQVEEIPLLGTGKPDYVTLKALA 490
|
|
| OSB_MenE-like |
cd17630 |
O-succinylbenzoic acid-CoA ligase; This family contains O-succinylbenzoyl-CoA (OSB-CoA) ... |
317-679 |
1.64e-23 |
|
O-succinylbenzoic acid-CoA ligase; This family contains O-succinylbenzoyl-CoA (OSB-CoA) synthetase (also known as O-succinylbenzoic acid CoA ligase) that belongs to the ANL superfamily and catalyzes the ligation of CoA to o-succinylbenzoate (OSB). It includes MenE in the bacterial menaquinone biosynthesis pathway which is a promising target for the development of novel antibacterial agents. MenE catalyzes CoA ligation via an acyl-adenylate intermediate; tight-binding inhibitors of MenE based on stable acyl-sulfonyladenosine analogs of this intermediate provide a pathway toward the development of optimized MenE inhibitors.
Pssm-ID: 341285 [Multi-domain] Cd Length: 325 Bit Score: 102.02 E-value: 1.64e-23
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 317 DPLFLLYTSGSTGKPKGVLHTSGGyMVYTATTFKYAFDYKPTDIYWCTADCGWITGHSYVTYGPLLNGATVLVFEGTPNY 396
Cdd:cd17630 1 RLATVILTSGSTGTPKAVVHTAAN-LLASAAGLHSRLGFGGGDSWLLSLPLYHVGGLAILVRSLLAGAELVLLERNQALA 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 397 PDSGRcwdivdkYNVTIFYTAPTLVRSLMRDGTeyvTRYSRKSLRVLGSVGEPINPSAWRWFynivGDSRCPISDTWWQT 476
Cdd:cd17630 80 EDLAP-------PGVTHVSLVPTQLQRLLDSGQ---GPAALKSLRAVLLGGAPIPPELLERA----ADRGIPLYTTYGMT 145
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 477 ETGGfMITPLPGAWPQKPGSATfPFFGVQPVIVDEkgqeiegecsGYLCIKkswpGAfrTLYGDHERYETTYFKPFAGYY 556
Cdd:cd17630 146 ETAS-QVATKRPDGFGRGGVGV-LLPGRELRIVED----------GEIWVG----GA--SLAMGYLRGQLVPEFNEDGWF 207
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 557 FTGDGCSRDKDGYHWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAAVVGVEHEVKGQGIYAFVTLVDGVPYSEelr 636
Cdd:cd17630 208 TTKDLGELHADGRLTVLGRADNMIISGGENIQPEEIEAALAAHPAVRDAFVVGVPDEELGQRPVAVIVGRGPADPAE--- 284
|
330 340 350 360
....*....|....*....|....*....|....*....|...
gi 242061796 637 ksLIMTVRNQIGAFAAPDKIHWAPGLPKTRSGKIMRRILRKIA 679
Cdd:cd17630 285 --LRAWLKDKLARFKLPKRIYPVPELPRTGGGKVDRRALRAWL 325
|
|
| PRK06164 |
PRK06164 |
acyl-CoA synthetase; Validated |
158-685 |
1.71e-23 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 235722 [Multi-domain] Cd Length: 540 Bit Score: 105.21 E-value: 1.71e-23
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 158 QDGKLTYSELLEKVCQLANYLKSLGVGKGDAVIIYLPMLLELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPKLV 237
Cdd:PRK06164 32 EDRPLSRAELRALVDRLAAWLAAQGVRRGDRVAVWLPNCIEWVVLFLACARLGATVIAVNTRYRSHEVAHILGRGRARWL 111
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 238 ITCNAVKR--------GVKPIFLKDIVDVALVESEKngvsvglcltyenqsamkreDTKWQPERDVWWQDVVTKFPTKCD 309
Cdd:PRK06164 112 VVWPGFKGidfaailaAVPPDALPPLRAIAVVDDAA--------------------DATPAPAPGARVQLFALPDPAPPA 171
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 310 VEWVDA--EDPLFLLYT-SGSTGKPKGVLHTSGGyMVYTATTFKYAFDYKPTDIYWCTAD-CGwITGHSYVTyGPLLNGA 385
Cdd:PRK06164 172 AAGERAadPDAGALLFTtSGTTSGPKLVLHRQAT-LLRHARAIARAYGYDPGAVLLAALPfCG-VFGFSTLL-GALAGGA 248
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 386 TVLVFegtPNYpDSGRCWDIVDKYNVTIFYTAPTLVRSLMRDGTEyvtRYSRKSLRVLGSVGepiNPSAWRWFYNIVGDS 465
Cdd:PRK06164 249 PLVCE---PVF-DAARTARALRRHRVTHTFGNDEMLRRILDTAGE---RADFPSARLFGFAS---FAPALGELAALARAR 318
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 466 RCPISDTWWQTE-----TGGFMITP-----LPGAWPQKPGsatfpffgVQPVIVDEKGQEI--EGEcSGYLCIKKswPGA 533
Cdd:PRK06164 319 GVPLTGLYGSSEvqalvALQPATDPvsvriEGGGRPASPE--------ARVRARDPQDGALlpDGE-SGEIEIRA--PSL 387
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 534 FRTlYGDHERYETTYFKPfAGYYFTGD-GCSRDKDGYHWLTgRVDDVINVSGHRIGTAEVESALVSHPQCAEAAVVGVEH 612
Cdd:PRK06164 388 MRG-YLDNPDATARALTD-DGYFRTGDlGYTRGDGQFVYQT-RMGDSLRLGGFLVNPAEIEHALEALPGVAAAQVVGATR 464
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 242061796 613 EvkGQGI-YAFVTLVDGVPYSEElrkSLIMTVRNQIGAFAAPDKIHWAPGLPKTRSG---KIMRRILRKIAARQLDE 685
Cdd:PRK06164 465 D--GKTVpVAFVIPTDGASPDEA---GLMAACREALAGFKVPARVQVVEAFPVTESAngaKIQKHRLREMAQARLAA 536
|
|
| PRK08162 |
PRK08162 |
acyl-CoA synthetase; Validated |
163-688 |
2.47e-23 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236169 [Multi-domain] Cd Length: 545 Bit Score: 104.64 E-value: 2.47e-23
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 163 TYSELLEKVCQLANYLKSLGVGKGDAVIIYLPMLLELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPKLVIT--- 239
Cdd:PRK08162 45 TWAETYARCRRLASALARRGIGRGDTVAVLLPNIPAMVEAHFGVPMAGAVLNTLNTRLDAASIAFMLRHGEAKVLIVdte 124
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 240 -CNAVKR-----GVKPIFLKDIVDVALVESEKNGVsvglcLTYENQSAMKREDTKWQPERDvwwqdvvtkfptkcdvEWv 313
Cdd:PRK08162 125 fAEVAREalallPGPKPLVIDVDDPEYPGGRFIGA-----LDYEAFLASGDPDFAWTLPAD----------------EW- 182
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 314 daeDPLFLLYTSGSTGKPKGVL-HTSGGYMVYTATTFkyAFDYKPTDIY-----------WCTAdcgWitghsyvtygpl 381
Cdd:PRK08162 183 ---DAIALNYTSGTTGNPKGVVyHHRGAYLNALSNIL--AWGMPKHPVYlwtlpmfhcngWCFP---W------------ 242
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 382 lngaTVLVFEGTP---NYPDSGRCWDIVDKYNVTIFYTAPtLVRSLMRDGTEYVTRYSRKSLRVLGSVGEPinPSAwrwf 458
Cdd:PRK08162 243 ----TVAARAGTNvclRKVDPKLIFDLIREHGVTHYCGAP-IVLSALINAPAEWRAGIDHPVHAMVAGAAP--PAA---- 311
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 459 ynIVGdsrcpisdtwwQTETGGFMITPLPG-----------AWpqKPGSATFPFF---------GV-------------- 504
Cdd:PRK08162 312 --VIA-----------KMEEIGFDLTHVYGltetygpatvcAW--QPEWDALPLDeraqlkarqGVryplqegvtvldpd 376
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 505 --QPVIVDekGQEIeGEC--------SGYLCIKKSWPGAFRtlygdheryettyfkpfAGYYFTGDGCSRDKDGYHWLTG 574
Cdd:PRK08162 377 tmQPVPAD--GETI-GEImfrgnivmKGYLKNPKATEEAFA-----------------GGWFHTGDLAVLHPDGYIKIKD 436
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 575 RVDDVINVSGHRIGTAEVESALVSHPQCAEAAVVGVEHEVKGQGIYAFVTLVDGVPYSEElrkSLIMTVRNQIGAFAAPD 654
Cdd:PRK08162 437 RSKDIIISGGENISSIEVEDVLYRHPAVLVAAVVAKPDPKWGEVPCAFVELKDGASATEE---EIIAHCREHLAGFKVPK 513
|
570 580 590
....*....|....*....|....*....|....
gi 242061796 655 KIHWAPgLPKTRSGKIMRRILRKiAARQLDELGD 688
Cdd:PRK08162 514 AVVFGE-LPKTSTGKIQKFVLRE-QAKSLKAIDL 545
|
|
| PRK12467 |
PRK12467 |
peptide synthase; Provisional |
159-685 |
4.74e-23 |
|
peptide synthase; Provisional
Pssm-ID: 237108 [Multi-domain] Cd Length: 3956 Bit Score: 105.63 E-value: 4.74e-23
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 159 DGKLTYSELLEKVCQLANYLKSLGVGKGDAVIIYLPMLLELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPKLVI 238
Cdd:PRK12467 3118 DQQLSYAELNRRANRLAHRLIAIGVGPDVLVGVAVERSVEMIVALLAVLKAGGAYVPLDPEYPRERLAYMIEDSGVKLLL 3197
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 239 TCNAVkrgvkpiflkdivdvalveSEKNGVSVGlcltyenqsamkreDTKWQPERDVWWqdvvtKFPTKCDVEWVDAEDP 318
Cdd:PRK12467 3198 TQAHL-------------------LEQLPAPAG--------------DTALTLDRLDLN-----GYSENNPSTRVMGENL 3239
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 319 LFLLYTSGSTGKPKGV----------LHTSGGYMVYTATT-----FKYAFDYKPTDIYWctadcgwitghsyvtygPLLN 383
Cdd:PRK12467 3240 AYVIYTSGSTGKPKGVgvrhgalanhLCWIAEAYELDANDrvllfMSFSFDGAQERFLW-----------------TLIC 3302
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 384 GATVLVFEGtpNYPDSGRCWDIVDKYNVTIFYTAPTLVRSLMRDGteyvTRYSRKSLRVLGSVGEPINPSAW-------- 455
Cdd:PRK12467 3303 GGCLVVRDN--DLWDPEELWQAIHAHRISIACFPPAYLQQFAEDA----GGADCASLDIYVFGGEAVPPAAFeqvkrklk 3376
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 456 -RWFYNIVGDSRCPISDTWWQTETGGfmiTPLPGAWP---QKPGSATFPFFG-VQPVIVDEKGQ-EIEGECSGYlcikks 529
Cdd:PRK12467 3377 pRGLTNGYGPTEAVVTVTLWKCGGDA---VCEAPYAPigrPVAGRSIYVLDGqLNPVPVGVAGElYIGGVGLAR------ 3447
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 530 wpGAFRTLYGDHERYETTyfkPFAG----YYFTGDGCSRDKDGYHWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEA 605
Cdd:PRK12467 3448 --GYHQRPSLTAERFVAD---PFSGsggrLYRTGDLARYRADGVIEYLGRIDHQVKIRGFRIELGEIEARLLQHPSVREA 3522
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 606 AVVGVEHEvKGQGIYAFVTLVDgvpYSEELRKSLIMTVRNQIGAFAAPDKIHWAPGLPKTRSGKIMRRILRKIAARQLDE 685
Cdd:PRK12467 3523 VVLARDGA-GGKQLVAYVVPAD---PQGDWRETLRDHLAASLPDYMVPAQLLVLAAMPLGPNGKVDRKALPDPDAKGSRE 3598
|
|
| ttLC_FACS_like |
cd05915 |
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles; This family includes ... |
153-677 |
4.78e-23 |
|
Fatty acyl-CoA synthetases similar to LC-FACS from Thermus thermophiles; This family includes fatty acyl-CoA synthetases that can activate medium-chain to long-chain fatty acids. They catalyze the ATP-dependent acylation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. Fatty acyl-CoA synthetases are responsible for fatty acid degradation as well as physiological regulation of cellular functions via the production of fatty acyl-CoA esters. The fatty acyl-CoA synthetase from Thermus thermophiles in this family has been shown to catalyze the long-chain fatty acid, myristoyl acid, while another member in this family, the AlkK protein identified in Pseudomonas oleovorans, targets medium chain fatty acids. This family also includes an uncharacterized subgroup of FACS.
Pssm-ID: 213283 [Multi-domain] Cd Length: 509 Bit Score: 103.28 E-value: 4.78e-23
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 153 GNEPSQDGKLTYSELLEKVCQLANYLKSLGVGKGDAVIIYLPMLLELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDC 232
Cdd:cd05915 16 RLHTGEVHRTTYAEVYQRARRLMGGLRALGVGVGDRVATLGFNHFRHLEAYFAVPGMGAVLHTANPRLSPKEIAYILNHA 95
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 233 KPKlvitcnavkrgvkpiflkdivdVALVESEKNGVSVGLCLTYENQSAMKREDTKWQPERDVwwqdVVTKFPTKCDVEW 312
Cdd:cd05915 96 EDK----------------------VLLFDPNLLPLVEAIRGELKTVQHFVVMDEKAPEGYLA----YEEALGEEADPVR 149
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 313 VDAEDPLFLLYTSGSTGKPKGVLHT-SGGYMVYTATTFKYAFDYKPTDIYWCTADCGWITGHSYVTYGPLLNGATVLVFE 391
Cdd:cd05915 150 VPERAACGMAYTTGTTGLPKGVVYShRALVLHSLAASLVDGTALSEKDVVLPVVPMFHVNAWCLPYAATLVGAKQVLPGP 229
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 392 gtpnYPDSGRCWDIVDKYNVTIFYTAPTlVRSLMRDGTEYVTRYSRKSLRVLGSVGEPinPSAWRWFYNIvGDSRCPISD 471
Cdd:cd05915 230 ----RLDPASLVELFDGEGVTFTAGVPT-VWLALADYLESTGHRLKTLRRLVVGGSAA--PRSLIARFER-MGVEVRQGY 301
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 472 TWWQTETGGFMITPLPgAWPQKPGSATFPF--------FGVQPVIVDEKGQEIEGECSGYLCIKKSWPGAFRTLYGDHER 543
Cdd:cd05915 302 GLTETSPVVVQNFVKS-HLESLSEEEKLTLkaktglpiPLVRLRVADEEGRPVPKDGKALGEVQLKGPWITGGYYGNEEA 380
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 544 YETTYFKpfAGYYFTGDGCSRDKDGYHWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAAVVGVEHEVKGQGIYAFV 623
Cdd:cd05915 381 TRSALTP--DGFFRTGDIAVWDEEGYVEIKDRLKDLIKSGGEWISSVDLENALMGHPKVKEAAVVAIPHPKWQERPLAVV 458
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*
gi 242061796 624 TLVDGVPYSEElrksLIMTVRNQIGAFAA-PDKIHWAPGLPKTRSGKIMRRILRK 677
Cdd:cd05915 459 VPRGEKPTPEE----LNEHLLKAGFAKWQlPDAYVFAEEIPRTSAGKFLKRALRE 509
|
|
| A_NRPS_ApnA-like |
cd17644 |
similar to adenylation domain of anabaenopeptin synthetase (ApnA); This family of the ... |
143-675 |
7.26e-23 |
|
similar to adenylation domain of anabaenopeptin synthetase (ApnA); This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes Planktothrix agardhii anabaenopeptin synthetase (ApnA A1), which is capable of activating two chemically distinct amino acids (Arg and Tyr). Structural studies show that the architecture of the active site forces Arg to adopt a Tyr-like conformation, thus explaining the bispecificity. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341299 [Multi-domain] Cd Length: 465 Bit Score: 102.51 E-value: 7.26e-23
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 143 NGDKIAMYWEgnepsqDGKLTYSELLEKVCQLANYLKSLGVGKGDAVIIYLPMLLELPIAMLACARIGAVHSVVFAGFSA 222
Cdd:cd17644 13 TPDAVAVVFE------DQQLTYEELNTKANQLAHYLQSLGVKSESLVGICVERSLEMIIGLLAILKAGGAYVPLDPNYPQ 86
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 223 DSLAQRIVDCKPKLVITcnavkrgvkpiflkdivdvalvesekngvsvglcltyenqsamkredtkwQPErdvwwqdvvt 302
Cdd:cd17644 87 ERLTYILEDAQISVLLT--------------------------------------------------QPE---------- 106
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 303 kfptkcdvewvdaeDPLFLLYTSGSTGKPKGVL--------HTSGGYMVYTATT-------FKYAFDYKPTDIYWCtadc 367
Cdd:cd17644 107 --------------NLAYVIYTSGSTGKPKGVMiehqslvnLSHGLIKEYGITSsdrvlqfASIAFDVAAEEIYVT---- 168
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 368 gWITGHSyvtygplLNGATVLVFegtpnyPDSGRCWDIVDKYNVTIFYTAPTLVRSLMRDGTEyVTRYSRKSLRVLGSVG 447
Cdd:cd17644 169 -LLSGAT-------LVLRPEEMR------SSLEDFVQYIQQWQLTVLSLPPAYWHLLVLELLL-STIDLPSSLRLVIVGG 233
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 448 EPINPSAWR-W---------FYNIVGDSRCPISDTWwqtetggFMITPLPGAWPQKPGSATfPFFGVQPVIVDEKGQEIE 517
Cdd:cd17644 234 EAVQPELVRqWqknvgnfiqLINVYGPTEATIAATV-------CRLTQLTERNITSVPIGR-PIANTQVYILDENLQPVP 305
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 518 GECSGYLCIkkswpGAFRTLYGDHERYETTYFK----PFAG-----YYFTGDGCSRDKDGYHWLTGRVDDVINVSGHRIG 588
Cdd:cd17644 306 VGVPGELHI-----GGVGLARGYLNRPELTAEKfishPFNSseserLYKTGDLARYLPDGNIEYLGRIDNQVKIRGFRIE 380
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 589 TAEVESALVSHPQCAEAAVVGVEHEVKGQGIYAF-VTLVDGVPYSEELRKSLimtvRNQIGAFAAPDKIHWAPGLPKTRS 667
Cdd:cd17644 381 LGEIEAVLSQHNDVKTAVVIVREDQPGNKRLVAYiVPHYEESPSTVELRQFL----KAKLPDYMIPSAFVVLEELPLTPN 456
|
....*...
gi 242061796 668 GKIMRRIL 675
Cdd:cd17644 457 GKIDRRAL 464
|
|
| PRK07787 |
PRK07787 |
acyl-CoA synthetase; Validated |
491-676 |
7.67e-23 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236096 [Multi-domain] Cd Length: 471 Bit Score: 102.38 E-value: 7.67e-23
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 491 PQKPGSATFPFFGVQPVIVDEKGQEI--EGECSGYLCIKKswPGAFRtlyGDHERYETT--YFKPfAGYYFTGDGCSRDK 566
Cdd:PRK07787 289 ERRPGWVGLPLAGVETRLVDEDGGPVphDGETVGELQVRG--PTLFD---GYLNRPDATaaAFTA-DGWFRTGDVAVVDP 362
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 567 DGYHWLTGRVD-DVINVSGHRIGTAEVESALVSHPQCAEAAVVGVEHEVKGQGIYAFVTLVDGVPYSEelrksLIMTVRN 645
Cdd:PRK07787 363 DGMHRIVGREStDLIKSGGYRIGAGEIETALLGHPGVREAAVVGVPDDDLGQRIVAYVVGADDVAADE-----LIDFVAQ 437
|
170 180 190
....*....|....*....|....*....|.
gi 242061796 646 QIGAFAAPDKIHWAPGLPKTRSGKIMRRILR 676
Cdd:PRK07787 438 QLSVHKRPREVRFVDALPRNAMGKVLKKQLL 468
|
|
| PRK12583 |
PRK12583 |
acyl-CoA synthetase; Provisional |
158-683 |
1.18e-22 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 237145 [Multi-domain] Cd Length: 558 Bit Score: 102.54 E-value: 1.18e-22
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 158 QDGKLTYSELLEKVCQLANYLKSLGVGKGDAVIIYLPMLLELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPKLV 237
Cdd:PRK12583 42 QALRYTWRQLADAVDRLARGLLALGVQPGDRVGIWAPNCAEWLLTQFATARIGAILVNINPAYRASELEYALGQSGVRWV 121
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 238 ITCNAVKRGVKPIFLKDIVDvALVESEKNGVSVGLCLTYENQSAMKREDtkwqPERDVWWQDVVTKFPTKCDVEW----- 312
Cdd:PRK12583 122 ICADAFKTSDYHAMLQELLP-GLAEGQPGALACERLPELRGVVSLAPAP----PPGFLAWHELQARGETVSREALaerqa 196
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 313 -VDAEDPLFLLYTSGSTGKPKGVLHT-----SGGYMVYTATTFKYAfDY--KPTDIYWCTadcgwitGHSYVTYGPLLNG 384
Cdd:PRK12583 197 sLDRDDPINIQYTSGTTGFPKGATLShhnilNNGYFVAESLGLTEH-DRlcVPVPLYHCF-------GMVLANLGCMTVG 268
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 385 ATVLVfegtPN-YPDSGRCWDIVDKYNVTIFYTAPTLVRSLMrDGTEYvTRYSRKSLRVLGSVGEPINPSAWRwfyNIVG 463
Cdd:PRK12583 269 ACLVY----PNeAFDPLATLQAVEEERCTALYGVPTMFIAEL-DHPQR-GNFDLSSLRTGIMAGAPCPIEVMR---RVMD 339
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 464 DSRCP-ISDTWWQTETGGfmITPLPGAWPQKPGSATfPFFGVQP----VIVDEKGQEI----EGE-CS-GYLCIKKSWPG 532
Cdd:PRK12583 340 EMHMAeVQIAYGMTETSP--VSLQTTAADDLERRVE-TVGRTQPhlevKVVDPDGATVprgeIGElCTrGYSVMKGYWNN 416
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 533 AFRTLYGDHERyettyfkpfaGYYFTGDGCSRDKDGYHWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAAVVGVEH 612
Cdd:PRK12583 417 PEATAESIDED----------GWMHTGDLATMDEQGYVRIVGRSKDMIIRGGENIYPREIEEFLFTHPAVADVQVFGVPD 486
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 242061796 613 EVKGQGIYAFVTLVDGVPYS-EELRKslimTVRNQIGAFAAPDKIHWAPGLPKTRSGKIMRRILRKIAARQL 683
Cdd:PRK12583 487 EKYGEEIVAWVRLHPGHAASeEELRE----FCKARIAHFKVPRYFRFVDEFPMTVTGKVQKFRMREISIEEL 554
|
|
| PRK12316 |
PRK12316 |
peptide synthase; Provisional |
162-675 |
1.75e-22 |
|
peptide synthase; Provisional
Pssm-ID: 237054 [Multi-domain] Cd Length: 5163 Bit Score: 103.88 E-value: 1.75e-22
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 162 LTYSELLEKVCQLANYLKSLGVGKGDAVIIYLPMLLELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPKLVITCN 241
Cdd:PRK12316 537 LDYAELNRRANRLAHALIERGVGPDVLVGVAMERSIEMVVALLAILKAGGAYVPLDPEYPAERLAYMLEDSGVQLLLSQS 616
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 242 avkrgvkpiFLKDIVDVAlvesekNGVSVgLCLTyenqsamkredtkwQPerDVWWQDVVTKFPTKCdvewVDAEDPLFL 321
Cdd:PRK12316 617 ---------HLGRKLPLA------AGVQV-LDLD--------------RP--AAWLEGYSEENPGTE----LNPENLAYV 660
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 322 LYTSGSTGKPKGVLHTSG-----------------GYMVYTATTFkyAFDYKPTDIYWctadcgwitghsyvtygPLLNG 384
Cdd:PRK12316 661 IYTSGSTGKPKGAGNRHRalsnrlcwmqqayglgvGDTVLQKTPF--SFDVSVWEFFW-----------------PLMSG 721
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 385 ATvLVFEGTPNYPDSGRCWDIVDKYNVTIFYTAPTLVRSLMRDGTEYvtrySRKSLRVLGSVGEPINPSAW------RW- 457
Cdd:PRK12316 722 AR-LVVAAPGDHRDPAKLVELINREGVDTLHFVPSMLQAFLQDEDVA----SCTSLRRIVCSGEALPADAQeqvfakLPq 796
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 458 --FYNIVGDSRCPISDTWWQTETGGFMITPLpGAwpqkpgsatfPFFGVQPVIVDEKGQEIEGECSGYLCIKKSwpGAFR 535
Cdd:PRK12316 797 agLYNLYGPTEAAIDVTHWTCVEEGGDSVPI-GR----------PIANLACYILDANLEPVPVGVLGELYLAGR--GLAR 863
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 536 TLYG----DHERYETTYFKPFAGYYFTGDGCSRDKDGYHWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAAVVGVE 611
Cdd:PRK12316 864 GYHGrpglTAERFVPSPFVAGERMYRTGDLARYRADGVIEYAGRIDHQVKLRGLRIELGEIEARLLEHPWVREAAVLAVD 943
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 242061796 612 hevkGQGIYAFVTLVDGvpySEELRKSLIMTVRNQIGAFAAPDkiHWAP--GLPKTRSGKIMRRIL 675
Cdd:PRK12316 944 ----GKQLVGYVVLESE---GGDWREALKAHLAASLPEYMVPA--QWLAleRLPLTPNGKLDRKAL 1000
|
|
| PRK12316 |
PRK12316 |
peptide synthase; Provisional |
161-685 |
2.06e-22 |
|
peptide synthase; Provisional
Pssm-ID: 237054 [Multi-domain] Cd Length: 5163 Bit Score: 103.50 E-value: 2.06e-22
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 161 KLTYSELLEKVCQLANYLKSLGVGKGDAVIIYLPMLLELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPKLVITC 240
Cdd:PRK12316 4576 KLTYAELNRRANRLAHALIARGVGPEVLVGIAMERSAEMMVGLLAVLKAGGAYVPLDPEYPRERLAYMMEDSGAALLLTQ 4655
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 241 NAVKRGVkPIflkdivdvalveseKNGVSvglCLTYEnqsamkredtkwqpeRDVWWQDvvtkFPTKCDVEWVDAEDPLF 320
Cdd:PRK12316 4656 SHLLQRL-PI--------------PDGLA---SLALD---------------RDEDWEG----FPAHDPAVRLHPDNLAY 4698
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 321 LLYTSGSTGKPKGVLHTSGGYMVYTATTFKYafdykptdiYWCTADCGWITGHSYV-------TYGPLLNGATVLVfeGT 393
Cdd:PRK12316 4699 VIYTSGSTGRPKGVAVSHGSLVNHLHATGER---------YELTPDDRVLQFMSFSfdgshegLYHPLINGASVVI--RD 4767
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 394 PNYPDSGRCWDIVDKYNVTIFYTAPTLVRSLMRDGTEyvtRYSRKSLRVLGSVGEPINPS----AWR-----WFYNIVGD 464
Cdd:PRK12316 4768 DSLWDPERLYAEIHEHRVTVLVFPPVYLQQLAEHAER---DGEPPSLRVYCFGGEAVAQAsydlAWRalkpvYLFNGYGP 4844
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 465 SRCPISDTWWQTETG---GFMITPLPGAWPqkpGSATFPFFG-VQPVIVDEKGQ-EIEGECSGYlcikkswpGAFRTLYG 539
Cdd:PRK12316 4845 TETTVTVLLWKARDGdacGAAYMPIGTPLG---NRSGYVLDGqLNPLPVGVAGElYLGGEGVAR--------GYLERPAL 4913
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 540 DHERYETTYF-KPFAGYYFTGDGCSRDKDGYHWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAAVVGVEHEVkGQG 618
Cdd:PRK12316 4914 TAERFVPDPFgAPGGRLYRTGDLARYRADGVIDYLGRVDHQVKIRGFRIELGEIEARLREHPAVREAVVIAQEGAV-GKQ 4992
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 242061796 619 IYAFVT-----LVDGVPYSEELRKSLIMTVRNQIGAFAAPDKIHWAPGLPKTRSGKIMRRILRKIAARQLDE 685
Cdd:PRK12316 4993 LVGYVVpqdpaLADADEAQAELRDELKAALRERLPEYMVPAHLVFLARMPLTPNGKLDRKALPQPDASLLQQ 5064
|
|
| A_NRPS_ProA |
cd17656 |
gramicidin S synthase 2, also known as ATP-dependent proline adenylase; This family of the ... |
158-675 |
2.30e-22 |
|
gramicidin S synthase 2, also known as ATP-dependent proline adenylase; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) contains gramicidin S synthase 2 (also known as ATP-dependent proline adenylase or proline activase or ProA). ProA is a multifunctional enzyme involved in synthesis of the cyclic peptide antibiotic gramicidin S and able to activate and polymerize the amino acids proline, valine, ornithine and leucine. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341311 [Multi-domain] Cd Length: 479 Bit Score: 101.01 E-value: 2.30e-22
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 158 QDGKLTYSELLEKVCQLANYLKSLGVGKGDAVIIYLPMLLELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPKLV 237
Cdd:cd17656 10 ENQKLTYRELNERSNQLARFLREKGVKKDSIVAIMMERSAEMIVGILGILKAGGAFVPIDPEYPEERRIYIMLDSGVRVV 89
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 238 ITCNAVKrgVKPIFLKDIV----DVALVESEKNgvsvglcLTYENqsamkredtkwqperdvwwqdvvtkfptkcdvewv 313
Cdd:cd17656 90 LTQRHLK--SKLSFNKSTIlledPSISQEDTSN-------IDYIN----------------------------------- 125
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 314 DAEDPLFLLYTSGSTGKPKGVL--HTSggymvyTATTFKYAFDYKPTDIYwctADCGWITGHSY-VTY----GPLLNGAT 386
Cdd:cd17656 126 NSDDLLYIIYTSGTTGKPKGVQleHKN------MVNLLHFEREKTNINFS---DKVLQFATCSFdVCYqeifSTLLSGGT 196
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 387 V-LVFEGTPNypDSGRCWDIVDKYNVTIFYTaPTLVRSLMRDGTEYVTRYSrKSLRVLGSVGEPI---NPsawrwFYNIV 462
Cdd:cd17656 197 LyIIREETKR--DVEQLFDLVKRHNIEVVFL-PVAFLKFIFSEREFINRFP-TCVKHIITAGEQLvitNE-----FKEML 267
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 463 GDSRCPISDTWWQTET---GGFMITPLPgAWPQKPGSATfPFFGVQPVIVDEKGQEIEGECSGYLCIkkSWPGAFRTLYG 539
Cdd:cd17656 268 HEHNVHLHNHYGPSEThvvTTYTINPEA-EIPELPPIGK-PISNTWIYILDQEQQLQPQGIVGELYI--SGASVARGYLN 343
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 540 D----HERYETTYFKPFAGYYFTGDGCSRDKDGYHWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAAVVGVEHEVK 615
Cdd:cd17656 344 RqeltAEKFFPDPFDPNERMYRTGDLARYLPDGNIEFLGRADHQVKIRGYRIELGEIEAQLLNHPGVSEAVVLDKADDKG 423
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 616 GQGIYAFVTLVDGVPySEELRKSLimtvRNQIGAFAAPDKIHWAPGLPKTRSGKIMRRIL 675
Cdd:cd17656 424 EKYLCAYFVMEQELN-ISQLREYL----AKQLPEYMIPSFFVPLDQLPLTPNGKVDRKAL 478
|
|
| VL_LC_FACS_like |
cd05907 |
Long-chain fatty acid CoA synthetases and Bubblegum-like very long-chain fatty acid CoA ... |
162-609 |
3.09e-22 |
|
Long-chain fatty acid CoA synthetases and Bubblegum-like very long-chain fatty acid CoA synthetases; This family includes long-chain fatty acid (C12-C20) CoA synthetases and Bubblegum-like very long-chain (>C20) fatty acid CoA synthetases. FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Eukaryotes generally have multiple isoforms of LC-FACS genes with multiple splice variants. For example, nine genes are found in Arabidopsis and six genes are expressed in mammalian cells. Drosophila melanogaster mutant bubblegum (BGM) have elevated levels of very-long-chain fatty acids (VLCFA) caused by a defective gene later named bubblegum. The human homolog (hsBG) of bubblegum has been characterized as a very long chain fatty acid CoA synthetase that functions specifically in the brain; hsBG may play a central role in brain VLCFA metabolism and myelinogenesis. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.
Pssm-ID: 341233 [Multi-domain] Cd Length: 452 Bit Score: 100.36 E-value: 3.09e-22
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 162 LTYSELLEKVCQLANYLKSLGVGKGDAVIIYLPMLLELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPKLVItcn 241
Cdd:cd05907 6 ITWAEFAEEVRALAKGLIALGVEPGDRVAILSRNRPEWTIADLAILAIGAVPVPIYPTSSAEQIAYILNDSEAKALF--- 82
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 242 avkrgvkpiflkdivdvalvesekngvsvglcltyenqsamkredtkwqperdvwwqdvvtkfptkcdVEwvDAEDPLFL 321
Cdd:cd05907 83 --------------------------------------------------------------------VE--DPDDLATI 92
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 322 LYTSGSTGKPKGVLHTSGGYMvYTATTFKYAFDYKPTDIYWCTADCGWITGHSYVTYGPLLNGATVLVFEGTPNYPDSGR 401
Cdd:cd05907 93 IYTSGTTGRPKGVMLSHRNIL-SNALALAERLPATEGDRHLSFLPLAHVFERRAGLYVPLLAGARIYFASSAETLLDDLS 171
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 402 cwdivdKYNVTIFYTAPTLVRSL-----MRDGTEY----VTRYSRKSLRVLGSVGEPINPSAWRwFYNIVGdsrCPISDT 472
Cdd:cd05907 172 ------EVRPTVFLAVPRVWEKVyaaikVKAVPGLkrklFDLAVGGRLRFAASGGAPLPAELLH-FFRALG---IPVYEG 241
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 473 WWQTETGGFMITPLPGAWpqKPGSATFPFFGVQpVIVDEKGqEIegECSGylcikkswPGAFRTLYGDHEryETTYFKPF 552
Cdd:cd05907 242 YGLTETSAVVTLNPPGDN--RIGTVGKPLPGVE-VRIADDG-EI--LVRG--------PNVMLGYYKNPE--ATAEALDA 305
|
410 420 430 440 450
....*....|....*....|....*....|....*....|....*....|....*...
gi 242061796 553 AGYYFTGDGCSRDKDGYHWLTGRVDDVI-NVSGHRIGTAEVESALVSHPQCAEAAVVG 609
Cdd:cd05907 306 DGWLHTGDLGEIDEDGFLHITGRKKDLIiTSGGKNISPEPIENALKASPLISQAVVIG 363
|
|
| PRK07514 |
PRK07514 |
malonyl-CoA synthase; Validated |
162-676 |
3.29e-22 |
|
malonyl-CoA synthase; Validated
Pssm-ID: 181011 [Multi-domain] Cd Length: 504 Bit Score: 100.72 E-value: 3.29e-22
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 162 LTYSELLEKVCQLANYLKSLGVGKGD-----------AVIIYLpmllelpiamlACARIGAVHSVVFAGFSADSLAQRIV 230
Cdd:PRK07514 29 YTYGDLDAASARLANLLVALGVKPGDrvavqvekspeALALYL-----------ATLRAGAVFLPLNTAYTLAELDYFIG 97
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 231 DCKPKLVITCNAVKRGVKPIflkdivdvalveSEKNGVSVGLCLTYENQSAMkredtkwqperdvwwQDVVTKFPTKCDV 310
Cdd:PRK07514 98 DAEPALVVCDPANFAWLSKI------------AAAAGAPHVETLDADGTGSL---------------LEAAAAAPDDFET 150
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 311 EWVDAEDPLFLLYTSGSTGKPKGVLHTSGGyMVYTATTFKYAFDYKPTD-------IYwctadcgwitgHSY----VTYG 379
Cdd:PRK07514 151 VPRGADDLAAILYTSGTTGRSKGAMLSHGN-LLSNALTLVDYWRFTPDDvlihalpIF-----------HTHglfvATNV 218
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 380 PLLNGATVL---------VFEGTPnypdsgRCwdivdkynvTIFYTAPTL-VRSLMRDG-TEYVTRYSRksLRVLGSVge 448
Cdd:PRK07514 219 ALLAGASMIflpkfdpdaVLALMP------RA---------TVMMGVPTFyTRLLQEPRlTREAAAHMR--LFISGSA-- 279
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 449 PINPSAWRWFYNIVGDsrcPISDTWWQTETGgfMIT--PLPGAwpQKPGSATFPFFGVQPVIVD-EKGQEIEGECSGYLC 525
Cdd:PRK07514 280 PLLAETHREFQERTGH---AILERYGMTETN--MNTsnPYDGE--RRAGTVGFPLPGVSLRVTDpETGAELPPGEIGMIE 352
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 526 IKKswPGAFRtlyGDHERYETTY--FKPfAGYYFTGDGCSRDKDGYHWLTGRVDDVINVSGHRIGTAEVESALVSHPQCA 603
Cdd:PRK07514 353 VKG--PNVFK---GYWRMPEKTAeeFRA-DGFFITGDLGKIDERGYVHIVGRGKDLIISGGYNVYPKEVEGEIDELPGVV 426
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 242061796 604 EAAVVGVEHEVKGQGIYAFVTLVDGVPYSEElrkSLIMTVRNQIGAFAAPDKIHWAPGLPKTRSGKIMRRILR 676
Cdd:PRK07514 427 ESAVIGVPHPDFGEGVTAVVVPKPGAALDEA---AILAALKGRLARFKQPKRVFFVDELPRNTMGKVQKNLLR 496
|
|
| A_NRPS_GliP_like |
cd17653 |
nonribosomal peptide synthase GliP-like; This family includes the adenylation (A) domain of ... |
157-677 |
6.40e-22 |
|
nonribosomal peptide synthase GliP-like; This family includes the adenylation (A) domain of nonribosomal peptide synthases (NRPS) gliotoxin biosynthesis protein P (GliP), thioclapurine biosynthesis protein P (tcpP) and Sirodesmin biosynthesis protein P (SirP). In the filamentous fungus Aspergillus fumigatus, NRPS GliP is involved in the biosynthesis of gliotoxin, which is initiated by the condensation of serine and phenylalanine. Studies show that GliP is not required for invasive aspergillosis, suggesting that the principal targets of gliotoxin are neutrophils or other phagocytes. SirP is a phytotoxin produced by the fungus Leptosphaeria maculans, which causes blackleg disease of canola (Brassica napus). In the fungus Claviceps purpurea, NRPS tcpP catalyzes condensation of tyrosine and glycine, part of biosynthesis of an unusual class of epipolythiodioxopiperazines (ETPs) that lacks the reactive thiol group for toxicity. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341308 [Multi-domain] Cd Length: 433 Bit Score: 99.31 E-value: 6.40e-22
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 157 SQDGKLTYSELLEKVCQLANYLKSLGVGKGDAVIIYLPMLLELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPKL 236
Cdd:cd17653 18 SLGGSLTYGELDAASNALANRLLQLGVVPGDVVPLLSDRSLEMLVAILAILKAGAAYVPLDAKLPSARIQAILRTSGATL 97
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 237 VITCNAvkrgvkpiflkdivdvalvesekngvsvglcltyenqsamkredtkwqperdvwwqdvvtkfptkcdvewvdAE 316
Cdd:cd17653 98 LLTTDS------------------------------------------------------------------------PD 105
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 317 DPLFLLYTSGSTGKPKGVLHTSGGYMVYTATT---------------FKYAFDYkptdiywCTAdcgwitghsyVTYGPL 381
Cdd:cd17653 106 DLAYIIFTSGSTGIPKGVMVPHRGVLNYVSQPparldvgpgsrvaqvLSIAFDA-------CIG----------EIFSTL 168
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 382 LNGATvLVFEGTPNYpdsgrcWDIVDKyNVTIFYTAPTLVRSLMRDgteyvtRYSRKSLRVLGsvGEPINPS---AW--- 455
Cdd:cd17653 169 CNGGT-LVLADPSDP------FAHVAR-TVDALMSTPSILSTLSPQ------DFPNLKTIFLG--GEAVPPSlldRWspg 232
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 456 RWFYNIVGDSRCPISDTWWQTETGGFMI--TPLPGAwpqkpgsatfpffGVqpVIVDEKGQEIEGECSGYLCIkkSWPGA 533
Cdd:cd17653 233 RRLYNAYGPTECTISSTMTELLPGQPVTigKPIPNS-------------TC--YILDADLQPVPEGVVGEICI--SGVQV 295
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 534 FRTLYGDHERyETTYFKPFAGY-----YFTGDGCSRDKDGYHWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAAVV 608
Cdd:cd17653 296 ARGYLGNPAL-TASKFVPDPFWpgsrmYRTGDYGRWTEDGGLEFLGREDNQVKVRGFRINLEEIEEVVLQSQPEVTQAAA 374
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 242061796 609 gvehEVKGQGIYAFVT--LVDGvpysEELRKSLimtvRNQIGAFAAPDKIHWAPGLPKTRSGKIMRRILRK 677
Cdd:cd17653 375 ----IVVNGRLVAFVTpeTVDV----DGLRSEL----AKHLPSYAVPDRIIALDSFPLTANGKVDRKALRE 433
|
|
| PRK12316 |
PRK12316 |
peptide synthase; Provisional |
159-683 |
7.85e-22 |
|
peptide synthase; Provisional
Pssm-ID: 237054 [Multi-domain] Cd Length: 5163 Bit Score: 101.57 E-value: 7.85e-22
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 159 DGKLTYSELLEKVCQLANYLKSLGVGKGDAVIIYLPMLLELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPKLVI 238
Cdd:PRK12316 3080 EQRLSYAELNRRANRLAHRLIERGVGPDVLVGVAVERSLEMVVGLLAILKAGGAYVPLDPEYPEERLAYMLEDSGAQLLL 3159
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 239 TCNAVKrgvkpIFLKDIVDVALVESEKNGVSvglcltYENQSAMkredtkwqperdvwwqdvvtkfptkcdvewVDAEDP 318
Cdd:PRK12316 3160 SQSHLR-----LPLAQGVQVLDLDRGDENYA------EANPAIR------------------------------TMPENL 3198
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 319 LFLLYTSGSTGKPKGVLHTSGGYMVYT-ATTFKYAFDYKPTDIYWCTADcgwITGHSYVTYGPLLNGATVLVfEGTPNYP 397
Cdd:PRK12316 3199 AYVIYTSGSTGKPKGVGIRHSALSNHLcWMQQAYGLGVGDRVLQFTTFS---FDVFVEELFWPLMSGARVVL-AGPEDWR 3274
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 398 DSGRCWDIVDKYNVTIfytaPTLVRSLMRDGTEYVTRYSRKSLRVLGSVGEPINPS-AWRWF-----YNIVGDSRCPISD 471
Cdd:PRK12316 3275 DPALLVELINSEGVDV----LHAYPSMLQAFLEEEDAHRCTSLKRIVCGGEALPADlQQQVFaglplYNLYGPTEATITV 3350
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 472 TWWQTETGGFMITPLPGAWPQKpgSATFPFFGVQPVIVDEKGQEI---EGECSGYLcikkSWPGAfrtlygDHERYETTY 548
Cdd:PRK12316 3351 THWQCVEEGKDAVPIGRPIANR--ACYILDGSLEPVPVGALGELYlggEGLARGYH----NRPGL------TAERFVPDP 3418
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 549 FKPFAGYYFTGDGCSRDKDGYHWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAAVVGVEhevkGQGIYAFVTLVDG 628
Cdd:PRK12316 3419 FVPGERLYRTGDLARYRADGVIEYIGRVDHQVKIRGFRIELGEIEARLLEHPWVREAVVLAVD----GRQLVAYVVPEDE 3494
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*
gi 242061796 629 VPyseELRKSLIMTVRNQIGAFAAPDKIHWAPGLPKTRSGKIMRRILRKIAARQL 683
Cdd:PRK12316 3495 AG---DLREALKAHLKASLPEYMVPAHLLFLERMPLTPNGKLDRKALPRPDAALL 3546
|
|
| PRK06060 |
PRK06060 |
p-hydroxybenzoic acid--AMP ligase FadD22; |
136-676 |
2.30e-21 |
|
p-hydroxybenzoic acid--AMP ligase FadD22;
Pssm-ID: 180374 [Multi-domain] Cd Length: 705 Bit Score: 98.95 E-value: 2.30e-21
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 136 DRNIESGNGDKIAMYwegnEPSQdgkLTYSELLEKVCQLANYLKSLGVGKGDAVIIYLPMLLELPIAMLACARIGAVHSV 215
Cdd:PRK06060 12 EQASEAGWYDRPAFY----AADV---VTHGQIHDGAARLGEVLRNRGLSSGDRVLLCLPDSPDLVQLLLACLARGVMAFL 84
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 216 VFAGFSADSLAQRIVDCKPKLVITCNAVKRGVKPiflKDIVDVALVESEKngvsvglcltyenqsamkredTKWQPErdv 295
Cdd:PRK06060 85 ANPELHRDDHALAARNTEPALVVTSDALRDRFQP---SRVAEAAELMSEA---------------------ARVAPG--- 137
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 296 wwqdvvtkfptkcDVEWVDAEDPLFLLYTSGSTGKPKGVLHTSGGYMVYTATTFKYAFDYKPTDIYWCTADCGWITGHSY 375
Cdd:PRK06060 138 -------------GYEPMGGDALAYATYTSGTTGPPKAAIHRHADPLTFVDAMCRKALRLTPEDTGLCSARMYFAYGLGN 204
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 376 VTYGPLLNGATVLVfEGTPNYPDSGRCwdIVDKYNVTIFYTAPTLVRSLMrdgtEYVTRYSRKSLRVLGSVGEPINPSAW 455
Cdd:PRK06060 205 SVWFPLATGGSAVI-NSAPVTPEAAAI--LSARFGPSVLYGVPNFFARVI----DSCSPDSFRSLRCVVSAGEALELGLA 277
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 456 RWFYNIVGDsrCPISDTWWQTETGGFMITPLPGAWpqKPGS--ATFPFFGVQPVIVD--EKGQEIEGEcsgyLCIKKswP 531
Cdd:PRK06060 278 ERLMEFFGG--IPILDGIGSTEVGQTFVSNRVDEW--RLGTlgRVLPPYEIRVVAPDgtTAGPGVEGD----LWVRG--P 347
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 532 GAFRTLYGDHERYETTyfkpfAGYYFTGDGCSRDKDGYHWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAAVVGVE 611
Cdd:PRK06060 348 AIAKGYWNRPDSPVAN-----EGWLDTRDRVCIDSDGWVTYRCRADDTEVIGGVNVDPREVERLIIEDEAVAEAAVVAVR 422
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 242061796 612 HEVKGQGIYAFVTLVDGVPYSEELRKSLIMTVRNQIGAFAAPDKIHWAPGLPKTRSGKIMRRILR 676
Cdd:PRK06060 423 ESTGASTLQAFLVATSGATIDGSVMRDLHRGLLNRLSAFKVPHRFAVVDRLPRTPNGKLVRGALR 487
|
|
| Firefly_Luc |
cd17642 |
insect luciferase, similar to plant 4-coumarate: CoA ligases; This family contains insect ... |
163-678 |
3.25e-21 |
|
insect luciferase, similar to plant 4-coumarate: CoA ligases; This family contains insect firefly luciferases that share significant sequence similarity to plant 4-coumarate:coenzyme A ligases, despite their functional diversity. Luciferase catalyzes the production of light in the presence of MgATP, molecular oxygen, and luciferin. In the first step, luciferin is activated by acylation of its carboxylate group with ATP, resulting in an enzyme-bound luciferyl adenylate. In the second step, luciferyl adenylate reacts with molecular oxygen, producing an enzyme-bound excited state product (Luc=O*) and releasing AMP. This excited-state product then decays to the ground state (Luc=O), emitting a quantum of visible light.
Pssm-ID: 341297 [Multi-domain] Cd Length: 532 Bit Score: 97.98 E-value: 3.25e-21
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 163 TYSELLEKVCQLANYLKSLGVGKGDAVIIYLPMLLELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPKLVItcnA 242
Cdd:cd17642 46 SYAEYLEMSVRLAEALKKYGLKQNDRIAVCSENSLQFFLPVIAGLFIGVGVAPTNDIYNERELDHSLNISKPTIVF---C 122
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 243 VKRGVKpiflkdivDVALVESEKNGVSVGLCLTYENQSAMKREDTKWQPERDVWWQDVVTKFPTKCDVEwvdaEDPLFLL 322
Cdd:cd17642 123 SKKGLQ--------KVLNVQKKLKIIKTIIILDSKEDYKGYQCLYTFITQNLPPGFNEYDFKPPSFDRD----EQVALIM 190
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 323 YTSGSTGKPKGVLHTSGGYMVytatTFKYAFDykPTDIYWCTADCGWIT----GHSY---VTYGPLLNGATVLVFegtPN 395
Cdd:cd17642 191 NSSGSTGLPKGVQLTHKNIVA----RFSHARD--PIFGNQIIPDTAILTvipfHHGFgmfTTLGYLICGFRVVLM---YK 261
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 396 YpDSGRCWDIVDKYNVTIFYTAPTLVRSLMRdgTEYVTRYSRKSLRVLGSVGEPINPSawrwfyniVGDS-----RCP-I 469
Cdd:cd17642 262 F-EEELFLRSLQDYKVQSALLVPTLFAFFAK--STLVDKYDLSNLHEIASGGAPLSKE--------VGEAvakrfKLPgI 330
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 470 SDTWWQTE-TGGFMITPlpgAWPQKPGSA--TFPFFGVQpVIVDEKGQEIEGECSGYLCIKKswPGAFRTLYGDHERYET 546
Cdd:cd17642 331 RQGYGLTEtTSAILITP---EGDDKPGAVgkVVPFFYAK-VVDLDTGKTLGPNERGELCVKG--PMIMKGYVNNPEATKA 404
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 547 TYFKPfaGYYFTGDGCSRDKDGYHWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAAVVGVEHEVKGQGIYAFVTLV 626
Cdd:cd17642 405 LIDKD--GWLHSGDIAYYDEDGHFFIVDRLKSLIKYKGYQVPPAELESILLQHPKIFDAGVAGIPDEDAGELPAAVVVLE 482
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*....
gi 242061796 627 DGVPYSEelrKSLIMTVRNQI-------GAFAAPDKIhwapglPKTRSGKIMRRILRKI 678
Cdd:cd17642 483 AGKTMTE---KEVMDYVASQVstakrlrGGVKFVDEV------PKGLTGKIDRRKIREI 532
|
|
| FACL_like_5 |
cd05924 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ... |
316-669 |
1.41e-20 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341248 [Multi-domain] Cd Length: 364 Bit Score: 93.99 E-value: 1.41e-20
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 316 EDPLFLLYTSGSTGKPKGVLHTSGGYMVYTATTFKYAFDyKPTDIYWCT------------ADCGWITGHSYVTYGPLLN 383
Cdd:cd05924 3 ADDLYILYTGGTTGMPKGVMWRQEDIFRMLMGGADFGTG-EFTPSEDAHkaaaaaagtvmfPAPPLMHGTGSWTAFGGLL 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 384 GATVLVFEGTPNYPDSgrCWDIVDKYNVTIF------YTAPtLVRSLMRDGTeyvtrYSRKSLRVLGSVGEPINPSAWRW 457
Cdd:cd05924 82 GGQTVVLPDDRFDPEE--VWRTIEKHKVTSMtivgdaMARP-LIDALRDAGP-----YDLSSLFAISSGGALLSPEVKQG 153
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 458 FYNIVGDSRcpISDTWWQTETGgFMITPLPGawPQKPGSATFPFFGVQPVIVDEKGQEIE--GECSGYLCIKKSWPGAFr 535
Cdd:cd05924 154 LLELVPNIT--LVDAFGSSETG-FTGSGHSA--GSGPETGPFTRANPDTVVLDDDGRVVPpgSGGVGWIARRGHIPLGY- 227
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 536 tlYGDHERYETTyFKPFAG--YYFTGDGCSRDKDGYHWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAAVVGVEHE 613
Cdd:cd05924 228 --YGDEAKTAET-FPEVDGvrYAVPGDRATVEADGTVTLLGRGSVCINTGGEKVFPEEVEEALKSHPAVYDVLVVGRPDE 304
|
330 340 350 360 370
....*....|....*....|....*....|....*....|....*....|....*.
gi 242061796 614 VKGQGIYAFVTLVDGVPYSEElrkSLIMTVRNQIGAFAAPDKIHWAPGLPKTRSGK 669
Cdd:cd05924 305 RWGQEVVAVVQLREGAGVDLE---ELREHCRTRIARYKLPKQVVFVDEIERSPAGK 357
|
|
| PRK08974 |
PRK08974 |
long-chain-fatty-acid--CoA ligase FadD; |
162-676 |
2.09e-20 |
|
long-chain-fatty-acid--CoA ligase FadD;
Pssm-ID: 236359 [Multi-domain] Cd Length: 560 Bit Score: 95.51 E-value: 2.09e-20
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 162 LTYSELLEKVCQLANYLKS-LGVGKGDAVIIYLPMLLELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPK-LVIT 239
Cdd:PRK08974 49 MTFRKLEERSRAFAAYLQNgLGLKKGDRVALMMPNLLQYPIALFGILRAGMIVVNVNPLYTPRELEHQLNDSGAKaIVIV 128
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 240 CNAVKRGVKPIFLKDIVDVALVE-----SEKNGVSVGLCLTYenqsaMKREDTKWQPERDVWWQDVVTKFP----TKCDV 310
Cdd:PRK08974 129 SNFAHTLEKVVFKTPVKHVILTRmgdqlSTAKGTLVNFVVKY-----IKRLVPKYHLPDAISFRSALHKGRrmqyVKPEL 203
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 311 EwvdAEDPLFLLYTSGSTGKPKGVLHTSGGyMVytATTFKyafdykptdiywctadCGWItghsyvtYGPLLNGATVLVF 390
Cdd:PRK08974 204 V---PEDLAFLQYTGGTTGVAKGAMLTHRN-ML--ANLEQ----------------AKAA-------YGPLLHPGKELVV 254
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 391 EGTPNY---PDSGRCW----------------DI------VDKYNVTIFYTAPTLVRSLMRDGTEYVTRYSRKSLRVLGs 445
Cdd:PRK08974 255 TALPLYhifALTVNCLlfielggqnllitnprDIpgfvkeLKKYPFTAITGVNTLFNALLNNEEFQELDFSSLKLSVGG- 333
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 446 vGEPINPS-AWRWfYNIVGdsrCPISDTWWQTETggfmiTPLPGAWP----QKPGSATFPFFGVQPVIVDEKGQEIEGEC 520
Cdd:PRK08974 334 -GMAVQQAvAERW-VKLTG---QYLLEGYGLTEC-----SPLVSVNPydldYYSGSIGLPVPSTEIKLVDDDGNEVPPGE 403
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 521 SGYLCIKKSwpgafRTLYGDHERYETTYFKPFAGYYFTGDGCSRDKDGYHWLTGRVDDVINVSGHRIGTAEVESALVSHP 600
Cdd:PRK08974 404 PGELWVKGP-----QVMLGYWQRPEATDEVIKDGWLATGDIAVMDEEGFLRIVDRKKDMILVSGFNVYPNEIEDVVMLHP 478
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 242061796 601 QCAEAAVVGVEHEVKGQGIYAFVTLVDGVPYSEELRKSlimtVRNQIGAFAAPDKIHWAPGLPKTRSGKIMRRILR 676
Cdd:PRK08974 479 KVLEVAAVGVPSEVSGEAVKIFVVKKDPSLTEEELITH----CRRHLTGYKVPKLVEFRDELPKSNVGKILRRELR 550
|
|
| PRK06018 |
PRK06018 |
putative acyl-CoA synthetase; Provisional |
302-677 |
2.70e-20 |
|
putative acyl-CoA synthetase; Provisional
Pssm-ID: 235673 [Multi-domain] Cd Length: 542 Bit Score: 95.20 E-value: 2.70e-20
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 302 TKFP-TKCDVEWVDAEDPLF------------LLYTSGSTGKPKGVLHTSGGYMVYT-ATTFKYAFDYKPTDIY------ 361
Cdd:PRK06018 150 TTLKnAVAYEEWIAEADGDFawktfdentaagMCYTSGTTGDPKGVLYSHRSNVLHAlMANNGDALGTSAADTMlpvvpl 229
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 362 -----WCTADCGWITGHSYVTYGPLLNGATVlvfegtpnypdsgrcWDIVDKYNVTIFYTAPTLVRSLMrdgtEYVTRyS 436
Cdd:PRK06018 230 fhansWGIAFSAPSMGTKLVMPGAKLDGASV---------------YELLDTEKVTFTAGVPTVWLMLL----QYMEK-E 289
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 437 RKSLRVLGSV---GEPINPSAWRWFYnivgDSRCPISDTWWQTETGGF-MITPLPGAWPQKPGSAT--------FPFFGV 504
Cdd:PRK06018 290 GLKLPHLKMVvcgGSAMPRSMIKAFE----DMGVEVRHAWGMTEMSPLgTLAALKPPFSKLPGDARldvlqkqgYPPFGV 365
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 505 QPVIVDEKGQEI--EGECSGYLciKKSWPGAFRTLYgdheRYETTYFKPfAGYYFTGDGCSRDKDGYHWLTGRVDDVINV 582
Cdd:PRK06018 366 EMKITDDAGKELpwDGKTFGRL--KVRGPAVAAAYY----RVDGEILDD-DGFFDTGDVATIDAYGYMRITDRSKDVIKS 438
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 583 SGHRIGTAEVESALVSHPQCAEAAVVGVEHEVKGQGIYAFVTLVDGV-PYSEELRKSLimtvRNQIGAFAAPDKIHWAPG 661
Cdd:PRK06018 439 GGEWISSIDLENLAVGHPKVAEAAVIGVYHPKWDERPLLIVQLKPGEtATREEILKYM----DGKIAKWWMPDDVAFVDA 514
|
410
....*....|....*.
gi 242061796 662 LPKTRSGKIMRRILRK 677
Cdd:PRK06018 515 IPHTATGKILKTALRE 530
|
|
| FAAL |
cd05931 |
Fatty acyl-AMP ligase (FAAL); FAAL belongs to the class I adenylate forming enzyme family and ... |
144-683 |
2.71e-20 |
|
Fatty acyl-AMP ligase (FAAL); FAAL belongs to the class I adenylate forming enzyme family and is homologous to fatty acyl-coenzyme A (CoA) ligases (FACLs). However, FAALs produce only the acyl adenylate and are unable to perform the thioester-forming reaction, while FACLs perform a two-step catalytic reaction; AMP ligation followed by CoA ligation using ATP and CoA as cofactors. FAALs have insertion motifs between the N-terminal and C-terminal subdomains that distinguish them from the FACLs. This insertion motif precludes the binding of CoA, thus preventing CoA ligation. It has been suggested that the acyl adenylates serve as substrates for multifunctional polyketide synthases to permit synthesis of complex lipids such as phthiocerol dimycocerosate, sulfolipids, mycolic acids, and mycobactin.
Pssm-ID: 341254 [Multi-domain] Cd Length: 547 Bit Score: 95.00 E-value: 2.71e-20
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 144 GDKIAMYWEGNEPSQDGKLTYSELLEKVCQLANYLKSLGvGKGDAVIIYLPMLLELPIAMLACARIGAVHSVVFA---GF 220
Cdd:cd05931 7 PDRPAYTFLDDEGGREETLTYAELDRRARAIAARLQAVG-KPGDRVLLLAPPGLDFVAAFLGCLYAGAIAVPLPPptpGR 85
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 221 SADSLAQRIVDCKPKLVITCNAVKRGVKPIFLKDIVDVALvesekngvsvglcltyenqsAMKREDTKWQPERDVWwqdv 300
Cdd:cd05931 86 HAERLAAILADAGPRVVLTTAAALAAVRAFAASRPAAGTP--------------------RLLVVDLLPDTSAADW---- 141
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 301 vtkfptkcDVEWVDAEDPLFLLYTSGSTGKPKGVLHTSGGYMVYTATTFKyAFDYKPTDIY--W--CTADCGWITGhsyv 376
Cdd:cd05931 142 --------PPPSPDPDDIAYLQYTSGSTGTPKGVVVTHRNLLANVRQIRR-AYGLDPGDVVvsWlpLYHDMGLIGG---- 208
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 377 TYGPLLNGATVLVFegTPNY----PdsGRcW-DIVDKYNVTIFYtAPT-----LVRSLMRDGTEYVtRYSrkSLRVLGSV 446
Cdd:cd05931 209 LLTPLYSGGPSVLM--SPAAflrrP--LR-WlRLISRYRATISA-APNfaydlCVRRVRDEDLEGL-DLS--SWRVALNG 279
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 447 GEPINPSAWRWFYN--------------------------IVGDSRCPISDTWWQTETGGfmitPLPGAWPQKPGSATF- 499
Cdd:cd05931 280 AEPVRPATLRRFAEafapfgfrpeafrpsyglaeatlfvsGGPPGTGPVVLRVDRDALAG----RAVAVAADDPAARELv 355
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 500 ----PFFGVQPVIVDEKGQE-----IEGE--------CSGYLCIKKSWPGAFRTLYGDHERyettyfkpfaGYYFTGD-G 561
Cdd:cd05931 356 scgrPLPDQEVRIVDPETGRelpdgEVGEiwvrgpsvASGYWGRPEATAETFGALAATDEG----------GWLRTGDlG 425
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 562 CSRdkDGYHWLTGRVDDVINVSGHRIGTAEVE-SALVSHPQ--CAEAAVVGVEHEVKGQGIyaFVTLVDGVPYSEE---L 635
Cdd:cd05931 426 FLH--DGELYITGRLKDLIIVRGRNHYPQDIEaTAEEAHPAlrPGCVAAFSVPDDGEERLV--VVAEVERGADPADlaaI 501
|
570 580 590 600 610
....*....|....*....|....*....|....*....|....*....|
gi 242061796 636 RKSLIMTVRNQIGafAAPDKIHWAP--GLPKTRSGKIMRRilrkiAARQL 683
Cdd:cd05931 502 AAAIRAAVAREHG--VAPADVVLVRpgSIPRTSSGKIQRR-----ACRAA 544
|
|
| A_NRPS_SidN3_like |
cd05918 |
The adenylation (A) domain of siderophore-synthesizing nonribosomal peptide synthetases (NRPS); ... |
157-681 |
2.97e-20 |
|
The adenylation (A) domain of siderophore-synthesizing nonribosomal peptide synthetases (NRPS); The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. This family of siderophore-synthesizing NRPS includes the third adenylation domain of SidN from the endophytic fungus Neotyphodium lolii, ferrichrome siderophore synthetase, HC-toxin synthetase, and enniatin synthase. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341242 [Multi-domain] Cd Length: 481 Bit Score: 94.53 E-value: 2.97e-20
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 157 SQDGKLTYSELLEKVCQLANYLKSLGVGKGDAVIIYLPMLLELPIAMLACARIGAvhsvVFAGFSADSLAQRIV----DC 232
Cdd:cd05918 20 AWDGSLTYAELDRLSSRLAHHLRSLGVGPGVFVPLCFEKSKWAVVAMLAVLKAGG----AFVPLDPSHPLQRLQeilqDT 95
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 233 KPKLVITCnavkrgvkpiflkdivdvalvesekngvsvglcltyenqsamkredtkwqperdvwwqdvvtkfptkcdvew 312
Cdd:cd05918 96 GAKVVLTS------------------------------------------------------------------------ 103
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 313 vDAEDPLFLLYTSGSTGKPKGVL--HTSggyMVYTATTFKYAFDYKPTDIYWCTAdcgwitghSYV-------TYGPLLN 383
Cdd:cd05918 104 -SPSDAAYVIFTSGSTGKPKGVVieHRA---LSTSALAHGRALGLTSESRVLQFA--------SYTfdvsileIFTTLAA 171
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 384 GATVLVfegtPnyPDSGRCWDIVD---KYNVT-IFYTaPTLVRSLMRDGTEyvtrysrkSLRVLGSVGEPINPSawrwfy 459
Cdd:cd05918 172 GGCLCI----P--SEEDRLNDLAGfinRLRVTwAFLT-PSVARLLDPEDVP--------SLRTLVLGGEALTQS------ 230
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 460 nivgdsrcpISDTWWQ----------TETGGFMITPLPGAwPQKPGSATFPfFGVQPVIVDEKGQE------------IE 517
Cdd:cd05918 231 ---------DVDTWADrvrlinaygpAECTIAATVSPVVP-STDPRNIGRP-LGATCWVVDPDNHDrlvpigavgellIE 299
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 518 GE--CSGYL---------------CIKKSWPGAFRTLygdheryettyfkpfagyYFTGDGCSRDKDG---YHwltGRVD 577
Cdd:cd05918 300 GPilARGYLndpektaaafiedpaWLKQEGSGRGRRL------------------YRTGDLVRYNPDGsleYV---GRKD 358
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 578 DVINVSGHRIGTAEVESALVSHPQCAEAAVVGVEHEVKGQG---IYAFVTLvDGVPYSEELRKSLIMTVRNQigAFAAPD 654
Cdd:cd05918 359 TQVKIRGQRVELGEIEHHLRQSLPGAKEVVVEVVKPKDGSSspqLVAFVVL-DGSSSGSGDGDSLFLEPSDE--FRALVA 435
|
570 580 590 600
....*....|....*....|....*....|....*....|....
gi 242061796 655 KI---------------HWAP--GLPKTRSGKIMRRILRKIAAR 681
Cdd:cd05918 436 ELrsklrqrlpsymvpsVFLPlsHLPLTASGKIDRRALRELAES 479
|
|
| ACLS-CaiC |
cd17637 |
acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II; This family contains fatty acid CoA ... |
317-672 |
4.42e-20 |
|
acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II; This family contains fatty acid CoA ligases, including acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II, most of which are yet to be characterized, but may be similar to Carnitine-CoA ligase (CaiC) which catalyzes the transfer of CoA to carnitine. Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341292 [Multi-domain] Cd Length: 333 Bit Score: 91.95 E-value: 4.42e-20
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 317 DPLFLLYTSGSTGKPKGVLHTSGGyMVYTATTFKYAFDYKPTDIYWCTADCGWITGHSYVTYGPLLNGATVLV--Fegtp 394
Cdd:cd17637 1 DPFVIIHTAAVAGRPRGAVLSHGN-LIAANLQLIHAMGLTEADVYLNMLPLFHIAGLNLALATFHAGGANVVMekF---- 75
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 395 nypDSGRCWDIVDKYNVTIFYTAPTLVRSLMRDGTEyvTRYSRKSLRVLGSVGEPINPSAWRWFYNivgdsrcpisDTWW 474
Cdd:cd17637 76 ---DPAEALELIEEEKVTLMGSFPPILSNLLDAAEK--SGVDLSSLRHVLGLDAPETIQRFEETTG----------ATFW 140
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 475 ----QTETGGFmITPLPGAwpQKPGSATFPFFGVQPVIVDEKGQEIEGECSGYLCIKKswPGAFRTLYGDHERYETTYFK 550
Cdd:cd17637 141 slygQTETSGL-VTLSPYR--ERPGSAGRPGPLVRVRIVDDNDRPVPAGETGEIVVRG--PLVFQGYWNLPELTAYTFRN 215
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 551 pfaGYYFTGDGCSRDKDGYHWLTGRV--DDVINVSGHRIGTAEVESALVSHPQCAEAAVVGVEHEVKGQGIYAFVTLVDG 628
Cdd:cd17637 216 ---GWHHTGDLGRFDEDGYLWYAGRKpeKELIKPGGENVYPAEVEKVILEHPAIAEVCVIGVPDPKWGEGIKAVCVLKPG 292
|
330 340 350 360
....*....|....*....|....*....|....*....|....
gi 242061796 629 VPYSEElrkSLIMTVRNQIGAFAAPDKIHWAPGLPKTRSGKIMR 672
Cdd:cd17637 293 ATLTAD---ELIEFVGSRIARYKKPRYVVFVEALPKTADGSIDR 333
|
|
| ACAS_N |
pfam16177 |
Acetyl-coenzyme A synthetase N-terminus; This domain is found at the N-terminus of many ... |
73-134 |
4.49e-20 |
|
Acetyl-coenzyme A synthetase N-terminus; This domain is found at the N-terminus of many acetyl-coenzyme A synthetase enzymes.
Pssm-ID: 465043 [Multi-domain] Cd Length: 55 Bit Score: 84.06 E-value: 4.49e-20
10 20 30 40 50 60
....*....|....*....|....*....|....*....|....*....|....*....|..
gi 242061796 73 YREMYERSIKNPAGFWSEIAETFYWKEKWnpaevcSENLDVTKGPvQINWFKGGKTNICYNA 134
Cdd:pfam16177 1 YEALYRRSIEDPEGFWGEVAKELDWFKPF------DKVLDGSNGP-FAKWFVGGKLNVCYNC 55
|
|
| PLN02860 |
PLN02860 |
o-succinylbenzoate-CoA ligase |
155-677 |
4.63e-20 |
|
o-succinylbenzoate-CoA ligase
Pssm-ID: 215464 [Multi-domain] Cd Length: 563 Bit Score: 94.48 E-value: 4.63e-20
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 155 EPSQDGKLTYSELLEKVCQLANYLKSLGVGKGDAVII-------YLPMllelpiaMLACARIGAVHSVVFAGFSADSLAQ 227
Cdd:PLN02860 26 TISGNRRRTGHEFVDGVLSLAAGLLRLGLRNGDVVAIaalnsdlYLEW-------LLAVACAGGIVAPLNYRWSFEEAKS 98
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 228 RIVDCKPKLVITCNAVKRGVKPIFLKDIVDV---ALVESEKNGVSVGLcltyenqSAMKREDTKWQPerdvwwqdvvTKF 304
Cdd:PLN02860 99 AMLLVRPVMLVTDETCSSWYEELQNDRLPSLmwqVFLESPSSSVFIFL-------NSFLTTEMLKQR----------ALG 161
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 305 PTKCDVEWVdAEDPLFLLYTSGSTGKPKGVL--HTSggymVYTATTFKYAF-DYKPTDIYWCTADCGWITGHSYVTYGPL 381
Cdd:PLN02860 162 TTELDYAWA-PDDAVLICFTSGTTGRPKGVTisHSA----LIVQSLAKIAIvGYGEDDVYLHTAPLCHIGGLSSALAMLM 236
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 382 LNGATVLVfegtPNYpDSGRCWDIVDKYNVTIFYTAPTLVRSLMRDGTEYVTRYSRKSLRVL----GSVGEPINPSAWRW 457
Cdd:PLN02860 237 VGACHVLL----PKF-DAKAALQAIKQHNVTSMITVPAMMADLISLTRKSMTWKVFPSVRKIlnggGSLSSRLLPDAKKL 311
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 458 FynivgdSRCPISDTWWQTETGG---FMITPLPGAWPQKPGSATFPFFGVQPVivdekgQEIEGECSG----YLCIKKSW 530
Cdd:PLN02860 312 F------PNAKLFSAYGMTEACSsltFMTLHDPTLESPKQTLQTVNQTKSSSV------HQPQGVCVGkpapHVELKIGL 379
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 531 PGAFRT----LYGDH--ERY-----ETTYFKPFAGYYFTGDGCSRDKDGYHWLTGRVDDVINVSGHRIGTAEVESALVSH 599
Cdd:PLN02860 380 DESSRVgrilTRGPHvmLGYwgqnsETASVLSNDGWLDTGDIGWIDKAGNLWLIGRSNDRIKTGGENVYPEEVEAVLSQH 459
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 600 PQCAEAAVVGVEHEVKGQGIYAFVTLVDGVPYS----EELRKSLIMT-------VRNQ-IGAFAAPDKI-HWAPGLPKTR 666
Cdd:PLN02860 460 PGVASVVVVGVPDSRLTEMVVACVRLRDGWIWSdnekENAKKNLTLSsetlrhhCREKnLSRFKIPKLFvQWRKPFPLTT 539
|
570
....*....|.
gi 242061796 667 SGKIMRRILRK 677
Cdd:PLN02860 540 TGKIRRDEVRR 550
|
|
| PLN03102 |
PLN03102 |
acyl-activating enzyme; Provisional |
161-679 |
5.15e-20 |
|
acyl-activating enzyme; Provisional
Pssm-ID: 215576 [Multi-domain] Cd Length: 579 Bit Score: 94.32 E-value: 5.15e-20
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 161 KLTYSELLEKVCQLANYLKSLGVGKGDAVIIYLPMLLELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPKLVItc 240
Cdd:PLN03102 39 RFTWPQTYDRCCRLAASLISLNITKNDVVSVLAPNTPAMYEMHFAVPMAGAVLNPINTRLDATSIAAILRHAKPKILF-- 116
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 241 naVKRGVKPIfLKDIVdvALVESEKNGVSVGLCLTYENQSamkredTKWQPERDVWWQDVVTKF-PTKCDVEWV----DA 315
Cdd:PLN03102 117 --VDRSFEPL-AREVL--HLLSSEDSNLNLPVIFIHEIDF------PKRPSSEELDYECLIQRGePTPSLVARMfriqDE 185
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 316 EDPLFLLYTSGSTGKPKGVLHT-SGGYMVYTATTFKYAFDYKPT-----DIYWCTadcGWItghsyVTYGPLLNGATVLV 389
Cdd:PLN03102 186 HDPISLNYTSGTTADPKGVVIShRGAYLSTLSAIIGWEMGTCPVylwtlPMFHCN---GWT-----FTWGTAARGGTSVC 257
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 390 FEgtpnYPDSGRCWDIVDKYNVTIFYTAPTLVRSLMRDGTeyvTRYSRKSLRVLGSVGEPINPSAwrwFYNIVGDSRCPI 469
Cdd:PLN03102 258 MR----HVTAPEIYKNIEMHNVTHMCCVPTVFNILLKGNS---LDLSPRSGPVHVLTGGSPPPAA---LVKKVQRLGFQV 327
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 470 SDTWWQTE-TGGFMITPLPGAWPQKPGSATFPFFGVQPVI------VDEKGQEI------EGECSGYLCIKKSwpgAFRT 536
Cdd:PLN03102 328 MHAYGLTEaTGPVLFCEWQDEWNRLPENQQMELKARQGVSilgladVDVKNKETqesvprDGKTMGEIVIKGS---SIMK 404
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 537 LYGDHERYETTYFKpfAGYYFTGDGCSRDKDGYHWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAAVVGVEHEVKG 616
Cdd:PLN03102 405 GYLKNPKATSEAFK--HGWLNTGDVGVIHPDGHVEIKDRSKDIIISGGENISSVEVENVLYKYPKVLETAVVAMPHPTWG 482
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 617 QGIYAFVTLVDGVPYSEEL-------RKSLIMTVRNQIGAFAAPDKIHWAPGLPKTRSGKIMRRILRKIA 679
Cdd:PLN03102 483 ETPCAFVVLEKGETTKEDRvdklvtrERDLIEYCRENLPHFMCPRKVVFLQELPKNGNGKILKPKLRDIA 552
|
|
| PRK08279 |
PRK08279 |
long-chain-acyl-CoA synthetase; Validated |
143-611 |
8.02e-20 |
|
long-chain-acyl-CoA synthetase; Validated
Pssm-ID: 236217 [Multi-domain] Cd Length: 600 Bit Score: 93.79 E-value: 8.02e-20
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 143 NGDKIAMYWEgnepsqDGKLTYSELLEKVCQLANYLKSLGVGKGDAVIIYLPMLLELPIAMLACARIGAVHSVVFAGFSA 222
Cdd:PRK08279 50 HPDRPALLFE------DQSISYAELNARANRYAHWAAARGVGKGDVVALLMENRPEYLAAWLGLAKLGAVVALLNTQQRG 123
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 223 DSLAQRIVDCKPKLVITCNAVkrgvkpiflkdivdVALVESEKNGVSVGLCLTYENQsamkreDTKWQPERDVWWQDVVT 302
Cdd:PRK08279 124 AVLAHSLNLVDAKHLIVGEEL--------------VEAFEEARADLARPPRLWVAGG------DTLDDPEGYEDLAAAAA 183
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 303 KFPTKCDVEW--VDAEDPLFLLYTSGSTGKPKGVLHTSGGYMVYTAtTFKYAFDYKPTDIYWCTADCGWITGHSYVTYGP 380
Cdd:PRK08279 184 GAPTTNPASRsgVTAKDTAFYIYTSGTTGLPKAAVMSHMRWLKAMG-GFGGLLRLTPDDVLYCCLPLYHNTGGTVAWSSV 262
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 381 LLNGATVLV---FEGTpnypdsgRCWDIVDKYNVTIFYTAPTLVRSLMRDGTEYVTRysRKSLRVLgsVGEPINPSAWRW 457
Cdd:PRK08279 263 LAAGATLALrrkFSAS-------RFWDDVRRYRATAFQYIGELCRYLLNQPPKPTDR--DHRLRLM--IGNGLRPDIWDE 331
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 458 FYNIVGDSRcpISDTWWQTEtGGFMITPLPGawpqKPGSATF-PFFGVQPV-IV-----------DEKGQEIE---GEcS 521
Cdd:PRK08279 332 FQQRFGIPR--ILEFYAASE-GNVGFINVFN----FDGTVGRvPLWLAHPYaIVkydvdtgepvrDADGRCIKvkpGE-V 403
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 522 GyLCIKKSWPGAFRTLYGDHERYE----TTYFKPFAGYYFTGDGCSRDKDGYHWLTGRVDDVI-----NVSghrigTAEV 592
Cdd:PRK08279 404 G-LLIGRITDRGPFDGYTDPEASEkkilRDVFKKGDAWFNTGDLMRDDGFGHAQFVDRLGDTFrwkgeNVA-----TTEV 477
|
490
....*....|....*....
gi 242061796 593 ESALVSHPQCAEAAVVGVE 611
Cdd:PRK08279 478 ENALSGFPGVEEAVVYGVE 496
|
|
| entE |
PRK10946 |
(2,3-dihydroxybenzoyl)adenylate synthase; |
161-683 |
1.29e-19 |
|
(2,3-dihydroxybenzoyl)adenylate synthase;
Pssm-ID: 236803 [Multi-domain] Cd Length: 536 Bit Score: 92.75 E-value: 1.29e-19
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 161 KLTYSELLEKVCQLANYLKSLGVGKGDAVIIYLPMLLELPIAMLACARIGAVhsVVFAGFSADSL-----AQRIvdcKPK 235
Cdd:PRK10946 48 QFSYRELNQASDNLACSLRRQGIKPGDTALVQLGNVAEFYITFFALLKLGVA--PVNALFSHQRSelnayASQI---EPA 122
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 236 LVITCNAvkrgvKPIFLKDIVDVALVEsEKNGVSVGLcltyenqsaMKREDTkwQPERDVWWQDVVTKF---PTKCDvew 312
Cdd:PRK10946 123 LLIADRQ-----HALFSDDDFLNTLVA-EHSSLRVVL---------LLNDDG--EHSLDDAINHPAEDFtatPSPAD--- 182
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 313 vdaEDPLFLLyTSGSTGKPKGVLHTSGGYmvytattfkyafDY---KPTDIYWCTAD----CGWITGHSYVTYGP----- 380
Cdd:PRK10946 183 ---EVAFFQL-SGGSTGTPKLIPRTHNDY------------YYsvrRSVEICGFTPQtrylCALPAAHNYPMSSPgalgv 246
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 381 -LLNGATVLVfegtPNyPDSGRCWDIVDKYNVTIFYTAPTLVRSLMRDGTEYVTRYSRKSLRVLGSVGEPINPSAWRWFY 459
Cdd:PRK10946 247 fLAGGTVVLA----PD-PSATLCFPLIEKHQVNVTALVPPAVSLWLQAIAEGGSRAQLASLKLLQVGGARLSETLARRIP 321
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 460 NIVGdsrCPIS----------------DTWWQT-ETGGFMITPLPGAWpqkpgsatfpffgvqpvIVDEKGQEI-EGEcS 521
Cdd:PRK10946 322 AELG---CQLQqvfgmaeglvnytrldDSDERIfTTQGRPMSPDDEVW-----------------VADADGNPLpQGE-V 380
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 522 GYLCIKKswPGAFRTLYGDHErYETTYFKPfAGYYFTGDGCSRDKDGYHWLTGRVDDVINVSGHRIGTAEVESALVSHPQ 601
Cdd:PRK10946 381 GRLMTRG--PYTFRGYYKSPQ-HNASAFDA-NGFYCSGDLVSIDPDGYITVVGREKDQINRGGEKIAAEEIENLLLRHPA 456
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 602 CAEAAVVGVEHEVKGQGIYAFVTLVDGVPySEELRKSLimtvRNQ-IGAFAAPDKIHWAPGLPKTRSGKIMRRILRKIAA 680
Cdd:PRK10946 457 VIHAALVSMEDELMGEKSCAFLVVKEPLK-AVQLRRFL----REQgIAEFKLPDRVECVDSLPLTAVGKVDKKQLRQWLA 531
|
...
gi 242061796 681 RQL 683
Cdd:PRK10946 532 SRA 534
|
|
| PLN02330 |
PLN02330 |
4-coumarate--CoA ligase-like 1 |
160-677 |
1.64e-19 |
|
4-coumarate--CoA ligase-like 1
Pssm-ID: 215189 [Multi-domain] Cd Length: 546 Bit Score: 92.73 E-value: 1.64e-19
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 160 GK-LTYSELLEKVCQLANYLKSLGVGKGDAVIIYLPMLLELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPKLVI 238
Cdd:PLN02330 53 GKaVTYGEVVRDTRRFAKALRSLGLRKGQVVVVVLPNVAEYGIVALGIMAAGGVFSGANPTALESEIKKQAEAAGAKLIV 132
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 239 TCNAVKRGVKPIFLKDIVdvaLVESEkngvsvglcltyeNQSAMKredtkwqperdvwWQDVVT---KFPTKCDVEWVDA 315
Cdd:PLN02330 133 TNDTNYGKVKGLGLPVIV---LGEEK-------------IEGAVN-------------WKELLEaadRAGDTSDNEEILQ 183
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 316 EDPLFLLYTSGSTGKPKGVLHTSGGYMVYTATTFkyaFDYKPTDIYWCTAdCGWIT-----GHSYVTYGPLLNGATVLVf 390
Cdd:PLN02330 184 TDLCALPFSSGTTGISKGVMLTHRNLVANLCSSL---FSVGPEMIGQVVT-LGLIPffhiyGITGICCATLRNKGKVVV- 258
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 391 egtpnypdsgrcwdiVDKYNVTIFYTA------------PTLVRSLMRDGTEYVTRYSRKSLRVLGSVGEPINPSAWRWF 458
Cdd:PLN02330 259 ---------------MSRFELRTFLNAlitqevsfapivPPIILNLVKNPIVEEFDLSKLKLQAIMTAAAPLAPELLTAF 323
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 459 YNIVGDSRcpISDTWWQTETGGFMIT---PLPGAWPQKPGSATFPFFGVQPVIVD-EKGQEIEGECSGYLCIKKSWpgAF 534
Cdd:PLN02330 324 EAKFPGVQ--VQEAYGLTEHSCITLThgdPEKGHGIAKKNSVGFILPNLEVKFIDpDTGRSLPKNTPGELCVRSQC--VM 399
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 535 RTLYGDHERYETTYFKpfAGYYFTGDGCSRDKDGYHWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAAVVGVEHEV 614
Cdd:PLN02330 400 QGYYNNKEETDRTIDE--DGWLHTGDIGYIDDDGDIFIVDRIKELIKYKGFQVAPAELEAILLTHPSVEDAAVVPLPDEE 477
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|...
gi 242061796 615 KGQGIYAFVTLVDGVPYSEElrkSLIMTVRNQIGAFAAPDKIHWAPGLPKTRSGKIMRRILRK 677
Cdd:PLN02330 478 AGEIPAACVVINPKAKESEE---DILNFVAANVAHYKKVRVVQFVDSIPKSLSGKIMRRLLKE 537
|
|
| FADD10 |
cd17635 |
adenylate forming domain, fatty acid CoA ligase (FadD10); This family contains long chain ... |
316-672 |
1.82e-19 |
|
adenylate forming domain, fatty acid CoA ligase (FadD10); This family contains long chain fatty acid CoA ligases, including FadD10 which is involved in the synthesis of a virulence-related lipopeptide. FadD10 is a fatty acyl-AMP ligase (FAAL) that transfers fatty acids to an acyl carrier protein. Structures of FadD10 in apo- and complexed form with dodecanoyl-AMP, show a novel open conformation, facilitated by its unique inter-domain and intermolecular interactions, which is critical for the enzyme to carry out the acyl transfer onto the acyl carrier protein (Rv0100) rather than coenzyme A.
Pssm-ID: 341290 [Multi-domain] Cd Length: 340 Bit Score: 90.40 E-value: 1.82e-19
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 316 EDPLFLLYTSGSTGKPKGVLHTSGGYMVYTATTFKYAFDYKPTDIYWCTADCGWITGHSYVTYGPLLNGATVLVFEGTpn 395
Cdd:cd17635 1 EDPLAVIFTSGTTGEPKAVLLANKTFFAVPDILQKEGLNWVVGDVTYLPLPATHIGGLWWILTCLIHGGLCVTGGENT-- 78
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 396 ypDSGRCWDIVDKYNVTIFYTAPTL---VRSLMRDGTEYVtrysrKSLRVLGSVGE-PINpsAWRWFYNIVGDSRcpISD 471
Cdd:cd17635 79 --TYKSLFKILTTNAVTTTCLVPTLlskLVSELKSANATV-----PSLRLIGYGGSrAIA--ADVRFIEATGLTN--TAQ 147
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 472 TWWQTETGGFMITPLpGAWPQKPGSATFPFFGVQPVIVDEKGQEIEGECSGYLCIKKSWpgafrTLYGDHERYETTYFKP 551
Cdd:cd17635 148 VYGLSETGTALCLPT-DDDSIEINAVGRPYPGVDVYLAATDGIAGPSASFGTIWIKSPA-----NMLGYWNNPERTAEVL 221
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 552 FAGYYFTGDGCSRDKDGYHWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAAVVGVEHEVKGQGIYAFVTLvdgvpy 631
Cdd:cd17635 222 IDGWVNTGDLGERREDGFLFITGRSSESINCGGVKIAPDEVERIAEGVSGVQECACYEISDEEFGELVGLAVVA------ 295
|
330 340 350 360
....*....|....*....|....*....|....*....|....*
gi 242061796 632 SEELRKS----LIMTVRNQIGAFAAPDKIHWAPGLPKTRSGKIMR 672
Cdd:cd17635 296 SAELDENairaLKHTIRRELEPYARPSTIVIVTDIPRTQSGKVKR 340
|
|
| PRK07867 |
PRK07867 |
acyl-CoA synthetase; Validated |
313-684 |
4.54e-19 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236120 [Multi-domain] Cd Length: 529 Bit Score: 91.28 E-value: 4.54e-19
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 313 VDAEDPLFLLYTSGSTGKPKGVLHTSGgYMVYTATTFKYAFDYKPTDIYWCTAD--------CGWITGhsyvtygpLLNG 384
Cdd:PRK07867 149 ADPDDLFMLIFTSGTSGDPKAVRCTHR-KVASAGVMLAQRFGLGPDDVCYVSMPlfhsnavmAGWAVA--------LAAG 219
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 385 ATVLV---FEGTPNYPDsgrcwdiVDKYNVTIF--------YTAPTLVRSLMRDGTeyvtrysrksLRVL-GSVGEPINP 452
Cdd:PRK07867 220 ASIALrrkFSASGFLPD-------VRRYGATYAnyvgkplsYVLATPERPDDADNP----------LRIVyGNEGAPGDI 282
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 453 SAWRWFYNivgdsrCPISDTWWQTEtGGFMITPLPGAwpqKPGSATFPFFGVQ-----------PVIVDEKGQEIEGECS 521
Cdd:PRK07867 283 ARFARRFG------CVVVDGFGSTE-GGVAITRTPDT---PPGALGPLPPGVAivdpdtgtecpPAEDADGRLLNADEAI 352
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 522 GYLcIKKSWPGAFRTLYGDHEryeTTYFKPFAGYYFTGDGCSRDKDGYHWLTGRVDDVINVSGHRIGTAEVESALVSHPQ 601
Cdd:PRK07867 353 GEL-VNTAGPGGFEGYYNDPE---ADAERMRGGVYWSGDLAYRDADGYAYFAGRLGDWMRVDGENLGTAPIERILLRYPD 428
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 602 CAEAAVVGVEHEVKGQGIYAFVTLVDGVPY-SEELRKSLimTVRNQIGAFAAPDKIHWAPGLPKTRSGKIMRRILRKIAA 680
Cdd:PRK07867 429 ATEVAVYAVPDPVVGDQVMAALVLAPGAKFdPDAFAEFL--AAQPDLGPKQWPSYVRVCAELPRTATFKVLKRQLSAEGV 506
|
....
gi 242061796 681 RQLD 684
Cdd:PRK07867 507 DCAD 510
|
|
| PRK12467 |
PRK12467 |
peptide synthase; Provisional |
161-683 |
6.49e-19 |
|
peptide synthase; Provisional
Pssm-ID: 237108 [Multi-domain] Cd Length: 3956 Bit Score: 92.15 E-value: 6.49e-19
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 161 KLTYSELLEKVCQLANYLKSLGVGKGDAVIIYLPMLLELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPKLVITC 240
Cdd:PRK12467 1599 ELTYGELNRRANRLAHRLIALGVGPEVLVGIAVERSLEMVVGLLAILKAGGAYVPLDPEYPRERLAYMIEDSGIELLLTQ 1678
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 241 NAVKR------GVKPIFLKDIVDVALVESEKNGVSVglcltyenqsamkredtkwqperdvwwqdvvtkfptkcdvewVD 314
Cdd:PRK12467 1679 SHLQArlplpdGLRSLVLDQEDDWLEGYSDSNPAVN------------------------------------------LA 1716
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 315 AEDPLFLLYTSGSTGKPKGVLHTSGGyMVYTATTFKYAFDYkptdiywCTADCgWITGHSYV-------TYGPLLNGAT- 386
Cdd:PRK12467 1717 PQNLAYVIYTSGSTGRPKGAGNRHGA-LVNRLCATQEAYQL-------SAADV-VLQFTSFAfdvsvweLFWPLINGARl 1787
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 387 VLVFEGTPNYPdsGRCWDIVDKYNVTIFYTAPTLVRSLMrdgtEYVTRYSR-KSLRVLGSVGEPINPSAWR-WF------ 458
Cdd:PRK12467 1788 VIAPPGAHRDP--EQLIQLIERQQVTTLHFVPSMLQQLL----QMDEQVEHpLSLRRVVCGGEALEVEALRpWLerlpdt 1861
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 459 --YNIVGDSRCPISDTWWqtetggfmitPLPGAWPQkpGSATFPFfgvqpvivdekGQEIeGECSGYL-----------C 525
Cdd:PRK12467 1862 glFNLYGPTETAVDVTHW----------TCRRKDLE--GRDSVPI-----------GQPI-ANLSTYIldaslnpvpigV 1917
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 526 IKKSWPGAFRTLYGDHERYETTYFK----PFAGY----YFTGDGCSRDKDGYHWLTGRVDDVINVSGHRIGTAEVESALV 597
Cdd:PRK12467 1918 AGELYLGGVGLARGYLNRPALTAERfvadPFGTVgsrlYRTGDLARYRADGVIEYLGRIDHQVKIRGFRIELGEIEARLR 1997
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 598 SHPQCAEAAVVGVEHEvKGQGIYAFVTLVD-GVPYSEELRKSLIMTVRNQIGAfAAPDKI---HWA--PGLPKTRSGKIM 671
Cdd:PRK12467 1998 EQGGVREAVVIAQDGA-NGKQLVAYVVPTDpGLVDDDEAQVALRAILKNHLKA-SLPEYMvpaHLVflARMPLTPNGKLD 2075
|
570
....*....|..
gi 242061796 672 RRILRKIAARQL 683
Cdd:PRK12467 2076 RKALPAPDASEL 2087
|
|
| LC_FACS_bac |
cd05932 |
Bacterial long-chain fatty acid CoA synthetase (LC-FACS), including Marinobacter ... |
162-609 |
6.64e-19 |
|
Bacterial long-chain fatty acid CoA synthetase (LC-FACS), including Marinobacter hydrocarbonoclasticus isoprenoid Coenzyme A synthetase; The members of this family are bacterial long-chain fatty acid CoA synthetase. Marinobacter hydrocarbonoclasticus isoprenoid Coenzyme A synthetase in this family is involved in the synthesis of isoprenoid wax ester storage compounds when grown on phytol as the sole carbon source. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.
Pssm-ID: 341255 [Multi-domain] Cd Length: 508 Bit Score: 90.61 E-value: 6.64e-19
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 162 LTYSELLEKVCQLANYLKSLGVGKGDAVIIYLPMLLELPIAMLACARIGAVHSVVFAGFSADSLAQrivdckpklVITcn 241
Cdd:cd05932 7 FTWGEVADKARRLAAALRALGLEPGSKIALISKNCAEWFITDLAIWMAGHISVPLYPTLNPDTIRY---------VLE-- 75
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 242 avKRGVKPIFLKDIVDValvESEKNGV--SVGLCLTYEnQSAMKREDTkwqperdvwWQDVVTKFPTKCDVEWVDAEDPL 319
Cdd:cd05932 76 --HSESKALFVGKLDDW---KAMAPGVpeGLISISLPP-PSAANCQYQ---------WDDLIAQHPPLEERPTRFPEQLA 140
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 320 FLLYTSGSTGKPKGVLHTSGGYmVYTATTFKYAFDYKPTDIYWCTADCGWITGHSYVTYGPLLNGATVLVFEGTPNYPDS 399
Cdd:cd05932 141 TLIYTSGTTGQPKGVMLTFGSF-AWAAQAGIEHIGTEENDRMLSYLPLAHVTERVFVEGGSLYGGVLVAFAESLDTFVED 219
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 400 grcwdiVDKYNVTIFYTAPTLvRSLMRDG-------------------TEYVTRYSRKSL-----RVLGSVGEPINPSAW 455
Cdd:cd05932 220 ------VQRARPTLFFSVPRL-WTKFQQGvqdkipqqklnlllkipvvNSLVKRKVLKGLgldqcRLAGCGSAPVPPALL 292
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 456 RWFYNIvgdsRCPISDTWWQTETGGFMITPLPGAwpQKPGSATFPFFGVQPVIVDEkgqeiegecsGYLCIKKswPGAFR 535
Cdd:cd05932 293 EWYRSL----GLNILEAYGMTENFAYSHLNYPGR--DKIGTVGNAGPGVEVRISED----------GEILVRS--PALMM 354
|
410 420 430 440 450 460 470
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 242061796 536 TLYGDHERYETTYFKPfaGYYFTGDGCSRDKDGYHWLTGRVDDVINVS-GHRIGTAEVESALVSHPQCAEAAVVG 609
Cdd:cd05932 355 GYYKDPEATAEAFTAD--GFLRTGDKGELDADGNLTITGRVKDIFKTSkGKYVAPAPIENKLAEHDRVEMVCVIG 427
|
|
| hsFATP4_like |
cd05939 |
Fatty acid transport proteins (FATP), including FATP4 and FATP1, and similar proteins; Fatty ... |
159-677 |
9.82e-19 |
|
Fatty acid transport proteins (FATP), including FATP4 and FATP1, and similar proteins; Fatty acid transport protein (FATP) transports long-chain or very-long-chain fatty acids across the plasma membrane. At least five copies of FATPs are identified in mammalian cells. This family includes FATP4, FATP1, and homologous proteins. Each FATP has unique patterns of tissue distribution. FATP4 is mainly expressed in the brain, testis, colon and kidney. FATPs also have fatty acid CoA synthetase activity, thus playing dual roles as fatty acid transporters and its activation enzymes. FATPs are the key players in the trafficking of exogenous fatty acids into the cell and in intracellular fatty acid homeostasis.
Pssm-ID: 341262 [Multi-domain] Cd Length: 474 Bit Score: 89.79 E-value: 9.82e-19
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 159 DGKLTYSELLEKVCQLANYLKSLGVGKGDAVIIYLPMLLELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPKLVI 238
Cdd:cd05939 1 DRHWTFRELNEYSNKVANFFQAQGYRSGDVVALFMENRLEFVALWLGLAKIGVETALINSNLRLESLLHCITVSKAKALI 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 239 TcnavkrgvkpiflkDIVDVALVESEKNgvsvglcltyenqsamkredtkwqperdvwwqdvvtkfPTKCDVewVDAEDP 318
Cdd:cd05939 81 F--------------NLLDPLLTQSSTE--------------------------------------PPSQDD--VNFRDK 106
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 319 LFLLYTSGSTGKPKGVLHTSGGYMVYTATTFkYAFDYKPTDI-YWC-----TAdcGWITGHSYVtygpLLNGATVLV--- 389
Cdd:cd05939 107 LFYIYTSGTTGLPKAAVIVHSRYYRIAAGAY-YAFGMRPEDVvYDClplyhSA--GGIMGVGQA----LLHGSTVVIrkk 179
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 390 FEGTpnypdsgRCWDIVDKYNVTIFYTAPTLVRSLMrdGTEYVTRYSRKSLRVLgsVGEPINPSAWRWFYN--------- 460
Cdd:cd05939 180 FSAS-------NFWDDCVKYNCTIVQYIGEICRYLL--AQPPSEEEQKHNVRLA--VGNGLRPQIWEQFVRrfgipqige 248
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 461 IVGDSRCPISDTWWQTETG--GFMITPLPGAWPqkpgsatfpffgVQPVIVDEKGQEIEGECSGyLCI--KKSWPGAF-- 534
Cdd:cd05939 249 FYGATEGNSSLVNIDNHVGacGFNSRILPSVYP------------IRLIKVDEDTGELIRDSDG-LCIpcQPGEPGLLvg 315
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 535 --------RTLYG------DHERYETTYFKPFAGYYFTGDGCSRDKDGYHWLTGRVDDVINVSGHRIGTAEVESALVSHP 600
Cdd:cd05939 316 kiiqndplRRFDGyvnegaTNKKIARDVFKKGDSAFLSGDVLVMDELGYLYFKDRTGDTFRWKGENVSTTEVEGILSNVL 395
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 601 QCAEAAVVGVE-HEVKGQ----GIYAFVTLVDGVPYSEELRKSLimtvrnqiGAFAAPDKIHWAPGLPKTRSGKIMRRIL 675
Cdd:cd05939 396 GLEDVVVYGVEvPGVEGRagmaAIVDPERKVDLDRFSAVLAKSL--------PPYARPQFIRLLPEVDKTGTFKLQKTDL 467
|
..
gi 242061796 676 RK 677
Cdd:cd05939 468 QK 469
|
|
| PRK12467 |
PRK12467 |
peptide synthase; Provisional |
162-684 |
1.30e-18 |
|
peptide synthase; Provisional
Pssm-ID: 237108 [Multi-domain] Cd Length: 3956 Bit Score: 91.38 E-value: 1.30e-18
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 162 LTYSELLEKVCQLANYLKSLGVGKGDAVIIYLPMLLELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPKLVITCN 241
Cdd:PRK12467 538 LSYAELNRQANRLAHVLIAAGVGPDVLVGIAVERSIEMVVGLLAVLKAGGAYVPLDPEYPQDRLAYMLDDSGVRLLLTQS 617
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 242 AvkrgvkpifLKDIVDVAlvesekNGVSVgLCLTyenqsamkredtkwQPERdvWWQDVVTKFPTKCdvewVDAEDPLFL 321
Cdd:PRK12467 618 H---------LLAQLPVP------AGLRS-LCLD--------------EPAD--LLCGYSGHNPEVA----LDPDNLAYV 661
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 322 LYTSGSTGKPKGVLHTSGGYMVYTATTFKYafdykptdiYWCTADCGWITGHSY-------VTYGPLLNGATVLVFeGTP 394
Cdd:PRK12467 662 IYTSGSTGQPKGVAISHGALANYVCVIAER---------LQLAADDSMLMVSTFafdlgvtELFGALASGATLHLL-PPD 731
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 395 NYPDSGRCWDIVDKYNVTIFYTAPTLVRSLMRDG-TEYVTRYSRkslRVLGsvGEPINPSAW-RWFYNIVGdsrCPISDT 472
Cdd:PRK12467 732 CARDAEAFAALMADQGVTVLKIVPSHLQALLQASrVALPRPQRA---LVCG--GEALQVDLLaRVRALGPG---ARLINH 803
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 473 WWQTETG-GFMITPLPGAwPQKPGSATF--PFFGVQPVIVDEKGQEIEGECSGYLCIkkSWPGAFRtlyGDHERYETT-- 547
Cdd:PRK12467 804 YGPTETTvGVSTYELSDE-ERDFGNVPIgqPLANLGLYILDHYLNPVPVGVVGELYI--GGAGLAR---GYHRRPALTae 877
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 548 YF--KPFAG----YYFTGDGCSRDKDGYHWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAAVVGVEHEVKGQGI-Y 620
Cdd:PRK12467 878 RFvpDPFGAdggrLYRTGDLARYRADGVIEYLGRMDHQVKIRGFRIELGEIEARLLAQPGVREAVVLAQPGDAGLQLVaY 957
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....
gi 242061796 621 AFVTLVDGVPYSEELRKSLIMTVRNQIGAFAAPDKIHWAPGLPKTRSGKIMRRILRKIAARQLD 684
Cdd:PRK12467 958 LVPAAVADGAEHQATRDELKAQLRQVLPDYMVPAHLLLLDSLPLTPNGKLDRKALPKPDASAVQ 1021
|
|
| FACL_like_2 |
cd05917 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ... |
508-676 |
4.04e-18 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341241 [Multi-domain] Cd Length: 349 Bit Score: 86.56 E-value: 4.04e-18
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 508 IVDEKGQE-----IEGE--CSGYLCIKKSWpgafrtlyGDHERYETTYFKpfAGYYFTGDGCSRDKDGYHWLTGRVDDVI 580
Cdd:cd05917 187 IVDPEGGIvppvgVPGElcIRGYSVMKGYW--------NDPEKTAEAIDG--DGWLHTGDLAVMDEDGYCRIVGRIKDMI 256
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 581 NVSGHRIGTAEVESALVSHPQCAEAAVVGVEHEVKGQGIYAFVTLVDGVPYSEElrkSLIMTVRNQIGAFAAPDKIHWAP 660
Cdd:cd05917 257 IRGGENIYPREIEEFLHTHPKVSDVQVVGVPDERYGEEVCAWIRLKEGAELTEE---DIKAYCKGKIAHYKVPRYVFFVD 333
|
170
....*....|....*.
gi 242061796 661 GLPKTRSGKIMRRILR 676
Cdd:cd05917 334 EFPLTVSGKIQKFKLR 349
|
|
| entF |
PRK10252 |
enterobactin non-ribosomal peptide synthetase EntF; |
161-675 |
1.30e-17 |
|
enterobactin non-ribosomal peptide synthetase EntF;
Pssm-ID: 236668 [Multi-domain] Cd Length: 1296 Bit Score: 87.79 E-value: 1.30e-17
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 161 KLTYSELLEKVCQLANYLKSLGVGKGDAVIIYLPMLLELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPKLVITc 240
Cdd:PRK10252 483 QFSYREMREQVVALANLLRERGVKPGDSVAVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLIT- 561
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 241 navkrgvkpiflkdivdVALVESEKNGVSVGLCLTYENQSAmkreDTKWQPERdvwwqdvvtkfPTKCDvewvdaeDPLF 320
Cdd:PRK10252 562 -----------------TADQLPRFADVPDLTSLCYNAPLA----PQGAAPLQ-----------LSQPH-------HTAY 602
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 321 LLYTSGSTGKPKGVL--HT---------------SGGYMVYTATtfKYAFDYKPTDIYWctadcgwitghsyvtygPLLN 383
Cdd:PRK10252 603 IIFTSGSTGRPKGVMvgQTaivnrllwmqnhyplTADDVVLQKT--PCSFDVSVWEFFW-----------------PFIA 663
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 384 GATVLVFEgtpnyPDSGR----CWDIVDKYNVTIFYTAPTLVRSLMRDGTEYVTRYSRKSLRVLGSVGEPINPSAWRWFY 459
Cdd:PRK10252 664 GAKLVMAE-----PEAHRdplaMQQFFAEYGVTTTHFVPSMLAAFVASLTPEGARQSCASLRQVFCSGEALPADLCREWQ 738
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 460 --------NIVGDSRCPISDTWWqtetggfmitPLPGAWPQKPGSAT----FPFFGVQPVIVDEKGQEIEGECSGYLCIk 527
Cdd:PRK10252 739 qltgaplhNLYGPTEAAVDVSWY----------PAFGEELAAVRGSSvpigYPVWNTGLRILDARMRPVPPGVAGDLYL- 807
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 528 kswpGAFRTLYGDHERYETTY-------FKPFAGYYFTGDGCSRDKDGYHWLTGRVDDVINVSGHRIGTAEVESALVSHP 600
Cdd:PRK10252 808 ----TGIQLAQGYLGRPDLTAsrfiadpFAPGERMYRTGDVARWLDDGAVEYLGRSDDQLKIRGQRIELGEIDRAMQALP 883
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 601 QCAEAAVVGVE--HEVKGQG----IYAFVTLVDGVPY-SEELRKSLimtvRNQIGAFAAPDKIHWAPGLPKTRSGKIMRR 673
Cdd:PRK10252 884 DVEQAVTHACVinQAAATGGdarqLVGYLVSQSGLPLdTSALQAQL----RERLPPHMVPVVLLQLDQLPLSANGKLDRK 959
|
..
gi 242061796 674 IL 675
Cdd:PRK10252 960 AL 961
|
|
| PRK05852 |
PRK05852 |
fatty acid--CoA ligase family protein; |
162-673 |
2.36e-17 |
|
fatty acid--CoA ligase family protein;
Pssm-ID: 235625 [Multi-domain] Cd Length: 534 Bit Score: 85.71 E-value: 2.36e-17
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 162 LTYSELLEKVCQLANYLKSLGVGKGDAVIIYLPMLLELPIAMLACARIGAVhsvvfagfsadslaqrIVDCKPKLVITCN 241
Cdd:PRK05852 44 ISYRDLARLVDDLAGQLTRSGLLPGDRVALRMGSNAEFVVALLAASRADLV----------------VVPLDPALPIAEQ 107
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 242 AVKRGVKPiflkdiVDVALVESEKNG-------------VSVGLCLTYENQSAMKREDTKWQPerdvwwqDVVTKFPtkc 308
Cdd:PRK05852 108 RVRSQAAG------ARVVLIDADGPHdraepttrwwpltVNVGGDSGPSGGTLSVHLDAATEP-------TPATSTP--- 171
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 309 dvEWVDAEDPLfLLYTSGSTGKPKGVLHTSGGyMVYTATTFKYAFDYKPTDIywCTADCGWITGHSYVT--YGPLLNGAT 386
Cdd:PRK05852 172 --EGLRPDDAM-IMFTGGTTGLPKMVPWTHAN-IASSVRAIITGYRLSPRDA--TVAVMPLYHGHGLIAalLATLASGGA 245
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 387 VLVfegtpnyPDSGRC-----WDIVDKYNVTIFYTAPTLVRSLMRDGTEYVTRYSRKSLRVLGSVGEPINPSAWRWFYNI 461
Cdd:PRK05852 246 VLL-------PARGRFsahtfWDDIKAVGATWYTAVPTIHQILLERAATEPSGRKPAALRFIRSCSAPLTAETAQALQTE 318
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 462 VGdsrCPISDTWWQTET---------GGFMITPLPGAWPQKPGSATfpffGVQPVIVDEKGQEIEGECSGYLCIkkSWPG 532
Cdd:PRK05852 319 FA---APVVCAFGMTEAthqvtttqiEGIGQTENPVVSTGLVGRST----GAQIRIVGSDGLPLPAGAVGEVWL--RGTT 389
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 533 AFRTLYGDHERYETTYFKpfaGYYFTGDGCSRDKDGYHWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAAVVGVEH 612
Cdd:PRK05852 390 VVRGYLGDPTITAANFTD---GWLRTGDLGSLSAAGDLSIRGRIKELINRGGEKISPERVEGVLASHPNVMEAAVFGVPD 466
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|.
gi 242061796 613 EVKGQGIYAFVTLVDGVPYSeelRKSLIMTVRNQIGAFAAPDKIHWAPGLPKTRSGKIMRR 673
Cdd:PRK05852 467 QLYGEAVAAVIVPRESAPPT---AEELVQFCRERLAAFEIPASFQEASGLPHTAKGSLDRR 524
|
|
| PRK05691 |
PRK05691 |
peptide synthase; Validated |
145-675 |
2.44e-17 |
|
peptide synthase; Validated
Pssm-ID: 235564 [Multi-domain] Cd Length: 4334 Bit Score: 87.15 E-value: 2.44e-17
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 145 DKIAMYWEGnepsqdGKLTYSELLEKVCQLANYLKSLGVGKGDAVIIYLPMLLELPIAMLACARIGAVHSVVFAGFSADS 224
Cdd:PRK05691 1146 ERIALVWDG------GSLDYAELHAQANRLAHYLRDKGVGPDVCVAIAAERSPQLLVGLLAILKAGGAYVPLDPDYPAER 1219
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 225 LAQRIVDCKPKLVITCNAVKRGVkpiflkdivdvalveSEKNGVSVgLCLTyenqsamkredtkwQPERDVWwqdvvtkf 304
Cdd:PRK05691 1220 LAYMLADSGVELLLTQSHLLERL---------------PQAEGVSA-IALD--------------SLHLDSW-------- 1261
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 305 PTKCDVEWVDAEDPLFLLYTSGSTGKPKGVLHTSGG------YMVYTattfkYAFDykPTDIYWCTA---------DCGW 369
Cdd:PRK05691 1262 PSQAPGLHLHGDNLAYVIYTSGSTGQPKGVGNTHAAlaerlqWMQAT-----YALD--DSDVLMQKApisfdvsvwECFW 1334
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 370 itghsyvtygPLLNGATvLVFEGTPNYPDSGRCWDIVDKYNVTIFYTAPTLVRSLMRDGTEYVTRysrkSLRVLGSVGEP 449
Cdd:PRK05691 1335 ----------PLITGCR-LVLAGPGEHRDPQRIAELVQQYGVTTLHFVPPLLQLFIDEPLAAACT----SLRRLFSGGEA 1399
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 450 InPSAWR----------WFYNIVGDSRCPISDTWWQTETGGFMITPLpgawpqkpGSatfPFFGVQPVIVDEKGQEIEGE 519
Cdd:PRK05691 1400 L-PAELRnrvlqrlpqvQLHNRYGPTETAINVTHWQCQAEDGERSPI--------GR---PLGNVLCRVLDAELNLLPPG 1467
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 520 CSGYLCIKKSwpGAFRTLYG----DHERY-ETTYFKPFAGYYFTGDGCSRDKDGYHWLTGRVDDVINVSGHRIGTAEVES 594
Cdd:PRK05691 1468 VAGELCIGGA--GLARGYLGrpalTAERFvPDPLGEDGARLYRTGDRARWNADGALEYLGRLDQQVKLRGFRVEPEEIQA 1545
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 595 ALVSHPQCAEAAVVgVEHEVKGQGIYAFVTLVDGvpySEELRKSLIMTVRNQIGAFAAPDKIHWAPGLPKTRSGKIMRRI 674
Cdd:PRK05691 1546 RLLAQPGVAQAAVL-VREGAAGAQLVGYYTGEAG---QEAEAERLKAALAAELPEYMVPAQLIRLDQMPLGPSGKLDRRA 1621
|
.
gi 242061796 675 L 675
Cdd:PRK05691 1622 L 1622
|
|
| FATP_FACS |
cd05940 |
Fatty acid transport proteins (FATP) play dual roles as fatty acid transporters and its ... |
159-677 |
3.37e-17 |
|
Fatty acid transport proteins (FATP) play dual roles as fatty acid transporters and its activation enzymes; Fatty acid transport protein (FATP) transports long-chain or very-long-chain fatty acids across the plasma membrane. FATPs also have fatty acid CoA synthetase activity, thus playing dual roles as fatty acid transporters and its activation enzymes. At least five copies of FATPs are identified in mammalian cells. This family also includes prokaryotic FATPs. FATPs are the key players in the trafficking of exogenous fatty acids into the cell and in intracellular fatty acid homeostasis.
Pssm-ID: 341263 [Multi-domain] Cd Length: 449 Bit Score: 84.71 E-value: 3.37e-17
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 159 DGKLTYSELLEKVCQLANYLKSLGVGKGDAVIIYLPMLLELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPKLVI 238
Cdd:cd05940 1 DEALTYAELDAMANRYARWLKSLGLKPGDVVALFMENRPEYVLLWLGLVKIGAVAALINYNLRGESLAHCLNVSSAKHLV 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 239 TcnavkrgvkpiflkdivdvalvesekngvsvglcltyenqsamkredtkwqperdvwwqdvvtkfptkcdvewvdaeDP 318
Cdd:cd05940 81 V-----------------------------------------------------------------------------DA 83
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 319 LFLLYTSGSTGKPKGVLHTSGGYMVYTATtFKYAFDYKPTDIYWCTADCGWITGHSYVTYGPLLNGATVLV---FEGTpn 395
Cdd:cd05940 84 ALYIYTSGTTGLPKAAIISHRRAWRGGAF-FAGSGGALPSDVLYTCLPLYHSTALIVGWSACLASGATLVIrkkFSAS-- 160
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 396 ypdsgRCWDIVDKYNVTIFYTAPTLVRSLMRDGTEYVTRysRKSLRVLgsVGEPINPSAWRWFYNIVGDSRcpISDTWWQ 475
Cdd:cd05940 161 -----NFWDDIRKYQATIFQYIGELCRYLLNQPPKPTER--KHKVRMI--FGNGLRPDIWEEFKERFGVPR--IAEFYAA 229
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 476 TE--TGGFMITPLPGAWPQKPgSATFPFFGVQPVIVD-EKGQEIEGEcSGYlCIK--KSWPGAFRTLYGDHERYETtYFK 550
Cdd:cd05940 230 TEgnSGFINFFGKPGAIGRNP-SLLRKVAPLALVKYDlESGEPIRDA-EGR-CIKvpRGEPGLLISRINPLEPFDG-YTD 305
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 551 PFA--------------GYYFTGDGCSRDKDGYHWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAAVVGVE---HE 613
Cdd:cd05940 306 PAAtekkilrdvfkkgdAWFNTGDLMRLDGEGFWYFVDRLGDTFRWKGENVSTTEVAAVLGAFPGVEEANVYGVQvpgTD 385
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....
gi 242061796 614 VKGqGIYAfvtLVDGVPYSEELrKSLIMTVRNQIGAFAAPDKIHWAPGLPKTRSGKIMRRILRK 677
Cdd:cd05940 386 GRA-GMAA---IVLQPNEEFDL-SALAAHLEKNLPGYARPLFLRLQPEMEITGTFKQQKVDLRN 444
|
|
| PRK13383 |
PRK13383 |
acyl-CoA synthetase; Provisional |
440-675 |
1.03e-16 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 139531 [Multi-domain] Cd Length: 516 Bit Score: 83.89 E-value: 1.03e-16
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 440 LRVLGSVGEPINPSAWRWFYNIVGDSrcpISDTWWQTETG-GFMITPLP-GAWPQKPGSatfPFFGVQPVIVDEKGQEIE 517
Cdd:PRK13383 294 LRVVMSSGDRLDPTLGQRFMDTYGDI---LYNGYGSTEVGiGALATPADlRDAPETVGK---PVAGCPVRILDRNNRPVG 367
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 518 GECSGYLCIKKSWPGafrtlygdhERYETTYFKPFA-GYYFTGDGCSRDKDGYHWLTGRVDDVINVSGHRIGTAEVESAL 596
Cdd:PRK13383 368 PRVTGRIFVGGELAG---------TRYTDGGGKAVVdGMTSTGDMGYLDNAGRLFIVGREDDMIISGGENVYPRAVENAL 438
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 597 VSHPQCAEAAVVGVEHEVKGQGIYAFVTLVDGVPY-SEELRKSLimtvRNQIGAFAAPDKIHWAPGLPKTRSGKIMRRIL 675
Cdd:PRK13383 439 AAHPAVADNAVIGVPDERFGHRLAAFVVLHPGSGVdAAQLRDYL----KDRVSRFEQPRDINIVSSIPRNPTGKVLRKEL 514
|
|
| PRK07059 |
PRK07059 |
Long-chain-fatty-acid--CoA ligase; Validated |
160-676 |
2.80e-16 |
|
Long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 235923 [Multi-domain] Cd Length: 557 Bit Score: 82.38 E-value: 2.80e-16
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 160 GK-LTYSELLEKVCQLANYLKSLGVGKGDAVIIYLPMLLELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPKLVI 238
Cdd:PRK07059 46 GKaITYGELDELSRALAAWLQSRGLAKGARVAIMMPNVLQYPVAIAAVLRAGYVVVNVNPLYTPRELEHQLKDSGAEAIV 125
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 239 TCNAVKRGVKPIFLKDIVDVALVESEKN-----GVSVGLCLtyenqSAMKREDTKWQPERDVWWQDVVT-----KF-PTK 307
Cdd:PRK07059 126 VLENFATTVQQVLAKTAVKHVVVASMGDllgfkGHIVNFVV-----RRVKKMVPAWSLPGHVRFNDALAegarqTFkPVK 200
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 308 cdvewVDAEDPLFLLYTSGSTGKPKG--VLHTS-GGYMVYTATTFKYAFDYKPTD-------------IYWCTAdCGWIt 371
Cdd:PRK07059 201 -----LGPDDVAFLQYTGGTTGVSKGatLLHRNiVANVLQMEAWLQPAFEKKPRPdqlnfvcalplyhIFALTV-CGLL- 273
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 372 ghsyvtyGPLLNGATVLVfegtPNYPDSGRCWDIVDKYNVTIFYTAPTLVRSLMRdgTEYVTRYSRKSLRV-LG---SVG 447
Cdd:PRK07059 274 -------GMRTGGRNILI----PNPRDIPGFIKELKKYQVHIFPAVNTLYNALLN--NPDFDKLDFSKLIVaNGggmAVQ 340
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 448 EPInpsAWRWfYNIVGdsrCPISDTWWQTETGGfMITPLPGAWPQKPGSATFPFFGVQPVIVDEKGQEIE-GEcSGYLCI 526
Cdd:PRK07059 341 RPV---AERW-LEMTG---CPITEGYGLSETSP-VATCNPVDATEFSGTIGLPLPSTEVSIRDDDGNDLPlGE-PGEICI 411
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 527 KKSwpgafRTLYGDHERYETTYFKPFA-GYYFTGDGCSRDKDGYHWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEA 605
Cdd:PRK07059 412 RGP-----QVMAGYWNRPDETAKVMTAdGFFRTGDVGVMDERGYTKIVDRKKDMILVSGFNVYPNEIEEVVASHPGVLEV 486
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 242061796 606 AVVGVEHEVKGQGIYAFVTLVDGVPYSEELRKSlimtVRNQIGAFAAPDKIHWAPGLPKTRSGKIMRRILR 676
Cdd:PRK07059 487 AAVGVPDEHSGEAVKLFVVKKDPALTEEDVKAF----CKERLTNYKRPKFVEFRTELPKTNVGKILRRELR 553
|
|
| FadD3 |
cd17638 |
acyl-CoA synthetase FadD3 and similar proteins; This family contains long chain fatty acid CoA ... |
321-672 |
3.77e-16 |
|
acyl-CoA synthetase FadD3 and similar proteins; This family contains long chain fatty acid CoA ligases, including FadD3 which is an acyl-CoA synthetase that initiates catabolism of cholesterol rings C and D in actinobacteria. The cholesterol catabolic pathway occurs in most mycolic acid-containing actinobacteria, such as Rhodococcus jostii RHA1, and is critical for Mycobacterium tuberculosis (Mtb) during infection. FadD3 catalyzes the ATP-dependent CoA thioesterification of 3a-alpha-H-4alpha(3'-propanoate)-7a-beta-methylhexahydro-1,5-indanedione (HIP) to yield HIP-CoA. Hydroxylated analogs of HIP, 5alpha-OH HIP and 1beta-OH HIP, can also be used.
Pssm-ID: 341293 [Multi-domain] Cd Length: 330 Bit Score: 80.24 E-value: 3.77e-16
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 321 LLYTSGSTGKPKGVLhtsggyMVYTATTFKYAfdykptdiYWCtaDCGWIT-GHSYVTYGP--------------LLNGA 385
Cdd:cd17638 5 IMFTSGTTGRSKGVM------CAHRQTLRAAA--------AWA--DCADLTeDDRYLIINPffhtfgykagivacLLTGA 68
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 386 TVL---VFegtpnypDSGRCWDIVDKYNVTIFYTAPTLVRSLMrdGTEYVTRYSRKSLRVLGSVGEPINPSAWRWFYNIV 462
Cdd:cd17638 69 TVVpvaVF-------DVDAILEAIERERITVLPGPPTLFQSLL--DHPGRKKFDLSSLRAAVTGAATVPVELVRRMRSEL 139
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 463 GDSRcpISDTWWQTETG-GFMITPLPGAWPQKPGSATfPFFGVQPVIVDEKGQEIEGE--CSGYLCIKKSwpgAFRTLYG 539
Cdd:cd17638 140 GFET--VLTAYGLTEAGvATMCRPGDDAETVATTCGR-ACPGFEVRIADDGEVLVRGYnvMQGYLDDPEA---TAEAIDA 213
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 540 DheryettyfkpfaGYYFTGDGCSRDKDGYHWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAAVVGVEHEVKGQGI 619
Cdd:cd17638 214 D-------------GWLHTGDVGELDERGYLRITDRLKDMYIVGGFNVYPAEVEGALAEHPGVAQVAVIGVPDERMGEVG 280
|
330 340 350 360 370
....*....|....*....|....*....|....*....|....*....|...
gi 242061796 620 YAFVTLVDGVPYSEElrkSLIMTVRNQIGAFAAPDKIHWAPGLPKTRSGKIMR 672
Cdd:cd17638 281 KAFVVARPGVTLTEE---DVIAWCRERLANYKVPRFVRFLDELPRNASGKVMK 330
|
|
| PRK13388 |
PRK13388 |
acyl-CoA synthetase; Provisional |
313-676 |
5.08e-16 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 237374 [Multi-domain] Cd Length: 540 Bit Score: 81.61 E-value: 5.08e-16
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 313 VDAEDPLFLLYTSGSTGKPKGVLHTSG-----GYmvytATTFKYafDYKPTDIYWCTADcgwiTGHS---YVTYGPLL-N 383
Cdd:PRK13388 147 VDAMDPFMLIFTSGTTGAPKAVRCSHGrlafaGR----ALTERF--GLTRDDVCYVSMP----LFHSnavMAGWAPAVaS 216
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 384 GATVLVfegTPNYPDSGRCWDiVDKYNVTIF--------YTAPTLVRSLMRDGTeyvtrysrksLRVlgSVGEPINPSAW 455
Cdd:PRK13388 217 GAAVAL---PAKFSASGFLDD-VRRYGATYFnyvgkplaYILATPERPDDADNP----------LRV--AFGNEASPRDI 280
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 456 RWFYNIVGdsrCPISDTWWQTETGGfMITPLPGAwPqkPGSATFPFFGVQ-----------PVIVDEKGQEIEG-ECSGY 523
Cdd:PRK13388 281 AEFSRRFG---CQVEDGYGSSEGAV-IVVREPGT-P--PGSIGRGAPGVAiynpetltecaVARFDAHGALLNAdEAIGE 353
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 524 LcIKKSWPGAFRTLYGDHEryeTTYFKPFAGYYFTGDGCSRDKDGYHWLTGRVDDVINVSGHRIGTAEVESALVSHPQCA 603
Cdd:PRK13388 354 L-VNTAGAGFFEGYYNNPE---ATAERMRHGMYWSGDLAYRDADGWIYFAGRTADWMRVDGENLSAAPIERILLRHPAIN 429
|
330 340 350 360 370 380 390
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 242061796 604 EAAVVGVEHEVKGQGIYAFVTLVDGVPYSEElrkSLIMTVRNQ--IGAFAAPDKIHWAPGLPKTRSGKIMRRILR 676
Cdd:PRK13388 430 RVAVYAVPDERVGDQVMAALVLRDGATFDPD---AFAAFLAAQpdLGTKAWPRYVRIAADLPSTATNKVLKRELI 501
|
|
| PLN02479 |
PLN02479 |
acetate-CoA ligase |
554-689 |
6.40e-16 |
|
acetate-CoA ligase
Pssm-ID: 178097 [Multi-domain] Cd Length: 567 Bit Score: 81.43 E-value: 6.40e-16
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 554 GYYFTGDGCSRDKDGYHWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAAVVGVEHEVKGQGIYAFVTLVDGVPYSE 633
Cdd:PLN02479 430 GWFHSGDLGVKHPDGYIEIKDRSKDIIISGGENISSLEVENVVYTHPAVLEASVVARPDERWGESPCAFVTLKPGVDKSD 509
|
90 100 110 120 130
....*....|....*....|....*....|....*....|....*....|....*...
gi 242061796 634 E--LRKSLIMTVRNQIGAFAAPDKIHWAPgLPKTRSGKIMRRILRKIAarqlDELGDI 689
Cdd:PLN02479 510 EaaLAEDIMKFCRERLPAYWVPKSVVFGP-LPKTATGKIQKHVLRAKA----KEMGPV 562
|
|
| PRK08751 |
PRK08751 |
long-chain fatty acid--CoA ligase; |
162-680 |
7.32e-16 |
|
long-chain fatty acid--CoA ligase;
Pssm-ID: 181546 [Multi-domain] Cd Length: 560 Bit Score: 81.08 E-value: 7.32e-16
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 162 LTYSELLEKVCQLANYL-KSLGVGKGDAVIIYLPMLLELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPKLVIT- 239
Cdd:PRK08751 51 ITYREADQLVEQFAAYLlGELQLKKGDRVALMMPNCLQYPIATFGVLRAGLTVVNVNPLYTPRELKHQLIDSGASVLVVi 130
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 240 ---CNAVKRGVKPIFLKDIVDVALVE--SEKNGVSVGLCLTYenqsaMKREDTKWQPERDVWWQDVVT-----KFPTkcd 309
Cdd:PRK08751 131 dnfGTTVQQVIADTPVKQVITTGLGDmlGFPKAALVNFVVKY-----VKKLVPEYRINGAIRFREALAlgrkhSMPT--- 202
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 310 vEWVDAEDPLFLLYTSGSTGKPKGVLHTSGGYMVYTATTFKyafdykptdiyWCTADCGWITGHSYV-TYGPLL------ 382
Cdd:PRK08751 203 -LQIEPDDIAFLQYTGGTTGVAKGAMLTHRNLVANMQQAHQ-----------WLAGTGKLEEGCEVViTALPLYhifalt 270
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 383 -NGATVLVFEGTPNYPDSGRcwDI------VDKYNVTIFYTAPTLVRSLMrdGTEYVTRYSRKSLRVLGSVGEPINPS-A 454
Cdd:PRK08751 271 aNGLVFMKIGGCNHLISNPR--DMpgfvkeLKKTRFTAFTGVNTLFNGLL--NTPGFDQIDFSSLKMTLGGGMAVQRSvA 346
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 455 WRWfYNIVGdsrCPISDTWWQTETG-GFMITPLpgAWPQKPGSATFPFFGVQPVIVDEKGQEIE-GECsGYLCIKKSwpg 532
Cdd:PRK08751 347 ERW-KQVTG---LTLVEAYGLTETSpAACINPL--TLKEYNGSIGLPIPSTDACIKDDAGTVLAiGEI-GELCIKGP--- 416
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 533 afRTLYGDHER-YETTYFKPFAGYYFTGDGCSRDKDGYHWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAAVVGVE 611
Cdd:PRK08751 417 --QVMKGYWKRpEETAKVMDADGWLHTGDIARMDEQGFVYIVDRKKDMILVSGFNVYPNEIEDVIAMMPGVLEVAAVGVP 494
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 242061796 612 HEVKGQGIYAFVTLVDGVPYSEELRKSlimtVRNQIGAFAAPDKIHWAPGLPKTRSGKIMRRILRKIAA 680
Cdd:PRK08751 495 DEKSGEIVKVVIVKKDPALTAEDVKAH----ARANLTGYKQPRIIEFRKELPKTNVGKILRRELRDAAK 559
|
|
| A_NRPS_LgrA-like |
cd17645 |
adenylation (A) domain of linear gramicidin synthetase (LgrA) and similar proteins; This ... |
145-675 |
7.61e-16 |
|
adenylation (A) domain of linear gramicidin synthetase (LgrA) and similar proteins; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes linear gramicidin synthetase (LgrA) in Brevibacillus brevis. LgrA has a formylation domain fused to the N-terminal end that formylates its substrate for linear gramicidin synthesis to proceed. This formyl group is essential for the clinically important antibacterial activity of gramicidin by enabling head-to-head gramicidin dimers to make a beta-helical pore in gram-positive bacterial membranes, allowing free passage of monovalent cations, destroying the ion gradient and killing bacteria. This family also includes bacitracin synthetase 1 (known as ATP-dependent cysteine adenylase or BA1); it activates cysteine, incorporates two D-amino acids, releases and cyclizes the mature bacitracin, an antibiotic that is a mixture of related cyclic peptides that disrupt gram positive bacteria by interfering with cell wall and peptidoglycan synthesis. Also included is surfactin synthetase which activates and polymerizes the amino acids Leu, Glu, Asp, and Val to form the antibiotic surfactin.
Pssm-ID: 341300 [Multi-domain] Cd Length: 440 Bit Score: 80.68 E-value: 7.61e-16
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 145 DKIAMYWEGNepsqdgKLTYSELLEKVCQLANYLKSLGVGKGDAVIIYLPMLLELPIAMLACARIGAVHSVVFAGFSADS 224
Cdd:cd17645 13 DHVAVVDRGQ------SLTYKQLNEKANQLARHLRGKGVKPDDQVGIMLDKSLDMIAAILGVLKAGGAYVPIDPDYPGER 86
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 225 LAQRIVDCKPKLVITcnavkrgvkpiflkdivdvalvesekngvsvglcltyenqsamkredtkwqperdvwwqdvvtkf 304
Cdd:cd17645 87 IAYMLADSSAKILLT----------------------------------------------------------------- 101
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 305 ptkcdvewvDAEDPLFLLYTSGSTGKPKGVL--HTSggyMVYTATTFKYAFDYKPTDIYWCTADCGWiTGHSYVTYGPLL 382
Cdd:cd17645 102 ---------NPDDLAYVIYTSGSTGLPKGVMieHHN---LVNLCEWHRPYFGVTPADKSLVYASFSF-DASAWEIFPHLT 168
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 383 NGATVLVFEGTPNYpDSGRCWDIVDKYNVTIFYTaPTLVrslmrdgTEYVTRYSRKSLRVLGSVGEPINPSAWRWF--YN 460
Cdd:cd17645 169 AGAALHVVPSERRL-DLDALNDYFNQEGITISFL-PTGA-------AEQFMQLDNQSLRVLLTGGDKLKKIERKGYklVN 239
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 461 IVGDSRCPIsdtwwqtetggfMITPLPGAWPQKPGSATFPFFGVQPVIVDEKGQEIEGECSGYLCIKKSwpGAFRTLYG- 539
Cdd:cd17645 240 NYGPTENTV------------VATSFEIDKPYANIPIGKPIDNTRVYILDEALQLQPIGVAGELCIAGE--GLARGYLNr 305
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 540 ---DHERYETTYFKPFAGYYFTGDGCSRDKDGYHWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAAVVGVEHEVKG 616
Cdd:cd17645 306 pelTAEKFIVHPFVPGERMYRTGDLAKFLPDGNIEFLGRLDQQVKIRGYRIEPGEIEPFLMNHPLIELAAVLAKEDADGR 385
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|....*....
gi 242061796 617 QGIYAFVTLVDGVPYsEELRKSLimtvRNQIGAFAAPDKIHWAPGLPKTRSGKIMRRIL 675
Cdd:cd17645 386 KYLVAYVTAPEEIPH-EELREWL----KNDLPDYMIPTYFVHLKALPLTANGKVDRKAL 439
|
|
| PtmA |
cd17636 |
long-chain fatty acid CoA ligase (FadD); This family contains fatty acid CoA ligases, ... |
317-668 |
1.68e-15 |
|
long-chain fatty acid CoA ligase (FadD); This family contains fatty acid CoA ligases, including acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II, most of which are yet to be characterized. Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341291 [Multi-domain] Cd Length: 331 Bit Score: 78.11 E-value: 1.68e-15
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 317 DPLFLLYTSGSTGKPKGVLHTSGGYMVYTattfkyafdykptdiyWCTADCGWITGHS-YVTYGPL------LNGATVLV 389
Cdd:cd17636 1 DPVLAIYTAAFSGRPNGALLSHQALLAQA----------------LVLAVLQAIDEGTvFLNSGPLfhigtlMFTLATFH 64
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 390 FEGTPNY---PDSGRCWDIVDKYNVT-IFYTAPTL--VRSLMRDGteyvtRYSRKSLRVLgsvgepinPSAWRWFYNIvg 463
Cdd:cd17636 65 AGGTNVFvrrVDAEEVLELIEAERCThAFLLPPTIdqIVELNADG-----LYDLSSLRSS--------PAAPEWNDMA-- 129
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 464 dsrcPISDTWW--------QTETGGFMITplpgAWPQKPGSATF----PFfgVQPVIVDEKGQEI-EGEcSGYLCIKKsw 530
Cdd:cd17636 130 ----TVDTSPWgrkpggygQTEVMGLATF----AALGGGAIGGAgrpsPL--VQVRILDEDGREVpDGE-VGEIVARG-- 196
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 531 PGAFRTLYGdheRYETTYFKPFAGYYFTGDGCSRDKDGYHWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAAVVGV 610
Cdd:cd17636 197 PTVMAGYWN---RPEVNARRTRGGWHHTNDLGRREPDGSLSFVGPKTRMIKSGAENIYPAEVERCLRQHPAVADAAVIGV 273
|
330 340 350 360 370
....*....|....*....|....*....|....*....|....*....|....*...
gi 242061796 611 EHEVKGQGIYAFVTLVDGVPYSEElrkSLIMTVRNQIGAFAAPDKIHWAPGLPKTRSG 668
Cdd:cd17636 274 PDPRWAQSVKAIVVLKPGASVTEA---ELIEHCRARIASYKKPKSVEFADALPRTAGG 328
|
|
| PRK07008 |
PRK07008 |
long-chain-fatty-acid--CoA ligase; Validated |
305-676 |
1.85e-15 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 235908 [Multi-domain] Cd Length: 539 Bit Score: 79.75 E-value: 1.85e-15
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 305 PTKCDVEWVDAED-----PLF-------LLYTSGSTGKPKGVL--HTSggymvytatTFKYAFDYKPTDIYWCTA-DC-- 367
Cdd:PRK07008 153 PLLCYETLVGAQDgdydwPRFdenqassLCYTSGTTGNPKGALysHRS---------TVLHAYGAALPDAMGLSArDAvl 223
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 368 ---------GW-------ITGHSYVTYGPLLNGATVlvfegtpnypdsgrcWDIVDKYNVTIFYTAPTLVRSLMRDGTEY 431
Cdd:PRK07008 224 pvvpmfhvnAWglpysapLTGAKLVLPGPDLDGKSL---------------YELIEAERVTFSAGVPTVWLGLLNHMREA 288
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 432 VTRYSRKSLRVLGSVGEPinPSAWRWFYNIVGdsrCPISDTWWQTETGGF-MITPLPGAWPQKPGSATF--------PFF 502
Cdd:PRK07008 289 GLRFSTLRRTVIGGSACP--PAMIRTFEDEYG---VEVIHAWGMTEMSPLgTLCKLKWKHSQLPLDEQRkllekqgrVIY 363
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 503 GVQPVIVDEKGQEI--EGECSGYLCIKKSWpgafrtlygdherYETTYFK----PF-AGYYFTGDGCSRDKDGYHWLTGR 575
Cdd:PRK07008 364 GVDMKIVGDDGRELpwDGKAFGDLQVRGPW-------------VIDRYFRgdasPLvDGWFPTGDVATIDADGFMQITDR 430
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 576 VDDVINVSGHRIGTAEVESALVSHPQCAEAAVVGVEHEVKGQGIYAFVTLVDGVpysEELRKSLIMTVRNQIGAFAAPDK 655
Cdd:PRK07008 431 SKDVIKSGGEWISSIDIENVAVAHPAVAEAACIACAHPKWDERPLLVVVKRPGA---EVTREELLAFYEGKVAKWWIPDD 507
|
410 420
....*....|....*....|.
gi 242061796 656 IHWAPGLPKTRSGKIMRRILR 676
Cdd:PRK07008 508 VVFVDAIPHTATGKLQKLKLR 528
|
|
| PRK12492 |
PRK12492 |
long-chain-fatty-acid--CoA ligase; Provisional |
450-679 |
2.24e-15 |
|
long-chain-fatty-acid--CoA ligase; Provisional
Pssm-ID: 171539 [Multi-domain] Cd Length: 562 Bit Score: 79.48 E-value: 2.24e-15
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 450 INPSAWRWfYNIVGdsrCPISDTWWQTETggfmiTPLPGAWP----QKPGSATFPFFGVQPVIVDEKGQEIE-GEcSGYL 524
Cdd:PRK12492 346 VKATAERW-EQLTG---CTIVEGYGLTET-----SPVASTNPygelARLGTVGIPVPGTALKVIDDDGNELPlGE-RGEL 415
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 525 CIKKSwpgafRTLYGDHERYETTYFKPFA-GYYFTGDGCSRDKDGYHWLTGRVDDVINVSGHRIGTAEVESALVSHPQCA 603
Cdd:PRK12492 416 CIKGP-----QVMKGYWQQPEATAEALDAeGWFKTGDIAVIDPDGFVRIVDRKKDLIIVSGFNVYPNEIEDVVMAHPKVA 490
|
170 180 190 200 210 220 230
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 242061796 604 EAAVVGVEHEVKGQGIYAFVTLVDGVPYSEELRKslimTVRNQIGAFAAPDKIHWAPGLPKTRSGKIMRRILRKIA 679
Cdd:PRK12492 491 NCAAIGVPDERSGEAVKLFVVARDPGLSVEELKA----YCKENFTGYKVPKHIVLRDSLPMTPVGKILRRELRDIA 562
|
|
| PRK05677 |
PRK05677 |
long-chain-fatty-acid--CoA ligase; Validated |
162-683 |
2.25e-15 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 168170 [Multi-domain] Cd Length: 562 Bit Score: 79.81 E-value: 2.25e-15
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 162 LTYSELLEKVCQLANYLKSL-GVGKGDAVIIYLPMLLELPIAMLACARIGAVhsVVFAG--FSADSLAQRIVDCKPK-LV 237
Cdd:PRK05677 50 LTYGELYKLSGAFAAWLQQHtDLKPGDRIAVQLPNVLQYPVAVFGAMRAGLI--VVNTNplYTAREMEHQFNDSGAKaLV 127
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 238 ITCNAV--------KRGVKPIFLKDIVDV----------ALVESEKNGVSvglclTYENQSAMKREDT----KWQPERDV 295
Cdd:PRK05677 128 CLANMAhlaekvlpKTGVKHVIVTEVADMlpplkrllinAVVKHVKKMVP-----AYHLPQAVKFNDAlakgAGQPVTEA 202
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 296 WwqdvvtkfptkcdvewVDAEDPLFLLYTSGSTGKPKGVLHTSGGyMVYTATTFKYAFDYKPTD-------------IYW 362
Cdd:PRK05677 203 N----------------PQADDVAVLQYTGGTTGVAKGAMLTHRN-LVANMLQCRALMGSNLNEgceiliaplplyhIYA 265
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 363 CTADCGWITghsyvtygpLLNGATVLVfegtPNYPDSGRCWDIVDKYNVTIFYTAPTLVRSLMRDgtEYVTRYSRKSLRV 442
Cdd:PRK05677 266 FTFHCMAMM---------LIGNHNILI----SNPRDLPAMVKELGKWKFSGFVGLNTLFVALCNN--EAFRKLDFSALKL 330
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 443 LGSVGEPINPSAWRWFYNIVGdsrCPISDTWWQTETggfmiTPLPGAWPQK---PGSATFPFFGVQPVIVDEKGQEIEGE 519
Cdd:PRK05677 331 TLSGGMALQLATAERWKEVTG---CAICEGYGMTET-----SPVVSVNPSQaiqVGTIGIPVPSTLCKVIDDDGNELPLG 402
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 520 CSGYLCIKKSwpgafRTLYGDHERYETTYFKPFA-GYYFTGDGCSRDKDGYHWLTGRVDDVINVSGHRIGTAEVESALVS 598
Cdd:PRK05677 403 EVGELCVKGP-----QVMKGYWQRPEATDEILDSdGWLKTGDIALIQEDGYMRIVDRKKDMILVSGFNVYPNELEDVLAA 477
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 599 HPQCAEAAVVGVEHEVKGQGIYAFVTlvdgVPYSEELRKSLIMT-VRNQIGAFAAPDKIHWAPGLPKTRSGKIMRRILRK 677
Cdd:PRK05677 478 LPGVLQCAAIGVPDEKSGEAIKVFVV----VKPGETLTKEQVMEhMRANLTGYKVPKAVEFRDELPTTNVGKILRRELRD 553
|
....*.
gi 242061796 678 IAARQL 683
Cdd:PRK05677 554 EELKKA 559
|
|
| A_NRPS_acs4 |
cd17654 |
acyl-CoA synthetase family member 4; This family of the adenylation (A) domain of nonribosomal ... |
162-670 |
1.91e-14 |
|
acyl-CoA synthetase family member 4; This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) contains acyl-CoA synthethase family member 4, also known as 2-aminoadipic 6-semialdehyde dehydrogenase or aminoadipate-semialdehyde dehydrogenase, most of which are uncharacterized. Acyl-CoA synthetase catalyzes the initial reaction in fatty acid metabolism, by forming a thioester with CoA. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341309 [Multi-domain] Cd Length: 449 Bit Score: 76.36 E-value: 1.91e-14
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 162 LTYSELLEKVCQLANYLKSLGVGKGDAVIIYLPMLLELPIAMLACARIGAVhsvvFAGFSADSLAQRIVDckpklvitcn 241
Cdd:cd17654 17 VSYADLAEKISNLSNFLRKKFQTEERAIGLRCDRGTESPVAILAILFLGAA----YAPIDPASPEQRSLT---------- 82
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 242 avkrgvkpiFLKDIVDVALVESEKNGVSVGLCLTYENQSAMKredtkwqperdvwwqdvvTKFPTkcdvewvdaedpLFL 321
Cdd:cd17654 83 ---------VMKKCHVSYLLQNKELDNAPLSFTPEHRHFNIR------------------TDECL------------AYV 123
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 322 LYTSGSTGKPKGVL--HTSggyMVYTATTFKYAFDYKPTDIYWCTadcgwitghSYVTYGP--------LLNGATVLVFE 391
Cdd:cd17654 124 IHTSGTTGTPKIVAvpHKC---ILPNIQHFRSLFNITSEDILFLT---------SPLTFDPsvveiflsLSSGATLLIVP 191
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 392 gTPNYPDSGRCWDIVDKYN-VTIFYTAPTLVRSLMRDGTEYVTRYSRKSLRVLGSVGEPInPS-----AWRWFYNivgds 465
Cdd:cd17654 192 -TSVKVLPSKLADILFKRHrITVLQATPTLFRRFGSQSIKSTVLSATSSLRVLALGGEPF-PSlvilsSWRGKGN----- 264
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 466 RCPISDTWWQTETGGFMIT--------PLPGAwpqkpgsatFPFFGVQPVIVDEKGQEIEGECSGYLCIKkswpGAFRtl 537
Cdd:cd17654 265 RTRIFNIYGITEVSCWALAykvpeedsPVQLG---------SPLLGTVIEVRDQNGSEGTGQVFLGGLNR----VCIL-- 329
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 538 ygdhERYETTyfkPFAGYYFTGDGCSRdKDGYHWLTGRVDDVINVSGHRIGTAEVESALVSHPQcAEAAVVGVEhevKGQ 617
Cdd:cd17654 330 ----DDEVTV---PKGTMRATGDFVTV-KDGELFFLGRKDSQIKRRGKRINLDLIQQVIESCLG-VESCAVTLS---DQQ 397
|
490 500 510 520 530
....*....|....*....|....*....|....*....|....*....|...
gi 242061796 618 GIYAFVTlvdGVPYSEELRKSLIMTVRNqigAFAAPDKIHWAPGLPKTRSGKI 670
Cdd:cd17654 398 RLIAFIV---GESSSSRIHKELQLTLLS---SHAIPDTFVQIDKLPLTSHGKV 444
|
|
| PRK05620 |
PRK05620 |
long-chain fatty-acid--CoA ligase; |
144-681 |
3.59e-14 |
|
long-chain fatty-acid--CoA ligase;
Pssm-ID: 180167 [Multi-domain] Cd Length: 576 Bit Score: 75.97 E-value: 3.59e-14
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 144 GDKIAMYWEGNEPSQdgkLTYSELLEKVCQLANYLK-SLGVGKGDAVIIYLPMLLELPIAMLACARIGAVHSVVFAGFSA 222
Cdd:PRK05620 24 GDTTVTTWGGAEQEQ---TTFAAIGARAAALAHALHdELGITGDQRVGSMMYNCAEHLEVLFAVACMGAVFNPLNKQLMN 100
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 223 DSL-------AQRIVDCKPKL------VIT-CNAVkRGVKPIFLKDIVDVALVESEknGVSvglCLTYENQSAMKREDTK 288
Cdd:PRK05620 101 DQIvhiinhaEDEVIVADPRLaeqlgeILKeCPCV-RAVVFIGPSDADSAAAHMPE--GIK---VYSYEALLDGRSTVYD 174
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 289 WqPErdvwwqdvvtkfptkcdvewVDAEDPLFLLYTSGSTGKPKGV-------------LHTSGGYMVYTATTFKYAfdy 355
Cdd:PRK05620 175 W-PE--------------------LDETTAAAICYSTGTTGAPKGVvyshrslylqslsLRTTDSLAVTHGESFLCC--- 230
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 356 kpTDIY----WCTADCGWITGHSYVTYGPLLNGATVLvfegtpnypdsgrcwDIVDKYNVTIFYTAPTLVRSLMrdgTEY 431
Cdd:PRK05620 231 --VPIYhvlsWGVPLAAFMSGTPLVFPGPDLSAPTLA---------------KIIATAMPRVAHGVPTLWIQLM---VHY 290
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 432 VTRYSRK-SLRVLGSVGEPINP---SAWRWFYNIvgdsrcPISDTWWQTETG--GFMITPLPGA-----WPQKPGSATFP 500
Cdd:PRK05620 291 LKNPPERmSLQEIYVGGSAVPPiliKAWEERYGV------DVVHVWGMTETSpvGTVARPPSGVsgearWAYRVSQGRFP 364
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 501 FfGVQPVIVDEkGQEIEG--ECSGYLCIKKSW-------------PGAFRTLYGDHERYETTYFKPfAGYYFTGDGCSRD 565
Cdd:PRK05620 365 A-SLEYRIVND-GQVMEStdRNEGEIQVRGNWvtasyyhspteegGGAASTFRGEDVEDANDRFTA-DGWLRTGDVGSVT 441
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 566 KDGYHWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAAVVGVEHEVKGQGIYAFVTLVDGVPYSEELRKSLIMTVRN 645
Cdd:PRK05620 442 RDGFLTIHDRARDVIRSGGEWIYSAQLENYIMAAPEVVECAVIGYPDDKWGERPLAVTVLAPGIEPTRETAERLRDQLRD 521
|
570 580 590
....*....|....*....|....*....|....*.
gi 242061796 646 QIGAFAAPDKIHWAPGLPKTRSGKIMRRILRKIAAR 681
Cdd:PRK05620 522 RLPNWMLPEYWTFVDEIDKTSVGKFDKKDLRQHLAD 557
|
|
| FATP_chFAT1_like |
cd05937 |
Uncharacterized subfamily of bifunctional fatty acid transporter/very-long-chain acyl-CoA ... |
163-677 |
7.58e-14 |
|
Uncharacterized subfamily of bifunctional fatty acid transporter/very-long-chain acyl-CoA synthetase in fungi; Fatty acid transport protein (FATP) transports long-chain or very-long-chain fatty acids across the plasma membrane. FATPs also have fatty acid CoA synthetase activity, thus playing dual roles as fatty acid transporters and its activation enzymes. FATPs are the key players in the trafficking of exogenous fatty acids into the cell and in intracellular fatty acid homeostasis. Members of this family are fungal FATPs, including FAT1 from Cochliobolus heterostrophus.
Pssm-ID: 341260 [Multi-domain] Cd Length: 468 Bit Score: 74.39 E-value: 7.58e-14
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 163 TYSELLEKVCQLANYLKS-LGVGKGDAVIIYLPMLLELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPKLVItcn 241
Cdd:cd05937 7 TYSETYDLVLRYAHWLHDdLGVQAGDFVAIDLTNSPEFVFLWLGLWSIGAAPAFINYNLSGDPLIHCLKLSGSRFVI--- 83
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 242 avkrgvkpiflkdivdvalvesekngvsvglcltyenqsamkredtkwqperdvwwqdvvtkfptkcdvewVDAEDPLFL 321
Cdd:cd05937 84 -----------------------------------------------------------------------VDPDDPAIL 92
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 322 LYTSGSTGKPKGVLHTSGGYMVyTATTFKYAFDYKPTD-IYWC----------TADCGWITGHSYVTYGP---------- 380
Cdd:cd05937 93 IYTSGTTGLPKAAAISWRRTLV-TSNLLSHDLNLKNGDrTYTCmplyhgtaafLGACNCLMSGGTLALSRkfsasqfwkd 171
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 381 -LLNGATVLVFEG--------TPNYPD--------------SGRCWD-IVDKYNVTI---FYTAPtlvrslmrDGTEYVT 433
Cdd:cd05937 172 vRDSGATIIQYVGelcryllsTPPSPYdrdhkvrvawgnglRPDIWErFRERFNVPEigeFYAAT--------EGVFALT 243
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 434 RYSRKSLrVLGSVGEpiNPSAWRWFYN----IVG-DsrcPISDTWWQTETGGFMI-TPLpgawpQKPGSATFPFFGVQpv 507
Cdd:cd05937 244 NHNVGDF-GAGAIGH--HGLIRRWKFEnqvvLVKmD---PETDDPIRDPKTGFCVrAPV-----GEPGEMLGRVPFKN-- 310
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 508 ivdekgqeiegecsgylciKKSWPGAFRTLYGDHERYETTYFKPFAGYYFTGDGCSRDKDGYHWLTGRVDDVINVSGHRI 587
Cdd:cd05937 311 -------------------REAFQGYLHNEDATESKLVRDVFRKGDIYFRTGDLLRQDADGRWYFLDRLGDTFRWKSENV 371
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 588 GTAEVESALVSHPQCAEAAVVGVE---HEvkGQGIYAFVTLVDGVPYSEELRKSLIMTV-RNQIGAFAAPDKIHWAPGLP 663
Cdd:cd05937 372 STTEVADVLGAHPDIAEANVYGVKvpgHD--GRAGCAAITLEESSAVPTEFTKSLLASLaRKNLPSYAVPLFLRLTEEVA 449
|
570
....*....|....
gi 242061796 664 KTRSGKIMRRILRK 677
Cdd:cd05937 450 TTDNHKQQKGVLRD 463
|
|
| LC_FACL_like |
cd05914 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); The members of this family are ... |
158-672 |
2.07e-13 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); The members of this family are bacterial long-chain fatty acid CoA synthetase, most of which are as yet uncharacterized. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.
Pssm-ID: 341240 [Multi-domain] Cd Length: 463 Bit Score: 72.86 E-value: 2.07e-13
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 158 QDGKLTYSELLEKVCQLANYLKSLGVGKGDAVIIYLPMLLELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPKLv 237
Cdd:cd05914 4 GGEPLTYKDLADNIAKFALLLKINGVGTGDRVALMGENRPEWGIAFFAIWTYGAIAVPILAEFTADEVHHILNHSEAKA- 82
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 238 itcnavkrgvkpIFLKDIVDVALvesekngvsvglcltyenqsamkredtkwqperdvwwqdvvtkfptkcdvewvdaed 317
Cdd:cd05914 83 ------------IFVSDEDDVAL--------------------------------------------------------- 93
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 318 plfLLYTSGSTGKPKGVLHTSGGYMVYTATTFKYAFdYKPTDIYWCTADCGWITGHSYVTYGPLLNGATVLVFEGTPN-- 395
Cdd:cd05914 94 ---INYTSGTTGNSKGVMLTYRNIVSNVDGVKEVVL-LGKGDKILSILPLHHIYPLTFTLLLPLLNGAHVVFLDKIPSak 169
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 396 ---------YPDSGRC--WDIVDKYNVTIF-----------YTAPTLVRSLMRDGTEYVTRYSRKSLRVLGSVGEPINPS 453
Cdd:cd05914 170 iialafaqvTPTLGVPvpLVIEKIFKMDIIpkltlkkfkfkLAKKINNRKIRKLAFKKVHEAFGGNIKEFVIGGAKINPD 249
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 454 AWRWFYNIvgdsRCPISDTWWQTETGGFMITPLPGAwpQKPGSATFPFFGVQPVIVDEKGQEIEGE--CSGylcikkswP 531
Cdd:cd05914 250 VEEFLRTI----GFPYTIGYGMTETAPIISYSPPNR--IRLGSAGKVIDGVEVRIDSPDPATGEGEiiVRG--------P 315
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 532 GAFRTLYGDHERYETTYFKpfAGYYFTGDGCSRDKDGYHWLTGRVDDVI-NVSGHRIGTAEVESALVSHPQCAEAAVvgV 610
Cdd:cd05914 316 NVMKGYYKNPEATAEAFDK--DGWFHTGDLGKIDAEGYLYIRGRKKEMIvLSSGKNIYPEEIEAKINNMPFVLESLV--V 391
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 611 EHEVKGQG-IYAFVTLVD----GVPYSEE-LRKSLIMTVRNQIGAFA--APDKIHWAPgLPKTRSGKIMR 672
Cdd:cd05914 392 VQEKKLVAlAYIDPDFLDvkalKQRNIIDaIKWEVRDKVNQKVPNYKkiSKVKIVKEE-FEKTPKGKIKR 460
|
|
| PRK07824 |
PRK07824 |
o-succinylbenzoate--CoA ligase; |
549-677 |
2.20e-13 |
|
o-succinylbenzoate--CoA ligase;
Pssm-ID: 236108 [Multi-domain] Cd Length: 358 Bit Score: 72.00 E-value: 2.20e-13
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 549 FKPFA--GYYFTGDGCSRDkDGYHWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAAVVGVEHEVKGQGIYA-FVTL 625
Cdd:PRK07824 227 PDPFAepGWFRTDDLGALD-DGVLTVLGRADDAISTGGLTVLPQVVEAALATHPAVADCAVFGLPDDRLGQRVVAaVVGD 305
|
90 100 110 120 130
....*....|....*....|....*....|....*....|....*....|..
gi 242061796 626 VDGVPYSEELRKSLIMTvrnqIGAFAAPDKIHWAPGLPKTRSGKIMRRILRK 677
Cdd:PRK07824 306 GGPAPTLEALRAHVART----LDRTAAPRELHVVDELPRRGIGKVDRRALVR 353
|
|
| PRK05857 |
PRK05857 |
fatty acid--CoA ligase; |
159-675 |
4.16e-13 |
|
fatty acid--CoA ligase;
Pssm-ID: 180293 [Multi-domain] Cd Length: 540 Bit Score: 72.35 E-value: 4.16e-13
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 159 DG--KLTYSELLEKVCQLANYLKSLGVGKGDAVIIYLPMLLELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPKL 236
Cdd:PRK05857 37 DGtsALRYRELVAEVGGLAADLRAQSVSRGSRVLVISDNGPETYLSVLACAKLGAIAVMADGNLPIAAIERFCQITDPAA 116
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 237 VITCNAVKRGVK--PIFLKDIVDVAlvesekngVSVGLCLTYENQSaMKREDTKWQPErdvwwqdvvtkfptkcdvewVD 314
Cdd:PRK05857 117 ALVAPGSKMASSavPEALHSIPVIA--------VDIAAVTRESEHS-LDAASLAGNAD--------------------QG 167
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 315 AEDPLFLLYTSGSTGKPKGVLhtsggymVYTATTFKYAFDYKPTDIYWCTadcgWITGHSyvTYGPL-----------LN 383
Cdd:PRK05857 168 SEDPLAMIFTSGTTGEPKAVL-------LANRTFFAVPDILQKEGLNWVT----WVVGET--TYSPLpathigglwwiLT 234
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 384 GAT--VLVFEGTPNypdSGRCWDIVDKYNVTIFYTAPTLVRSLMRDGTeyVTRYSRKSLRVLGSVGEPINPSAWRwFYNI 461
Cdd:PRK05857 235 CLMhgGLCVTGGEN---TTSLLEILTTNAVATTCLVPTLLSKLVSELK--SANATVPSLRLVGYGGSRAIAADVR-FIEA 308
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 462 VGdsrCPISDTWWQTETGGFMITpLP---GAWPQ-KPGSATFPFFGVQPVIVDEKG------QEIEGECSGYLCIKKswP 531
Cdd:PRK05857 309 TG---VRTAQVYGLSETGCTALC-LPtddGSIVKiEAGAVGRPYPGVDVYLAATDGigptapGAGPSASFGTLWIKS--P 382
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 532 GAFRTLYGDHERYETTYFKpfaGYYFTGDGCSRDKDGYHWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAAVvgve 611
Cdd:PRK05857 383 ANMLGYWNNPERTAEVLID---GWVNTGDLLERREDGFFYIKGRSSEMIICGGVNIAPDEVDRIAEGVSGVREAAC---- 455
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 242061796 612 HEVKGQGIYAFVTLVDgVPYSE-------ELRKSLIMTVRNQIGAFAAPDKIHWAPGLPKTRSGKIMRRIL 675
Cdd:PRK05857 456 YEIPDEEFGALVGLAV-VASAEldesaarALKHTIAARFRRESEPMARPSTIVIVTDIPRTQSGKVMRASL 525
|
|
| A_NRPS_TubE_like |
cd05906 |
The adenylation domain (A domain) of a family of nonribosomal peptide synthetases (NRPSs) ... |
162-678 |
1.34e-12 |
|
The adenylation domain (A domain) of a family of nonribosomal peptide synthetases (NRPSs) synthesizing toxins and antitumor agents; The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino)-acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. This family includes NRPSs that synthesize toxins and antitumor agents; for example, TubE for Tubulysine, CrpA for cryptophycin, TdiA for terrequinone A, KtzG for kutzneride, and Vlm1/Vlm2 for Valinomycin. Nonribosomal peptide synthetases are large multifunctional enzymes which synthesize many therapeutically useful peptides. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and, in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
Pssm-ID: 341232 [Multi-domain] Cd Length: 540 Bit Score: 70.77 E-value: 1.34e-12
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 162 LTYSELLEKVCQLANYLKSLGVGKGDAVIIYLPMLLELPIAMLACARIGAVHSVVFAGFSADSLAQRIvdckPKLvitCN 241
Cdd:cd05906 40 QSYQDLLEDARRLAAGLRQLGLRPGDSVILQFDDNEDFIPAFWACVLAGFVPAPLTVPPTYDEPNARL----RKL---RH 112
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 242 AVKRGVKPIFLKDIVDVALVESekngvsvglcLTYENQSAMKREDT---KWQPERDVWWQDVvtkfptkcdvewvDAEDP 318
Cdd:cd05906 113 IWQLLGSPVVLTDAELVAEFAG----------LETLSGLPGIRVLSieeLLDTAADHDLPQS-------------RPDDL 169
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 319 LFLLYTSGSTGKPKGVLHTSGGYMVYTATTFKyAFDYKPTDIY--WCTADcgWITGHSYVTYGPLLNG------ATVLVF 390
Cdd:cd05906 170 ALLMLTSGSTGFPKAVPLTHRNILARSAGKIQ-HNGLTPQDVFlnWVPLD--HVGGLVELHLRAVYLGcqqvhvPTEEIL 246
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 391 EGTPNypdsgrcW-DIVDKYNVTIFYtAPTLVRSLMRDGTEYVT--RYSRKSLRVLGSVGEPINPSAWRWFYNIVGDSRC 467
Cdd:cd05906 247 ADPLR-------WlDLIDRYRVTITW-APNFAFALLNDLLEEIEdgTWDLSSLRYLVNAGEAVVAKTIRRLLRLLEPYGL 318
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 468 P---ISDTWWQTETGGFMITPLPGAWPQKPGSATF-----PFFGVQPVIVDEKGQEIEGECSGYLCIKKswPGAFRTLYG 539
Cdd:cd05906 319 PpdaIRPAFGMTETCSGVIYSRSFPTYDHSQALEFvslgrPIPGVSMRIVDDEGQLLPEGEVGRLQVRG--PVVTKGYYN 396
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 540 DHERYETTYFKpfAGYYFTGD-GCSRDkdGYHWLTGRVDDVINVSGHRIGTAEVESAL-----VSHPQCAEAAVVGVEHE 613
Cdd:cd05906 397 NPEANAEAFTE--DGWFRTGDlGFLDN--GNLTITGRTKDTIIVNGVNYYSHEIEAAVeevpgVEPSFTAAFAVRDPGAE 472
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 242061796 614 VKGQGIYaFVTLVDGVPYSEELRKSLIMTVRNQIGafAAPDK-IHWAPG-LPKTRSGKIMRRILRKI 678
Cdd:cd05906 473 TEELAIF-FVPEYDLQDALSETLRAIRSVVSREVG--VSPAYlIPLPKEeIPKTSLGKIQRSKLKAA 536
|
|
| PRK07445 |
PRK07445 |
O-succinylbenzoic acid--CoA ligase; Reviewed |
549-683 |
9.39e-12 |
|
O-succinylbenzoic acid--CoA ligase; Reviewed
Pssm-ID: 236019 [Multi-domain] Cd Length: 452 Bit Score: 67.71 E-value: 9.39e-12
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 549 FKPFAGYYFTGDGCSRDKDGYHWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAAVVGVEHEVKGQGIYAFVTLVDG 628
Cdd:PRK07445 319 ILDSQGIFETDDLGYLDAQGYLHILGRNSQKIITGGENVYPAEVEAAILATGLVQDVCVLGLPDPHWGEVVTAIYVPKDP 398
|
90 100 110 120 130
....*....|....*....|....*....|....*....|....*....|....*
gi 242061796 629 VPYSEELRksliMTVRNQIGAFAAPDKIHWAPGLPKTRSGKIMRRILRKIAARQL 683
Cdd:PRK07445 399 SISLEELK----TAIKDQLSPFKQPKHWIPVPQLPRNPQGKINRQQLQQIAVQRL 449
|
|
| PRK05691 |
PRK05691 |
peptide synthase; Validated |
162-675 |
1.38e-11 |
|
peptide synthase; Validated
Pssm-ID: 235564 [Multi-domain] Cd Length: 4334 Bit Score: 68.27 E-value: 1.38e-11
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 162 LTYSELLEKVCQLANYLKSLGVGKGDAVIIYLPMLLELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPKLVITcn 241
Cdd:PRK05691 2214 LSYAELDARANRLARALRERGVGPQVRVGLALERSLEMVVGLLAILKAGGAYVPLDPEYPLERLHYMIEDSGIGLLLS-- 2291
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 242 avkrgvkpiflkdivDVALVESekngvsVGlcltyENQSAMKRedtkWQPERDvwwQDVVTKFPtkcdvewvDAEDPL-- 319
Cdd:PRK05691 2292 ---------------DRALFEA------LG-----ELPAGVAR----WCLEDD---AAALAAYS--------DAPLPFls 2330
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 320 ------FLLYTSGSTGKPKGVLHTSGGYMVYTATTFKyAFDYKPTD----IYWCTADCGwitghSYVTYGPLLNGA-TVL 388
Cdd:PRK05691 2331 lpqhqaYLIYTSGSTGKPKGVVVSHGEIAMHCQAVIE-RFGMRADDcelhFYSINFDAA-----SERLLVPLLCGArVVL 2404
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 389 VFEGTpnypdsgrcWD------IVDKYNVTIFYTAPTLVRSLMRdgtEYVTRYSRKSLRVLGSVGEPINPSAWR------ 456
Cdd:PRK05691 2405 RAQGQ---------WGaeeicqLIREQQVSILGFTPSYGSQLAQ---WLAGQGEQLPVRMCITGGEALTGEHLQrirqaf 2472
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 457 ---WFYNIVGdsrcpisdtwwQTETggfMITPLPGAWPQ--KPGSATFPFFGV----QPVIVDEKGQEIEGECSGYLCIk 527
Cdd:PRK05691 2473 apqLFFNAYG-----------PTET---VVMPLACLAPEqlEEGAASVPIGRVvgarVAYILDADLALVPQGATGELYV- 2537
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 528 kswpGAFRTLYGDHERYETTYFK----PFAG----YYFTGDGCSRDKDGYHWLTGRVDDVINVSGHRIGTAEVESALVSH 599
Cdd:PRK05691 2538 ----GGAGLAQGYHDRPGLTAERfvadPFAAdggrLYRTGDLVRLRADGLVEYVGRIDHQVKIRGFRIELGEIESRLLEH 2613
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 242061796 600 PQCAEAAVVGVEHEVKGQGIYAFVTLVDGVPYSE--ELRKSLIMTVRNQIGAFAAPDKIHWAPGLPKTRSGKIMRRIL 675
Cdd:PRK05691 2614 PAVREAVVLALDTPSGKQLAGYLVSAVAGQDDEAqaALREALKAHLKQQLPDYMVPAHLILLDSLPLTANGKLDRRAL 2691
|
|
| A_NRPS_ACVS-like |
cd17648 |
N-(5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine synthase; This family contains ACV ... |
159-675 |
7.22e-11 |
|
N-(5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine synthase; This family contains ACV synthetase (ACVS, EC 6.3.2.26; also known as N-(5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine synthase or delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine synthetase) is involved in medically important antibiotic biosynthesis. ACV synthetase is active in an early step in the penicillin G biosynthesis pathway which involves the formation of the tripeptide 6-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine (ACV); each of the constituent amino acids of the tripeptide ACV are activated as aminoacyl-adenylates with peptide bonds formed through the participation of amino acid thioester intermediates. ACV is then cyclized by the action of isopenicillin N synthase.
Pssm-ID: 341303 [Multi-domain] Cd Length: 453 Bit Score: 65.11 E-value: 7.22e-11
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 159 DGKLTYSELLEKVCQLANYLKSLGVGKGDAVI-IYLPMLLELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPKLV 237
Cdd:cd17648 10 DKRLTYRELNERANRLAHYLLSVAEIRPDDLVgLVLDKSELMIIAILAVWKAGAAYVPIDPSYPDERIQFILEDTGARVV 89
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 238 ITcnavkrgvkpiflkDIVDVAlvesekngvsvglcltyenqsamkredtkwqperdvwwqdvvtkfptkcdvewvdaed 317
Cdd:cd17648 90 IT--------------NSTDLA---------------------------------------------------------- 97
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 318 plFLLYTSGSTGKPKGVLHTSGGyMVYTATTFKYAFDYKptdiywCTADCGWITGHSYV-------TYGPLLNGATVLVF 390
Cdd:cd17648 98 --YAIYTSGTTGKPKGVLVEHGS-VVNLRTSLSERYFGR------DNGDEAVLFFSNYVfdffveqMTLALLNGQKLVVP 168
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 391 EGTPNYpDSGRCWDIVDKYNVTIFYTAPTLVRslmrdgtEYVTRySRKSLRVLGSVGEPINPSAwrwFYNIVGDSRCPIS 470
Cdd:cd17648 169 PDEMRF-DPDRFYAYINREKVTYLSGTPSVLQ-------QYDLA-RLPHLKRVDAAGEEFTAPV---FEKLRSRFAGLII 236
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 471 DTWWQTETGGF-MITPLPGAwPQKPGSATFPFFGV---------QPVIVDEKGQ-EIEGEC--SGY-----LCIKKSWPG 532
Cdd:cd17648 237 NAYGPTETTVTnHKRFFPGD-QRFDKSLGRPVRNTkcyvlndamKRVPVGAVGElYLGGDGvaRGYlnrpeLTAERFLPN 315
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 533 AFRTlygDHERYETTYfkpfAGYYFTGDGCSRDKDGYHWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAAVVGVEH 612
Cdd:cd17648 316 PFQT---EQERARGRN----ARLYKTGDLVRWLPSGELEYLGRNDFQVKIRGQRIEPGEVEAALASYPGVRECAVVAKED 388
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 242061796 613 EVKGQG-----IYAFVTLVDGVPYSEELRKSLimtvRNQIGAFAAPDKIHWAPGLPKTRSGKIMRRIL 675
Cdd:cd17648 389 ASQAQSriqkyLVGYYLPEPGHVPESDLLSFL----RAKLPRYMVPARLVRLEGIPVTINGKLDVRAL 452
|
|
| PRK08315 |
PRK08315 |
AMP-binding domain protein; Validated |
553-685 |
9.04e-11 |
|
AMP-binding domain protein; Validated
Pssm-ID: 236236 [Multi-domain] Cd Length: 559 Bit Score: 64.83 E-value: 9.04e-11
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 553 AGYYFTGDGCSRDKDGYHWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAAVVGVEHEVKGQGIYAFVTLVDGVPYS 632
Cdd:PRK08315 426 DGWMHTGDLAVMDEEGYVNIVGRIKDMIIRGGENIYPREIEEFLYTHPKIQDVQVVGVPDEKYGEEVCAWIILRPGATLT 505
|
90 100 110 120 130
....*....|....*....|....*....|....*....|....*....|....
gi 242061796 633 -EELRKSLimtvRNQIGAFAAPDKIHWAPGLPKTRSGKIMRRILRKIAARQLDE 685
Cdd:PRK08315 506 eEDVRDFC----RGKIAHYKIPRYIRFVDEFPMTVTGKIQKFKMREMMIEELGL 555
|
|
| PRK05691 |
PRK05691 |
peptide synthase; Validated |
320-675 |
4.82e-10 |
|
peptide synthase; Validated
Pssm-ID: 235564 [Multi-domain] Cd Length: 4334 Bit Score: 63.26 E-value: 4.82e-10
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 320 FLLYTSGSTGKPKGVLHTSGGYMVYTATTFKYaFDYKPTDIYWCTADCGWITGHSYVTYGPLLNGATVLVfegtPN--YP 397
Cdd:PRK05691 3873 YVIYTSGSTGLPKGVMVEQRGMLNNQLSKVPY-LALSEADVIAQTASQSFDISVWQFLAAPLFGARVEIV----PNaiAH 3947
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 398 DSGRCWDIVDKYNVTIFYTAPTLVRSLMRDgteyvTRYSRKSLRVLGSVGEPINPS-AWRWF--------YNIVGDSRCP 468
Cdd:PRK05691 3948 DPQGLLAHVQAQGITVLESVPSLIQGMLAE-----DRQALDGLRWMLPTGEAMPPElARQWLqrypqiglVNAYGPAECS 4022
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 469 ISDTWWQTETGGFMITPLPGAWPQKPGsatfpffgvQPVIVDEKGQEIEGECSGYLCIKKSwpGAFRTLYGDHERYETTY 548
Cdd:PRK05691 4023 DDVAFFRVDLASTRGSYLPIGSPTDNN---------RLYLLDEALELVPLGAVGELCVAGT--GVGRGYVGDPLRTALAF 4091
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 549 F-KPFAG----YYFTGDGCSRDKDGYHWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAAvVGVEHEVKGQGIYAFV 623
Cdd:PRK05691 4092 VpHPFGApgerLYRTGDLARRRSDGVLEYVGRIDHQVKIRGYRIELGEIEARLHEQAEVREAA-VAVQEGVNGKHLVGYL 4170
|
330 340 350 360 370
....*....|....*....|....*....|....*....|....*....|..
gi 242061796 624 TLVDGVPYSEELRKSLIMTVRNQIGAFAAPDKIHWAPGLPKTRSGKIMRRIL 675
Cdd:PRK05691 4171 VPHQTVLAQGALLERIKQRLRAELPDYMVPLHWLWLDRLPLNANGKLDRKAL 4222
|
|
| PTZ00216 |
PTZ00216 |
acyl-CoA synthetase; Provisional |
162-381 |
1.49e-09 |
|
acyl-CoA synthetase; Provisional
Pssm-ID: 240316 [Multi-domain] Cd Length: 700 Bit Score: 61.15 E-value: 1.49e-09
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 162 LTYSELLEKVCQLANYLKSLGVGKGDAVIIYLPMLLELPIAMLACARIGAVHSVVFAGFSADSL--AQRIVDCKpklVIT 239
Cdd:PTZ00216 122 ITYAELWERIVNFGRGLAELGLTKGSNVAIYEETRWEWLASIYGIWSQSMVAATVYANLGEDALayALRETECK---AIV 198
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 240 CNAVKrgvkpifLKDIVDVAlveseKNGVSVGLCLTYENQ--SAMKREDTkwqpeRDVWWQDVVTK-----------FPT 306
Cdd:PTZ00216 199 CNGKN-------VPNLLRLM-----KSGGMPNTTIIYLDSlpASVDTEGC-----RLVAWTDVVAKghsagshhplnIPE 261
|
170 180 190 200 210 220 230
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 242061796 307 KCDvewvdaeDPLFLLYTSGSTGKPKGVLHTSGgymvyTATTFKYAFDYKPTDIYWCTADcgwitGHSYVTYGPL 381
Cdd:PTZ00216 262 NND-------DLALIMYTSGTTGDPKGVMHTHG-----SLTAGILALEDRLNDLIGPPEE-----DETYCSYLPL 319
|
|
| PLN02861 |
PLN02861 |
long-chain-fatty-acid-CoA ligase |
162-381 |
2.89e-09 |
|
long-chain-fatty-acid-CoA ligase
Pssm-ID: 178452 [Multi-domain] Cd Length: 660 Bit Score: 60.24 E-value: 2.89e-09
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 162 LTYSELLEKVCQLANYLKSLGVGKGDAVIIYLPMLLELPIAMLACARIG-------------AV-----HSVVFAGFSAD 223
Cdd:PLN02861 78 LTYKEVYDAAIRIGSAIRSRGVNPGDRCGIYGSNCPEWIIAMEACNSQGityvplydtlganAVefiinHAEVSIAFVQE 157
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 224 SLAQRIVDCKPKlvitCNAVKRGVkpIFLKDIVDVALVESEKNGVSvglCLTYENQSAMKREDTKWQPERDvwwQDVVTk 303
Cdd:PLN02861 158 SKISSILSCLPK----CSSNLKTI--VSFGDVSSEQKEEAEELGVS---CFSWEEFSLMGSLDCELPPKQK---TDICT- 224
|
170 180 190 200 210 220 230
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 242061796 304 fptkcdvewvdaedplfLLYTSGSTGKPKGVLHTSGGYMVYTATTFKYAFDykpTDIYWCTADcgwitghSYVTYGPL 381
Cdd:PLN02861 225 -----------------IMYTSGTTGEPKGVILTNRAIIAEVLSTDHLLKV---TDRVATEED-------SYFSYLPL 275
|
|
| LC_FACS_euk1 |
cd17639 |
Eukaryotic long-chain fatty acid CoA synthetase (LC-FACS), including fungal proteins; The ... |
162-637 |
4.37e-09 |
|
Eukaryotic long-chain fatty acid CoA synthetase (LC-FACS), including fungal proteins; The members of this family are eukaryotic fatty acid CoA synthetases (EC 6.2.1.3) that activate fatty acids with chain lengths of 12 to 20 and includes fungal proteins. They act on a wide range of long-chain saturated and unsaturated fatty acids, but the enzymes from different tissues show some variation in specificity. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions. Organisms tend to have multiple isoforms of LC-FACS genes with multiple splice variants. For example, nine genes are found in Arabidopsis and six genes are expressed in mammalian cells. In Schizosaccharomyces pombe, lcf1 gene encodes a new fatty acyl-CoA synthetase that preferentially recognizes myristic acid as a substrate.
Pssm-ID: 341294 [Multi-domain] Cd Length: 507 Bit Score: 59.54 E-value: 4.37e-09
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 162 LTYSELLEKVCQLANYLKSLGVGKGDAVIIYLPMLLELPIAMLACARIGAVHSVVFAGFSADSLAQrivdckpklvitcn 241
Cdd:cd17639 6 MSYAEVWERVLNFGRGLVELGLKPGDKVAIFAETRAEWLITALGCWSQNIPIVTVYATLGEDALIH-------------- 71
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 242 avkrgvkpiflkdivdvALVESEKNGVSvglcltyenqsamkredtkwqperdvwwqdvvtkfptkCDVEwvdAEDPLFL 321
Cdd:cd17639 72 -----------------SLNETECSAIF--------------------------------------TDGK---PDDLACI 93
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 322 LYTSGSTGKPKGVLHTSGGyMVYTATTFkyafdykptdiywctadCGWITGH-----SYVTYGPL-------------LN 383
Cdd:cd17639 94 MYTSGSTGNPKGVMLTHGN-LVAGIAGL-----------------GDRVPELlgpddRYLAYLPLahifelaaenvclYR 155
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 384 GATV-------------------------LVFEGTPnypdsgRCWDIVDKyNV------------TIFYTAPTLVRSLMR 426
Cdd:cd17639 156 GGTIgygsprtltdkskrgckgdltefkpTLMVGVP------AIWDTIRK-GVlaklnpmgglkrTLFWTAYQSKLKALK 228
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 427 DG------TEYVTRYSRKS----LRVLGSVGEPINPSAWRwFYNIVGdsrCPISDTWWQTETGGFMITPLPGAWpqKPGS 496
Cdd:cd17639 229 EGpgtpllDELVFKKVRAAlggrLRYMLSGGAPLSADTQE-FLNIVL---CPVIQGYGLTETCAGGTVQDPGDL--ETGR 302
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 497 ATFPFFGVQPVIVD-EKGQEI--EGECSGYLCIKKswPGAFRTLYGDHERYETTYFKpfAGYYFTGDGCSRDKDGYHWLT 573
Cdd:cd17639 303 VGPPLPCCEIKLVDwEEGGYStdKPPPRGEILIRG--PNVFKGYYKNPEKTKEAFDG--DGWFHTGDIGEFHPDGTLKII 378
|
490 500 510 520 530 540
....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 242061796 574 GRVDD-VINVSGHRIGTAEVESALVSHPQCAEAAVVGVEHEVKgqgIYAFVtlvdgVPYSEELRK 637
Cdd:cd17639 379 DRKKDlVKLQNGEYIALEKLESIYRSNPLVNNICVYADPDKSY---PVAIV-----VPNEKHLTK 435
|
|
| PRK04813 |
PRK04813 |
D-alanine--poly(phosphoribitol) ligase subunit DltA; |
157-334 |
8.84e-09 |
|
D-alanine--poly(phosphoribitol) ligase subunit DltA;
Pssm-ID: 235313 [Multi-domain] Cd Length: 503 Bit Score: 58.37 E-value: 8.84e-09
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 157 SQDGKLTYSELLEKVCQLANYLKSLGVGKGDAVIIYLPMLLELPIAMLACARIGavHSVVFAGFSadSLAQRIvdckpkl 236
Cdd:PRK04813 23 YLGEKLTYGQLKEDSDALAAFIDSLKLPDKSPIIVFGHMSPEMLATFLGAVKAG--HAYIPVDVS--SPAERI------- 91
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 237 vitcNAVKRGVKPIFLKDIVDVALvesEKNGVSVglcLTYENQSAMKREDTKWQPERdvwwqdvvtkfptkcdveWVDAE 316
Cdd:PRK04813 92 ----EMIIEVAKPSLIIATEELPL---EILGIPV---ITLDELKDIFATGNPYDFDH------------------AVKGD 143
|
170
....*....|....*...
gi 242061796 317 DPLFLLYTSGSTGKPKGV 334
Cdd:PRK04813 144 DNYYIIFTSGTTGKPKGV 161
|
|
| LC_FACS_like |
cd17640 |
Long-chain fatty acid CoA synthetase; This family includes long-chain fatty acid (C12-C20) CoA ... |
161-695 |
6.59e-08 |
|
Long-chain fatty acid CoA synthetase; This family includes long-chain fatty acid (C12-C20) CoA synthetases, including an Arabidopsis gene At4g14070 that plays a role in activation and elongation of exogenous fatty acids. FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Eukaryotes generally have multiple isoforms of LC-FACS genes with multiple splice variants. For example, nine genes are found in Arabidopsis and six genes are expressed in mammalian cells. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions.
Pssm-ID: 341295 [Multi-domain] Cd Length: 468 Bit Score: 55.44 E-value: 6.59e-08
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 161 KLTYSELLEKVCQLANYLKSLGVGKGDAVIIYLPMLLELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPKLVItc 240
Cdd:cd17640 5 RITYKDLYQEILDFAAGLRSLGVKAGEKVALFADNSPRWLIADQGIMALGAVDVVRGSDSSVEELLYILNHSESVALV-- 82
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 241 navkrgvkpiflkdivdvalVESEKNgvsvglcltyenqsamkredtkwqperdvwwqDVVTkfptkcdvewvdaedplf 320
Cdd:cd17640 83 --------------------VENDSD--------------------------------DLAT------------------ 92
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 321 LLYTSGSTGKPKGVLHTSGGyMVYTATTFKYAFDYKPTDIYWCTADCgWitgHSY---VTYGPLLNGATVlVFEGTPNYP 397
Cdd:cd17640 93 IIYTSGTTGNPKGVMLTHAN-LLHQIRSLSDIVPPQPGDRFLSILPI-W---HSYersAEYFIFACGCSQ-AYTSIRTLK 166
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 398 DsgrcwDIvDKYNVTIFYTAPTLVRSLMRDGTEYVTRYSRKSLRVLG---SVGE---PIN-----PSAWRWFYNIVGdsr 466
Cdd:cd17640 167 D-----DL-KRVKPHYIVSVPRLWESLYSGIQKQVSKSSPIKQFLFLfflSGGIfkfGISgggalPPHVDTFFEAIG--- 237
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 467 CPISDTWWQTETGGFMITPLPgaWPQKPGSATFPFFGVQPVIVDEKGQEI-EGECSGYLCIKKswPGAFRTLYGDHEryE 545
Cdd:cd17640 238 IEVLNGYGLTETSPVVSARRL--KCNVRGSVGRPLPGTEIKIVDPEGNVVlPPGEKGIVWVRG--PQVMKGYYKNPE--A 311
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 546 TTYFKPFAGYYFTGDGCSRDKDGYHWLTGRVDDVINVS-GHRIGTAEVESALVSHPQCAEAAVVGVEHEVKGQGIyafvt 624
Cdd:cd17640 312 TSKVLDSDGWFNTGDLGWLTCGGELVLTGRAKDTIVLSnGENVEPQPIEEALMRSPFIEQIMVVGQDQKRLGALI----- 386
|
490 500 510 520 530 540 550
....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 242061796 625 lvdgVPYSEELRKSLimtvrNQIGAFAAPDKIHWAPGLPKTR--SGKIMRRILRKIAARQLDELGDISTLAEP 695
Cdd:cd17640 387 ----VPNFEELEKWA-----KESGVKLANDRSQLLASKKVLKlyKNEIKDEISNRPGFKSFEQIAPFALLEEP 450
|
|
| PRK08308 |
PRK08308 |
acyl-CoA synthetase; Validated |
557-691 |
1.01e-06 |
|
acyl-CoA synthetase; Validated
Pssm-ID: 236231 [Multi-domain] Cd Length: 414 Bit Score: 51.58 E-value: 1.01e-06
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 557 FTGDGCSRDKDGYHWLTGRVDDVINVSGHRIGTAEVESALVSHPQCAEAAVVGVEHEVKGQGIYAFVTlVDGVPYSEELR 636
Cdd:PRK08308 294 FTKDLGYKSERGTLHFMGRMDDVINVSGLNVYPIEVEDVMLRLPGVQEAVVYRGKDPVAGERVKAKVI-SHEEIDPVQLR 372
|
90 100 110 120 130
....*....|....*....|....*....|....*....|....*....|....*
gi 242061796 637 KslimTVRNQIGAFAAPDKIHWAPGLPKTRSGKIMRRILrkiaarqldELGDIST 691
Cdd:PRK08308 373 E----WCIQHLAPYQVPHEIESVTEIPKNANGKVSRKLL---------ELGEVTA 414
|
|
| PRK09188 |
PRK09188 |
serine/threonine protein kinase; Provisional |
595-706 |
7.66e-06 |
|
serine/threonine protein kinase; Provisional
Pssm-ID: 236400 [Multi-domain] Cd Length: 365 Bit Score: 48.61 E-value: 7.66e-06
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 595 ALVSHPQCAEAAVVGVEHEVKGQGIYAFVTlVDGVPYSEELRKSLimtvrNQIGAFAAPDKIHWAPGLPKTRSGKIMRRI 674
Cdd:PRK09188 248 ALKSDPAVSDVAIALFSLPAKGVGLYAFVE-AELPADEKSLRARL-----AGAKPPKPPEHIQPVAALPRDADGTVRDDI 321
|
90 100 110
....*....|....*....|....*....|..
gi 242061796 675 LRKIAARQLDELGDIstLAEPAVVDQLIALSD 706
Cdd:PRK09188 322 LRLIAMNQIDELDDL--LREPEIRGLVEAIAA 351
|
|
| FACL_like_1 |
cd05910 |
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ... |
315-675 |
1.03e-05 |
|
Uncharacterized subfamily of fatty acid CoA ligase (FACL); Fatty acyl-CoA ligases catalyze the ATP-dependent activation of fatty acids in a two-step reaction. The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions.
Pssm-ID: 341236 [Multi-domain] Cd Length: 457 Bit Score: 48.61 E-value: 1.03e-05
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 315 AEDPLFLLYTSGSTGKPKGVlhtsggymVYTATTFKYAFDYKPTDIYWCTADCGWITGHSYVTYGPLLnGATVLVFEGTP 394
Cdd:cd05910 84 ADEPAAILFTSGSTGTPKGV--------VYRHGTFAAQIDALRQLYGIRPGEVDLATFPLFALFGPAL-GLTSVIPDMDP 154
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 395 NYP---DSGRCWDIVDKYNVTIFYTAPTLVRSLmrdgTEYVTRYSRK--SLRVLGSVGEPINPSAWRWFYNIVGDSR--- 466
Cdd:cd05910 155 TRParaDPQKLVGAIRQYGVSIVFGSPALLERV----ARYCAQHGITlpSLRRVLSAGAPVPIALAARLRKMLSDEAeil 230
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 467 -----------CPISDTWWQTETggfmiTPLPgawpqKPGSAT---FPFFGVQPVIV---DEKGQEIEGECS------GY 523
Cdd:cd05910 231 tpygatealpvSSIGSRELLATT-----TAAT-----SGGAGTcvgRPIPGVRVRIIeidDEPIAEWDDTLElprgeiGE 300
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 524 LCIkkSWPGAFRTLYGDHEryETTYFKPFAG----YYFTGDGCSRDKDGYHWLTGRVDDVINVSGHRIGTAEVESALVSH 599
Cdd:cd05910 301 ITV--TGPTVTPTYVNRPV--ATALAKIDDNsegfWHRMGDLGYLDDEGRLWFCGRKAHRVITTGGTLYTEPVERVFNTH 376
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 600 PQCAEAAVVGVEHEVKGQGIYAFVTLVDGVPYSEELRKSLIMTVRN-----QIGAFAapdkIHWAPGLPKTRSGKIMRRI 674
Cdd:cd05910 377 PGVRRSALVGVGKPGCQLPVLCVEPLPGTITPRARLEQELRALAKDyphtqRIGRFL----IHPSFPVDIRHNAKIFREK 452
|
.
gi 242061796 675 L 675
Cdd:cd05910 453 L 453
|
|
| PRK07768 |
PRK07768 |
long-chain-fatty-acid--CoA ligase; Validated |
309-683 |
2.13e-05 |
|
long-chain-fatty-acid--CoA ligase; Validated
Pssm-ID: 236091 [Multi-domain] Cd Length: 545 Bit Score: 47.68 E-value: 2.13e-05
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 309 DVEWVDAEDPLFLLYTSGSTGKPKGVLHTSGGyMVYTATTFKYAFDYKP-TD--IYW--CTADCGWItghSYVTYgPLLN 383
Cdd:PRK07768 145 DPVETGEDDLALMQLTSGSTGSPKAVQITHGN-LYANAEAMFVAAEFDVeTDvmVSWlpLFHDMGMV---GFLTV-PMYF 219
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 384 GATVLVfeGTP-NYPDSGRCW-DIVDKYNVTIfYTAP----TLVRSLMRDGTEYvTRYSRKSLRVLGSVGEPINPSAWRW 457
Cdd:PRK07768 220 GAELVK--VTPmDFLRDPLLWaELISKYRGTM-TAAPnfayALLARRLRRQAKP-GAFDLSSLRFALNGAEPIDPADVED 295
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 458 F-----------------YNI------VGDSRCPisdtwwqtetGGFMITP--------LPGAWPQKPGSAT------FP 500
Cdd:PRK07768 296 LldagarfglrpeailpaYGMaeatlaVSFSPCG----------AGLVVDEvdadllaaLRRAVPATKGNTRrlatlgPP 365
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 501 FFGVQPVIVDEKGQ----------EIEGEC--SGYLCikkswPGAFRTLYGDHeryettyfkpfaGYYFTGDGCSRDKDG 568
Cdd:PRK07768 366 LPGLEVRVVDEDGQvlpprgvgviELRGESvtPGYLT-----MDGFIPAQDAD------------GWLDTGDLGYLTEEG 428
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 569 YHWLTGRVDDVINVSGHRIGTAEVESALVS----HPQCAEAavVGVEHEVKGQGIYAFVTLVDGVPYSE--ELRKSLIMT 642
Cdd:PRK07768 429 EVVVCGRVKDVIIMAGRNIYPTDIERAAARvegvRPGNAVA--VRLDAGHSREGFAVAVESNAFEDPAEvrRIRHQVAHE 506
|
410 420 430 440
....*....|....*....|....*....|....*....|...
gi 242061796 643 VRNQIGafAAPDKIH-WAPG-LPKTRSGKimrriLRKIAARQL 683
Cdd:PRK07768 507 VVAEVG--VRPRNVVvLGPGsIPKTPSGK-----LRRANAAEL 542
|
|
| A_NRPS_alphaAR |
cd17647 |
Alpha-aminoadipate reductase; This family contains L-2-aminoadipate reductase, also known as ... |
163-604 |
2.26e-05 |
|
Alpha-aminoadipate reductase; This family contains L-2-aminoadipate reductase, also known as alpha-aminoadipate reductase (EC 1.2.1.95) or alpha-AR or L-aminoadipate-semialdehyde dehydrogenase (EC 1.2.1.31), which catalyzes the activation of alpha-aminoadipate by ATP-dependent adenylation and the reduction of activated alpha-aminoadipate by NADPH. The activated alpha-aminoadipate is bound to the phosphopantheinyl group of the enzyme itself before it is reduced to (S)-2-amino-6-oxohexanoate.
Pssm-ID: 341302 [Multi-domain] Cd Length: 520 Bit Score: 47.51 E-value: 2.26e-05
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 163 TYSELLEKVCQLANYLKSLGVGKGDAVIIYLPMLLELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPKlvitcna 242
Cdd:cd17647 22 TYRDINEASNIVAHYLIKTGIKRGDVVMIYSYRGVDLMVAVMGVLKAGATFSVIDPAYPPARQNIYLGVAKPR------- 94
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 243 vkrgvkpiflkdivdvALVESEKNGVSVGlcltyenqsamkredtkwqPERDvwwqdvvtkfPTkcdvewvdaedplfLL 322
Cdd:cd17647 95 ----------------GLIVIRAAGVVVG-------------------PDSN----------PT--------------LS 115
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 323 YTSGSTGKPKGVL--HTSGGYMV-YTATTFKYAFDYKPTdiywctadcgWITG--HSYV---TYGPLLNGATVLVFE--- 391
Cdd:cd17647 116 FTSGSEGIPKGVLgrHFSLAYYFpWMAKRFNLSENDKFT----------MLSGiaHDPIqrdMFTPLFLGAQLLVPTqdd 185
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 392 -GTPnypdsGRCWDIVDKYNVTIFYTAPTLVRSLMRDGTeyvtrysrkslrvlgsvgEPInPSAWRWFYniVGD------ 464
Cdd:cd17647 186 iGTP-----GRLAEWMAKYGATVTHLTPAMGQLLTAQAT------------------TPF-PKLHHAFF--VGDiltkrd 239
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 465 --------SRCPISDTWWQTET----GGFMITplpgAWPQKPG-----SATFP----FFGVQPVIVD-----------EK 512
Cdd:cd17647 240 clrlqtlaENVRIVNMYGTTETqravSYFEVP----SRSSDPTflknlKDVMPagrgMLNVQLLVVNrndrtqicgigEV 315
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 513 GqEI----EGECSGYLCIK--------KSW---PGAFRTLYGD-HERYETTYFKPFAGYYFTGDGCSRDKDGYHWLTGRV 576
Cdd:cd17647 316 G-EIyvraGGLAEGYRGLPelnkekfvNNWfvePDHWNYLDKDnNEPWRQFWLGPRDRLYRTGDLGRYLPNGDCECCGRA 394
|
490 500
....*....|....*....|....*...
gi 242061796 577 DDVINVSGHRIGTAEVESALVSHPQCAE 604
Cdd:cd17647 395 DDQVKIRGFRIELGEIDTHISQHPLVRE 422
|
|
| PRK09274 |
PRK09274 |
peptide synthase; Provisional |
162-455 |
8.41e-05 |
|
peptide synthase; Provisional
Pssm-ID: 236443 [Multi-domain] Cd Length: 552 Bit Score: 45.66 E-value: 8.41e-05
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 162 LTYSELLEKVCQLANYLKSLGVGKGDAVIIYLPMLLELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPKLVITCN 241
Cdd:PRK09274 42 LSFAELDARSDAIAHGLNAAGIGRGMRAVLMVTPSLEFFALTFALFKAGAVPVLVDPGMGIKNLKQCLAEAQPDAFIGIP 121
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 242 AV----------KRGVKPIflkdivdvalvesekngVSVGLCLTYEnqsaMKREDTkwqPERDvwwqDVVTKFPtkcdVE 311
Cdd:PRK09274 122 KAhlarrlfgwgKPSVRRL-----------------VTVGGRLLWG----GTTLAT---LLRD----GAAAPFP----MA 169
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 312 WVDAEDPLFLLYTSGSTGKPKGVlhtsggymVYTATTF-------KYAFDYKPTDIYWCTADCgwitghsYVTYGPLLNG 384
Cdd:PRK09274 170 DLAPDDMAAILFTSGSTGTPKGV--------VYTHGMFeaqiealREDYGIEPGEIDLPTFPL-------FALFGPALGM 234
|
250 260 270 280 290 300 310
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 242061796 385 ATVlVFEGTPNYP---DSGRCWDIVDKYNVTIFYTAPTLVRSLMRDGTEyvTRYSRKSLRVLGSVGEPINPSAW 455
Cdd:PRK09274 235 TSV-IPDMDPTRPatvDPAKLFAAIERYGVTNLFGSPALLERLGRYGEA--NGIKLPSLRRVISAGAPVPIAVI 305
|
|
| LC-FACS_euk |
cd05927 |
Eukaryotic long-chain fatty acid CoA synthetase (LC-FACS); The members of this family are ... |
162-361 |
1.05e-04 |
|
Eukaryotic long-chain fatty acid CoA synthetase (LC-FACS); The members of this family are eukaryotic fatty acid CoA synthetases that activate fatty acids with chain lengths of 12 to 20. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions. Organisms tend to have multiple isoforms of LC-FACS genes with multiple splice variants. For example, nine genes are found in Arabidopsis and six genes are expressed in mammalian cells.
Pssm-ID: 341250 [Multi-domain] Cd Length: 545 Bit Score: 45.28 E-value: 1.05e-04
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 162 LTYSELLEKVCQLANYLKSLGV--GKGDAVIIYLPMLLELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPKLVIt 239
Cdd:cd05927 6 ISYKEVAERADNIGSALRSLGGkpAPASFVGIYSINRPEWIISELACYAYSLVTVPLYDTLGPEAIEYILNHAEISIVF- 84
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 240 cnaVKRGVKPIFLKDIVDVAlvesEKNgvsvglcltyenqsamkredtkwqperdvwwqdvvtkfptKCDVEWVDAEDPL 319
Cdd:cd05927 85 ---CDAGVKVYSLEEFEKLG----KKN----------------------------------------KVPPPPPKPEDLA 117
|
170 180 190 200
....*....|....*....|....*....|....*....|....*
gi 242061796 320 FLLYTSGSTGKPKGVLHTSGGYMVYTATTFKYAFDYK---PTDIY 361
Cdd:cd05927 118 TICYTSGTTGNPKGVMLTHGNIVSNVAGVFKILEILNkinPTDVY 162
|
|
| PRK06334 |
PRK06334 |
long chain fatty acid--[acyl-carrier-protein] ligase; Validated |
158-444 |
3.15e-04 |
|
long chain fatty acid--[acyl-carrier-protein] ligase; Validated
Pssm-ID: 180533 [Multi-domain] Cd Length: 539 Bit Score: 44.04 E-value: 3.15e-04
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 158 QDGKLTYSELLEKVCQLANYL-----KSLGV---GKGDAVIIYLPMLL--ELPI---------AMLACARIGAVHSVVFA 218
Cdd:PRK06334 42 QLGKLSYNQVRKAVIALATKVskypdQHIGImmpASAGAYIAYFATLLsgKIPVminwsqglrEVTACANLVGVTHVLTS 121
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 219 GFSADSLAQrivdckpklvITCNAVKRGVKPIFLKDIvdvalvesekngvsvglcltyenqsamKREDTkwqperdvWWQ 298
Cdd:PRK06334 122 KQLMQHLAQ----------THGEDAEYPFSLIYMEEV---------------------------RKELS--------FWE 156
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 299 DVVTKFPTKCDVEWV---------DAEDPLFLLYTSGSTGKPKGVLHTSGGYMVYTATTFKYaFDYKPTDIYWCTADCGW 369
Cdd:PRK06334 157 KCRIGIYMSIPFEWLmrwfgvsdkDPEDVAVILFTSGTEKLPKGVPLTHANLLANQRACLKF-FSPKEDDVMMSFLPPFH 235
|
250 260 270 280 290 300 310
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 242061796 370 ITGHSYVTYGPLLNGATVlVFEGTPNYPDsgRCWDIVDKYNVTIFYTAPTLVRSLMRDGTEYVTRYSRKSLRVLG 444
Cdd:PRK06334 236 AYGFNSCTLFPLLSGVPV-VFAYNPLYPK--KIVEMIDEAKVTFLGSTPVFFDYILKTAKKQESCLPSLRFVVIG 307
|
|
| PRK09192 |
PRK09192 |
fatty acyl-AMP ligase; |
162-342 |
2.68e-03 |
|
fatty acyl-AMP ligase;
Pssm-ID: 236403 [Multi-domain] Cd Length: 579 Bit Score: 41.14 E-value: 2.68e-03
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 162 LTYSELLEKVCQLANYLKSLGVGKGDAVIIYLPMLLELPIAMLACARIGAVHS-----VVFAGFSA--DSLAQRIVDCKP 234
Cdd:PRK09192 50 LPYQTLRARAEAGARRLLALGLKPGDRVALIAETDGDFVEAFFACQYAGLVPVplplpMGFGGRESyiAQLRGMLASAQP 129
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 242061796 235 KLVITcnavkrgvkPIFLKDIVDVALvesekNGVSVGLCLTYENQSAMkredtkwqPERDVwwqdvvtKFPTkcdvewVD 314
Cdd:PRK09192 130 AAIIT---------PDELLPWVNEAT-----HGNPLLHVLSHAWFKAL--------PEADV-------ALPR------PT 174
|
170 180
....*....|....*....|....*...
gi 242061796 315 AEDPLFLLYTSGSTGKPKGVLHTSGGYM 342
Cdd:PRK09192 175 PDDIAYLQYSSGSTRFPRGVIITHRALM 202
|
|
| |
